Npl4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npl4
Entrez Gene ID	43091
Full Name	Nuclear protein localization 4
Synonyms	CG4673,Dmel\CG4673,NPL4,dNPLOC4,dNp14,dNpl4,npl4
General protein information	Preferred Names Nuclear protein localization 4 Names CG4673 gene product from transcript CG4673-RB CG4673-PA CG4673-PB CG4673-PD Npl4 ortholog Npl4-PA Npl4-PB Npl4-PD nuclear protein localization 4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96E4-96E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_170231.3	NP_733110.2	nuclear protein localization 4, isoform B
NM_143150.4	NP_651407.3	nuclear protein localization 4, isoform A
NM_001170260.2	NP_001163731.1	nuclear protein localization 4, isoform D

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	NPL4	NP_009729.1
Kluyveromyces lactis	KLLA0E24575g	XP_455058.1
Eremothecium gossypii	AGOS_AGR211W	NP_986877.1
Magnaporthe oryzae (rice blast fungus)	MGG_04413	XP_003713511.1
	LOC713927	XP_001111288.1
Rattus norvegicus (Norway rat)	Nploc4	NP_542144.1
Danio rerio (zebrafish)	nploc4	NP_956101.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001986	XP_550827.4
Xenopus tropicalis (tropical clawed frog)	nploc4	NP_001135712.1
Pan troglodytes (chimpanzee)	NPLOC4	XP_003315818.1
Canis lupus familiaris (dog)	NPLOC4	XP_540482.2
Drosophila melanogaster (fruit fly)	Npl4	NP_001163731.1
Homo sapiens (human)	NPLOC4	NP_060391.2
Mus musculus (house mouse)	Nploc4	NP_001181952.1
Gallus gallus (chicken)	NPLOC4	XP_415704.3
Caenorhabditis elegans (roundworm)	npl-4.2	NP_495093.1
Caenorhabditis elegans (roundworm)	npl-4.1	NP_495096.1
Bos taurus (cattle)	NPLOC4	NP_001179118.1
Schizosaccharomyces pombe (fission yeast)	npl4	NP_595883.2
Neurospora crassa	NCU02680	XP_965453.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Npl4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npl4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06109	NM_170231.3 Latest version!	Drosophila melanogaster nuclear protein localization 4, transcript variant B (Npl4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06109	NM_001170260.2 Latest version!	Drosophila melanogaster nuclear protein localization 4, transcript variant D (Npl4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06110	NM_143150.4 Latest version!	Drosophila melanogaster nuclear protein localization 4, transcript variant A (Npl4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npl4 ( NM_170231.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733110.2 Drosophila melanogaster nuclear protein localization 4, transcript variant B (Npl4), mRNA.

