CG6420 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG6420
Entrez Gene ID	43218
Full Name	CG6420 gene product from transcript CG6420-RB
Synonyms	Dmel\CG6420
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 97C5-97C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_143242.3	NP_651499.2	uncharacterized protein, isoform A
NM_001300616.1	NP_001287545.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	WDR20	XP_001163392.1
Canis lupus familiaris (dog)	WDR20	XP_005623993.1
Danio rerio (zebrafish)	si:dkey-241l7.1	XP_003200333.1
Homo sapiens (human)	WDR20	NP_851808.1
	WDR20	XP_001107352.1
Bos taurus (cattle)	WDR20	NP_001178085.1
Drosophila melanogaster (fruit fly)	CG6420	NP_651499.2
Xenopus tropicalis (tropical clawed frog)	wdr20	NP_001011447.1
Mus musculus (house mouse)	Wdr20	NP_081425.2
Rattus norvegicus (Norway rat)	Wdr20	NP_001094364.1
Gallus gallus (chicken)	WDR20	XP_003641482.2
Caenorhabditis elegans (roundworm)	hpo-22	NP_001256183.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG6420 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG6420 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06234	NM_143242.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG6420), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06234	NM_001300616.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG6420), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG6420 ( NM_143242.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651499.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG6420), mRNA.

Price & Availability↑

CloneID	OFa06234
Clone ID Related Accession (Same CDS sequence)	NM_143242.3 , NM_001300616.1
Accession Version	NM_143242.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143242.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACC AACTGGACGC CAGTGTGAAG GACGACCTGA AGACGCAGTT CGTCACCCGC 
GAGGGCACCT ACCGCCTGCT GACCCTCTCG GAATACTCGC GGCCCAACCG GGTGGGCTAC 
AGCAGCAACC AGAGCTCGCC GCAGGTGCGC GTCTCCATGG TGACCCTGCC CAGTCCGGCG 
CAGGGCAAGC TCGGCTCGGA TGTGGGCGTG GGCACACCTG TGGGCGGCGG AACAGCAGGA 
GCCAACACCA CCACAACGAA TGGCAGCTCG CCGGGAGCGT CGCCTACGGG AGCAGCCGGA 
GCCAGCACAG CAATATCGAA TGGAGGCGCT GGCGGTGACT ATAGCCACAG CAACCACAAC 
AGCAACAGTG CCGGAAACAA CACTGTGGAG GCGCGTCTGG GCGGCGGCAT ATCCATGCAC 
TCCATGATGA ACGGCGGCGT CGTCGACCAG AATGGCGTGG CCACCAATCA GGTCCTAGGA 
