atl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	atl
Entrez Gene ID	42934
Full Name	atlastin
Synonyms	ATL,Atl,CG6668,Dmel\CG6668
General protein information	Preferred Names atlastin Names CG6668 gene product from transcript CG6668-RA CG6668-PA CG6668-PB CG6668-PC D-atlastin atl-PA atl-PB atl-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96A13-96A13

mRNA and Protein(s)

mRNA	Protein	Name
NM_143017.3	NP_651274.1	atlastin, isoform A
NM_170157.2	NP_733020.1	atlastin, isoform B
NM_001300577.1	NP_001287506.1	atlastin, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	atl	NP_733020.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002047	XP_321004.2
Caenorhabditis elegans (roundworm)	Y54G2A.2	NP_001023492.1
	LOC710647	XP_002808081.1
Canis lupus familiaris (dog)	ATL2	XP_003639636.1
Mus musculus (house mouse)	Atl2	NP_062691.3
Rattus norvegicus (Norway rat)	Atl2	NP_001094141.1
Pan troglodytes (chimpanzee)	ATL2	XP_001168056.1
Gallus gallus (chicken)	ATL2	XP_003640977.2
Xenopus tropicalis (tropical clawed frog)	atl2	XP_002940441.1
Bos taurus (cattle)	ATL2	NP_001229240.1
Danio rerio (zebrafish)	atl2	NP_001103492.1
Homo sapiens (human)	ATL2	NP_001129145.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this paper shows that atl is required in motor neurons to affect transmitter release
Title: The effects of ER morphology on synaptic structure and function in Drosophila melanogaster.

Data indicate that GTP hydrolysis is required for atlastin (ATL)-mediated vesicle tethering.
Title: Cis and trans interactions between atlastin molecules during membrane fusion.

purified and reconstituted human ATL1 exhibited no in vitro fusion activity. When the cytosolic segment of human ATL1 was connected to the transmembrane (TM) region and C-terminal tail (CT) of Drosophila ATL
Title: Comparison of human and Drosophila atlastin GTPases.

These data suggest that the C-terminal tail of Atlastin locally destabilizes bilayers to facilitate membrane fusion.
Title: The Atlastin C-terminal tail is an amphipathic helix that perturbs the bilayer structure during endoplasmic reticulum homotypic fusion.

Data indicate that expression of either the K320E or E328R atlastin GTPase (D-ATL) variant led to dominant-negative perturbations of the endoplasmic reticulum.
Title: Membrane tethering by the atlastin GTPase depends on GTP hydrolysis but not on forming the cross-over configuration.

