Root cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Root
Entrez Gene ID	42860
Full Name	Rootletin
Synonyms	CG6129,Dmel\CG6129,Q8IMX3_DROME
General protein information	Preferred Names Rootletin Names CG6129 gene product from transcript CG6129-RE CG6129-PD CG6129-PE CG6129-PF Root-PD Root-PE Root-PF rootletin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95E1-95E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300568.1	NP_001287497.1	rootletin, isoform E
NM_142959.4	NP_651216.2	rootletin, isoform D
NM_176553.2	NP_788730.2	rootletin, isoform F

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003449	XP_311738.5
Pan troglodytes (chimpanzee)	CROCC	XP_513109.2
Canis lupus familiaris (dog)	CROCC	XP_544542.4
Xenopus tropicalis (tropical clawed frog)	LOC100498193	XP_002933382.2
Homo sapiens (human)	CROCC	NP_055490.3
Mus musculus (house mouse)	Crocc	NP_742120.2
Danio rerio (zebrafish)	LOC101885282	XP_005166013.1
Drosophila melanogaster (fruit fly)	Rootletin	NP_651216.2
Bos taurus (cattle)	CROCC	XP_002685844.2
Rattus norvegicus (Norway rat)	Crocc	NP_001101460.2
Gallus gallus (chicken)	CROCC	XP_425750.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila melanogaster Rootletin, the sole orthologue of the mammalian paralogs Rootletin and C-Nap1, assembles into rootlets of diverse lengths among sensory neuron subtypes.
Title: Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila.

The following Root gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Root cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05895	NM_142959.4 Latest version!	Drosophila melanogaster rootletin, transcript variant D (Root), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa05895	NM_176553.2 Latest version!	Drosophila melanogaster rootletin, transcript variant F (Root), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa05895	NM_001300568.1 Latest version!	Drosophila melanogaster rootletin, transcript variant E (Root), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Root ( NM_001300568.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287497.1 Drosophila melanogaster rootletin, transcript variant E (Root), mRNA.

Price & Availability↑

CloneID	OFa05895
Clone ID Related Accession (Same CDS sequence)	NM_001300568.1 , NM_142959.4 , NM_176553.2
Accession Version	NM_001300568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rootletin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGCGT ATCGCGATAA CTTCAAGCAA ACGCCCTGCC CATCGGCCGT CGACCTTCAG 
GCGGCGGGAC CAGCCCACCA ATCGGCCACC GCCTCTCGGC TGCCCTTCTC GCGCAGCCAG 
CGTGGGCGTG ACCCCTCGGC AAGTGGCGCC TCAGTTTCCG CCTCCGTTGC TGGTGGTCTA 
ACGCCCAGGA TGATGAGTCC GGGTCCGCCG GGCGCCGGAG GAGGAGGAGG AGGAGGAGTG 
AGCGGCGGCG GCAGTGGAGA TCCCTCGGCC CTGTTGCGCC AGAATCAGGA GCTGCGCCAA 
CGGCTGGCCG ACGAGTCGCA TAGCTATCGA CGCCGCCTGG ACACCTACAA GCAGGCGCAG 
CACAACCAGG CCAACTTGGT CAGCCGGCTG CAGTCGAAGA TCCAGCAGTA TCGACAGCGG 
TGTAGCGACC TGGAGGAGCG CATGCACGAG ACCATTAAGC CGACGGCGGG AGTAGGACCC 
AAGCTGACCA CGGGTCCCAC CAACCAAGTG CTGTGTTCAA CTTCTTTAAC TCTGGGACAG 
AGCAGCTTGC CCTGCAGCTC CTCACTGGAC TCGCCGCCGC CCAGTTGCAG TCGTGACTAT 
GTTGACGATG TCTTGGTCAC CGGAGGCGGC GCCGGTGCGG CGGAACTGTG CCGCAAACTG 
GAGGAGGAGC ACCAGCGCTG CGAGCAGATT GTCGCCCAGA ACAGCGCACT GCGTCAGCAG 
CTGGAGGAGT CGAATCGCAC CAACGAGGCG CTCACCAACG ACCTACAGAA GCTGACCAAC 
GACTGGGCGA GTCTGCGGGA TGAGCTGCTG ATCAAGGAGG ATGAGTTCAA GGAGGAGGAG 
CAGGCCTTCA AGGACTACTA CAACAGCGAG CACAATCGCC TGCTGAAGAT GTGGCGCGAA 
GTTGTGGCCG TCAAGAGATC CTTCAAGGAG ATGCAGACGG CCATGAAGGC GGAGGTAGCC 
AAGATGGGTC AGGAGATCAA TTGTGTGGGC AAGGACATCA ATGGCTCCAA CGCAACGGTC 
GCCTTTGCCG TCCAGCAAGC CAAGCGGGCT GCGGATGATG AACTGAAGCA ATCGCAGCGC 
AGCAACGATG AACTCCAGAA TCAATTGGCC ACCCTGAAGG TGCAGTACGA GAGTGCCCGG 
CACGAGATCA TGGAGCGGGA TCAGCGACTA CTGGAGCTAA TGAATCAGTT GAAGAAGCTG 
GAGGATCGCT GCGCCCAAGC CGAATCCCAA GCAGCTCTGG CCAGTCGCTA TAGCGACGAG 
ATCGAGCGAC TGAACAATTC CATGCGAGAA ATCGCGCAGG CCGTCGTTCA AGATGCTGAG 
AACGCAGATC GCGAAGCAGA CGCCGAGGTC ACCGGCGGTG TCATGCAGCA CATGCACCTC 
ACGCGTGACG CCGCCTCTGT TGTGGGCGGA GCAGGTGGAG CGGGCAGCAC CGCCGGCGGC 
GGAGGGAAAT CACCGCGTCG CAACTCGACA CGCGCCTCTC AAGCCTTCGC CGAGGGCACC 
ATCTCAGCCG TCCAGGCGGC GCTCCACAAA TACCAGCTGG CCCTGCACGA CATGCAGGTG 
AAATTCCAGA ACACCAGCGA GACCCTGCGC ACCACCAAGG CCCAGCTGGA GACCAGCGAG 
GGTACCAAAC AGCTGCTGAC CACCAAGATG CAGCAGCTCA CCGAGAAACT GGACAGCAGC 
AACTCCAAGC TATCGGAATT GCTGCAGGAA AGGGAGAGTC TGCAGCGCGG ACTGGACGAT 
ATCCGTGTCC AGAAGCAGCA GTCTGAGATG GGACGAGCCG ATATCAATAG TGCGTTCGAG 
AATCTGAGCA GTGATTATGA GAAGATGCAG CTGAACTGTG GTAAACTCCA AAAACGTATC 
GATTCCATGG AGGAGGACAA AAAGGCAGTG GAGCTGGAGA TCCAACGTAT ACTGAAGGAC 
AAGAACATAA CCGAGTTGAA TTTGAGGTCT GAGGAAGATC GCAGTAGTCG TTTGCGGGAG 
GAAACCATAT CCTTGCGCGA GGAGCTTAAC CGAGTGAGCC TGAATCGTGA TCTTCTCGAG 
CAGCAGCGCA TCGAGTCCGA TAATTTGATC AATCTGCTCG AGAAACAGAA GTCCGACCTG 
GAGTACGATC TGGACAAGCT GTTGCTGGAG AAGTGCGATC TGCAGGAGAA GCACGAGAAG 
CTATCCAACA ATAGCTGCTC CACCAGTGAT GAGCTGAAGA GCGTTCAAAA TTGCCTTCAG 
GAGGCGCAGG AGGAGCGCAA GAAGCTCCGT ATTCAGTCCG TCGATCAGTG CAATGAAATC 
GGAGAGCTTA AGAAGGAGCT GGCGATACTG GACAAGGCAC GACTCGAACT GGAGACGGAC 
AATCTGTCGG CTGGGGAAAA GCTCAAGTGT CTGCAGCTGG AGAAGGAGAA AATTCTGCAA 
GACTTGGCCT GCGTCACCCG GGATCGTGGT GACATCCACA ATCAGCTAAC GGCGATGTGT 
CGCAAAAAGG AGGCTCTGAA TGAGGAACTT ATGCGGACTC GGCAGCGTCT GGAGCAAACC 
ACCGAGACCA ATAGCCGGCT GAATAGAAAT CTGGAGGAGA TGGTGAAGGA TGTGGAGGAG 
AAGCAAGTGG TCATCGATCT GCACGAGAAG GACACACATC GCTTGAACGA ACTCCTGGCC 
GCCCTGCGTT CGGAGAAGGA ATCCCTGGAA TCGGTGCTCT TCGATACAAA CACCTCACTG 
GAGGCCACCG AGGAGCGACG CAGTCAGCTG GAGCGGGATC TGCAGGAGGC TCTGGTGCGT 
GAGGAGAGCC TAAAGAATCA TGTGGCTCGC TTGCAAAAGG AGCTGGAGCA GTGTCAGCGC 
AAGGCCCAAG AGACCAAGAC GCAGCTGCTT AACGCCGCCC GTGCGGCTGA GAGTGACTTC 
AACCAGAAAA TCGCCAATCT GCAGGCTTGT GCAGAGGAGG CGGCCAAACG ACATGGCGAG 