GGCGATCGCA TATGCTTCAA CTTCGGACGA GATCTCTACG TTTACTCCTT TCGCGGAGCC 
AAGAAGGGCA CGGAGATGAG CAAGCCGATC GATAAGAAGT TCTACAAGGG CACCAATCCC 
AGCTGCCACG ACTTCAACAT AAGCTCGGCC ACGCCCACGG GAGCGCCCCT TCTGGTGGGC 
TTCACCACAG GTCAAATCCA GCTGGTCTCC CCGCACGTTG GTCCTCGGGA AGTGAGGAAG 
CTCTTCAACG AGGAGCGACT GATCGACAAG ACCAAAGTGA CCTGCCTAAA GTGGCTACCC 
AACTCACCGC ACCTGTTCCT CGCCGCCCAC GCCTCAGGTC ACCTGTACCT GTACAACGAG 
GAACTACCCT GCGCCGCCAC AGCGCCCAGC TATCAGCCCT TCAAGCTTGG CGACGGCTAC 
ACGATACTCA CCAGCAAGTC CAAGACCACA AGGAACCCGC TCTTCAAGTG GGTCTTCAGC 
ACCGACAACT GCTGCGTCAA TGAGTTCTGC TTCTCGCCTT GCGGATCTCA CTTGGCGGTG 
GTTTCCCAGG ACGGTTTCCT GCGCGTCTTT CACTACGACA CCATGGAGCT GCTCGGAATC 
GCACGCTCCT ACTTTGGCGG CTTCCTCTGC GTGTGCTGGT CCCCTGACGG CAAGTACATT 
GTCGTGGGTG GCGAGGATGA CCTGGTGACT GTGTGGTCGC TCCACGAGCG ACGTGTAGTG 
GCTCGCGGAC AGGGCCATCG GTCCTGGGTC TCTGTGGTAG CGTTTGACCC GTATACCACC 
TCGTACACAA ACTGGGATGG CGGCGACTTC AGCGACGATG AGAATCAAAT GAACGAGTAC 
TCCCATTCGC GAGAGGCGCG CTTCTCCGGA GATTCCACGG CCAACGGTGG CTTCGAGGGT 
TTCGACCGAA ACTCCACACC CGTTCACGCT GACCGCAACG GACCACACTC GGCCTCCTTT 
CGCTCGGACG CTTCTTCGGC CGAAAAGCTA ATGAGTTATA GACTTGGCTC CGTCAGTCAG 
GATACCCAGA TCTGCCTATG GGACATCACG GAGGACGTTC TACGCCACCC ATTGGTGTTA 
AGGCAGCCCG CTAACAGTGA AAGGGCCTAT CTCAACGGTG GTGTCGACGA AGAAGCGGAA 
GCAGAAGACG GCATAAAGGT AATCCGACCA GTGGCCATGT CTGGTCAAGC GACTGGACAG 
CAAGCGGAAT CGGGCAGCTG TAGTCCAACA CGGGAAGCAG CGGGCGGAGG CACAGGGAAC 
GGTGCTGGCG AGCACAGCAA TAGCAGTTCC AGCAAGTTCT CAACCGCCAA CTGCACGATA 
TCCTCGCAAT CCTCGCCCGA CGACTGCGAC ACCGAGGCAG CCACTCCCGC CTCAACTTCA 
TCGAATGCGG GCGCAGGCTC GGTGGCTGGA GCGGGAGCGG TGGCCTCAAC GAAGCAGAAC 
AACCGCAACC ATGGCACTGG CAACTCAATC AAATTCCCCA ACTGCATCAG CGCCACCAAG 
TCAGACAGCA TTGACGGAGG CGGCGGCAGC GGGAGTGGAC AGAGGCCGTC GCAGACTACA 
TCGGGATACA ACAGCAAGAC TTCCAACAGC TCGAACAAGT CCTCCAACTC GGGCAGCGGC 
TTCAGCGCCT TCAACAGCCT CACGCAGCGT CTCTCGAACT TCAGTTTCCT GAGCAGCTCG 
GAAAAGAAGG GCGCTGGCTA TGAGGGAAGC CATTCCACCG CGCACCGTCA GCACCGCAAG 
GCAATGAGCA TGCTGAAGAG CTACAACCAA CACAACCACA CTGGCGGACA CAACAACCAC 
AGCCAGAGTA ACAACAGCAG CTCCTCCAAC TTTGGCCACT CGAGCACTCT GGATTCGAAT 
GCAGCCGGGG CCATTGGCAG CAGCTCGACG GCGCATAGTT TCGGATCACT GAAACTGAGC 
CGGTCCTCGC ACCACTCCTC ATTGGCCACA GCCGCCCATG CGTCGGGATC TGCAGCGGGC 
TCTGGCTCCG GTGTCAGCAG CTTCGATCCC ATGCAGCTTA TCGGAACTCC TGCCTGCCCC 