The following atl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05976	NM_001300577.1 Latest version!	Drosophila melanogaster atlastin (atl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05976	NM_143017.3 Latest version!	Drosophila melanogaster atlastin (atl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05976	NM_170157.2 Latest version!	Drosophila melanogaster atlastin (atl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atl ( NM_143017.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651274.1 Drosophila melanogaster atlastin (atl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05976
Clone ID Related Accession (Same CDS sequence)	NM_143017.3 , NM_170157.2 , NM_001300577.1
Accession Version	NM_143017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	atlastin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143017.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGGAT CGGCAGTGCA GGTGATCAAC GCCTCCGAGG AGCACACATT TGTGCTCAAC 
GAGGATGCGC TGAGTGAGGT CCTAATGCGG GATGAGGTCA AGGATCGGTT CGTCTGCGTT 
GTCTCCGTGG CTGGAGCCTT CCGAAAGGGC AAGAGCTTCC TGCTGGACTT TTTTCTGCGC 
TATATGTATT CAAAGTATGT GCATCACGAT GCGACAGACT GGCTGGGAGG CGAATCAGAT 
CCGCTGGAGG GTTTCTCCTG GCGCGGCGGA TCTGAGCGCG ACACCACCGG CATTCTCATG 
TGGTCCGACA TATTCCTGCA CGACTATCCC AACGGCGACA AGATAGCCAT CATTCTGCTG 
GACACACAGG GCGCCTTCGA CAGCCAGAGC ACGGTGCGCG ATTGTGCCAC CGTTTTTGCG 
TTGAGCACAA TGCTGTCCTC GGTGCAGATA TACAACCTGT CACAGAACAT CCAGGAGGAC 
GACCTGCAGC ACCTGCAGCT CTTCACTGAG TATGGTCGCC TCGCGCTGGC CGACACCGGC 
AAAAAGCCGT TCCAGCGTCT GCAGTTCCTC GTCCGGGACT GGAGCTTTCC CTACGAGGCG 
GAATATGGTG CACTGGGCGG GGATAAGATT CTGAAACGAC GTCTGGAGGT GTCCGACAAA 