GAGATTCTAC AGTTGCGAAA TGCCTTGGAG AAGCGAATGC AACAGGCTCT GCAAGCGTTG 
CAGACGGCCA AGGATGATGA GATCGAGAAG TTGCAGGAGC GTCTGGCCAC CTTGCAGGCG 
CATCTCGAGA GCCTTGTCCA GCAGCATGAG GAGGCACTGA TTCGGGCGGA GAGCGAGAAG 
CAGCAAGCCC TTTTGATTGC CCACCGGGAT AAGCAAGCGG TGGCCGAGCG TTTGGAGGCC 
GTATCCCGGG ATCTCAAGAC CGAACAGGAG TCCCTCGACC GGAGCAGACG GGAGGCCAAT 
GCGCGCGATG AGAAGCAGAG GGCTGCCATT GCCCAGCTGA AGGACGAGAT GGTGCAGATG 
CGCACCAAGG AGGAGGAGCA CAAGATTAAG TTGGAGGAAT GCATCCGGAA GCAGGAGCTG 
CAGTTGAGCA GCTTGCGCGA AGAACGCGAA TCCTTGTGCC GTGTGAGTGA GGAACTAAAG 
ATGGAGATTC GTCTGAAGGA GGACAGAATG GAGAGCACCA ACAATGAGTT GCAAGATGCG 
CTGCGCAAGT CCAAGGAGGG TGAGGGCTTC ATCGATAGCC TGCGCAAAGA GTTGACCGAC 
TGTCGCCGCC AACTGGCGGA CAGCAACATC GAGCGGGACA AGTATTCCGG CAGCAACAAG 
GAGCTGCGCG ACCACGTCAA GCGTGTGGAG AGCGCCAAGC GGGAGCAGGC GCGCGCCATC 
GAGGAGGCTC TGCAGAAGAT CAGCAATCTG GAGGATACCA AGAACTCGTT GGAGAACGAA 
CGCACTCGAT TGAGCACCAT ACTGAAGGAG ACGGAGAATC ACTTTACAAA GACCACCCAG 
GATCTGAATG CTACCAAGGC GCAGCTGCAG AAGGCTCAAG TGGAGTTCGC CCAGAAGGAC 
GAGGGCGGCA AGGAGTTGCA GTGCAAGCTG GTCGCCGAGG TGGAATTGAA GGAGCGGGCA 
CAGCAGGAGC TCTGCCAGAT TAAGAAGCAA TTATCGGATC TGGAAGCCAA TCTGTGTGCC 
ACTCGCCAGG AATTGGGCAG GGCTCGGTGT CAGAACAACC AGGAGGAGCA TCGCTTCCAT 
GCCAGGGAGC AGGAGTTGGC CCAGCGCTTG GAGGAGGGTC GTGGTAGGGA GAAGCGCCTG 
GAGGATCAGA AGCACAACCT GGAGGTCTGC CTAGCCGATG CCACTCAGCA GATCCAGGAG 
TTGAAGGCCC GTTTGGGCGG CGCCGAAGGT CGCATCCGTG CTTTGGATGA GCAGTTGTCC 
TGCGTGGAAC TACACAAGCG GGATACCGAA CAGAAGCTAT CCTCGGTGGT TCACACTCTG 
CGCCGGATTG CTGGCATCCA AGTGGACGGC AGTGTAAATT TGTCTCATCG CTTGCTGAGT 
CCCTCGCGAA GATTCAGTCC GTCTCGCAGC TGCGGAGACT ATGACAACAG GAGCACATCA 
CAATGCCCAG ATGGACCAAT TGATGTGGAC CCGGATCTGG TTAGGAAGGG TGTCCGCAAC 
CTGATGCATC AGGTGGCTCA GTTGGAGCGC GAGAAGGATG ACTACAAATC CCAATTGGGA 
GCAGCTAAGA AGCAACTCCA AGATGCTGCT GACCAGCAGC TACGATGCGA TGCCAAGCTG 
GGCAAACTGC AGGCCATGCT AAGAAATCTC CAAGAGGAGA AGAGCAATCT GGAAACGGAC 
CGCAAGATGA AAATCTCCGC CATCCAGGCG CTAGAGGAGA AGCTCAAGCA TCGCAACGAT 
GAGTGTCAGA TGCTAAGAGA ACGTTTGGCC CAAACAGAGA TGCAACTGGC TGCCACATCC 
GAGGAGAATG GCCAGAACGA GGAACGACTG GAGAAGAGCC GACAGCAGTG CTCCAAACTG 
GACAATGAGA AGCGCCAGCT GCAGGAGGAA TTGGCCAAGG TTGAGGGCCG GGCCAGCAAA 
CTCGAACTGC AGCGTGTTGC CATGGAGGGC GATCTTACCA GGCTACAAAT GGCCCTACAA 
GAGAAGGACT GCAGCATTCG TCAAATGGCT GAGCGGCTGG AGAACCAAAA CCGTGCCTTG 
ACCCAATTGG AGGATCGCTG CACCGCCCTT AAGTCCACCG TCGATCAGCT GAAGGAGCGC 
CTCCAGAAGT CCGCTGTGAG TGAAACCCAA CTGCGGGGCG AGATAAAGAC GCTCCAAAAG 
GAGCTTTCCG AGCAGGGTCA CTGCTCCCAG GCCAACGAGG ATAAGCTGAA GTTGGTGCAA 
AAGTCTCTGC AGACCGCTGA GAACGAGAAG CGCATCCTCA CCGAGCGCCT AGATAGCGCT 
CAGACCAATC TCAACGAGCT GCGACGTAGC CAACAGGCCC AGCTGGATGG TAACCAGCGC 
CTGCAGGAAC AGGTGACCGA TCTGGAGGTT CAGCGTTCGG CACTGGAGTC CCAACTTCGG 
ATTGCCAAGT GGAACCAGGA GAGTGGCGGC GACAAGGATC TGACAAACGG CAACGGAGGC 
GGAAATGGCG AGGAGGAGCT CAGCAGGCAG CTGAAGTCCT CGCAGCGGGA AAAGTCGGAG 
CTACGCAGCA AACTGCAAAC CCTACAGGAC AAAGTCAAAC AGTTGGAGTG CGACCGGAAA 
AGCAAGTTTT CGGGTGGAAA CGCCTATGAT CGGGCTGAGA AGTCCAACTC CTTTTACGGT 
GGCGCAGCTG AATCCGGCGA GTTCGACTCC AATCGCTACG ATGTGGGTGG AGGAAATGCT 
GGCGGAGGCT CTTTCAACTG CGGATTGGAT CACAGTGTAA TCGAGCAGGA GACGCGCGAT 
CTGCGACTCA AGGTGCGCCG CCTGGAGACA TTGTTGGCGG AGAAGGAGTC CGAGTTGGCG 
CGCTGCAAGG CGCGAATGAA CGACAGCGCC AAGTGCCATG ATGGCTTAGA TGGAGATCGT 
TATCGCAGTG CTCAGATGCA CGCAGAGAAG CTTCTTGACG CCAGGGAGCA GTCGCATCGG 
CAACAAGTGC TGCGCCTGGA GAACCAGATC TCAATGCTGC GCGAGCAGTT GGCCCAGGAG 
GCCAAACGGC GACAGCAGTA CATTCTACGC AGCTCGAAGG CCAATAGAGA AATGCAGCAT 
CTGAGGAGCA CCCTAGGAGA TTCCCTGCGC AATGTCTCCC AGCACCCGGT GGATCCCCAT 
CTCTTGGAAA GCGAGAGCCG GCGTCTCGAC TCGGCCGTTT CAATGAGCCT GCCACCGTCG 
AGCTGCCGGG ACTATGATCG CGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287497.1
CDS	404..6550
Translation	MQAYRDNFKQTPCPSAVDLQAAGPAHQSATASRLPFSRSQRGRDPSASGASVSASVAGGLTPRMMSPGPPGAGGGGGGGVSGGGSGDPSALLRQNQELRQRLADESHSYRRRLDTYKQAQHNQANLVSRLQSKIQQYRQRCSDLEERMHETIKPTAGVGPKLTTGPTNQVLCSTSLTLGQSSLPCSSSLDSPPPSCSRDYVDDVLVTGGGAGAAELCRKLEEEHQRCEQIVAQNSALRQQLEESNRTNEALTNDLQKLTNDWASLRDELLIKEDEFKEEEQAFKDYYNSEHNRLLKMWREVVAVKRSFKEMQTAMKAEVAKMGQEINCVGKDINGSNATVAFAVQQAKRAADDELKQSQRSNDELQNQLATLKVQYESARHEIMERDQRLLELMNQLKKLEDRCAQAESQAALASRYSDEIERLNNSMREIAQAVVQDAENADREADAEVTGGVMQHMHLTRDAASVVGGAGGAGSTAGGGGKSPRRNSTRASQAFAEGTISAVQAALHKYQLALHDMQVKFQNTSETLRTTKAQLETSEGTKQLLTTKMQQLTEKLDSSNSKLSELLQERESLQRGLDDIRVQKQQSEMGRADINSAFENLSSDYEKMQLNCGKLQKRIDSMEEDKKAVELEIQRILKDKNITELNLRSEEDRSSRLREETISLREELNRVSLNRDLLEQQRIESDNLINLLEKQKSDLEYDLDKLLLEKCDLQEKHEKLSNNSCSTSDELKSVQNCLQEAQEERKKLRIQSVDQCNEIGELKKELAILDKARLELETDNLSAGEKLKCLQLEKEKILQDLACVTRDRGDIHNQLTAMCRKKEALNEELMRTRQRLEQTTETNSRLNRNLEEMVKDVEEKQVVIDLHEKDTHRLNELLAALRSEKESLESVLFDTNTSLEATEERRSQLERDLQEALVREESLKNHVARLQKELEQCQRKAQETKTQLLNAARAAESDFNQKIANLQACAEEAAKRHGEEILQLRNALEKRMQQALQALQTAKDDEIEKLQERLATLQAHLESLVQQHEEALIRAESEKQQALLIAHRDKQAVAERLEAVSRDLKTEQESLDRSRREANARDEKQRAAIAQLKDEMVQMRTKEEEHKIKLEECIRKQELQLSSLREERESLCRVSEELKMEIRLKEDRMESTNNELQDALRKSKEGEGFIDSLRKELTDCRRQLADSNIERDKYSGSNKELRDHVKRVESAKREQARAIEEALQKISNLEDTKNSLENERTRLSTILKETENHFTKTTQDLNATKAQLQKAQVEFAQKDEGGKELQCKLVAEVELKERAQQELCQIKKQLSDLEANLCATRQELGRARCQNNQEEHRFHAREQELAQRLEEGRGREKRLEDQKHNLEVCLADATQQIQELKARLGGAEGRIRALDEQLSCVELHKRDTEQKLSSVVHTLRRIAGIQVDGSVNLSHRLLSPSRRFSPSRSCGDYDNRSTSQCPDGPIDVDPDLVRKGVRNLMHQVAQLEREKDDYKSQLGAAKKQLQDAADQQLRCDAKLGKLQAMLRNLQEEKSNLETDRKMKISAIQALEEKLKHRNDECQMLRERLAQTEMQLAATSEENGQNEERLEKSRQQCSKLDNEKRQLQEELAKVEGRASKLELQRVAMEGDLTRLQMALQEKDCSIRQMAERLENQNRALTQLEDRCTALKSTVDQLKERLQKSAVSETQLRGEIKTLQKELSEQGHCSQANEDKLKLVQKSLQTAENEKRILTERLDSAQTNLNELRRSQQAQLDGNQRLQEQVTDLEVQRSALESQLRIAKWNQESGGDKDLTNGNGGGNGEEELSRQLKSSQREKSELRSKLQTLQDKVKQLECDRKSKFSGGNAYDRAEKSNSFYGGAAESGEFDSNRYDVGGGNAGGGSFNCGLDHSVIEQETRDLRLKVRRLETLLAEKESELARCKARMNDSAKCHDGLDGDRYRSAQMHAEKLLDAREQSHRQQVLRLENQISMLREQLAQEAKRRQQYILRSSKANREMQHLRSTLGDSLRNVSQHPVDPHLLESESRRLDSAVSMSLPPSSCRDYDRD