CGTTTCGATG AGTGCCCACT GCTGGAGCCG CTGGTCTGCA AGAAGATCGC CCACGAGCGG 
CTGACGGCGC TGATCTTTCG CGAGGACTGC TTCCTGACGG CGTGCCAGGA TGGATTCATC 
TACACCTGGG CGCGGCCAGG TCATGCCACG CACGCCACTC AGCACTTGTC ACCTGGACAG 
GCGGCGGCGC CCGGCGGGAC GGTAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651499.2
CDS	505..3234
Translation	MANQLDASVKDDLKTQFVTREGTYRLLTLSEYSRPNRVGYSSNQSSPQVRVSMVTLPSPAQGKLGSDVGVGTPVGGGTAGANTTTTNGSSPGASPTGAAGASTAISNGGAGGDYSHSNHNSNSAGNNTVEARLGGGISMHSMMNGGVVDQNGVATNQVLGGDRICFNFGRDLYVYSFRGAKKGTEMSKPIDKKFYKGTNPSCHDFNISSATPTGAPLLVGFTTGQIQLVSPHVGPREVRKLFNEERLIDKTKVTCLKWLPNSPHLFLAAHASGHLYLYNEELPCAATAPSYQPFKLGDGYTILTSKSKTTRNPLFKWVFSTDNCCVNEFCFSPCGSHLAVVSQDGFLRVFHYDTMELLGIARSYFGGFLCVCWSPDGKYIVVGGEDDLVTVWSLHERRVVARGQGHRSWVSVVAFDPYTTSYTNWDGGDFSDDENQMNEYSHSREARFSGDSTANGGFEGFDRNSTPVHADRNGPHSASFRSDASSAEKLMSYRLGSVSQDTQICLWDITEDVLRHPLVLRQPANSERAYLNGGVDEEAEAEDGIKVIRPVAMSGQATGQQAESGSCSPTREAAGGGTGNGAGEHSNSSSSKFSTANCTISSQSSPDDCDTEAATPASTSSNAGAGSVAGAGAVASTKQNNRNHGTGNSIKFPNCISATKSDSIDGGGGSGSGQRPSQTTSGYNSKTSNSSNKSSNSGSGFSAFNSLTQRLSNFSFLSSSEKKGAGYEGSHSTAHRQHRKAMSMLKSYNQHNHTGGHNNHSQSNNSSSSNFGHSSTLDSNAAGAIGSSSTAHSFGSLKLSRSSHHSSLATAAHASGSAAGSGSGVSSFDPMQLIGTPACPRFDECPLLEPLVCKKIAHERLTALIFREDCFLTACQDGFIYTWARPGHATHATQHLSPGQAAAPGGTVI

Target ORF information:

RefSeq Version	NM_143242.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG6420), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143242.3

ORF Insert Sequence:

ATGGCCAACC AACTGGACGC CAGTGTGAAG GACGACCTGA AGACGCAGTT CGTCACCCGC 
GAGGGCACCT ACCGCCTGCT GACCCTCTCG GAATACTCGC GGCCCAACCG GGTGGGCTAC 
AGCAGCAACC AGAGCTCGCC GCAGGTGCGC GTCTCCATGG TGACCCTGCC CAGTCCGGCG 
CAGGGCAAGC TCGGCTCGGA TGTGGGCGTG GGCACACCTG TGGGCGGCGG AACAGCAGGA 
GCCAACACCA CCACAACGAA TGGCAGCTCG CCGGGAGCGT CGCCTACGGG AGCAGCCGGA 
GCCAGCACAG CAATATCGAA TGGAGGCGCT GGCGGTGACT ATAGCCACAG CAACCACAAC 
AGCAACAGTG CCGGAAACAA CACTGTGGAG GCGCGTCTGG GCGGCGGCAT ATCCATGCAC 
TCCATGATGA ACGGCGGCGT CGTCGACCAG AATGGCGTGG CCACCAATCA GGTCCTAGGA 
GGCGATCGCA TATGCTTCAA CTTCGGACGA GATCTCTACG TTTACTCCTT TCGCGGAGCC 
AAGAAGGGCA CGGAGATGAG CAAGCCGATC GATAAGAAGT TCTACAAGGG CACCAATCCC 
AGCTGCCACG ACTTCAACAT AAGCTCGGCC ACGCCCACGG GAGCGCCCCT TCTGGTGGGC 
TTCACCACAG GTCAAATCCA GCTGGTCTCC CCGCACGTTG GTCCTCGGGA AGTGAGGAAG 
CTCTTCAACG AGGAGCGACT GATCGACAAG ACCAAAGTGA CCTGCCTAAA GTGGCTACCC 
AACTCACCGC ACCTGTTCCT CGCCGCCCAC GCCTCAGGTC ACCTGTACCT GTACAACGAG 