Price & Availability↑

CloneID	OFa06109
Clone ID Related Accession (Same CDS sequence)	NM_170231.3 , NM_001170260.2
Accession Version	NM_170231.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nuclear protein localization 4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170231.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAAATG ACAAAATTCT AATTCGCGTT CAGTCGGCGG AGGGCATCAA ACGCATCGAG 
ATCTCGCCCA AGTCAAATCT AAAGCATCTG TACGACAGCG TTCAGAATGC CCTGAAGGTG 
GATGGATTTG GTCTCTTCAA GGAGCGCAAC TTCCTCACAG AGCTGCAGGC AAGTGGCTCC 
CAGCTGGTGG GGACTTCCTT GAGGCATGGC GACATGGTCT ACTTAAAACA GATGGCTGGC 
ACATCATCGC GCCGCACTAG CACCACTGTC CTGGACAGTC AGGCGTTCAA GACTAGCACC 
ATTAGCAATC CAAACAGCGC TCGTCCATCC TTCAATGTGA TCGAAGATGA CGTGGACCAG 
GCTCTGAGCA AGGCTGATGG CACCATTAAG CGCGAACGTG ATAGCAAGCT GTGCCACCAC 
AATGCCAACG GCCGTTGCGT TCACTGCTCC GCACTGGAGC CCTATGATGA GTCCTATCTG 
AAGGAGCACA ATATCAAGCA CTTGTCCTTC CACTCGTACA TACGAAAGCA GACATCCGGC 
ATGGATCAGG GCAAGTACTT TGTCTTCGAC GACATCAATT GCCGCATCAA GCCGGGATGT 
CGCGAGCATC CACCATGGCC CAAGGGCATC TGCTCCAAGT GCCAGCCGTC GGCCATTACG 
CTGAATCGTC AGACATATCG CCACGTGGAC AACGTCATGT TCGAGAACAC TAAGATCGTG 
GAGCGCTTCC TCAACTACTG GCGCACCACA GGACACCAGC GCATGGGATA TCTGTATGGG 
ACGTACGAGC AGCACACTGA TGTTCCGTTG GGCATTCGGG CCAAGGTGGC TGCCATTTAT 
GAGCCGCCAC AAGAGTCTAC CAGGGATTCG ATCAATATTC AGCCGGATGA GTTCGCCGAC 
GATGTGGATG CCGTAGCCAG TGCGCTGGGA CTGAAGAAGA TTGGTTGGAT TTTCACTGAT 
CTCATCACGG ACGATGCGAG TATCGGCACT GTCAAACAGA TCCGTGGCAT CGAGTCGCAC 
TTTATTACGG CCCAGGAGTG CATCACAGCT GGCGAGCTGC AGAATCGACA TCCAAATCCG 
TGTAAATATG CCTCCAATGG CGTCTTCGGC TCTAAGTTTG TGACCATTTG TGTAACAGGA 
GATAAAACCA AACAAGTGCA CATGGAAGGC TATGCGGTTT CGGCTCAGTG CATGGCTCTG 
GTGCGCGATA ATTGTCTGAT ACCCACTAAG GATGCGCCCG AGCTGGGCTA CGTGCGTGAA 
TCCACGGACA AGCAGTATGT TCCCGACGTT TTCTATAAGG AGAAGGATCT GTACGGCAAC 
GAGGTGCAGC GGCTGGCTCG CCCCCTGCCC GTGGAGTATT TGCTGGTGGA CGTGCCCGCA 
TCCACACCGC TGCAACCCAT TTACACCTTC ACCGAGTACG ACAAGCGCCA GCCATTCCCC 
ATCGAGAACC GCTACATCGA TGGCCACCTG CAGGATTTCA ATGCACTCAG CTGCTATCTG 
TCCGCCTGGG GCGAGGAGGA GTTCCTGGAG GCCATCTCCG ATTTCCACCT GCTGGTCTAC 
CTGTACAAGA TGGACATGCT GCCGCTGCGA CAGCACATGG GTCCGCTGCT GGAGGCAGTT 
CGCACCAAGA ACCCCAACCA GGCCGCCCAG TTCAAGGTCG TGGATGTGTG GAAGCTGCTC 
GAGTCGCTTA TCCAGGCCAG TTCCGGCGGC AGTGGTGGAA CCACCAGCTA TCCCAGTGGT 
GGAGCTTCGG CCAGTGCCGG AGCTTCTGGA TCGGAGGCAA TGGACTTGGA CGCCAACACC 
TGGACATGCA ACCACTGCAC CTTCATCAAC CGCGGCGAGC TAACGTCCTG CGAGATCTGC 
TCGCTACCCA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733110.2
CDS	57..1931
Translation	MPNDKILIRVQSAEGIKRIEISPKSNLKHLYDSVQNALKVDGFGLFKERNFLTELQASGSQLVGTSLRHGDMVYLKQMAGTSSRRTSTTVLDSQAFKTSTISNPNSARPSFNVIEDDVDQALSKADGTIKRERDSKLCHHNANGRCVHCSALEPYDESYLKEHNIKHLSFHSYIRKQTSGMDQGKYFVFDDINCRIKPGCREHPPWPKGICSKCQPSAITLNRQTYRHVDNVMFENTKIVERFLNYWRTTGHQRMGYLYGTYEQHTDVPLGIRAKVAAIYEPPQESTRDSINIQPDEFADDVDAVASALGLKKIGWIFTDLITDDASIGTVKQIRGIESHFITAQECITAGELQNRHPNPCKYASNGVFGSKFVTICVTGDKTKQVHMEGYAVSAQCMALVRDNCLIPTKDAPELGYVRESTDKQYVPDVFYKEKDLYGNEVQRLARPLPVEYLLVDVPASTPLQPIYTFTEYDKRQPFPIENRYIDGHLQDFNALSCYLSAWGEEEFLEAISDFHLLVYLYKMDMLPLRQHMGPLLEAVRTKNPNQAAQFKVVDVWKLLESLIQASSGGSGGTTSYPSGGASASAGASGSEAMDLDANTWTCNHCTFINRGELTSCEICSLPR

Target ORF information:

RefSeq Version	NM_170231.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nuclear protein localization 4, transcript variant B (Npl4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170231.3

