spas cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spas
Entrez Gene ID	42846
Full Name	spastin
Synonyms	CG5977,D-spastin,Dmel\CG5977,Dspastin,Spas,Spastin
General protein information	Preferred Names spastin Names CG5977 gene product from transcript CG5977-RD CG5977-PA CG5977-PB CG5977-PC CG5977-PD CG5977-PE CG5977-PF D-spastin Dm-Spastin dspastin spas-PA spas-PB spas-PC spas-PD spas-PE spas-PF spasin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95D9-95D9

mRNA and Protein(s)

mRNA	Protein	Name
NM_170115.3	NP_732941.2	spastin, isoform A
NM_001300565.1	NP_001287494.1	spastin, isoform D
NM_001316509.1	NP_001303438.1	spastin, isoform E
NM_001316508.1	NP_001303437.1	spastin, isoform F
NM_142949.4	NP_651206.3	spastin, isoform B
NM_001275967.2	NP_001262896.1	spastin, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPAST	NP_055761.2
Oryza sativa (rice)	Os06g0130000	NP_001056686.1
Xenopus tropicalis (tropical clawed frog)	spast	NP_001016453.1
	SPAST	XP_002799248.1
Canis lupus familiaris (dog)	SPAST	XP_850973.1
Drosophila melanogaster (fruit fly)	spas	NP_651206.3
Pan troglodytes (chimpanzee)	SPAST	XP_001164367.1
Mus musculus (house mouse)	Spast	NP_001156342.1
Rattus norvegicus (Norway rat)	Spast	NP_001102172.2
Danio rerio (zebrafish)	spast	NP_998080.1
Arabidopsis thaliana (thale cress)	AT2G45500	NP_182074.4
Bos taurus (cattle)	SPAST	NP_001075060.1
Gallus gallus (chicken)	SPAST	NP_001026232.1
Anopheles gambiae (African malaria mosquito)	SPAST_ANOGA	XP_312634.5
Caenorhabditis elegans (roundworm)	spas-1	NP_741586.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

targeting sequence directs spastin to the surface of lipid droplets. Depletion of the homologous spastin proteins in both flies and worms affects lipid droplet number and triacylglycerol content.
Title: Spastin binds to lipid droplets and affects lipid metabolism.

The spastin is a key regulator of axon regeneration, and initiation of regeneration is extremely sensitive to the spastin copy number.
Title: Normal spastin gene dosage is specifically required for axon regeneration.

analysis of spastin's microtubule-binding properties and comparison with katanin
Title: Spastin's microtubule-binding properties and comparison to katanin.

Dendritic growth is regulated by Kruppel-like factor dendritic arbor reduction protein 1 (Dar1) suppressing the expression of microtubule-severing protein spastin.
Title: Differential regulation of dendritic and axonal development by the novel Kr?ppel-like factor Dar1.

spastin is essential for apical domain biogenesis during rhabdomere elongation in Drosophila photoreceptor morphogenesis
Title: Role of spastin in apical domain control along the rhabdomere elongation in Drosophila photoreceptor.

The following spas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa41225	NM_001316508.1 Latest version!	Drosophila melanogaster spastin (spas), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OFa05882	NM_170115.3 Latest version!	Drosophila melanogaster spastin (spas), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa05882	NM_142949.4 Latest version!	Drosophila melanogaster spastin (spas), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa08716	NM_001275967.2 Latest version!	Drosophila melanogaster spastin (spas), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa08716	NM_001300565.1 Latest version!	Drosophila melanogaster spastin (spas), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa41226	NM_001316509.1 Latest version!	Drosophila melanogaster spastin (spas), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spas ( NM_001316508.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303437.1 Drosophila melanogaster spastin (spas), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa41225
Clone ID Related Accession (Same CDS sequence)	NM_001316508.1
Accession Version	NM_001316508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spastin, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCCA ATCGTCCGGG AGGAGGGTAT TCACCTGGTC CCGGCGATCC ATTGCTGGCC 
AAGCAGAAGC ATCACCATCG ACGCGCCTTC GAGTACATCT CCAAGGCGCT CAAAATTGAT 
GAGGAGAACG AAGGTCACAA AGAGCTGGCA ATTGAGCTCT ACCGAAAAGG TATCAAGGAG 
CTAGAGGATG GCATAGCTGT CGATTGCTGG AGTGGACGTG GTGATGTGTG GGATCGGGCT 
CAGCGCCTGC ACGACAAGAT GCAGACAAAC CTTTCGATGG CGCGGGATCG TCTCCATTTT 
CTAGCATCTG GCCGCAAACT GACAATTGGA TCCAAACGAC CCGTCAACTT GGCCGTGGCC 
AACAAATCAC AAACGCTGCC CCGCAACCTT GGCTCCAAAA CATCTGTAGG CGCAGTCCAA 
AGGCAACCAG CCAAGACAGC TGCAACGCCT CCCGCCGTTC GGCGACAATT TTCTTCGGGG 
CGTAATACGC CGCCCCAGCG TTCGCGGACT CCCATAAACA ACAACGGACC CAGTGGCAGT 
GGCGCCAGCA CTCCGGTGGT TAGTGTGAAG GGAGTGGAAC AGAAGCTGGT GCAACTTATA 
CTCGACGAGA TCGTCGAGGG TGGGGCCAAA GTGGAGTGGA CTGACATTGC CGGTCAGGAT 
GTGGCCAAGC AGGCTCTACA GGAAATGGTC ATTCTACCCT CCGTCCGACC GGAACTCTTT 
ACGGGGCTTC GTGCACCGGC TAAGGGTTTA TTGCTGTTTG GTCCTCCCGG AAATGGCAAG 
ACACTGCTGG CCCGCGCCGT GGCTACTGAG TGTAGTGCCA CCTTCCTGAA CATTTCGGCT 
GCCTCGCTGA CCAGCAAGTA CGTGGGGGAT GGCGAGAAAC TGGTGAGGGC TCTCTTCGCC 
GTGGCCCGTC ACATGCAGCC CTCCATTATT TTCATCGACG AGGTGGACTC GTTGCTTTCA 
GAGCGAAGCA GCAGCGAGCA TGAAGCATCG CGTCGCCTAA AGACCGAGTT TCTAGTGGAG 
TTCGATGGGC TGCCTGGAAA TCCAGACGGA GACAGGATCG TGGTGCTGGC CGCCACCAAT 
CGACCGCAGG AATTGGACGA GGCAGCCCTG CGTCGGTTCA CAAAGCGCGT TTACGTCTCA 
CTGCCCGACG AGCAAACCCG CGAGCTGCTC CTCAATCGAC TGCTCCAGAA GCAAGGCAGT 
CCGTTGGATA CCGAGGCGTT GCGTCGCCTT GCAAAGATAA CAGACGGATA CTCGGGCTCC 
GACTTAACGG CACTGGCCAA GGACGCCGCA TTGGAGCCCA TTCGAGAGTT GAATGTGGAG 
CAAGTGAAGT GTCTGGACAT CAGTGCAATG CGTGCAATCA CAGAGCAGGA CTTTCACAGT 
TCGCTCAAGC GCATCAGGCG CTCGGTGGCG CCGCAAAGCC TCAACTCATA TGAGAAGTGG 
TCGCAAGATT ATGGCGACAT CACCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303437.1
CDS	916..2385
Translation	MAANRPGGGYSPGPGDPLLAKQKHHHRRAFEYISKALKIDEENEGHKELAIELYRKGIKELEDGIAVDCWSGRGDVWDRAQRLHDKMQTNLSMARDRLHFLASGRKLTIGSKRPVNLAVANKSQTLPRNLGSKTSVGAVQRQPAKTAATPPAVRRQFSSGRNTPPQRSRTPINNNGPSGSGASTPVVSVKGVEQKLVQLILDEIVEGGAKVEWTDIAGQDVAKQALQEMVILPSVRPELFTGLRAPAKGLLLFGPPGNGKTLLARAVATECSATFLNISAASLTSKYVGDGEKLVRALFAVARHMQPSIIFIDEVDSLLSERSSSEHEASRRLKTEFLVEFDGLPGNPDGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPDEQTRELLLNRLLQKQGSPLDTEALRRLAKITDGYSGSDLTALAKDAALEPIRELNVEQVKCLDISAMRAITEQDFHSSLKRIRRSVAPQSLNSYEKWSQDYGDITI

Target ORF information:

RefSeq Version	NM_001316508.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spastin (spas), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316508.1

ORF Insert Sequence:

ATGGCCGCCA ATCGTCCGGG AGGAGGGTAT TCACCTGGTC CCGGCGATCC ATTGCTGGCC 
AAGCAGAAGC ATCACCATCG ACGCGCCTTC GAGTACATCT CCAAGGCGCT CAAAATTGAT 
GAGGAGAACG AAGGTCACAA AGAGCTGGCA ATTGAGCTCT ACCGAAAAGG TATCAAGGAG 
CTAGAGGATG GCATAGCTGT CGATTGCTGG AGTGGACGTG GTGATGTGTG GGATCGGGCT 
CAGCGCCTGC ACGACAAGAT GCAGACAAAC CTTTCGATGG CGCGGGATCG TCTCCATTTT 
CTAGCATCTG GCCGCAAACT GACAATTGGA TCCAAACGAC CCGTCAACTT GGCCGTGGCC 
AACAAATCAC AAACGCTGCC CCGCAACCTT GGCTCCAAAA CATCTGTAGG CGCAGTCCAA 
AGGCAACCAG CCAAGACAGC TGCAACGCCT CCCGCCGTTC GGCGACAATT TTCTTCGGGG 
CGTAATACGC CGCCCCAGCG TTCGCGGACT CCCATAAACA ACAACGGACC CAGTGGCAGT 
GGCGCCAGCA CTCCGGTGGT TAGTGTGAAG GGAGTGGAAC AGAAGCTGGT GCAACTTATA 
CTCGACGAGA TCGTCGAGGG TGGGGCCAAA GTGGAGTGGA CTGACATTGC CGGTCAGGAT 
GTGGCCAAGC AGGCTCTACA GGAAATGGTC ATTCTACCCT CCGTCCGACC GGAACTCTTT 
ACGGGGCTTC GTGCACCGGC TAAGGGTTTA TTGCTGTTTG GTCCTCCCGG AAATGGCAAG 
ACACTGCTGG CCCGCGCCGT GGCTACTGAG TGTAGTGCCA CCTTCCTGAA CATTTCGGCT 
GCCTCGCTGA CCAGCAAGTA CGTGGGGGAT GGCGAGAAAC TGGTGAGGGC TCTCTTCGCC 
GTGGCCCGTC ACATGCAGCC CTCCATTATT TTCATCGACG AGGTGGACTC GTTGCTTTCA 
GAGCGAAGCA GCAGCGAGCA TGAAGCATCG CGTCGCCTAA AGACCGAGTT TCTAGTGGAG 
TTCGATGGGC TGCCTGGAAA TCCAGACGGA GACAGGATCG TGGTGCTGGC CGCCACCAAT 
CGACCGCAGG AATTGGACGA GGCAGCCCTG CGTCGGTTCA CAAAGCGCGT TTACGTCTCA 
CTGCCCGACG AGCAAACCCG CGAGCTGCTC CTCAATCGAC TGCTCCAGAA GCAAGGCAGT 
CCGTTGGATA CCGAGGCGTT GCGTCGCCTT GCAAAGATAA CAGACGGATA CTCGGGCTCC 
GACTTAACGG CACTGGCCAA GGACGCCGCA TTGGAGCCCA TTCGAGAGTT GAATGTGGAG 
CAAGTGAAGT GTCTGGACAT CAGTGCAATG CGTGCAATCA CAGAGCAGGA CTTTCACAGT 
TCGCTCAAGC GCATCAGGCG CTCGGTGGCG CCGCAAAGCC TCAACTCATA TGAGAAGTGG 
TCGCAAGATT ATGGCGACAT CACCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spas ( NM_170115.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732941.2 Drosophila melanogaster spastin (spas), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05882
Clone ID Related Accession (Same CDS sequence)	NM_170115.3 , NM_142949.4
Accession Version	NM_170115.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spastin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170115.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTACGCA CTAAAAACCA GTCGTCCTCC TCCAGCGCCA GCAGCAGCAG CACCAAGTCA 
CCAATAAAGT CCAGCAGCGG GGCAGGATCA TCCGGCGGAG GACTCGGTGG TCGCCAGTCC 
ACTCACCGCT CGTCGAGCGC CTCAAACGTT GCTGCCGTTG TTGCCGGCGG TTCATCTGCC 
GCCGGTGGAG GCTCCTCATC GAATCGCCGT AGTCCGGGCA GCTCACCCGA CGGCGACGAC 
GACACCACCA CCACAGATGA CCTGACCCCC ACAACGTGCT CCCCACGCAG CGGCCACCAC 
CATTCGTACG GCGGCTACTC GTCCTCTGTG CACAAACAGA ACCTCTACGT CGTCTCGTTC 
CCCATAATCT TTCTTTTCAA CGTTCTGCGC TCGCTGATTT ATCAGCTGTT TTGTATTTTT 
CGCTACCTAT ACGGTGCGAG CACAAAGGTG ATATACCGCC CGCACCGACG CGACTGTAAT 
ATTGAAATCG TGGTGCAGAA TTCCTCCAAG GAGCAACAGC AGTCGTTGAA CCATCCTTCG 
GAGCTGAACC GTGAAGGTGA CGGACAGGAA CAGCAGTTAT CCAATCAGCC ACAGCGTTTC 
AGGCCCATTC AACCGTTGGA GATGGCCGCC AATCGTCCGG GAGGAGGGTA TTCACCTGGT 
CCCGGCGATC CATTGCTGGC CAAGCAGAAG CATCACCATC GACGCGCCTT CGAGTACATC 
TCCAAGGCGC TCAAAATTGA TGAGGAGAAC GAAGGTCACA AAGAGCTGGC AATTGAGCTC 
TACCGAAAAG GTATCAAGGA GCTAGAGGAT GGCATAGCTG TCGATTGCTG GAGTGGACGT 
GGTGATGTGT GGGATCGGGC TCAGCGCCTG CACGACAAGA TGCAGACAAA CCTTTCGATG 
GCGCGGGATC GTCTCCATTT TCTAGCTCTG CGTGAGCAGG ATTTGCAAAT GCAGCGCCTC 
TCCTTAAAGG AGAAGCAGAA AGAAGAGGCT CAAAGCAAGC CGCAGAAGAC CAGGGAGCCC 
ATGCTGGCAG GAATGACCAA CGAACCAATG AAACTAAGGG TGCGCAGCAG TGGCTATGGG 
CCCAAGGCCA CCACCAGTGC CCAACCCACT GCATCTGGCC GCAAACTGAC AATTGGATCC 
AAACGACCCG TCAACTTGGC CGTGGCCAAC AAATCACAAA CGCTGCCCCG CAACCTTGGC 
TCCAAAACAT CTGTAGGCGC AGTCCAAAGG CAACCAGCCA AGACAGCTGC AACGCCTCCC 
GCCGTTCGGC GACAATTTTC TTCGGGGCGT AATACGCCGC CCCAGCGTTC GCGGACTCCC 
ATAAACAACA ACGGACCCAG TGGCAGTGGC GCCAGCACTC CGGTGGTTAG TGTGAAGGGA 
GTGGAACAGA AGCTGGTGCA ACTTATACTC GACGAGATCG TCGAGGGTGG GGCCAAAGTG 
GAGTGGACTG ACATTGCCGG TCAGGATGTG GCCAAGCAGG CTCTACAGGA AATGGTCATT 
CTACCCTCCG TCCGACCGGA ACTCTTTACG GGGCTTCGTG CACCGGCTAA GGGTTTATTG 
CTGTTTGGTC CTCCCGGAAA TGGCAAGACA CTGCTGGCCC GCGCCGTGGC TACTGAGTGT 
AGTGCCACCT TCCTGAACAT TTCGGCTGCC TCGCTGACCA GCAAGTACGT GGGGGATGGC 
GAGAAACTGG TGAGGGCTCT CTTCGCCGTG GCCCGTCACA TGCAGCCCTC CATTATTTTC 
ATCGACGAGG TGGACTCGTT GCTTTCAGAG CGAAGCAGCA GCGAGCATGA AGCATCGCGT 
CGCCTAAAGA CCGAGTTTCT AGTGGAGTTC GATGGGCTGC CTGGAAATCC AGACGGAGAC 
AGGATCGTGG TGCTGGCCGC CACCAATCGA CCGCAGGAAT TGGACGAGGC AGCCCTGCGT 
CGGTTCACAA AGCGCGTTTA CGTCTCACTG CCCGACGAGC AAACCCGCGA GCTGCTCCTC 
AATCGACTGC TCCAGAAGCA AGGCAGTCCG TTGGATACCG AGGCGTTGCG TCGCCTTGCA 
AAGATAACAG ACGGATACTC GGGCTCCGAC TTAACGGCAC TGGCCAAGGA CGCCGCATTG 
GAGCCCATTC GAGAGTTGAA TGTGGAGCAA GTGAAGTGTC TGGACATCAG TGCAATGCGT 
GCAATCACAG AGCAGGACTT TCACAGTTCG CTCAAGCGCA TCAGGCGCTC GGTGGCGCCG 
CAAAGCCTCA ACTCATATGA GAAGTGGTCG CAAGATTATG GCGACATCAC CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732941.2
CDS	295..2571
Translation	MVRTKNQSSSSSASSSSTKSPIKSSSGAGSSGGGLGGRQSTHRSSSASNVAAVVAGGSSAAGGGSSSNRRSPGSSPDGDDDTTTTDDLTPTTCSPRSGHHHSYGGYSSSVHKQNLYVVSFPIIFLFNVLRSLIYQLFCIFRYLYGASTKVIYRPHRRDCNIEIVVQNSSKEQQQSLNHPSELNREGDGQEQQLSNQPQRFRPIQPLEMAANRPGGGYSPGPGDPLLAKQKHHHRRAFEYISKALKIDEENEGHKELAIELYRKGIKELEDGIAVDCWSGRGDVWDRAQRLHDKMQTNLSMARDRLHFLALREQDLQMQRLSLKEKQKEEAQSKPQKTREPMLAGMTNEPMKLRVRSSGYGPKATTSAQPTASGRKLTIGSKRPVNLAVANKSQTLPRNLGSKTSVGAVQRQPAKTAATPPAVRRQFSSGRNTPPQRSRTPINNNGPSGSGASTPVVSVKGVEQKLVQLILDEIVEGGAKVEWTDIAGQDVAKQALQEMVILPSVRPELFTGLRAPAKGLLLFGPPGNGKTLLARAVATECSATFLNISAASLTSKYVGDGEKLVRALFAVARHMQPSIIFIDEVDSLLSERSSSEHEASRRLKTEFLVEFDGLPGNPDGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPDEQTRELLLNRLLQKQGSPLDTEALRRLAKITDGYSGSDLTALAKDAALEPIRELNVEQVKCLDISAMRAITEQDFHSSLKRIRRSVAPQSLNSYEKWSQDYGDITI

