SPARC cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPARC
Entrez Gene ID	43230
Full Name	Secreted protein, acidic, cysteine-rich
Synonyms	BM-40 Sparc,BM-40-SPARC,BM-40/SPARC,BM40,CG6378,CT19876,Dmel\CG6378,Sparc,anon-EST:Posey291,dSPARC,dSparc,dsparc,sparc
General protein information	Preferred Names Secreted protein, acidic, cysteine-rich Names CG6378 gene product from transcript CG6378-RA BM-40-SPARC BM-40-secreted protein acidic and rich in cysteine CG6378-PA CG6378-PB SPARC-PA SPARC-PB osteonectin secreted protein, acidic, cysteine-rich
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 97D3-97D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_143252.4	NP_651509.2	secreted protein, acidic, cysteine-rich, isoform A
NM_001276069.1	NP_001262998.1	secreted protein, acidic, cysteine-rich, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPARCL1	NP_004675.3
	SPARCL1	XP_001098328.2
Mus musculus (house mouse)	Sparcl1	NP_034227.3
Drosophila melanogaster (fruit fly)	BM-40-SPARC	NP_001262998.1
Caenorhabditis elegans (roundworm)	ost-1	NP_500039.1
Pan troglodytes (chimpanzee)	SPARCL1	NP_001092036.1
Bos taurus (cattle)	SPARCL1	NP_001029474.1
Canis lupus familiaris (dog)	SPARCL1	XP_005639220.1
Rattus norvegicus (Norway rat)	Sparcl1	NP_037078.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000305	XP_001237311.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data implicate SPARC in the noncell autonomous control of cardiac function in Drosophila and suggest that modulation of SPARC gene expression may ameliorate cardiac dysfunction in humans.
Title: SPARC-Dependent Cardiomyopathy in Drosophila.

Egg chamber elongation requires temporal down-regulation of SPARC.
Title: Dynamic regulation of basement membrane protein levels promotes egg chamber elongation in Drosophila.

SPARC has a critical role in the proper basal lamina assembly in Drosophila fat body
Title: Loss of SPARC dysregulates basal lamina assembly to disrupt larval fat body homeostasis in Drosophila melanogaster.

Drosophila Sparc is identified as an early marker transcriptionally upregulated in loser cells that provides a transient protection by inhibiting Caspase activation in outcompeted cells.
Title: Drosophila SPARC is a self-protective signal expressed by loser cells during cell competition.

SPARC is required for basal lamina maturation and condensation of the ventral nerve cord during Drosophila embryogenesis.
Title: Haemocyte-derived SPARC is required for collagen-IV-dependent stability of basal laminae in Drosophila embryos.

The following SPARC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPARC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06244	NM_001276069.1 Latest version!	Drosophila melanogaster secreted protein, acidic, cysteine-rich, transcript variant B (SPARC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa06244	NM_143252.4 Latest version!	Drosophila melanogaster secreted protein, acidic, cysteine-rich, transcript variant A (SPARC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPARC ( NM_143252.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651509.2 Drosophila melanogaster secreted protein, acidic, cysteine-rich, transcript variant A (SPARC), mRNA.

Price & Availability↑

CloneID	OFa06244
Clone ID Related Accession (Same CDS sequence)	NM_143252.4 , NM_001276069.1
Accession Version	NM_143252.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secreted protein, acidic, cysteine-rich, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143252.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCTCCC TTTGGCTGCT GCTCGGCTTG GGCCTGCTGG CTGTGAGCCA CGTCCAGGCC 
TCTACGGAGT TTTCCGAAGA TCTGCTGGAT GAGGACCTCG ACCTGTCCGA CATCGATGAG 
AACGAAGAGG AGTTCCTGCG CCTGCTGGAG GAGAAGAACA AGATCAAGGA TATTGAGCGC 
GAGAATGAGA TTGCCACCAA GCTGGCCGAA GTGCAGCACA ATCTACTCAA TCCCGTTGTC 
GAGGTGGATC TGTGCGAAAC GATGAGCTGC GGAGCCGGTC GCATCTGCCA GATGCACGAC 
GAGAAGCCCA AATGCGTGTG CATTCCGGAG TGCCCGGAGG AGGTGGACAC TCGCCGCCTG 
GTCTGCACCA ATACCAACGA GACCTGGCCG TCGGACTGCT CTGTGTATCA GCAGCGCTGC 
TGGTGCGACA GCGGCGAGCC CGGCTGCACC AATCCGGACA ACGCGCACAT GCACATCGAC 
TACTACGGCG CTTGCCACGA GCCCAGGAGC TGCGAGGGCG AGGACCTGAA GGACTTCCCC 
AGGCGCATGC GCGACTGGCT GTTCAACGTG ATGCGCGACC TGGCCGAGCG CGACGAGCTG 
ACCGAGCACT ACATGCAGAT GGAGCTGGAG GCGGAGACCA ACAACTCGCG TCGCTGGTCG 
AACGCCGCCG TGTGGAAGTG GTGCGACCTG GACGGCGATA CCGATCGCTC CGTCTCGCGC 
CACGAGCTCT TCCCCATCCG TGCTCCGCTG GTCAGTCTCG AGCACTGCAT CGCACCCTTC 
CTCGAGTCCT GCGACTCCAA CAAGGACCAT CGCATCACCC TGGTGGAGTG GGGCGCCTGC 
CTGGAGCTGG ATCCCGAGGA CCTCAAGGAG CGTTGCGACG ACGTCCAGCG GGCTCAGCCC 
CATCTTCTGG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651509.2
CDS	89..1003
Translation	MRSLWLLLGLGLLAVSHVQASTEFSEDLLDEDLDLSDIDENEEEFLRLLEEKNKIKDIERENEIATKLAEVQHNLLNPVVEVDLCETMSCGAGRICQMHDEKPKCVCIPECPEEVDTRRLVCTNTNETWPSDCSVYQQRCWCDSGEPGCTNPDNAHMHIDYYGACHEPRSCEGEDLKDFPRRMRDWLFNVMRDLAERDELTEHYMQMELEAETNNSRRWSNAAVWKWCDLDGDTDRSVSRHELFPIRAPLVSLEHCIAPFLESCDSNKDHRITLVEWGACLELDPEDLKERCDDVQRAQPHLLG

