nAChRalpha1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nAChRalpha1
Entrez Gene ID	42918
Full Name	nicotinic Acetylcholine Receptor alpha1
Synonyms	ALS,ALs,Acr96A,Acr96Aa,AcrB,CG5610,CG5610-PA,Dalpha1,Dmel\CG5610,NAcRa96Aa,alpha-like,alpha1,als,dALS,nAChR,nAChR-alpha96Aa,nAChRalpha-96Aa,nAcRalpha-96Aa,nAcRs,nAcr96Aa
General protein information	Preferred Names nicotinic Acetylcholine Receptor alpha1 Names CG5610 gene product from transcript CG5610-RC ALS CG5610-PA CG5610-PB CG5610-PC Dalpha1 alpha-like subunit alpha-like-subunit nAChRalpha1-PA nAChRalpha1-PB nAChRalpha1-PC nicotinic acetylcholine receptor alpha 96Aa nicotinic acetylcholine receptor alpha-96Aa nicotinic acetylcholine receptor alpha-like nicotinic acetylcholine receptor alpha1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96A1-96A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_079757.4	NP_524481.2	nicotinic acetylcholine receptor alpha1, isoform A
NM_001275987.1	NP_001262916.1	nicotinic acetylcholine receptor alpha1, isoform B
NM_001275988.2	NP_001262917.1	nicotinic acetylcholine receptor alpha1, isoform C

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Chrnb2	NP_062170.1
Danio rerio (zebrafish)	LOC101882975	XP_005174531.1
Mus musculus (house mouse)	Chrnb2	NP_033732.2
Gallus gallus (chicken)	CHRNB2	NP_990144.1
Canis lupus familiaris (dog)	CHRNB2	XP_547565.2
Drosophila melanogaster (fruit fly)	nAcRalpha-96Aa	NP_524481.2
Xenopus tropicalis (tropical clawed frog)	chrnb2	NP_001093684.1
Pan troglodytes (chimpanzee)	CHRNB2	XP_001152392.1
	CHRNB2	XP_001114439.1
Danio rerio (zebrafish)	chrnb2b	XP_005169811.1
Homo sapiens (human)	CHRNB2	NP_000739.1
Bos taurus (cattle)	CHRNB2	NP_001179403.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002974	XP_311918.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutation can confer nitenpyram resistance
Title: Mutations in Dalpha1 or Dbeta2 nicotinic acetylcholine receptor subunits can confer resistance to neonicotinoids in Drosophila melanogaster.

The following nAChRalpha1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nAChRalpha1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05958	NM_079757.4 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha1, transcript variant A (nAChRalpha1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05958	NM_001275988.2 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha1, transcript variant C (nAChRalpha1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa08728	NM_001275987.1 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha1, transcript variant B (nAChRalpha1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nAChRalpha1 ( NM_079757.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524481.2 Drosophila melanogaster nicotinic acetylcholine receptor alpha1, transcript variant A (nAChRalpha1), mRNA.

