nAChRalpha2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nAChRalpha2
Entrez Gene ID	42919
Full Name	nicotinic Acetylcholine Receptor alpha2
Synonyms	Acr96Ab,AcrE,CG6844,CG6844-PA,D[a2],Da2,Dalpha,Dalpha2,Dalpha2/SAD,Dmalpha2,Dmel\CG6844,SAD,alpha2,alpha[[2]],da2,nAChR,nAChR Dalpha2,nAChR-alpha96Ab,nAChRa2,nAChRalpha-96A,nAcRalpha-96Ab,nAcRs,nAchRalpha-96Ab,nAcr96Ab,sad
General protein information	Preferred Names nicotinic Acetylcholine Receptor alpha2 Names CG6844 gene product from transcript CG6844-RA CG6844-PA CG6844-PB Dalpha2 Dalpha2 nicotimic acetylcholine receptor SAD drosophila alpha-like subunit 2 nAChRalpha2-PA nAChRalpha2-PB nicotinic acetylcholine receptor alpha 96Ab nicotinic acetylcholine receptor alpha subunit gene at 96AB nicotinic acetylcholine receptor alpha-2 nicotinic acetylcholine receptor alpha2 second alpha-like subunit of drosophila
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96A3-96A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_079758.3	NP_524482.1	nicotinic acetylcholine receptor alpha2, isoform A
NM_170146.2	NP_733001.1	nicotinic acetylcholine receptor alpha2, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHRNA2	XP_005635946.1
Mus musculus (house mouse)	Chrna2	NP_659052.1
Rattus norvegicus (Norway rat)	Chrna2	NP_596911.1
Pan troglodytes (chimpanzee)	CHRNA2	NP_001029107.1
	CHRNA2	XP_001109335.1
Xenopus tropicalis (tropical clawed frog)	chrna2	XP_002941292.2
Homo sapiens (human)	CHRNA2	NP_000733.2
Gallus gallus (chicken)	CHRNA2	NP_990146.1
Bos taurus (cattle)	CHRNA2	NP_001179639.1
Drosophila melanogaster (fruit fly)	nAcRalpha-96Ab	NP_733001.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002972	XP_311921.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nAChRalpha2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nAChRalpha2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05959	NM_170146.2 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha2, transcript variant B (nAChRalpha2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05959	NM_079758.3 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha2, transcript variant A (nAChRalpha2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nAChRalpha2 ( NM_079758.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524482.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha2, transcript variant A (nAChRalpha2), mRNA.