ORF Insert Sequence:

ATGCCAAATG ACAAAATTCT AATTCGCGTT CAGTCGGCGG AGGGCATCAA ACGCATCGAG 
ATCTCGCCCA AGTCAAATCT AAAGCATCTG TACGACAGCG TTCAGAATGC CCTGAAGGTG 
GATGGATTTG GTCTCTTCAA GGAGCGCAAC TTCCTCACAG AGCTGCAGGC AAGTGGCTCC 
CAGCTGGTGG GGACTTCCTT GAGGCATGGC GACATGGTCT ACTTAAAACA GATGGCTGGC 
ACATCATCGC GCCGCACTAG CACCACTGTC CTGGACAGTC AGGCGTTCAA GACTAGCACC 
ATTAGCAATC CAAACAGCGC TCGTCCATCC TTCAATGTGA TCGAAGATGA CGTGGACCAG 
GCTCTGAGCA AGGCTGATGG CACCATTAAG CGCGAACGTG ATAGCAAGCT GTGCCACCAC 
AATGCCAACG GCCGTTGCGT TCACTGCTCC GCACTGGAGC CCTATGATGA GTCCTATCTG 
AAGGAGCACA ATATCAAGCA CTTGTCCTTC CACTCGTACA TACGAAAGCA GACATCCGGC 
ATGGATCAGG GCAAGTACTT TGTCTTCGAC GACATCAATT GCCGCATCAA GCCGGGATGT 
CGCGAGCATC CACCATGGCC CAAGGGCATC TGCTCCAAGT GCCAGCCGTC GGCCATTACG 
CTGAATCGTC AGACATATCG CCACGTGGAC AACGTCATGT TCGAGAACAC TAAGATCGTG 
GAGCGCTTCC TCAACTACTG GCGCACCACA GGACACCAGC GCATGGGATA TCTGTATGGG 
ACGTACGAGC AGCACACTGA TGTTCCGTTG GGCATTCGGG CCAAGGTGGC TGCCATTTAT 
GAGCCGCCAC AAGAGTCTAC CAGGGATTCG ATCAATATTC AGCCGGATGA GTTCGCCGAC 
GATGTGGATG CCGTAGCCAG TGCGCTGGGA CTGAAGAAGA TTGGTTGGAT TTTCACTGAT 
CTCATCACGG ACGATGCGAG TATCGGCACT GTCAAACAGA TCCGTGGCAT CGAGTCGCAC 
TTTATTACGG CCCAGGAGTG CATCACAGCT GGCGAGCTGC AGAATCGACA TCCAAATCCG 
TGTAAATATG CCTCCAATGG CGTCTTCGGC TCTAAGTTTG TGACCATTTG TGTAACAGGA 
GATAAAACCA AACAAGTGCA CATGGAAGGC TATGCGGTTT CGGCTCAGTG CATGGCTCTG 
GTGCGCGATA ATTGTCTGAT ACCCACTAAG GATGCGCCCG AGCTGGGCTA CGTGCGTGAA 
TCCACGGACA AGCAGTATGT TCCCGACGTT TTCTATAAGG AGAAGGATCT GTACGGCAAC 
GAGGTGCAGC GGCTGGCTCG CCCCCTGCCC GTGGAGTATT TGCTGGTGGA CGTGCCCGCA 
TCCACACCGC TGCAACCCAT TTACACCTTC ACCGAGTACG ACAAGCGCCA GCCATTCCCC 
ATCGAGAACC GCTACATCGA TGGCCACCTG CAGGATTTCA ATGCACTCAG CTGCTATCTG 
TCCGCCTGGG GCGAGGAGGA GTTCCTGGAG GCCATCTCCG ATTTCCACCT GCTGGTCTAC 
CTGTACAAGA TGGACATGCT GCCGCTGCGA CAGCACATGG GTCCGCTGCT GGAGGCAGTT 
CGCACCAAGA ACCCCAACCA GGCCGCCCAG TTCAAGGTCG TGGATGTGTG GAAGCTGCTC 
GAGTCGCTTA TCCAGGCCAG TTCCGGCGGC AGTGGTGGAA CCACCAGCTA TCCCAGTGGT 
GGAGCTTCGG CCAGTGCCGG AGCTTCTGGA TCGGAGGCAA TGGACTTGGA CGCCAACACC 
TGGACATGCA ACCACTGCAC CTTCATCAAC CGCGGCGAGC TAACGTCCTG CGAGATCTGC 
TCGCTACCCA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npl4 ( NM_001170260.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163731.1 Drosophila melanogaster nuclear protein localization 4, transcript variant D (Npl4), mRNA.