CAGCACCCAG AACTACAGTC CCTGCGTCGC CACATTTCGT CCTGTTTCAC GGAGGTGGCC 
TGCTTCCTGA TGCCCCATCC AGGTCTCAAT GTGGCCACCA ATCCTAAATT TGACGGTCGG 
CTGCAGGACA TCACGCCCGA GTTTAAGAGT AGCCTGCGCT CCCTGGTGCC CATGCTGCTG 
GCACCGGACA ACCTTGTCTA CAAGGAGATC AGCGGACAGC GGGTGCGGGC CCGCGATCTC 
ATCCAGTACT TCCAATCGTA CATGAACATC TACAAGGGCA ACGAGCTGCC CGAGCCGAAG 
AGCATGCTGG TGGCCACCGC AGAGGCTAAC CATTTGACTG CCGTGGCCGC CGCCAAGGAG 
CTGTACGGAC AGCTCATGGA GGAGGTGTGC GGTGGAACGC GGCCGTACTT AAGCACCGCC 
CATCTCCAGA CGGAGCACCT GCGGGTGAAG GACAAGGCAC TGTTCCAGTT CGCCGCCAAG 
CGCAAGATGG GTGGTGAGGA GTTCACCGAG AAATTCCGCA AGCAACTGGA AGATGATCTT 
GAGGAGGTCT TCACCAACTA CCAAGCACAC AACGAGAGCA AGAACATCTT TAAGGCAGCA 
CGGACACCGG CGGTGTACTT CGCCTGCGCC GTCATCATGT ACATCCTTAG CGGCATCTTT 
GGATTGGTGG GTCTCTATAC GTTCGCCAAC TTCTGCAACC TGGTTATGGG TGTGGCGCTT 
CTAACGCTGG CTCTGTGGGC CTACATTAGA TATAGCGGAG AGCTCAGCGA CTTTGGCGGC 
AAGTTGGATG ACTTTGCAAC GCTATTGTGG GAGAAATTCA TGCGACCCAT CTATCACGGC 
TGCATGGAGA AGGGCATCCA CCATGTTGCC ACTCATGCGA CCGAAATGGC TGTCGGCGGA 
GGCGCAGCCT CCTACCGCTC CCAGACCTCG GTGAATGCGT CCAATGGCAA GGTGAAGCGG 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651274.1
CDS	257..1882
Translation	MGGSAVQVINASEEHTFVLNEDALSEVLMRDEVKDRFVCVVSVAGAFRKGKSFLLDFFLRYMYSKYVHHDATDWLGGESDPLEGFSWRGGSERDTTGILMWSDIFLHDYPNGDKIAIILLDTQGAFDSQSTVRDCATVFALSTMLSSVQIYNLSQNIQEDDLQHLQLFTEYGRLALADTGKKPFQRLQFLVRDWSFPYEAEYGALGGDKILKRRLEVSDKQHPELQSLRRHISSCFTEVACFLMPHPGLNVATNPKFDGRLQDITPEFKSSLRSLVPMLLAPDNLVYKEISGQRVRARDLIQYFQSYMNIYKGNELPEPKSMLVATAEANHLTAVAAAKELYGQLMEEVCGGTRPYLSTAHLQTEHLRVKDKALFQFAAKRKMGGEEFTEKFRKQLEDDLEEVFTNYQAHNESKNIFKAARTPAVYFACAVIMYILSGIFGLVGLYTFANFCNLVMGVALLTLALWAYIRYSGELSDFGGKLDDFATLLWEKFMRPIYHGCMEKGIHHVATHATEMAVGGGAASYRSQTSVNASNGKVKRS

