msi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	msi
Entrez Gene ID	43087
Full Name	musashi
Synonyms	CG5099,DMSIDNA,Dmel\CG5099,MSI,Msi,SIDNA,anon-EST:Liang-2.35,clone 2.35,dMSI,dMsi
General protein information	Preferred Names musashi Names CG5099 gene product from transcript CG5099-RF CG5099-PA CG5099-PB CG5099-PC CG5099-PD CG5099-PE CG5099-PF CG5099-PH msi-PA msi-PB msi-PC msi-PD msi-PE msi-PF msi-PH mushashi
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96E2-96E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_170229.3	NP_733108.2	musashi, isoform B
NM_079838.4	NP_524577.1	musashi, isoform A
NM_001276030.1	NP_001262959.1	musashi, isoform E
NM_001276031.1	NP_001262960.1	musashi, isoform F
NM_001276032.1	NP_001262961.1	musashi, isoform H
NM_001260374.2	NP_001247303.1	musashi, isoform C
NM_001260375.2	NP_001247304.1	musashi, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

show specific evidence for a cell-autonomous requirement for Msi family proteins in regulating stem cell differentiation, leading to the identification of an RNA-binding protein required for spermatogonial stem cell maintenance
Title: The RNA-binding protein Musashi is required intrinsically to maintain stem cell identity.

The following msi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the msi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06102	NM_001260374.2 Latest version!	Drosophila melanogaster musashi (msi), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa06102	NM_001276031.1 Latest version!	Drosophila melanogaster musashi (msi), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa06102	NM_170229.3 Latest version!	Drosophila melanogaster musashi (msi), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa06103	NM_079838.4 Latest version!	Drosophila melanogaster musashi (msi), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa08835	NM_001276030.1 Latest version!	Drosophila melanogaster musashi (msi), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa08836	NM_001260375.2 Latest version!	Drosophila melanogaster musashi (msi), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa08837	NM_001276032.1 Latest version!	Drosophila melanogaster musashi (msi), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

msi ( NM_170229.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733108.2 Drosophila melanogaster musashi (msi), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa06102
Clone ID Related Accession (Same CDS sequence)	NM_170229.3 , NM_001276031.1 , NM_001260374.2
Accession Version	NM_170229.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	musashi, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_170229.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGTTCG AGAATCCGGC GGTTGCGGCC AAGTTGCCCT TTCCGTACAA CGTGCCCCCG 
CCCCTGCAGG CGGCGGCGGC AGCAGCGGCG GCTGTCCCAA ATCTAAGGTT TCAGACACCC 
ATTAAAGCGT TTGCCTGCCT GACAGCAGCC ACAAGGTCAG TATCGGAGAT GAACGCCACT 
TCTCTATATG CCGGCAATCC CATGGAGAAC GCGGCCGCAG CAGCCGCCGC CGCAGCAGCG 
GGCTTGATTG ATCCGCACCA CAATCGCGAT CTCCACCAGG CGCTGGTGGC CTCGATAGCC 
AATAATAGTG TGGCCGCCAT CGGCGGTGGC CTGACCACAG CGGCAGTCTT GAAGAGTGCC 
GCTCAGCAGT CCCAGCAGGC GGTGCAACAG AACCAGAACG CGGTAGTGGT GACACCGGGA 
CTGGAACAGC CCAAACAGGA ACCAGCCCAA CAGGCGGCAC TGGCGCTGCT CAAGGAGAAT 
GTAAACGCCT CCGCCGGAGC GGGACAGAAC AACGGTCAGG CGGCCATGGG TGGCAGCAAC 
AAGAGCGGCT CCTCTGGCAG ATCCACGCCC AGCCTGAGCG GAGGCAGTGG CTCCGATCCG 
GCACCCGGCA AACTCTTTGT CGGCGGTCTC AGCTGGCAGA CGTCGTCTGA CAAGCTCAAG 
GAATACTTCA ACATGTTCGG CACCGTCACC GATGTGCTCA TCATGAAGGA TCCCGTCACC 
CAGCGCAGTC GCGGCTTTGG TTTCATCACA TTCCAAGAAC CCTGCACCGT GGAGAAGGTT 
CTCAAGGTGC CCATTCACAC ACTCGATGGC AAGAAGATCG ATCCCAAGCA TGCCACCCCA 
AAGAATCGAC CTCGTCAGGC CAACAAGACC AAGAAGATCT TCGTGGGCGG CGTCTCACAG 
GATACTTCGG CCGAGGAGGT TAAGGCTTAC TTCAGCCAGT TCGGACCCGT CGAGGAGACG 
GTTATGTTGA TGGACCAGCA GACAAAGCGC CACCGTGGCT TCGGATTTGT CACCTTTGAG 
AATGAGGATG TGGTCGATCG CGTATGTGAG ATTCACTTCC ACACCATCAA GAACAAGAAG 
GTCGAGTGCA AGAAGGCACA GCCCAAGGAA GCAGTCACAC CGGCTGCTCA GCTTCTCCAG 
AAGCGCATTA TGTTGGGCAC CCTCGGCGTC CAGCTGCCCA CAGCTCCTGG CCAGCTGATT 
GGAGCCCGTG GTGCCGGCGT GGCCACCATG AACCCACTGG CCATGCTTCA AAATCCCACA 
CAGCTACTGC AATCCCCGGC AGCAGCCGCT GCCGCCCAGC AGGCCGCCCT CATATCACAG 
AACCCATTTC AAGTACAAAA CGCCGCTGCG GCAGCCTCGA TTGCCAATCA GGCTGGCTTC 
GGCAAGCTGT TGACCACATA TCCGCAGACT GCGCTGCATA GCGTCAGATA TGCACCCTAC 
TCGATCCCCG CCAGCGCCGC CACTGCCAAC GCCGCCTTGA TGCAGGCTCA TCAGGCGCAA 
AGCGTGGCCG CCGCTGCCCA TCATCACCAG CAGCAGCAAC AGCAGCAGCA TCATCACCAG 
CAGCAGACCC ACAATGCCCA TGTGGCCGCC GCCCAGCAGC AGCAGCAGAG CCACCACAAC 
GCCGTCTCGA ATCCCGCTTC GCAGGCGCAC TCGGCAGCAG CGGCTGCCGC CCTGGCGGCC 
AATGCCGCGA ATGGGGCAGG TGCCGCTGGA GCCCACAGCC TGGCCGCTGC CGCTCAGCAA 
GCTGGCTTAA TGGCCGGCAA TCCCCTGAAT GCCGCTGCTG CCGCCGCCGC CGCTGCTGCC 
AATCCGGCGG CTGCCTACTC CAACTATGCG CTGGCCAACG TGGACATGTC CAGCTTCCAG 
GGCGTCGATT GGAGCACCAT GTACGGCATG GGCATGTACG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733108.2
CDS	513..2417
Translation	MLFENPAVAAKLPFPYNVPPPLQAAAAAAAAVPNLRFQTPIKAFACLTAATRSVSEMNATSLYAGNPMENAAAAAAAAAAGLIDPHHNRDLHQALVASIANNSVAAIGGGLTTAAVLKSAAQQSQQAVQQNQNAVVVTPGLEQPKQEPAQQAALALLKENVNASAGAGQNNGQAAMGGSNKSGSSGRSTPSLSGGSGSDPAPGKLFVGGLSWQTSSDKLKEYFNMFGTVTDVLIMKDPVTQRSRGFGFITFQEPCTVEKVLKVPIHTLDGKKIDPKHATPKNRPRQANKTKKIFVGGVSQDTSAEEVKAYFSQFGPVEETVMLMDQQTKRHRGFGFVTFENEDVVDRVCEIHFHTIKNKKVECKKAQPKEAVTPAAQLLQKRIMLGTLGVQLPTAPGQLIGARGAGVATMNPLAMLQNPTQLLQSPAAAAAAQQAALISQNPFQVQNAAAAASIANQAGFGKLLTTYPQTALHSVRYAPYSIPASAATANAALMQAHQAQSVAAAAHHHQQQQQQQHHHQQQTHNAHVAAAQQQQQSHHNAVSNPASQAHSAAAAAALAANAANGAGAAGAHSLAAAAQQAGLMAGNPLNAAAAAAAAAANPAAAYSNYALANVDMSSFQGVDWSTMYGMGMYV

Target ORF information:

RefSeq Version	NM_170229.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster musashi (msi), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170229.3

ORF Insert Sequence:

ATGCTGTTCG AGAATCCGGC GGTTGCGGCC AAGTTGCCCT TTCCGTACAA CGTGCCCCCG 
CCCCTGCAGG CGGCGGCGGC AGCAGCGGCG GCTGTCCCAA ATCTAAGGTT TCAGACACCC 
ATTAAAGCGT TTGCCTGCCT GACAGCAGCC ACAAGGTCAG TATCGGAGAT GAACGCCACT 
TCTCTATATG CCGGCAATCC CATGGAGAAC GCGGCCGCAG CAGCCGCCGC CGCAGCAGCG 
GGCTTGATTG ATCCGCACCA CAATCGCGAT CTCCACCAGG CGCTGGTGGC CTCGATAGCC 
AATAATAGTG TGGCCGCCAT CGGCGGTGGC CTGACCACAG CGGCAGTCTT GAAGAGTGCC 
GCTCAGCAGT CCCAGCAGGC GGTGCAACAG AACCAGAACG CGGTAGTGGT GACACCGGGA 
CTGGAACAGC CCAAACAGGA ACCAGCCCAA CAGGCGGCAC TGGCGCTGCT CAAGGAGAAT 
GTAAACGCCT CCGCCGGAGC GGGACAGAAC AACGGTCAGG CGGCCATGGG TGGCAGCAAC 
AAGAGCGGCT CCTCTGGCAG ATCCACGCCC AGCCTGAGCG GAGGCAGTGG CTCCGATCCG 
GCACCCGGCA AACTCTTTGT CGGCGGTCTC AGCTGGCAGA CGTCGTCTGA CAAGCTCAAG 
GAATACTTCA ACATGTTCGG CACCGTCACC GATGTGCTCA TCATGAAGGA TCCCGTCACC 
CAGCGCAGTC GCGGCTTTGG TTTCATCACA TTCCAAGAAC CCTGCACCGT GGAGAAGGTT 
CTCAAGGTGC CCATTCACAC ACTCGATGGC AAGAAGATCG ATCCCAAGCA TGCCACCCCA 
AAGAATCGAC CTCGTCAGGC CAACAAGACC AAGAAGATCT TCGTGGGCGG CGTCTCACAG 
GATACTTCGG CCGAGGAGGT TAAGGCTTAC TTCAGCCAGT TCGGACCCGT CGAGGAGACG 
GTTATGTTGA TGGACCAGCA GACAAAGCGC CACCGTGGCT TCGGATTTGT CACCTTTGAG 
AATGAGGATG TGGTCGATCG CGTATGTGAG ATTCACTTCC ACACCATCAA GAACAAGAAG 
GTCGAGTGCA AGAAGGCACA GCCCAAGGAA GCAGTCACAC CGGCTGCTCA GCTTCTCCAG 
AAGCGCATTA TGTTGGGCAC CCTCGGCGTC CAGCTGCCCA CAGCTCCTGG CCAGCTGATT 
GGAGCCCGTG GTGCCGGCGT GGCCACCATG AACCCACTGG CCATGCTTCA AAATCCCACA 
CAGCTACTGC AATCCCCGGC AGCAGCCGCT GCCGCCCAGC AGGCCGCCCT CATATCACAG 
AACCCATTTC AAGTACAAAA CGCCGCTGCG GCAGCCTCGA TTGCCAATCA GGCTGGCTTC 
GGCAAGCTGT TGACCACATA TCCGCAGACT GCGCTGCATA GCGTCAGATA TGCACCCTAC 
TCGATCCCCG CCAGCGCCGC CACTGCCAAC GCCGCCTTGA TGCAGGCTCA TCAGGCGCAA 
AGCGTGGCCG CCGCTGCCCA TCATCACCAG CAGCAGCAAC AGCAGCAGCA TCATCACCAG 
CAGCAGACCC ACAATGCCCA TGTGGCCGCC GCCCAGCAGC AGCAGCAGAG CCACCACAAC 
GCCGTCTCGA ATCCCGCTTC GCAGGCGCAC TCGGCAGCAG CGGCTGCCGC CCTGGCGGCC 
AATGCCGCGA ATGGGGCAGG TGCCGCTGGA GCCCACAGCC TGGCCGCTGC CGCTCAGCAA 
GCTGGCTTAA TGGCCGGCAA TCCCCTGAAT GCCGCTGCTG CCGCCGCCGC CGCTGCTGCC 
AATCCGGCGG CTGCCTACTC CAACTATGCG CTGGCCAACG TGGACATGTC CAGCTTCCAG 
GGCGTCGATT GGAGCACCAT GTACGGCATG GGCATGTACG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msi ( NM_001276031.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262960.1 Drosophila melanogaster musashi (msi), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa06102
Clone ID Related Accession (Same CDS sequence)	NM_170229.3 , NM_001276031.1 , NM_001260374.2
Accession Version	NM_001276031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	musashi, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGTTCG AGAATCCGGC GGTTGCGGCC AAGTTGCCCT TTCCGTACAA CGTGCCCCCG 
CCCCTGCAGG CGGCGGCGGC AGCAGCGGCG GCTGTCCCAA ATCTAAGGTT TCAGACACCC 
ATTAAAGCGT TTGCCTGCCT GACAGCAGCC ACAAGGTCAG TATCGGAGAT GAACGCCACT 
TCTCTATATG CCGGCAATCC CATGGAGAAC GCGGCCGCAG CAGCCGCCGC CGCAGCAGCG 
GGCTTGATTG ATCCGCACCA CAATCGCGAT CTCCACCAGG CGCTGGTGGC CTCGATAGCC 
AATAATAGTG TGGCCGCCAT CGGCGGTGGC CTGACCACAG CGGCAGTCTT GAAGAGTGCC 
GCTCAGCAGT CCCAGCAGGC GGTGCAACAG AACCAGAACG CGGTAGTGGT GACACCGGGA 
CTGGAACAGC CCAAACAGGA ACCAGCCCAA CAGGCGGCAC TGGCGCTGCT CAAGGAGAAT 
GTAAACGCCT CCGCCGGAGC GGGACAGAAC AACGGTCAGG CGGCCATGGG TGGCAGCAAC 
AAGAGCGGCT CCTCTGGCAG ATCCACGCCC AGCCTGAGCG GAGGCAGTGG CTCCGATCCG 
GCACCCGGCA AACTCTTTGT CGGCGGTCTC AGCTGGCAGA CGTCGTCTGA CAAGCTCAAG 
GAATACTTCA ACATGTTCGG CACCGTCACC GATGTGCTCA TCATGAAGGA TCCCGTCACC 
CAGCGCAGTC GCGGCTTTGG TTTCATCACA TTCCAAGAAC CCTGCACCGT GGAGAAGGTT 
CTCAAGGTGC CCATTCACAC ACTCGATGGC AAGAAGATCG ATCCCAAGCA TGCCACCCCA 
AAGAATCGAC CTCGTCAGGC CAACAAGACC AAGAAGATCT TCGTGGGCGG CGTCTCACAG 
GATACTTCGG CCGAGGAGGT TAAGGCTTAC TTCAGCCAGT TCGGACCCGT CGAGGAGACG 
GTTATGTTGA TGGACCAGCA GACAAAGCGC CACCGTGGCT TCGGATTTGT CACCTTTGAG 
AATGAGGATG TGGTCGATCG CGTATGTGAG ATTCACTTCC ACACCATCAA GAACAAGAAG 
GTCGAGTGCA AGAAGGCACA GCCCAAGGAA GCAGTCACAC CGGCTGCTCA GCTTCTCCAG 
AAGCGCATTA TGTTGGGCAC CCTCGGCGTC CAGCTGCCCA CAGCTCCTGG CCAGCTGATT 
GGAGCCCGTG GTGCCGGCGT GGCCACCATG AACCCACTGG CCATGCTTCA AAATCCCACA 
CAGCTACTGC AATCCCCGGC AGCAGCCGCT GCCGCCCAGC AGGCCGCCCT CATATCACAG 
AACCCATTTC AAGTACAAAA CGCCGCTGCG GCAGCCTCGA TTGCCAATCA GGCTGGCTTC 
GGCAAGCTGT TGACCACATA TCCGCAGACT GCGCTGCATA GCGTCAGATA TGCACCCTAC 
TCGATCCCCG CCAGCGCCGC CACTGCCAAC GCCGCCTTGA TGCAGGCTCA TCAGGCGCAA 
AGCGTGGCCG CCGCTGCCCA TCATCACCAG CAGCAGCAAC AGCAGCAGCA TCATCACCAG 
CAGCAGACCC ACAATGCCCA TGTGGCCGCC GCCCAGCAGC AGCAGCAGAG CCACCACAAC 
GCCGTCTCGA ATCCCGCTTC GCAGGCGCAC TCGGCAGCAG CGGCTGCCGC CCTGGCGGCC 
AATGCCGCGA ATGGGGCAGG TGCCGCTGGA GCCCACAGCC TGGCCGCTGC CGCTCAGCAA 
GCTGGCTTAA TGGCCGGCAA TCCCCTGAAT GCCGCTGCTG CCGCCGCCGC CGCTGCTGCC 
AATCCGGCGG CTGCCTACTC CAACTATGCG CTGGCCAACG TGGACATGTC CAGCTTCCAG 
GGCGTCGATT GGAGCACCAT GTACGGCATG GGCATGTACG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262960.1
CDS	595..2499
Translation	MLFENPAVAAKLPFPYNVPPPLQAAAAAAAAVPNLRFQTPIKAFACLTAATRSVSEMNATSLYAGNPMENAAAAAAAAAAGLIDPHHNRDLHQALVASIANNSVAAIGGGLTTAAVLKSAAQQSQQAVQQNQNAVVVTPGLEQPKQEPAQQAALALLKENVNASAGAGQNNGQAAMGGSNKSGSSGRSTPSLSGGSGSDPAPGKLFVGGLSWQTSSDKLKEYFNMFGTVTDVLIMKDPVTQRSRGFGFITFQEPCTVEKVLKVPIHTLDGKKIDPKHATPKNRPRQANKTKKIFVGGVSQDTSAEEVKAYFSQFGPVEETVMLMDQQTKRHRGFGFVTFENEDVVDRVCEIHFHTIKNKKVECKKAQPKEAVTPAAQLLQKRIMLGTLGVQLPTAPGQLIGARGAGVATMNPLAMLQNPTQLLQSPAAAAAAQQAALISQNPFQVQNAAAAASIANQAGFGKLLTTYPQTALHSVRYAPYSIPASAATANAALMQAHQAQSVAAAAHHHQQQQQQQHHHQQQTHNAHVAAAQQQQQSHHNAVSNPASQAHSAAAAAALAANAANGAGAAGAHSLAAAAQQAGLMAGNPLNAAAAAAAAAANPAAAYSNYALANVDMSSFQGVDWSTMYGMGMYV