Price & Availability↑

CloneID	OFa06109
Clone ID Related Accession (Same CDS sequence)	NM_170231.3 , NM_001170260.2
Accession Version	NM_001170260.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nuclear protein localization 4, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170260.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAAATG ACAAAATTCT AATTCGCGTT CAGTCGGCGG AGGGCATCAA ACGCATCGAG 
ATCTCGCCCA AGTCAAATCT AAAGCATCTG TACGACAGCG TTCAGAATGC CCTGAAGGTG 
GATGGATTTG GTCTCTTCAA GGAGCGCAAC TTCCTCACAG AGCTGCAGGC AAGTGGCTCC 
CAGCTGGTGG GGACTTCCTT GAGGCATGGC GACATGGTCT ACTTAAAACA GATGGCTGGC 
ACATCATCGC GCCGCACTAG CACCACTGTC CTGGACAGTC AGGCGTTCAA GACTAGCACC 
ATTAGCAATC CAAACAGCGC TCGTCCATCC TTCAATGTGA TCGAAGATGA CGTGGACCAG 
GCTCTGAGCA AGGCTGATGG CACCATTAAG CGCGAACGTG ATAGCAAGCT GTGCCACCAC 
AATGCCAACG GCCGTTGCGT TCACTGCTCC GCACTGGAGC CCTATGATGA GTCCTATCTG 
AAGGAGCACA ATATCAAGCA CTTGTCCTTC CACTCGTACA TACGAAAGCA GACATCCGGC 
ATGGATCAGG GCAAGTACTT TGTCTTCGAC GACATCAATT GCCGCATCAA GCCGGGATGT 
CGCGAGCATC CACCATGGCC CAAGGGCATC TGCTCCAAGT GCCAGCCGTC GGCCATTACG 
CTGAATCGTC AGACATATCG CCACGTGGAC AACGTCATGT TCGAGAACAC TAAGATCGTG 
GAGCGCTTCC TCAACTACTG GCGCACCACA GGACACCAGC GCATGGGATA TCTGTATGGG 
ACGTACGAGC AGCACACTGA TGTTCCGTTG GGCATTCGGG CCAAGGTGGC TGCCATTTAT 
GAGCCGCCAC AAGAGTCTAC CAGGGATTCG ATCAATATTC AGCCGGATGA GTTCGCCGAC 
GATGTGGATG CCGTAGCCAG TGCGCTGGGA CTGAAGAAGA TTGGTTGGAT TTTCACTGAT 
CTCATCACGG ACGATGCGAG TATCGGCACT GTCAAACAGA TCCGTGGCAT CGAGTCGCAC 
TTTATTACGG CCCAGGAGTG CATCACAGCT GGCGAGCTGC AGAATCGACA TCCAAATCCG 
TGTAAATATG CCTCCAATGG CGTCTTCGGC TCTAAGTTTG TGACCATTTG TGTAACAGGA 
GATAAAACCA AACAAGTGCA CATGGAAGGC TATGCGGTTT CGGCTCAGTG CATGGCTCTG 
GTGCGCGATA ATTGTCTGAT ACCCACTAAG GATGCGCCCG AGCTGGGCTA CGTGCGTGAA 
TCCACGGACA AGCAGTATGT TCCCGACGTT TTCTATAAGG AGAAGGATCT GTACGGCAAC 
GAGGTGCAGC GGCTGGCTCG CCCCCTGCCC GTGGAGTATT TGCTGGTGGA CGTGCCCGCA 
TCCACACCGC TGCAACCCAT TTACACCTTC ACCGAGTACG ACAAGCGCCA GCCATTCCCC 
ATCGAGAACC GCTACATCGA TGGCCACCTG CAGGATTTCA ATGCACTCAG CTGCTATCTG 
TCCGCCTGGG GCGAGGAGGA GTTCCTGGAG GCCATCTCCG ATTTCCACCT GCTGGTCTAC 
CTGTACAAGA TGGACATGCT GCCGCTGCGA CAGCACATGG GTCCGCTGCT GGAGGCAGTT 
CGCACCAAGA ACCCCAACCA GGCCGCCCAG TTCAAGGTCG TGGATGTGTG GAAGCTGCTC 
GAGTCGCTTA TCCAGGCCAG TTCCGGCGGC AGTGGTGGAA CCACCAGCTA TCCCAGTGGT 
GGAGCTTCGG CCAGTGCCGG AGCTTCTGGA TCGGAGGCAA TGGACTTGGA CGCCAACACC 
TGGACATGCA ACCACTGCAC CTTCATCAAC CGCGGCGAGC TAACGTCCTG CGAGATCTGC 
TCGCTACCCA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163731.1
CDS	57..1931
Translation	MPNDKILIRVQSAEGIKRIEISPKSNLKHLYDSVQNALKVDGFGLFKERNFLTELQASGSQLVGTSLRHGDMVYLKQMAGTSSRRTSTTVLDSQAFKTSTISNPNSARPSFNVIEDDVDQALSKADGTIKRERDSKLCHHNANGRCVHCSALEPYDESYLKEHNIKHLSFHSYIRKQTSGMDQGKYFVFDDINCRIKPGCREHPPWPKGICSKCQPSAITLNRQTYRHVDNVMFENTKIVERFLNYWRTTGHQRMGYLYGTYEQHTDVPLGIRAKVAAIYEPPQESTRDSINIQPDEFADDVDAVASALGLKKIGWIFTDLITDDASIGTVKQIRGIESHFITAQECITAGELQNRHPNPCKYASNGVFGSKFVTICVTGDKTKQVHMEGYAVSAQCMALVRDNCLIPTKDAPELGYVRESTDKQYVPDVFYKEKDLYGNEVQRLARPLPVEYLLVDVPASTPLQPIYTFTEYDKRQPFPIENRYIDGHLQDFNALSCYLSAWGEEEFLEAISDFHLLVYLYKMDMLPLRQHMGPLLEAVRTKNPNQAAQFKVVDVWKLLESLIQASSGGSGGTTSYPSGGASASAGASGSEAMDLDANTWTCNHCTFINRGELTSCEICSLPR