Target ORF information:

RefSeq Version	NM_143252.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secreted protein, acidic, cysteine-rich, transcript variant A (SPARC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143252.4

ORF Insert Sequence:

ATGCGCTCCC TTTGGCTGCT GCTCGGCTTG GGCCTGCTGG CTGTGAGCCA CGTCCAGGCC 
TCTACGGAGT TTTCCGAAGA TCTGCTGGAT GAGGACCTCG ACCTGTCCGA CATCGATGAG 
AACGAAGAGG AGTTCCTGCG CCTGCTGGAG GAGAAGAACA AGATCAAGGA TATTGAGCGC 
GAGAATGAGA TTGCCACCAA GCTGGCCGAA GTGCAGCACA ATCTACTCAA TCCCGTTGTC 
GAGGTGGATC TGTGCGAAAC GATGAGCTGC GGAGCCGGTC GCATCTGCCA GATGCACGAC 
GAGAAGCCCA AATGCGTGTG CATTCCGGAG TGCCCGGAGG AGGTGGACAC TCGCCGCCTG 
GTCTGCACCA ATACCAACGA GACCTGGCCG TCGGACTGCT CTGTGTATCA GCAGCGCTGC 
TGGTGCGACA GCGGCGAGCC CGGCTGCACC AATCCGGACA ACGCGCACAT GCACATCGAC 
TACTACGGCG CTTGCCACGA GCCCAGGAGC TGCGAGGGCG AGGACCTGAA GGACTTCCCC 
AGGCGCATGC GCGACTGGCT GTTCAACGTG ATGCGCGACC TGGCCGAGCG CGACGAGCTG 
ACCGAGCACT ACATGCAGAT GGAGCTGGAG GCGGAGACCA ACAACTCGCG TCGCTGGTCG 
AACGCCGCCG TGTGGAAGTG GTGCGACCTG GACGGCGATA CCGATCGCTC CGTCTCGCGC 
CACGAGCTCT TCCCCATCCG TGCTCCGCTG GTCAGTCTCG AGCACTGCAT CGCACCCTTC 
CTCGAGTCCT GCGACTCCAA CAAGGACCAT CGCATCACCC TGGTGGAGTG GGGCGCCTGC 
CTGGAGCTGG ATCCCGAGGA CCTCAAGGAG CGTTGCGACG ACGTCCAGCG GGCTCAGCCC 
CATCTTCTGG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPARC ( NM_001276069.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262998.1 Drosophila melanogaster secreted protein, acidic, cysteine-rich, transcript variant B (SPARC), mRNA.