GAACTACCCT GCGCCGCCAC AGCGCCCAGC TATCAGCCCT TCAAGCTTGG CGACGGCTAC 
ACGATACTCA CCAGCAAGTC CAAGACCACA AGGAACCCGC TCTTCAAGTG GGTCTTCAGC 
ACCGACAACT GCTGCGTCAA TGAGTTCTGC TTCTCGCCTT GCGGATCTCA CTTGGCGGTG 
GTTTCCCAGG ACGGTTTCCT GCGCGTCTTT CACTACGACA CCATGGAGCT GCTCGGAATC 
GCACGCTCCT ACTTTGGCGG CTTCCTCTGC GTGTGCTGGT CCCCTGACGG CAAGTACATT 
GTCGTGGGTG GCGAGGATGA CCTGGTGACT GTGTGGTCGC TCCACGAGCG ACGTGTAGTG 
GCTCGCGGAC AGGGCCATCG GTCCTGGGTC TCTGTGGTAG CGTTTGACCC GTATACCACC 
TCGTACACAA ACTGGGATGG CGGCGACTTC AGCGACGATG AGAATCAAAT GAACGAGTAC 
TCCCATTCGC GAGAGGCGCG CTTCTCCGGA GATTCCACGG CCAACGGTGG CTTCGAGGGT 
TTCGACCGAA ACTCCACACC CGTTCACGCT GACCGCAACG GACCACACTC GGCCTCCTTT 
CGCTCGGACG CTTCTTCGGC CGAAAAGCTA ATGAGTTATA GACTTGGCTC CGTCAGTCAG 
GATACCCAGA TCTGCCTATG GGACATCACG GAGGACGTTC TACGCCACCC ATTGGTGTTA 
AGGCAGCCCG CTAACAGTGA AAGGGCCTAT CTCAACGGTG GTGTCGACGA AGAAGCGGAA 
GCAGAAGACG GCATAAAGGT AATCCGACCA GTGGCCATGT CTGGTCAAGC GACTGGACAG 
CAAGCGGAAT CGGGCAGCTG TAGTCCAACA CGGGAAGCAG CGGGCGGAGG CACAGGGAAC 
GGTGCTGGCG AGCACAGCAA TAGCAGTTCC AGCAAGTTCT CAACCGCCAA CTGCACGATA 
TCCTCGCAAT CCTCGCCCGA CGACTGCGAC ACCGAGGCAG CCACTCCCGC CTCAACTTCA 
TCGAATGCGG GCGCAGGCTC GGTGGCTGGA GCGGGAGCGG TGGCCTCAAC GAAGCAGAAC 
AACCGCAACC ATGGCACTGG CAACTCAATC AAATTCCCCA ACTGCATCAG CGCCACCAAG 
TCAGACAGCA TTGACGGAGG CGGCGGCAGC GGGAGTGGAC AGAGGCCGTC GCAGACTACA 
TCGGGATACA ACAGCAAGAC TTCCAACAGC TCGAACAAGT CCTCCAACTC GGGCAGCGGC 
TTCAGCGCCT TCAACAGCCT CACGCAGCGT CTCTCGAACT TCAGTTTCCT GAGCAGCTCG 
GAAAAGAAGG GCGCTGGCTA TGAGGGAAGC CATTCCACCG CGCACCGTCA GCACCGCAAG 
GCAATGAGCA TGCTGAAGAG CTACAACCAA CACAACCACA CTGGCGGACA CAACAACCAC 
AGCCAGAGTA ACAACAGCAG CTCCTCCAAC TTTGGCCACT CGAGCACTCT GGATTCGAAT 
GCAGCCGGGG CCATTGGCAG CAGCTCGACG GCGCATAGTT TCGGATCACT GAAACTGAGC 
CGGTCCTCGC ACCACTCCTC ATTGGCCACA GCCGCCCATG CGTCGGGATC TGCAGCGGGC 
TCTGGCTCCG GTGTCAGCAG CTTCGATCCC ATGCAGCTTA TCGGAACTCC TGCCTGCCCC 
CGTTTCGATG AGTGCCCACT GCTGGAGCCG CTGGTCTGCA AGAAGATCGC CCACGAGCGG 
CTGACGGCGC TGATCTTTCG CGAGGACTGC TTCCTGACGG CGTGCCAGGA TGGATTCATC 
TACACCTGGG CGCGGCCAGG TCATGCCACG CACGCCACTC AGCACTTGTC ACCTGGACAG 
GCGGCGGCGC CCGGCGGGAC GGTAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG6420 ( NM_001300616.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287545.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG6420), mRNA.