Target ORF information:

RefSeq Version	NM_001276031.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster musashi (msi), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276031.1

ORF Insert Sequence:

ATGCTGTTCG AGAATCCGGC GGTTGCGGCC AAGTTGCCCT TTCCGTACAA CGTGCCCCCG 
CCCCTGCAGG CGGCGGCGGC AGCAGCGGCG GCTGTCCCAA ATCTAAGGTT TCAGACACCC 
ATTAAAGCGT TTGCCTGCCT GACAGCAGCC ACAAGGTCAG TATCGGAGAT GAACGCCACT 
TCTCTATATG CCGGCAATCC CATGGAGAAC GCGGCCGCAG CAGCCGCCGC CGCAGCAGCG 
GGCTTGATTG ATCCGCACCA CAATCGCGAT CTCCACCAGG CGCTGGTGGC CTCGATAGCC 
AATAATAGTG TGGCCGCCAT CGGCGGTGGC CTGACCACAG CGGCAGTCTT GAAGAGTGCC 
GCTCAGCAGT CCCAGCAGGC GGTGCAACAG AACCAGAACG CGGTAGTGGT GACACCGGGA 
CTGGAACAGC CCAAACAGGA ACCAGCCCAA CAGGCGGCAC TGGCGCTGCT CAAGGAGAAT 
GTAAACGCCT CCGCCGGAGC GGGACAGAAC AACGGTCAGG CGGCCATGGG TGGCAGCAAC 
AAGAGCGGCT CCTCTGGCAG ATCCACGCCC AGCCTGAGCG GAGGCAGTGG CTCCGATCCG 
GCACCCGGCA AACTCTTTGT CGGCGGTCTC AGCTGGCAGA CGTCGTCTGA CAAGCTCAAG 
GAATACTTCA ACATGTTCGG CACCGTCACC GATGTGCTCA TCATGAAGGA TCCCGTCACC 
CAGCGCAGTC GCGGCTTTGG TTTCATCACA TTCCAAGAAC CCTGCACCGT GGAGAAGGTT 
CTCAAGGTGC CCATTCACAC ACTCGATGGC AAGAAGATCG ATCCCAAGCA TGCCACCCCA 
AAGAATCGAC CTCGTCAGGC CAACAAGACC AAGAAGATCT TCGTGGGCGG CGTCTCACAG 
GATACTTCGG CCGAGGAGGT TAAGGCTTAC TTCAGCCAGT TCGGACCCGT CGAGGAGACG 
GTTATGTTGA TGGACCAGCA GACAAAGCGC CACCGTGGCT TCGGATTTGT CACCTTTGAG 
AATGAGGATG TGGTCGATCG CGTATGTGAG ATTCACTTCC ACACCATCAA GAACAAGAAG 
GTCGAGTGCA AGAAGGCACA GCCCAAGGAA GCAGTCACAC CGGCTGCTCA GCTTCTCCAG 
AAGCGCATTA TGTTGGGCAC CCTCGGCGTC CAGCTGCCCA CAGCTCCTGG CCAGCTGATT 
GGAGCCCGTG GTGCCGGCGT GGCCACCATG AACCCACTGG CCATGCTTCA AAATCCCACA 
CAGCTACTGC AATCCCCGGC AGCAGCCGCT GCCGCCCAGC AGGCCGCCCT CATATCACAG 
AACCCATTTC AAGTACAAAA CGCCGCTGCG GCAGCCTCGA TTGCCAATCA GGCTGGCTTC 
GGCAAGCTGT TGACCACATA TCCGCAGACT GCGCTGCATA GCGTCAGATA TGCACCCTAC 
TCGATCCCCG CCAGCGCCGC CACTGCCAAC GCCGCCTTGA TGCAGGCTCA TCAGGCGCAA 
AGCGTGGCCG CCGCTGCCCA TCATCACCAG CAGCAGCAAC AGCAGCAGCA TCATCACCAG 
CAGCAGACCC ACAATGCCCA TGTGGCCGCC GCCCAGCAGC AGCAGCAGAG CCACCACAAC 
GCCGTCTCGA ATCCCGCTTC GCAGGCGCAC TCGGCAGCAG CGGCTGCCGC CCTGGCGGCC 
AATGCCGCGA ATGGGGCAGG TGCCGCTGGA GCCCACAGCC TGGCCGCTGC CGCTCAGCAA 
GCTGGCTTAA TGGCCGGCAA TCCCCTGAAT GCCGCTGCTG CCGCCGCCGC CGCTGCTGCC 
AATCCGGCGG CTGCCTACTC CAACTATGCG CTGGCCAACG TGGACATGTC CAGCTTCCAG 
GGCGTCGATT GGAGCACCAT GTACGGCATG GGCATGTACG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msi ( NM_001260374.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247303.1 Drosophila melanogaster musashi (msi), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa06102
Clone ID Related Accession (Same CDS sequence)	NM_170229.3 , NM_001276031.1 , NM_001260374.2
Accession Version	NM_001260374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	musashi, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260374.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGTTCG AGAATCCGGC GGTTGCGGCC AAGTTGCCCT TTCCGTACAA CGTGCCCCCG 
CCCCTGCAGG CGGCGGCGGC AGCAGCGGCG GCTGTCCCAA ATCTAAGGTT TCAGACACCC 
ATTAAAGCGT TTGCCTGCCT GACAGCAGCC ACAAGGTCAG TATCGGAGAT GAACGCCACT 
TCTCTATATG CCGGCAATCC CATGGAGAAC GCGGCCGCAG CAGCCGCCGC CGCAGCAGCG 
GGCTTGATTG ATCCGCACCA CAATCGCGAT CTCCACCAGG CGCTGGTGGC CTCGATAGCC 
AATAATAGTG TGGCCGCCAT CGGCGGTGGC CTGACCACAG CGGCAGTCTT GAAGAGTGCC 
GCTCAGCAGT CCCAGCAGGC GGTGCAACAG AACCAGAACG CGGTAGTGGT GACACCGGGA 
CTGGAACAGC CCAAACAGGA ACCAGCCCAA CAGGCGGCAC TGGCGCTGCT CAAGGAGAAT 
GTAAACGCCT CCGCCGGAGC GGGACAGAAC AACGGTCAGG CGGCCATGGG TGGCAGCAAC 
AAGAGCGGCT CCTCTGGCAG ATCCACGCCC AGCCTGAGCG GAGGCAGTGG CTCCGATCCG 
GCACCCGGCA AACTCTTTGT CGGCGGTCTC AGCTGGCAGA CGTCGTCTGA CAAGCTCAAG 
GAATACTTCA ACATGTTCGG CACCGTCACC GATGTGCTCA TCATGAAGGA TCCCGTCACC 
CAGCGCAGTC GCGGCTTTGG TTTCATCACA TTCCAAGAAC CCTGCACCGT GGAGAAGGTT 
CTCAAGGTGC CCATTCACAC ACTCGATGGC AAGAAGATCG ATCCCAAGCA TGCCACCCCA 
AAGAATCGAC CTCGTCAGGC CAACAAGACC AAGAAGATCT TCGTGGGCGG CGTCTCACAG 
GATACTTCGG CCGAGGAGGT TAAGGCTTAC TTCAGCCAGT TCGGACCCGT CGAGGAGACG 
GTTATGTTGA TGGACCAGCA GACAAAGCGC CACCGTGGCT TCGGATTTGT CACCTTTGAG 
AATGAGGATG TGGTCGATCG CGTATGTGAG ATTCACTTCC ACACCATCAA GAACAAGAAG 
GTCGAGTGCA AGAAGGCACA GCCCAAGGAA GCAGTCACAC CGGCTGCTCA GCTTCTCCAG 
AAGCGCATTA TGTTGGGCAC CCTCGGCGTC CAGCTGCCCA CAGCTCCTGG CCAGCTGATT 
GGAGCCCGTG GTGCCGGCGT GGCCACCATG AACCCACTGG CCATGCTTCA AAATCCCACA 
CAGCTACTGC AATCCCCGGC AGCAGCCGCT GCCGCCCAGC AGGCCGCCCT CATATCACAG 
AACCCATTTC AAGTACAAAA CGCCGCTGCG GCAGCCTCGA TTGCCAATCA GGCTGGCTTC 
GGCAAGCTGT TGACCACATA TCCGCAGACT GCGCTGCATA GCGTCAGATA TGCACCCTAC 
TCGATCCCCG CCAGCGCCGC CACTGCCAAC GCCGCCTTGA TGCAGGCTCA TCAGGCGCAA 
AGCGTGGCCG CCGCTGCCCA TCATCACCAG CAGCAGCAAC AGCAGCAGCA TCATCACCAG 
CAGCAGACCC ACAATGCCCA TGTGGCCGCC GCCCAGCAGC AGCAGCAGAG CCACCACAAC 
GCCGTCTCGA ATCCCGCTTC GCAGGCGCAC TCGGCAGCAG CGGCTGCCGC CCTGGCGGCC 
AATGCCGCGA ATGGGGCAGG TGCCGCTGGA GCCCACAGCC TGGCCGCTGC CGCTCAGCAA 
GCTGGCTTAA TGGCCGGCAA TCCCCTGAAT GCCGCTGCTG CCGCCGCCGC CGCTGCTGCC 
AATCCGGCGG CTGCCTACTC CAACTATGCG CTGGCCAACG TGGACATGTC CAGCTTCCAG 
GGCGTCGATT GGAGCACCAT GTACGGCATG GGCATGTACG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247303.1
CDS	595..2499
Translation	MLFENPAVAAKLPFPYNVPPPLQAAAAAAAAVPNLRFQTPIKAFACLTAATRSVSEMNATSLYAGNPMENAAAAAAAAAAGLIDPHHNRDLHQALVASIANNSVAAIGGGLTTAAVLKSAAQQSQQAVQQNQNAVVVTPGLEQPKQEPAQQAALALLKENVNASAGAGQNNGQAAMGGSNKSGSSGRSTPSLSGGSGSDPAPGKLFVGGLSWQTSSDKLKEYFNMFGTVTDVLIMKDPVTQRSRGFGFITFQEPCTVEKVLKVPIHTLDGKKIDPKHATPKNRPRQANKTKKIFVGGVSQDTSAEEVKAYFSQFGPVEETVMLMDQQTKRHRGFGFVTFENEDVVDRVCEIHFHTIKNKKVECKKAQPKEAVTPAAQLLQKRIMLGTLGVQLPTAPGQLIGARGAGVATMNPLAMLQNPTQLLQSPAAAAAAQQAALISQNPFQVQNAAAAASIANQAGFGKLLTTYPQTALHSVRYAPYSIPASAATANAALMQAHQAQSVAAAAHHHQQQQQQQHHHQQQTHNAHVAAAQQQQQSHHNAVSNPASQAHSAAAAAALAANAANGAGAAGAHSLAAAAQQAGLMAGNPLNAAAAAAAAAANPAAAYSNYALANVDMSSFQGVDWSTMYGMGMYV