Price & Availability↑

CloneID	OFa05959
Clone ID Related Accession (Same CDS sequence)	NM_079758.3 , NM_170146.2
Accession Version	NM_079758.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079758.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCCTG GCTGCTGCAC CACACGACCC CGCCCCATCG CCCTGCTCGC TCACATCTGG 
CGCCACTGCA AGCCACTCTG CCTGCTCCTG GTGCTCCTGC TCCTCTGCGA AACCGTTCAG 
GCGAATCCCG ATGCCAAGCG ACTCTATGAC GATCTGCTAA GCAACTACAA TCGCCTCATC 
CGCCCTGTGA GCAATAATAC GGACACGGTG TTGGTCAAAT TGGGCCTACG GCTCTCCCAA 
CTCATCGATT TGAATCTCAA AGATCAAATT CTAACCACCA ACGTGTGGCT GGAGCACGAA 
TGGCAGGATC ATAAATTCAA GTGGGATCCC TCGGAGTATG GCGGCGTCAC AGAGCTTTAT 
GTGCCATCCG AGCACATCTG GCTGCCCGAC ATCGTGCTCT ACAACAATGC CGATGGCGAG 
TACGTGGTCA CCACCATGAC GAAGGCCATC CTCCACTATA CCGGCAAAGT GGTCTGGACT 
CCGCCGGCCA TCTTCAAGTC CAGCTGTGAG ATTGATGTGC GCTACTTTCC CTTCGATCAG 
CAGACCTGCT TCATGAAGTT CGGCTCGTGG ACCTACGACG GTGATCAGAT CGATTTGAAG 
CACATCAGCC AGAAAAACGA CAAGGACAAC AAGGTGGAGA TAGGCATCGA CCTGCGTGAG 
TACTATCCCA GTGTGGAGTG GGACATTCTC GGCGTTCCGG CTGAGCGGCA CGAGAAGTAC 
TATCCCTGCT GTGCCGAACC GTATCCGGAT ATCTTCTTCA ACATCACCCT GAGGCGAAAG 
ACTCTCTTCT ACACGGTTAA CCTGATTATT CCATGTGTGG GCATCTCGTA TCTTTCGGTG 
CTGGTCTTTT ACCTGCCCGC CGATTCTGGC GAGAAGATTG CTCTGTGCAT CAGCATCCTG 
CTGTCGCAAA CCATGTTCTT CCTGCTCATA TCGGAGATTA TACCATCGAC TTCACTGGCA 
TTGCCGCTAC TGGGAAAATA TCTACTGTTC ACCATGTTGC TGGTTGGCCT GAGTGTTGTC 
ATCACGATTA TCATACTAAA CATACACTAC CGGAAGCCGA GTACGCACAA GATGCGACCC 
TGGATTAGGT CCTTCTTCAT CAAGAGATTG CCGAAACTCC TGCTGATGCG TGTGCCCAAG 
GACCTGCTGC GTGACCTGGC GGCCAACAAG ATCAACTACG GCCTCAAGTT CAGCAAGACC 
AAGTTCGGAC AGGCGCTGAT GGACGAGATG CAAATGAACT CGGGCGGCTC CAGTCCAGAC 
TCCCTGCGGC GGATGCAAGG TCGTGTGGGT GCTGGTGGGT GCAATGGCAT GCACGTGACC 
ACGGCCACAA ACAGATTCAG CGGCTTGGTG GGGGCTTTGG GCGGCGGACT GAGCACCCTG 
AGCGGCTACA ACGGACTGCC ATCGGTGCTA TCCGGATTGG ACGACTCTTT GAGCGATGTG 
GCCGCACGCA AAAAGTATCC TTTCGAGCTG GAAAAGGCCA TCCACAACGT CATGTTCATA 
CAGCACCACA TGCAGCGGCA GGACGAGTTC AATGCGGAAG ATCAGGACTG GGGCTTTGTG 
GCCATGGTCA TGGATCGCCT ATTCCTCTGG CTCTTTATGA TCGCATCCTT GGTGGGCACA 
TTTGTGATCC TAGGCGAGGC TCCGTCGCTA TATGACGATA CCAAGGCCAT TGATGTTCAG 
CTATCCGATG TTGCCAAGCA AATCTACAAT CTAACCGAGA AGAAGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524482.1
CDS	522..2252
Translation	MAPGCCTTRPRPIALLAHIWRHCKPLCLLLVLLLLCETVQANPDAKRLYDDLLSNYNRLIRPVSNNTDTVLVKLGLRLSQLIDLNLKDQILTTNVWLEHEWQDHKFKWDPSEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTMTKAILHYTGKVVWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHISQKNDKDNKVEIGIDLREYYPSVEWDILGVPAERHEKYYPCCAEPYPDIFFNITLRRKTLFYTVNLIIPCVGISYLSVLVFYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMLLVGLSVVITIIILNIHYRKPSTHKMRPWIRSFFIKRLPKLLLMRVPKDLLRDLAANKINYGLKFSKTKFGQALMDEMQMNSGGSSPDSLRRMQGRVGAGGCNGMHVTTATNRFSGLVGALGGGLSTLSGYNGLPSVLSGLDDSLSDVAARKKYPFELEKAIHNVMFIQHHMQRQDEFNAEDQDWGFVAMVMDRLFLWLFMIASLVGTFVILGEAPSLYDDTKAIDVQLSDVAKQIYNLTEKKN

Target ORF information:

RefSeq Version	NM_079758.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha2, transcript variant A (nAChRalpha2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079758.3

ORF Insert Sequence:

ATGGCTCCTG GCTGCTGCAC CACACGACCC CGCCCCATCG CCCTGCTCGC TCACATCTGG 
CGCCACTGCA AGCCACTCTG CCTGCTCCTG GTGCTCCTGC TCCTCTGCGA AACCGTTCAG 
GCGAATCCCG ATGCCAAGCG ACTCTATGAC GATCTGCTAA GCAACTACAA TCGCCTCATC 
CGCCCTGTGA GCAATAATAC GGACACGGTG TTGGTCAAAT TGGGCCTACG GCTCTCCCAA 
CTCATCGATT TGAATCTCAA AGATCAAATT CTAACCACCA ACGTGTGGCT GGAGCACGAA 
TGGCAGGATC ATAAATTCAA GTGGGATCCC TCGGAGTATG GCGGCGTCAC AGAGCTTTAT 
GTGCCATCCG AGCACATCTG GCTGCCCGAC ATCGTGCTCT ACAACAATGC CGATGGCGAG 
TACGTGGTCA CCACCATGAC GAAGGCCATC CTCCACTATA CCGGCAAAGT GGTCTGGACT 
CCGCCGGCCA TCTTCAAGTC CAGCTGTGAG ATTGATGTGC GCTACTTTCC CTTCGATCAG 
CAGACCTGCT TCATGAAGTT CGGCTCGTGG ACCTACGACG GTGATCAGAT CGATTTGAAG 
CACATCAGCC AGAAAAACGA CAAGGACAAC AAGGTGGAGA TAGGCATCGA CCTGCGTGAG 
TACTATCCCA GTGTGGAGTG GGACATTCTC GGCGTTCCGG CTGAGCGGCA CGAGAAGTAC 
TATCCCTGCT GTGCCGAACC GTATCCGGAT ATCTTCTTCA ACATCACCCT GAGGCGAAAG 
ACTCTCTTCT ACACGGTTAA CCTGATTATT CCATGTGTGG GCATCTCGTA TCTTTCGGTG 
CTGGTCTTTT ACCTGCCCGC CGATTCTGGC GAGAAGATTG CTCTGTGCAT CAGCATCCTG 
CTGTCGCAAA CCATGTTCTT CCTGCTCATA TCGGAGATTA TACCATCGAC TTCACTGGCA 
TTGCCGCTAC TGGGAAAATA TCTACTGTTC ACCATGTTGC TGGTTGGCCT GAGTGTTGTC 
ATCACGATTA TCATACTAAA CATACACTAC CGGAAGCCGA GTACGCACAA GATGCGACCC 
TGGATTAGGT CCTTCTTCAT CAAGAGATTG CCGAAACTCC TGCTGATGCG TGTGCCCAAG 
GACCTGCTGC GTGACCTGGC GGCCAACAAG ATCAACTACG GCCTCAAGTT CAGCAAGACC 
AAGTTCGGAC AGGCGCTGAT GGACGAGATG CAAATGAACT CGGGCGGCTC CAGTCCAGAC 
TCCCTGCGGC GGATGCAAGG TCGTGTGGGT GCTGGTGGGT GCAATGGCAT GCACGTGACC 
ACGGCCACAA ACAGATTCAG CGGCTTGGTG GGGGCTTTGG GCGGCGGACT GAGCACCCTG 
AGCGGCTACA ACGGACTGCC ATCGGTGCTA TCCGGATTGG ACGACTCTTT GAGCGATGTG 
GCCGCACGCA AAAAGTATCC TTTCGAGCTG GAAAAGGCCA TCCACAACGT CATGTTCATA 
CAGCACCACA TGCAGCGGCA GGACGAGTTC AATGCGGAAG ATCAGGACTG GGGCTTTGTG 
GCCATGGTCA TGGATCGCCT ATTCCTCTGG CTCTTTATGA TCGCATCCTT GGTGGGCACA 
TTTGTGATCC TAGGCGAGGC TCCGTCGCTA TATGACGATA CCAAGGCCAT TGATGTTCAG 
CTATCCGATG TTGCCAAGCA AATCTACAAT CTAACCGAGA AGAAGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha2 ( NM_170146.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733001.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha2, transcript variant B (nAChRalpha2), mRNA.

