Nmnat cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nmnat
Entrez Gene ID	42987
Full Name	Nicotinamide mononucleotide adenylyltransferase
Synonyms	3R4,AAL90149,CG13645,DmNMNAT,DmNmnat,Dmel\CG13645,NMNAT,Nmnat1,NmnatB,dNmnat,nmnat
General protein information	Preferred Names Nicotinamide mononucleotide adenylyltransferase Names CG13645 gene product from transcript CG13645-RA CG13645-PA CG13645-PB CG13645-PC Nmnat-PA Nmnat-PB Nmnat-PC nicotinamide mononucleotide adenyltransferase nicotinamide mononucleotide adenylyl transferase 1 nicotinamide mononucleotide adenylyltransferase nicotinamide mononucleotide adenylyltransferase 1 nicotinamide mononucleotide adenylytransferase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96B11-96B11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276013.1	NP_001262942.1	nicotinamide mononucleotide adenylyltransferase, isoform C
NM_001276013.2	NP_001262942.1	nicotinamide mononucleotide adenylyltransferase, isoform C
NM_001369970.1	NP_001356924.1	nicotinamide mononucleotide adenylyltransferase, isoform D
NM_170185.1	NP_733064.2	nicotinamide mononucleotide adenylyltransferase, isoform B
NM_143058.1	NP_651315.2	nicotinamide mononucleotide adenylyltransferase, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NMNAT3	XP_005634563.1
Gallus gallus (chicken)	NMNAT3	XP_004937092.1
Caenorhabditis elegans (roundworm)	W06B3.1	NP_510010.2
Magnaporthe oryzae (rice blast fungus)	MGG_01290	XP_003714213.1
Oryza sativa (rice)	Os02g0814900	NP_001048497.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009544	XP_310145.3
Bos taurus (cattle)	NMNAT3	XP_002685113.1
Drosophila melanogaster (fruit fly)	Nmnat	NP_733064.2
Mus musculus (house mouse)	Nmnat3	NP_653116.1
Danio rerio (zebrafish)	nmnat3	XP_700826.3
Caenorhabditis elegans (roundworm)	F26H9.4	NP_492480.1
Neurospora crassa	NCU04019	XP_957634.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thus axon injury seems to both turn on NP, but also dampen it by activating mitochondrial fission. We also identified caspases as negative regulators of axon injury-mediated NP, so mitochondrial fission could control NP through caspase activation. In addition to negative regulators of NP, we found that nicotinamide mononucleotide adenylyltransferase (Nmnat) is absolutely required for this type of NP.
Title: Mitochondria and Caspases Tune Nmnat-Mediated Stabilization to Promote Axon Regeneration.

Nmnat is alternatively spliced into two mRNA variants, RA and RB, which translate to protein isoforms with neuroprotective capacities.
Title: Alternative splicing of Drosophila Nmnat functions as a switch to enhance neuroprotection under stress.

Our studies reveal an essential neuroprotective role for endogenous Nmnat in hypoxia
Title: The role of autophagy in Nmnat-mediated protection against hypoxia-induced dendrite degeneration.

As a neuronal maintenance factor, NMNAT specifically maintains active zone structure by direct protein-protein interaction.
Title: Nicotinamide mononucleotide adenylyltransferase maintains active zone structure by stabilizing Bruchpilot.

An increased level of Nmnat protein in hiw mutants is both required and sufficient to inhibit axonal degeneration.
Title: The Highwire ubiquitin ligase promotes axonal degeneration by tuning levels of Nmnat protein.

The following Nmnat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nmnat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06051	NM_001276013.2 Latest version!	Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant C (Nmnat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa08781	NM_143058.1 Latest version!	Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant A (Nmnat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa08782	NM_170185.1 Latest version!	Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant B (Nmnat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa87706	NM_001369970.1 Latest version!	Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant D (Nmnat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OFa06051	NM_001276013.1	Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant C (Nmnat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nmnat ( NM_001276013.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262942.1 Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant C (Nmnat), mRNA.