Target ORF information:

RefSeq Version	NM_001300568.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rootletin, transcript variant E (Root), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300568.1

ORF Insert Sequence:

ATGCAGGCGT ATCGCGATAA CTTCAAGCAA ACGCCCTGCC CATCGGCCGT CGACCTTCAG 
GCGGCGGGAC CAGCCCACCA ATCGGCCACC GCCTCTCGGC TGCCCTTCTC GCGCAGCCAG 
CGTGGGCGTG ACCCCTCGGC AAGTGGCGCC TCAGTTTCCG CCTCCGTTGC TGGTGGTCTA 
ACGCCCAGGA TGATGAGTCC GGGTCCGCCG GGCGCCGGAG GAGGAGGAGG AGGAGGAGTG 
AGCGGCGGCG GCAGTGGAGA TCCCTCGGCC CTGTTGCGCC AGAATCAGGA GCTGCGCCAA 
CGGCTGGCCG ACGAGTCGCA TAGCTATCGA CGCCGCCTGG ACACCTACAA GCAGGCGCAG 
CACAACCAGG CCAACTTGGT CAGCCGGCTG CAGTCGAAGA TCCAGCAGTA TCGACAGCGG 
TGTAGCGACC TGGAGGAGCG CATGCACGAG ACCATTAAGC CGACGGCGGG AGTAGGACCC 
AAGCTGACCA CGGGTCCCAC CAACCAAGTG CTGTGTTCAA CTTCTTTAAC TCTGGGACAG 
AGCAGCTTGC CCTGCAGCTC CTCACTGGAC TCGCCGCCGC CCAGTTGCAG TCGTGACTAT 
GTTGACGATG TCTTGGTCAC CGGAGGCGGC GCCGGTGCGG CGGAACTGTG CCGCAAACTG 
GAGGAGGAGC ACCAGCGCTG CGAGCAGATT GTCGCCCAGA ACAGCGCACT GCGTCAGCAG 
CTGGAGGAGT CGAATCGCAC CAACGAGGCG CTCACCAACG ACCTACAGAA GCTGACCAAC 
GACTGGGCGA GTCTGCGGGA TGAGCTGCTG ATCAAGGAGG ATGAGTTCAA GGAGGAGGAG 
CAGGCCTTCA AGGACTACTA CAACAGCGAG CACAATCGCC TGCTGAAGAT GTGGCGCGAA 
GTTGTGGCCG TCAAGAGATC CTTCAAGGAG ATGCAGACGG CCATGAAGGC GGAGGTAGCC 
AAGATGGGTC AGGAGATCAA TTGTGTGGGC AAGGACATCA ATGGCTCCAA CGCAACGGTC 
GCCTTTGCCG TCCAGCAAGC CAAGCGGGCT GCGGATGATG AACTGAAGCA ATCGCAGCGC 
AGCAACGATG AACTCCAGAA TCAATTGGCC ACCCTGAAGG TGCAGTACGA GAGTGCCCGG 
CACGAGATCA TGGAGCGGGA TCAGCGACTA CTGGAGCTAA TGAATCAGTT GAAGAAGCTG 
GAGGATCGCT GCGCCCAAGC CGAATCCCAA GCAGCTCTGG CCAGTCGCTA TAGCGACGAG 
ATCGAGCGAC TGAACAATTC CATGCGAGAA ATCGCGCAGG CCGTCGTTCA AGATGCTGAG 
AACGCAGATC GCGAAGCAGA CGCCGAGGTC ACCGGCGGTG TCATGCAGCA CATGCACCTC 
ACGCGTGACG CCGCCTCTGT TGTGGGCGGA GCAGGTGGAG CGGGCAGCAC CGCCGGCGGC 
GGAGGGAAAT CACCGCGTCG CAACTCGACA CGCGCCTCTC AAGCCTTCGC CGAGGGCACC 
ATCTCAGCCG TCCAGGCGGC GCTCCACAAA TACCAGCTGG CCCTGCACGA CATGCAGGTG 
AAATTCCAGA ACACCAGCGA GACCCTGCGC ACCACCAAGG CCCAGCTGGA GACCAGCGAG 
GGTACCAAAC AGCTGCTGAC CACCAAGATG CAGCAGCTCA CCGAGAAACT GGACAGCAGC 
AACTCCAAGC TATCGGAATT GCTGCAGGAA AGGGAGAGTC TGCAGCGCGG ACTGGACGAT 
ATCCGTGTCC AGAAGCAGCA GTCTGAGATG GGACGAGCCG ATATCAATAG TGCGTTCGAG 
AATCTGAGCA GTGATTATGA GAAGATGCAG CTGAACTGTG GTAAACTCCA AAAACGTATC 
GATTCCATGG AGGAGGACAA AAAGGCAGTG GAGCTGGAGA TCCAACGTAT ACTGAAGGAC 
AAGAACATAA CCGAGTTGAA TTTGAGGTCT GAGGAAGATC GCAGTAGTCG TTTGCGGGAG 
GAAACCATAT CCTTGCGCGA GGAGCTTAAC CGAGTGAGCC TGAATCGTGA TCTTCTCGAG 
CAGCAGCGCA TCGAGTCCGA TAATTTGATC AATCTGCTCG AGAAACAGAA GTCCGACCTG 
GAGTACGATC TGGACAAGCT GTTGCTGGAG AAGTGCGATC TGCAGGAGAA GCACGAGAAG 
CTATCCAACA ATAGCTGCTC CACCAGTGAT GAGCTGAAGA GCGTTCAAAA TTGCCTTCAG 
GAGGCGCAGG AGGAGCGCAA GAAGCTCCGT ATTCAGTCCG TCGATCAGTG CAATGAAATC 
GGAGAGCTTA AGAAGGAGCT GGCGATACTG GACAAGGCAC GACTCGAACT GGAGACGGAC 
AATCTGTCGG CTGGGGAAAA GCTCAAGTGT CTGCAGCTGG AGAAGGAGAA AATTCTGCAA 
GACTTGGCCT GCGTCACCCG GGATCGTGGT GACATCCACA ATCAGCTAAC GGCGATGTGT 
CGCAAAAAGG AGGCTCTGAA TGAGGAACTT ATGCGGACTC GGCAGCGTCT GGAGCAAACC 
ACCGAGACCA ATAGCCGGCT GAATAGAAAT CTGGAGGAGA TGGTGAAGGA TGTGGAGGAG 
AAGCAAGTGG TCATCGATCT GCACGAGAAG GACACACATC GCTTGAACGA ACTCCTGGCC 
GCCCTGCGTT CGGAGAAGGA ATCCCTGGAA TCGGTGCTCT TCGATACAAA CACCTCACTG 
GAGGCCACCG AGGAGCGACG CAGTCAGCTG GAGCGGGATC TGCAGGAGGC TCTGGTGCGT 
GAGGAGAGCC TAAAGAATCA TGTGGCTCGC TTGCAAAAGG AGCTGGAGCA GTGTCAGCGC 
AAGGCCCAAG AGACCAAGAC GCAGCTGCTT AACGCCGCCC GTGCGGCTGA GAGTGACTTC 
AACCAGAAAA TCGCCAATCT GCAGGCTTGT GCAGAGGAGG CGGCCAAACG ACATGGCGAG 
GAGATTCTAC AGTTGCGAAA TGCCTTGGAG AAGCGAATGC AACAGGCTCT GCAAGCGTTG 
CAGACGGCCA AGGATGATGA GATCGAGAAG TTGCAGGAGC GTCTGGCCAC CTTGCAGGCG 
CATCTCGAGA GCCTTGTCCA GCAGCATGAG GAGGCACTGA TTCGGGCGGA GAGCGAGAAG 
CAGCAAGCCC TTTTGATTGC CCACCGGGAT AAGCAAGCGG TGGCCGAGCG TTTGGAGGCC 
GTATCCCGGG ATCTCAAGAC CGAACAGGAG TCCCTCGACC GGAGCAGACG GGAGGCCAAT 
GCGCGCGATG AGAAGCAGAG GGCTGCCATT GCCCAGCTGA AGGACGAGAT GGTGCAGATG 
CGCACCAAGG AGGAGGAGCA CAAGATTAAG TTGGAGGAAT GCATCCGGAA GCAGGAGCTG 
CAGTTGAGCA GCTTGCGCGA AGAACGCGAA TCCTTGTGCC GTGTGAGTGA GGAACTAAAG 
ATGGAGATTC GTCTGAAGGA GGACAGAATG GAGAGCACCA ACAATGAGTT GCAAGATGCG 
CTGCGCAAGT CCAAGGAGGG TGAGGGCTTC ATCGATAGCC TGCGCAAAGA GTTGACCGAC 
TGTCGCCGCC AACTGGCGGA CAGCAACATC GAGCGGGACA AGTATTCCGG CAGCAACAAG 
GAGCTGCGCG ACCACGTCAA GCGTGTGGAG AGCGCCAAGC GGGAGCAGGC GCGCGCCATC 
GAGGAGGCTC TGCAGAAGAT CAGCAATCTG GAGGATACCA AGAACTCGTT GGAGAACGAA 
CGCACTCGAT TGAGCACCAT ACTGAAGGAG ACGGAGAATC ACTTTACAAA GACCACCCAG 
GATCTGAATG CTACCAAGGC GCAGCTGCAG AAGGCTCAAG TGGAGTTCGC CCAGAAGGAC 
GAGGGCGGCA AGGAGTTGCA GTGCAAGCTG GTCGCCGAGG TGGAATTGAA GGAGCGGGCA 
CAGCAGGAGC TCTGCCAGAT TAAGAAGCAA TTATCGGATC TGGAAGCCAA TCTGTGTGCC 
ACTCGCCAGG AATTGGGCAG GGCTCGGTGT CAGAACAACC AGGAGGAGCA TCGCTTCCAT 
GCCAGGGAGC AGGAGTTGGC CCAGCGCTTG GAGGAGGGTC GTGGTAGGGA GAAGCGCCTG 
GAGGATCAGA AGCACAACCT GGAGGTCTGC CTAGCCGATG CCACTCAGCA GATCCAGGAG 
TTGAAGGCCC GTTTGGGCGG CGCCGAAGGT CGCATCCGTG CTTTGGATGA GCAGTTGTCC 
TGCGTGGAAC TACACAAGCG GGATACCGAA CAGAAGCTAT CCTCGGTGGT TCACACTCTG 
CGCCGGATTG CTGGCATCCA AGTGGACGGC AGTGTAAATT TGTCTCATCG CTTGCTGAGT 
CCCTCGCGAA GATTCAGTCC GTCTCGCAGC TGCGGAGACT ATGACAACAG GAGCACATCA 
CAATGCCCAG ATGGACCAAT TGATGTGGAC CCGGATCTGG TTAGGAAGGG TGTCCGCAAC 
CTGATGCATC AGGTGGCTCA GTTGGAGCGC GAGAAGGATG ACTACAAATC CCAATTGGGA 
GCAGCTAAGA AGCAACTCCA AGATGCTGCT GACCAGCAGC TACGATGCGA TGCCAAGCTG 
GGCAAACTGC AGGCCATGCT AAGAAATCTC CAAGAGGAGA AGAGCAATCT GGAAACGGAC 
CGCAAGATGA AAATCTCCGC CATCCAGGCG CTAGAGGAGA AGCTCAAGCA TCGCAACGAT 
GAGTGTCAGA TGCTAAGAGA ACGTTTGGCC CAAACAGAGA TGCAACTGGC TGCCACATCC 
GAGGAGAATG GCCAGAACGA GGAACGACTG GAGAAGAGCC GACAGCAGTG CTCCAAACTG 
GACAATGAGA AGCGCCAGCT GCAGGAGGAA TTGGCCAAGG TTGAGGGCCG GGCCAGCAAA 
CTCGAACTGC AGCGTGTTGC CATGGAGGGC GATCTTACCA GGCTACAAAT GGCCCTACAA 
GAGAAGGACT GCAGCATTCG TCAAATGGCT GAGCGGCTGG AGAACCAAAA CCGTGCCTTG 
ACCCAATTGG AGGATCGCTG CACCGCCCTT AAGTCCACCG TCGATCAGCT GAAGGAGCGC 
CTCCAGAAGT CCGCTGTGAG TGAAACCCAA CTGCGGGGCG AGATAAAGAC GCTCCAAAAG 
GAGCTTTCCG AGCAGGGTCA CTGCTCCCAG GCCAACGAGG ATAAGCTGAA GTTGGTGCAA 
AAGTCTCTGC AGACCGCTGA GAACGAGAAG CGCATCCTCA CCGAGCGCCT AGATAGCGCT 
CAGACCAATC TCAACGAGCT GCGACGTAGC CAACAGGCCC AGCTGGATGG TAACCAGCGC 
CTGCAGGAAC AGGTGACCGA TCTGGAGGTT CAGCGTTCGG CACTGGAGTC CCAACTTCGG 
ATTGCCAAGT GGAACCAGGA GAGTGGCGGC GACAAGGATC TGACAAACGG CAACGGAGGC 
GGAAATGGCG AGGAGGAGCT CAGCAGGCAG CTGAAGTCCT CGCAGCGGGA AAAGTCGGAG 
CTACGCAGCA AACTGCAAAC CCTACAGGAC AAAGTCAAAC AGTTGGAGTG CGACCGGAAA 
AGCAAGTTTT CGGGTGGAAA CGCCTATGAT CGGGCTGAGA AGTCCAACTC CTTTTACGGT 
GGCGCAGCTG AATCCGGCGA GTTCGACTCC AATCGCTACG ATGTGGGTGG AGGAAATGCT 
GGCGGAGGCT CTTTCAACTG CGGATTGGAT CACAGTGTAA TCGAGCAGGA GACGCGCGAT 
CTGCGACTCA AGGTGCGCCG CCTGGAGACA TTGTTGGCGG AGAAGGAGTC CGAGTTGGCG 
CGCTGCAAGG CGCGAATGAA CGACAGCGCC AAGTGCCATG ATGGCTTAGA TGGAGATCGT 
TATCGCAGTG CTCAGATGCA CGCAGAGAAG CTTCTTGACG CCAGGGAGCA GTCGCATCGG 
CAACAAGTGC TGCGCCTGGA GAACCAGATC TCAATGCTGC GCGAGCAGTT GGCCCAGGAG 
GCCAAACGGC GACAGCAGTA CATTCTACGC AGCTCGAAGG CCAATAGAGA AATGCAGCAT 
CTGAGGAGCA CCCTAGGAGA TTCCCTGCGC AATGTCTCCC AGCACCCGGT GGATCCCCAT 
CTCTTGGAAA GCGAGAGCCG GCGTCTCGAC TCGGCCGTTT CAATGAGCCT GCCACCGTCG 
AGCTGCCGGG ACTATGATCG CGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Root ( NM_142959.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651216.2 Drosophila melanogaster rootletin, transcript variant D (Root), mRNA.