Price & Availability↑

CloneID	OFa05959
Clone ID Related Accession (Same CDS sequence)	NM_079758.3 , NM_170146.2
Accession Version	NM_170146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170146.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCCTG GCTGCTGCAC CACACGACCC CGCCCCATCG CCCTGCTCGC TCACATCTGG 
CGCCACTGCA AGCCACTCTG CCTGCTCCTG GTGCTCCTGC TCCTCTGCGA AACCGTTCAG 
GCGAATCCCG ATGCCAAGCG ACTCTATGAC GATCTGCTAA GCAACTACAA TCGCCTCATC 
CGCCCTGTGA GCAATAATAC GGACACGGTG TTGGTCAAAT TGGGCCTACG GCTCTCCCAA 
CTCATCGATT TGAATCTCAA AGATCAAATT CTAACCACCA ACGTGTGGCT GGAGCACGAA 
TGGCAGGATC ATAAATTCAA GTGGGATCCC TCGGAGTATG GCGGCGTCAC AGAGCTTTAT 
GTGCCATCCG AGCACATCTG GCTGCCCGAC ATCGTGCTCT ACAACAATGC CGATGGCGAG 
TACGTGGTCA CCACCATGAC GAAGGCCATC CTCCACTATA CCGGCAAAGT GGTCTGGACT 
CCGCCGGCCA TCTTCAAGTC CAGCTGTGAG ATTGATGTGC GCTACTTTCC CTTCGATCAG 
CAGACCTGCT TCATGAAGTT CGGCTCGTGG ACCTACGACG GTGATCAGAT CGATTTGAAG 
CACATCAGCC AGAAAAACGA CAAGGACAAC AAGGTGGAGA TAGGCATCGA CCTGCGTGAG 
TACTATCCCA GTGTGGAGTG GGACATTCTC GGCGTTCCGG CTGAGCGGCA CGAGAAGTAC 
TATCCCTGCT GTGCCGAACC GTATCCGGAT ATCTTCTTCA ACATCACCCT GAGGCGAAAG 
ACTCTCTTCT ACACGGTTAA CCTGATTATT CCATGTGTGG GCATCTCGTA TCTTTCGGTG 
CTGGTCTTTT ACCTGCCCGC CGATTCTGGC GAGAAGATTG CTCTGTGCAT CAGCATCCTG 
CTGTCGCAAA CCATGTTCTT CCTGCTCATA TCGGAGATTA TACCATCGAC TTCACTGGCA 
TTGCCGCTAC TGGGAAAATA TCTACTGTTC ACCATGTTGC TGGTTGGCCT GAGTGTTGTC 
ATCACGATTA TCATACTAAA CATACACTAC CGGAAGCCGA GTACGCACAA GATGCGACCC 
TGGATTAGGT CCTTCTTCAT CAAGAGATTG CCGAAACTCC TGCTGATGCG TGTGCCCAAG 
GACCTGCTGC GTGACCTGGC GGCCAACAAG ATCAACTACG GCCTCAAGTT CAGCAAGACC 
AAGTTCGGAC AGGCGCTGAT GGACGAGATG CAAATGAACT CGGGCGGCTC CAGTCCAGAC 
TCCCTGCGGC GGATGCAAGG TCGTGTGGGT GCTGGTGGGT GCAATGGCAT GCACGTGACC 
ACGGCCACAA ACAGATTCAG CGGCTTGGTG GGGGCTTTGG GCGGCGGACT GAGCACCCTG 
AGCGGCTACA ACGGACTGCC ATCGGTGCTA TCCGGATTGG ACGACTCTTT GAGCGATGTG 
GCCGCACGCA AAAAGTATCC TTTCGAGCTG GAAAAGGCCA TCCACAACGT CATGTTCATA 
CAGCACCACA TGCAGCGGCA GGACGAGTTC AATGCGGAAG ATCAGGACTG GGGCTTTGTG 
GCCATGGTCA TGGATCGCCT ATTCCTCTGG CTCTTTATGA TCGCATCCTT GGTGGGCACA 
TTTGTGATCC TAGGCGAGGC TCCGTCGCTA TATGACGATA CCAAGGCCAT TGATGTTCAG 
CTATCCGATG TTGCCAAGCA AATCTACAAT CTAACCGAGA AGAAGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733001.1
CDS	454..2184
Translation	MAPGCCTTRPRPIALLAHIWRHCKPLCLLLVLLLLCETVQANPDAKRLYDDLLSNYNRLIRPVSNNTDTVLVKLGLRLSQLIDLNLKDQILTTNVWLEHEWQDHKFKWDPSEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTMTKAILHYTGKVVWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHISQKNDKDNKVEIGIDLREYYPSVEWDILGVPAERHEKYYPCCAEPYPDIFFNITLRRKTLFYTVNLIIPCVGISYLSVLVFYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMLLVGLSVVITIIILNIHYRKPSTHKMRPWIRSFFIKRLPKLLLMRVPKDLLRDLAANKINYGLKFSKTKFGQALMDEMQMNSGGSSPDSLRRMQGRVGAGGCNGMHVTTATNRFSGLVGALGGGLSTLSGYNGLPSVLSGLDDSLSDVAARKKYPFELEKAIHNVMFIQHHMQRQDEFNAEDQDWGFVAMVMDRLFLWLFMIASLVGTFVILGEAPSLYDDTKAIDVQLSDVAKQIYNLTEKKN