Price & Availability↑

CloneID	OFa06051
Clone ID Related Accession (Same CDS sequence)	NM_001276013.1 , NM_001276013.2
Accession Version	NM_001276013.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-15
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinamide mononucleotide adenylyltransferase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.18 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAGCAT TCATCGAGGA AACAAAAAGC CTGCTGCCGC GGATCGCCTT CATAGCCTGC 
GGATGCTTCA GTCCACCCAC ACCCATGCAC CTTCGGATGT TCGAGATAGC CAAGGACCAC 
TTCGAAATGC AGGGAACCCA CAGAGTGGTA GGTGGCATCA TCTCGCCCAC GCACGACTCA 
TATGGCAAAA AGGGTCTGGC CTCCGCCTTG GACAGATGTG CCATGGTCAA GCTGGCCACC 
CAGAGCTCCA ATTGGATTCG GTTGTCCGAT TGGGAGGTGC ACCAGAATCA GTGGATGCGC 
ACGCAGGCGG TGCTCCAGCA CCACCAGAAC TATATCAATA ACCACATAAA TTCCGGCGGG 
GGAGGCGGAG ATGACGGGGA GAACACACAT CTACCCGGAT GGCTGCCTCG TGGGTTGCAC 
GACAGTCGGG ATCCTGTACA TCTGAAGCTA CTGTGCGGTG CCGATCTGCT GGAGTCCTTT 
GCTGTTCCAG GCCTATGGGC AGAGGCCGAT ATTGAGGACA TTGTGGCTAA CCATGGTTTG 
GTAGTTATCA CCCGGGCCGG CTCAAACCCA GGAAAATTCA TCTTTGACTC CGACATATTG 
ACCAAGTATC AGAGCAACAT TACGCTAATC ACCAACTGGG TGCCTAATGA GGTGAGCTCC 
ACGTTGATTC GGCGGCTCTT AGGACGCGGC CAGTCGGTCA AGTACCTTCT AGACGATCTG 
GTGCTGGAGT ACATCAAGCG CCAACGATTG TTTAACTTTA AATCTAAGTA TATAACGGAT 
GCGGTGCGTC CCAACCATTT GCTCTTTAAC CACGCCTATA CGGACAACAA CAAGAACGCG 
AACAGCTACT CTATAGGCGA CCAGCTGGAG CAGGACATGG ACGAGTCGGA CACTCCAAGC 
CCGCAACTGC AGCATACGCC TACGTCGCGG GTTTTCTGCT GCGGGGAGGT GCCTTTACGT 
GGATCCAAGG TGCTGCGATC CGGTCCTGGA CAAGCGGTGC AGGTCATCAC CATGCAGGCA 
GATGAGAAAG AGGAGAGCCA AGCAAAGAAG CAGAAGATTT CCCAAGTGCA ACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262942.1
CDS	42..1118
Translation	MSAFIEETKSLLPRIAFIACGCFSPPTPMHLRMFEIAKDHFEMQGTHRVVGGIISPTHDSYGKKGLASALDRCAMVKLATQSSNWIRLSDWEVHQNQWMRTQAVLQHHQNYINNHINSGGGGGDDGENTHLPGWLPRGLHDSRDPVHLKLLCGADLLESFAVPGLWAEADIEDIVANHGLVVITRAGSNPGKFIFDSDILTKYQSNITLITNWVPNEVSSTLIRRLLGRGQSVKYLLDDLVLEYIKRQRLFNFKSKYITDAVRPNHLLFNHAYTDNNKNANSYSIGDQLEQDMDESDTPSPQLQHTPTSRVFCCGEVPLRGSKVLRSGPGQAVQVITMQADEKEESQAKKQKISQVQL

Target ORF information:

RefSeq Version	NM_001276013.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant C (Nmnat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276013.1

ORF Insert Sequence:

ATGTCAGCAT TCATCGAGGA AACAAAAAGC CTGCTGCCGC GGATCGCCTT CATAGCCTGC 
GGATGCTTCA GTCCACCCAC ACCCATGCAC CTTCGGATGT TCGAGATAGC CAAGGACCAC 
TTCGAAATGC AGGGAACCCA CAGAGTGGTA GGTGGCATCA TCTCGCCCAC GCACGACTCA 
TATGGCAAAA AGGGTCTGGC CTCCGCCTTG GACAGATGTG CCATGGTCAA GCTGGCCACC 
CAGAGCTCCA ATTGGATTCG GTTGTCCGAT TGGGAGGTGC ACCAGAATCA GTGGATGCGC 
ACGCAGGCGG TGCTCCAGCA CCACCAGAAC TATATCAATA ACCACATAAA TTCCGGCGGG 
GGAGGCGGAG ATGACGGGGA GAACACACAT CTACCCGGAT GGCTGCCTCG TGGGTTGCAC 
GACAGTCGGG ATCCTGTACA TCTGAAGCTA CTGTGCGGTG CCGATCTGCT GGAGTCCTTT 
GCTGTTCCAG GCCTATGGGC AGAGGCCGAT ATTGAGGACA TTGTGGCTAA CCATGGTTTG 
GTAGTTATCA CCCGGGCCGG CTCAAACCCA GGAAAATTCA TCTTTGACTC CGACATATTG 
ACCAAGTATC AGAGCAACAT TACGCTAATC ACCAACTGGG TGCCTAATGA GGTGAGCTCC 
ACGTTGATTC GGCGGCTCTT AGGACGCGGC CAGTCGGTCA AGTACCTTCT AGACGATCTG 
GTGCTGGAGT ACATCAAGCG CCAACGATTG TTTAACTTTA AATCTAAGTA TATAACGGAT 
GCGGTGCGTC CCAACCATTT GCTCTTTAAC CACGCCTATA CGGACAACAA CAAGAACGCG 
AACAGCTACT CTATAGGCGA CCAGCTGGAG CAGGACATGG ACGAGTCGGA CACTCCAAGC 
CCGCAACTGC AGCATACGCC TACGTCGCGG GTTTTCTGCT GCGGGGAGGT GCCTTTACGT 
GGATCCAAGG TGCTGCGATC CGGTCCTGGA CAAGCGGTGC AGGTCATCAC CATGCAGGCA 
GATGAGAAAG AGGAGAGCCA AGCAAAGAAG CAGAAGATTT CCCAAGTGCA ACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nmnat ( NM_001276013.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262942.1 Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant C (Nmnat), mRNA.

Price & Availability↑

CloneID	OFa06051
Clone ID Related Accession (Same CDS sequence)	NM_001276013.1 , NM_001276013.2
Accession Version	NM_001276013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinamide mononucleotide adenylyltransferase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Apr 25, 2019 this sequence version replaced NM_001276013.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAGCAT TCATCGAGGA AACAAAAAGC CTGCTGCCGC GGATCGCCTT CATAGCCTGC 
GGATGCTTCA GTCCACCCAC ACCCATGCAC CTTCGGATGT TCGAGATAGC CAAGGACCAC 
TTCGAAATGC AGGGAACCCA CAGAGTGGTA GGTGGCATCA TCTCGCCCAC GCACGACTCA 
TATGGCAAAA AGGGTCTGGC CTCCGCCTTG GACAGATGTG CCATGGTCAA GCTGGCCACC 
CAGAGCTCCA ATTGGATTCG GTTGTCCGAT TGGGAGGTGC ACCAGAATCA GTGGATGCGC 
ACGCAGGCGG TGCTCCAGCA CCACCAGAAC TATATCAATA ACCACATAAA TTCCGGCGGG 
GGAGGCGGAG ATGACGGGGA GAACACACAT CTACCCGGAT GGCTGCCTCG TGGGTTGCAC 
GACAGTCGGG ATCCTGTACA TCTGAAGCTA CTGTGCGGTG CCGATCTGCT GGAGTCCTTT 
GCTGTTCCAG GCCTATGGGC AGAGGCCGAT ATTGAGGACA TTGTGGCTAA CCATGGTTTG 
GTAGTTATCA CCCGGGCCGG CTCAAACCCA GGAAAATTCA TCTTTGACTC CGACATATTG 
ACCAAGTATC AGAGCAACAT TACGCTAATC ACCAACTGGG TGCCTAATGA GGTGAGCTCC 
ACGTTGATTC GGCGGCTCTT AGGACGCGGC CAGTCGGTCA AGTACCTTCT AGACGATCTG 
GTGCTGGAGT ACATCAAGCG CCAACGATTG TTTAACTTTA AATCTAAGTA TATAACGGAT 
GCGGTGCGTC CCAACCATTT GCTCTTTAAC CACGCCTATA CGGACAACAA CAAGAACGCG 
AACAGCTACT CTATAGGCGA CCAGCTGGAG CAGGACATGG ACGAGTCGGA CACTCCAAGC 
CCGCAACTGC AGCATACGCC TACGTCGCGG GTTTTCTGCT GCGGGGAGGT GCCTTTACGT 
GGATCCAAGG TGCTGCGATC CGGTCCTGGA CAAGCGGTGC AGGTCATCAC CATGCAGGCA 
GATGAGAAAG AGGAGAGCCA AGCAAAGAAG CAGAAGATTT CCCAAGTGCA ACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262942.1
CDS	79..1155
Translation	MSAFIEETKSLLPRIAFIACGCFSPPTPMHLRMFEIAKDHFEMQGTHRVVGGIISPTHDSYGKKGLASALDRCAMVKLATQSSNWIRLSDWEVHQNQWMRTQAVLQHHQNYINNHINSGGGGGDDGENTHLPGWLPRGLHDSRDPVHLKLLCGADLLESFAVPGLWAEADIEDIVANHGLVVITRAGSNPGKFIFDSDILTKYQSNITLITNWVPNEVSSTLIRRLLGRGQSVKYLLDDLVLEYIKRQRLFNFKSKYITDAVRPNHLLFNHAYTDNNKNANSYSIGDQLEQDMDESDTPSPQLQHTPTSRVFCCGEVPLRGSKVLRSGPGQAVQVITMQADEKEESQAKKQKISQVQL