Target ORF information:

RefSeq Version	NM_001260374.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster musashi (msi), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260374.2

ORF Insert Sequence:

ATGCTGTTCG AGAATCCGGC GGTTGCGGCC AAGTTGCCCT TTCCGTACAA CGTGCCCCCG 
CCCCTGCAGG CGGCGGCGGC AGCAGCGGCG GCTGTCCCAA ATCTAAGGTT TCAGACACCC 
ATTAAAGCGT TTGCCTGCCT GACAGCAGCC ACAAGGTCAG TATCGGAGAT GAACGCCACT 
TCTCTATATG CCGGCAATCC CATGGAGAAC GCGGCCGCAG CAGCCGCCGC CGCAGCAGCG 
GGCTTGATTG ATCCGCACCA CAATCGCGAT CTCCACCAGG CGCTGGTGGC CTCGATAGCC 
AATAATAGTG TGGCCGCCAT CGGCGGTGGC CTGACCACAG CGGCAGTCTT GAAGAGTGCC 
GCTCAGCAGT CCCAGCAGGC GGTGCAACAG AACCAGAACG CGGTAGTGGT GACACCGGGA 
CTGGAACAGC CCAAACAGGA ACCAGCCCAA CAGGCGGCAC TGGCGCTGCT CAAGGAGAAT 
GTAAACGCCT CCGCCGGAGC GGGACAGAAC AACGGTCAGG CGGCCATGGG TGGCAGCAAC 
AAGAGCGGCT CCTCTGGCAG ATCCACGCCC AGCCTGAGCG GAGGCAGTGG CTCCGATCCG 
GCACCCGGCA AACTCTTTGT CGGCGGTCTC AGCTGGCAGA CGTCGTCTGA CAAGCTCAAG 
GAATACTTCA ACATGTTCGG CACCGTCACC GATGTGCTCA TCATGAAGGA TCCCGTCACC 
CAGCGCAGTC GCGGCTTTGG TTTCATCACA TTCCAAGAAC CCTGCACCGT GGAGAAGGTT 
CTCAAGGTGC CCATTCACAC ACTCGATGGC AAGAAGATCG ATCCCAAGCA TGCCACCCCA 
AAGAATCGAC CTCGTCAGGC CAACAAGACC AAGAAGATCT TCGTGGGCGG CGTCTCACAG 
GATACTTCGG CCGAGGAGGT TAAGGCTTAC TTCAGCCAGT TCGGACCCGT CGAGGAGACG 
GTTATGTTGA TGGACCAGCA GACAAAGCGC CACCGTGGCT TCGGATTTGT CACCTTTGAG 
AATGAGGATG TGGTCGATCG CGTATGTGAG ATTCACTTCC ACACCATCAA GAACAAGAAG 
GTCGAGTGCA AGAAGGCACA GCCCAAGGAA GCAGTCACAC CGGCTGCTCA GCTTCTCCAG 
AAGCGCATTA TGTTGGGCAC CCTCGGCGTC CAGCTGCCCA CAGCTCCTGG CCAGCTGATT 
GGAGCCCGTG GTGCCGGCGT GGCCACCATG AACCCACTGG CCATGCTTCA AAATCCCACA 
CAGCTACTGC AATCCCCGGC AGCAGCCGCT GCCGCCCAGC AGGCCGCCCT CATATCACAG 
AACCCATTTC AAGTACAAAA CGCCGCTGCG GCAGCCTCGA TTGCCAATCA GGCTGGCTTC 
GGCAAGCTGT TGACCACATA TCCGCAGACT GCGCTGCATA GCGTCAGATA TGCACCCTAC 
TCGATCCCCG CCAGCGCCGC CACTGCCAAC GCCGCCTTGA TGCAGGCTCA TCAGGCGCAA 
AGCGTGGCCG CCGCTGCCCA TCATCACCAG CAGCAGCAAC AGCAGCAGCA TCATCACCAG 
CAGCAGACCC ACAATGCCCA TGTGGCCGCC GCCCAGCAGC AGCAGCAGAG CCACCACAAC 
GCCGTCTCGA ATCCCGCTTC GCAGGCGCAC TCGGCAGCAG CGGCTGCCGC CCTGGCGGCC 
AATGCCGCGA ATGGGGCAGG TGCCGCTGGA GCCCACAGCC TGGCCGCTGC CGCTCAGCAA 
GCTGGCTTAA TGGCCGGCAA TCCCCTGAAT GCCGCTGCTG CCGCCGCCGC CGCTGCTGCC 
AATCCGGCGG CTGCCTACTC CAACTATGCG CTGGCCAACG TGGACATGTC CAGCTTCCAG 
GGCGTCGATT GGAGCACCAT GTACGGCATG GGCATGTACG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msi ( NM_079838.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524577.1 Drosophila melanogaster musashi (msi), transcript variant A, mRNA.

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CloneID	OFa06103
Clone ID Related Accession (Same CDS sequence)	NM_079838.4
Accession Version	NM_079838.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	musashi, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079838.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGCGC TTCAGGAAGG CGCCACCGTG CTGCACCACC AGCAGCCACC GCCACCCACT 
TCTGGCGAGG ATCACCTGCT CACCGCTGAT AGTTTCTTTT ATGCACGCAG CAATCCCATG 
GAGAACGCGG CCGCAGCAGC CGCCGCCGCA GCAGCGGGCT TGATTGATCC GCACCACAAT 
CGCGATCTCC ACCAGGCGCT GGTGGCCTCG ATAGCCAATA ATAGTGTGGC CGCCATCGGC 
GGTGGCCTGA CCACAGCGGC AGTCTTGAAG AGTGCCGCTC AGCAGTCCCA GCAGGCGGTG 
CAACAGAACC AGAACGCGGT AGTGGTGACA CCGGGACTGG AACAGCCCAA ACAGGAACCA 
GCCCAACAGG CGGCACTGGC GCTGCTCAAG GAGAATGTAA ACGCCTCCGC CGGAGCGGGA 
CAGAACAACG GTCAGGCGGC CATGGGTGGC AGCAACAAGA GCGGCTCCTC TGGCAGATCC 
ACGCCCAGCC TGAGCGGAGG CAGTGGCTCC GATCCGGCAC CCGGCAAACT CTTTGTCGGC 
GGTCTCAGCT GGCAGACGTC GTCTGACAAG CTCAAGGAAT ACTTCAACAT GTTCGGCACC 
GTCACCGATG TGCTCATCAT GAAGGATCCC GTCACCCAGC GCAGTCGCGG CTTTGGTTTC 
ATCACATTCC AAGAACCCTG CACCGTGGAG AAGGTTCTCA AGGTGCCCAT TCACACACTC 
GATGGCAAGA AGATCGATCC CAAGCATGCC ACCCCAAAGA ATCGACCTCG TCAGGCCAAC 
AAGACCAAGA AGATCTTCGT GGGCGGCGTC TCACAGGATA CTTCGGCCGA GGAGGTTAAG 
GCTTACTTCA GCCAGTTCGG ACCCGTCGAG GAGACGGTTA TGTTGATGGA CCAGCAGACA 
AAGCGCCACC GTGGCTTCGG ATTTGTCACC TTTGAGAATG AGGATGTGGT CGATCGCGTA 
TGTGAGATTC ACTTCCACAC CATCAAGAAC AAGAAGGTCG AGTGCAAGAA GGCACAGCCC 
AAGGAAGCAG TCACACCGGC TGCTCAGCTT CTCCAGAAGC GCATTATGTT GGGCACCCTC 
GGCGTCCAGC TGCCCACAGC TCCTGGCCAG CTGATTGGAG CCCGTGGTGC CGGCGTGGCC 
ACCATGAACC CACTGGCCAT GCTTCAAAAT CCCACACAGC TACTGCAATC CCCGGCAGCA 
GCCGCTGCCG CCCAGCAGGC CGCCCTCATA TCACAGAACC CATTTCAAGT ACAAAACGCC 
GCTGCGGCAG CCTCGATTGC CAATCAGGCT GGCTTCGGCA AGCTGTTGAC CACATATCCG 
CAGACTGCGC TGCATAGCGT CAGATATGCA CCCTACTCGA TCCCCGCCAG CGCCGCCACT 
GCCAACGCCG CCTTGATGCA GGCTCATCAG GCGCAAAGCG TGGCCGCCGC TGCCCATCAT 
CACCAGCAGC AGCAACAGCA GCAGCATCAT CACCAGCAGC AGACCCACAA TGCCCATGTG 
GCCGCCGCCC AGCAGCAGCA GCAGAGCCAC CACAACGCCG TCTCGAATCC CGCTTCGCAG 
GCGCACTCGG CAGCAGCGGC TGCCGCCCTG GCGGCCAATG CCGCGAATGG GGCAGGTGCC 
GCTGGAGCCC ACAGCCTGGC CGCTGCCGCT CAGCAAGCTG GCTTAATGGC CGGCAATCCC 
CTGAATGCCG CTGCTGCCGC CGCCGCCGCT GCTGCCAATC CGGCGGCTGC CTACTCCAAC 
TATGCGCTGG CCAACGTGGA CATGTCCAGC TTCCAGGGCG TCGATTGGAG CACCATGTAC 
GGCATGGGCA TGTACGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524577.1
CDS	490..2310
Translation	MHALQEGATVLHHQQPPPPTSGEDHLLTADSFFYARSNPMENAAAAAAAAAAGLIDPHHNRDLHQALVASIANNSVAAIGGGLTTAAVLKSAAQQSQQAVQQNQNAVVVTPGLEQPKQEPAQQAALALLKENVNASAGAGQNNGQAAMGGSNKSGSSGRSTPSLSGGSGSDPAPGKLFVGGLSWQTSSDKLKEYFNMFGTVTDVLIMKDPVTQRSRGFGFITFQEPCTVEKVLKVPIHTLDGKKIDPKHATPKNRPRQANKTKKIFVGGVSQDTSAEEVKAYFSQFGPVEETVMLMDQQTKRHRGFGFVTFENEDVVDRVCEIHFHTIKNKKVECKKAQPKEAVTPAAQLLQKRIMLGTLGVQLPTAPGQLIGARGAGVATMNPLAMLQNPTQLLQSPAAAAAAQQAALISQNPFQVQNAAAAASIANQAGFGKLLTTYPQTALHSVRYAPYSIPASAATANAALMQAHQAQSVAAAAHHHQQQQQQQHHHQQQTHNAHVAAAQQQQQSHHNAVSNPASQAHSAAAAAALAANAANGAGAAGAHSLAAAAQQAGLMAGNPLNAAAAAAAAAANPAAAYSNYALANVDMSSFQGVDWSTMYGMGMYV