Target ORF information:

RefSeq Version	NM_001170260.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nuclear protein localization 4, transcript variant D (Npl4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170260.2

ORF Insert Sequence:

ATGCCAAATG ACAAAATTCT AATTCGCGTT CAGTCGGCGG AGGGCATCAA ACGCATCGAG 
ATCTCGCCCA AGTCAAATCT AAAGCATCTG TACGACAGCG TTCAGAATGC CCTGAAGGTG 
GATGGATTTG GTCTCTTCAA GGAGCGCAAC TTCCTCACAG AGCTGCAGGC AAGTGGCTCC 
CAGCTGGTGG GGACTTCCTT GAGGCATGGC GACATGGTCT ACTTAAAACA GATGGCTGGC 
ACATCATCGC GCCGCACTAG CACCACTGTC CTGGACAGTC AGGCGTTCAA GACTAGCACC 
ATTAGCAATC CAAACAGCGC TCGTCCATCC TTCAATGTGA TCGAAGATGA CGTGGACCAG 
GCTCTGAGCA AGGCTGATGG CACCATTAAG CGCGAACGTG ATAGCAAGCT GTGCCACCAC 
AATGCCAACG GCCGTTGCGT TCACTGCTCC GCACTGGAGC CCTATGATGA GTCCTATCTG 
AAGGAGCACA ATATCAAGCA CTTGTCCTTC CACTCGTACA TACGAAAGCA GACATCCGGC 
ATGGATCAGG GCAAGTACTT TGTCTTCGAC GACATCAATT GCCGCATCAA GCCGGGATGT 
CGCGAGCATC CACCATGGCC CAAGGGCATC TGCTCCAAGT GCCAGCCGTC GGCCATTACG 
CTGAATCGTC AGACATATCG CCACGTGGAC AACGTCATGT TCGAGAACAC TAAGATCGTG 
GAGCGCTTCC TCAACTACTG GCGCACCACA GGACACCAGC GCATGGGATA TCTGTATGGG 
ACGTACGAGC AGCACACTGA TGTTCCGTTG GGCATTCGGG CCAAGGTGGC TGCCATTTAT 
GAGCCGCCAC AAGAGTCTAC CAGGGATTCG ATCAATATTC AGCCGGATGA GTTCGCCGAC 
GATGTGGATG CCGTAGCCAG TGCGCTGGGA CTGAAGAAGA TTGGTTGGAT TTTCACTGAT 
CTCATCACGG ACGATGCGAG TATCGGCACT GTCAAACAGA TCCGTGGCAT CGAGTCGCAC 
TTTATTACGG CCCAGGAGTG CATCACAGCT GGCGAGCTGC AGAATCGACA TCCAAATCCG 
TGTAAATATG CCTCCAATGG CGTCTTCGGC TCTAAGTTTG TGACCATTTG TGTAACAGGA 
GATAAAACCA AACAAGTGCA CATGGAAGGC TATGCGGTTT CGGCTCAGTG CATGGCTCTG 
GTGCGCGATA ATTGTCTGAT ACCCACTAAG GATGCGCCCG AGCTGGGCTA CGTGCGTGAA 
TCCACGGACA AGCAGTATGT TCCCGACGTT TTCTATAAGG AGAAGGATCT GTACGGCAAC 
GAGGTGCAGC GGCTGGCTCG CCCCCTGCCC GTGGAGTATT TGCTGGTGGA CGTGCCCGCA 
TCCACACCGC TGCAACCCAT TTACACCTTC ACCGAGTACG ACAAGCGCCA GCCATTCCCC 
ATCGAGAACC GCTACATCGA TGGCCACCTG CAGGATTTCA ATGCACTCAG CTGCTATCTG 
TCCGCCTGGG GCGAGGAGGA GTTCCTGGAG GCCATCTCCG ATTTCCACCT GCTGGTCTAC 
CTGTACAAGA TGGACATGCT GCCGCTGCGA CAGCACATGG GTCCGCTGCT GGAGGCAGTT 
CGCACCAAGA ACCCCAACCA GGCCGCCCAG TTCAAGGTCG TGGATGTGTG GAAGCTGCTC 
GAGTCGCTTA TCCAGGCCAG TTCCGGCGGC AGTGGTGGAA CCACCAGCTA TCCCAGTGGT 
GGAGCTTCGG CCAGTGCCGG AGCTTCTGGA TCGGAGGCAA TGGACTTGGA CGCCAACACC 
TGGACATGCA ACCACTGCAC CTTCATCAAC CGCGGCGAGC TAACGTCCTG CGAGATCTGC 
TCGCTACCCA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npl4 ( NM_143150.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651407.3 Drosophila melanogaster nuclear protein localization 4, transcript variant A (Npl4), mRNA.