Target ORF information:

RefSeq Version	NM_170115.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spastin (spas), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170115.3

ORF Insert Sequence:

ATGGTACGCA CTAAAAACCA GTCGTCCTCC TCCAGCGCCA GCAGCAGCAG CACCAAGTCA 
CCAATAAAGT CCAGCAGCGG GGCAGGATCA TCCGGCGGAG GACTCGGTGG TCGCCAGTCC 
ACTCACCGCT CGTCGAGCGC CTCAAACGTT GCTGCCGTTG TTGCCGGCGG TTCATCTGCC 
GCCGGTGGAG GCTCCTCATC GAATCGCCGT AGTCCGGGCA GCTCACCCGA CGGCGACGAC 
GACACCACCA CCACAGATGA CCTGACCCCC ACAACGTGCT CCCCACGCAG CGGCCACCAC 
CATTCGTACG GCGGCTACTC GTCCTCTGTG CACAAACAGA ACCTCTACGT CGTCTCGTTC 
CCCATAATCT TTCTTTTCAA CGTTCTGCGC TCGCTGATTT ATCAGCTGTT TTGTATTTTT 
CGCTACCTAT ACGGTGCGAG CACAAAGGTG ATATACCGCC CGCACCGACG CGACTGTAAT 
ATTGAAATCG TGGTGCAGAA TTCCTCCAAG GAGCAACAGC AGTCGTTGAA CCATCCTTCG 
GAGCTGAACC GTGAAGGTGA CGGACAGGAA CAGCAGTTAT CCAATCAGCC ACAGCGTTTC 
AGGCCCATTC AACCGTTGGA GATGGCCGCC AATCGTCCGG GAGGAGGGTA TTCACCTGGT 
CCCGGCGATC CATTGCTGGC CAAGCAGAAG CATCACCATC GACGCGCCTT CGAGTACATC 
TCCAAGGCGC TCAAAATTGA TGAGGAGAAC GAAGGTCACA AAGAGCTGGC AATTGAGCTC 
TACCGAAAAG GTATCAAGGA GCTAGAGGAT GGCATAGCTG TCGATTGCTG GAGTGGACGT 
GGTGATGTGT GGGATCGGGC TCAGCGCCTG CACGACAAGA TGCAGACAAA CCTTTCGATG 
GCGCGGGATC GTCTCCATTT TCTAGCTCTG CGTGAGCAGG ATTTGCAAAT GCAGCGCCTC 
TCCTTAAAGG AGAAGCAGAA AGAAGAGGCT CAAAGCAAGC CGCAGAAGAC CAGGGAGCCC 
ATGCTGGCAG GAATGACCAA CGAACCAATG AAACTAAGGG TGCGCAGCAG TGGCTATGGG 
CCCAAGGCCA CCACCAGTGC CCAACCCACT GCATCTGGCC GCAAACTGAC AATTGGATCC 
AAACGACCCG TCAACTTGGC CGTGGCCAAC AAATCACAAA CGCTGCCCCG CAACCTTGGC 
TCCAAAACAT CTGTAGGCGC AGTCCAAAGG CAACCAGCCA AGACAGCTGC AACGCCTCCC 
GCCGTTCGGC GACAATTTTC TTCGGGGCGT AATACGCCGC CCCAGCGTTC GCGGACTCCC 
ATAAACAACA ACGGACCCAG TGGCAGTGGC GCCAGCACTC CGGTGGTTAG TGTGAAGGGA 
GTGGAACAGA AGCTGGTGCA ACTTATACTC GACGAGATCG TCGAGGGTGG GGCCAAAGTG 
GAGTGGACTG ACATTGCCGG TCAGGATGTG GCCAAGCAGG CTCTACAGGA AATGGTCATT 
CTACCCTCCG TCCGACCGGA ACTCTTTACG GGGCTTCGTG CACCGGCTAA GGGTTTATTG 
CTGTTTGGTC CTCCCGGAAA TGGCAAGACA CTGCTGGCCC GCGCCGTGGC TACTGAGTGT 
AGTGCCACCT TCCTGAACAT TTCGGCTGCC TCGCTGACCA GCAAGTACGT GGGGGATGGC 
GAGAAACTGG TGAGGGCTCT CTTCGCCGTG GCCCGTCACA TGCAGCCCTC CATTATTTTC 
ATCGACGAGG TGGACTCGTT GCTTTCAGAG CGAAGCAGCA GCGAGCATGA AGCATCGCGT 
CGCCTAAAGA CCGAGTTTCT AGTGGAGTTC GATGGGCTGC CTGGAAATCC AGACGGAGAC 
AGGATCGTGG TGCTGGCCGC CACCAATCGA CCGCAGGAAT TGGACGAGGC AGCCCTGCGT 
CGGTTCACAA AGCGCGTTTA CGTCTCACTG CCCGACGAGC AAACCCGCGA GCTGCTCCTC 
AATCGACTGC TCCAGAAGCA AGGCAGTCCG TTGGATACCG AGGCGTTGCG TCGCCTTGCA 
AAGATAACAG ACGGATACTC GGGCTCCGAC TTAACGGCAC TGGCCAAGGA CGCCGCATTG 
GAGCCCATTC GAGAGTTGAA TGTGGAGCAA GTGAAGTGTC TGGACATCAG TGCAATGCGT 
GCAATCACAG AGCAGGACTT TCACAGTTCG CTCAAGCGCA TCAGGCGCTC GGTGGCGCCG 
CAAAGCCTCA ACTCATATGA GAAGTGGTCG CAAGATTATG GCGACATCAC CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spas ( NM_142949.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651206.3 Drosophila melanogaster spastin (spas), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05882
Clone ID Related Accession (Same CDS sequence)	NM_170115.3 , NM_142949.4
Accession Version	NM_142949.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spastin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142949.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTACGCA CTAAAAACCA GTCGTCCTCC TCCAGCGCCA GCAGCAGCAG CACCAAGTCA 
CCAATAAAGT CCAGCAGCGG GGCAGGATCA TCCGGCGGAG GACTCGGTGG TCGCCAGTCC 
ACTCACCGCT CGTCGAGCGC CTCAAACGTT GCTGCCGTTG TTGCCGGCGG TTCATCTGCC 