Price & Availability↑

CloneID	OFa06244
Clone ID Related Accession (Same CDS sequence)	NM_143252.4 , NM_001276069.1
Accession Version	NM_001276069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secreted protein, acidic, cysteine-rich, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCTCCC TTTGGCTGCT GCTCGGCTTG GGCCTGCTGG CTGTGAGCCA CGTCCAGGCC 
TCTACGGAGT TTTCCGAAGA TCTGCTGGAT GAGGACCTCG ACCTGTCCGA CATCGATGAG 
AACGAAGAGG AGTTCCTGCG CCTGCTGGAG GAGAAGAACA AGATCAAGGA TATTGAGCGC 
GAGAATGAGA TTGCCACCAA GCTGGCCGAA GTGCAGCACA ATCTACTCAA TCCCGTTGTC 
GAGGTGGATC TGTGCGAAAC GATGAGCTGC GGAGCCGGTC GCATCTGCCA GATGCACGAC 
GAGAAGCCCA AATGCGTGTG CATTCCGGAG TGCCCGGAGG AGGTGGACAC TCGCCGCCTG 
GTCTGCACCA ATACCAACGA GACCTGGCCG TCGGACTGCT CTGTGTATCA GCAGCGCTGC 
TGGTGCGACA GCGGCGAGCC CGGCTGCACC AATCCGGACA ACGCGCACAT GCACATCGAC 
TACTACGGCG CTTGCCACGA GCCCAGGAGC TGCGAGGGCG AGGACCTGAA GGACTTCCCC 
AGGCGCATGC GCGACTGGCT GTTCAACGTG ATGCGCGACC TGGCCGAGCG CGACGAGCTG 
ACCGAGCACT ACATGCAGAT GGAGCTGGAG GCGGAGACCA ACAACTCGCG TCGCTGGTCG 
AACGCCGCCG TGTGGAAGTG GTGCGACCTG GACGGCGATA CCGATCGCTC CGTCTCGCGC 
CACGAGCTCT TCCCCATCCG TGCTCCGCTG GTCAGTCTCG AGCACTGCAT CGCACCCTTC 
CTCGAGTCCT GCGACTCCAA CAAGGACCAT CGCATCACCC TGGTGGAGTG GGGCGCCTGC 
CTGGAGCTGG ATCCCGAGGA CCTCAAGGAG CGTTGCGACG ACGTCCAGCG GGCTCAGCCC 
CATCTTCTGG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262998.1
CDS	77..991
Translation	MRSLWLLLGLGLLAVSHVQASTEFSEDLLDEDLDLSDIDENEEEFLRLLEEKNKIKDIERENEIATKLAEVQHNLLNPVVEVDLCETMSCGAGRICQMHDEKPKCVCIPECPEEVDTRRLVCTNTNETWPSDCSVYQQRCWCDSGEPGCTNPDNAHMHIDYYGACHEPRSCEGEDLKDFPRRMRDWLFNVMRDLAERDELTEHYMQMELEAETNNSRRWSNAAVWKWCDLDGDTDRSVSRHELFPIRAPLVSLEHCIAPFLESCDSNKDHRITLVEWGACLELDPEDLKERCDDVQRAQPHLLG

Target ORF information:

RefSeq Version	NM_001276069.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secreted protein, acidic, cysteine-rich, transcript variant B (SPARC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276069.1

ORF Insert Sequence:

ATGCGCTCCC TTTGGCTGCT GCTCGGCTTG GGCCTGCTGG CTGTGAGCCA CGTCCAGGCC 
TCTACGGAGT TTTCCGAAGA TCTGCTGGAT GAGGACCTCG ACCTGTCCGA CATCGATGAG 
AACGAAGAGG AGTTCCTGCG CCTGCTGGAG GAGAAGAACA AGATCAAGGA TATTGAGCGC 
GAGAATGAGA TTGCCACCAA GCTGGCCGAA GTGCAGCACA ATCTACTCAA TCCCGTTGTC 
GAGGTGGATC TGTGCGAAAC GATGAGCTGC GGAGCCGGTC GCATCTGCCA GATGCACGAC 
GAGAAGCCCA AATGCGTGTG CATTCCGGAG TGCCCGGAGG AGGTGGACAC TCGCCGCCTG 
GTCTGCACCA ATACCAACGA GACCTGGCCG TCGGACTGCT CTGTGTATCA GCAGCGCTGC 
TGGTGCGACA GCGGCGAGCC CGGCTGCACC AATCCGGACA ACGCGCACAT GCACATCGAC 
TACTACGGCG CTTGCCACGA GCCCAGGAGC TGCGAGGGCG AGGACCTGAA GGACTTCCCC 
AGGCGCATGC GCGACTGGCT GTTCAACGTG ATGCGCGACC TGGCCGAGCG CGACGAGCTG 
ACCGAGCACT ACATGCAGAT GGAGCTGGAG GCGGAGACCA ACAACTCGCG TCGCTGGTCG 
AACGCCGCCG TGTGGAAGTG GTGCGACCTG GACGGCGATA CCGATCGCTC CGTCTCGCGC 
CACGAGCTCT TCCCCATCCG TGCTCCGCTG GTCAGTCTCG AGCACTGCAT CGCACCCTTC 
CTCGAGTCCT GCGACTCCAA CAAGGACCAT CGCATCACCC TGGTGGAGTG GGGCGCCTGC 
CTGGAGCTGG ATCCCGAGGA CCTCAAGGAG CGTTGCGACG ACGTCCAGCG GGCTCAGCCC 
CATCTTCTGG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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SPARC cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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