Target ORF information:

RefSeq Version	NM_143017.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster atlastin (atl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143017.3

ORF Insert Sequence:

ATGGGCGGAT CGGCAGTGCA GGTGATCAAC GCCTCCGAGG AGCACACATT TGTGCTCAAC 
GAGGATGCGC TGAGTGAGGT CCTAATGCGG GATGAGGTCA AGGATCGGTT CGTCTGCGTT 
GTCTCCGTGG CTGGAGCCTT CCGAAAGGGC AAGAGCTTCC TGCTGGACTT TTTTCTGCGC 
TATATGTATT CAAAGTATGT GCATCACGAT GCGACAGACT GGCTGGGAGG CGAATCAGAT 
CCGCTGGAGG GTTTCTCCTG GCGCGGCGGA TCTGAGCGCG ACACCACCGG CATTCTCATG 
TGGTCCGACA TATTCCTGCA CGACTATCCC AACGGCGACA AGATAGCCAT CATTCTGCTG 
GACACACAGG GCGCCTTCGA CAGCCAGAGC ACGGTGCGCG ATTGTGCCAC CGTTTTTGCG 
TTGAGCACAA TGCTGTCCTC GGTGCAGATA TACAACCTGT CACAGAACAT CCAGGAGGAC 
GACCTGCAGC ACCTGCAGCT CTTCACTGAG TATGGTCGCC TCGCGCTGGC CGACACCGGC 
AAAAAGCCGT TCCAGCGTCT GCAGTTCCTC GTCCGGGACT GGAGCTTTCC CTACGAGGCG 
GAATATGGTG CACTGGGCGG GGATAAGATT CTGAAACGAC GTCTGGAGGT GTCCGACAAA 
CAGCACCCAG AACTACAGTC CCTGCGTCGC CACATTTCGT CCTGTTTCAC GGAGGTGGCC 
TGCTTCCTGA TGCCCCATCC AGGTCTCAAT GTGGCCACCA ATCCTAAATT TGACGGTCGG 
CTGCAGGACA TCACGCCCGA GTTTAAGAGT AGCCTGCGCT CCCTGGTGCC CATGCTGCTG 
GCACCGGACA ACCTTGTCTA CAAGGAGATC AGCGGACAGC GGGTGCGGGC CCGCGATCTC 
ATCCAGTACT TCCAATCGTA CATGAACATC TACAAGGGCA ACGAGCTGCC CGAGCCGAAG 
AGCATGCTGG TGGCCACCGC AGAGGCTAAC CATTTGACTG CCGTGGCCGC CGCCAAGGAG 
CTGTACGGAC AGCTCATGGA GGAGGTGTGC GGTGGAACGC GGCCGTACTT AAGCACCGCC 
CATCTCCAGA CGGAGCACCT GCGGGTGAAG GACAAGGCAC TGTTCCAGTT CGCCGCCAAG 
CGCAAGATGG GTGGTGAGGA GTTCACCGAG AAATTCCGCA AGCAACTGGA AGATGATCTT 
GAGGAGGTCT TCACCAACTA CCAAGCACAC AACGAGAGCA AGAACATCTT TAAGGCAGCA 
CGGACACCGG CGGTGTACTT CGCCTGCGCC GTCATCATGT ACATCCTTAG CGGCATCTTT 
GGATTGGTGG GTCTCTATAC GTTCGCCAAC TTCTGCAACC TGGTTATGGG TGTGGCGCTT 
CTAACGCTGG CTCTGTGGGC CTACATTAGA TATAGCGGAG AGCTCAGCGA CTTTGGCGGC 
AAGTTGGATG ACTTTGCAAC GCTATTGTGG GAGAAATTCA TGCGACCCAT CTATCACGGC 
TGCATGGAGA AGGGCATCCA CCATGTTGCC ACTCATGCGA CCGAAATGGC TGTCGGCGGA 
GGCGCAGCCT CCTACCGCTC CCAGACCTCG GTGAATGCGT CCAATGGCAA GGTGAAGCGG 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atl ( NM_170157.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733020.1 Drosophila melanogaster atlastin (atl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05976
Clone ID Related Accession (Same CDS sequence)	NM_143017.3 , NM_170157.2 , NM_001300577.1
Accession Version	NM_170157.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	atlastin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170157.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGGAT CGGCAGTGCA GGTGATCAAC GCCTCCGAGG AGCACACATT TGTGCTCAAC 