Target ORF information:

RefSeq Version	NM_079838.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster musashi (msi), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079838.4

ORF Insert Sequence:

ATGCACGCGC TTCAGGAAGG CGCCACCGTG CTGCACCACC AGCAGCCACC GCCACCCACT 
TCTGGCGAGG ATCACCTGCT CACCGCTGAT AGTTTCTTTT ATGCACGCAG CAATCCCATG 
GAGAACGCGG CCGCAGCAGC CGCCGCCGCA GCAGCGGGCT TGATTGATCC GCACCACAAT 
CGCGATCTCC ACCAGGCGCT GGTGGCCTCG ATAGCCAATA ATAGTGTGGC CGCCATCGGC 
GGTGGCCTGA CCACAGCGGC AGTCTTGAAG AGTGCCGCTC AGCAGTCCCA GCAGGCGGTG 
CAACAGAACC AGAACGCGGT AGTGGTGACA CCGGGACTGG AACAGCCCAA ACAGGAACCA 
GCCCAACAGG CGGCACTGGC GCTGCTCAAG GAGAATGTAA ACGCCTCCGC CGGAGCGGGA 
CAGAACAACG GTCAGGCGGC CATGGGTGGC AGCAACAAGA GCGGCTCCTC TGGCAGATCC 
ACGCCCAGCC TGAGCGGAGG CAGTGGCTCC GATCCGGCAC CCGGCAAACT CTTTGTCGGC 
GGTCTCAGCT GGCAGACGTC GTCTGACAAG CTCAAGGAAT ACTTCAACAT GTTCGGCACC 
GTCACCGATG TGCTCATCAT GAAGGATCCC GTCACCCAGC GCAGTCGCGG CTTTGGTTTC 
ATCACATTCC AAGAACCCTG CACCGTGGAG AAGGTTCTCA AGGTGCCCAT TCACACACTC 
GATGGCAAGA AGATCGATCC CAAGCATGCC ACCCCAAAGA ATCGACCTCG TCAGGCCAAC 
AAGACCAAGA AGATCTTCGT GGGCGGCGTC TCACAGGATA CTTCGGCCGA GGAGGTTAAG 
GCTTACTTCA GCCAGTTCGG ACCCGTCGAG GAGACGGTTA TGTTGATGGA CCAGCAGACA 
AAGCGCCACC GTGGCTTCGG ATTTGTCACC TTTGAGAATG AGGATGTGGT CGATCGCGTA 
TGTGAGATTC ACTTCCACAC CATCAAGAAC AAGAAGGTCG AGTGCAAGAA GGCACAGCCC 
AAGGAAGCAG TCACACCGGC TGCTCAGCTT CTCCAGAAGC GCATTATGTT GGGCACCCTC 
GGCGTCCAGC TGCCCACAGC TCCTGGCCAG CTGATTGGAG CCCGTGGTGC CGGCGTGGCC 
ACCATGAACC CACTGGCCAT GCTTCAAAAT CCCACACAGC TACTGCAATC CCCGGCAGCA 
GCCGCTGCCG CCCAGCAGGC CGCCCTCATA TCACAGAACC CATTTCAAGT ACAAAACGCC 
GCTGCGGCAG CCTCGATTGC CAATCAGGCT GGCTTCGGCA AGCTGTTGAC CACATATCCG 
CAGACTGCGC TGCATAGCGT CAGATATGCA CCCTACTCGA TCCCCGCCAG CGCCGCCACT 
GCCAACGCCG CCTTGATGCA GGCTCATCAG GCGCAAAGCG TGGCCGCCGC TGCCCATCAT 
CACCAGCAGC AGCAACAGCA GCAGCATCAT CACCAGCAGC AGACCCACAA TGCCCATGTG 
GCCGCCGCCC AGCAGCAGCA GCAGAGCCAC CACAACGCCG TCTCGAATCC CGCTTCGCAG 
GCGCACTCGG CAGCAGCGGC TGCCGCCCTG GCGGCCAATG CCGCGAATGG GGCAGGTGCC 
GCTGGAGCCC ACAGCCTGGC CGCTGCCGCT CAGCAAGCTG GCTTAATGGC CGGCAATCCC 
CTGAATGCCG CTGCTGCCGC CGCCGCCGCT GCTGCCAATC CGGCGGCTGC CTACTCCAAC 
TATGCGCTGG CCAACGTGGA CATGTCCAGC TTCCAGGGCG TCGATTGGAG CACCATGTAC 
GGCATGGGCA TGTACGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msi ( NM_001276030.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262959.1 Drosophila melanogaster musashi (msi), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa08835
Clone ID Related Accession (Same CDS sequence)	NM_001276030.1
Accession Version	NM_001276030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	musashi, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGTTCG AGAATCCGGC GGTTGCGGCC AAGTTGCCCT TTCCGTACAA CGTGCCCCCG 
CCCCTGCAGG CGGCGGCGGC AGCAGCGGCG GCTGTCCCAA ATCTAAGGTC AGTATCGGAG 
ATGAACGCCA CTTCTCTATA TGCCGGCAAT CCCATGGAGA ACGCGGCCGC AGCAGCCGCC 
GCCGCAGCAG CGGGCTTGAT TGATCCGCAC CACAATCGCG ATCTCCACCA GGCGCTGGTG 
GCCTCGATAG CCAATAATAG TGTGGCCGCC ATCGGCGGTG GCCTGACCAC AGCGGCAGTC 
TTGAAGAGTG CCGCTCAGCA GTCCCAGCAG GCGGTGCAAC AGAACCAGAA CGCGGTAGTG 
GTGACACCGG GACTGGAACA GCCCAAACAG GAACCAGCCC AACAGGCGGC ACTGGCGCTG 
CTCAAGGAGA ATGTAAACGC CTCCGCCGGA GCGGGACAGA ACAACGGTCA GGCGGCCATG 
GGTGGCAGCA ACAAGAGCGG CTCCTCTGGC AGATCCACGC CCAGCCTGAG CGGAGGCAGT 
GGCTCCGATC CGGCACCCGG CAAACTCTTT GTCGGCGGTC TCAGCTGGCA GACGTCGTCT 
GACAAGCTCA AGGAATACTT CAACATGTTC GGCACCGTCA CCGATGTGCT CATCATGAAG 
GATCCCGTCA CCCAGCGCAG TCGCGGCTTT GGTTTCATCA CATTCCAAGA ACCCTGCACC 
GTGGAGAAGG TTCTCAAGGT GCCCATTCAC ACACTCGATG GCAAGAAGAT CGATCCCAAG 
CATGCCACCC CAAAGAATCG ACCTCGTCAG GCCAACAAGA CCAAGAAGAT CTTCGTGGGC 
GGCGTCTCAC AGGATACTTC GGCCGAGGAG GTTAAGGCTT ACTTCAGCCA GTTCGGACCC 
GTCGAGGAGA CGGTTATGTT GATGGACCAG CAGACAAAGC GCCACCGTGG CTTCGGATTT 
GTCACCTTTG AGAATGAGGA TGTGGTCGAT CGCGTATGTG AGATTCACTT CCACACCATC 
AAGAACAAGA AGGTCGAGTG CAAGAAGGCA CAGCCCAAGG AAGCAGTCAC ACCGGCTGCT 
CAGCTTCTCC AGAAGCGCAT TATGTTGGGC ACCCTCGGCG TCCAGCTGCC CACAGCTCCT 
GGCCAGCTGA TTGGAGCCCG TGGTGCCGGC GTGGCCACCA TGAACCCACT GGCCATGCTT 
CAAAATCCCA CACAGCTACT GCAATCCCCG GCAGCAGCCG CTGCCGCCCA GCAGGCCGCC 
CTCATATCAC AGAACCCATT TCAAGTACAA AACGCCGCTG CGGCAGCCTC GATTGCCAAT 
CAGGCTGGCT TCGGCAAGCT GTTGACCACA TATCCGCAGA CTGCGCTGCA TAGCGTCAGA 
TATGCACCCT ACTCGATCCC CGCCAGCGCC GCCACTGCCA ACGCCGCCTT GATGCAGGCT 
CATCAGGCGC AAAGCGTGGC CGCCGCTGCC CATCATCACC AGCAGCAGCA ACAGCAGCAG 
CATCATCACC AGCAGCAGAC CCACAATGCC CATGTGGCCG CCGCCCAGCA GCAGCAGCAG 
AGCCACCACA ACGCCGTCTC GAATCCCGCT TCGCAGGCGC ACTCGGCAGC AGCGGCTGCC 
GCCCTGGCGG CCAATGCCGC GAATGGGGCA GGTGCCGCTG GAGCCCACAG CCTGGCCGCT 
GCCGCTCAGC AAGCTGGCTT AATGGCCGGC AATCCCCTGA ATGCCGCTGC TGCCGCCGCC 
GCCGCTGCTG CCAATCCGGC GGCTGCCTAC TCCAACTATG CGCTGGCCAA CGTGGACATG 
TCCAGCTTCC AGGGCGTCGA TTGGAGCACC ATGTACGGCA TGGGCATGTA CGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262959.1
CDS	595..2451
Translation	MLFENPAVAAKLPFPYNVPPPLQAAAAAAAAVPNLRSVSEMNATSLYAGNPMENAAAAAAAAAAGLIDPHHNRDLHQALVASIANNSVAAIGGGLTTAAVLKSAAQQSQQAVQQNQNAVVVTPGLEQPKQEPAQQAALALLKENVNASAGAGQNNGQAAMGGSNKSGSSGRSTPSLSGGSGSDPAPGKLFVGGLSWQTSSDKLKEYFNMFGTVTDVLIMKDPVTQRSRGFGFITFQEPCTVEKVLKVPIHTLDGKKIDPKHATPKNRPRQANKTKKIFVGGVSQDTSAEEVKAYFSQFGPVEETVMLMDQQTKRHRGFGFVTFENEDVVDRVCEIHFHTIKNKKVECKKAQPKEAVTPAAQLLQKRIMLGTLGVQLPTAPGQLIGARGAGVATMNPLAMLQNPTQLLQSPAAAAAAQQAALISQNPFQVQNAAAAASIANQAGFGKLLTTYPQTALHSVRYAPYSIPASAATANAALMQAHQAQSVAAAAHHHQQQQQQQHHHQQQTHNAHVAAAQQQQQSHHNAVSNPASQAHSAAAAAALAANAANGAGAAGAHSLAAAAQQAGLMAGNPLNAAAAAAAAAANPAAAYSNYALANVDMSSFQGVDWSTMYGMGMYV