Target ORF information:

RefSeq Version	NM_001276013.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant C (Nmnat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276013.2

ORF Insert Sequence:

ATGTCAGCAT TCATCGAGGA AACAAAAAGC CTGCTGCCGC GGATCGCCTT CATAGCCTGC 
GGATGCTTCA GTCCACCCAC ACCCATGCAC CTTCGGATGT TCGAGATAGC CAAGGACCAC 
TTCGAAATGC AGGGAACCCA CAGAGTGGTA GGTGGCATCA TCTCGCCCAC GCACGACTCA 
TATGGCAAAA AGGGTCTGGC CTCCGCCTTG GACAGATGTG CCATGGTCAA GCTGGCCACC 
CAGAGCTCCA ATTGGATTCG GTTGTCCGAT TGGGAGGTGC ACCAGAATCA GTGGATGCGC 
ACGCAGGCGG TGCTCCAGCA CCACCAGAAC TATATCAATA ACCACATAAA TTCCGGCGGG 
GGAGGCGGAG ATGACGGGGA GAACACACAT CTACCCGGAT GGCTGCCTCG TGGGTTGCAC 
GACAGTCGGG ATCCTGTACA TCTGAAGCTA CTGTGCGGTG CCGATCTGCT GGAGTCCTTT 
GCTGTTCCAG GCCTATGGGC AGAGGCCGAT ATTGAGGACA TTGTGGCTAA CCATGGTTTG 
GTAGTTATCA CCCGGGCCGG CTCAAACCCA GGAAAATTCA TCTTTGACTC CGACATATTG 
ACCAAGTATC AGAGCAACAT TACGCTAATC ACCAACTGGG TGCCTAATGA GGTGAGCTCC 
ACGTTGATTC GGCGGCTCTT AGGACGCGGC CAGTCGGTCA AGTACCTTCT AGACGATCTG 
GTGCTGGAGT ACATCAAGCG CCAACGATTG TTTAACTTTA AATCTAAGTA TATAACGGAT 
GCGGTGCGTC CCAACCATTT GCTCTTTAAC CACGCCTATA CGGACAACAA CAAGAACGCG 
AACAGCTACT CTATAGGCGA CCAGCTGGAG CAGGACATGG ACGAGTCGGA CACTCCAAGC 
CCGCAACTGC AGCATACGCC TACGTCGCGG GTTTTCTGCT GCGGGGAGGT GCCTTTACGT 
GGATCCAAGG TGCTGCGATC CGGTCCTGGA CAAGCGGTGC AGGTCATCAC CATGCAGGCA 
GATGAGAAAG AGGAGAGCCA AGCAAAGAAG CAGAAGATTT CCCAAGTGCA ACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nmnat ( NM_143058.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651315.2 Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant A (Nmnat), mRNA.