GCCGGTGGAG GCTCCTCATC GAATCGCCGT AGTCCGGGCA GCTCACCCGA CGGCGACGAC 
GACACCACCA CCACAGATGA CCTGACCCCC ACAACGTGCT CCCCACGCAG CGGCCACCAC 
CATTCGTACG GCGGCTACTC GTCCTCTGTG CACAAACAGA ACCTCTACGT CGTCTCGTTC 
CCCATAATCT TTCTTTTCAA CGTTCTGCGC TCGCTGATTT ATCAGCTGTT TTGTATTTTT 
CGCTACCTAT ACGGTGCGAG CACAAAGGTG ATATACCGCC CGCACCGACG CGACTGTAAT 
ATTGAAATCG TGGTGCAGAA TTCCTCCAAG GAGCAACAGC AGTCGTTGAA CCATCCTTCG 
GAGCTGAACC GTGAAGGTGA CGGACAGGAA CAGCAGTTAT CCAATCAGCC ACAGCGTTTC 
AGGCCCATTC AACCGTTGGA GATGGCCGCC AATCGTCCGG GAGGAGGGTA TTCACCTGGT 
CCCGGCGATC CATTGCTGGC CAAGCAGAAG CATCACCATC GACGCGCCTT CGAGTACATC 
TCCAAGGCGC TCAAAATTGA TGAGGAGAAC GAAGGTCACA AAGAGCTGGC AATTGAGCTC 
TACCGAAAAG GTATCAAGGA GCTAGAGGAT GGCATAGCTG TCGATTGCTG GAGTGGACGT 
GGTGATGTGT GGGATCGGGC TCAGCGCCTG CACGACAAGA TGCAGACAAA CCTTTCGATG 
GCGCGGGATC GTCTCCATTT TCTAGCTCTG CGTGAGCAGG ATTTGCAAAT GCAGCGCCTC 
TCCTTAAAGG AGAAGCAGAA AGAAGAGGCT CAAAGCAAGC CGCAGAAGAC CAGGGAGCCC 
ATGCTGGCAG GAATGACCAA CGAACCAATG AAACTAAGGG TGCGCAGCAG TGGCTATGGG 
CCCAAGGCCA CCACCAGTGC CCAACCCACT GCATCTGGCC GCAAACTGAC AATTGGATCC 
AAACGACCCG TCAACTTGGC CGTGGCCAAC AAATCACAAA CGCTGCCCCG CAACCTTGGC 
TCCAAAACAT CTGTAGGCGC AGTCCAAAGG CAACCAGCCA AGACAGCTGC AACGCCTCCC 
GCCGTTCGGC GACAATTTTC TTCGGGGCGT AATACGCCGC CCCAGCGTTC GCGGACTCCC 
ATAAACAACA ACGGACCCAG TGGCAGTGGC GCCAGCACTC CGGTGGTTAG TGTGAAGGGA 
GTGGAACAGA AGCTGGTGCA ACTTATACTC GACGAGATCG TCGAGGGTGG GGCCAAAGTG 
GAGTGGACTG ACATTGCCGG TCAGGATGTG GCCAAGCAGG CTCTACAGGA AATGGTCATT 
CTACCCTCCG TCCGACCGGA ACTCTTTACG GGGCTTCGTG CACCGGCTAA GGGTTTATTG 
CTGTTTGGTC CTCCCGGAAA TGGCAAGACA CTGCTGGCCC GCGCCGTGGC TACTGAGTGT 
AGTGCCACCT TCCTGAACAT TTCGGCTGCC TCGCTGACCA GCAAGTACGT GGGGGATGGC 
GAGAAACTGG TGAGGGCTCT CTTCGCCGTG GCCCGTCACA TGCAGCCCTC CATTATTTTC 
ATCGACGAGG TGGACTCGTT GCTTTCAGAG CGAAGCAGCA GCGAGCATGA AGCATCGCGT 
CGCCTAAAGA CCGAGTTTCT AGTGGAGTTC GATGGGCTGC CTGGAAATCC AGACGGAGAC 
AGGATCGTGG TGCTGGCCGC CACCAATCGA CCGCAGGAAT TGGACGAGGC AGCCCTGCGT 
CGGTTCACAA AGCGCGTTTA CGTCTCACTG CCCGACGAGC AAACCCGCGA GCTGCTCCTC 
AATCGACTGC TCCAGAAGCA AGGCAGTCCG TTGGATACCG AGGCGTTGCG TCGCCTTGCA 
AAGATAACAG ACGGATACTC GGGCTCCGAC TTAACGGCAC TGGCCAAGGA CGCCGCATTG 
GAGCCCATTC GAGAGTTGAA TGTGGAGCAA GTGAAGTGTC TGGACATCAG TGCAATGCGT 
GCAATCACAG AGCAGGACTT TCACAGTTCG CTCAAGCGCA TCAGGCGCTC GGTGGCGCCG 
CAAAGCCTCA ACTCATATGA GAAGTGGTCG CAAGATTATG GCGACATCAC CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651206.3
CDS	295..2571
Translation	MVRTKNQSSSSSASSSSTKSPIKSSSGAGSSGGGLGGRQSTHRSSSASNVAAVVAGGSSAAGGGSSSNRRSPGSSPDGDDDTTTTDDLTPTTCSPRSGHHHSYGGYSSSVHKQNLYVVSFPIIFLFNVLRSLIYQLFCIFRYLYGASTKVIYRPHRRDCNIEIVVQNSSKEQQQSLNHPSELNREGDGQEQQLSNQPQRFRPIQPLEMAANRPGGGYSPGPGDPLLAKQKHHHRRAFEYISKALKIDEENEGHKELAIELYRKGIKELEDGIAVDCWSGRGDVWDRAQRLHDKMQTNLSMARDRLHFLALREQDLQMQRLSLKEKQKEEAQSKPQKTREPMLAGMTNEPMKLRVRSSGYGPKATTSAQPTASGRKLTIGSKRPVNLAVANKSQTLPRNLGSKTSVGAVQRQPAKTAATPPAVRRQFSSGRNTPPQRSRTPINNNGPSGSGASTPVVSVKGVEQKLVQLILDEIVEGGAKVEWTDIAGQDVAKQALQEMVILPSVRPELFTGLRAPAKGLLLFGPPGNGKTLLARAVATECSATFLNISAASLTSKYVGDGEKLVRALFAVARHMQPSIIFIDEVDSLLSERSSSEHEASRRLKTEFLVEFDGLPGNPDGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPDEQTRELLLNRLLQKQGSPLDTEALRRLAKITDGYSGSDLTALAKDAALEPIRELNVEQVKCLDISAMRAITEQDFHSSLKRIRRSVAPQSLNSYEKWSQDYGDITI

Target ORF information:

RefSeq Version	NM_142949.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spastin (spas), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142949.4

ORF Insert Sequence:

ATGGTACGCA CTAAAAACCA GTCGTCCTCC TCCAGCGCCA GCAGCAGCAG CACCAAGTCA 
CCAATAAAGT CCAGCAGCGG GGCAGGATCA TCCGGCGGAG GACTCGGTGG TCGCCAGTCC 
ACTCACCGCT CGTCGAGCGC CTCAAACGTT GCTGCCGTTG TTGCCGGCGG TTCATCTGCC 
GCCGGTGGAG GCTCCTCATC GAATCGCCGT AGTCCGGGCA GCTCACCCGA CGGCGACGAC 
GACACCACCA CCACAGATGA CCTGACCCCC ACAACGTGCT CCCCACGCAG CGGCCACCAC 
CATTCGTACG GCGGCTACTC GTCCTCTGTG CACAAACAGA ACCTCTACGT CGTCTCGTTC 
CCCATAATCT TTCTTTTCAA CGTTCTGCGC TCGCTGATTT ATCAGCTGTT TTGTATTTTT 
CGCTACCTAT ACGGTGCGAG CACAAAGGTG ATATACCGCC CGCACCGACG CGACTGTAAT 
ATTGAAATCG TGGTGCAGAA TTCCTCCAAG GAGCAACAGC AGTCGTTGAA CCATCCTTCG 
GAGCTGAACC GTGAAGGTGA CGGACAGGAA CAGCAGTTAT CCAATCAGCC ACAGCGTTTC 
AGGCCCATTC AACCGTTGGA GATGGCCGCC AATCGTCCGG GAGGAGGGTA TTCACCTGGT 
CCCGGCGATC CATTGCTGGC CAAGCAGAAG CATCACCATC GACGCGCCTT CGAGTACATC 
TCCAAGGCGC TCAAAATTGA TGAGGAGAAC GAAGGTCACA AAGAGCTGGC AATTGAGCTC 
TACCGAAAAG GTATCAAGGA GCTAGAGGAT GGCATAGCTG TCGATTGCTG GAGTGGACGT 
GGTGATGTGT GGGATCGGGC TCAGCGCCTG CACGACAAGA TGCAGACAAA CCTTTCGATG 
GCGCGGGATC GTCTCCATTT TCTAGCTCTG CGTGAGCAGG ATTTGCAAAT GCAGCGCCTC 
TCCTTAAAGG AGAAGCAGAA AGAAGAGGCT CAAAGCAAGC CGCAGAAGAC CAGGGAGCCC 
ATGCTGGCAG GAATGACCAA CGAACCAATG AAACTAAGGG TGCGCAGCAG TGGCTATGGG 
CCCAAGGCCA CCACCAGTGC CCAACCCACT GCATCTGGCC GCAAACTGAC AATTGGATCC 
AAACGACCCG TCAACTTGGC CGTGGCCAAC AAATCACAAA CGCTGCCCCG CAACCTTGGC 
TCCAAAACAT CTGTAGGCGC AGTCCAAAGG CAACCAGCCA AGACAGCTGC AACGCCTCCC 
GCCGTTCGGC GACAATTTTC TTCGGGGCGT AATACGCCGC CCCAGCGTTC GCGGACTCCC 
ATAAACAACA ACGGACCCAG TGGCAGTGGC GCCAGCACTC CGGTGGTTAG TGTGAAGGGA 
GTGGAACAGA AGCTGGTGCA ACTTATACTC GACGAGATCG TCGAGGGTGG GGCCAAAGTG 
GAGTGGACTG ACATTGCCGG TCAGGATGTG GCCAAGCAGG CTCTACAGGA AATGGTCATT 
CTACCCTCCG TCCGACCGGA ACTCTTTACG GGGCTTCGTG CACCGGCTAA GGGTTTATTG 
CTGTTTGGTC CTCCCGGAAA TGGCAAGACA CTGCTGGCCC GCGCCGTGGC TACTGAGTGT 
AGTGCCACCT TCCTGAACAT TTCGGCTGCC TCGCTGACCA GCAAGTACGT GGGGGATGGC 
GAGAAACTGG TGAGGGCTCT CTTCGCCGTG GCCCGTCACA TGCAGCCCTC CATTATTTTC 
ATCGACGAGG TGGACTCGTT GCTTTCAGAG CGAAGCAGCA GCGAGCATGA AGCATCGCGT 
CGCCTAAAGA CCGAGTTTCT AGTGGAGTTC GATGGGCTGC CTGGAAATCC AGACGGAGAC 
AGGATCGTGG TGCTGGCCGC CACCAATCGA CCGCAGGAAT TGGACGAGGC AGCCCTGCGT 
CGGTTCACAA AGCGCGTTTA CGTCTCACTG CCCGACGAGC AAACCCGCGA GCTGCTCCTC 
AATCGACTGC TCCAGAAGCA AGGCAGTCCG TTGGATACCG AGGCGTTGCG TCGCCTTGCA 
AAGATAACAG ACGGATACTC GGGCTCCGAC TTAACGGCAC TGGCCAAGGA CGCCGCATTG 
GAGCCCATTC GAGAGTTGAA TGTGGAGCAA GTGAAGTGTC TGGACATCAG TGCAATGCGT 
GCAATCACAG AGCAGGACTT TCACAGTTCG CTCAAGCGCA TCAGGCGCTC GGTGGCGCCG 
CAAAGCCTCA ACTCATATGA GAAGTGGTCG CAAGATTATG GCGACATCAC CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spas ( NM_001300565.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287494.1 Drosophila melanogaster spastin (spas), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa08716
Clone ID Related Accession (Same CDS sequence)	NM_001300565.1 , NM_001275967.2
Accession Version	NM_001300565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spastin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTACGCA CTAAAAACCA GTCGTCCTCC TCCAGCGCCA GCAGCAGCAG CACCAAGTCA 