Price & Availability↑

CloneID	OFa06234
Clone ID Related Accession (Same CDS sequence)	NM_143242.3 , NM_001300616.1
Accession Version	NM_001300616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACC AACTGGACGC CAGTGTGAAG GACGACCTGA AGACGCAGTT CGTCACCCGC 
GAGGGCACCT ACCGCCTGCT GACCCTCTCG GAATACTCGC GGCCCAACCG GGTGGGCTAC 
AGCAGCAACC AGAGCTCGCC GCAGGTGCGC GTCTCCATGG TGACCCTGCC CAGTCCGGCG 
CAGGGCAAGC TCGGCTCGGA TGTGGGCGTG GGCACACCTG TGGGCGGCGG AACAGCAGGA 
GCCAACACCA CCACAACGAA TGGCAGCTCG CCGGGAGCGT CGCCTACGGG AGCAGCCGGA 
GCCAGCACAG CAATATCGAA TGGAGGCGCT GGCGGTGACT ATAGCCACAG CAACCACAAC 
AGCAACAGTG CCGGAAACAA CACTGTGGAG GCGCGTCTGG GCGGCGGCAT ATCCATGCAC 
TCCATGATGA ACGGCGGCGT CGTCGACCAG AATGGCGTGG CCACCAATCA GGTCCTAGGA 
GGCGATCGCA TATGCTTCAA CTTCGGACGA GATCTCTACG TTTACTCCTT TCGCGGAGCC 
AAGAAGGGCA CGGAGATGAG CAAGCCGATC GATAAGAAGT TCTACAAGGG CACCAATCCC 
AGCTGCCACG ACTTCAACAT AAGCTCGGCC ACGCCCACGG GAGCGCCCCT TCTGGTGGGC 
TTCACCACAG GTCAAATCCA GCTGGTCTCC CCGCACGTTG GTCCTCGGGA AGTGAGGAAG 
CTCTTCAACG AGGAGCGACT GATCGACAAG ACCAAAGTGA CCTGCCTAAA GTGGCTACCC 
AACTCACCGC ACCTGTTCCT CGCCGCCCAC GCCTCAGGTC ACCTGTACCT GTACAACGAG 
GAACTACCCT GCGCCGCCAC AGCGCCCAGC TATCAGCCCT TCAAGCTTGG CGACGGCTAC 
ACGATACTCA CCAGCAAGTC CAAGACCACA AGGAACCCGC TCTTCAAGTG GGTCTTCAGC 
ACCGACAACT GCTGCGTCAA TGAGTTCTGC TTCTCGCCTT GCGGATCTCA CTTGGCGGTG 
GTTTCCCAGG ACGGTTTCCT GCGCGTCTTT CACTACGACA CCATGGAGCT GCTCGGAATC 
GCACGCTCCT ACTTTGGCGG CTTCCTCTGC GTGTGCTGGT CCCCTGACGG CAAGTACATT 
GTCGTGGGTG GCGAGGATGA CCTGGTGACT GTGTGGTCGC TCCACGAGCG ACGTGTAGTG 
GCTCGCGGAC AGGGCCATCG GTCCTGGGTC TCTGTGGTAG CGTTTGACCC GTATACCACC 
TCGTACACAA ACTGGGATGG CGGCGACTTC AGCGACGATG AGAATCAAAT GAACGAGTAC 
TCCCATTCGC GAGAGGCGCG CTTCTCCGGA GATTCCACGG CCAACGGTGG CTTCGAGGGT 
TTCGACCGAA ACTCCACACC CGTTCACGCT GACCGCAACG GACCACACTC GGCCTCCTTT 
CGCTCGGACG CTTCTTCGGC CGAAAAGCTA ATGAGTTATA GACTTGGCTC CGTCAGTCAG 
GATACCCAGA TCTGCCTATG GGACATCACG GAGGACGTTC TACGCCACCC ATTGGTGTTA 