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CloneID	OFa05895
Clone ID Related Accession (Same CDS sequence)	NM_001300568.1 , NM_142959.4 , NM_176553.2
Accession Version	NM_142959.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rootletin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_142959.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGCGT ATCGCGATAA CTTCAAGCAA ACGCCCTGCC CATCGGCCGT CGACCTTCAG 
GCGGCGGGAC CAGCCCACCA ATCGGCCACC GCCTCTCGGC TGCCCTTCTC GCGCAGCCAG 
CGTGGGCGTG ACCCCTCGGC AAGTGGCGCC TCAGTTTCCG CCTCCGTTGC TGGTGGTCTA 
ACGCCCAGGA TGATGAGTCC GGGTCCGCCG GGCGCCGGAG GAGGAGGAGG AGGAGGAGTG 
AGCGGCGGCG GCAGTGGAGA TCCCTCGGCC CTGTTGCGCC AGAATCAGGA GCTGCGCCAA 
CGGCTGGCCG ACGAGTCGCA TAGCTATCGA CGCCGCCTGG ACACCTACAA GCAGGCGCAG 
CACAACCAGG CCAACTTGGT CAGCCGGCTG CAGTCGAAGA TCCAGCAGTA TCGACAGCGG 
TGTAGCGACC TGGAGGAGCG CATGCACGAG ACCATTAAGC CGACGGCGGG AGTAGGACCC 
AAGCTGACCA CGGGTCCCAC CAACCAAGTG CTGTGTTCAA CTTCTTTAAC TCTGGGACAG 
AGCAGCTTGC CCTGCAGCTC CTCACTGGAC TCGCCGCCGC CCAGTTGCAG TCGTGACTAT 
GTTGACGATG TCTTGGTCAC CGGAGGCGGC GCCGGTGCGG CGGAACTGTG CCGCAAACTG 
GAGGAGGAGC ACCAGCGCTG CGAGCAGATT GTCGCCCAGA ACAGCGCACT GCGTCAGCAG 
CTGGAGGAGT CGAATCGCAC CAACGAGGCG CTCACCAACG ACCTACAGAA GCTGACCAAC 
GACTGGGCGA GTCTGCGGGA TGAGCTGCTG ATCAAGGAGG ATGAGTTCAA GGAGGAGGAG 
CAGGCCTTCA AGGACTACTA CAACAGCGAG CACAATCGCC TGCTGAAGAT GTGGCGCGAA 
GTTGTGGCCG TCAAGAGATC CTTCAAGGAG ATGCAGACGG CCATGAAGGC GGAGGTAGCC 
AAGATGGGTC AGGAGATCAA TTGTGTGGGC AAGGACATCA ATGGCTCCAA CGCAACGGTC 
GCCTTTGCCG TCCAGCAAGC CAAGCGGGCT GCGGATGATG AACTGAAGCA ATCGCAGCGC 
AGCAACGATG AACTCCAGAA TCAATTGGCC ACCCTGAAGG TGCAGTACGA GAGTGCCCGG 
CACGAGATCA TGGAGCGGGA TCAGCGACTA CTGGAGCTAA TGAATCAGTT GAAGAAGCTG 
GAGGATCGCT GCGCCCAAGC CGAATCCCAA GCAGCTCTGG CCAGTCGCTA TAGCGACGAG 
ATCGAGCGAC TGAACAATTC CATGCGAGAA ATCGCGCAGG CCGTCGTTCA AGATGCTGAG 
AACGCAGATC GCGAAGCAGA CGCCGAGGTC ACCGGCGGTG TCATGCAGCA CATGCACCTC 
ACGCGTGACG CCGCCTCTGT TGTGGGCGGA GCAGGTGGAG CGGGCAGCAC CGCCGGCGGC 
GGAGGGAAAT CACCGCGTCG CAACTCGACA CGCGCCTCTC AAGCCTTCGC CGAGGGCACC 
ATCTCAGCCG TCCAGGCGGC GCTCCACAAA TACCAGCTGG CCCTGCACGA CATGCAGGTG 
AAATTCCAGA ACACCAGCGA GACCCTGCGC ACCACCAAGG CCCAGCTGGA GACCAGCGAG 
GGTACCAAAC AGCTGCTGAC CACCAAGATG CAGCAGCTCA CCGAGAAACT GGACAGCAGC 
AACTCCAAGC TATCGGAATT GCTGCAGGAA AGGGAGAGTC TGCAGCGCGG ACTGGACGAT 
ATCCGTGTCC AGAAGCAGCA GTCTGAGATG GGACGAGCCG ATATCAATAG TGCGTTCGAG 
AATCTGAGCA GTGATTATGA GAAGATGCAG CTGAACTGTG GTAAACTCCA AAAACGTATC 
GATTCCATGG AGGAGGACAA AAAGGCAGTG GAGCTGGAGA TCCAACGTAT ACTGAAGGAC 
AAGAACATAA CCGAGTTGAA TTTGAGGTCT GAGGAAGATC GCAGTAGTCG TTTGCGGGAG 
GAAACCATAT CCTTGCGCGA GGAGCTTAAC CGAGTGAGCC TGAATCGTGA TCTTCTCGAG 
CAGCAGCGCA TCGAGTCCGA TAATTTGATC AATCTGCTCG AGAAACAGAA GTCCGACCTG 
GAGTACGATC TGGACAAGCT GTTGCTGGAG AAGTGCGATC TGCAGGAGAA GCACGAGAAG 
CTATCCAACA ATAGCTGCTC CACCAGTGAT GAGCTGAAGA GCGTTCAAAA TTGCCTTCAG 
GAGGCGCAGG AGGAGCGCAA GAAGCTCCGT ATTCAGTCCG TCGATCAGTG CAATGAAATC 
GGAGAGCTTA AGAAGGAGCT GGCGATACTG GACAAGGCAC GACTCGAACT GGAGACGGAC 
AATCTGTCGG CTGGGGAAAA GCTCAAGTGT CTGCAGCTGG AGAAGGAGAA AATTCTGCAA 
GACTTGGCCT GCGTCACCCG GGATCGTGGT GACATCCACA ATCAGCTAAC GGCGATGTGT 
CGCAAAAAGG AGGCTCTGAA TGAGGAACTT ATGCGGACTC GGCAGCGTCT GGAGCAAACC 
ACCGAGACCA ATAGCCGGCT GAATAGAAAT CTGGAGGAGA TGGTGAAGGA TGTGGAGGAG 
AAGCAAGTGG TCATCGATCT GCACGAGAAG GACACACATC GCTTGAACGA ACTCCTGGCC 
GCCCTGCGTT CGGAGAAGGA ATCCCTGGAA TCGGTGCTCT TCGATACAAA CACCTCACTG 
GAGGCCACCG AGGAGCGACG CAGTCAGCTG GAGCGGGATC TGCAGGAGGC TCTGGTGCGT 
GAGGAGAGCC TAAAGAATCA TGTGGCTCGC TTGCAAAAGG AGCTGGAGCA GTGTCAGCGC 
AAGGCCCAAG AGACCAAGAC GCAGCTGCTT AACGCCGCCC GTGCGGCTGA GAGTGACTTC 
AACCAGAAAA TCGCCAATCT GCAGGCTTGT GCAGAGGAGG CGGCCAAACG ACATGGCGAG 
GAGATTCTAC AGTTGCGAAA TGCCTTGGAG AAGCGAATGC AACAGGCTCT GCAAGCGTTG 
CAGACGGCCA AGGATGATGA GATCGAGAAG TTGCAGGAGC GTCTGGCCAC CTTGCAGGCG 
CATCTCGAGA GCCTTGTCCA GCAGCATGAG GAGGCACTGA TTCGGGCGGA GAGCGAGAAG 
CAGCAAGCCC TTTTGATTGC CCACCGGGAT AAGCAAGCGG TGGCCGAGCG TTTGGAGGCC 
GTATCCCGGG ATCTCAAGAC CGAACAGGAG TCCCTCGACC GGAGCAGACG GGAGGCCAAT 
GCGCGCGATG AGAAGCAGAG GGCTGCCATT GCCCAGCTGA AGGACGAGAT GGTGCAGATG 
CGCACCAAGG AGGAGGAGCA CAAGATTAAG TTGGAGGAAT GCATCCGGAA GCAGGAGCTG 
CAGTTGAGCA GCTTGCGCGA AGAACGCGAA TCCTTGTGCC GTGTGAGTGA GGAACTAAAG 
ATGGAGATTC GTCTGAAGGA GGACAGAATG GAGAGCACCA ACAATGAGTT GCAAGATGCG 
CTGCGCAAGT CCAAGGAGGG TGAGGGCTTC ATCGATAGCC TGCGCAAAGA GTTGACCGAC 
TGTCGCCGCC AACTGGCGGA CAGCAACATC GAGCGGGACA AGTATTCCGG CAGCAACAAG 
GAGCTGCGCG ACCACGTCAA GCGTGTGGAG AGCGCCAAGC GGGAGCAGGC GCGCGCCATC 
GAGGAGGCTC TGCAGAAGAT CAGCAATCTG GAGGATACCA AGAACTCGTT GGAGAACGAA 
CGCACTCGAT TGAGCACCAT ACTGAAGGAG ACGGAGAATC ACTTTACAAA GACCACCCAG 
GATCTGAATG CTACCAAGGC GCAGCTGCAG AAGGCTCAAG TGGAGTTCGC CCAGAAGGAC 
GAGGGCGGCA AGGAGTTGCA GTGCAAGCTG GTCGCCGAGG TGGAATTGAA GGAGCGGGCA 
CAGCAGGAGC TCTGCCAGAT TAAGAAGCAA TTATCGGATC TGGAAGCCAA TCTGTGTGCC 
ACTCGCCAGG AATTGGGCAG GGCTCGGTGT CAGAACAACC AGGAGGAGCA TCGCTTCCAT 
GCCAGGGAGC AGGAGTTGGC CCAGCGCTTG GAGGAGGGTC GTGGTAGGGA GAAGCGCCTG 
GAGGATCAGA AGCACAACCT GGAGGTCTGC CTAGCCGATG CCACTCAGCA GATCCAGGAG 
TTGAAGGCCC GTTTGGGCGG CGCCGAAGGT CGCATCCGTG CTTTGGATGA GCAGTTGTCC 
TGCGTGGAAC TACACAAGCG GGATACCGAA CAGAAGCTAT CCTCGGTGGT TCACACTCTG 
CGCCGGATTG CTGGCATCCA AGTGGACGGC AGTGTAAATT TGTCTCATCG CTTGCTGAGT 
CCCTCGCGAA GATTCAGTCC GTCTCGCAGC TGCGGAGACT ATGACAACAG GAGCACATCA 
CAATGCCCAG ATGGACCAAT TGATGTGGAC CCGGATCTGG TTAGGAAGGG TGTCCGCAAC 
CTGATGCATC AGGTGGCTCA GTTGGAGCGC GAGAAGGATG ACTACAAATC CCAATTGGGA 
GCAGCTAAGA AGCAACTCCA AGATGCTGCT GACCAGCAGC TACGATGCGA TGCCAAGCTG 
GGCAAACTGC AGGCCATGCT AAGAAATCTC CAAGAGGAGA AGAGCAATCT GGAAACGGAC 
CGCAAGATGA AAATCTCCGC CATCCAGGCG CTAGAGGAGA AGCTCAAGCA TCGCAACGAT 
GAGTGTCAGA TGCTAAGAGA ACGTTTGGCC CAAACAGAGA TGCAACTGGC TGCCACATCC 
GAGGAGAATG GCCAGAACGA GGAACGACTG GAGAAGAGCC GACAGCAGTG CTCCAAACTG 
GACAATGAGA AGCGCCAGCT GCAGGAGGAA TTGGCCAAGG TTGAGGGCCG GGCCAGCAAA 
CTCGAACTGC AGCGTGTTGC CATGGAGGGC GATCTTACCA GGCTACAAAT GGCCCTACAA 
GAGAAGGACT GCAGCATTCG TCAAATGGCT GAGCGGCTGG AGAACCAAAA CCGTGCCTTG 
ACCCAATTGG AGGATCGCTG CACCGCCCTT AAGTCCACCG TCGATCAGCT GAAGGAGCGC 
CTCCAGAAGT CCGCTGTGAG TGAAACCCAA CTGCGGGGCG AGATAAAGAC GCTCCAAAAG 
GAGCTTTCCG AGCAGGGTCA CTGCTCCCAG GCCAACGAGG ATAAGCTGAA GTTGGTGCAA 
AAGTCTCTGC AGACCGCTGA GAACGAGAAG CGCATCCTCA CCGAGCGCCT AGATAGCGCT 
CAGACCAATC TCAACGAGCT GCGACGTAGC CAACAGGCCC AGCTGGATGG TAACCAGCGC 
CTGCAGGAAC AGGTGACCGA TCTGGAGGTT CAGCGTTCGG CACTGGAGTC CCAACTTCGG 
ATTGCCAAGT GGAACCAGGA GAGTGGCGGC GACAAGGATC TGACAAACGG CAACGGAGGC 
GGAAATGGCG AGGAGGAGCT CAGCAGGCAG CTGAAGTCCT CGCAGCGGGA AAAGTCGGAG 
CTACGCAGCA AACTGCAAAC CCTACAGGAC AAAGTCAAAC AGTTGGAGTG CGACCGGAAA 
AGCAAGTTTT CGGGTGGAAA CGCCTATGAT CGGGCTGAGA AGTCCAACTC CTTTTACGGT 
GGCGCAGCTG AATCCGGCGA GTTCGACTCC AATCGCTACG ATGTGGGTGG AGGAAATGCT 
GGCGGAGGCT CTTTCAACTG CGGATTGGAT CACAGTGTAA TCGAGCAGGA GACGCGCGAT 
CTGCGACTCA AGGTGCGCCG CCTGGAGACA TTGTTGGCGG AGAAGGAGTC CGAGTTGGCG 
CGCTGCAAGG CGCGAATGAA CGACAGCGCC AAGTGCCATG ATGGCTTAGA TGGAGATCGT 
TATCGCAGTG CTCAGATGCA CGCAGAGAAG CTTCTTGACG CCAGGGAGCA GTCGCATCGG 
CAACAAGTGC TGCGCCTGGA GAACCAGATC TCAATGCTGC GCGAGCAGTT GGCCCAGGAG 
GCCAAACGGC GACAGCAGTA CATTCTACGC AGCTCGAAGG CCAATAGAGA AATGCAGCAT 
CTGAGGAGCA CCCTAGGAGA TTCCCTGCGC AATGTCTCCC AGCACCCGGT GGATCCCCAT 
CTCTTGGAAA GCGAGAGCCG GCGTCTCGAC TCGGCCGTTT CAATGAGCCT GCCACCGTCG 
AGCTGCCGGG ACTATGATCG CGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651216.2
CDS	95..6241
Translation	MQAYRDNFKQTPCPSAVDLQAAGPAHQSATASRLPFSRSQRGRDPSASGASVSASVAGGLTPRMMSPGPPGAGGGGGGGVSGGGSGDPSALLRQNQELRQRLADESHSYRRRLDTYKQAQHNQANLVSRLQSKIQQYRQRCSDLEERMHETIKPTAGVGPKLTTGPTNQVLCSTSLTLGQSSLPCSSSLDSPPPSCSRDYVDDVLVTGGGAGAAELCRKLEEEHQRCEQIVAQNSALRQQLEESNRTNEALTNDLQKLTNDWASLRDELLIKEDEFKEEEQAFKDYYNSEHNRLLKMWREVVAVKRSFKEMQTAMKAEVAKMGQEINCVGKDINGSNATVAFAVQQAKRAADDELKQSQRSNDELQNQLATLKVQYESARHEIMERDQRLLELMNQLKKLEDRCAQAESQAALASRYSDEIERLNNSMREIAQAVVQDAENADREADAEVTGGVMQHMHLTRDAASVVGGAGGAGSTAGGGGKSPRRNSTRASQAFAEGTISAVQAALHKYQLALHDMQVKFQNTSETLRTTKAQLETSEGTKQLLTTKMQQLTEKLDSSNSKLSELLQERESLQRGLDDIRVQKQQSEMGRADINSAFENLSSDYEKMQLNCGKLQKRIDSMEEDKKAVELEIQRILKDKNITELNLRSEEDRSSRLREETISLREELNRVSLNRDLLEQQRIESDNLINLLEKQKSDLEYDLDKLLLEKCDLQEKHEKLSNNSCSTSDELKSVQNCLQEAQEERKKLRIQSVDQCNEIGELKKELAILDKARLELETDNLSAGEKLKCLQLEKEKILQDLACVTRDRGDIHNQLTAMCRKKEALNEELMRTRQRLEQTTETNSRLNRNLEEMVKDVEEKQVVIDLHEKDTHRLNELLAALRSEKESLESVLFDTNTSLEATEERRSQLERDLQEALVREESLKNHVARLQKELEQCQRKAQETKTQLLNAARAAESDFNQKIANLQACAEEAAKRHGEEILQLRNALEKRMQQALQALQTAKDDEIEKLQERLATLQAHLESLVQQHEEALIRAESEKQQALLIAHRDKQAVAERLEAVSRDLKTEQESLDRSRREANARDEKQRAAIAQLKDEMVQMRTKEEEHKIKLEECIRKQELQLSSLREERESLCRVSEELKMEIRLKEDRMESTNNELQDALRKSKEGEGFIDSLRKELTDCRRQLADSNIERDKYSGSNKELRDHVKRVESAKREQARAIEEALQKISNLEDTKNSLENERTRLSTILKETENHFTKTTQDLNATKAQLQKAQVEFAQKDEGGKELQCKLVAEVELKERAQQELCQIKKQLSDLEANLCATRQELGRARCQNNQEEHRFHAREQELAQRLEEGRGREKRLEDQKHNLEVCLADATQQIQELKARLGGAEGRIRALDEQLSCVELHKRDTEQKLSSVVHTLRRIAGIQVDGSVNLSHRLLSPSRRFSPSRSCGDYDNRSTSQCPDGPIDVDPDLVRKGVRNLMHQVAQLEREKDDYKSQLGAAKKQLQDAADQQLRCDAKLGKLQAMLRNLQEEKSNLETDRKMKISAIQALEEKLKHRNDECQMLRERLAQTEMQLAATSEENGQNEERLEKSRQQCSKLDNEKRQLQEELAKVEGRASKLELQRVAMEGDLTRLQMALQEKDCSIRQMAERLENQNRALTQLEDRCTALKSTVDQLKERLQKSAVSETQLRGEIKTLQKELSEQGHCSQANEDKLKLVQKSLQTAENEKRILTERLDSAQTNLNELRRSQQAQLDGNQRLQEQVTDLEVQRSALESQLRIAKWNQESGGDKDLTNGNGGGNGEEELSRQLKSSQREKSELRSKLQTLQDKVKQLECDRKSKFSGGNAYDRAEKSNSFYGGAAESGEFDSNRYDVGGGNAGGGSFNCGLDHSVIEQETRDLRLKVRRLETLLAEKESELARCKARMNDSAKCHDGLDGDRYRSAQMHAEKLLDAREQSHRQQVLRLENQISMLREQLAQEAKRRQQYILRSSKANREMQHLRSTLGDSLRNVSQHPVDPHLLESESRRLDSAVSMSLPPSSCRDYDRD