CCAATAAAGT CCAGCAGCGG GGCAGGATCA TCCGGCGGAG GACTCGGTGG TCGCCAGTCC 
ACTCACCGCT CGTCGAGCGC CTCAAACGTT GCTGCCGTTG TTGCCGGCGG TTCATCTGCC 
GCCGGTGGAG GCTCCTCATC GAATCGCCGT AGTCCGGGCA GCTCACCCGA CGGCGACGAC 
GACACCACCA CCACAGATGA CCTGACCCCC ACAACGTGCT CCCCACGCAG CGGCCACCAC 
CATTCGTACG GCGGCTACTC GTCCTCTGTG CACAAACAGA ACCTCTACGT CGTCTCGTTC 
CCCATAATCT TTCTTTTCAA CGTTCTGCGC TCGCTGATTT ATCAGCTGTT TTGTATTTTT 
CGCTACCTAT ACGGTGCGAG CACAAAGGTG ATATACCGCC CGCACCGACG CGACTGTAAT 
ATTGAAATCG TGGTGCAGAA TTCCTCCAAG GAGCAACAGC AGTCGTTGAA CCATCCTTCG 
GAGCTGAACC GTGAAGGTGA CGGACAGGAA CAGCAGTTAT CCAATCAGCC ACAGCGTTTC 
AGGCCCATTC AACCGTTGGA GATGGCCGCC AATCGTCCGG GAGGAGGGTA TTCACCTGGT 
CCCGGCGATC CATTGCTGGC CAAGCAGAAG CATCACCATC GACGCGCCTT CGAGTACATC 
TCCAAGGCGC TCAAAATTGA TGAGGAGAAC GAAGGTCACA AAGAGCTGGC AATTGAGCTC 
TACCGAAAAG GTATCAAGGA GCTAGAGGAT GGCATAGCTG TCGATTGCTG GAGTGGACGT 
GGTGATGTGT GGGATCGGGC TCAGCGCCTG CACGACAAGA TGCAGACAAA CCTTTCGATG 
GCGCGGGATC GTCTCCATTT TCTAGCATCT GGCCGCAAAC TGACAATTGG ATCCAAACGA 
CCCGTCAACT TGGCCGTGGC CAACAAATCA CAAACGCTGC CCCGCAACCT TGGCTCCAAA 
ACATCTGTAG GCGCAGTCCA AAGGCAACCA GCCAAGACAG CTGCAACGCC TCCCGCCGTT 
CGGCGACAAT TTTCTTCGGG GCGTAATACG CCGCCCCAGC GTTCGCGGAC TCCCATAAAC 
AACAACGGAC CCAGTGGCAG TGGCGCCAGC ACTCCGGTGG TTAGTGTGAA GGGAGTGGAA 
CAGAAGCTGG TGCAACTTAT ACTCGACGAG ATCGTCGAGG GTGGGGCCAA AGTGGAGTGG 
ACTGACATTG CCGGTCAGGA TGTGGCCAAG CAGGCTCTAC AGGAAATGGT CATTCTACCC 
TCCGTCCGAC CGGAACTCTT TACGGGGCTT CGTGCACCGG CTAAGGGTTT ATTGCTGTTT 
GGTCCTCCCG GAAATGGCAA GACACTGCTG GCCCGCGCCG TGGCTACTGA GTGTAGTGCC 
ACCTTCCTGA ACATTTCGGC TGCCTCGCTG ACCAGCAAGT ACGTGGGGGA TGGCGAGAAA 
CTGGTGAGGG CTCTCTTCGC CGTGGCCCGT CACATGCAGC CCTCCATTAT TTTCATCGAC 
GAGGTGGACT CGTTGCTTTC AGAGCGAAGC AGCAGCGAGC ATGAAGCATC GCGTCGCCTA 
AAGACCGAGT TTCTAGTGGA GTTCGATGGG CTGCCTGGAA ATCCAGACGG AGACAGGATC 
GTGGTGCTGG CCGCCACCAA TCGACCGCAG GAATTGGACG AGGCAGCCCT GCGTCGGTTC 
ACAAAGCGCG TTTACGTCTC ACTGCCCGAC GAGCAAACCC GCGAGCTGCT CCTCAATCGA 
CTGCTCCAGA AGCAAGGCAG TCCGTTGGAT ACCGAGGCGT TGCGTCGCCT TGCAAAGATA 
ACAGACGGAT ACTCGGGCTC CGACTTAACG GCACTGGCCA AGGACGCCGC ATTGGAGCCC 
ATTCGAGAGT TGAATGTGGA GCAAGTGAAG TGTCTGGACA TCAGTGCAAT GCGTGCAATC 
ACAGAGCAGG ACTTTCACAG TTCGCTCAAG CGCATCAGGC GCTCGGTGGC GCCGCAAAGC 
CTCAACTCAT ATGAGAAGTG GTCGCAAGAT TATGGCGACA TCACCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287494.1
CDS	295..2385
Translation	MVRTKNQSSSSSASSSSTKSPIKSSSGAGSSGGGLGGRQSTHRSSSASNVAAVVAGGSSAAGGGSSSNRRSPGSSPDGDDDTTTTDDLTPTTCSPRSGHHHSYGGYSSSVHKQNLYVVSFPIIFLFNVLRSLIYQLFCIFRYLYGASTKVIYRPHRRDCNIEIVVQNSSKEQQQSLNHPSELNREGDGQEQQLSNQPQRFRPIQPLEMAANRPGGGYSPGPGDPLLAKQKHHHRRAFEYISKALKIDEENEGHKELAIELYRKGIKELEDGIAVDCWSGRGDVWDRAQRLHDKMQTNLSMARDRLHFLASGRKLTIGSKRPVNLAVANKSQTLPRNLGSKTSVGAVQRQPAKTAATPPAVRRQFSSGRNTPPQRSRTPINNNGPSGSGASTPVVSVKGVEQKLVQLILDEIVEGGAKVEWTDIAGQDVAKQALQEMVILPSVRPELFTGLRAPAKGLLLFGPPGNGKTLLARAVATECSATFLNISAASLTSKYVGDGEKLVRALFAVARHMQPSIIFIDEVDSLLSERSSSEHEASRRLKTEFLVEFDGLPGNPDGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPDEQTRELLLNRLLQKQGSPLDTEALRRLAKITDGYSGSDLTALAKDAALEPIRELNVEQVKCLDISAMRAITEQDFHSSLKRIRRSVAPQSLNSYEKWSQDYGDITI

