spz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spz
Entrez Gene ID	43256
Full Name	spatzle
Synonyms	CG6134,CT19282,Dmel\CG6134,SPZ,Spz,Spz1,mel(3)7
General protein information	Preferred Names spatzle Names CG6134 gene product from transcript CG6134-RL CG6134-PA CG6134-PB CG6134-PC CG6134-PD CG6134-PI CG6134-PJ CG6134-PK CG6134-PL protein spaetzle precursor spaetzle spazle spaztle spz-PA spz-PB spz-PC spz-PD spz-PI spz-PJ spz-PK spz-PL
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 97E1-97E1\|3-92 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_170312.2	NP_733191.1	spatzle, isoform B
NM_170309.2	NP_733188.1	spatzle, isoform I
NM_170316.2	NP_733195.1	spatzle, isoform D
NM_170313.2	NP_733192.1	spatzle, isoform J
NM_079802.3	NP_524526.1	spatzle, isoform A
NM_170315.2	NP_733194.1	spatzle, isoform C
NM_001144644.2	NP_001138116.1	spatzle, isoform L
NM_001104472.2	NP_001097942.1	spatzle, isoform K

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The crystal structure of a 1:1 (nonsignaling) complex of the full-length Toll receptor ectodomain (ECD) with the Spatzle cystine knot domain dimer, is reported.
Title: Structure of the Toll-Spatzle complex, a molecular hub in Drosophila development and innate immunity.

These results suggest a novel function of retromer in regulating Toll pathway and innate immunity at a step that modulates ligand processing or activity.
Title: Retromer promotes immune quiescence by suppressing Sp?tzle-Toll pathway in Drosophila.

The asymmetric binding mode of Spz to Toll is similar to that of nerve growth factor (NGF) in complex with the p75 neurotrophin receptor but is distinct from that of microbial ligands bound to the Toll-like receptors.
Title: Cytokine Spatzle binds to the Drosophila immunoreceptor Toll with a neurotrophin-like specificity and couples receptor activation.

The study reports the expression and refolding of Easter, and show that the renatured enzyme performs the activation cleavage of two spatzle isoforms.
Title: In vitro maturation of Drosophila melanogaster Sp?tzle protein with refolded Easter reveals a novel cleavage site within the prodomain.

Data suggest that Spz functions with neuron-type specificity at the muscle 4 NMJ, and DNT1 and DNT2 function together at the muscles 6,7 NMJ.
Title: Neuron-type specific functions of DNT1, DNT2 and Spz at the Drosophila neuromuscular junction.

The following spz gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spz cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06275	NM_001104472.2 Latest version!	Drosophila melanogaster spatzle (spz), transcript variant K, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa06276	NM_170309.2 Latest version!	Drosophila melanogaster spatzle (spz), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa06277	NM_170312.2 Latest version!	Drosophila melanogaster spatzle (spz), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa06278	NM_170313.2 Latest version!	Drosophila melanogaster spatzle (spz), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa06279	NM_170315.2 Latest version!	Drosophila melanogaster spatzle (spz), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa06280	NM_079802.3 Latest version!	Drosophila melanogaster spatzle (spz), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa06281	NM_170316.2 Latest version!	Drosophila melanogaster spatzle (spz), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08863	NM_001144644.2 Latest version!	Drosophila melanogaster spatzle (spz), transcript variant L, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spz ( NM_001104472.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097942.1 Drosophila melanogaster spatzle (spz), transcript variant K, mRNA.

Price & Availability↑

CloneID	OFa06275
Clone ID Related Accession (Same CDS sequence)	NM_001104472.2
Accession Version	NM_001104472.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spatzle, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104472.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGATAG TTTATATCAA TATCGAATTA ACGAAGCGAC ACGAGGCCGA GGTGAGGGCA 
GAGAAAACAG TGGCAGACTA TAAGGCCCTT TTGGCTACCA TTAACAATGG CGGACCCGGA 
AAGTCAGCAT CTAATCACTT ACAGTACGAG GCCAAGGAGT ATGAACGTAT CATCAAAGAG 
CTTTTTACAA TAACCAATGA TGAGGGAGTT GTCCTCTTTA ATAGGACGGA CACGGAGGTG 
CAATCCGAAC AGCCGATACC CCCCCGACAT CCATCGGATA CCAAGTATCG GCCACCACAA 
TCCCCAGCTC GTCCTTTGAG GAATGACACT AAAGAGCACA ATCCCTGCGC CAAGGATGAA 
AGCCAACACC TTAGAAACTT CTGCACGAAT GTGGACGACT ATCCGGACCT TTCAGGCCTC 
ACACATAAGC TAAAAAACAA CTTTGCCAAG TTCTTTAGCA ACGATCTTCA GCCCACGGAT 
GTGAGCTCTC GCGTTGGTGG CTCAGACGAG CGATTCCTTT GCAGGAGCAT CAGGAAGCTG 
GTGTACCCAA AAAAGGGCTT GAGGGCGGAC GACACCTGGC AGTTAATTGT CAATAACGAT 
GAGTACAAAC AGGCCATCCA GATCGAAGAG TGCGAAGGAG CGGATCAACC CTGTGACTTC 
GCCGCCAACT TTCCGCAGAG CTATAATCCA ATCTGCAAGC AGCACTACAC ACAGCAGACC 
CTGGCCAGCA TCAAAAGTGA TGGCGAACTG GACGTGGTGC AGAATTCCTT CAAGATCCCT 
TCCTGCTGCA AGTGCGCGTT GAAGACTGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097942.1
CDS	862..1734
Translation	MMTPMWISLFKVLLLLFAFFATIVYINIELTKRHEAEVRAEKTVADYKALLATINNGGPGKSASNHLQYEAKEYERIIKELFTITNDEGVVLFNRTDTEVQSEQPIPPRHPSDTKYRPPQSPARPLRNDTKEHNPCAKDESQHLRNFCTNVDDYPDLSGLTHKLKNNFAKFFSNDLQPTDVSSRVGGSDERFLCRSIRKLVYPKKGLRADDTWQLIVNNDEYKQAIQIEECEGADQPCDFAANFPQSYNPICKQHYTQQTLASIKSDGELDVVQNSFKIPSCCKCALKTG

