ash2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ash2
Entrez Gene ID	42936
Full Name	absent, small, or homeotic discs 2
Synonyms	1124/11,291.8,703,ASH2,Ash2,CG6677,Dmel\CG6677,ash-2,l(3)112411,l(3)S112411,l(3)SG65,mad
General protein information	Preferred Names absent, small, or homeotic discs 2 Names CG6677 gene product from transcript CG6677-RC CG6677-PB CG6677-PC CG6677-PE absent small or homeotic discs 2 absent, small or homeotic discs 2 ash2-PB ash2-PC ash2-PE many abnormal discs
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96A13-96A13\|3-78.6 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260363.1	NP_001247292.1	absent, small, or homeotic discs 2, isoform E
NM_176558.3	NP_788735.1	absent, small, or homeotic discs 2, isoform C
NM_170160.2	NP_733024.1	absent, small, or homeotic discs 2, isoform B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ASH2L	NP_001026566.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003705	XP_562582.3
Schizosaccharomyces pombe (fission yeast)	ash2	NP_596557.1
Mus musculus (house mouse)	Ash2l	NP_035921.2
Danio rerio (zebrafish)	ash2l	NP_001103575.2
Drosophila melanogaster (fruit fly)	ash2	NP_788735.1
Pan troglodytes (chimpanzee)	ASH2L	XP_001170172.1
	ASH2L	XP_002805359.1
Canis lupus familiaris (dog)	ASH2L	XP_005629916.1
Rattus norvegicus (Norway rat)	Ash2l	NP_001099559.1
Xenopus tropicalis (tropical clawed frog)	ash2l	XP_002932768.2
Homo sapiens (human)	ASH2L	NP_004665.2
Bos taurus (cattle)	ASH2L	NP_001192402.1
Caenorhabditis elegans (roundworm)	ash-2	NP_496553.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ash2 functions together with Trr as an ecdysone receptor coactivator.
Title: Ash2 acts as an ecdysone receptor coactivator by stabilizing the histone methyltransferase Trr.

Provides a molecular framework for a mechanism required to maintain cellular identities in wing development.
Title: Activation and repression activities of ash2 in Drosophila wing imaginal discs.

ASH2 maintains active transcription by binding a producer of nuclear phosphoinositides and downregulating histone H1 hyperphosphorylation.
Title: The direct interaction between ASH2, a Drosophila trithorax group protein, and SKTL, a nuclear phosphatidylinositol 4-phosphate 5-kinase, implies a role for phosphatidylinositol 4,5-bisphosphate in maintaining transcriptionally active chromatin.

transcriptional network controlled by the trithorax-group gene ash2
Title: Transcriptional network controlled by the trithorax-group gene ash2 in Drosophila melanogaster.

the gene is involved in Drosophila wing development
Title: The ash2 gene is involved in Drosophila wing development.