Price & Availability↑

CloneID	OFa08781
Clone ID Related Accession (Same CDS sequence)	NM_143058.1
Accession Version	NM_143058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinamide mononucleotide adenylyltransferase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGTGA AAATCAGCTG GCCCAAGAAC AACATAACAA GCGAATGCTT CCGGCGGTTT 
GGATCTTTTA AAAGACGCTC AAAATCGAAG AAAATGTCAG CATTCATCGA GGAAACAAAA 
AGCCTGCTGC CGCGGATCGC CTTCATAGCC TGCGGATGCT TCAGTCCACC CACACCCATG 
CACCTTCGGA TGTTCGAGAT AGCCAAGGAC CACTTCGAAA TGCAGGGAAC CCACAGAGTG 
GTAGGTGGCA TCATCTCGCC CACGCACGAC TCATATGGCA AAAAGGGTCT GGCCTCCGCC 
TTGGACAGAT GTGCCATGGT CAAGCTGGCC ACCCAGAGCT CCAATTGGAT TCGGTTGTCC 
GATTGGGAGG TGCACCAGAA TCAGTGGATG CGCACGCAGG CGGTGCTCCA GCACCACCAG 
AACTATATCA ATAACCACAT AAATTCCGGC GGGGGAGGCG GAGATGACGG GGAGAACACA 
CATCTACCCG GATGGCTGCC TCGTGGGTTG CACGACAGTC GGGATCCTGT ACATCTGAAG 
CTACTGTGCG GTGCCGATCT GCTGGAGTCC TTTGCTGTTC CAGGCCTATG GGCAGAGGCC 
GATATTGAGG ACATTGTGGC TAACCATGGT TTGGTAGTTA TCACCCGGGC CGGCTCAAAC 
CCAGGAAAAT TCATCTTTGA CTCCGACATA TTGACCAAGT ATCAGAGCAA CATTACGCTA 
ATCACCAACT GGGTGCCTAA TGAGGTGAGC TCCACGTTGA TTCGGCGGCT CTTAGGACGC 
GGCCAGTCGG TCAAGTACCT TCTAGACGAT CTGGTGCTGG AGTACATCAA GCGCCAACGA 
TTGTTTAACT TTAAATCTAA GTATATAACG GATGCGGTGC GTCCCAACCA TTTGCTCTTT 
AACCACGCCT ATACGGACAA CAACAAGAAC GCGAACAGCT ACTCTATAGG CGACCAGCTG 
GAGCAGGACA TGGACGAGTC GGACACTCCA AGCCCGCAAC TGCAGCATAC GCCTACGTCG 
CGGGTTTTCT GCTGCGGGGA GGTGCCTTTA CGTGGATCCA AGGTGCTGCG ATCCGGTCCT 
GGACAAGCGG TGCAGGTCAT CACCATGCAG GCAGATGAGA AAGAGGAGAG CCAAGCAAAG 
AAGCAGAAGA TTTCCCAAGT GCAACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651315.2
CDS	22..1191
Translation	MIVKISWPKNNITSECFRRFGSFKRRSKSKKMSAFIEETKSLLPRIAFIACGCFSPPTPMHLRMFEIAKDHFEMQGTHRVVGGIISPTHDSYGKKGLASALDRCAMVKLATQSSNWIRLSDWEVHQNQWMRTQAVLQHHQNYINNHINSGGGGGDDGENTHLPGWLPRGLHDSRDPVHLKLLCGADLLESFAVPGLWAEADIEDIVANHGLVVITRAGSNPGKFIFDSDILTKYQSNITLITNWVPNEVSSTLIRRLLGRGQSVKYLLDDLVLEYIKRQRLFNFKSKYITDAVRPNHLLFNHAYTDNNKNANSYSIGDQLEQDMDESDTPSPQLQHTPTSRVFCCGEVPLRGSKVLRSGPGQAVQVITMQADEKEESQAKKQKISQVQL

Target ORF information:

RefSeq Version	NM_143058.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant A (Nmnat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143058.1

ORF Insert Sequence:

ATGATTGTGA AAATCAGCTG GCCCAAGAAC AACATAACAA GCGAATGCTT CCGGCGGTTT 
GGATCTTTTA AAAGACGCTC AAAATCGAAG AAAATGTCAG CATTCATCGA GGAAACAAAA 
AGCCTGCTGC CGCGGATCGC CTTCATAGCC TGCGGATGCT TCAGTCCACC CACACCCATG 
CACCTTCGGA TGTTCGAGAT AGCCAAGGAC CACTTCGAAA TGCAGGGAAC CCACAGAGTG 
GTAGGTGGCA TCATCTCGCC CACGCACGAC TCATATGGCA AAAAGGGTCT GGCCTCCGCC 
TTGGACAGAT GTGCCATGGT CAAGCTGGCC ACCCAGAGCT CCAATTGGAT TCGGTTGTCC 
GATTGGGAGG TGCACCAGAA TCAGTGGATG CGCACGCAGG CGGTGCTCCA GCACCACCAG 
AACTATATCA ATAACCACAT AAATTCCGGC GGGGGAGGCG GAGATGACGG GGAGAACACA 
CATCTACCCG GATGGCTGCC TCGTGGGTTG CACGACAGTC GGGATCCTGT ACATCTGAAG 
CTACTGTGCG GTGCCGATCT GCTGGAGTCC TTTGCTGTTC CAGGCCTATG GGCAGAGGCC 
GATATTGAGG ACATTGTGGC TAACCATGGT TTGGTAGTTA TCACCCGGGC CGGCTCAAAC 
CCAGGAAAAT TCATCTTTGA CTCCGACATA TTGACCAAGT ATCAGAGCAA CATTACGCTA 
ATCACCAACT GGGTGCCTAA TGAGGTGAGC TCCACGTTGA TTCGGCGGCT CTTAGGACGC 
GGCCAGTCGG TCAAGTACCT TCTAGACGAT CTGGTGCTGG AGTACATCAA GCGCCAACGA 
TTGTTTAACT TTAAATCTAA GTATATAACG GATGCGGTGC GTCCCAACCA TTTGCTCTTT 
AACCACGCCT ATACGGACAA CAACAAGAAC GCGAACAGCT ACTCTATAGG CGACCAGCTG 
GAGCAGGACA TGGACGAGTC GGACACTCCA AGCCCGCAAC TGCAGCATAC GCCTACGTCG 
CGGGTTTTCT GCTGCGGGGA GGTGCCTTTA CGTGGATCCA AGGTGCTGCG ATCCGGTCCT 
GGACAAGCGG TGCAGGTCAT CACCATGCAG GCAGATGAGA AAGAGGAGAG CCAAGCAAAG 
AAGCAGAAGA TTTCCCAAGT GCAACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nmnat ( NM_170185.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733064.2 Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant B (Nmnat), mRNA.

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CloneID	OFa08782
Clone ID Related Accession (Same CDS sequence)	NM_170185.1
Accession Version	NM_170185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinamide mononucleotide adenylyltransferase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGTGA AAATCAGCTG GCCCAAGAAC AACATAACAA GCGAATGCTT CCGGCGGTTT 
GGATCTTTTA AAAGACGCTC AAAATCGAAG AAAATGTCAG CATTCATCGA GGAAACAAAA 
AGCCTGCTGC CGCGGATCGC CTTCATAGCC TGCGGATGCT TCAGTCCACC CACACCCATG 
CACCTTCGGA TGTTCGAGAT AGCCAAGGAC CACTTCGAAA TGCAGGGAAC CCACAGAGTG 
GTAGGTGGCA TCATCTCGCC CACGCACGAC TCATATGGCA AAAAGGGTCT GGCCTCCGCC 
TTGGACAGAT GTGCCATGGT CAAGCTGGCC ACCCAGAGCT CCAATTGGAT TCGGTTGTCC 
GATTGGGAGG TGCACCAGAA TCAGTGGATG CGCACGCAGG CGGTGCTCCA GCACCACCAG 
AACTATATCA ATAACCACAT AAATTCCGGC GGGGGAGGCG GAGATGACGG GGAGAACACA 
CATCTACCCG GATGGCTGCC TCGTGGGTTG CACGACAGTC GGGATCCTGT ACATCTGAAG 
CTACTGTGCG GTGCCGATCT GCTGGAGTCC TTTGCTGTTC CAGGCCTATG GGCAGAGGCC 
GATATTGAGG ACATTGTGGC TAACCATGGT TTGGTAGTTA TCACCCGGGC CGGCTCAAAC 
CCAGGAAAAT TCATCTTTGA CTCCGACATA TTGACCAAGT ATCAGAGCAA CATTACGCTA 
ATCACCAACT GGGTGCCTAA TGAGGTGAGC TCCACGTTGA TTCGGCGGCT CTTAGGACGC 
GGCCAGTCGG TCAAGTACCT TCTAGACGAT CTGGTGCTGG AGTACATCAA GCGCCAACGA 
TTGTTTAACT TTAAATCGCG GGATGCACCG GCTCCGACCG AATGCGACTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733064.2
CDS	22..915
Translation	MIVKISWPKNNITSECFRRFGSFKRRSKSKKMSAFIEETKSLLPRIAFIACGCFSPPTPMHLRMFEIAKDHFEMQGTHRVVGGIISPTHDSYGKKGLASALDRCAMVKLATQSSNWIRLSDWEVHQNQWMRTQAVLQHHQNYINNHINSGGGGGDDGENTHLPGWLPRGLHDSRDPVHLKLLCGADLLESFAVPGLWAEADIEDIVANHGLVVITRAGSNPGKFIFDSDILTKYQSNITLITNWVPNEVSSTLIRRLLGRGQSVKYLLDDLVLEYIKRQRLFNFKSRDAPAPTECDS