Target ORF information:

RefSeq Version	NM_001104472.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spatzle (spz), transcript variant K, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104472.2

ORF Insert Sequence:

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGATAG TTTATATCAA TATCGAATTA ACGAAGCGAC ACGAGGCCGA GGTGAGGGCA 
GAGAAAACAG TGGCAGACTA TAAGGCCCTT TTGGCTACCA TTAACAATGG CGGACCCGGA 
AAGTCAGCAT CTAATCACTT ACAGTACGAG GCCAAGGAGT ATGAACGTAT CATCAAAGAG 
CTTTTTACAA TAACCAATGA TGAGGGAGTT GTCCTCTTTA ATAGGACGGA CACGGAGGTG 
CAATCCGAAC AGCCGATACC CCCCCGACAT CCATCGGATA CCAAGTATCG GCCACCACAA 
TCCCCAGCTC GTCCTTTGAG GAATGACACT AAAGAGCACA ATCCCTGCGC CAAGGATGAA 
AGCCAACACC TTAGAAACTT CTGCACGAAT GTGGACGACT ATCCGGACCT TTCAGGCCTC 
ACACATAAGC TAAAAAACAA CTTTGCCAAG TTCTTTAGCA ACGATCTTCA GCCCACGGAT 
GTGAGCTCTC GCGTTGGTGG CTCAGACGAG CGATTCCTTT GCAGGAGCAT CAGGAAGCTG 
GTGTACCCAA AAAAGGGCTT GAGGGCGGAC GACACCTGGC AGTTAATTGT CAATAACGAT 
GAGTACAAAC AGGCCATCCA GATCGAAGAG TGCGAAGGAG CGGATCAACC CTGTGACTTC 
GCCGCCAACT TTCCGCAGAG CTATAATCCA ATCTGCAAGC AGCACTACAC ACAGCAGACC 
CTGGCCAGCA TCAAAAGTGA TGGCGAACTG GACGTGGTGC AGAATTCCTT CAAGATCCCT 
TCCTGCTGCA AGTGCGCGTT GAAGACTGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spz ( NM_170309.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733188.1 Drosophila melanogaster spatzle (spz), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa06276
Clone ID Related Accession (Same CDS sequence)	NM_170309.2
Accession Version	NM_170309.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spatzle, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170309.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGAGGA CGGACACGGA GGTGCAATCC GAACAGCCGA TACCCCCCCG ACATCCATCG 
GATACCTTTG TCTTCCCCGA TTCCCCCATA GCCAAGTATC GGCCACCACA ATCCCCAGCT 
CGTCCTTTGA GGAATGACAC TAAAGAGCAC AATCCCTGCG CCAAGGATGA AAGCCAACAC 
CTTAGAAACT TCTGCACGAA TGTGGACGAC TATCCGGACC TTTCAGGCCT CACACATAAG 
CTAAAAAACA ACTTTGCCAA GTTCTTTAGC AACGATCTTC AGCCCACGGA TGTGAGCTCT 
CGCGTTGGTG GCTCAGACGA GCGATTCCTT TGCAGGAGCA TCAGGAAGCT GGTGTACCCA 
AAAAAGGGCT TGAGGGCGGA CGACACCTGG CAGTTAATTG TCAATAACGA TGAGTACAAA 
CAGGCCATCC AGATCGAAGA GTGCGAAGGA GCGGATCAAC CCTGTGACTT CGCCGCCAAC 
TTTCCGCAGA GCTATAATCC AATCTGCAAG CAGCACTACA CACAGCAGAC CCTGGCCAGC 
ATCAAAAGTG ATGGCGAACT GGACGTGGTG CAGAATTCCT TCAAGATCCC TTCCTGCTGC 
AAGTGCGCGT TGAAGACTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733188.1
CDS	862..1545
Translation	MMTPMWISLFKVLLLLFAFFATRTDTEVQSEQPIPPRHPSDTFVFPDSPIAKYRPPQSPARPLRNDTKEHNPCAKDESQHLRNFCTNVDDYPDLSGLTHKLKNNFAKFFSNDLQPTDVSSRVGGSDERFLCRSIRKLVYPKKGLRADDTWQLIVNNDEYKQAIQIEECEGADQPCDFAANFPQSYNPICKQHYTQQTLASIKSDGELDVVQNSFKIPSCCKCALKTG