Target ORF information:

RefSeq Version	NM_142959.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rootletin, transcript variant D (Root), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142959.4

ORF Insert Sequence:

ATGCAGGCGT ATCGCGATAA CTTCAAGCAA ACGCCCTGCC CATCGGCCGT CGACCTTCAG 
GCGGCGGGAC CAGCCCACCA ATCGGCCACC GCCTCTCGGC TGCCCTTCTC GCGCAGCCAG 
CGTGGGCGTG ACCCCTCGGC AAGTGGCGCC TCAGTTTCCG CCTCCGTTGC TGGTGGTCTA 
ACGCCCAGGA TGATGAGTCC GGGTCCGCCG GGCGCCGGAG GAGGAGGAGG AGGAGGAGTG 
AGCGGCGGCG GCAGTGGAGA TCCCTCGGCC CTGTTGCGCC AGAATCAGGA GCTGCGCCAA 
CGGCTGGCCG ACGAGTCGCA TAGCTATCGA CGCCGCCTGG ACACCTACAA GCAGGCGCAG 
CACAACCAGG CCAACTTGGT CAGCCGGCTG CAGTCGAAGA TCCAGCAGTA TCGACAGCGG 
TGTAGCGACC TGGAGGAGCG CATGCACGAG ACCATTAAGC CGACGGCGGG AGTAGGACCC 
AAGCTGACCA CGGGTCCCAC CAACCAAGTG CTGTGTTCAA CTTCTTTAAC TCTGGGACAG 
AGCAGCTTGC CCTGCAGCTC CTCACTGGAC TCGCCGCCGC CCAGTTGCAG TCGTGACTAT 
GTTGACGATG TCTTGGTCAC CGGAGGCGGC GCCGGTGCGG CGGAACTGTG CCGCAAACTG 
GAGGAGGAGC ACCAGCGCTG CGAGCAGATT GTCGCCCAGA ACAGCGCACT GCGTCAGCAG 
CTGGAGGAGT CGAATCGCAC CAACGAGGCG CTCACCAACG ACCTACAGAA GCTGACCAAC 
GACTGGGCGA GTCTGCGGGA TGAGCTGCTG ATCAAGGAGG ATGAGTTCAA GGAGGAGGAG 
CAGGCCTTCA AGGACTACTA CAACAGCGAG CACAATCGCC TGCTGAAGAT GTGGCGCGAA 
GTTGTGGCCG TCAAGAGATC CTTCAAGGAG ATGCAGACGG CCATGAAGGC GGAGGTAGCC 
AAGATGGGTC AGGAGATCAA TTGTGTGGGC AAGGACATCA ATGGCTCCAA CGCAACGGTC 
GCCTTTGCCG TCCAGCAAGC CAAGCGGGCT GCGGATGATG AACTGAAGCA ATCGCAGCGC 
AGCAACGATG AACTCCAGAA TCAATTGGCC ACCCTGAAGG TGCAGTACGA GAGTGCCCGG 
CACGAGATCA TGGAGCGGGA TCAGCGACTA CTGGAGCTAA TGAATCAGTT GAAGAAGCTG 
GAGGATCGCT GCGCCCAAGC CGAATCCCAA GCAGCTCTGG CCAGTCGCTA TAGCGACGAG 
ATCGAGCGAC TGAACAATTC CATGCGAGAA ATCGCGCAGG CCGTCGTTCA AGATGCTGAG 
AACGCAGATC GCGAAGCAGA CGCCGAGGTC ACCGGCGGTG TCATGCAGCA CATGCACCTC 
ACGCGTGACG CCGCCTCTGT TGTGGGCGGA GCAGGTGGAG CGGGCAGCAC CGCCGGCGGC 
GGAGGGAAAT CACCGCGTCG CAACTCGACA CGCGCCTCTC AAGCCTTCGC CGAGGGCACC 
ATCTCAGCCG TCCAGGCGGC GCTCCACAAA TACCAGCTGG CCCTGCACGA CATGCAGGTG 
AAATTCCAGA ACACCAGCGA GACCCTGCGC ACCACCAAGG CCCAGCTGGA GACCAGCGAG 
GGTACCAAAC AGCTGCTGAC CACCAAGATG CAGCAGCTCA CCGAGAAACT GGACAGCAGC 
AACTCCAAGC TATCGGAATT GCTGCAGGAA AGGGAGAGTC TGCAGCGCGG ACTGGACGAT 
ATCCGTGTCC AGAAGCAGCA GTCTGAGATG GGACGAGCCG ATATCAATAG TGCGTTCGAG 
AATCTGAGCA GTGATTATGA GAAGATGCAG CTGAACTGTG GTAAACTCCA AAAACGTATC 
GATTCCATGG AGGAGGACAA AAAGGCAGTG GAGCTGGAGA TCCAACGTAT ACTGAAGGAC 
AAGAACATAA CCGAGTTGAA TTTGAGGTCT GAGGAAGATC GCAGTAGTCG TTTGCGGGAG 
GAAACCATAT CCTTGCGCGA GGAGCTTAAC CGAGTGAGCC TGAATCGTGA TCTTCTCGAG 
CAGCAGCGCA TCGAGTCCGA TAATTTGATC AATCTGCTCG AGAAACAGAA GTCCGACCTG 
GAGTACGATC TGGACAAGCT GTTGCTGGAG AAGTGCGATC TGCAGGAGAA GCACGAGAAG 
CTATCCAACA ATAGCTGCTC CACCAGTGAT GAGCTGAAGA GCGTTCAAAA TTGCCTTCAG 
GAGGCGCAGG AGGAGCGCAA GAAGCTCCGT ATTCAGTCCG TCGATCAGTG CAATGAAATC 
GGAGAGCTTA AGAAGGAGCT GGCGATACTG GACAAGGCAC GACTCGAACT GGAGACGGAC 
AATCTGTCGG CTGGGGAAAA GCTCAAGTGT CTGCAGCTGG AGAAGGAGAA AATTCTGCAA 
GACTTGGCCT GCGTCACCCG GGATCGTGGT GACATCCACA ATCAGCTAAC GGCGATGTGT 
CGCAAAAAGG AGGCTCTGAA TGAGGAACTT ATGCGGACTC GGCAGCGTCT GGAGCAAACC 
ACCGAGACCA ATAGCCGGCT GAATAGAAAT CTGGAGGAGA TGGTGAAGGA TGTGGAGGAG 
AAGCAAGTGG TCATCGATCT GCACGAGAAG GACACACATC GCTTGAACGA ACTCCTGGCC 
GCCCTGCGTT CGGAGAAGGA ATCCCTGGAA TCGGTGCTCT TCGATACAAA CACCTCACTG 
GAGGCCACCG AGGAGCGACG CAGTCAGCTG GAGCGGGATC TGCAGGAGGC TCTGGTGCGT 
GAGGAGAGCC TAAAGAATCA TGTGGCTCGC TTGCAAAAGG AGCTGGAGCA GTGTCAGCGC 
AAGGCCCAAG AGACCAAGAC GCAGCTGCTT AACGCCGCCC GTGCGGCTGA GAGTGACTTC 
AACCAGAAAA TCGCCAATCT GCAGGCTTGT GCAGAGGAGG CGGCCAAACG ACATGGCGAG 
GAGATTCTAC AGTTGCGAAA TGCCTTGGAG AAGCGAATGC AACAGGCTCT GCAAGCGTTG 
CAGACGGCCA AGGATGATGA GATCGAGAAG TTGCAGGAGC GTCTGGCCAC CTTGCAGGCG 
CATCTCGAGA GCCTTGTCCA GCAGCATGAG GAGGCACTGA TTCGGGCGGA GAGCGAGAAG 
CAGCAAGCCC TTTTGATTGC CCACCGGGAT AAGCAAGCGG TGGCCGAGCG TTTGGAGGCC 
GTATCCCGGG ATCTCAAGAC CGAACAGGAG TCCCTCGACC GGAGCAGACG GGAGGCCAAT 
GCGCGCGATG AGAAGCAGAG GGCTGCCATT GCCCAGCTGA AGGACGAGAT GGTGCAGATG 
CGCACCAAGG AGGAGGAGCA CAAGATTAAG TTGGAGGAAT GCATCCGGAA GCAGGAGCTG 
CAGTTGAGCA GCTTGCGCGA AGAACGCGAA TCCTTGTGCC GTGTGAGTGA GGAACTAAAG 
ATGGAGATTC GTCTGAAGGA GGACAGAATG GAGAGCACCA ACAATGAGTT GCAAGATGCG 
CTGCGCAAGT CCAAGGAGGG TGAGGGCTTC ATCGATAGCC TGCGCAAAGA GTTGACCGAC 
TGTCGCCGCC AACTGGCGGA CAGCAACATC GAGCGGGACA AGTATTCCGG CAGCAACAAG 
GAGCTGCGCG ACCACGTCAA GCGTGTGGAG AGCGCCAAGC GGGAGCAGGC GCGCGCCATC 
GAGGAGGCTC TGCAGAAGAT CAGCAATCTG GAGGATACCA AGAACTCGTT GGAGAACGAA 
CGCACTCGAT TGAGCACCAT ACTGAAGGAG ACGGAGAATC ACTTTACAAA GACCACCCAG 
GATCTGAATG CTACCAAGGC GCAGCTGCAG AAGGCTCAAG TGGAGTTCGC CCAGAAGGAC 
GAGGGCGGCA AGGAGTTGCA GTGCAAGCTG GTCGCCGAGG TGGAATTGAA GGAGCGGGCA 
CAGCAGGAGC TCTGCCAGAT TAAGAAGCAA TTATCGGATC TGGAAGCCAA TCTGTGTGCC 
ACTCGCCAGG AATTGGGCAG GGCTCGGTGT CAGAACAACC AGGAGGAGCA TCGCTTCCAT 
GCCAGGGAGC AGGAGTTGGC CCAGCGCTTG GAGGAGGGTC GTGGTAGGGA GAAGCGCCTG 
GAGGATCAGA AGCACAACCT GGAGGTCTGC CTAGCCGATG CCACTCAGCA GATCCAGGAG 
TTGAAGGCCC GTTTGGGCGG CGCCGAAGGT CGCATCCGTG CTTTGGATGA GCAGTTGTCC 
TGCGTGGAAC TACACAAGCG GGATACCGAA CAGAAGCTAT CCTCGGTGGT TCACACTCTG 
CGCCGGATTG CTGGCATCCA AGTGGACGGC AGTGTAAATT TGTCTCATCG CTTGCTGAGT 
CCCTCGCGAA GATTCAGTCC GTCTCGCAGC TGCGGAGACT ATGACAACAG GAGCACATCA 
CAATGCCCAG ATGGACCAAT TGATGTGGAC CCGGATCTGG TTAGGAAGGG TGTCCGCAAC 
CTGATGCATC AGGTGGCTCA GTTGGAGCGC GAGAAGGATG ACTACAAATC CCAATTGGGA 
GCAGCTAAGA AGCAACTCCA AGATGCTGCT GACCAGCAGC TACGATGCGA TGCCAAGCTG 
GGCAAACTGC AGGCCATGCT AAGAAATCTC CAAGAGGAGA AGAGCAATCT GGAAACGGAC 
CGCAAGATGA AAATCTCCGC CATCCAGGCG CTAGAGGAGA AGCTCAAGCA TCGCAACGAT 
GAGTGTCAGA TGCTAAGAGA ACGTTTGGCC CAAACAGAGA TGCAACTGGC TGCCACATCC 
GAGGAGAATG GCCAGAACGA GGAACGACTG GAGAAGAGCC GACAGCAGTG CTCCAAACTG 
GACAATGAGA AGCGCCAGCT GCAGGAGGAA TTGGCCAAGG TTGAGGGCCG GGCCAGCAAA 
CTCGAACTGC AGCGTGTTGC CATGGAGGGC GATCTTACCA GGCTACAAAT GGCCCTACAA 
GAGAAGGACT GCAGCATTCG TCAAATGGCT GAGCGGCTGG AGAACCAAAA CCGTGCCTTG 
ACCCAATTGG AGGATCGCTG CACCGCCCTT AAGTCCACCG TCGATCAGCT GAAGGAGCGC 
CTCCAGAAGT CCGCTGTGAG TGAAACCCAA CTGCGGGGCG AGATAAAGAC GCTCCAAAAG 
GAGCTTTCCG AGCAGGGTCA CTGCTCCCAG GCCAACGAGG ATAAGCTGAA GTTGGTGCAA 
AAGTCTCTGC AGACCGCTGA GAACGAGAAG CGCATCCTCA CCGAGCGCCT AGATAGCGCT 
CAGACCAATC TCAACGAGCT GCGACGTAGC CAACAGGCCC AGCTGGATGG TAACCAGCGC 
CTGCAGGAAC AGGTGACCGA TCTGGAGGTT CAGCGTTCGG CACTGGAGTC CCAACTTCGG 
ATTGCCAAGT GGAACCAGGA GAGTGGCGGC GACAAGGATC TGACAAACGG CAACGGAGGC 
GGAAATGGCG AGGAGGAGCT CAGCAGGCAG CTGAAGTCCT CGCAGCGGGA AAAGTCGGAG 
CTACGCAGCA AACTGCAAAC CCTACAGGAC AAAGTCAAAC AGTTGGAGTG CGACCGGAAA 
AGCAAGTTTT CGGGTGGAAA CGCCTATGAT CGGGCTGAGA AGTCCAACTC CTTTTACGGT 
GGCGCAGCTG AATCCGGCGA GTTCGACTCC AATCGCTACG ATGTGGGTGG AGGAAATGCT 
GGCGGAGGCT CTTTCAACTG CGGATTGGAT CACAGTGTAA TCGAGCAGGA GACGCGCGAT 
CTGCGACTCA AGGTGCGCCG CCTGGAGACA TTGTTGGCGG AGAAGGAGTC CGAGTTGGCG 
CGCTGCAAGG CGCGAATGAA CGACAGCGCC AAGTGCCATG ATGGCTTAGA TGGAGATCGT 
TATCGCAGTG CTCAGATGCA CGCAGAGAAG CTTCTTGACG CCAGGGAGCA GTCGCATCGG 
CAACAAGTGC TGCGCCTGGA GAACCAGATC TCAATGCTGC GCGAGCAGTT GGCCCAGGAG 
GCCAAACGGC GACAGCAGTA CATTCTACGC AGCTCGAAGG CCAATAGAGA AATGCAGCAT 
CTGAGGAGCA CCCTAGGAGA TTCCCTGCGC AATGTCTCCC AGCACCCGGT GGATCCCCAT 
CTCTTGGAAA GCGAGAGCCG GCGTCTCGAC TCGGCCGTTT CAATGAGCCT GCCACCGTCG 
AGCTGCCGGG ACTATGATCG CGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Root ( NM_176553.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788730.2 Drosophila melanogaster rootletin, transcript variant F (Root), mRNA.