Price & Availability↑

CloneID	OFa06110
Clone ID Related Accession (Same CDS sequence)	NM_143150.4
Accession Version	NM_143150.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nuclear protein localization 4, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_143150.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTGTG CACCACTGCT GCTCGAGCAA TTCATCTACA AGAAGCGCAA TTTCGCCTAC 
GCCTTTGTGC GCCATCGTCT GTTTGTGTTA TGTAAACAAT CGCTAATTCG CGTTCAGTCG 
GCGGAGGGCA TCAAACGCAT CGAGATCTCG CCCAAGTCAA ATCTAAAGCA TCTGTACGAC 
AGCGTTCAGA ATGCCCTGAA GGTGGATGGA TTTGGTCTCT TCAAGGAGCG CAACTTCCTC 
ACAGAGCTGC AGGCAAGTGG CTCCCAGCTG GTGGGGACTT CCTTGAGGCA TGGCGACATG 
GTCTACTTAA AACAGATGGC TGGCACATCA TCGCGCCGCA CTAGCACCAC TGTCCTGGAC 
AGTCAGGCGT TCAAGACTAG CACCATTAGC AATCCAAACA GCGCTCGTCC ATCCTTCAAT 
GTGATCGAAG ATGACGTGGA CCAGGCTCTG AGCAAGGCTG ATGGCACCAT TAAGCGCGAA 
CGTGATAGCA AGCTGTGCCA CCACAATGCC AACGGCCGTT GCGTTCACTG CTCCGCACTG 
GAGCCCTATG ATGAGTCCTA TCTGAAGGAG CACAATATCA AGCACTTGTC CTTCCACTCG 
TACATACGAA AGCAGACATC CGGCATGGAT CAGGGCAAGT ACTTTGTCTT CGACGACATC 
AATTGCCGCA TCAAGCCGGG ATGTCGCGAG CATCCACCAT GGCCCAAGGG CATCTGCTCC 
AAGTGCCAGC CGTCGGCCAT TACGCTGAAT CGTCAGACAT ATCGCCACGT GGACAACGTC 
ATGTTCGAGA ACACTAAGAT CGTGGAGCGC TTCCTCAACT ACTGGCGCAC CACAGGACAC 
CAGCGCATGG GATATCTGTA TGGGACGTAC GAGCAGCACA CTGATGTTCC GTTGGGCATT 
CGGGCCAAGG TGGCTGCCAT TTATGAGCCG CCACAAGAGT CTACCAGGGA TTCGATCAAT 
ATTCAGCCGG ATGAGTTCGC CGACGATGTG GATGCCGTAG CCAGTGCGCT GGGACTGAAG 
AAGATTGGTT GGATTTTCAC TGATCTCATC ACGGACGATG CGAGTATCGG CACTGTCAAA 
CAGATCCGTG GCATCGAGTC GCACTTTATT ACGGCCCAGG AGTGCATCAC AGCTGGCGAG 
CTGCAGAATC GACATCCAAA TCCGTGTAAA TATGCCTCCA ATGGCGTCTT CGGCTCTAAG 
TTTGTGACCA TTTGTGTAAC AGGAGATAAA ACCAAACAAG TGCACATGGA AGGCTATGCG 