Price & Availability↑

CloneID	OFa05958
Clone ID Related Accession (Same CDS sequence)	NM_079757.4 , NM_001275988.2
Accession Version	NM_079757.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079757.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAGCG TGCTATTCGC AGCTGTATTC ATAGCATTAC ACTTTGCCAC CGGCGGCCTG 
GCCAACCCAG ATGCGAAGCG ACTCTACGAC GACCTGCTGA GCAACTACAA TCGCCTCATC 
CGACCCGTGG GCAACAACTC GGACCGTCTC ACCGTCAAGA TGGGTCTGCG CCTCTCCCAG 
CTGATCGATG TGAATCTAAA GAATCAAATT ATGACAACCA ATGTCTGGGT GGAACAGGAA 
TGGAACGACT ATAAATTGAA ATGGAATCCG GATGACTATG GCGGCGTGGA CACTTTGCAC 
GTTCCCTCCG AGCATATATG GCTACCGGAT ATTGTGCTCT ATAACAACGC CGATGGCAAC 
TATGAAGTGA CAATAATGAC AAAAGCAATT CTTCATCACA CGGGCAAAGT GGTGTGGAAA 
CCGCCCGCCA TTTACAAATC CTTCTGCGAA ATTGATGTCG AGTACTTTCC CTTCGACGAG 
CAGACATGTT TCATGAAGTT CGGATCCTGG ACCTACGATG GTTACATGGT GGACTTGAGG 
CACTTGAAGC AGACCGCCGA CTCGGACAAC ATCGAGGTGG GCATCGACCT GCAGGACTAC 
TACATCTCCG TGGAGTGGGA CATCATGCGT GTGCCGGCGG TGCGGAACGA GAAGTTCTAC 
AGCTGCTGCG AGGAGCCGTA TCTGGACATA GTGTTCAACC TGACGCTCCG CCGGAAGACG 
CTCTTCTACA CGGTCAACCT GATCATACCC TGCGTGGGCA TCTCGTTCCT GTCCGTTCTG 
GTCTTCTACC TGCCCAGCGA CTCTGGCGAG AAGATCTCGC TCTGCATCAG CATCCTGCTC 
TCGCTTACCG TGTTCTTCCT CCTGCTGGCC GAGATCATTC CGCCCACGTC GCTGACGGTG 
CCGCTGCTGG GAAAGTATCT GCTCTTCACC ATGATGCTGG TCACGCTCTC TGTCGTGGTC 
ACCATTGCCG TGCTCAATGT GAATTTTAGA TCCCCTGTCA CGCATCGCAT GGCACCGTGG 
GTGCAGCGCC TCTTCATCCA GATCCTGCCC AAGCTGCTCT GCATCGAGCG GCCCAAGAAG 
GAGGAGCCCG AGGAGGACCA GCCGCCCGAA GTGCTCACCG ATGTCTATCA CCTGCCGCCG 
GATGTGGACA AGTTTGTCAA CTACGATTCG AAGCGTTTCA GCGGCGACTA CGGCATTCCA 
GCACTACCCG CTTCGCATCG CTTCGATTTG GCTGCGGCGG GCGGGATTAG CGCCCACTGT 
TTCGCAGAAC CGCCGCTGCC CTCCTCACTG CCACTCCCGG GCGCCGACGA CGATCTGTTC 
AGCCCGTCGG GCCTCAACGG AGACATCAGT CCCGGCTGCT GCCCGGCCGC TGCAGCCGCC 
GCTGCCGCCG ATCTCAGTCC TACGTTCGAG AAGCCCTACG CCCGCGAAAT GGAGAAGACC 
ATCGAAGGAT CCCGCTTCAT CGCGCAGCAC GTGAAGAACA AGGATAAGTT TGAGAGTGTG 
GAAGAGGACT GGAAGTACGT TGCCATGGTA TTGGATCGTA TGTTTCTGTG GATCTTCGCG 
ATCGCTTGCG TGGTCGGCAC AGCGCTGATT ATCCTGCAGG CTCCCAGCTT GTACGACCAA 
TCGCAGCCGA TCGATATACT CTACTCGAAG ATTGCCAAGA AGAAGTTCGA GCTGCTCAAG 
ATGGGCAGCG AGAACACCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524481.2
CDS	1481..3184
Translation	MGSVLFAAVFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLPPDVDKFVNYDSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGLNGDISPGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDRMFLWIFAIACVVGTALIILQAPSLYDQSQPIDILYSKIAKKKFELLKMGSENTL

Target ORF information:

RefSeq Version	NM_079757.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha1, transcript variant A (nAChRalpha1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079757.4

ORF Insert Sequence:

ATGGGTAGCG TGCTATTCGC AGCTGTATTC ATAGCATTAC ACTTTGCCAC CGGCGGCCTG 
GCCAACCCAG ATGCGAAGCG ACTCTACGAC GACCTGCTGA GCAACTACAA TCGCCTCATC 
CGACCCGTGG GCAACAACTC GGACCGTCTC ACCGTCAAGA TGGGTCTGCG CCTCTCCCAG 
CTGATCGATG TGAATCTAAA GAATCAAATT ATGACAACCA ATGTCTGGGT GGAACAGGAA 
TGGAACGACT ATAAATTGAA ATGGAATCCG GATGACTATG GCGGCGTGGA CACTTTGCAC 
GTTCCCTCCG AGCATATATG GCTACCGGAT ATTGTGCTCT ATAACAACGC CGATGGCAAC 
TATGAAGTGA CAATAATGAC AAAAGCAATT CTTCATCACA CGGGCAAAGT GGTGTGGAAA 
CCGCCCGCCA TTTACAAATC CTTCTGCGAA ATTGATGTCG AGTACTTTCC CTTCGACGAG 
CAGACATGTT TCATGAAGTT CGGATCCTGG ACCTACGATG GTTACATGGT GGACTTGAGG 
CACTTGAAGC AGACCGCCGA CTCGGACAAC ATCGAGGTGG GCATCGACCT GCAGGACTAC 
TACATCTCCG TGGAGTGGGA CATCATGCGT GTGCCGGCGG TGCGGAACGA GAAGTTCTAC 
AGCTGCTGCG AGGAGCCGTA TCTGGACATA GTGTTCAACC TGACGCTCCG CCGGAAGACG 
CTCTTCTACA CGGTCAACCT GATCATACCC TGCGTGGGCA TCTCGTTCCT GTCCGTTCTG 
GTCTTCTACC TGCCCAGCGA CTCTGGCGAG AAGATCTCGC TCTGCATCAG CATCCTGCTC 
TCGCTTACCG TGTTCTTCCT CCTGCTGGCC GAGATCATTC CGCCCACGTC GCTGACGGTG 
CCGCTGCTGG GAAAGTATCT GCTCTTCACC ATGATGCTGG TCACGCTCTC TGTCGTGGTC 
ACCATTGCCG TGCTCAATGT GAATTTTAGA TCCCCTGTCA CGCATCGCAT GGCACCGTGG 
GTGCAGCGCC TCTTCATCCA GATCCTGCCC AAGCTGCTCT GCATCGAGCG GCCCAAGAAG 
GAGGAGCCCG AGGAGGACCA GCCGCCCGAA GTGCTCACCG ATGTCTATCA CCTGCCGCCG 
GATGTGGACA AGTTTGTCAA CTACGATTCG AAGCGTTTCA GCGGCGACTA CGGCATTCCA 
GCACTACCCG CTTCGCATCG CTTCGATTTG GCTGCGGCGG GCGGGATTAG CGCCCACTGT 
TTCGCAGAAC CGCCGCTGCC CTCCTCACTG CCACTCCCGG GCGCCGACGA CGATCTGTTC 
AGCCCGTCGG GCCTCAACGG AGACATCAGT CCCGGCTGCT GCCCGGCCGC TGCAGCCGCC 
GCTGCCGCCG ATCTCAGTCC TACGTTCGAG AAGCCCTACG CCCGCGAAAT GGAGAAGACC 
ATCGAAGGAT CCCGCTTCAT CGCGCAGCAC GTGAAGAACA AGGATAAGTT TGAGAGTGTG 
GAAGAGGACT GGAAGTACGT TGCCATGGTA TTGGATCGTA TGTTTCTGTG GATCTTCGCG 
ATCGCTTGCG TGGTCGGCAC AGCGCTGATT ATCCTGCAGG CTCCCAGCTT GTACGACCAA 
TCGCAGCCGA TCGATATACT CTACTCGAAG ATTGCCAAGA AGAAGTTCGA GCTGCTCAAG 
ATGGGCAGCG AGAACACCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha1 ( NM_001275988.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262917.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha1, transcript variant C (nAChRalpha1), mRNA.