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CloneID	OFa05895
Clone ID Related Accession (Same CDS sequence)	NM_001300568.1 , NM_142959.4 , NM_176553.2
Accession Version	NM_176553.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rootletin, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_176553.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGCGT ATCGCGATAA CTTCAAGCAA ACGCCCTGCC CATCGGCCGT CGACCTTCAG 
GCGGCGGGAC CAGCCCACCA ATCGGCCACC GCCTCTCGGC TGCCCTTCTC GCGCAGCCAG 
CGTGGGCGTG ACCCCTCGGC AAGTGGCGCC TCAGTTTCCG CCTCCGTTGC TGGTGGTCTA 
ACGCCCAGGA TGATGAGTCC GGGTCCGCCG GGCGCCGGAG GAGGAGGAGG AGGAGGAGTG 
AGCGGCGGCG GCAGTGGAGA TCCCTCGGCC CTGTTGCGCC AGAATCAGGA GCTGCGCCAA 
CGGCTGGCCG ACGAGTCGCA TAGCTATCGA CGCCGCCTGG ACACCTACAA GCAGGCGCAG 
CACAACCAGG CCAACTTGGT CAGCCGGCTG CAGTCGAAGA TCCAGCAGTA TCGACAGCGG 
TGTAGCGACC TGGAGGAGCG CATGCACGAG ACCATTAAGC CGACGGCGGG AGTAGGACCC 
AAGCTGACCA CGGGTCCCAC CAACCAAGTG CTGTGTTCAA CTTCTTTAAC TCTGGGACAG 
AGCAGCTTGC CCTGCAGCTC CTCACTGGAC TCGCCGCCGC CCAGTTGCAG TCGTGACTAT 
GTTGACGATG TCTTGGTCAC CGGAGGCGGC GCCGGTGCGG CGGAACTGTG CCGCAAACTG 
GAGGAGGAGC ACCAGCGCTG CGAGCAGATT GTCGCCCAGA ACAGCGCACT GCGTCAGCAG 
CTGGAGGAGT CGAATCGCAC CAACGAGGCG CTCACCAACG ACCTACAGAA GCTGACCAAC 
GACTGGGCGA GTCTGCGGGA TGAGCTGCTG ATCAAGGAGG ATGAGTTCAA GGAGGAGGAG 
CAGGCCTTCA AGGACTACTA CAACAGCGAG CACAATCGCC TGCTGAAGAT GTGGCGCGAA 
GTTGTGGCCG TCAAGAGATC CTTCAAGGAG ATGCAGACGG CCATGAAGGC GGAGGTAGCC 
AAGATGGGTC AGGAGATCAA TTGTGTGGGC AAGGACATCA ATGGCTCCAA CGCAACGGTC 
GCCTTTGCCG TCCAGCAAGC CAAGCGGGCT GCGGATGATG AACTGAAGCA ATCGCAGCGC 
AGCAACGATG AACTCCAGAA TCAATTGGCC ACCCTGAAGG TGCAGTACGA GAGTGCCCGG 
CACGAGATCA TGGAGCGGGA TCAGCGACTA CTGGAGCTAA TGAATCAGTT GAAGAAGCTG 
GAGGATCGCT GCGCCCAAGC CGAATCCCAA GCAGCTCTGG CCAGTCGCTA TAGCGACGAG 
ATCGAGCGAC TGAACAATTC CATGCGAGAA ATCGCGCAGG CCGTCGTTCA AGATGCTGAG 
AACGCAGATC GCGAAGCAGA CGCCGAGGTC ACCGGCGGTG TCATGCAGCA CATGCACCTC 
ACGCGTGACG CCGCCTCTGT TGTGGGCGGA GCAGGTGGAG CGGGCAGCAC CGCCGGCGGC 
GGAGGGAAAT CACCGCGTCG CAACTCGACA CGCGCCTCTC AAGCCTTCGC CGAGGGCACC 
ATCTCAGCCG TCCAGGCGGC GCTCCACAAA TACCAGCTGG CCCTGCACGA CATGCAGGTG 
AAATTCCAGA ACACCAGCGA GACCCTGCGC ACCACCAAGG CCCAGCTGGA GACCAGCGAG 
GGTACCAAAC AGCTGCTGAC CACCAAGATG CAGCAGCTCA CCGAGAAACT GGACAGCAGC 
AACTCCAAGC TATCGGAATT GCTGCAGGAA AGGGAGAGTC TGCAGCGCGG ACTGGACGAT 
ATCCGTGTCC AGAAGCAGCA GTCTGAGATG GGACGAGCCG ATATCAATAG TGCGTTCGAG 
AATCTGAGCA GTGATTATGA GAAGATGCAG CTGAACTGTG GTAAACTCCA AAAACGTATC 
GATTCCATGG AGGAGGACAA AAAGGCAGTG GAGCTGGAGA TCCAACGTAT ACTGAAGGAC 
AAGAACATAA CCGAGTTGAA TTTGAGGTCT GAGGAAGATC GCAGTAGTCG TTTGCGGGAG 
GAAACCATAT CCTTGCGCGA GGAGCTTAAC CGAGTGAGCC TGAATCGTGA TCTTCTCGAG 
CAGCAGCGCA TCGAGTCCGA TAATTTGATC AATCTGCTCG AGAAACAGAA GTCCGACCTG 
GAGTACGATC TGGACAAGCT GTTGCTGGAG AAGTGCGATC TGCAGGAGAA GCACGAGAAG 
CTATCCAACA ATAGCTGCTC CACCAGTGAT GAGCTGAAGA GCGTTCAAAA TTGCCTTCAG 
GAGGCGCAGG AGGAGCGCAA GAAGCTCCGT ATTCAGTCCG TCGATCAGTG CAATGAAATC 
GGAGAGCTTA AGAAGGAGCT GGCGATACTG GACAAGGCAC GACTCGAACT GGAGACGGAC 
AATCTGTCGG CTGGGGAAAA GCTCAAGTGT CTGCAGCTGG AGAAGGAGAA AATTCTGCAA 
GACTTGGCCT GCGTCACCCG GGATCGTGGT GACATCCACA ATCAGCTAAC GGCGATGTGT 
CGCAAAAAGG AGGCTCTGAA TGAGGAACTT ATGCGGACTC GGCAGCGTCT GGAGCAAACC 
ACCGAGACCA ATAGCCGGCT GAATAGAAAT CTGGAGGAGA TGGTGAAGGA TGTGGAGGAG 
AAGCAAGTGG TCATCGATCT GCACGAGAAG GACACACATC GCTTGAACGA ACTCCTGGCC 
GCCCTGCGTT CGGAGAAGGA ATCCCTGGAA TCGGTGCTCT TCGATACAAA CACCTCACTG 
GAGGCCACCG AGGAGCGACG CAGTCAGCTG GAGCGGGATC TGCAGGAGGC TCTGGTGCGT 
GAGGAGAGCC TAAAGAATCA TGTGGCTCGC TTGCAAAAGG AGCTGGAGCA GTGTCAGCGC 
AAGGCCCAAG AGACCAAGAC GCAGCTGCTT AACGCCGCCC GTGCGGCTGA GAGTGACTTC 
AACCAGAAAA TCGCCAATCT GCAGGCTTGT GCAGAGGAGG CGGCCAAACG ACATGGCGAG 
GAGATTCTAC AGTTGCGAAA TGCCTTGGAG AAGCGAATGC AACAGGCTCT GCAAGCGTTG 
CAGACGGCCA AGGATGATGA GATCGAGAAG TTGCAGGAGC GTCTGGCCAC CTTGCAGGCG 
CATCTCGAGA GCCTTGTCCA GCAGCATGAG GAGGCACTGA TTCGGGCGGA GAGCGAGAAG 
CAGCAAGCCC TTTTGATTGC CCACCGGGAT AAGCAAGCGG TGGCCGAGCG TTTGGAGGCC 
GTATCCCGGG ATCTCAAGAC CGAACAGGAG TCCCTCGACC GGAGCAGACG GGAGGCCAAT 
GCGCGCGATG AGAAGCAGAG GGCTGCCATT GCCCAGCTGA AGGACGAGAT GGTGCAGATG 
CGCACCAAGG AGGAGGAGCA CAAGATTAAG TTGGAGGAAT GCATCCGGAA GCAGGAGCTG 
CAGTTGAGCA GCTTGCGCGA AGAACGCGAA TCCTTGTGCC GTGTGAGTGA GGAACTAAAG 
ATGGAGATTC GTCTGAAGGA GGACAGAATG GAGAGCACCA ACAATGAGTT GCAAGATGCG 
CTGCGCAAGT CCAAGGAGGG TGAGGGCTTC ATCGATAGCC TGCGCAAAGA GTTGACCGAC 
TGTCGCCGCC AACTGGCGGA CAGCAACATC GAGCGGGACA AGTATTCCGG CAGCAACAAG 
GAGCTGCGCG ACCACGTCAA GCGTGTGGAG AGCGCCAAGC GGGAGCAGGC GCGCGCCATC 
GAGGAGGCTC TGCAGAAGAT CAGCAATCTG GAGGATACCA AGAACTCGTT GGAGAACGAA 
CGCACTCGAT TGAGCACCAT ACTGAAGGAG ACGGAGAATC ACTTTACAAA GACCACCCAG 
GATCTGAATG CTACCAAGGC GCAGCTGCAG AAGGCTCAAG TGGAGTTCGC CCAGAAGGAC 