Target ORF information:

RefSeq Version	NM_170309.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spatzle (spz), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170309.2

ORF Insert Sequence:

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGAGGA CGGACACGGA GGTGCAATCC GAACAGCCGA TACCCCCCCG ACATCCATCG 
GATACCTTTG TCTTCCCCGA TTCCCCCATA GCCAAGTATC GGCCACCACA ATCCCCAGCT 
CGTCCTTTGA GGAATGACAC TAAAGAGCAC AATCCCTGCG CCAAGGATGA AAGCCAACAC 
CTTAGAAACT TCTGCACGAA TGTGGACGAC TATCCGGACC TTTCAGGCCT CACACATAAG 
CTAAAAAACA ACTTTGCCAA GTTCTTTAGC AACGATCTTC AGCCCACGGA TGTGAGCTCT 
CGCGTTGGTG GCTCAGACGA GCGATTCCTT TGCAGGAGCA TCAGGAAGCT GGTGTACCCA 
AAAAAGGGCT TGAGGGCGGA CGACACCTGG CAGTTAATTG TCAATAACGA TGAGTACAAA 
CAGGCCATCC AGATCGAAGA GTGCGAAGGA GCGGATCAAC CCTGTGACTT CGCCGCCAAC 
TTTCCGCAGA GCTATAATCC AATCTGCAAG CAGCACTACA CACAGCAGAC CCTGGCCAGC 
ATCAAAAGTG ATGGCGAACT GGACGTGGTG CAGAATTCCT TCAAGATCCC TTCCTGCTGC 
AAGTGCGCGT TGAAGACTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spz ( NM_170312.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733191.1 Drosophila melanogaster spatzle (spz), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa06277
Clone ID Related Accession (Same CDS sequence)	NM_170312.2
Accession Version	NM_170312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spatzle, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170312.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGACGT CCGCGGACAG TGCACCCTTC ATGCCCATAC CCACCCAGCA CGACGATCCG 
ACCCAGAAAC AGAAACAGAA TCAGAATCAA TCTCCGATAC CCGAAACGAA CCGCCATTAC 
CATCAGTATC ACAGCCTGAT CCAACCAGAT CAGTACTTCA AAGTGCAGCG CTCCCCGAAT 
GGAAAGCTGA ATCTGGTCTT TAACGATACG TTCGTATCTT TGCAGAGGAC GGACACGGAG 
GTGCAATCCG AACAGCCGAT ACCCCCCCGA CATCCATCGG ATACCTTTGT CTTCCCCGAT 
TCCCCCATAG CCAAGTATCG GCCACCACAA TCCCCAGCTC GTCCTTTGAG GAATGACACT 
AAAGAGCACA ATCCCTGCGC CAAGGATGAA AGCCAACACC TTAGAAACTT CTGCACGAAT 
GTGGACGACT ATCCGGACCT TTCAGGCCTC ACACATAAGC TAAAAAACAA CTTTGCCAAG 
TTCTTTAGCA ACGATCTTCA GCCCACGGAT GTGAGCTCTC GCGTTGGTGG CTCAGACGAG 
CGATTCCTTT GCAGGAGCAT CAGGAAGCTG GTGTACCCAA AAAAGGGCTT GAGGGCGGAC 
GACACCTGGC AGTTAATTGT CAATAACGAT GAGTACAAAC AGGCCATCCA GATCGAAGAG 
TGCGAAGGAG CGGATCAACC CTGTGACTTC GCCGCCAACT TTCCGCAGAG CTATAATCCA 
ATCTGCAAGC AGCACTACAC ACAGCAGACC CTGGCCAGCA TCAAAAGTGA TGGCGAACTG 
GACGTGGTGC AGAATTCCTT CAAGATCCCT TCCTGCTGCA AGTGCGCGTT GAAGACTGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733191.1
CDS	862..1764
Translation	MMTPMWISLFKVLLLLFAFFATTSADSAPFMPIPTQHDDPTQKQKQNQNQSPIPETNRHYHQYHSLIQPDQYFKVQRSPNGKLNLVFNDTFVSLQRTDTEVQSEQPIPPRHPSDTFVFPDSPIAKYRPPQSPARPLRNDTKEHNPCAKDESQHLRNFCTNVDDYPDLSGLTHKLKNNFAKFFSNDLQPTDVSSRVGGSDERFLCRSIRKLVYPKKGLRADDTWQLIVNNDEYKQAIQIEECEGADQPCDFAANFPQSYNPICKQHYTQQTLASIKSDGELDVVQNSFKIPSCCKCALKTG

Target ORF information:

RefSeq Version	NM_170312.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spatzle (spz), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170312.2

ORF Insert Sequence:

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGACGT CCGCGGACAG TGCACCCTTC ATGCCCATAC CCACCCAGCA CGACGATCCG 
ACCCAGAAAC AGAAACAGAA TCAGAATCAA TCTCCGATAC CCGAAACGAA CCGCCATTAC 
CATCAGTATC ACAGCCTGAT CCAACCAGAT CAGTACTTCA AAGTGCAGCG CTCCCCGAAT 
GGAAAGCTGA ATCTGGTCTT TAACGATACG TTCGTATCTT TGCAGAGGAC GGACACGGAG 
GTGCAATCCG AACAGCCGAT ACCCCCCCGA CATCCATCGG ATACCTTTGT CTTCCCCGAT 
TCCCCCATAG CCAAGTATCG GCCACCACAA TCCCCAGCTC GTCCTTTGAG GAATGACACT 
AAAGAGCACA ATCCCTGCGC CAAGGATGAA AGCCAACACC TTAGAAACTT CTGCACGAAT 
GTGGACGACT ATCCGGACCT TTCAGGCCTC ACACATAAGC TAAAAAACAA CTTTGCCAAG 
TTCTTTAGCA ACGATCTTCA GCCCACGGAT GTGAGCTCTC GCGTTGGTGG CTCAGACGAG 
CGATTCCTTT GCAGGAGCAT CAGGAAGCTG GTGTACCCAA AAAAGGGCTT GAGGGCGGAC 
GACACCTGGC AGTTAATTGT CAATAACGAT GAGTACAAAC AGGCCATCCA GATCGAAGAG 
TGCGAAGGAG CGGATCAACC CTGTGACTTC GCCGCCAACT TTCCGCAGAG CTATAATCCA 
ATCTGCAAGC AGCACTACAC ACAGCAGACC CTGGCCAGCA TCAAAAGTGA TGGCGAACTG 
GACGTGGTGC AGAATTCCTT CAAGATCCCT TCCTGCTGCA AGTGCGCGTT GAAGACTGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spz ( NM_170313.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733192.1 Drosophila melanogaster spatzle (spz), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa06278
Clone ID Related Accession (Same CDS sequence)	NM_170313.2
Accession Version	NM_170313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spatzle, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170313.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGTACG AGGCCAAGGA GTATGAACGT ATCATCAAAG AGCTTTTTAC AATAACCAAT 
GATGAGGGAG TTGTCCTCTT TAATAGGACG GACACGGAGG TGCAATCCGA ACAGCCGATA 
CCCCCCCGAC ATCCATCGGA TACCAAGTAT CGGCCACCAC AATCCCCAGC TCGTCCTTTG 
AGGAATGACA CTAAAGAGCA CAATCCCTGC GCCAAGGATG AAAGCCAACA CCTTAGAAAC 
TTCTGCACGA ATGTGGACGA CTATCCGGAC CTTTCAGGCC TCACACATAA GCTAAAAAAC 
AACTTTGCCA AGTTCTTTAG CAACGATCTT CAGCCCACGG ATGTGAGCTC TCGCGTTGGT 
GGCTCAGACG AGCGATTCCT TTGCAGGAGC ATCAGGAAGC TGGTGTACCC AAAAAAGGGC 
TTGAGGGCGG ACGACACCTG GCAGTTAATT GTCAATAACG ATGAGTACAA ACAGGCCATC 
CAGATCGAAG AGTGCGAAGG AGCGGATCAA CCCTGTGACT TCGCCGCCAA CTTTCCGCAG 
AGCTATAATC CAATCTGCAA GCAGCACTAC ACACAGCAGA CCCTGGCCAG CATCAAAAGT 
GATGGCGAAC TGGACGTGGT GCAGAATTCC TTCAAGATCC CTTCCTGCTG CAAGTGCGCG 
TTGAAGACTG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733192.1
CDS	862..1596
Translation	MMTPMWISLFKVLLLLFAFFATYEAKEYERIIKELFTITNDEGVVLFNRTDTEVQSEQPIPPRHPSDTKYRPPQSPARPLRNDTKEHNPCAKDESQHLRNFCTNVDDYPDLSGLTHKLKNNFAKFFSNDLQPTDVSSRVGGSDERFLCRSIRKLVYPKKGLRADDTWQLIVNNDEYKQAIQIEECEGADQPCDFAANFPQSYNPICKQHYTQQTLASIKSDGELDVVQNSFKIPSCCKCALKTG

Target ORF information:

RefSeq Version	NM_170313.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spatzle (spz), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170313.2

ORF Insert Sequence:

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGTACG AGGCCAAGGA GTATGAACGT ATCATCAAAG AGCTTTTTAC AATAACCAAT 
GATGAGGGAG TTGTCCTCTT TAATAGGACG GACACGGAGG TGCAATCCGA ACAGCCGATA 
CCCCCCCGAC ATCCATCGGA TACCAAGTAT CGGCCACCAC AATCCCCAGC TCGTCCTTTG 
AGGAATGACA CTAAAGAGCA CAATCCCTGC GCCAAGGATG AAAGCCAACA CCTTAGAAAC 
TTCTGCACGA ATGTGGACGA CTATCCGGAC CTTTCAGGCC TCACACATAA GCTAAAAAAC 
AACTTTGCCA AGTTCTTTAG CAACGATCTT CAGCCCACGG ATGTGAGCTC TCGCGTTGGT 
GGCTCAGACG AGCGATTCCT TTGCAGGAGC ATCAGGAAGC TGGTGTACCC AAAAAAGGGC 
TTGAGGGCGG ACGACACCTG GCAGTTAATT GTCAATAACG ATGAGTACAA ACAGGCCATC 
CAGATCGAAG AGTGCGAAGG AGCGGATCAA CCCTGTGACT TCGCCGCCAA CTTTCCGCAG 
AGCTATAATC CAATCTGCAA GCAGCACTAC ACACAGCAGA CCCTGGCCAG CATCAAAAGT 
GATGGCGAAC TGGACGTGGT GCAGAATTCC TTCAAGATCC CTTCCTGCTG CAAGTGCGCG 
TTGAAGACTG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spz ( NM_170315.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733194.1 Drosophila melanogaster spatzle (spz), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa06279
Clone ID Related Accession (Same CDS sequence)	NM_170315.2
Accession Version	NM_170315.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spatzle, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170315.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGTACG AGGCCAAGGA GTATGAACGT ATCATCAAAG AGCTTTTTAC AATAACCAAT 
GATGAGGGAG TTGTCCTCTT TAATAGGACG GACACGGAGG TGCAATCCGA ACAGCCGATA 
CCCCCCCGAC ATCCATCGGA TACCTTTGTC TTCCCCGATT CCCCCATAGC CAAGTATCGG 
CCACCACAAT CCCCAGCTCG TCCTTTGAGG AATGACACTA AAGAGCACAA TCCCTGCGCC 
AAGGATGAAA GCCAACACCT TAGAAACTTC TGCACGAATG TGGACGACTA TCCGGACCTT 
TCAGGCCTCA CACATAAGCT AAAAAACAAC TTTGCCAAGT TCTTTAGCAA CGATCTTCAG 
CCCACGGATG TGAGCTCTCG CGTTGGTGGC TCAGACGAGC GATTCCTTTG CAGGAGCATC 
AGGAAGCTGG TGTACCCAAA AAAGGGCTTG AGGGCGGACG ACACCTGGCA GTTAATTGTC 
AATAACGATG AGTACAAACA GGCCATCCAG ATCGAAGAGT GCGAAGGAGC GGATCAACCC 
TGTGACTTCG CCGCCAACTT TCCGCAGAGC TATAATCCAA TCTGCAAGCA GCACTACACA 
CAGCAGACCC TGGCCAGCAT CAAAAGTGAT GGCGAACTGG ACGTGGTGCA GAATTCCTTC 
AAGATCCCTT CCTGCTGCAA GTGCGCGTTG AAGACTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733194.1
CDS	862..1623
Translation	MMTPMWISLFKVLLLLFAFFATYEAKEYERIIKELFTITNDEGVVLFNRTDTEVQSEQPIPPRHPSDTFVFPDSPIAKYRPPQSPARPLRNDTKEHNPCAKDESQHLRNFCTNVDDYPDLSGLTHKLKNNFAKFFSNDLQPTDVSSRVGGSDERFLCRSIRKLVYPKKGLRADDTWQLIVNNDEYKQAIQIEECEGADQPCDFAANFPQSYNPICKQHYTQQTLASIKSDGELDVVQNSFKIPSCCKCALKTG

Target ORF information:

RefSeq Version	NM_170315.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spatzle (spz), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170315.2

ORF Insert Sequence:

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGTACG AGGCCAAGGA GTATGAACGT ATCATCAAAG AGCTTTTTAC AATAACCAAT 
GATGAGGGAG TTGTCCTCTT TAATAGGACG GACACGGAGG TGCAATCCGA ACAGCCGATA 
CCCCCCCGAC ATCCATCGGA TACCTTTGTC TTCCCCGATT CCCCCATAGC CAAGTATCGG 
CCACCACAAT CCCCAGCTCG TCCTTTGAGG AATGACACTA AAGAGCACAA TCCCTGCGCC 
AAGGATGAAA GCCAACACCT TAGAAACTTC TGCACGAATG TGGACGACTA TCCGGACCTT 
TCAGGCCTCA CACATAAGCT AAAAAACAAC TTTGCCAAGT TCTTTAGCAA CGATCTTCAG 
CCCACGGATG TGAGCTCTCG CGTTGGTGGC TCAGACGAGC GATTCCTTTG CAGGAGCATC 
AGGAAGCTGG TGTACCCAAA AAAGGGCTTG AGGGCGGACG ACACCTGGCA GTTAATTGTC 
AATAACGATG AGTACAAACA GGCCATCCAG ATCGAAGAGT GCGAAGGAGC GGATCAACCC 
TGTGACTTCG CCGCCAACTT TCCGCAGAGC TATAATCCAA TCTGCAAGCA GCACTACACA 
CAGCAGACCC TGGCCAGCAT CAAAAGTGAT GGCGAACTGG ACGTGGTGCA GAATTCCTTC 
AAGATCCCTT CCTGCTGCAA GTGCGCGTTG AAGACTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spz ( NM_079802.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524526.1 Drosophila melanogaster spatzle (spz), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06280
Clone ID Related Accession (Same CDS sequence)	NM_079802.3
Accession Version	NM_079802.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spatzle, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079802.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGTACG AGGCCAAGGA GTATGAACGT ATCATCAAAG AGCTTTTTAC AATAACCAAT 
GATGAGGGAG TTGTCCTCTT TAATACGTCC GCGGACAGTG CACCCTTCAT GCCCATACCC 
ACCCAGCACG ACGATCCGAC CCAGAAACAG AAACAGAATC AGAATCAATC TCCGATACCC 
GAAACGAACC GCCATTACCA TCAGTATCAC AGCCTGATCC AACCAGATCA GTACTTCAAA 
GTGCAGCGCT CCCCGAATGG AAAGCTGAAT CTGGTCTTTA ACGATACGTT CGTATCTTTG 
CAGAGGACGG ACACGGAGGT GCAATCCGAA CAGCCGATAC CCCCCCGACA TCCATCGGAT 
ACCTTTGTCT TCCCCGATTC CCCCATAGCC AAGTATCGGC CACCACAATC CCCAGCTCGT 
CCTTTGAGGA ATGACACTAA AGAGCACAAT CCCTGCGCCA AGGATGAAAG CCAACACCTT 
AGAAACTTCT GCACGAATGT GGACGACTAT CCGGACCTTT CAGGCCTCAC ACATAAGCTA 
AAAAACAACT TTGCCAAGTT CTTTAGCAAC GATCTTCAGC CCACGGATGT GAGCTCTCGC 
GTTGGTGGCT CAGACGAGCG ATTCCTTTGC AGGAGCATCA GGAAGCTGGT GTACCCAAAA 
AAGGGCTTGA GGGCGGACGA CACCTGGCAG TTAATTGTCA ATAACGATGA GTACAAACAG 
GCCATCCAGA TCGAAGAGTG CGAAGGAGCG GATCAACCCT GTGACTTCGC CGCCAACTTT 
CCGCAGAGCT ATAATCCAAT CTGCAAGCAG CACTACACAC AGCAGACCCT GGCCAGCATC 
AAAAGTGATG GCGAACTGGA CGTGGTGCAG AATTCCTTCA AGATCCCTTC CTGCTGCAAG 
TGCGCGTTGA AGACTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524526.1
CDS	862..1842
Translation	MMTPMWISLFKVLLLLFAFFATYEAKEYERIIKELFTITNDEGVVLFNTSADSAPFMPIPTQHDDPTQKQKQNQNQSPIPETNRHYHQYHSLIQPDQYFKVQRSPNGKLNLVFNDTFVSLQRTDTEVQSEQPIPPRHPSDTFVFPDSPIAKYRPPQSPARPLRNDTKEHNPCAKDESQHLRNFCTNVDDYPDLSGLTHKLKNNFAKFFSNDLQPTDVSSRVGGSDERFLCRSIRKLVYPKKGLRADDTWQLIVNNDEYKQAIQIEECEGADQPCDFAANFPQSYNPICKQHYTQQTLASIKSDGELDVVQNSFKIPSCCKCALKTG

Target ORF information:

RefSeq Version	NM_079802.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spatzle (spz), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079802.3

ORF Insert Sequence:

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGTACG AGGCCAAGGA GTATGAACGT ATCATCAAAG AGCTTTTTAC AATAACCAAT 
GATGAGGGAG TTGTCCTCTT TAATACGTCC GCGGACAGTG CACCCTTCAT GCCCATACCC 
ACCCAGCACG ACGATCCGAC CCAGAAACAG AAACAGAATC AGAATCAATC TCCGATACCC 
GAAACGAACC GCCATTACCA TCAGTATCAC AGCCTGATCC AACCAGATCA GTACTTCAAA 
GTGCAGCGCT CCCCGAATGG AAAGCTGAAT CTGGTCTTTA ACGATACGTT CGTATCTTTG 
CAGAGGACGG ACACGGAGGT GCAATCCGAA CAGCCGATAC CCCCCCGACA TCCATCGGAT 
ACCTTTGTCT TCCCCGATTC CCCCATAGCC AAGTATCGGC CACCACAATC CCCAGCTCGT 
CCTTTGAGGA ATGACACTAA AGAGCACAAT CCCTGCGCCA AGGATGAAAG CCAACACCTT 
AGAAACTTCT GCACGAATGT GGACGACTAT CCGGACCTTT CAGGCCTCAC ACATAAGCTA 
AAAAACAACT TTGCCAAGTT CTTTAGCAAC GATCTTCAGC CCACGGATGT GAGCTCTCGC 
GTTGGTGGCT CAGACGAGCG ATTCCTTTGC AGGAGCATCA GGAAGCTGGT GTACCCAAAA 
AAGGGCTTGA GGGCGGACGA CACCTGGCAG TTAATTGTCA ATAACGATGA GTACAAACAG 
GCCATCCAGA TCGAAGAGTG CGAAGGAGCG GATCAACCCT GTGACTTCGC CGCCAACTTT 
CCGCAGAGCT ATAATCCAAT CTGCAAGCAG CACTACACAC AGCAGACCCT GGCCAGCATC 
AAAAGTGATG GCGAACTGGA CGTGGTGCAG AATTCCTTCA AGATCCCTTC CTGCTGCAAG 
TGCGCGTTGA AGACTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spz ( NM_170316.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733195.1 Drosophila melanogaster spatzle (spz), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa06281
Clone ID Related Accession (Same CDS sequence)	NM_170316.2
Accession Version	NM_170316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spatzle, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170316.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGATAG TTTATATCAA TATCGAATTA ACGAAGCGAC ACGAGGCCGA GGTGAGGGCA 
GAGAAAACAG TGGCAGACTA TAAGGCCCTT TTGGCTACCA TTAACAATGG CGGACCCGGA 
AAGTCAGCAT CTAATCACTT ACAGAGGACG GACACGGAGG TGCAATCCGA ACAGCCGATA 
CCCCCCCGAC ATCCATCGGA TACCTTTGTC TTCCCCGATT CCCCCATAGC CAAGTATCGG 
CCACCACAAT CCCCAGCTCG TCCTTTGAGG AATGACACTA AAGAGCACAA TCCCTGCGCC 
AAGGATGAAA GCCAACACCT TAGAAACTTC TGCACGAATG TGGACGACTA TCCGGACCTT 
TCAGGCCTCA CACATAAGCT AAAAAACAAC TTTGCCAAGT TCTTTAGCAA CGATCTTCAG 
CCCACGGATG TGAGCTCTCG CGTTGGTGGC TCAGACGAGC GATTCCTTTG CAGGAGCATC 
AGGAAGCTGG TGTACCCAAA AAAGGGCTTG AGGGCGGACG ACACCTGGCA GTTAATTGTC 
AATAACGATG AGTACAAACA GGCCATCCAG ATCGAAGAGT GCGAAGGAGC GGATCAACCC 
TGTGACTTCG CCGCCAACTT TCCGCAGAGC TATAATCCAA TCTGCAAGCA GCACTACACA 
CAGCAGACCC TGGCCAGCAT CAAAAGTGAT GGCGAACTGG ACGTGGTGCA GAATTCCTTC 
AAGATCCCTT CCTGCTGCAA GTGCGCGTTG AAGACTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733195.1
CDS	862..1683
Translation	MMTPMWISLFKVLLLLFAFFATIVYINIELTKRHEAEVRAEKTVADYKALLATINNGGPGKSASNHLQRTDTEVQSEQPIPPRHPSDTFVFPDSPIAKYRPPQSPARPLRNDTKEHNPCAKDESQHLRNFCTNVDDYPDLSGLTHKLKNNFAKFFSNDLQPTDVSSRVGGSDERFLCRSIRKLVYPKKGLRADDTWQLIVNNDEYKQAIQIEECEGADQPCDFAANFPQSYNPICKQHYTQQTLASIKSDGELDVVQNSFKIPSCCKCALKTG

Target ORF information:

RefSeq Version	NM_170316.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spatzle (spz), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170316.2

ORF Insert Sequence:

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGATAG TTTATATCAA TATCGAATTA ACGAAGCGAC ACGAGGCCGA GGTGAGGGCA 
GAGAAAACAG TGGCAGACTA TAAGGCCCTT TTGGCTACCA TTAACAATGG CGGACCCGGA 
AAGTCAGCAT CTAATCACTT ACAGAGGACG GACACGGAGG TGCAATCCGA ACAGCCGATA 
CCCCCCCGAC ATCCATCGGA TACCTTTGTC TTCCCCGATT CCCCCATAGC CAAGTATCGG 
CCACCACAAT CCCCAGCTCG TCCTTTGAGG AATGACACTA AAGAGCACAA TCCCTGCGCC 
AAGGATGAAA GCCAACACCT TAGAAACTTC TGCACGAATG TGGACGACTA TCCGGACCTT 
TCAGGCCTCA CACATAAGCT AAAAAACAAC TTTGCCAAGT TCTTTAGCAA CGATCTTCAG 
CCCACGGATG TGAGCTCTCG CGTTGGTGGC TCAGACGAGC GATTCCTTTG CAGGAGCATC 
AGGAAGCTGG TGTACCCAAA AAAGGGCTTG AGGGCGGACG ACACCTGGCA GTTAATTGTC 
AATAACGATG AGTACAAACA GGCCATCCAG ATCGAAGAGT GCGAAGGAGC GGATCAACCC 
TGTGACTTCG CCGCCAACTT TCCGCAGAGC TATAATCCAA TCTGCAAGCA GCACTACACA 
CAGCAGACCC TGGCCAGCAT CAAAAGTGAT GGCGAACTGG ACGTGGTGCA GAATTCCTTC 
AAGATCCCTT CCTGCTGCAA GTGCGCGTTG AAGACTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spz ( NM_001144644.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138116.1 Drosophila melanogaster spatzle (spz), transcript variant L, mRNA.