AGGCAGCCCG CTAACAGTGA AAGGGCCTAT CTCAACGGTG GTGTCGACGA AGAAGCGGAA 
GCAGAAGACG GCATAAAGGT AATCCGACCA GTGGCCATGT CTGGTCAAGC GACTGGACAG 
CAAGCGGAAT CGGGCAGCTG TAGTCCAACA CGGGAAGCAG CGGGCGGAGG CACAGGGAAC 
GGTGCTGGCG AGCACAGCAA TAGCAGTTCC AGCAAGTTCT CAACCGCCAA CTGCACGATA 
TCCTCGCAAT CCTCGCCCGA CGACTGCGAC ACCGAGGCAG CCACTCCCGC CTCAACTTCA 
TCGAATGCGG GCGCAGGCTC GGTGGCTGGA GCGGGAGCGG TGGCCTCAAC GAAGCAGAAC 
AACCGCAACC ATGGCACTGG CAACTCAATC AAATTCCCCA ACTGCATCAG CGCCACCAAG 
TCAGACAGCA TTGACGGAGG CGGCGGCAGC GGGAGTGGAC AGAGGCCGTC GCAGACTACA 
TCGGGATACA ACAGCAAGAC TTCCAACAGC TCGAACAAGT CCTCCAACTC GGGCAGCGGC 
TTCAGCGCCT TCAACAGCCT CACGCAGCGT CTCTCGAACT TCAGTTTCCT GAGCAGCTCG 
GAAAAGAAGG GCGCTGGCTA TGAGGGAAGC CATTCCACCG CGCACCGTCA GCACCGCAAG 
GCAATGAGCA TGCTGAAGAG CTACAACCAA CACAACCACA CTGGCGGACA CAACAACCAC 
AGCCAGAGTA ACAACAGCAG CTCCTCCAAC TTTGGCCACT CGAGCACTCT GGATTCGAAT 
GCAGCCGGGG CCATTGGCAG CAGCTCGACG GCGCATAGTT TCGGATCACT GAAACTGAGC 
CGGTCCTCGC ACCACTCCTC ATTGGCCACA GCCGCCCATG CGTCGGGATC TGCAGCGGGC 
TCTGGCTCCG GTGTCAGCAG CTTCGATCCC ATGCAGCTTA TCGGAACTCC TGCCTGCCCC 
CGTTTCGATG AGTGCCCACT GCTGGAGCCG CTGGTCTGCA AGAAGATCGC CCACGAGCGG 
CTGACGGCGC TGATCTTTCG CGAGGACTGC TTCCTGACGG CGTGCCAGGA TGGATTCATC 
TACACCTGGG CGCGGCCAGG TCATGCCACG CACGCCACTC AGCACTTGTC ACCTGGACAG 
GCGGCGGCGC CCGGCGGGAC GGTAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287545.1
CDS	505..3234
Translation	MANQLDASVKDDLKTQFVTREGTYRLLTLSEYSRPNRVGYSSNQSSPQVRVSMVTLPSPAQGKLGSDVGVGTPVGGGTAGANTTTTNGSSPGASPTGAAGASTAISNGGAGGDYSHSNHNSNSAGNNTVEARLGGGISMHSMMNGGVVDQNGVATNQVLGGDRICFNFGRDLYVYSFRGAKKGTEMSKPIDKKFYKGTNPSCHDFNISSATPTGAPLLVGFTTGQIQLVSPHVGPREVRKLFNEERLIDKTKVTCLKWLPNSPHLFLAAHASGHLYLYNEELPCAATAPSYQPFKLGDGYTILTSKSKTTRNPLFKWVFSTDNCCVNEFCFSPCGSHLAVVSQDGFLRVFHYDTMELLGIARSYFGGFLCVCWSPDGKYIVVGGEDDLVTVWSLHERRVVARGQGHRSWVSVVAFDPYTTSYTNWDGGDFSDDENQMNEYSHSREARFSGDSTANGGFEGFDRNSTPVHADRNGPHSASFRSDASSAEKLMSYRLGSVSQDTQICLWDITEDVLRHPLVLRQPANSERAYLNGGVDEEAEAEDGIKVIRPVAMSGQATGQQAESGSCSPTREAAGGGTGNGAGEHSNSSSSKFSTANCTISSQSSPDDCDTEAATPASTSSNAGAGSVAGAGAVASTKQNNRNHGTGNSIKFPNCISATKSDSIDGGGGSGSGQRPSQTTSGYNSKTSNSSNKSSNSGSGFSAFNSLTQRLSNFSFLSSSEKKGAGYEGSHSTAHRQHRKAMSMLKSYNQHNHTGGHNNHSQSNNSSSSNFGHSSTLDSNAAGAIGSSSTAHSFGSLKLSRSSHHSSLATAAHASGSAAGSGSGVSSFDPMQLIGTPACPRFDECPLLEPLVCKKIAHERLTALIFREDCFLTACQDGFIYTWARPGHATHATQHLSPGQAAAPGGTVI

Target ORF information:

RefSeq Version	NM_001300616.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG6420), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300616.1

ORF Insert Sequence:

ATGGCCAACC AACTGGACGC CAGTGTGAAG GACGACCTGA AGACGCAGTT CGTCACCCGC 
GAGGGCACCT ACCGCCTGCT GACCCTCTCG GAATACTCGC GGCCCAACCG GGTGGGCTAC 
AGCAGCAACC AGAGCTCGCC GCAGGTGCGC GTCTCCATGG TGACCCTGCC CAGTCCGGCG 
CAGGGCAAGC TCGGCTCGGA TGTGGGCGTG GGCACACCTG TGGGCGGCGG AACAGCAGGA 
GCCAACACCA CCACAACGAA TGGCAGCTCG CCGGGAGCGT CGCCTACGGG AGCAGCCGGA 
GCCAGCACAG CAATATCGAA TGGAGGCGCT GGCGGTGACT ATAGCCACAG CAACCACAAC 
AGCAACAGTG CCGGAAACAA CACTGTGGAG GCGCGTCTGG GCGGCGGCAT ATCCATGCAC 
TCCATGATGA ACGGCGGCGT CGTCGACCAG AATGGCGTGG CCACCAATCA GGTCCTAGGA 
GGCGATCGCA TATGCTTCAA CTTCGGACGA GATCTCTACG TTTACTCCTT TCGCGGAGCC 
AAGAAGGGCA CGGAGATGAG CAAGCCGATC GATAAGAAGT TCTACAAGGG CACCAATCCC 
AGCTGCCACG ACTTCAACAT AAGCTCGGCC ACGCCCACGG GAGCGCCCCT TCTGGTGGGC 
TTCACCACAG GTCAAATCCA GCTGGTCTCC CCGCACGTTG GTCCTCGGGA AGTGAGGAAG 
CTCTTCAACG AGGAGCGACT GATCGACAAG ACCAAAGTGA CCTGCCTAAA GTGGCTACCC 
AACTCACCGC ACCTGTTCCT CGCCGCCCAC GCCTCAGGTC ACCTGTACCT GTACAACGAG 
GAACTACCCT GCGCCGCCAC AGCGCCCAGC TATCAGCCCT TCAAGCTTGG CGACGGCTAC 
ACGATACTCA CCAGCAAGTC CAAGACCACA AGGAACCCGC TCTTCAAGTG GGTCTTCAGC 
ACCGACAACT GCTGCGTCAA TGAGTTCTGC TTCTCGCCTT GCGGATCTCA CTTGGCGGTG 
GTTTCCCAGG ACGGTTTCCT GCGCGTCTTT CACTACGACA CCATGGAGCT GCTCGGAATC 
GCACGCTCCT ACTTTGGCGG CTTCCTCTGC GTGTGCTGGT CCCCTGACGG CAAGTACATT 