The following ash2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ash2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05980	NM_176558.3 Latest version!	Drosophila melanogaster absent, small, or homeotic discs 2 (ash2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05981	NM_170160.2 Latest version!	Drosophila melanogaster absent, small, or homeotic discs 2 (ash2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07744	NM_001260363.1 Latest version!	Drosophila melanogaster absent, small, or homeotic discs 2 (ash2), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ash2 ( NM_176558.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788735.1 Drosophila melanogaster absent, small, or homeotic discs 2 (ash2), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05980
Clone ID Related Accession (Same CDS sequence)	NM_176558.3
Accession Version	NM_176558.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	absent, small, or homeotic discs 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_176558.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGACA GCCAAATGGA CACAAGCTCG CCGACAGAGT CCAGCTCCGA GGTGAACTTC 
ACTGCGGAGG AGGATAAATC GCAGGAAACG CGTTCCGCAG CCGGCGTTTG TTACTGCGGC 
AAGGAGCGCA ATCTCAATAT TGTGGAGCTG CTGTGTGCCA CCTGTTCACG CTGGGTGCAC 
GAGACCTGCG TTTCCTACCA GCTTGGTAAG GGTAAACTTC TGCCCTTCAT CACCAACTAT 
GTGTTCGTCT GTAAAAATTG CTCTGCCAGC GGATTGGAGA GCTTTCGCAA GAGCCAGGCT 
ACCATATCCC AGATGTGCCA CTGTGCCATT GCCAACATGC AGCAGGCGGC TTCCAGGGAC 
GGACGTCGCC AGATTCAGTT TAGCAAGGAC AAGGAGATCA TACCGTACAT CGAACAGTAC 
TGGGAGGCCA TGACCACCAT GCCACGCAGA CTCACCCAGT CCTGGTACAG CACCGTCCAG 
CGATCTTTGG TCAAGGATGT GCAGACCCTG TTCACCTACG AGGAGCATGC GGAACATGGC 
GCCATGTACG GACTATTCCA TCAGGATCTG CGAATCATCA AGCCCAACTA CGAGAGCATG 
AGCAAGAGCG GCGCCCTGCG ACTCACCGAC GATGGATATA CTCAAGCTTC CCTCTCCAAA 
AACAATCGAC AGAAAAGGAA ATTTCCCGGC ACGGATTCGG GTCCCACGGG CAAGAAGGGT 
CGGCCCAGTT CCGATATTAC GGCCAATGTA AAGTTGCCAC CGCATGGCTA TCCATTGGAA 
CACCCCTTCA ACAAGGATGG CTATCGTTAT ATACTCGCCG AACCGGATCC ACATGCTCCA 
TTTCGTCAGG AGTTCGACGA GAGCTCCGAT TGGGCTGGCA AACCTATCCC CGGCTGGCTC 
TACCGCATCC TGGTGCCACA TTCTGTGCTC CTGGCGCTGC ATGATCGGGC ACCACAGCTG 
AAAATAAGCG AGGATCGGTT GGCGGTGACG GGCGAACGTG GTTACTGCAT GGTCCGAGCC 
ACACACTCTG TGAACCGGGG ATGCTGGTAC TTTGAGGTCA CCATCGAAGA GATGCCCGAC 
GGAGCTGCCA CGCGACTTGG CTGGGGCCGG GAGTACGGCA ACTTGCAGGC TCCATTGGGA 
TACGACAAGT TCGGTTACTC CTGGAGATCT CGCAAGGGCA CCAAGTTTAC CGAGAGCCAT 
GGCAAACACT ACAGTGATGC CTATGTGGAG GGCGATACAT TGGGATTCCT CATAGAGCTG 
CCAGAGGAGG CGTCGCTCGA CTATCTGCCC AACACATTCA AAGATCGGCC CCTGGTCAAG 
TTCAAGTCTC ATCTGTACTA CGAGGATAAG GACAAGATCA CAGAAACCCT GAAAAATCTG 
CACATCCTGC AGGGCAGCCG CATCGAGTTC TTTAAGAACG GTCAATCGCA GGGTGTGGCA 
TTCGAAGACA TTTATGCCGG CAGCTATTTC CCGGCCATCT CGATCCACAA AAGTGCAACG 
GTCAGCGTAA ACTTCGGACC CGCCTTCAAG TATCCCGAGG TGCTCGTCGA GCACAAAGCC 
AAGGGGATGC ACGATCGCGT GGAGGAGCTG ATCACAGAGC AATGTTTAGC CGACACCCTC 
TACCTCACAG AACACGATGG ACGTCTGCGC TTGGATAATA TGGGTCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788735.1
CDS	105..1775
Translation	MEDSQMDTSSPTESSSEVNFTAEEDKSQETRSAAGVCYCGKERNLNIVELLCATCSRWVHETCVSYQLGKGKLLPFITNYVFVCKNCSASGLESFRKSQATISQMCHCAIANMQQAASRDGRRQIQFSKDKEIIPYIEQYWEAMTTMPRRLTQSWYSTVQRSLVKDVQTLFTYEEHAEHGAMYGLFHQDLRIIKPNYESMSKSGALRLTDDGYTQASLSKNNRQKRKFPGTDSGPTGKKGRPSSDITANVKLPPHGYPLEHPFNKDGYRYILAEPDPHAPFRQEFDESSDWAGKPIPGWLYRILVPHSVLLALHDRAPQLKISEDRLAVTGERGYCMVRATHSVNRGCWYFEVTIEEMPDGAATRLGWGREYGNLQAPLGYDKFGYSWRSRKGTKFTESHGKHYSDAYVEGDTLGFLIELPEEASLDYLPNTFKDRPLVKFKSHLYYEDKDKITETLKNLHILQGSRIEFFKNGQSQGVAFEDIYAGSYFPAISIHKSATVSVNFGPAFKYPEVLVEHKAKGMHDRVEELITEQCLADTLYLTEHDGRLRLDNMGL