Target ORF information:

RefSeq Version	NM_170146.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha2, transcript variant B (nAChRalpha2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170146.2

ORF Insert Sequence:

ATGGCTCCTG GCTGCTGCAC CACACGACCC CGCCCCATCG CCCTGCTCGC TCACATCTGG 
CGCCACTGCA AGCCACTCTG CCTGCTCCTG GTGCTCCTGC TCCTCTGCGA AACCGTTCAG 
GCGAATCCCG ATGCCAAGCG ACTCTATGAC GATCTGCTAA GCAACTACAA TCGCCTCATC 
CGCCCTGTGA GCAATAATAC GGACACGGTG TTGGTCAAAT TGGGCCTACG GCTCTCCCAA 
CTCATCGATT TGAATCTCAA AGATCAAATT CTAACCACCA ACGTGTGGCT GGAGCACGAA 
TGGCAGGATC ATAAATTCAA GTGGGATCCC TCGGAGTATG GCGGCGTCAC AGAGCTTTAT 
GTGCCATCCG AGCACATCTG GCTGCCCGAC ATCGTGCTCT ACAACAATGC CGATGGCGAG 
TACGTGGTCA CCACCATGAC GAAGGCCATC CTCCACTATA CCGGCAAAGT GGTCTGGACT 
CCGCCGGCCA TCTTCAAGTC CAGCTGTGAG ATTGATGTGC GCTACTTTCC CTTCGATCAG 
CAGACCTGCT TCATGAAGTT CGGCTCGTGG ACCTACGACG GTGATCAGAT CGATTTGAAG 
CACATCAGCC AGAAAAACGA CAAGGACAAC AAGGTGGAGA TAGGCATCGA CCTGCGTGAG 
TACTATCCCA GTGTGGAGTG GGACATTCTC GGCGTTCCGG CTGAGCGGCA CGAGAAGTAC 
TATCCCTGCT GTGCCGAACC GTATCCGGAT ATCTTCTTCA ACATCACCCT GAGGCGAAAG 
ACTCTCTTCT ACACGGTTAA CCTGATTATT CCATGTGTGG GCATCTCGTA TCTTTCGGTG 
CTGGTCTTTT ACCTGCCCGC CGATTCTGGC GAGAAGATTG CTCTGTGCAT CAGCATCCTG 
CTGTCGCAAA CCATGTTCTT CCTGCTCATA TCGGAGATTA TACCATCGAC TTCACTGGCA 
TTGCCGCTAC TGGGAAAATA TCTACTGTTC ACCATGTTGC TGGTTGGCCT GAGTGTTGTC 
ATCACGATTA TCATACTAAA CATACACTAC CGGAAGCCGA GTACGCACAA GATGCGACCC 
TGGATTAGGT CCTTCTTCAT CAAGAGATTG CCGAAACTCC TGCTGATGCG TGTGCCCAAG 
GACCTGCTGC GTGACCTGGC GGCCAACAAG ATCAACTACG GCCTCAAGTT CAGCAAGACC 
AAGTTCGGAC AGGCGCTGAT GGACGAGATG CAAATGAACT CGGGCGGCTC CAGTCCAGAC 
TCCCTGCGGC GGATGCAAGG TCGTGTGGGT GCTGGTGGGT GCAATGGCAT GCACGTGACC 
ACGGCCACAA ACAGATTCAG CGGCTTGGTG GGGGCTTTGG GCGGCGGACT GAGCACCCTG 
AGCGGCTACA ACGGACTGCC ATCGGTGCTA TCCGGATTGG ACGACTCTTT GAGCGATGTG 
GCCGCACGCA AAAAGTATCC TTTCGAGCTG GAAAAGGCCA TCCACAACGT CATGTTCATA 
CAGCACCACA TGCAGCGGCA GGACGAGTTC AATGCGGAAG ATCAGGACTG GGGCTTTGTG 
GCCATGGTCA TGGATCGCCT ATTCCTCTGG CTCTTTATGA TCGCATCCTT GGTGGGCACA 
TTTGTGATCC TAGGCGAGGC TCCGTCGCTA TATGACGATA CCAAGGCCAT TGATGTTCAG 
CTATCCGATG TTGCCAAGCA AATCTACAAT CTAACCGAGA AGAAGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nAChRalpha2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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