Target ORF information:

RefSeq Version	NM_001276030.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster musashi (msi), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276030.1

ORF Insert Sequence:

ATGCTGTTCG AGAATCCGGC GGTTGCGGCC AAGTTGCCCT TTCCGTACAA CGTGCCCCCG 
CCCCTGCAGG CGGCGGCGGC AGCAGCGGCG GCTGTCCCAA ATCTAAGGTC AGTATCGGAG 
ATGAACGCCA CTTCTCTATA TGCCGGCAAT CCCATGGAGA ACGCGGCCGC AGCAGCCGCC 
GCCGCAGCAG CGGGCTTGAT TGATCCGCAC CACAATCGCG ATCTCCACCA GGCGCTGGTG 
GCCTCGATAG CCAATAATAG TGTGGCCGCC ATCGGCGGTG GCCTGACCAC AGCGGCAGTC 
TTGAAGAGTG CCGCTCAGCA GTCCCAGCAG GCGGTGCAAC AGAACCAGAA CGCGGTAGTG 
GTGACACCGG GACTGGAACA GCCCAAACAG GAACCAGCCC AACAGGCGGC ACTGGCGCTG 
CTCAAGGAGA ATGTAAACGC CTCCGCCGGA GCGGGACAGA ACAACGGTCA GGCGGCCATG 
GGTGGCAGCA ACAAGAGCGG CTCCTCTGGC AGATCCACGC CCAGCCTGAG CGGAGGCAGT 
GGCTCCGATC CGGCACCCGG CAAACTCTTT GTCGGCGGTC TCAGCTGGCA GACGTCGTCT 
GACAAGCTCA AGGAATACTT CAACATGTTC GGCACCGTCA CCGATGTGCT CATCATGAAG 
GATCCCGTCA CCCAGCGCAG TCGCGGCTTT GGTTTCATCA CATTCCAAGA ACCCTGCACC 
GTGGAGAAGG TTCTCAAGGT GCCCATTCAC ACACTCGATG GCAAGAAGAT CGATCCCAAG 
CATGCCACCC CAAAGAATCG ACCTCGTCAG GCCAACAAGA CCAAGAAGAT CTTCGTGGGC 
GGCGTCTCAC AGGATACTTC GGCCGAGGAG GTTAAGGCTT ACTTCAGCCA GTTCGGACCC 
GTCGAGGAGA CGGTTATGTT GATGGACCAG CAGACAAAGC GCCACCGTGG CTTCGGATTT 
GTCACCTTTG AGAATGAGGA TGTGGTCGAT CGCGTATGTG AGATTCACTT CCACACCATC 
AAGAACAAGA AGGTCGAGTG CAAGAAGGCA CAGCCCAAGG AAGCAGTCAC ACCGGCTGCT 
CAGCTTCTCC AGAAGCGCAT TATGTTGGGC ACCCTCGGCG TCCAGCTGCC CACAGCTCCT 
GGCCAGCTGA TTGGAGCCCG TGGTGCCGGC GTGGCCACCA TGAACCCACT GGCCATGCTT 
CAAAATCCCA CACAGCTACT GCAATCCCCG GCAGCAGCCG CTGCCGCCCA GCAGGCCGCC 
CTCATATCAC AGAACCCATT TCAAGTACAA AACGCCGCTG CGGCAGCCTC GATTGCCAAT 
CAGGCTGGCT TCGGCAAGCT GTTGACCACA TATCCGCAGA CTGCGCTGCA TAGCGTCAGA 
TATGCACCCT ACTCGATCCC CGCCAGCGCC GCCACTGCCA ACGCCGCCTT GATGCAGGCT 
CATCAGGCGC AAAGCGTGGC CGCCGCTGCC CATCATCACC AGCAGCAGCA ACAGCAGCAG 
CATCATCACC AGCAGCAGAC CCACAATGCC CATGTGGCCG CCGCCCAGCA GCAGCAGCAG 
AGCCACCACA ACGCCGTCTC GAATCCCGCT TCGCAGGCGC ACTCGGCAGC AGCGGCTGCC 
GCCCTGGCGG CCAATGCCGC GAATGGGGCA GGTGCCGCTG GAGCCCACAG CCTGGCCGCT 
GCCGCTCAGC AAGCTGGCTT AATGGCCGGC AATCCCCTGA ATGCCGCTGC TGCCGCCGCC 
GCCGCTGCTG CCAATCCGGC GGCTGCCTAC TCCAACTATG CGCTGGCCAA CGTGGACATG 
TCCAGCTTCC AGGGCGTCGA TTGGAGCACC ATGTACGGCA TGGGCATGTA CGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msi ( NM_001260375.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247304.1 Drosophila melanogaster musashi (msi), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa08836
Clone ID Related Accession (Same CDS sequence)	NM_001260375.2
Accession Version	NM_001260375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	musashi, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260375.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGTTCG AGAATCCGGC GGTTGCGGCC AAGTTGCCCT TTCCGTACAA CGTGCCCCCG 
CCCCTGCAGG CGGCGGCGGC AGCAGCGGCG GCTGTCCCAA ATCTAAGCAA TCCCATGGAG 
AACGCGGCCG CAGCAGCCGC CGCCGCAGCA GCGGGCTTGA TTGATCCGCA CCACAATCGC 
GATCTCCACC AGGCGCTGGT GGCCTCGATA GCCAATAATA GTGTGGCCGC CATCGGCGGT 
GGCCTGACCA CAGCGGCAGT CTTGAAGAGT GCCGCTCAGC AGTCCCAGCA GGCGGTGCAA 
CAGAACCAGA ACGCGGTAGT GGTGACACCG GGACTGGAAC AGCCCAAACA GGAACCAGCC 
CAACAGGCGG CACTGGCGCT GCTCAAGGAG AATGTAAACG CCTCCGCCGG AGCGGGACAG 
AACAACGGTC AGGCGGCCAT GGGTGGCAGC AACAAGAGCG GCTCCTCTGG CAGATCCACG 
CCCAGCCTGA GCGGAGGCAG TGGCTCCGAT CCGGCACCCG GCAAACTCTT TGTCGGCGGT 
CTCAGCTGGC AGACGTCGTC TGACAAGCTC AAGGAATACT TCAACATGTT CGGCACCGTC 
ACCGATGTGC TCATCATGAA GGATCCCGTC ACCCAGCGCA GTCGCGGCTT TGGTTTCATC 
ACATTCCAAG AACCCTGCAC CGTGGAGAAG GTTCTCAAGG TGCCCATTCA CACACTCGAT 
GGCAAGAAGA TCGATCCCAA GCATGCCACC CCAAAGAATC GACCTCGTCA GGCCAACAAG 
ACCAAGAAGA TCTTCGTGGG CGGCGTCTCA CAGGATACTT CGGCCGAGGA GGTTAAGGCT 
TACTTCAGCC AGTTCGGACC CGTCGAGGAG ACGGTTATGT TGATGGACCA GCAGACAAAG 
CGCCACCGTG GCTTCGGATT TGTCACCTTT GAGAATGAGG ATGTGGTCGA TCGCGTATGT 
GAGATTCACT TCCACACCAT CAAGAACAAG AAGGTCGAGT GCAAGAAGGC ACAGCCCAAG 
GAAGCAGTCA CACCGGCTGC TCAGCTTCTC CAGAAGCGCA TTATGTTGGG CACCCTCGGC 
GTCCAGCTGC CCACAGCTCC TGGCCAGCTG ATTGGAGCCC GTGGTGCCGG CGTGGCCACC 
ATGAACCCAC TGGCCATGCT TCAAAATCCC ACACAGCTAC TGCAATCCCC GGCAGCAGCC 
GCTGCCGCCC AGCAGGCCGC CCTCATATCA CAGAACCCAT TTCAAGTACA AAACGCCGCT 
GCGGCAGCCT CGATTGCCAA TCAGGCTGGC TTCGGCAAGC TGTTGACCAC ATATCCGCAG 
ACTGCGCTGC ATAGCGTCAG ATATGCACCC TACTCGATCC CCGCCAGCGC CGCCACTGCC 
AACGCCGCCT TGATGCAGGC TCATCAGGCG CAAAGCGTGG CCGCCGCTGC CCATCATCAC 
CAGCAGCAGC AACAGCAGCA GCATCATCAC CAGCAGCAGA CCCACAATGC CCATGTGGCC 
GCCGCCCAGC AGCAGCAGCA GAGCCACCAC AACGCCGTCT CGAATCCCGC TTCGCAGGCG 
CACTCGGCAG CAGCGGCTGC CGCCCTGGCG GCCAATGCCG CGAATGGGGC AGGTGCCGCT 
GGAGCCCACA GCCTGGCCGC TGCCGCTCAG CAAGCTGGCT TAATGGCCGG CAATCCCCTG 
AATGCCGCTG CTGCCGCCGC CGCCGCTGCT GCCAATCCGG CGGCTGCCTA CTCCAACTAT 
GCGCTGGCCA ACGTGGACAT GTCCAGCTTC CAGGGCGTCG ATTGGAGCAC CATGTACGGC 
ATGGGCATGT ACGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247304.1
CDS	595..2412
Translation	MLFENPAVAAKLPFPYNVPPPLQAAAAAAAAVPNLSNPMENAAAAAAAAAAGLIDPHHNRDLHQALVASIANNSVAAIGGGLTTAAVLKSAAQQSQQAVQQNQNAVVVTPGLEQPKQEPAQQAALALLKENVNASAGAGQNNGQAAMGGSNKSGSSGRSTPSLSGGSGSDPAPGKLFVGGLSWQTSSDKLKEYFNMFGTVTDVLIMKDPVTQRSRGFGFITFQEPCTVEKVLKVPIHTLDGKKIDPKHATPKNRPRQANKTKKIFVGGVSQDTSAEEVKAYFSQFGPVEETVMLMDQQTKRHRGFGFVTFENEDVVDRVCEIHFHTIKNKKVECKKAQPKEAVTPAAQLLQKRIMLGTLGVQLPTAPGQLIGARGAGVATMNPLAMLQNPTQLLQSPAAAAAAQQAALISQNPFQVQNAAAAASIANQAGFGKLLTTYPQTALHSVRYAPYSIPASAATANAALMQAHQAQSVAAAAHHHQQQQQQQHHHQQQTHNAHVAAAQQQQQSHHNAVSNPASQAHSAAAAAALAANAANGAGAAGAHSLAAAAQQAGLMAGNPLNAAAAAAAAAANPAAAYSNYALANVDMSSFQGVDWSTMYGMGMYV