Target ORF information:

RefSeq Version	NM_176558.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster absent, small, or homeotic discs 2 (ash2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176558.3

ORF Insert Sequence:

ATGGAGGACA GCCAAATGGA CACAAGCTCG CCGACAGAGT CCAGCTCCGA GGTGAACTTC 
ACTGCGGAGG AGGATAAATC GCAGGAAACG CGTTCCGCAG CCGGCGTTTG TTACTGCGGC 
AAGGAGCGCA ATCTCAATAT TGTGGAGCTG CTGTGTGCCA CCTGTTCACG CTGGGTGCAC 
GAGACCTGCG TTTCCTACCA GCTTGGTAAG GGTAAACTTC TGCCCTTCAT CACCAACTAT 
GTGTTCGTCT GTAAAAATTG CTCTGCCAGC GGATTGGAGA GCTTTCGCAA GAGCCAGGCT 
ACCATATCCC AGATGTGCCA CTGTGCCATT GCCAACATGC AGCAGGCGGC TTCCAGGGAC 
GGACGTCGCC AGATTCAGTT TAGCAAGGAC AAGGAGATCA TACCGTACAT CGAACAGTAC 
TGGGAGGCCA TGACCACCAT GCCACGCAGA CTCACCCAGT CCTGGTACAG CACCGTCCAG 
CGATCTTTGG TCAAGGATGT GCAGACCCTG TTCACCTACG AGGAGCATGC GGAACATGGC 
GCCATGTACG GACTATTCCA TCAGGATCTG CGAATCATCA AGCCCAACTA CGAGAGCATG 
AGCAAGAGCG GCGCCCTGCG ACTCACCGAC GATGGATATA CTCAAGCTTC CCTCTCCAAA 
AACAATCGAC AGAAAAGGAA ATTTCCCGGC ACGGATTCGG GTCCCACGGG CAAGAAGGGT 
CGGCCCAGTT CCGATATTAC GGCCAATGTA AAGTTGCCAC CGCATGGCTA TCCATTGGAA 
CACCCCTTCA ACAAGGATGG CTATCGTTAT ATACTCGCCG AACCGGATCC ACATGCTCCA 
TTTCGTCAGG AGTTCGACGA GAGCTCCGAT TGGGCTGGCA AACCTATCCC CGGCTGGCTC 
TACCGCATCC TGGTGCCACA TTCTGTGCTC CTGGCGCTGC ATGATCGGGC ACCACAGCTG 
AAAATAAGCG AGGATCGGTT GGCGGTGACG GGCGAACGTG GTTACTGCAT GGTCCGAGCC 
ACACACTCTG TGAACCGGGG ATGCTGGTAC TTTGAGGTCA CCATCGAAGA GATGCCCGAC 
GGAGCTGCCA CGCGACTTGG CTGGGGCCGG GAGTACGGCA ACTTGCAGGC TCCATTGGGA 
TACGACAAGT TCGGTTACTC CTGGAGATCT CGCAAGGGCA CCAAGTTTAC CGAGAGCCAT 
GGCAAACACT ACAGTGATGC CTATGTGGAG GGCGATACAT TGGGATTCCT CATAGAGCTG 
CCAGAGGAGG CGTCGCTCGA CTATCTGCCC AACACATTCA AAGATCGGCC CCTGGTCAAG 
TTCAAGTCTC ATCTGTACTA CGAGGATAAG GACAAGATCA CAGAAACCCT GAAAAATCTG 
CACATCCTGC AGGGCAGCCG CATCGAGTTC TTTAAGAACG GTCAATCGCA GGGTGTGGCA 
TTCGAAGACA TTTATGCCGG CAGCTATTTC CCGGCCATCT CGATCCACAA AAGTGCAACG 
GTCAGCGTAA ACTTCGGACC CGCCTTCAAG TATCCCGAGG TGCTCGTCGA GCACAAAGCC 
AAGGGGATGC ACGATCGCGT GGAGGAGCTG ATCACAGAGC AATGTTTAGC CGACACCCTC 
