Hr96 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hr96
Entrez Gene ID	42993
Full Name	Hormone receptor-like in 96
Synonyms	CG11783,DHR96,Dhr96,Dmel\CG11783,HR96,NR1J1,dHR96
General protein information	Preferred Names Hormone receptor-like in 96 Names CG11783 gene product from transcript CG11783-RA CG11783-PA Hr96-PA hormone receptor-like in 96
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96B16-96B17

mRNA and Protein(s)

mRNA	Protein	Name
NM_079769.3	NP_524493.1	hormone receptor-like in 96

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NR1I2	NP_001096696.1
Danio rerio (zebrafish)	nr1i2	NP_001092087.1
Xenopus tropicalis (tropical clawed frog)	nr1i2	NP_001091887.1
Pan troglodytes (chimpanzee)	NR1I2	XP_001164074.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004224	XP_313130.5
Drosophila melanogaster (fruit fly)	Hr96	NP_524493.1
Homo sapiens (human)	NR1I2	NP_071285.1
	NR1I2	NP_001028054.3
Mus musculus (house mouse)	Nr1i2	NP_035066.1
Rattus norvegicus (Norway rat)	Nr1i2	NP_443212.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identified a nuclear hormone receptor, DHR96, as an essential mediator of the increased xenobiotic resistance of IIS mutant flies. However, the IIS mutants remained long-lived in the absence of DHR96 and the xenobiotic resistance that it conferred.
Title: Nuclear hormone receptor DHR96 mediates the resistance to xenobiotics but not the increased lifespan of insulin-mutant Drosophila.

DHR96 coordinates lipid metabolism through direct regulation of LipA homolog magro proteins.
Title: Coordination of triacylglycerol and cholesterol homeostasis by DHR96 and the Drosophila LipA homolog magro.

DHR96 is activated when cellular cholesterol concentrations drop below a critical threshold in order to protect cells from severe cholesterol deprivation.
Title: Nuclear receptor DHR96 acts as a sentinel for low cholesterol concentrations in Drosophila melanogaster.

DHR96 function is required in the midgut for the breakdown of dietary fat
Title: The DHR96 nuclear receptor controls triacylglycerol homeostasis in Drosophila.

The following Hr96 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hr96 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08786	NM_079769.3 Latest version!	Drosophila melanogaster hormone receptor-like in 96 (Hr96), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hr96 ( NM_079769.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524493.1 Drosophila melanogaster hormone receptor-like in 96 (Hr96), mRNA.