Target ORF information:

RefSeq Version	NM_001260375.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster musashi (msi), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260375.2

ORF Insert Sequence:

ATGCTGTTCG AGAATCCGGC GGTTGCGGCC AAGTTGCCCT TTCCGTACAA CGTGCCCCCG 
CCCCTGCAGG CGGCGGCGGC AGCAGCGGCG GCTGTCCCAA ATCTAAGCAA TCCCATGGAG 
AACGCGGCCG CAGCAGCCGC CGCCGCAGCA GCGGGCTTGA TTGATCCGCA CCACAATCGC 
GATCTCCACC AGGCGCTGGT GGCCTCGATA GCCAATAATA GTGTGGCCGC CATCGGCGGT 
GGCCTGACCA CAGCGGCAGT CTTGAAGAGT GCCGCTCAGC AGTCCCAGCA GGCGGTGCAA 
CAGAACCAGA ACGCGGTAGT GGTGACACCG GGACTGGAAC AGCCCAAACA GGAACCAGCC 
CAACAGGCGG CACTGGCGCT GCTCAAGGAG AATGTAAACG CCTCCGCCGG AGCGGGACAG 
AACAACGGTC AGGCGGCCAT GGGTGGCAGC AACAAGAGCG GCTCCTCTGG CAGATCCACG 
CCCAGCCTGA GCGGAGGCAG TGGCTCCGAT CCGGCACCCG GCAAACTCTT TGTCGGCGGT 
CTCAGCTGGC AGACGTCGTC TGACAAGCTC AAGGAATACT TCAACATGTT CGGCACCGTC 
ACCGATGTGC TCATCATGAA GGATCCCGTC ACCCAGCGCA GTCGCGGCTT TGGTTTCATC 
ACATTCCAAG AACCCTGCAC CGTGGAGAAG GTTCTCAAGG TGCCCATTCA CACACTCGAT 
GGCAAGAAGA TCGATCCCAA GCATGCCACC CCAAAGAATC GACCTCGTCA GGCCAACAAG 
ACCAAGAAGA TCTTCGTGGG CGGCGTCTCA CAGGATACTT CGGCCGAGGA GGTTAAGGCT 
TACTTCAGCC AGTTCGGACC CGTCGAGGAG ACGGTTATGT TGATGGACCA GCAGACAAAG 
CGCCACCGTG GCTTCGGATT TGTCACCTTT GAGAATGAGG ATGTGGTCGA TCGCGTATGT 
GAGATTCACT TCCACACCAT CAAGAACAAG AAGGTCGAGT GCAAGAAGGC ACAGCCCAAG 
GAAGCAGTCA CACCGGCTGC TCAGCTTCTC CAGAAGCGCA TTATGTTGGG CACCCTCGGC 
GTCCAGCTGC CCACAGCTCC TGGCCAGCTG ATTGGAGCCC GTGGTGCCGG CGTGGCCACC 
ATGAACCCAC TGGCCATGCT TCAAAATCCC ACACAGCTAC TGCAATCCCC GGCAGCAGCC 
GCTGCCGCCC AGCAGGCCGC CCTCATATCA CAGAACCCAT TTCAAGTACA AAACGCCGCT 
GCGGCAGCCT CGATTGCCAA TCAGGCTGGC TTCGGCAAGC TGTTGACCAC ATATCCGCAG 
ACTGCGCTGC ATAGCGTCAG ATATGCACCC TACTCGATCC CCGCCAGCGC CGCCACTGCC 
AACGCCGCCT TGATGCAGGC TCATCAGGCG CAAAGCGTGG CCGCCGCTGC CCATCATCAC 
CAGCAGCAGC AACAGCAGCA GCATCATCAC CAGCAGCAGA CCCACAATGC CCATGTGGCC 
GCCGCCCAGC AGCAGCAGCA GAGCCACCAC AACGCCGTCT CGAATCCCGC TTCGCAGGCG 
CACTCGGCAG CAGCGGCTGC CGCCCTGGCG GCCAATGCCG CGAATGGGGC AGGTGCCGCT 
GGAGCCCACA GCCTGGCCGC TGCCGCTCAG CAAGCTGGCT TAATGGCCGG CAATCCCCTG 
AATGCCGCTG CTGCCGCCGC CGCCGCTGCT GCCAATCCGG CGGCTGCCTA CTCCAACTAT 
GCGCTGGCCA ACGTGGACAT GTCCAGCTTC CAGGGCGTCG ATTGGAGCAC CATGTACGGC 
ATGGGCATGT ACGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msi ( NM_001276032.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262961.1 Drosophila melanogaster musashi (msi), transcript variant H, mRNA.