GAGGGCGGCA AGGAGTTGCA GTGCAAGCTG GTCGCCGAGG TGGAATTGAA GGAGCGGGCA 
CAGCAGGAGC TCTGCCAGAT TAAGAAGCAA TTATCGGATC TGGAAGCCAA TCTGTGTGCC 
ACTCGCCAGG AATTGGGCAG GGCTCGGTGT CAGAACAACC AGGAGGAGCA TCGCTTCCAT 
GCCAGGGAGC AGGAGTTGGC CCAGCGCTTG GAGGAGGGTC GTGGTAGGGA GAAGCGCCTG 
GAGGATCAGA AGCACAACCT GGAGGTCTGC CTAGCCGATG CCACTCAGCA GATCCAGGAG 
TTGAAGGCCC GTTTGGGCGG CGCCGAAGGT CGCATCCGTG CTTTGGATGA GCAGTTGTCC 
TGCGTGGAAC TACACAAGCG GGATACCGAA CAGAAGCTAT CCTCGGTGGT TCACACTCTG 
CGCCGGATTG CTGGCATCCA AGTGGACGGC AGTGTAAATT TGTCTCATCG CTTGCTGAGT 
CCCTCGCGAA GATTCAGTCC GTCTCGCAGC TGCGGAGACT ATGACAACAG GAGCACATCA 
CAATGCCCAG ATGGACCAAT TGATGTGGAC CCGGATCTGG TTAGGAAGGG TGTCCGCAAC 
CTGATGCATC AGGTGGCTCA GTTGGAGCGC GAGAAGGATG ACTACAAATC CCAATTGGGA 
GCAGCTAAGA AGCAACTCCA AGATGCTGCT GACCAGCAGC TACGATGCGA TGCCAAGCTG 
GGCAAACTGC AGGCCATGCT AAGAAATCTC CAAGAGGAGA AGAGCAATCT GGAAACGGAC 
CGCAAGATGA AAATCTCCGC CATCCAGGCG CTAGAGGAGA AGCTCAAGCA TCGCAACGAT 
GAGTGTCAGA TGCTAAGAGA ACGTTTGGCC CAAACAGAGA TGCAACTGGC TGCCACATCC 
GAGGAGAATG GCCAGAACGA GGAACGACTG GAGAAGAGCC GACAGCAGTG CTCCAAACTG 
GACAATGAGA AGCGCCAGCT GCAGGAGGAA TTGGCCAAGG TTGAGGGCCG GGCCAGCAAA 
CTCGAACTGC AGCGTGTTGC CATGGAGGGC GATCTTACCA GGCTACAAAT GGCCCTACAA 
GAGAAGGACT GCAGCATTCG TCAAATGGCT GAGCGGCTGG AGAACCAAAA CCGTGCCTTG 
ACCCAATTGG AGGATCGCTG CACCGCCCTT AAGTCCACCG TCGATCAGCT GAAGGAGCGC 
CTCCAGAAGT CCGCTGTGAG TGAAACCCAA CTGCGGGGCG AGATAAAGAC GCTCCAAAAG 
GAGCTTTCCG AGCAGGGTCA CTGCTCCCAG GCCAACGAGG ATAAGCTGAA GTTGGTGCAA 
AAGTCTCTGC AGACCGCTGA GAACGAGAAG CGCATCCTCA CCGAGCGCCT AGATAGCGCT 
CAGACCAATC TCAACGAGCT GCGACGTAGC CAACAGGCCC AGCTGGATGG TAACCAGCGC 
CTGCAGGAAC AGGTGACCGA TCTGGAGGTT CAGCGTTCGG CACTGGAGTC CCAACTTCGG 
ATTGCCAAGT GGAACCAGGA GAGTGGCGGC GACAAGGATC TGACAAACGG CAACGGAGGC 
GGAAATGGCG AGGAGGAGCT CAGCAGGCAG CTGAAGTCCT CGCAGCGGGA AAAGTCGGAG 
CTACGCAGCA AACTGCAAAC CCTACAGGAC AAAGTCAAAC AGTTGGAGTG CGACCGGAAA 
AGCAAGTTTT CGGGTGGAAA CGCCTATGAT CGGGCTGAGA AGTCCAACTC CTTTTACGGT 
GGCGCAGCTG AATCCGGCGA GTTCGACTCC AATCGCTACG ATGTGGGTGG AGGAAATGCT 
GGCGGAGGCT CTTTCAACTG CGGATTGGAT CACAGTGTAA TCGAGCAGGA GACGCGCGAT 
CTGCGACTCA AGGTGCGCCG CCTGGAGACA TTGTTGGCGG AGAAGGAGTC CGAGTTGGCG 
CGCTGCAAGG CGCGAATGAA CGACAGCGCC AAGTGCCATG ATGGCTTAGA TGGAGATCGT 
TATCGCAGTG CTCAGATGCA CGCAGAGAAG CTTCTTGACG CCAGGGAGCA GTCGCATCGG 
CAACAAGTGC TGCGCCTGGA GAACCAGATC TCAATGCTGC GCGAGCAGTT GGCCCAGGAG 
GCCAAACGGC GACAGCAGTA CATTCTACGC AGCTCGAAGG CCAATAGAGA AATGCAGCAT 
CTGAGGAGCA CCCTAGGAGA TTCCCTGCGC AATGTCTCCC AGCACCCGGT GGATCCCCAT 
CTCTTGGAAA GCGAGAGCCG GCGTCTCGAC TCGGCCGTTT CAATGAGCCT GCCACCGTCG 
AGCTGCCGGG ACTATGATCG CGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788730.2
CDS	369..6515
Translation	MQAYRDNFKQTPCPSAVDLQAAGPAHQSATASRLPFSRSQRGRDPSASGASVSASVAGGLTPRMMSPGPPGAGGGGGGGVSGGGSGDPSALLRQNQELRQRLADESHSYRRRLDTYKQAQHNQANLVSRLQSKIQQYRQRCSDLEERMHETIKPTAGVGPKLTTGPTNQVLCSTSLTLGQSSLPCSSSLDSPPPSCSRDYVDDVLVTGGGAGAAELCRKLEEEHQRCEQIVAQNSALRQQLEESNRTNEALTNDLQKLTNDWASLRDELLIKEDEFKEEEQAFKDYYNSEHNRLLKMWREVVAVKRSFKEMQTAMKAEVAKMGQEINCVGKDINGSNATVAFAVQQAKRAADDELKQSQRSNDELQNQLATLKVQYESARHEIMERDQRLLELMNQLKKLEDRCAQAESQAALASRYSDEIERLNNSMREIAQAVVQDAENADREADAEVTGGVMQHMHLTRDAASVVGGAGGAGSTAGGGGKSPRRNSTRASQAFAEGTISAVQAALHKYQLALHDMQVKFQNTSETLRTTKAQLETSEGTKQLLTTKMQQLTEKLDSSNSKLSELLQERESLQRGLDDIRVQKQQSEMGRADINSAFENLSSDYEKMQLNCGKLQKRIDSMEEDKKAVELEIQRILKDKNITELNLRSEEDRSSRLREETISLREELNRVSLNRDLLEQQRIESDNLINLLEKQKSDLEYDLDKLLLEKCDLQEKHEKLSNNSCSTSDELKSVQNCLQEAQEERKKLRIQSVDQCNEIGELKKELAILDKARLELETDNLSAGEKLKCLQLEKEKILQDLACVTRDRGDIHNQLTAMCRKKEALNEELMRTRQRLEQTTETNSRLNRNLEEMVKDVEEKQVVIDLHEKDTHRLNELLAALRSEKESLESVLFDTNTSLEATEERRSQLERDLQEALVREESLKNHVARLQKELEQCQRKAQETKTQLLNAARAAESDFNQKIANLQACAEEAAKRHGEEILQLRNALEKRMQQALQALQTAKDDEIEKLQERLATLQAHLESLVQQHEEALIRAESEKQQALLIAHRDKQAVAERLEAVSRDLKTEQESLDRSRREANARDEKQRAAIAQLKDEMVQMRTKEEEHKIKLEECIRKQELQLSSLREERESLCRVSEELKMEIRLKEDRMESTNNELQDALRKSKEGEGFIDSLRKELTDCRRQLADSNIERDKYSGSNKELRDHVKRVESAKREQARAIEEALQKISNLEDTKNSLENERTRLSTILKETENHFTKTTQDLNATKAQLQKAQVEFAQKDEGGKELQCKLVAEVELKERAQQELCQIKKQLSDLEANLCATRQELGRARCQNNQEEHRFHAREQELAQRLEEGRGREKRLEDQKHNLEVCLADATQQIQELKARLGGAEGRIRALDEQLSCVELHKRDTEQKLSSVVHTLRRIAGIQVDGSVNLSHRLLSPSRRFSPSRSCGDYDNRSTSQCPDGPIDVDPDLVRKGVRNLMHQVAQLEREKDDYKSQLGAAKKQLQDAADQQLRCDAKLGKLQAMLRNLQEEKSNLETDRKMKISAIQALEEKLKHRNDECQMLRERLAQTEMQLAATSEENGQNEERLEKSRQQCSKLDNEKRQLQEELAKVEGRASKLELQRVAMEGDLTRLQMALQEKDCSIRQMAERLENQNRALTQLEDRCTALKSTVDQLKERLQKSAVSETQLRGEIKTLQKELSEQGHCSQANEDKLKLVQKSLQTAENEKRILTERLDSAQTNLNELRRSQQAQLDGNQRLQEQVTDLEVQRSALESQLRIAKWNQESGGDKDLTNGNGGGNGEEELSRQLKSSQREKSELRSKLQTLQDKVKQLECDRKSKFSGGNAYDRAEKSNSFYGGAAESGEFDSNRYDVGGGNAGGGSFNCGLDHSVIEQETRDLRLKVRRLETLLAEKESELARCKARMNDSAKCHDGLDGDRYRSAQMHAEKLLDAREQSHRQQVLRLENQISMLREQLAQEAKRRQQYILRSSKANREMQHLRSTLGDSLRNVSQHPVDPHLLESESRRLDSAVSMSLPPSSCRDYDRD