Price & Availability↑

CloneID	OFa08786
Clone ID Related Accession (Same CDS sequence)	NM_079769.3
Accession Version	NM_079769.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hormone receptor-like in 96
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079769.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCCGC CGAAGAACTG CGCGGTGTGC GGGGACAAGG CTCTGGGCTA CAACTTCAAT 
GCGGTCACCT GCGAGAGCTG CAAGGCGTTC TTCCGACGGA ACGCGCTGGC CAAGAAGCAG 
TTCACCTGCC CCTTCAACCA AAACTGCGAC ATCACTGTGG TCACTCGACG CTTCTGCCAG 
AAATGCCGCC TGCGCAAGTG CCTGGATATC GGGATGAAGA GTGAAAACAT TATGTCCGAG 
GAGGACAAGC TGATCAAGCG GCGCAAGATC GAGACCAACC GGGCCAAGCG ACGCCTCATG 
GAGAACGGCA CGGATGCGTG CGACGCCGAT GGCGGCGAGG AAAGGGATCA CAAAGCGCCG 
GCGGATAGCA GCAGCAGCAA CCTTGACCAC TACTCGGGGT CACAGGACTC GCAGAGCTGC 
GGCTCGGCGG ACAGCGGGGC CAATGGGTGC TCCGGCAGAC AGGCCAGTTC GCCGGGCACA 
CAGGTCAATC CGCTTCAGAT GACGGCCGAG AAGATAGTCG ACCAGATCGT ATCCGACCCG 
GATCGAGCCT CGCAGGCCAT CAACCGGTTG ATGCGCACGC AGAAAGAGGC TATATCGGTG 
ATGGAGAAGG TAATCAGCTC ACAAAAGGAC GCCTTAAGGC TGGTGTCGCA TTTGATCGAC 
TATCCAGGCG ACGCACTCAA GATCATTTCA AAGTTTATGA ACTCGCCCTT TAACGCGCTG 
ACAGTATTCA CCAAATTCAT GAGCTCACCC ACGGACGGCG TTGAAATTAT CTCAAAGATA 
GTTGATTCGC CCGCGGACGT GGTGGAGTTC ATGCAGAACT TGATGCACTC GCCAGAGGAC 
GCCATCGATA TAATGAACAA GTTCATGAAT ACCCCAGCGG AGGCGCTGCG CATTCTTAAC 
CGAATCCTAA GCGGCGGAGG AGCGAACGCA GCCCAGCAGA CAGCAGACCG CAAGCCATTG 
CTGGACAAGG AGCCGGCGGT GAAGCCTGCA GCGCCAGCGG AGCGAGCTGA TACTGTCATT 
CAAAGCATGC TGGGCAACAG TCCGCCAATT TCGCCACATG ATGCTGCCGT GGATCTGCAG 
TACCACTCGC CCGGTGTCGG GGAGCAGCCC AGTACATCGA GTAGCCACCC CTTGCCTTAC 
ATAGCCAACT CGCCGGACTT CGATCTGAAG ACCTTCATGC AGACCAACTA CAACGACGAG 
CCCAGTCTGG ACAGTGATTT TAGCATTAAC TCAATCGAAT CGGTGCTATC CGAGGTGATC 
CGCATTGAGT ACCAGGCCTT CAATAGCATA CAACAAGCGG CATCGCGCGT AAAGGAGGAG 
ATGTCCTACG GCACTCAGTC TACGTACGGT GGATGCAATT CGGCTGCAAA CAATAGCCAG 
CCGCACCTGC AGCAACCCAT CTGCGCCCCA TCCACCCAGC AGTTGGATCG CGAGCTAAAC 
GAGGCGGAGC AAATGAAGCT GCGGGAGCTG CGACTGGCCA GCGAGGCTCT TTATGATCCC 
GTGGACGAGG ACCTCAGCGC CCTGATGATG GGCGATGATC GCATTAAGCC CGACGACACT 
CGCCACAACC CAAAGCTATT GCAGCTGATC AATCTGACGG CGGTGGCCAT CAAGCGGCTT 
ATCAAAATGG CCAAGAAGAT TACAGCATTC CGTGACATGT GCCAGGAGGA CCAGGTGGCC 
CTACTCAAAG GTGGCTGCAC AGAAATGATG ATAATGCGCT CCGTAATGAT TTACGACGAC 
GATCGCGCCG CCTGGAAGGT ACCCCATACC AAAGAGAACA TGGGCAACAT ACGCACTGAC 
CTGCTCAAGT TTGCCGAAGG CAATATCTAC GAGGAGCACC AAAAGTTCAT CACAACGTTT 
GACGAGAAGT GGCGCATGGA CGAGAACATA ATCCTGATCA TGTGTGCCAT TGTCCTTTTT 
ACCTCGGCTC GATCGCGAGT GATACACAAA GACGTGATTA GATTGGAACA GAATTCCTAC 
TATTATCTTC TGCGAAGATA TCTGGAGAGT GTTTATTCTG GCTGTGAGGC GAGAAACGCG 
TTTATCAAGC TAATCCAAAA GATTTCAGAT GTGGAGCGTC TGAACAAGTT CATAATTAAT 
GTCTATTTGA ATGTTAACCC ATCCCAGGTG GAGCCCTTGC TGCGTGAAAT ATTCGATTTG 
AAAAATCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524493.1
CDS	310..2481
Translation	MSPPKNCAVCGDKALGYNFNAVTCESCKAFFRRNALAKKQFTCPFNQNCDITVVTRRFCQKCRLRKCLDIGMKSENIMSEEDKLIKRRKIETNRAKRRLMENGTDACDADGGEERDHKAPADSSSSNLDHYSGSQDSQSCGSADSGANGCSGRQASSPGTQVNPLQMTAEKIVDQIVSDPDRASQAINRLMRTQKEAISVMEKVISSQKDALRLVSHLIDYPGDALKIISKFMNSPFNALTVFTKFMSSPTDGVEIISKIVDSPADVVEFMQNLMHSPEDAIDIMNKFMNTPAEALRILNRILSGGGANAAQQTADRKPLLDKEPAVKPAAPAERADTVIQSMLGNSPPISPHDAAVDLQYHSPGVGEQPSTSSSHPLPYIANSPDFDLKTFMQTNYNDEPSLDSDFSINSIESVLSEVIRIEYQAFNSIQQAASRVKEEMSYGTQSTYGGCNSAANNSQPHLQQPICAPSTQQLDRELNEAEQMKLRELRLASEALYDPVDEDLSALMMGDDRIKPDDTRHNPKLLQLINLTAVAIKRLIKMAKKITAFRDMCQEDQVALLKGGCTEMMIMRSVMIYDDDRAAWKVPHTKENMGNIRTDLLKFAEGNIYEEHQKFITTFDEKWRMDENIILIMCAIVLFTSARSRVIHKDVIRLEQNSYYYLLRRYLESVYSGCEARNAFIKLIQKISDVERLNKFIINVYLNVNPSQVEPLLREIFDLKNH

Target ORF information:

RefSeq Version	NM_079769.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hormone receptor-like in 96 (Hr96), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079769.3

ORF Insert Sequence:

ATGTCGCCGC CGAAGAACTG CGCGGTGTGC GGGGACAAGG CTCTGGGCTA CAACTTCAAT 
GCGGTCACCT GCGAGAGCTG CAAGGCGTTC TTCCGACGGA ACGCGCTGGC CAAGAAGCAG 
TTCACCTGCC CCTTCAACCA AAACTGCGAC ATCACTGTGG TCACTCGACG CTTCTGCCAG 
AAATGCCGCC TGCGCAAGTG CCTGGATATC GGGATGAAGA GTGAAAACAT TATGTCCGAG 
GAGGACAAGC TGATCAAGCG GCGCAAGATC GAGACCAACC GGGCCAAGCG ACGCCTCATG 
GAGAACGGCA CGGATGCGTG CGACGCCGAT GGCGGCGAGG AAAGGGATCA CAAAGCGCCG 
GCGGATAGCA GCAGCAGCAA CCTTGACCAC TACTCGGGGT CACAGGACTC GCAGAGCTGC 
GGCTCGGCGG ACAGCGGGGC CAATGGGTGC TCCGGCAGAC AGGCCAGTTC GCCGGGCACA 
CAGGTCAATC CGCTTCAGAT GACGGCCGAG AAGATAGTCG ACCAGATCGT ATCCGACCCG 
GATCGAGCCT CGCAGGCCAT CAACCGGTTG ATGCGCACGC AGAAAGAGGC TATATCGGTG 
ATGGAGAAGG TAATCAGCTC ACAAAAGGAC GCCTTAAGGC TGGTGTCGCA TTTGATCGAC 
TATCCAGGCG ACGCACTCAA GATCATTTCA AAGTTTATGA ACTCGCCCTT TAACGCGCTG 
ACAGTATTCA CCAAATTCAT GAGCTCACCC ACGGACGGCG TTGAAATTAT CTCAAAGATA 
GTTGATTCGC CCGCGGACGT GGTGGAGTTC ATGCAGAACT TGATGCACTC GCCAGAGGAC 
GCCATCGATA TAATGAACAA GTTCATGAAT ACCCCAGCGG AGGCGCTGCG CATTCTTAAC 
CGAATCCTAA GCGGCGGAGG AGCGAACGCA GCCCAGCAGA CAGCAGACCG CAAGCCATTG 
CTGGACAAGG AGCCGGCGGT GAAGCCTGCA GCGCCAGCGG AGCGAGCTGA TACTGTCATT 
CAAAGCATGC TGGGCAACAG TCCGCCAATT TCGCCACATG ATGCTGCCGT GGATCTGCAG 
TACCACTCGC CCGGTGTCGG GGAGCAGCCC AGTACATCGA GTAGCCACCC CTTGCCTTAC 
ATAGCCAACT CGCCGGACTT CGATCTGAAG ACCTTCATGC AGACCAACTA CAACGACGAG 
CCCAGTCTGG ACAGTGATTT TAGCATTAAC TCAATCGAAT CGGTGCTATC CGAGGTGATC 
CGCATTGAGT ACCAGGCCTT CAATAGCATA CAACAAGCGG CATCGCGCGT AAAGGAGGAG 
ATGTCCTACG GCACTCAGTC TACGTACGGT GGATGCAATT CGGCTGCAAA CAATAGCCAG 
CCGCACCTGC AGCAACCCAT CTGCGCCCCA TCCACCCAGC AGTTGGATCG CGAGCTAAAC 
GAGGCGGAGC AAATGAAGCT GCGGGAGCTG CGACTGGCCA GCGAGGCTCT TTATGATCCC 
GTGGACGAGG ACCTCAGCGC CCTGATGATG GGCGATGATC GCATTAAGCC CGACGACACT 
CGCCACAACC CAAAGCTATT GCAGCTGATC AATCTGACGG CGGTGGCCAT CAAGCGGCTT 
ATCAAAATGG CCAAGAAGAT TACAGCATTC CGTGACATGT GCCAGGAGGA CCAGGTGGCC 
CTACTCAAAG GTGGCTGCAC AGAAATGATG ATAATGCGCT CCGTAATGAT TTACGACGAC 
GATCGCGCCG CCTGGAAGGT ACCCCATACC AAAGAGAACA TGGGCAACAT ACGCACTGAC 
CTGCTCAAGT TTGCCGAAGG CAATATCTAC GAGGAGCACC AAAAGTTCAT CACAACGTTT 
GACGAGAAGT GGCGCATGGA CGAGAACATA ATCCTGATCA TGTGTGCCAT TGTCCTTTTT 
ACCTCGGCTC GATCGCGAGT GATACACAAA GACGTGATTA GATTGGAACA GAATTCCTAC 
TATTATCTTC TGCGAAGATA TCTGGAGAGT GTTTATTCTG GCTGTGAGGC GAGAAACGCG 
TTTATCAAGC TAATCCAAAA GATTTCAGAT GTGGAGCGTC TGAACAAGTT CATAATTAAT 
GTCTATTTGA ATGTTAACCC ATCCCAGGTG GAGCCCTTGC TGCGTGAAAT ATTCGATTTG 
AAAAATCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hr96 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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