GAGGATGCGC TGAGTGAGGT CCTAATGCGG GATGAGGTCA AGGATCGGTT CGTCTGCGTT 
GTCTCCGTGG CTGGAGCCTT CCGAAAGGGC AAGAGCTTCC TGCTGGACTT TTTTCTGCGC 
TATATGTATT CAAAGTATGT GCATCACGAT GCGACAGACT GGCTGGGAGG CGAATCAGAT 
CCGCTGGAGG GTTTCTCCTG GCGCGGCGGA TCTGAGCGCG ACACCACCGG CATTCTCATG 
TGGTCCGACA TATTCCTGCA CGACTATCCC AACGGCGACA AGATAGCCAT CATTCTGCTG 
GACACACAGG GCGCCTTCGA CAGCCAGAGC ACGGTGCGCG ATTGTGCCAC CGTTTTTGCG 
TTGAGCACAA TGCTGTCCTC GGTGCAGATA TACAACCTGT CACAGAACAT CCAGGAGGAC 
GACCTGCAGC ACCTGCAGCT CTTCACTGAG TATGGTCGCC TCGCGCTGGC CGACACCGGC 
AAAAAGCCGT TCCAGCGTCT GCAGTTCCTC GTCCGGGACT GGAGCTTTCC CTACGAGGCG 
GAATATGGTG CACTGGGCGG GGATAAGATT CTGAAACGAC GTCTGGAGGT GTCCGACAAA 
CAGCACCCAG AACTACAGTC CCTGCGTCGC CACATTTCGT CCTGTTTCAC GGAGGTGGCC 
TGCTTCCTGA TGCCCCATCC AGGTCTCAAT GTGGCCACCA ATCCTAAATT TGACGGTCGG 
CTGCAGGACA TCACGCCCGA GTTTAAGAGT AGCCTGCGCT CCCTGGTGCC CATGCTGCTG 
GCACCGGACA ACCTTGTCTA CAAGGAGATC AGCGGACAGC GGGTGCGGGC CCGCGATCTC 
ATCCAGTACT TCCAATCGTA CATGAACATC TACAAGGGCA ACGAGCTGCC CGAGCCGAAG 
AGCATGCTGG TGGCCACCGC AGAGGCTAAC CATTTGACTG CCGTGGCCGC CGCCAAGGAG 
CTGTACGGAC AGCTCATGGA GGAGGTGTGC GGTGGAACGC GGCCGTACTT AAGCACCGCC 
CATCTCCAGA CGGAGCACCT GCGGGTGAAG GACAAGGCAC TGTTCCAGTT CGCCGCCAAG 
CGCAAGATGG GTGGTGAGGA GTTCACCGAG AAATTCCGCA AGCAACTGGA AGATGATCTT 
GAGGAGGTCT TCACCAACTA CCAAGCACAC AACGAGAGCA AGAACATCTT TAAGGCAGCA 
CGGACACCGG CGGTGTACTT CGCCTGCGCC GTCATCATGT ACATCCTTAG CGGCATCTTT 
GGATTGGTGG GTCTCTATAC GTTCGCCAAC TTCTGCAACC TGGTTATGGG TGTGGCGCTT 
CTAACGCTGG CTCTGTGGGC CTACATTAGA TATAGCGGAG AGCTCAGCGA CTTTGGCGGC 
AAGTTGGATG ACTTTGCAAC GCTATTGTGG GAGAAATTCA TGCGACCCAT CTATCACGGC 
TGCATGGAGA AGGGCATCCA CCATGTTGCC ACTCATGCGA CCGAAATGGC TGTCGGCGGA 
GGCGCAGCCT CCTACCGCTC CCAGACCTCG GTGAATGCGT CCAATGGCAA GGTGAAGCGG 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733020.1
CDS	575..2200
Translation	MGGSAVQVINASEEHTFVLNEDALSEVLMRDEVKDRFVCVVSVAGAFRKGKSFLLDFFLRYMYSKYVHHDATDWLGGESDPLEGFSWRGGSERDTTGILMWSDIFLHDYPNGDKIAIILLDTQGAFDSQSTVRDCATVFALSTMLSSVQIYNLSQNIQEDDLQHLQLFTEYGRLALADTGKKPFQRLQFLVRDWSFPYEAEYGALGGDKILKRRLEVSDKQHPELQSLRRHISSCFTEVACFLMPHPGLNVATNPKFDGRLQDITPEFKSSLRSLVPMLLAPDNLVYKEISGQRVRARDLIQYFQSYMNIYKGNELPEPKSMLVATAEANHLTAVAAAKELYGQLMEEVCGGTRPYLSTAHLQTEHLRVKDKALFQFAAKRKMGGEEFTEKFRKQLEDDLEEVFTNYQAHNESKNIFKAARTPAVYFACAVIMYILSGIFGLVGLYTFANFCNLVMGVALLTLALWAYIRYSGELSDFGGKLDDFATLLWEKFMRPIYHGCMEKGIHHVATHATEMAVGGGAASYRSQTSVNASNGKVKRS