GTCGTGGGTG GCGAGGATGA CCTGGTGACT GTGTGGTCGC TCCACGAGCG ACGTGTAGTG 
GCTCGCGGAC AGGGCCATCG GTCCTGGGTC TCTGTGGTAG CGTTTGACCC GTATACCACC 
TCGTACACAA ACTGGGATGG CGGCGACTTC AGCGACGATG AGAATCAAAT GAACGAGTAC 
TCCCATTCGC GAGAGGCGCG CTTCTCCGGA GATTCCACGG CCAACGGTGG CTTCGAGGGT 
TTCGACCGAA ACTCCACACC CGTTCACGCT GACCGCAACG GACCACACTC GGCCTCCTTT 
CGCTCGGACG CTTCTTCGGC CGAAAAGCTA ATGAGTTATA GACTTGGCTC CGTCAGTCAG 
GATACCCAGA TCTGCCTATG GGACATCACG GAGGACGTTC TACGCCACCC ATTGGTGTTA 
AGGCAGCCCG CTAACAGTGA AAGGGCCTAT CTCAACGGTG GTGTCGACGA AGAAGCGGAA 
GCAGAAGACG GCATAAAGGT AATCCGACCA GTGGCCATGT CTGGTCAAGC GACTGGACAG 
CAAGCGGAAT CGGGCAGCTG TAGTCCAACA CGGGAAGCAG CGGGCGGAGG CACAGGGAAC 
GGTGCTGGCG AGCACAGCAA TAGCAGTTCC AGCAAGTTCT CAACCGCCAA CTGCACGATA 
TCCTCGCAAT CCTCGCCCGA CGACTGCGAC ACCGAGGCAG CCACTCCCGC CTCAACTTCA 
TCGAATGCGG GCGCAGGCTC GGTGGCTGGA GCGGGAGCGG TGGCCTCAAC GAAGCAGAAC 
AACCGCAACC ATGGCACTGG CAACTCAATC AAATTCCCCA ACTGCATCAG CGCCACCAAG 
TCAGACAGCA TTGACGGAGG CGGCGGCAGC GGGAGTGGAC AGAGGCCGTC GCAGACTACA 
TCGGGATACA ACAGCAAGAC TTCCAACAGC TCGAACAAGT CCTCCAACTC GGGCAGCGGC 
TTCAGCGCCT TCAACAGCCT CACGCAGCGT CTCTCGAACT TCAGTTTCCT GAGCAGCTCG 
GAAAAGAAGG GCGCTGGCTA TGAGGGAAGC CATTCCACCG CGCACCGTCA GCACCGCAAG 
GCAATGAGCA TGCTGAAGAG CTACAACCAA CACAACCACA CTGGCGGACA CAACAACCAC 
AGCCAGAGTA ACAACAGCAG CTCCTCCAAC TTTGGCCACT CGAGCACTCT GGATTCGAAT 
GCAGCCGGGG CCATTGGCAG CAGCTCGACG GCGCATAGTT TCGGATCACT GAAACTGAGC 
CGGTCCTCGC ACCACTCCTC ATTGGCCACA GCCGCCCATG CGTCGGGATC TGCAGCGGGC 
TCTGGCTCCG GTGTCAGCAG CTTCGATCCC ATGCAGCTTA TCGGAACTCC TGCCTGCCCC 
CGTTTCGATG AGTGCCCACT GCTGGAGCCG CTGGTCTGCA AGAAGATCGC CCACGAGCGG 
CTGACGGCGC TGATCTTTCG CGAGGACTGC TTCCTGACGG CGTGCCAGGA TGGATTCATC 
TACACCTGGG CGCGGCCAGG TCATGCCACG CACGCCACTC AGCACTTGTC ACCTGGACAG 
GCGGCGGCGC CCGGCGGGAC GGTAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG6420 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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