TACCTCACAG AACACGATGG ACGTCTGCGC TTGGATAATA TGGGTCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash2 ( NM_170160.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733024.1 Drosophila melanogaster absent, small, or homeotic discs 2 (ash2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05981
Clone ID Related Accession (Same CDS sequence)	NM_170160.2
Accession Version	NM_170160.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	absent, small, or homeotic discs 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_170160.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTCAT CTTTTACGGA CGAGGAGTCT TCCCTCTCCA AAAACAATCG ACAGAAAAGG 
AAATTTCCCG GCACGGATTC GGGTCCCACG GGCAAGAAGG GTCGGCCCAG TTCCGATATT 
ACGGCCAATG TAAAGTTGCC ACCGCATGGC TATCCATTGG AACACCCCTT CAACAAGGAT 
GGCTATCGTT ATATACTCGC CGAACCGGAT CCACATGCTC CATTTCGTCA GGAGTTCGAC 
GAGAGCTCCG ATTGGGCTGG CAAACCTATC CCCGGCTGGC TCTACCGCAT CCTGGTGCCA 
CATTCTGTGC TCCTGGCGCT GCATGATCGG GCACCACAGC TGAAAATAAG CGAGGATCGG 
TTGGCGGTGA CGGGCGAACG TGGTTACTGC ATGGTCCGAG CCACACACTC TGTGAACCGG 
GGATGCTGGT ACTTTGAGGT CACCATCGAA GAGATGCCCG ACGGAGCTGC CACGCGACTT 
GGCTGGGGCC GGGAGTACGG CAACTTGCAG GCTCCATTGG GATACGACAA GTTCGGTTAC 
TCCTGGAGAT CTCGCAAGGG CACCAAGTTT ACCGAGAGCC ATGGCAAACA CTACAGTGAT 
GCCTATGTGG AGGGCGATAC ATTGGGATTC CTCATAGAGC TGCCAGAGGA GGCGTCGCTC 
GACTATCTGC CCAACACATT CAAAGATCGG CCCCTGGTCA AGTTCAAGTC TCATCTGTAC 
TACGAGGATA AGGACAAGAT CACAGAAACC CTGAAAAATC TGCACATCCT GCAGGGCAGC 
CGCATCGAGT TCTTTAAGAA CGGTCAATCG CAGGGTGTGG CATTCGAAGA CATTTATGCC 
GGCAGCTATT TCCCGGCCAT CTCGATCCAC AAAAGTGCAA CGGTCAGCGT AAACTTCGGA 
CCCGCCTTCA AGTATCCCGA GGTGCTCGTC GAGCACAAAG CCAAGGGGAT GCACGATCGC 
GTGGAGGAGC TGATCACAGA GCAATGTTTA GCCGACACCC TCTACCTCAC AGAACACGAT 
GGACGTCTGC GCTTGGATAA TATGGGTCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733024.1
CDS	187..1239
Translation	MASSFTDEESSLSKNNRQKRKFPGTDSGPTGKKGRPSSDITANVKLPPHGYPLEHPFNKDGYRYILAEPDPHAPFRQEFDESSDWAGKPIPGWLYRILVPHSVLLALHDRAPQLKISEDRLAVTGERGYCMVRATHSVNRGCWYFEVTIEEMPDGAATRLGWGREYGNLQAPLGYDKFGYSWRSRKGTKFTESHGKHYSDAYVEGDTLGFLIELPEEASLDYLPNTFKDRPLVKFKSHLYYEDKDKITETLKNLHILQGSRIEFFKNGQSQGVAFEDIYAGSYFPAISIHKSATVSVNFGPAFKYPEVLVEHKAKGMHDRVEELITEQCLADTLYLTEHDGRLRLDNMGL