Target ORF information:

RefSeq Version	NM_170157.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster atlastin (atl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170157.2

ORF Insert Sequence:

ATGGGCGGAT CGGCAGTGCA GGTGATCAAC GCCTCCGAGG AGCACACATT TGTGCTCAAC 
GAGGATGCGC TGAGTGAGGT CCTAATGCGG GATGAGGTCA AGGATCGGTT CGTCTGCGTT 
GTCTCCGTGG CTGGAGCCTT CCGAAAGGGC AAGAGCTTCC TGCTGGACTT TTTTCTGCGC 
TATATGTATT CAAAGTATGT GCATCACGAT GCGACAGACT GGCTGGGAGG CGAATCAGAT 
CCGCTGGAGG GTTTCTCCTG GCGCGGCGGA TCTGAGCGCG ACACCACCGG CATTCTCATG 
TGGTCCGACA TATTCCTGCA CGACTATCCC AACGGCGACA AGATAGCCAT CATTCTGCTG 
GACACACAGG GCGCCTTCGA CAGCCAGAGC ACGGTGCGCG ATTGTGCCAC CGTTTTTGCG 
TTGAGCACAA TGCTGTCCTC GGTGCAGATA TACAACCTGT CACAGAACAT CCAGGAGGAC 
GACCTGCAGC ACCTGCAGCT CTTCACTGAG TATGGTCGCC TCGCGCTGGC CGACACCGGC 
AAAAAGCCGT TCCAGCGTCT GCAGTTCCTC GTCCGGGACT GGAGCTTTCC CTACGAGGCG 
GAATATGGTG CACTGGGCGG GGATAAGATT CTGAAACGAC GTCTGGAGGT GTCCGACAAA 
CAGCACCCAG AACTACAGTC CCTGCGTCGC CACATTTCGT CCTGTTTCAC GGAGGTGGCC 
TGCTTCCTGA TGCCCCATCC AGGTCTCAAT GTGGCCACCA ATCCTAAATT TGACGGTCGG 
CTGCAGGACA TCACGCCCGA GTTTAAGAGT AGCCTGCGCT CCCTGGTGCC CATGCTGCTG 
GCACCGGACA ACCTTGTCTA CAAGGAGATC AGCGGACAGC GGGTGCGGGC CCGCGATCTC 
ATCCAGTACT TCCAATCGTA CATGAACATC TACAAGGGCA ACGAGCTGCC CGAGCCGAAG 
AGCATGCTGG TGGCCACCGC AGAGGCTAAC CATTTGACTG CCGTGGCCGC CGCCAAGGAG 
CTGTACGGAC AGCTCATGGA GGAGGTGTGC GGTGGAACGC GGCCGTACTT AAGCACCGCC 
CATCTCCAGA CGGAGCACCT GCGGGTGAAG GACAAGGCAC TGTTCCAGTT CGCCGCCAAG 
CGCAAGATGG GTGGTGAGGA GTTCACCGAG AAATTCCGCA AGCAACTGGA AGATGATCTT 
GAGGAGGTCT TCACCAACTA CCAAGCACAC AACGAGAGCA AGAACATCTT TAAGGCAGCA 
CGGACACCGG CGGTGTACTT CGCCTGCGCC GTCATCATGT ACATCCTTAG CGGCATCTTT 
GGATTGGTGG GTCTCTATAC GTTCGCCAAC TTCTGCAACC TGGTTATGGG TGTGGCGCTT 
CTAACGCTGG CTCTGTGGGC CTACATTAGA TATAGCGGAG AGCTCAGCGA CTTTGGCGGC 
AAGTTGGATG ACTTTGCAAC GCTATTGTGG GAGAAATTCA TGCGACCCAT CTATCACGGC 
TGCATGGAGA AGGGCATCCA CCATGTTGCC ACTCATGCGA CCGAAATGGC TGTCGGCGGA 
GGCGCAGCCT CCTACCGCTC CCAGACCTCG GTGAATGCGT CCAATGGCAA GGTGAAGCGG 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atl ( NM_001300577.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287506.1 Drosophila melanogaster atlastin (atl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05976
Clone ID Related Accession (Same CDS sequence)	NM_143017.3 , NM_170157.2 , NM_001300577.1
Accession Version	NM_001300577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	atlastin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGGAT CGGCAGTGCA GGTGATCAAC GCCTCCGAGG AGCACACATT TGTGCTCAAC 