Price & Availability↑

CloneID	OFa05958
Clone ID Related Accession (Same CDS sequence)	NM_079757.4 , NM_001275988.2
Accession Version	NM_001275988.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275988.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAGCG TGCTATTCGC AGCTGTATTC ATAGCATTAC ACTTTGCCAC CGGCGGCCTG 
GCCAACCCAG ATGCGAAGCG ACTCTACGAC GACCTGCTGA GCAACTACAA TCGCCTCATC 
CGACCCGTGG GCAACAACTC GGACCGTCTC ACCGTCAAGA TGGGTCTGCG CCTCTCCCAG 
CTGATCGATG TGAATCTAAA GAATCAAATT ATGACAACCA ATGTCTGGGT GGAACAGGAA 
TGGAACGACT ATAAATTGAA ATGGAATCCG GATGACTATG GCGGCGTGGA CACTTTGCAC 
GTTCCCTCCG AGCATATATG GCTACCGGAT ATTGTGCTCT ATAACAACGC CGATGGCAAC 
TATGAAGTGA CAATAATGAC AAAAGCAATT CTTCATCACA CGGGCAAAGT GGTGTGGAAA 
CCGCCCGCCA TTTACAAATC CTTCTGCGAA ATTGATGTCG AGTACTTTCC CTTCGACGAG 
CAGACATGTT TCATGAAGTT CGGATCCTGG ACCTACGATG GTTACATGGT GGACTTGAGG 
CACTTGAAGC AGACCGCCGA CTCGGACAAC ATCGAGGTGG GCATCGACCT GCAGGACTAC 
TACATCTCCG TGGAGTGGGA CATCATGCGT GTGCCGGCGG TGCGGAACGA GAAGTTCTAC 
AGCTGCTGCG AGGAGCCGTA TCTGGACATA GTGTTCAACC TGACGCTCCG CCGGAAGACG 
CTCTTCTACA CGGTCAACCT GATCATACCC TGCGTGGGCA TCTCGTTCCT GTCCGTTCTG 
GTCTTCTACC TGCCCAGCGA CTCTGGCGAG AAGATCTCGC TCTGCATCAG CATCCTGCTC 
TCGCTTACCG TGTTCTTCCT CCTGCTGGCC GAGATCATTC CGCCCACGTC GCTGACGGTG 
CCGCTGCTGG GAAAGTATCT GCTCTTCACC ATGATGCTGG TCACGCTCTC TGTCGTGGTC 
ACCATTGCCG TGCTCAATGT GAATTTTAGA TCCCCTGTCA CGCATCGCAT GGCACCGTGG 
GTGCAGCGCC TCTTCATCCA GATCCTGCCC AAGCTGCTCT GCATCGAGCG GCCCAAGAAG 
GAGGAGCCCG AGGAGGACCA GCCGCCCGAA GTGCTCACCG ATGTCTATCA CCTGCCGCCG 
GATGTGGACA AGTTTGTCAA CTACGATTCG AAGCGTTTCA GCGGCGACTA CGGCATTCCA 
GCACTACCCG CTTCGCATCG CTTCGATTTG GCTGCGGCGG GCGGGATTAG CGCCCACTGT 
TTCGCAGAAC CGCCGCTGCC CTCCTCACTG CCACTCCCGG GCGCCGACGA CGATCTGTTC 
AGCCCGTCGG GCCTCAACGG AGACATCAGT CCCGGCTGCT GCCCGGCCGC TGCAGCCGCC 
GCTGCCGCCG ATCTCAGTCC TACGTTCGAG AAGCCCTACG CCCGCGAAAT GGAGAAGACC 
ATCGAAGGAT CCCGCTTCAT CGCGCAGCAC GTGAAGAACA AGGATAAGTT TGAGAGTGTG 
GAAGAGGACT GGAAGTACGT TGCCATGGTA TTGGATCGTA TGTTTCTGTG GATCTTCGCG 
ATCGCTTGCG TGGTCGGCAC AGCGCTGATT ATCCTGCAGG CTCCCAGCTT GTACGACCAA 
TCGCAGCCGA TCGATATACT CTACTCGAAG ATTGCCAAGA AGAAGTTCGA GCTGCTCAAG 
ATGGGCAGCG AGAACACCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262917.1
CDS	1481..3184
Translation	MGSVLFAAVFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLPPDVDKFVNYDSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGLNGDISPGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDRMFLWIFAIACVVGTALIILQAPSLYDQSQPIDILYSKIAKKKFELLKMGSENTL

Target ORF information:

RefSeq Version	NM_001275988.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha1, transcript variant C (nAChRalpha1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275988.2

ORF Insert Sequence:

ATGGGTAGCG TGCTATTCGC AGCTGTATTC ATAGCATTAC ACTTTGCCAC CGGCGGCCTG 
GCCAACCCAG ATGCGAAGCG ACTCTACGAC GACCTGCTGA GCAACTACAA TCGCCTCATC 
CGACCCGTGG GCAACAACTC GGACCGTCTC ACCGTCAAGA TGGGTCTGCG CCTCTCCCAG 
CTGATCGATG TGAATCTAAA GAATCAAATT ATGACAACCA ATGTCTGGGT GGAACAGGAA 
TGGAACGACT ATAAATTGAA ATGGAATCCG GATGACTATG GCGGCGTGGA CACTTTGCAC 
GTTCCCTCCG AGCATATATG GCTACCGGAT ATTGTGCTCT ATAACAACGC CGATGGCAAC 
TATGAAGTGA CAATAATGAC AAAAGCAATT CTTCATCACA CGGGCAAAGT GGTGTGGAAA 
CCGCCCGCCA TTTACAAATC CTTCTGCGAA ATTGATGTCG AGTACTTTCC CTTCGACGAG 
CAGACATGTT TCATGAAGTT CGGATCCTGG ACCTACGATG GTTACATGGT GGACTTGAGG 
CACTTGAAGC AGACCGCCGA CTCGGACAAC ATCGAGGTGG GCATCGACCT GCAGGACTAC 
TACATCTCCG TGGAGTGGGA CATCATGCGT GTGCCGGCGG TGCGGAACGA GAAGTTCTAC 
AGCTGCTGCG AGGAGCCGTA TCTGGACATA GTGTTCAACC TGACGCTCCG CCGGAAGACG 
CTCTTCTACA CGGTCAACCT GATCATACCC TGCGTGGGCA TCTCGTTCCT GTCCGTTCTG 
GTCTTCTACC TGCCCAGCGA CTCTGGCGAG AAGATCTCGC TCTGCATCAG CATCCTGCTC 
TCGCTTACCG TGTTCTTCCT CCTGCTGGCC GAGATCATTC CGCCCACGTC GCTGACGGTG 
CCGCTGCTGG GAAAGTATCT GCTCTTCACC ATGATGCTGG TCACGCTCTC TGTCGTGGTC 
ACCATTGCCG TGCTCAATGT GAATTTTAGA TCCCCTGTCA CGCATCGCAT GGCACCGTGG 
GTGCAGCGCC TCTTCATCCA GATCCTGCCC AAGCTGCTCT GCATCGAGCG GCCCAAGAAG 
GAGGAGCCCG AGGAGGACCA GCCGCCCGAA GTGCTCACCG ATGTCTATCA CCTGCCGCCG 
GATGTGGACA AGTTTGTCAA CTACGATTCG AAGCGTTTCA GCGGCGACTA CGGCATTCCA 
GCACTACCCG CTTCGCATCG CTTCGATTTG GCTGCGGCGG GCGGGATTAG CGCCCACTGT 
TTCGCAGAAC CGCCGCTGCC CTCCTCACTG CCACTCCCGG GCGCCGACGA CGATCTGTTC 
AGCCCGTCGG GCCTCAACGG AGACATCAGT CCCGGCTGCT GCCCGGCCGC TGCAGCCGCC 
GCTGCCGCCG ATCTCAGTCC TACGTTCGAG AAGCCCTACG CCCGCGAAAT GGAGAAGACC 
ATCGAAGGAT CCCGCTTCAT CGCGCAGCAC GTGAAGAACA AGGATAAGTT TGAGAGTGTG 
GAAGAGGACT GGAAGTACGT TGCCATGGTA TTGGATCGTA TGTTTCTGTG GATCTTCGCG 
ATCGCTTGCG TGGTCGGCAC AGCGCTGATT ATCCTGCAGG CTCCCAGCTT GTACGACCAA 
TCGCAGCCGA TCGATATACT CTACTCGAAG ATTGCCAAGA AGAAGTTCGA GCTGCTCAAG 
ATGGGCAGCG AGAACACCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha1 ( NM_001275987.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262916.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha1, transcript variant B (nAChRalpha1), mRNA.