GTTTCGGCTC AGTGCATGGC TCTGGTGCGC GATAATTGTC TGATACCCAC TAAGGATGCG 
CCCGAGCTGG GCTACGTGCG TGAATCCACG GACAAGCAGT ATGTTCCCGA CGTTTTCTAT 
AAGGAGAAGG ATCTGTACGG CAACGAGGTG CAGCGGCTGG CTCGCCCCCT GCCCGTGGAG 
TATTTGCTGG TGGACGTGCC CGCATCCACA CCGCTGCAAC CCATTTACAC CTTCACCGAG 
TACGACAAGC GCCAGCCATT CCCCATCGAG AACCGCTACA TCGATGGCCA CCTGCAGGAT 
TTCAATGCAC TCAGCTGCTA TCTGTCCGCC TGGGGCGAGG AGGAGTTCCT GGAGGCCATC 
TCCGATTTCC ACCTGCTGGT CTACCTGTAC AAGATGGACA TGCTGCCGCT GCGACAGCAC 
ATGGGTCCGC TGCTGGAGGC AGTTCGCACC AAGAACCCCA ACCAGGCCGC CCAGTTCAAG 
GTCGTGGATG TGTGGAAGCT GCTCGAGTCG CTTATCCAGG CCAGTTCCGG CGGCAGTGGT 
GGAACCACCA GCTATCCCAG TGGTGGAGCT TCGGCCAGTG CCGGAGCTTC TGGATCGGAG 
GCAATGGACT TGGACGCCAA CACCTGGACA TGCAACCACT GCACCTTCAT CAACCGCGGC 
GAGCTAACGT CCTGCGAGAT CTGCTCGCTA CCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651407.3
CDS	167..2125
Translation	MACAPLLLEQFIYKKRNFAYAFVRHRLFVLCKQSLIRVQSAEGIKRIEISPKSNLKHLYDSVQNALKVDGFGLFKERNFLTELQASGSQLVGTSLRHGDMVYLKQMAGTSSRRTSTTVLDSQAFKTSTISNPNSARPSFNVIEDDVDQALSKADGTIKRERDSKLCHHNANGRCVHCSALEPYDESYLKEHNIKHLSFHSYIRKQTSGMDQGKYFVFDDINCRIKPGCREHPPWPKGICSKCQPSAITLNRQTYRHVDNVMFENTKIVERFLNYWRTTGHQRMGYLYGTYEQHTDVPLGIRAKVAAIYEPPQESTRDSINIQPDEFADDVDAVASALGLKKIGWIFTDLITDDASIGTVKQIRGIESHFITAQECITAGELQNRHPNPCKYASNGVFGSKFVTICVTGDKTKQVHMEGYAVSAQCMALVRDNCLIPTKDAPELGYVRESTDKQYVPDVFYKEKDLYGNEVQRLARPLPVEYLLVDVPASTPLQPIYTFTEYDKRQPFPIENRYIDGHLQDFNALSCYLSAWGEEEFLEAISDFHLLVYLYKMDMLPLRQHMGPLLEAVRTKNPNQAAQFKVVDVWKLLESLIQASSGGSGGTTSYPSGGASASAGASGSEAMDLDANTWTCNHCTFINRGELTSCEICSLPR

Target ORF information:

RefSeq Version	NM_143150.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nuclear protein localization 4, transcript variant A (Npl4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143150.4

ORF Insert Sequence:

ATGGCCTGTG CACCACTGCT GCTCGAGCAA TTCATCTACA AGAAGCGCAA TTTCGCCTAC 
GCCTTTGTGC GCCATCGTCT GTTTGTGTTA TGTAAACAAT CGCTAATTCG CGTTCAGTCG 
GCGGAGGGCA TCAAACGCAT CGAGATCTCG CCCAAGTCAA ATCTAAAGCA TCTGTACGAC 
AGCGTTCAGA ATGCCCTGAA GGTGGATGGA TTTGGTCTCT TCAAGGAGCG CAACTTCCTC 
ACAGAGCTGC AGGCAAGTGG CTCCCAGCTG GTGGGGACTT CCTTGAGGCA TGGCGACATG 
GTCTACTTAA AACAGATGGC TGGCACATCA TCGCGCCGCA CTAGCACCAC TGTCCTGGAC 
AGTCAGGCGT TCAAGACTAG CACCATTAGC AATCCAAACA GCGCTCGTCC ATCCTTCAAT 
GTGATCGAAG ATGACGTGGA CCAGGCTCTG AGCAAGGCTG ATGGCACCAT TAAGCGCGAA 
CGTGATAGCA AGCTGTGCCA CCACAATGCC AACGGCCGTT GCGTTCACTG CTCCGCACTG 
GAGCCCTATG ATGAGTCCTA TCTGAAGGAG CACAATATCA AGCACTTGTC CTTCCACTCG 
TACATACGAA AGCAGACATC CGGCATGGAT CAGGGCAAGT ACTTTGTCTT CGACGACATC 
AATTGCCGCA TCAAGCCGGG ATGTCGCGAG CATCCACCAT GGCCCAAGGG CATCTGCTCC 
AAGTGCCAGC CGTCGGCCAT TACGCTGAAT CGTCAGACAT ATCGCCACGT GGACAACGTC 
ATGTTCGAGA ACACTAAGAT CGTGGAGCGC TTCCTCAACT ACTGGCGCAC CACAGGACAC 
CAGCGCATGG GATATCTGTA TGGGACGTAC GAGCAGCACA CTGATGTTCC GTTGGGCATT 
CGGGCCAAGG TGGCTGCCAT TTATGAGCCG CCACAAGAGT CTACCAGGGA TTCGATCAAT 
ATTCAGCCGG ATGAGTTCGC CGACGATGTG GATGCCGTAG CCAGTGCGCT GGGACTGAAG 
AAGATTGGTT GGATTTTCAC TGATCTCATC ACGGACGATG CGAGTATCGG CACTGTCAAA 
CAGATCCGTG GCATCGAGTC GCACTTTATT ACGGCCCAGG AGTGCATCAC AGCTGGCGAG 
CTGCAGAATC GACATCCAAA TCCGTGTAAA TATGCCTCCA ATGGCGTCTT CGGCTCTAAG 
TTTGTGACCA TTTGTGTAAC AGGAGATAAA ACCAAACAAG TGCACATGGA AGGCTATGCG 
GTTTCGGCTC AGTGCATGGC TCTGGTGCGC GATAATTGTC TGATACCCAC TAAGGATGCG 
CCCGAGCTGG GCTACGTGCG TGAATCCACG GACAAGCAGT ATGTTCCCGA CGTTTTCTAT 
AAGGAGAAGG ATCTGTACGG CAACGAGGTG CAGCGGCTGG CTCGCCCCCT GCCCGTGGAG 
TATTTGCTGG TGGACGTGCC CGCATCCACA CCGCTGCAAC CCATTTACAC CTTCACCGAG 
TACGACAAGC GCCAGCCATT CCCCATCGAG AACCGCTACA TCGATGGCCA CCTGCAGGAT 
TTCAATGCAC TCAGCTGCTA TCTGTCCGCC TGGGGCGAGG AGGAGTTCCT GGAGGCCATC 
TCCGATTTCC ACCTGCTGGT CTACCTGTAC AAGATGGACA TGCTGCCGCT GCGACAGCAC 
ATGGGTCCGC TGCTGGAGGC AGTTCGCACC AAGAACCCCA ACCAGGCCGC CCAGTTCAAG 
GTCGTGGATG TGTGGAAGCT GCTCGAGTCG CTTATCCAGG CCAGTTCCGG CGGCAGTGGT 
GGAACCACCA GCTATCCCAG TGGTGGAGCT TCGGCCAGTG CCGGAGCTTC TGGATCGGAG 
GCAATGGACT TGGACGCCAA CACCTGGACA TGCAACCACT GCACCTTCAT CAACCGCGGC 
GAGCTAACGT CCTGCGAGAT CTGCTCGCTA CCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Npl4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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