GAGGATGCGC TGAGTGAGGT CCTAATGCGG GATGAGGTCA AGGATCGGTT CGTCTGCGTT 
GTCTCCGTGG CTGGAGCCTT CCGAAAGGGC AAGAGCTTCC TGCTGGACTT TTTTCTGCGC 
TATATGTATT CAAAGTATGT GCATCACGAT GCGACAGACT GGCTGGGAGG CGAATCAGAT 
CCGCTGGAGG GTTTCTCCTG GCGCGGCGGA TCTGAGCGCG ACACCACCGG CATTCTCATG 
TGGTCCGACA TATTCCTGCA CGACTATCCC AACGGCGACA AGATAGCCAT CATTCTGCTG 
GACACACAGG GCGCCTTCGA CAGCCAGAGC ACGGTGCGCG ATTGTGCCAC CGTTTTTGCG 
TTGAGCACAA TGCTGTCCTC GGTGCAGATA TACAACCTGT CACAGAACAT CCAGGAGGAC 
GACCTGCAGC ACCTGCAGCT CTTCACTGAG TATGGTCGCC TCGCGCTGGC CGACACCGGC 
AAAAAGCCGT TCCAGCGTCT GCAGTTCCTC GTCCGGGACT GGAGCTTTCC CTACGAGGCG 
GAATATGGTG CACTGGGCGG GGATAAGATT CTGAAACGAC GTCTGGAGGT GTCCGACAAA 
CAGCACCCAG AACTACAGTC CCTGCGTCGC CACATTTCGT CCTGTTTCAC GGAGGTGGCC 
TGCTTCCTGA TGCCCCATCC AGGTCTCAAT GTGGCCACCA ATCCTAAATT TGACGGTCGG 
CTGCAGGACA TCACGCCCGA GTTTAAGAGT AGCCTGCGCT CCCTGGTGCC CATGCTGCTG 
GCACCGGACA ACCTTGTCTA CAAGGAGATC AGCGGACAGC GGGTGCGGGC CCGCGATCTC 
ATCCAGTACT TCCAATCGTA CATGAACATC TACAAGGGCA ACGAGCTGCC CGAGCCGAAG 
AGCATGCTGG TGGCCACCGC AGAGGCTAAC CATTTGACTG CCGTGGCCGC CGCCAAGGAG 
CTGTACGGAC AGCTCATGGA GGAGGTGTGC GGTGGAACGC GGCCGTACTT AAGCACCGCC 
CATCTCCAGA CGGAGCACCT GCGGGTGAAG GACAAGGCAC TGTTCCAGTT CGCCGCCAAG 
CGCAAGATGG GTGGTGAGGA GTTCACCGAG AAATTCCGCA AGCAACTGGA AGATGATCTT 
GAGGAGGTCT TCACCAACTA CCAAGCACAC AACGAGAGCA AGAACATCTT TAAGGCAGCA 
CGGACACCGG CGGTGTACTT CGCCTGCGCC GTCATCATGT ACATCCTTAG CGGCATCTTT 
GGATTGGTGG GTCTCTATAC GTTCGCCAAC TTCTGCAACC TGGTTATGGG TGTGGCGCTT 
CTAACGCTGG CTCTGTGGGC CTACATTAGA TATAGCGGAG AGCTCAGCGA CTTTGGCGGC 
AAGTTGGATG ACTTTGCAAC GCTATTGTGG GAGAAATTCA TGCGACCCAT CTATCACGGC 
TGCATGGAGA AGGGCATCCA CCATGTTGCC ACTCATGCGA CCGAAATGGC TGTCGGCGGA 
GGCGCAGCCT CCTACCGCTC CCAGACCTCG GTGAATGCGT CCAATGGCAA GGTGAAGCGG 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287506.1
CDS	275..1900
Translation	MGGSAVQVINASEEHTFVLNEDALSEVLMRDEVKDRFVCVVSVAGAFRKGKSFLLDFFLRYMYSKYVHHDATDWLGGESDPLEGFSWRGGSERDTTGILMWSDIFLHDYPNGDKIAIILLDTQGAFDSQSTVRDCATVFALSTMLSSVQIYNLSQNIQEDDLQHLQLFTEYGRLALADTGKKPFQRLQFLVRDWSFPYEAEYGALGGDKILKRRLEVSDKQHPELQSLRRHISSCFTEVACFLMPHPGLNVATNPKFDGRLQDITPEFKSSLRSLVPMLLAPDNLVYKEISGQRVRARDLIQYFQSYMNIYKGNELPEPKSMLVATAEANHLTAVAAAKELYGQLMEEVCGGTRPYLSTAHLQTEHLRVKDKALFQFAAKRKMGGEEFTEKFRKQLEDDLEEVFTNYQAHNESKNIFKAARTPAVYFACAVIMYILSGIFGLVGLYTFANFCNLVMGVALLTLALWAYIRYSGELSDFGGKLDDFATLLWEKFMRPIYHGCMEKGIHHVATHATEMAVGGGAASYRSQTSVNASNGKVKRS