Price & Availability↑

CloneID	OFa08728
Clone ID Related Accession (Same CDS sequence)	NM_001275987.1
Accession Version	NM_001275987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAGCG TGCTATTCGC AGCTGTATTC ATAGCATTAC ACTTTGCCAC CGGCGGCCTG 
GCCAACCCAG ATGCGAAGCG ACTCTACGAC GACCTGCTGA GCAACTACAA TCGCCTCATC 
CGACCCGTGG GCAACAACTC GGACCGTCTC ACCGTCAAGA TGGGTCTGCG CCTCTCCCAG 
CTGATCGATG TGAATCTAAA GAATCAAATT ATGACAACCA ATGTCTGGGT GGAACAGGAA 
TGGAACGACT ATAAATTGAA ATGGAATCCG GATGACTATG GCGGCGTGGA CACTTTGCAC 
GTTCCCTCCG AGCATATATG GCTACCGGAT ATTGTGCTCT ATAACAACGC CGATGGCAAC 
TATGAAGTGA CAATAATGAC AAAAGCAATT CTTCATCACA CGGGCAAAGT GGTGTGGAAA 
CCGCCCGCCA TTTACAAATC CTTCTGCGAA ATTGATGTCG AGTACTTTCC CTTCGACGAG 
CAGACATGTT TCATGAAGTT CGGATCCTGG ACCTACGATG GTTACATGGT GGACTTGAGG 
CACTTGAAGC AGACCGCCGA CTCGGACAAC ATCGAGGTGG GCATCGACCT GCAGGACTAC 
TACATCTCCG TGGAGTGGGA CATCATGCGT GTGCCGGCGG TGCGGAACGA GAAGTTCTAC 
AGCTGCTGCG AGGAGCCGTA TCTGGACATA GTGTTCAACC TGACGCTCCG CCGGAAGACG 
CTCTTCTACA CGGTCAACCT GATCATACCC TGCGTGGGCA TCTCGTTCCT GTCCGTTCTG 
GTCTTCTACC TGCCCAGCGA CTCTGGCGAG AAGATCTCGC TCTGCATCAG CATCCTGCTC 
TCGCTTACCG TGTTCTTCCT CCTGCTGGCC GAGATCATTC CGCCCACGTC GCTGACGGTG 
CCGCTGCTGG GAAAGTATCT GCTCTTCACC ATGATGCTGG TCACGCTCTC TGTCGTGGTC 
ACCATTGCCG TGCTCAATGT GAATTTTAGA TCCCCTGTCA CGCATCGCAT GGCACCGTGG 
GTGCAGCGCC TCTTCATCCA GATCCTGCCC AAGCTGCTCT GCATCGAGCG GCCCAAGAAG 
GAGGAGCCCG AGGAGGACCA GCCGCCCGAA GTGCTCACCG ATGTCTATCA CCTGCCGCCG 
GATGTGGACA AGTTTGTCAA CTACGATTCG AAGCGTTTCA GCGGCGACTA CGGCATTCCA 
GCACTACCCG CTTCGCATCG CTTCGATTTG GCTGCGGCGG GCGGGATTAG CGCCCACTGT 
TTCGCAGAAC CGCCGCTGCC CTCCTCACTG CCACTCCCGG GCGCCGACGA CGATCTGTTC 
AGCCCGTCGG GCCTCAACGG AGACATCAGT CCCGGCTGCT GCCCGGCCGC TGCAGCCGCC 
GCTGCCGCCG ATCTCAGTCC TACGTTCGAG AAGCCCTACG CCCGCGAAAT GGAGAAGACC 
ATCGAAGGAT CCCGCTTCAT CGCGCAGCAC GTGAAGAACA AGGATAAGTT TGAGAGTGTG 
GAAGAGGACT GGAAGTACGT TGCCATGGTA TTGGATCGTA TGTTTCTGTG GATCTTCGCG 
ATCGCTTGCG TGGTCGGCAC AGCGCTGATT ATCCTGCAGG CTCCCAGCTT GTACGACCAA 
TCGCAGCCGA TCGATATACT CTACTCGAAG ATTGCCAAGA AGAAGTTCGA GCTGCTCAAG 
ATGGGCAGCG AGAACACCTT ATAGCGACCA CTTGGGTTCG CGGCTGGCTG GTGGAATCTA 
ATCATCAGCT CGCTGCGGAC CCTGTTCGCC GCTCGCGACG ATGATCGTGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262916.1
CDS	1481..3274
Translation	MGSVLFAAVFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLPPDVDKFVNYDSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGLNGDISPGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDRMFLWIFAIACVVGTALIILQAPSLYDQSQPIDILYSKIAKKKFELLKMGSENTL*RPLGFAAGWWNLIISSLRTLFAARDDDRG

Target ORF information:

RefSeq Version	NM_001275987.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha1, transcript variant B (nAChRalpha1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275987.1

ORF Insert Sequence:

ATGGGTAGCG TGCTATTCGC AGCTGTATTC ATAGCATTAC ACTTTGCCAC CGGCGGCCTG 
GCCAACCCAG ATGCGAAGCG ACTCTACGAC GACCTGCTGA GCAACTACAA TCGCCTCATC 
CGACCCGTGG GCAACAACTC GGACCGTCTC ACCGTCAAGA TGGGTCTGCG CCTCTCCCAG 
CTGATCGATG TGAATCTAAA GAATCAAATT ATGACAACCA ATGTCTGGGT GGAACAGGAA 
TGGAACGACT ATAAATTGAA ATGGAATCCG GATGACTATG GCGGCGTGGA CACTTTGCAC 
GTTCCCTCCG AGCATATATG GCTACCGGAT ATTGTGCTCT ATAACAACGC CGATGGCAAC 
TATGAAGTGA CAATAATGAC AAAAGCAATT CTTCATCACA CGGGCAAAGT GGTGTGGAAA 
CCGCCCGCCA TTTACAAATC CTTCTGCGAA ATTGATGTCG AGTACTTTCC CTTCGACGAG 
CAGACATGTT TCATGAAGTT CGGATCCTGG ACCTACGATG GTTACATGGT GGACTTGAGG 
CACTTGAAGC AGACCGCCGA CTCGGACAAC ATCGAGGTGG GCATCGACCT GCAGGACTAC 
TACATCTCCG TGGAGTGGGA CATCATGCGT GTGCCGGCGG TGCGGAACGA GAAGTTCTAC 
AGCTGCTGCG AGGAGCCGTA TCTGGACATA GTGTTCAACC TGACGCTCCG CCGGAAGACG 
CTCTTCTACA CGGTCAACCT GATCATACCC TGCGTGGGCA TCTCGTTCCT GTCCGTTCTG 
GTCTTCTACC TGCCCAGCGA CTCTGGCGAG AAGATCTCGC TCTGCATCAG CATCCTGCTC 
TCGCTTACCG TGTTCTTCCT CCTGCTGGCC GAGATCATTC CGCCCACGTC GCTGACGGTG 
CCGCTGCTGG GAAAGTATCT GCTCTTCACC ATGATGCTGG TCACGCTCTC TGTCGTGGTC 
ACCATTGCCG TGCTCAATGT GAATTTTAGA TCCCCTGTCA CGCATCGCAT GGCACCGTGG 
GTGCAGCGCC TCTTCATCCA GATCCTGCCC AAGCTGCTCT GCATCGAGCG GCCCAAGAAG 
GAGGAGCCCG AGGAGGACCA GCCGCCCGAA GTGCTCACCG ATGTCTATCA CCTGCCGCCG 
GATGTGGACA AGTTTGTCAA CTACGATTCG AAGCGTTTCA GCGGCGACTA CGGCATTCCA 
GCACTACCCG CTTCGCATCG CTTCGATTTG GCTGCGGCGG GCGGGATTAG CGCCCACTGT 
TTCGCAGAAC CGCCGCTGCC CTCCTCACTG CCACTCCCGG GCGCCGACGA CGATCTGTTC 
AGCCCGTCGG GCCTCAACGG AGACATCAGT CCCGGCTGCT GCCCGGCCGC TGCAGCCGCC 
GCTGCCGCCG ATCTCAGTCC TACGTTCGAG AAGCCCTACG CCCGCGAAAT GGAGAAGACC 
ATCGAAGGAT CCCGCTTCAT CGCGCAGCAC GTGAAGAACA AGGATAAGTT TGAGAGTGTG 
GAAGAGGACT GGAAGTACGT TGCCATGGTA TTGGATCGTA TGTTTCTGTG GATCTTCGCG 
ATCGCTTGCG TGGTCGGCAC AGCGCTGATT ATCCTGCAGG CTCCCAGCTT GTACGACCAA 
TCGCAGCCGA TCGATATACT CTACTCGAAG ATTGCCAAGA AGAAGTTCGA GCTGCTCAAG 
ATGGGCAGCG AGAACACCTT ATAGCGACCA CTTGGGTTCG CGGCTGGCTG GTGGAATCTA 
ATCATCAGCT CGCTGCGGAC CCTGTTCGCC GCTCGCGACG ATGATCGTGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nAChRalpha1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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