Target ORF information:

RefSeq Version	NM_170185.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant B (Nmnat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170185.1

ORF Insert Sequence:

ATGATTGTGA AAATCAGCTG GCCCAAGAAC AACATAACAA GCGAATGCTT CCGGCGGTTT 
GGATCTTTTA AAAGACGCTC AAAATCGAAG AAAATGTCAG CATTCATCGA GGAAACAAAA 
AGCCTGCTGC CGCGGATCGC CTTCATAGCC TGCGGATGCT TCAGTCCACC CACACCCATG 
CACCTTCGGA TGTTCGAGAT AGCCAAGGAC CACTTCGAAA TGCAGGGAAC CCACAGAGTG 
GTAGGTGGCA TCATCTCGCC CACGCACGAC TCATATGGCA AAAAGGGTCT GGCCTCCGCC 
TTGGACAGAT GTGCCATGGT CAAGCTGGCC ACCCAGAGCT CCAATTGGAT TCGGTTGTCC 
GATTGGGAGG TGCACCAGAA TCAGTGGATG CGCACGCAGG CGGTGCTCCA GCACCACCAG 
AACTATATCA ATAACCACAT AAATTCCGGC GGGGGAGGCG GAGATGACGG GGAGAACACA 
CATCTACCCG GATGGCTGCC TCGTGGGTTG CACGACAGTC GGGATCCTGT ACATCTGAAG 
CTACTGTGCG GTGCCGATCT GCTGGAGTCC TTTGCTGTTC CAGGCCTATG GGCAGAGGCC 
GATATTGAGG ACATTGTGGC TAACCATGGT TTGGTAGTTA TCACCCGGGC CGGCTCAAAC 
CCAGGAAAAT TCATCTTTGA CTCCGACATA TTGACCAAGT ATCAGAGCAA CATTACGCTA 
ATCACCAACT GGGTGCCTAA TGAGGTGAGC TCCACGTTGA TTCGGCGGCT CTTAGGACGC 
GGCCAGTCGG TCAAGTACCT TCTAGACGAT CTGGTGCTGG AGTACATCAA GCGCCAACGA 
TTGTTTAACT TTAAATCGCG GGATGCACCG GCTCCGACCG AATGCGACTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nmnat ( NM_001369970.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356924.1 Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant D (Nmnat), mRNA.