Target ORF information:

RefSeq Version	NM_170160.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster absent, small, or homeotic discs 2 (ash2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170160.2

ORF Insert Sequence:

ATGGCGTCAT CTTTTACGGA CGAGGAGTCT TCCCTCTCCA AAAACAATCG ACAGAAAAGG 
AAATTTCCCG GCACGGATTC GGGTCCCACG GGCAAGAAGG GTCGGCCCAG TTCCGATATT 
ACGGCCAATG TAAAGTTGCC ACCGCATGGC TATCCATTGG AACACCCCTT CAACAAGGAT 
GGCTATCGTT ATATACTCGC CGAACCGGAT CCACATGCTC CATTTCGTCA GGAGTTCGAC 
GAGAGCTCCG ATTGGGCTGG CAAACCTATC CCCGGCTGGC TCTACCGCAT CCTGGTGCCA 
CATTCTGTGC TCCTGGCGCT GCATGATCGG GCACCACAGC TGAAAATAAG CGAGGATCGG 
TTGGCGGTGA CGGGCGAACG TGGTTACTGC ATGGTCCGAG CCACACACTC TGTGAACCGG 
GGATGCTGGT ACTTTGAGGT CACCATCGAA GAGATGCCCG ACGGAGCTGC CACGCGACTT 
GGCTGGGGCC GGGAGTACGG CAACTTGCAG GCTCCATTGG GATACGACAA GTTCGGTTAC 
TCCTGGAGAT CTCGCAAGGG CACCAAGTTT ACCGAGAGCC ATGGCAAACA CTACAGTGAT 
GCCTATGTGG AGGGCGATAC ATTGGGATTC CTCATAGAGC TGCCAGAGGA GGCGTCGCTC 
GACTATCTGC CCAACACATT CAAAGATCGG CCCCTGGTCA AGTTCAAGTC TCATCTGTAC 
TACGAGGATA AGGACAAGAT CACAGAAACC CTGAAAAATC TGCACATCCT GCAGGGCAGC 
CGCATCGAGT TCTTTAAGAA CGGTCAATCG CAGGGTGTGG CATTCGAAGA CATTTATGCC 
GGCAGCTATT TCCCGGCCAT CTCGATCCAC AAAAGTGCAA CGGTCAGCGT AAACTTCGGA 
CCCGCCTTCA AGTATCCCGA GGTGCTCGTC GAGCACAAAG CCAAGGGGAT GCACGATCGC 
GTGGAGGAGC TGATCACAGA GCAATGTTTA GCCGACACCC TCTACCTCAC AGAACACGAT 
GGACGTCTGC GCTTGGATAA TATGGGTCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash2 ( NM_001260363.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247292.1 Drosophila melanogaster absent, small, or homeotic discs 2 (ash2), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa07744
Clone ID Related Accession (Same CDS sequence)	NM_001260363.1
Accession Version	NM_001260363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	absent, small, or homeotic discs 2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCCGAG CCACACACTC TGTGAACCGG GGATGCTGGT ACTTTGAGGT CACCATCGAA 
GAGATGCCCG ACGGAGCTGC CACGCGACTT GGCTGGGGCC GGGAGTACGG CAACTTGCAG 
GCTCCATTGG GATACGACAA GTTCGGTTAC TCCTGGAGAT CTCGCAAGGG CACCAAGTTT 
ACCGAGAGCC ATGGCAAACA CTACAGTGAT GCCTATGTGG AGGGCGATAC ATTGGGATTC 
CTCATAGAGC TGCCAGAGGA GGCGTCGCTC GACTATCTGC CCAACACATT CAAAGATCGG 
CCCCTGGTCA AGTTCAAGTC TCATCTGTAC TACGAGGATA AGGACAAGAT CACAGAAACC 
CTGAAAAATC TGCACATCCT GCAGGGCAGC CGCATCGAGT TCTTTAAGAA CGGTCAATCG 
CAGGGTGTGG CATTCGAAGA CATTTATGCC GGCAGCTATT TCCCGGCCAT CTCGATCCAC 
AAAAGTGCAA CGGTCAGCGT AAACTTCGGA CCCGCCTTCA AGTATCCCGA GGTGCTCGTC 
GAGCACAAAG CCAAGGGGAT GCACGATCGC GTGGAGGAGC TGATCACAGA GCAATGTTTA 
GCCGACACCC TCTACCTCAC AGAACACGAT GGACGTCTGC GCTTGGATAA TATGGGTCTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247292.1
CDS	435..1097
Translation	MVRATHSVNRGCWYFEVTIEEMPDGAATRLGWGREYGNLQAPLGYDKFGYSWRSRKGTKFTESHGKHYSDAYVEGDTLGFLIELPEEASLDYLPNTFKDRPLVKFKSHLYYEDKDKITETLKNLHILQGSRIEFFKNGQSQGVAFEDIYAGSYFPAISIHKSATVSVNFGPAFKYPEVLVEHKAKGMHDRVEELITEQCLADTLYLTEHDGRLRLDNMGL