Price & Availability↑

CloneID	OFa08863
Clone ID Related Accession (Same CDS sequence)	NM_001144644.2
Accession Version	NM_001144644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spatzle, isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001144644.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGACGT CCGCGGACAG TGCACCCTTC ATGCCCATAC CCACCCAGCA CGACGATCCG 
ACCCAGAAAC AGAAACAGAA TCAGAATCAA TCTCCGATAC CCGAAACGAA CCGCCATTAC 
CATCAGTATC ACAGCCTGAT CCAACCAGAT CAGTACTTCA AAAGGACGGA CACGGAGGTG 
CAATCCGAAC AGCCGATACC CCCCCGACAT CCATCGGATA CCAAGTATCG GCCACCACAA 
TCCCCAGCTC GTCCTTTGAG GAATGACACT AAAGAGCACA ATCCCTGCGC CAAGGATGAA 
AGCCAACACC TTAGAAACTT CTGCACGAAT GTGGACGACT ATCCGGACCT TTCAGGCCTC 
ACACATAAGC TAAAAAACAA CTTTGCCAAG TTCTTTAGCA ACGATCTTCA GCCCACGGAT 
GTGAGCTCTC GCGTTGGTGG CTCAGACGAG CGATTCCTTT GCAGGAGCAT CAGGAAGCTG 
GTGTACCCAA AAAAGGGCTT GAGGGCGGAC GACACCTGGC AGTTAATTGT CAATAACGAT 
GAGTACAAAC AGGCCATCCA GATCGAAGAG TGCGAAGGAG CGGATCAACC CTGTGACTTC 
GCCGCCAACT TTCCGCAGAG CTATAATCCA ATCTGCAAGC AGCACTACAC ACAGCAGACC 
CTGGCCAGCA TCAAAAGTGA TGGCGAACTG GACGTGGTGC AGAATTCCTT CAAGATCCCT 
TCCTGCTGCA AGTGCGCGTT GAAGACTGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138116.1
CDS	862..1674
Translation	MMTPMWISLFKVLLLLFAFFATTSADSAPFMPIPTQHDDPTQKQKQNQNQSPIPETNRHYHQYHSLIQPDQYFKRTDTEVQSEQPIPPRHPSDTKYRPPQSPARPLRNDTKEHNPCAKDESQHLRNFCTNVDDYPDLSGLTHKLKNNFAKFFSNDLQPTDVSSRVGGSDERFLCRSIRKLVYPKKGLRADDTWQLIVNNDEYKQAIQIEECEGADQPCDFAANFPQSYNPICKQHYTQQTLASIKSDGELDVVQNSFKIPSCCKCALKTG

Target ORF information:

RefSeq Version	NM_001144644.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spatzle (spz), transcript variant L, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144644.2

ORF Insert Sequence:

ATGATGACGC CCATGTGGAT ATCGCTGTTC AAAGTCCTGC TGCTGCTGTT CGCCTTCTTT 
GCCACGACGT CCGCGGACAG TGCACCCTTC ATGCCCATAC CCACCCAGCA CGACGATCCG 
ACCCAGAAAC AGAAACAGAA TCAGAATCAA TCTCCGATAC CCGAAACGAA CCGCCATTAC 
CATCAGTATC ACAGCCTGAT CCAACCAGAT CAGTACTTCA AAAGGACGGA CACGGAGGTG 
CAATCCGAAC AGCCGATACC CCCCCGACAT CCATCGGATA CCAAGTATCG GCCACCACAA 
TCCCCAGCTC GTCCTTTGAG GAATGACACT AAAGAGCACA ATCCCTGCGC CAAGGATGAA 
AGCCAACACC TTAGAAACTT CTGCACGAAT GTGGACGACT ATCCGGACCT TTCAGGCCTC 
ACACATAAGC TAAAAAACAA CTTTGCCAAG TTCTTTAGCA ACGATCTTCA GCCCACGGAT 
GTGAGCTCTC GCGTTGGTGG CTCAGACGAG CGATTCCTTT GCAGGAGCAT CAGGAAGCTG 
GTGTACCCAA AAAAGGGCTT GAGGGCGGAC GACACCTGGC AGTTAATTGT CAATAACGAT 
GAGTACAAAC AGGCCATCCA GATCGAAGAG TGCGAAGGAG CGGATCAACC CTGTGACTTC 
GCCGCCAACT TTCCGCAGAG CTATAATCCA ATCTGCAAGC AGCACTACAC ACAGCAGACC 
CTGGCCAGCA TCAAAAGTGA TGGCGAACTG GACGTGGTGC AGAATTCCTT CAAGATCCCT 
TCCTGCTGCA AGTGCGCGTT GAAGACTGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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