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CloneID	OFa87706
Clone ID Related Accession (Same CDS sequence)	NM_001369970.1
Accession Version	NM_001369970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinamide mononucleotide adenylyltransferase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAGCAT TCATCGAGGA AACAAAAAGC CTGCTGCCGC GGATCGCCTT CATAGCCTGC 
GGATGCTTCA GTCCACCCAC ACCCATGCAC CTTCGGATGT TCGAGATAGC CAAGGACCAC 
TTCGAAATGC AGGGAACCCA CAGAGTGGTA GGTGGCATCA TCTCGCCCAC GCACGACTCA 
TATGGCAAAA AGGGTCTGGC CTCCGCCTTG GACAGATGTG CCATGGTCAA GCTGGCCACC 
CAGAGCTCCA ATTGGATTCG GTTGTCCGAT TGGGAGGTGC ACCAGAATCA GTGGATGCGC 
ACGCAGGCGG TGCTCCAGCA CCACCAGAAC TATATCAATA ACCACATAAA TTCCGGCGGG 
GGAGGCGGAG ATGACGGGGA GAACACACAT CTACCCGGAT GGCTGCCTCG TGGGTTGCAC 
GACAGTCGGG ATCCTGTACA TCTGAAGCTA CTGTGCGGTG CCGATCTGCT GGAGTCCTTT 
GCTGTTCCAG GCCTATGGGC AGAGGCCGAT ATTGAGGACA TTGTGGCTAA CCATGGTTTG 
GTAGTTATCA CCCGGGCCGG CTCAAACCCA GGAAAATTCA TCTTTGACTC CGACATATTG 
ACCAAGTATC AGAGCAACAT TACGCTAATC ACCAACTGGG TGCCTAATGA GGTGAGCTCC 
ACGTTGATTC GGCGGCTCTT AGGACGCGGC CAGTCGGTCA AGTACCTTCT AGACGATCTG 
GTGCTGGAGT ACATCAAGCG CCAACGATTG TTTAACTTTA AATCGCGGGA TGCACCGGCT 
CCGACCGAAT GCGACTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356924.1
CDS	79..879
Translation	MSAFIEETKSLLPRIAFIACGCFSPPTPMHLRMFEIAKDHFEMQGTHRVVGGIISPTHDSYGKKGLASALDRCAMVKLATQSSNWIRLSDWEVHQNQWMRTQAVLQHHQNYINNHINSGGGGGDDGENTHLPGWLPRGLHDSRDPVHLKLLCGADLLESFAVPGLWAEADIEDIVANHGLVVITRAGSNPGKFIFDSDILTKYQSNITLITNWVPNEVSSTLIRRLLGRGQSVKYLLDDLVLEYIKRQRLFNFKSRDAPAPTECDS

Target ORF information:

RefSeq Version	NM_001369970.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinamide mononucleotide adenylyltransferase, transcript variant D (Nmnat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369970.1

ORF Insert Sequence:

ATGTCAGCAT TCATCGAGGA AACAAAAAGC CTGCTGCCGC GGATCGCCTT CATAGCCTGC 
GGATGCTTCA GTCCACCCAC ACCCATGCAC CTTCGGATGT TCGAGATAGC CAAGGACCAC 
TTCGAAATGC AGGGAACCCA CAGAGTGGTA GGTGGCATCA TCTCGCCCAC GCACGACTCA 
TATGGCAAAA AGGGTCTGGC CTCCGCCTTG GACAGATGTG CCATGGTCAA GCTGGCCACC 
CAGAGCTCCA ATTGGATTCG GTTGTCCGAT TGGGAGGTGC ACCAGAATCA GTGGATGCGC 
ACGCAGGCGG TGCTCCAGCA CCACCAGAAC TATATCAATA ACCACATAAA TTCCGGCGGG 
GGAGGCGGAG ATGACGGGGA GAACACACAT CTACCCGGAT GGCTGCCTCG TGGGTTGCAC 
GACAGTCGGG ATCCTGTACA TCTGAAGCTA CTGTGCGGTG CCGATCTGCT GGAGTCCTTT 
GCTGTTCCAG GCCTATGGGC AGAGGCCGAT ATTGAGGACA TTGTGGCTAA CCATGGTTTG 
GTAGTTATCA CCCGGGCCGG CTCAAACCCA GGAAAATTCA TCTTTGACTC CGACATATTG 
ACCAAGTATC AGAGCAACAT TACGCTAATC ACCAACTGGG TGCCTAATGA GGTGAGCTCC 
ACGTTGATTC GGCGGCTCTT AGGACGCGGC CAGTCGGTCA AGTACCTTCT AGACGATCTG 
GTGCTGGAGT ACATCAAGCG CCAACGATTG TTTAACTTTA AATCGCGGGA TGCACCGGCT 
CCGACCGAAT GCGACTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nmnat cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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