Target ORF information:

RefSeq Version	NM_001260363.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster absent, small, or homeotic discs 2 (ash2), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260363.1

ORF Insert Sequence:

ATGGTCCGAG CCACACACTC TGTGAACCGG GGATGCTGGT ACTTTGAGGT CACCATCGAA 
GAGATGCCCG ACGGAGCTGC CACGCGACTT GGCTGGGGCC GGGAGTACGG CAACTTGCAG 
GCTCCATTGG GATACGACAA GTTCGGTTAC TCCTGGAGAT CTCGCAAGGG CACCAAGTTT 
ACCGAGAGCC ATGGCAAACA CTACAGTGAT GCCTATGTGG AGGGCGATAC ATTGGGATTC 
CTCATAGAGC TGCCAGAGGA GGCGTCGCTC GACTATCTGC CCAACACATT CAAAGATCGG 
CCCCTGGTCA AGTTCAAGTC TCATCTGTAC TACGAGGATA AGGACAAGAT CACAGAAACC 
CTGAAAAATC TGCACATCCT GCAGGGCAGC CGCATCGAGT TCTTTAAGAA CGGTCAATCG 
CAGGGTGTGG CATTCGAAGA CATTTATGCC GGCAGCTATT TCCCGGCCAT CTCGATCCAC 
AAAAGTGCAA CGGTCAGCGT AAACTTCGGA CCCGCCTTCA AGTATCCCGA GGTGCTCGTC 
GAGCACAAAG CCAAGGGGAT GCACGATCGC GTGGAGGAGC TGATCACAGA GCAATGTTTA 
GCCGACACCC TCTACCTCAC AGAACACGAT GGACGTCTGC GCTTGGATAA TATGGGTCTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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ash2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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