Target ORF information:

RefSeq Version	NM_001300577.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster atlastin (atl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300577.1

ORF Insert Sequence:

ATGGGCGGAT CGGCAGTGCA GGTGATCAAC GCCTCCGAGG AGCACACATT TGTGCTCAAC 
GAGGATGCGC TGAGTGAGGT CCTAATGCGG GATGAGGTCA AGGATCGGTT CGTCTGCGTT 
GTCTCCGTGG CTGGAGCCTT CCGAAAGGGC AAGAGCTTCC TGCTGGACTT TTTTCTGCGC 
TATATGTATT CAAAGTATGT GCATCACGAT GCGACAGACT GGCTGGGAGG CGAATCAGAT 
CCGCTGGAGG GTTTCTCCTG GCGCGGCGGA TCTGAGCGCG ACACCACCGG CATTCTCATG 
TGGTCCGACA TATTCCTGCA CGACTATCCC AACGGCGACA AGATAGCCAT CATTCTGCTG 
GACACACAGG GCGCCTTCGA CAGCCAGAGC ACGGTGCGCG ATTGTGCCAC CGTTTTTGCG 
TTGAGCACAA TGCTGTCCTC GGTGCAGATA TACAACCTGT CACAGAACAT CCAGGAGGAC 
GACCTGCAGC ACCTGCAGCT CTTCACTGAG TATGGTCGCC TCGCGCTGGC CGACACCGGC 
AAAAAGCCGT TCCAGCGTCT GCAGTTCCTC GTCCGGGACT GGAGCTTTCC CTACGAGGCG 
GAATATGGTG CACTGGGCGG GGATAAGATT CTGAAACGAC GTCTGGAGGT GTCCGACAAA 
CAGCACCCAG AACTACAGTC CCTGCGTCGC CACATTTCGT CCTGTTTCAC GGAGGTGGCC 
TGCTTCCTGA TGCCCCATCC AGGTCTCAAT GTGGCCACCA ATCCTAAATT TGACGGTCGG 
CTGCAGGACA TCACGCCCGA GTTTAAGAGT AGCCTGCGCT CCCTGGTGCC CATGCTGCTG 
GCACCGGACA ACCTTGTCTA CAAGGAGATC AGCGGACAGC GGGTGCGGGC CCGCGATCTC 
ATCCAGTACT TCCAATCGTA CATGAACATC TACAAGGGCA ACGAGCTGCC CGAGCCGAAG 
AGCATGCTGG TGGCCACCGC AGAGGCTAAC CATTTGACTG CCGTGGCCGC CGCCAAGGAG 
CTGTACGGAC AGCTCATGGA GGAGGTGTGC GGTGGAACGC GGCCGTACTT AAGCACCGCC 
CATCTCCAGA CGGAGCACCT GCGGGTGAAG GACAAGGCAC TGTTCCAGTT CGCCGCCAAG 
CGCAAGATGG GTGGTGAGGA GTTCACCGAG AAATTCCGCA AGCAACTGGA AGATGATCTT 
GAGGAGGTCT TCACCAACTA CCAAGCACAC AACGAGAGCA AGAACATCTT TAAGGCAGCA 
CGGACACCGG CGGTGTACTT CGCCTGCGCC GTCATCATGT ACATCCTTAG CGGCATCTTT 
GGATTGGTGG GTCTCTATAC GTTCGCCAAC TTCTGCAACC TGGTTATGGG TGTGGCGCTT 
CTAACGCTGG CTCTGTGGGC CTACATTAGA TATAGCGGAG AGCTCAGCGA CTTTGGCGGC 
AAGTTGGATG ACTTTGCAAC GCTATTGTGG GAGAAATTCA TGCGACCCAT CTATCACGGC 
TGCATGGAGA AGGGCATCCA CCATGTTGCC ACTCATGCGA CCGAAATGGC TGTCGGCGGA 
GGCGCAGCCT CCTACCGCTC CCAGACCTCG GTGAATGCGT CCAATGGCAA GGTGAAGCGG 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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atl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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