Target ORF information:

RefSeq Version	NM_176553.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rootletin, transcript variant F (Root), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176553.2

ORF Insert Sequence:

ATGCAGGCGT ATCGCGATAA CTTCAAGCAA ACGCCCTGCC CATCGGCCGT CGACCTTCAG 
GCGGCGGGAC CAGCCCACCA ATCGGCCACC GCCTCTCGGC TGCCCTTCTC GCGCAGCCAG 
CGTGGGCGTG ACCCCTCGGC AAGTGGCGCC TCAGTTTCCG CCTCCGTTGC TGGTGGTCTA 
ACGCCCAGGA TGATGAGTCC GGGTCCGCCG GGCGCCGGAG GAGGAGGAGG AGGAGGAGTG 
AGCGGCGGCG GCAGTGGAGA TCCCTCGGCC CTGTTGCGCC AGAATCAGGA GCTGCGCCAA 
CGGCTGGCCG ACGAGTCGCA TAGCTATCGA CGCCGCCTGG ACACCTACAA GCAGGCGCAG 
CACAACCAGG CCAACTTGGT CAGCCGGCTG CAGTCGAAGA TCCAGCAGTA TCGACAGCGG 
TGTAGCGACC TGGAGGAGCG CATGCACGAG ACCATTAAGC CGACGGCGGG AGTAGGACCC 
AAGCTGACCA CGGGTCCCAC CAACCAAGTG CTGTGTTCAA CTTCTTTAAC TCTGGGACAG 
AGCAGCTTGC CCTGCAGCTC CTCACTGGAC TCGCCGCCGC CCAGTTGCAG TCGTGACTAT 
GTTGACGATG TCTTGGTCAC CGGAGGCGGC GCCGGTGCGG CGGAACTGTG CCGCAAACTG 
GAGGAGGAGC ACCAGCGCTG CGAGCAGATT GTCGCCCAGA ACAGCGCACT GCGTCAGCAG 
CTGGAGGAGT CGAATCGCAC CAACGAGGCG CTCACCAACG ACCTACAGAA GCTGACCAAC 
GACTGGGCGA GTCTGCGGGA TGAGCTGCTG ATCAAGGAGG ATGAGTTCAA GGAGGAGGAG 
CAGGCCTTCA AGGACTACTA CAACAGCGAG CACAATCGCC TGCTGAAGAT GTGGCGCGAA 
GTTGTGGCCG TCAAGAGATC CTTCAAGGAG ATGCAGACGG CCATGAAGGC GGAGGTAGCC 
AAGATGGGTC AGGAGATCAA TTGTGTGGGC AAGGACATCA ATGGCTCCAA CGCAACGGTC 
GCCTTTGCCG TCCAGCAAGC CAAGCGGGCT GCGGATGATG AACTGAAGCA ATCGCAGCGC 
AGCAACGATG AACTCCAGAA TCAATTGGCC ACCCTGAAGG TGCAGTACGA GAGTGCCCGG 
CACGAGATCA TGGAGCGGGA TCAGCGACTA CTGGAGCTAA TGAATCAGTT GAAGAAGCTG 
GAGGATCGCT GCGCCCAAGC CGAATCCCAA GCAGCTCTGG CCAGTCGCTA TAGCGACGAG 
ATCGAGCGAC TGAACAATTC CATGCGAGAA ATCGCGCAGG CCGTCGTTCA AGATGCTGAG 
AACGCAGATC GCGAAGCAGA CGCCGAGGTC ACCGGCGGTG TCATGCAGCA CATGCACCTC 
ACGCGTGACG CCGCCTCTGT TGTGGGCGGA GCAGGTGGAG CGGGCAGCAC CGCCGGCGGC 
GGAGGGAAAT CACCGCGTCG CAACTCGACA CGCGCCTCTC AAGCCTTCGC CGAGGGCACC 
ATCTCAGCCG TCCAGGCGGC GCTCCACAAA TACCAGCTGG CCCTGCACGA CATGCAGGTG 
AAATTCCAGA ACACCAGCGA GACCCTGCGC ACCACCAAGG CCCAGCTGGA GACCAGCGAG 
GGTACCAAAC AGCTGCTGAC CACCAAGATG CAGCAGCTCA CCGAGAAACT GGACAGCAGC 
AACTCCAAGC TATCGGAATT GCTGCAGGAA AGGGAGAGTC TGCAGCGCGG ACTGGACGAT 
ATCCGTGTCC AGAAGCAGCA GTCTGAGATG GGACGAGCCG ATATCAATAG TGCGTTCGAG 
AATCTGAGCA GTGATTATGA GAAGATGCAG CTGAACTGTG GTAAACTCCA AAAACGTATC 
GATTCCATGG AGGAGGACAA AAAGGCAGTG GAGCTGGAGA TCCAACGTAT ACTGAAGGAC 
AAGAACATAA CCGAGTTGAA TTTGAGGTCT GAGGAAGATC GCAGTAGTCG TTTGCGGGAG 
GAAACCATAT CCTTGCGCGA GGAGCTTAAC CGAGTGAGCC TGAATCGTGA TCTTCTCGAG 
CAGCAGCGCA TCGAGTCCGA TAATTTGATC AATCTGCTCG AGAAACAGAA GTCCGACCTG 
GAGTACGATC TGGACAAGCT GTTGCTGGAG AAGTGCGATC TGCAGGAGAA GCACGAGAAG 
CTATCCAACA ATAGCTGCTC CACCAGTGAT GAGCTGAAGA GCGTTCAAAA TTGCCTTCAG 
GAGGCGCAGG AGGAGCGCAA GAAGCTCCGT ATTCAGTCCG TCGATCAGTG CAATGAAATC 
GGAGAGCTTA AGAAGGAGCT GGCGATACTG GACAAGGCAC GACTCGAACT GGAGACGGAC 
AATCTGTCGG CTGGGGAAAA GCTCAAGTGT CTGCAGCTGG AGAAGGAGAA AATTCTGCAA 
GACTTGGCCT GCGTCACCCG GGATCGTGGT GACATCCACA ATCAGCTAAC GGCGATGTGT 
CGCAAAAAGG AGGCTCTGAA TGAGGAACTT ATGCGGACTC GGCAGCGTCT GGAGCAAACC 
ACCGAGACCA ATAGCCGGCT GAATAGAAAT CTGGAGGAGA TGGTGAAGGA TGTGGAGGAG 
AAGCAAGTGG TCATCGATCT GCACGAGAAG GACACACATC GCTTGAACGA ACTCCTGGCC 
GCCCTGCGTT CGGAGAAGGA ATCCCTGGAA TCGGTGCTCT TCGATACAAA CACCTCACTG 
GAGGCCACCG AGGAGCGACG CAGTCAGCTG GAGCGGGATC TGCAGGAGGC TCTGGTGCGT 
GAGGAGAGCC TAAAGAATCA TGTGGCTCGC TTGCAAAAGG AGCTGGAGCA GTGTCAGCGC 
AAGGCCCAAG AGACCAAGAC GCAGCTGCTT AACGCCGCCC GTGCGGCTGA GAGTGACTTC 
AACCAGAAAA TCGCCAATCT GCAGGCTTGT GCAGAGGAGG CGGCCAAACG ACATGGCGAG 
GAGATTCTAC AGTTGCGAAA TGCCTTGGAG AAGCGAATGC AACAGGCTCT GCAAGCGTTG 
CAGACGGCCA AGGATGATGA GATCGAGAAG TTGCAGGAGC GTCTGGCCAC CTTGCAGGCG 
CATCTCGAGA GCCTTGTCCA GCAGCATGAG GAGGCACTGA TTCGGGCGGA GAGCGAGAAG 
CAGCAAGCCC TTTTGATTGC CCACCGGGAT AAGCAAGCGG TGGCCGAGCG TTTGGAGGCC 
GTATCCCGGG ATCTCAAGAC CGAACAGGAG TCCCTCGACC GGAGCAGACG GGAGGCCAAT 
GCGCGCGATG AGAAGCAGAG GGCTGCCATT GCCCAGCTGA AGGACGAGAT GGTGCAGATG 
CGCACCAAGG AGGAGGAGCA CAAGATTAAG TTGGAGGAAT GCATCCGGAA GCAGGAGCTG 
CAGTTGAGCA GCTTGCGCGA AGAACGCGAA TCCTTGTGCC GTGTGAGTGA GGAACTAAAG 
ATGGAGATTC GTCTGAAGGA GGACAGAATG GAGAGCACCA ACAATGAGTT GCAAGATGCG 
CTGCGCAAGT CCAAGGAGGG TGAGGGCTTC ATCGATAGCC TGCGCAAAGA GTTGACCGAC 
TGTCGCCGCC AACTGGCGGA CAGCAACATC GAGCGGGACA AGTATTCCGG CAGCAACAAG 
GAGCTGCGCG ACCACGTCAA GCGTGTGGAG AGCGCCAAGC GGGAGCAGGC GCGCGCCATC 
GAGGAGGCTC TGCAGAAGAT CAGCAATCTG GAGGATACCA AGAACTCGTT GGAGAACGAA 
CGCACTCGAT TGAGCACCAT ACTGAAGGAG ACGGAGAATC ACTTTACAAA GACCACCCAG 
GATCTGAATG CTACCAAGGC GCAGCTGCAG AAGGCTCAAG TGGAGTTCGC CCAGAAGGAC 
GAGGGCGGCA AGGAGTTGCA GTGCAAGCTG GTCGCCGAGG TGGAATTGAA GGAGCGGGCA 
CAGCAGGAGC TCTGCCAGAT TAAGAAGCAA TTATCGGATC TGGAAGCCAA TCTGTGTGCC 
ACTCGCCAGG AATTGGGCAG GGCTCGGTGT CAGAACAACC AGGAGGAGCA TCGCTTCCAT 
GCCAGGGAGC AGGAGTTGGC CCAGCGCTTG GAGGAGGGTC GTGGTAGGGA GAAGCGCCTG 
GAGGATCAGA AGCACAACCT GGAGGTCTGC CTAGCCGATG CCACTCAGCA GATCCAGGAG 
TTGAAGGCCC GTTTGGGCGG CGCCGAAGGT CGCATCCGTG CTTTGGATGA GCAGTTGTCC 
TGCGTGGAAC TACACAAGCG GGATACCGAA CAGAAGCTAT CCTCGGTGGT TCACACTCTG 
CGCCGGATTG CTGGCATCCA AGTGGACGGC AGTGTAAATT TGTCTCATCG CTTGCTGAGT 
CCCTCGCGAA GATTCAGTCC GTCTCGCAGC TGCGGAGACT ATGACAACAG GAGCACATCA 
CAATGCCCAG ATGGACCAAT TGATGTGGAC CCGGATCTGG TTAGGAAGGG TGTCCGCAAC 
CTGATGCATC AGGTGGCTCA GTTGGAGCGC GAGAAGGATG ACTACAAATC CCAATTGGGA 
GCAGCTAAGA AGCAACTCCA AGATGCTGCT GACCAGCAGC TACGATGCGA TGCCAAGCTG 
GGCAAACTGC AGGCCATGCT AAGAAATCTC CAAGAGGAGA AGAGCAATCT GGAAACGGAC 
CGCAAGATGA AAATCTCCGC CATCCAGGCG CTAGAGGAGA AGCTCAAGCA TCGCAACGAT 
GAGTGTCAGA TGCTAAGAGA ACGTTTGGCC CAAACAGAGA TGCAACTGGC TGCCACATCC 
GAGGAGAATG GCCAGAACGA GGAACGACTG GAGAAGAGCC GACAGCAGTG CTCCAAACTG 
GACAATGAGA AGCGCCAGCT GCAGGAGGAA TTGGCCAAGG TTGAGGGCCG GGCCAGCAAA 
CTCGAACTGC AGCGTGTTGC CATGGAGGGC GATCTTACCA GGCTACAAAT GGCCCTACAA 
GAGAAGGACT GCAGCATTCG TCAAATGGCT GAGCGGCTGG AGAACCAAAA CCGTGCCTTG 
ACCCAATTGG AGGATCGCTG CACCGCCCTT AAGTCCACCG TCGATCAGCT GAAGGAGCGC 
CTCCAGAAGT CCGCTGTGAG TGAAACCCAA CTGCGGGGCG AGATAAAGAC GCTCCAAAAG 
GAGCTTTCCG AGCAGGGTCA CTGCTCCCAG GCCAACGAGG ATAAGCTGAA GTTGGTGCAA 
AAGTCTCTGC AGACCGCTGA GAACGAGAAG CGCATCCTCA CCGAGCGCCT AGATAGCGCT 
CAGACCAATC TCAACGAGCT GCGACGTAGC CAACAGGCCC AGCTGGATGG TAACCAGCGC 
CTGCAGGAAC AGGTGACCGA TCTGGAGGTT CAGCGTTCGG CACTGGAGTC CCAACTTCGG 
ATTGCCAAGT GGAACCAGGA GAGTGGCGGC GACAAGGATC TGACAAACGG CAACGGAGGC 
GGAAATGGCG AGGAGGAGCT CAGCAGGCAG CTGAAGTCCT CGCAGCGGGA AAAGTCGGAG 
CTACGCAGCA AACTGCAAAC CCTACAGGAC AAAGTCAAAC AGTTGGAGTG CGACCGGAAA 
AGCAAGTTTT CGGGTGGAAA CGCCTATGAT CGGGCTGAGA AGTCCAACTC CTTTTACGGT 
GGCGCAGCTG AATCCGGCGA GTTCGACTCC AATCGCTACG ATGTGGGTGG AGGAAATGCT 
GGCGGAGGCT CTTTCAACTG CGGATTGGAT CACAGTGTAA TCGAGCAGGA GACGCGCGAT 
CTGCGACTCA AGGTGCGCCG CCTGGAGACA TTGTTGGCGG AGAAGGAGTC CGAGTTGGCG 
CGCTGCAAGG CGCGAATGAA CGACAGCGCC AAGTGCCATG ATGGCTTAGA TGGAGATCGT 
TATCGCAGTG CTCAGATGCA CGCAGAGAAG CTTCTTGACG CCAGGGAGCA GTCGCATCGG 
CAACAAGTGC TGCGCCTGGA GAACCAGATC TCAATGCTGC GCGAGCAGTT GGCCCAGGAG 
GCCAAACGGC GACAGCAGTA CATTCTACGC AGCTCGAAGG CCAATAGAGA AATGCAGCAT 
CTGAGGAGCA CCCTAGGAGA TTCCCTGCGC AATGTCTCCC AGCACCCGGT GGATCCCCAT 
CTCTTGGAAA GCGAGAGCCG GCGTCTCGAC TCGGCCGTTT CAATGAGCCT GCCACCGTCG 
AGCTGCCGGG ACTATGATCG CGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Root cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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