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CloneID	OFa08837
Clone ID Related Accession (Same CDS sequence)	NM_001276032.1
Accession Version	NM_001276032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	musashi, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACG CGGCCGCAGC AGCCGCCGCC GCAGCAGCGG GCTTGATTGA TCCGCACCAC 
AATCGCGATC TCCACCAGGC GCTGGTGGCC TCGATAGCCA ATAATAGTGT GGCCGCCATC 
GGCGGTGGCC TGACCACAGC GGCAGTCTTG AAGAGTGCCG CTCAGCAGTC CCAGCAGGCG 
GTGCAACAGA ACCAGAACGC GGTAGTGGTG ACACCGGGAC TGGAACAGCC CAAACAGGAA 
CCAGCCCAAC AGGCGGCACT GGCGCTGCTC AAGGAGAATG TAAACGCCTC CGCCGGAGCG 
GGACAGAACA ACGGTCAGGC GGCCATGGGT GGCAGCAACA AGAGCGGCTC CTCTGGCAGA 
TCCACGCCCA GCCTGAGCGG AGGCAGTGGC TCCGATCCGG CACCCGGCAA ACTCTTTGTC 
GGCGGTCTCA GCTGGCAGAC GTCGTCTGAC AAGCTCAAGG AATACTTCAA CATGTTCGGC 
ACCGTCACCG ATGTGCTCAT CATGAAGGAT CCCGTCACCC AGCGCAGTCG CGGCTTTGGT 
TTCATCACAT TCCAAGAACC CTGCACCGTG GAGAAGGTTC TCAAGGTGCC CATTCACACA 
CTCGATGGCA AGAAGATCGA TCCCAAGCAT GCCACCCCAA AGAATCGACC TCGTCAGGCC 
AACAAGACCA AGAAGATCTT CGTGGGCGGC GTCTCACAGG ATACTTCGGC CGAGGAGGTT 
AAGGCTTACT TCAGCCAGTT CGGACCCGTC GAGGAGACGG TTATGTTGAT GGACCAGCAG 
ACAAAGCGCC ACCGTGGCTT CGGATTTGTC ACCTTTGAGA ATGAGGATGT GGTCGATCGC 
GTATGTGAGA TTCACTTCCA CACCATCAAG AACAAGAAGG TCGAGTGCAA GAAGGCACAG 
CCCAAGGAAG CAGTCACACC GGCTGCTCAG CTTCTCCAGA AGCGCATTAT GTTGGGCACC 
CTCGGCGTCC AGCTGCCCAC AGCTCCTGGC CAGCTGATTG GAGCCCGTGG TGCCGGCGTG 
GCCACCATGA ACCCACTGGC CATGCTTCAA AATCCCACAC AGCTACTGCA ATCCCCGGCA 
GCAGCCGCTG CCGCCCAGCA GGCCGCCCTC ATATCACAGA ACCCATTTCA AGTACAAAAC 
GCCGCTGCGG CAGCCTCGAT TGCCAATCAG GCTGGCTTCG GCAAGCTGTT GACCACATAT 
CCGCAGACTG CGCTGCATAG CGTCAGATAT GCACCCTACT CGATCCCCGC CAGCGCCGCC 
ACTGCCAACG CCGCCTTGAT GCAGGCTCAT CAGGCGCAAA GCGTGGCCGC CGCTGCCCAT 
CATCACCAGC AGCAGCAACA GCAGCAGCAT CATCACCAGC AGCAGACCCA CAATGCCCAT 
GTGGCCGCCG CCCAGCAGCA GCAGCAGAGC CACCACAACG CCGTCTCGAA TCCCGCTTCG 
CAGGCGCACT CGGCAGCAGC GGCTGCCGCC CTGGCGGCCA ATGCCGCGAA TGGGGCAGGT 
GCCGCTGGAG CCCACAGCCT GGCCGCTGCC GCTCAGCAAG CTGGCTTAAT GGCCGGCAAT 
CCCCTGAATG CCGCTGCTGC CGCCGCCGCC GCTGCTGCCA ATCCGGCGGC TGCCTACTCC 
AACTATGCGC TGGCCAACGT GGACATGTCC AGCTTCCAGG GCGTCGATTG GAGCACCATG 
TACGGCATGG GCATGTACGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262961.1
CDS	584..2287
Translation	MENAAAAAAAAAAGLIDPHHNRDLHQALVASIANNSVAAIGGGLTTAAVLKSAAQQSQQAVQQNQNAVVVTPGLEQPKQEPAQQAALALLKENVNASAGAGQNNGQAAMGGSNKSGSSGRSTPSLSGGSGSDPAPGKLFVGGLSWQTSSDKLKEYFNMFGTVTDVLIMKDPVTQRSRGFGFITFQEPCTVEKVLKVPIHTLDGKKIDPKHATPKNRPRQANKTKKIFVGGVSQDTSAEEVKAYFSQFGPVEETVMLMDQQTKRHRGFGFVTFENEDVVDRVCEIHFHTIKNKKVECKKAQPKEAVTPAAQLLQKRIMLGTLGVQLPTAPGQLIGARGAGVATMNPLAMLQNPTQLLQSPAAAAAAQQAALISQNPFQVQNAAAAASIANQAGFGKLLTTYPQTALHSVRYAPYSIPASAATANAALMQAHQAQSVAAAAHHHQQQQQQQHHHQQQTHNAHVAAAQQQQQSHHNAVSNPASQAHSAAAAAALAANAANGAGAAGAHSLAAAAQQAGLMAGNPLNAAAAAAAAAANPAAAYSNYALANVDMSSFQGVDWSTMYGMGMYV

Target ORF information:

RefSeq Version	NM_001276032.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster musashi (msi), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276032.1

ORF Insert Sequence:

ATGGAGAACG CGGCCGCAGC AGCCGCCGCC GCAGCAGCGG GCTTGATTGA TCCGCACCAC 
AATCGCGATC TCCACCAGGC GCTGGTGGCC TCGATAGCCA ATAATAGTGT GGCCGCCATC 
GGCGGTGGCC TGACCACAGC GGCAGTCTTG AAGAGTGCCG CTCAGCAGTC CCAGCAGGCG 
GTGCAACAGA ACCAGAACGC GGTAGTGGTG ACACCGGGAC TGGAACAGCC CAAACAGGAA 
CCAGCCCAAC AGGCGGCACT GGCGCTGCTC AAGGAGAATG TAAACGCCTC CGCCGGAGCG 
GGACAGAACA ACGGTCAGGC GGCCATGGGT GGCAGCAACA AGAGCGGCTC CTCTGGCAGA 
TCCACGCCCA GCCTGAGCGG AGGCAGTGGC TCCGATCCGG CACCCGGCAA ACTCTTTGTC 
GGCGGTCTCA GCTGGCAGAC GTCGTCTGAC AAGCTCAAGG AATACTTCAA CATGTTCGGC 
ACCGTCACCG ATGTGCTCAT CATGAAGGAT CCCGTCACCC AGCGCAGTCG CGGCTTTGGT 
TTCATCACAT TCCAAGAACC CTGCACCGTG GAGAAGGTTC TCAAGGTGCC CATTCACACA 
CTCGATGGCA AGAAGATCGA TCCCAAGCAT GCCACCCCAA AGAATCGACC TCGTCAGGCC 
AACAAGACCA AGAAGATCTT CGTGGGCGGC GTCTCACAGG ATACTTCGGC CGAGGAGGTT 
AAGGCTTACT TCAGCCAGTT CGGACCCGTC GAGGAGACGG TTATGTTGAT GGACCAGCAG 
ACAAAGCGCC ACCGTGGCTT CGGATTTGTC ACCTTTGAGA ATGAGGATGT GGTCGATCGC 
GTATGTGAGA TTCACTTCCA CACCATCAAG AACAAGAAGG TCGAGTGCAA GAAGGCACAG 
CCCAAGGAAG CAGTCACACC GGCTGCTCAG CTTCTCCAGA AGCGCATTAT GTTGGGCACC 
CTCGGCGTCC AGCTGCCCAC AGCTCCTGGC CAGCTGATTG GAGCCCGTGG TGCCGGCGTG 
GCCACCATGA ACCCACTGGC CATGCTTCAA AATCCCACAC AGCTACTGCA ATCCCCGGCA 
GCAGCCGCTG CCGCCCAGCA GGCCGCCCTC ATATCACAGA ACCCATTTCA AGTACAAAAC 
GCCGCTGCGG CAGCCTCGAT TGCCAATCAG GCTGGCTTCG GCAAGCTGTT GACCACATAT 
CCGCAGACTG CGCTGCATAG CGTCAGATAT GCACCCTACT CGATCCCCGC CAGCGCCGCC 
ACTGCCAACG CCGCCTTGAT GCAGGCTCAT CAGGCGCAAA GCGTGGCCGC CGCTGCCCAT 
CATCACCAGC AGCAGCAACA GCAGCAGCAT CATCACCAGC AGCAGACCCA CAATGCCCAT 
GTGGCCGCCG CCCAGCAGCA GCAGCAGAGC CACCACAACG CCGTCTCGAA TCCCGCTTCG 
CAGGCGCACT CGGCAGCAGC GGCTGCCGCC CTGGCGGCCA ATGCCGCGAA TGGGGCAGGT 
GCCGCTGGAG CCCACAGCCT GGCCGCTGCC GCTCAGCAAG CTGGCTTAAT GGCCGGCAAT 
CCCCTGAATG CCGCTGCTGC CGCCGCCGCC GCTGCTGCCA ATCCGGCGGC TGCCTACTCC 
AACTATGCGC TGGCCAACGT GGACATGTCC AGCTTCCAGG GCGTCGATTG GAGCACCATG 
TACGGCATGG GCATGTACGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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msi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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