Target ORF information:

RefSeq Version	NM_001300565.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spastin (spas), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300565.1

ORF Insert Sequence:

ATGGTACGCA CTAAAAACCA GTCGTCCTCC TCCAGCGCCA GCAGCAGCAG CACCAAGTCA 
CCAATAAAGT CCAGCAGCGG GGCAGGATCA TCCGGCGGAG GACTCGGTGG TCGCCAGTCC 
ACTCACCGCT CGTCGAGCGC CTCAAACGTT GCTGCCGTTG TTGCCGGCGG TTCATCTGCC 
GCCGGTGGAG GCTCCTCATC GAATCGCCGT AGTCCGGGCA GCTCACCCGA CGGCGACGAC 
GACACCACCA CCACAGATGA CCTGACCCCC ACAACGTGCT CCCCACGCAG CGGCCACCAC 
CATTCGTACG GCGGCTACTC GTCCTCTGTG CACAAACAGA ACCTCTACGT CGTCTCGTTC 
CCCATAATCT TTCTTTTCAA CGTTCTGCGC TCGCTGATTT ATCAGCTGTT TTGTATTTTT 
CGCTACCTAT ACGGTGCGAG CACAAAGGTG ATATACCGCC CGCACCGACG CGACTGTAAT 
ATTGAAATCG TGGTGCAGAA TTCCTCCAAG GAGCAACAGC AGTCGTTGAA CCATCCTTCG 
GAGCTGAACC GTGAAGGTGA CGGACAGGAA CAGCAGTTAT CCAATCAGCC ACAGCGTTTC 
AGGCCCATTC AACCGTTGGA GATGGCCGCC AATCGTCCGG GAGGAGGGTA TTCACCTGGT 
CCCGGCGATC CATTGCTGGC CAAGCAGAAG CATCACCATC GACGCGCCTT CGAGTACATC 
TCCAAGGCGC TCAAAATTGA TGAGGAGAAC GAAGGTCACA AAGAGCTGGC AATTGAGCTC 
TACCGAAAAG GTATCAAGGA GCTAGAGGAT GGCATAGCTG TCGATTGCTG GAGTGGACGT 
GGTGATGTGT GGGATCGGGC TCAGCGCCTG CACGACAAGA TGCAGACAAA CCTTTCGATG 
GCGCGGGATC GTCTCCATTT TCTAGCATCT GGCCGCAAAC TGACAATTGG ATCCAAACGA 
CCCGTCAACT TGGCCGTGGC CAACAAATCA CAAACGCTGC CCCGCAACCT TGGCTCCAAA 
ACATCTGTAG GCGCAGTCCA AAGGCAACCA GCCAAGACAG CTGCAACGCC TCCCGCCGTT 
CGGCGACAAT TTTCTTCGGG GCGTAATACG CCGCCCCAGC GTTCGCGGAC TCCCATAAAC 
AACAACGGAC CCAGTGGCAG TGGCGCCAGC ACTCCGGTGG TTAGTGTGAA GGGAGTGGAA 
CAGAAGCTGG TGCAACTTAT ACTCGACGAG ATCGTCGAGG GTGGGGCCAA AGTGGAGTGG 
ACTGACATTG CCGGTCAGGA TGTGGCCAAG CAGGCTCTAC AGGAAATGGT CATTCTACCC 
TCCGTCCGAC CGGAACTCTT TACGGGGCTT CGTGCACCGG CTAAGGGTTT ATTGCTGTTT 
GGTCCTCCCG GAAATGGCAA GACACTGCTG GCCCGCGCCG TGGCTACTGA GTGTAGTGCC 
ACCTTCCTGA ACATTTCGGC TGCCTCGCTG ACCAGCAAGT ACGTGGGGGA TGGCGAGAAA 
CTGGTGAGGG CTCTCTTCGC CGTGGCCCGT CACATGCAGC CCTCCATTAT TTTCATCGAC 
GAGGTGGACT CGTTGCTTTC AGAGCGAAGC AGCAGCGAGC ATGAAGCATC GCGTCGCCTA 
AAGACCGAGT TTCTAGTGGA GTTCGATGGG CTGCCTGGAA ATCCAGACGG AGACAGGATC 
GTGGTGCTGG CCGCCACCAA TCGACCGCAG GAATTGGACG AGGCAGCCCT GCGTCGGTTC 
ACAAAGCGCG TTTACGTCTC ACTGCCCGAC GAGCAAACCC GCGAGCTGCT CCTCAATCGA 
CTGCTCCAGA AGCAAGGCAG TCCGTTGGAT ACCGAGGCGT TGCGTCGCCT TGCAAAGATA 
ACAGACGGAT ACTCGGGCTC CGACTTAACG GCACTGGCCA AGGACGCCGC ATTGGAGCCC 
ATTCGAGAGT TGAATGTGGA GCAAGTGAAG TGTCTGGACA TCAGTGCAAT GCGTGCAATC 
ACAGAGCAGG ACTTTCACAG TTCGCTCAAG CGCATCAGGC GCTCGGTGGC GCCGCAAAGC 
CTCAACTCAT ATGAGAAGTG GTCGCAAGAT TATGGCGACA TCACCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spas ( NM_001275967.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262896.1 Drosophila melanogaster spastin (spas), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa08716
Clone ID Related Accession (Same CDS sequence)	NM_001300565.1 , NM_001275967.2
Accession Version	NM_001275967.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spastin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275967.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTACGCA CTAAAAACCA GTCGTCCTCC TCCAGCGCCA GCAGCAGCAG CACCAAGTCA 
CCAATAAAGT CCAGCAGCGG GGCAGGATCA TCCGGCGGAG GACTCGGTGG TCGCCAGTCC 
ACTCACCGCT CGTCGAGCGC CTCAAACGTT GCTGCCGTTG TTGCCGGCGG TTCATCTGCC 
GCCGGTGGAG GCTCCTCATC GAATCGCCGT AGTCCGGGCA GCTCACCCGA CGGCGACGAC 
GACACCACCA CCACAGATGA CCTGACCCCC ACAACGTGCT CCCCACGCAG CGGCCACCAC 
CATTCGTACG GCGGCTACTC GTCCTCTGTG CACAAACAGA ACCTCTACGT CGTCTCGTTC 
CCCATAATCT TTCTTTTCAA CGTTCTGCGC TCGCTGATTT ATCAGCTGTT TTGTATTTTT 
CGCTACCTAT ACGGTGCGAG CACAAAGGTG ATATACCGCC CGCACCGACG CGACTGTAAT 
ATTGAAATCG TGGTGCAGAA TTCCTCCAAG GAGCAACAGC AGTCGTTGAA CCATCCTTCG 
GAGCTGAACC GTGAAGGTGA CGGACAGGAA CAGCAGTTAT CCAATCAGCC ACAGCGTTTC 
AGGCCCATTC AACCGTTGGA GATGGCCGCC AATCGTCCGG GAGGAGGGTA TTCACCTGGT 
CCCGGCGATC CATTGCTGGC CAAGCAGAAG CATCACCATC GACGCGCCTT CGAGTACATC 
TCCAAGGCGC TCAAAATTGA TGAGGAGAAC GAAGGTCACA AAGAGCTGGC AATTGAGCTC 
TACCGAAAAG GTATCAAGGA GCTAGAGGAT GGCATAGCTG TCGATTGCTG GAGTGGACGT 
GGTGATGTGT GGGATCGGGC TCAGCGCCTG CACGACAAGA TGCAGACAAA CCTTTCGATG 
GCGCGGGATC GTCTCCATTT TCTAGCATCT GGCCGCAAAC TGACAATTGG ATCCAAACGA 
CCCGTCAACT TGGCCGTGGC CAACAAATCA CAAACGCTGC CCCGCAACCT TGGCTCCAAA 
ACATCTGTAG GCGCAGTCCA AAGGCAACCA GCCAAGACAG CTGCAACGCC TCCCGCCGTT 
CGGCGACAAT TTTCTTCGGG GCGTAATACG CCGCCCCAGC GTTCGCGGAC TCCCATAAAC 
AACAACGGAC CCAGTGGCAG TGGCGCCAGC ACTCCGGTGG TTAGTGTGAA GGGAGTGGAA 
CAGAAGCTGG TGCAACTTAT ACTCGACGAG ATCGTCGAGG GTGGGGCCAA AGTGGAGTGG 
ACTGACATTG CCGGTCAGGA TGTGGCCAAG CAGGCTCTAC AGGAAATGGT CATTCTACCC 
TCCGTCCGAC CGGAACTCTT TACGGGGCTT CGTGCACCGG CTAAGGGTTT ATTGCTGTTT 
GGTCCTCCCG GAAATGGCAA GACACTGCTG GCCCGCGCCG TGGCTACTGA GTGTAGTGCC 
ACCTTCCTGA ACATTTCGGC TGCCTCGCTG ACCAGCAAGT ACGTGGGGGA TGGCGAGAAA 
CTGGTGAGGG CTCTCTTCGC CGTGGCCCGT CACATGCAGC CCTCCATTAT TTTCATCGAC 
GAGGTGGACT CGTTGCTTTC AGAGCGAAGC AGCAGCGAGC ATGAAGCATC GCGTCGCCTA 
AAGACCGAGT TTCTAGTGGA GTTCGATGGG CTGCCTGGAA ATCCAGACGG AGACAGGATC 
GTGGTGCTGG CCGCCACCAA TCGACCGCAG GAATTGGACG AGGCAGCCCT GCGTCGGTTC 
ACAAAGCGCG TTTACGTCTC ACTGCCCGAC GAGCAAACCC GCGAGCTGCT CCTCAATCGA 
CTGCTCCAGA AGCAAGGCAG TCCGTTGGAT ACCGAGGCGT TGCGTCGCCT TGCAAAGATA 
ACAGACGGAT ACTCGGGCTC CGACTTAACG GCACTGGCCA AGGACGCCGC ATTGGAGCCC 
ATTCGAGAGT TGAATGTGGA GCAAGTGAAG TGTCTGGACA TCAGTGCAAT GCGTGCAATC 
ACAGAGCAGG ACTTTCACAG TTCGCTCAAG CGCATCAGGC GCTCGGTGGC GCCGCAAAGC 
CTCAACTCAT ATGAGAAGTG GTCGCAAGAT TATGGCGACA TCACCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262896.1
CDS	295..2385
Translation	MVRTKNQSSSSSASSSSTKSPIKSSSGAGSSGGGLGGRQSTHRSSSASNVAAVVAGGSSAAGGGSSSNRRSPGSSPDGDDDTTTTDDLTPTTCSPRSGHHHSYGGYSSSVHKQNLYVVSFPIIFLFNVLRSLIYQLFCIFRYLYGASTKVIYRPHRRDCNIEIVVQNSSKEQQQSLNHPSELNREGDGQEQQLSNQPQRFRPIQPLEMAANRPGGGYSPGPGDPLLAKQKHHHRRAFEYISKALKIDEENEGHKELAIELYRKGIKELEDGIAVDCWSGRGDVWDRAQRLHDKMQTNLSMARDRLHFLASGRKLTIGSKRPVNLAVANKSQTLPRNLGSKTSVGAVQRQPAKTAATPPAVRRQFSSGRNTPPQRSRTPINNNGPSGSGASTPVVSVKGVEQKLVQLILDEIVEGGAKVEWTDIAGQDVAKQALQEMVILPSVRPELFTGLRAPAKGLLLFGPPGNGKTLLARAVATECSATFLNISAASLTSKYVGDGEKLVRALFAVARHMQPSIIFIDEVDSLLSERSSSEHEASRRLKTEFLVEFDGLPGNPDGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPDEQTRELLLNRLLQKQGSPLDTEALRRLAKITDGYSGSDLTALAKDAALEPIRELNVEQVKCLDISAMRAITEQDFHSSLKRIRRSVAPQSLNSYEKWSQDYGDITI

Target ORF information:

RefSeq Version	NM_001275967.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spastin (spas), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275967.2

ORF Insert Sequence:

ATGGTACGCA CTAAAAACCA GTCGTCCTCC TCCAGCGCCA GCAGCAGCAG CACCAAGTCA 
CCAATAAAGT CCAGCAGCGG GGCAGGATCA TCCGGCGGAG GACTCGGTGG TCGCCAGTCC 
ACTCACCGCT CGTCGAGCGC CTCAAACGTT GCTGCCGTTG TTGCCGGCGG TTCATCTGCC 
GCCGGTGGAG GCTCCTCATC GAATCGCCGT AGTCCGGGCA GCTCACCCGA CGGCGACGAC 
GACACCACCA CCACAGATGA CCTGACCCCC ACAACGTGCT CCCCACGCAG CGGCCACCAC 
CATTCGTACG GCGGCTACTC GTCCTCTGTG CACAAACAGA ACCTCTACGT CGTCTCGTTC 
CCCATAATCT TTCTTTTCAA CGTTCTGCGC TCGCTGATTT ATCAGCTGTT TTGTATTTTT 
CGCTACCTAT ACGGTGCGAG CACAAAGGTG ATATACCGCC CGCACCGACG CGACTGTAAT 
ATTGAAATCG TGGTGCAGAA TTCCTCCAAG GAGCAACAGC AGTCGTTGAA CCATCCTTCG 
GAGCTGAACC GTGAAGGTGA CGGACAGGAA CAGCAGTTAT CCAATCAGCC ACAGCGTTTC 
AGGCCCATTC AACCGTTGGA GATGGCCGCC AATCGTCCGG GAGGAGGGTA TTCACCTGGT 
CCCGGCGATC CATTGCTGGC CAAGCAGAAG CATCACCATC GACGCGCCTT CGAGTACATC 
TCCAAGGCGC TCAAAATTGA TGAGGAGAAC GAAGGTCACA AAGAGCTGGC AATTGAGCTC 
TACCGAAAAG GTATCAAGGA GCTAGAGGAT GGCATAGCTG TCGATTGCTG GAGTGGACGT 
GGTGATGTGT GGGATCGGGC TCAGCGCCTG CACGACAAGA TGCAGACAAA CCTTTCGATG 
GCGCGGGATC GTCTCCATTT TCTAGCATCT GGCCGCAAAC TGACAATTGG ATCCAAACGA 
CCCGTCAACT TGGCCGTGGC CAACAAATCA CAAACGCTGC CCCGCAACCT TGGCTCCAAA 
ACATCTGTAG GCGCAGTCCA AAGGCAACCA GCCAAGACAG CTGCAACGCC TCCCGCCGTT 
CGGCGACAAT TTTCTTCGGG GCGTAATACG CCGCCCCAGC GTTCGCGGAC TCCCATAAAC 
AACAACGGAC CCAGTGGCAG TGGCGCCAGC ACTCCGGTGG TTAGTGTGAA GGGAGTGGAA 
CAGAAGCTGG TGCAACTTAT ACTCGACGAG ATCGTCGAGG GTGGGGCCAA AGTGGAGTGG 
ACTGACATTG CCGGTCAGGA TGTGGCCAAG CAGGCTCTAC AGGAAATGGT CATTCTACCC 
TCCGTCCGAC CGGAACTCTT TACGGGGCTT CGTGCACCGG CTAAGGGTTT ATTGCTGTTT 
GGTCCTCCCG GAAATGGCAA GACACTGCTG GCCCGCGCCG TGGCTACTGA GTGTAGTGCC 
ACCTTCCTGA ACATTTCGGC TGCCTCGCTG ACCAGCAAGT ACGTGGGGGA TGGCGAGAAA 
CTGGTGAGGG CTCTCTTCGC CGTGGCCCGT CACATGCAGC CCTCCATTAT TTTCATCGAC 
GAGGTGGACT CGTTGCTTTC AGAGCGAAGC AGCAGCGAGC ATGAAGCATC GCGTCGCCTA 
AAGACCGAGT TTCTAGTGGA GTTCGATGGG CTGCCTGGAA ATCCAGACGG AGACAGGATC 
GTGGTGCTGG CCGCCACCAA TCGACCGCAG GAATTGGACG AGGCAGCCCT GCGTCGGTTC 
ACAAAGCGCG TTTACGTCTC ACTGCCCGAC GAGCAAACCC GCGAGCTGCT CCTCAATCGA 
CTGCTCCAGA AGCAAGGCAG TCCGTTGGAT ACCGAGGCGT TGCGTCGCCT TGCAAAGATA 
ACAGACGGAT ACTCGGGCTC CGACTTAACG GCACTGGCCA AGGACGCCGC ATTGGAGCCC 
ATTCGAGAGT TGAATGTGGA GCAAGTGAAG TGTCTGGACA TCAGTGCAAT GCGTGCAATC 
ACAGAGCAGG ACTTTCACAG TTCGCTCAAG CGCATCAGGC GCTCGGTGGC GCCGCAAAGC 
CTCAACTCAT ATGAGAAGTG GTCGCAAGAT TATGGCGACA TCACCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spas ( NM_001316509.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303438.1 Drosophila melanogaster spastin (spas), transcript variant E, mRNA.

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CloneID	OFa41226
Clone ID Related Accession (Same CDS sequence)	NM_001316509.1
Accession Version	NM_001316509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spastin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCCA ATCGTCCGGG AGGAGGGTAT TCACCTGGTC CCGGCGATCC ATTGCTGGCC 
AAGCAGAAGC ATCACCATCG ACGCGCCTTC GAGTACATCT CCAAGGCGCT CAAAATTGAT 
GAGGAGAACG AAGGTCACAA AGAGCTGGCA ATTGAGCTCT ACCGAAAAGG TATCAAGGAG 
CTAGAGGATG GCATAGCTGT CGATTGCTGG AGTGGACGTG GTGATGTGTG GGATCGGGCT 
CAGCGCCTGC ACGACAAGAT GCAGACAAAC CTTTCGATGG CGCGGGATCG TCTCCATTTT 
CTAGCTCTGC GTGAGCAGGA TTTGCAAATG CAGCGCCTCT CCTTAAAGGA GAAGCAGAAA 
GAAGAGGCTC AAAGCAAGCC GCAGAAGACC AGGGAGCCCA TGCTGGCAGG AATGACCAAC 
GAACCAATGA AACTAAGGGT GCGCAGCAGT GGCTATGGGC CCAAGGCCAC CACCAGTGCC 
CAACCCACTG CATCTGGCCG CAAACTGACA ATTGGATCCA AACGACCCGT CAACTTGGCC 
GTGGCCAACA AATCACAAAC GCTGCCCCGC AACCTTGGCT CCAAAACATC TGTAGGCGCA 
GTCCAAAGGC AACCAGCCAA GACAGCTGCA ACGCCTCCCG CCGTTCGGCG ACAATTTTCT 
TCGGGGCGTA ATACGCCGCC CCAGCGTTCG CGGACTCCCA TAAACAACAA CGGACCCAGT 
GGCAGTGGCG CCAGCACTCC GGTGGTTAGT GTGAAGGGAG TGGAACAGAA GCTGGTGCAA 
CTTATACTCG ACGAGATCGT CGAGGGTGGG GCCAAAGTGG AGTGGACTGA CATTGCCGGT 
CAGGATGTGG CCAAGCAGGC TCTACAGGAA ATGGTCATTC TACCCTCCGT CCGACCGGAA 
CTCTTTACGG GGCTTCGTGC ACCGGCTAAG GGTTTATTGC TGTTTGGTCC TCCCGGAAAT 
GGCAAGACAC TGCTGGCCCG CGCCGTGGCT ACTGAGTGTA GTGCCACCTT CCTGAACATT 
TCGGCTGCCT CGCTGACCAG CAAGTACGTG GGGGATGGCG AGAAACTGGT GAGGGCTCTC 
TTCGCCGTGG CCCGTCACAT GCAGCCCTCC ATTATTTTCA TCGACGAGGT GGACTCGTTG 
CTTTCAGAGC GAAGCAGCAG CGAGCATGAA GCATCGCGTC GCCTAAAGAC CGAGTTTCTA 
GTGGAGTTCG ATGGGCTGCC TGGAAATCCA GACGGAGACA GGATCGTGGT GCTGGCCGCC 
ACCAATCGAC CGCAGGAATT GGACGAGGCA GCCCTGCGTC GGTTCACAAA GCGCGTTTAC 
GTCTCACTGC CCGACGAGCA AACCCGCGAG CTGCTCCTCA ATCGACTGCT CCAGAAGCAA 
GGCAGTCCGT TGGATACCGA GGCGTTGCGT CGCCTTGCAA AGATAACAGA CGGATACTCG 
GGCTCCGACT TAACGGCACT GGCCAAGGAC GCCGCATTGG AGCCCATTCG AGAGTTGAAT 
GTGGAGCAAG TGAAGTGTCT GGACATCAGT GCAATGCGTG CAATCACAGA GCAGGACTTT 
CACAGTTCGC TCAAGCGCAT CAGGCGCTCG GTGGCGCCGC AAAGCCTCAA CTCATATGAG 
AAGTGGTCGC AAGATTATGG CGACATCACC ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303438.1
CDS	916..2571
Translation	MAANRPGGGYSPGPGDPLLAKQKHHHRRAFEYISKALKIDEENEGHKELAIELYRKGIKELEDGIAVDCWSGRGDVWDRAQRLHDKMQTNLSMARDRLHFLALREQDLQMQRLSLKEKQKEEAQSKPQKTREPMLAGMTNEPMKLRVRSSGYGPKATTSAQPTASGRKLTIGSKRPVNLAVANKSQTLPRNLGSKTSVGAVQRQPAKTAATPPAVRRQFSSGRNTPPQRSRTPINNNGPSGSGASTPVVSVKGVEQKLVQLILDEIVEGGAKVEWTDIAGQDVAKQALQEMVILPSVRPELFTGLRAPAKGLLLFGPPGNGKTLLARAVATECSATFLNISAASLTSKYVGDGEKLVRALFAVARHMQPSIIFIDEVDSLLSERSSSEHEASRRLKTEFLVEFDGLPGNPDGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPDEQTRELLLNRLLQKQGSPLDTEALRRLAKITDGYSGSDLTALAKDAALEPIRELNVEQVKCLDISAMRAITEQDFHSSLKRIRRSVAPQSLNSYEKWSQDYGDITI

Target ORF information:

RefSeq Version	NM_001316509.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spastin (spas), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316509.1

ORF Insert Sequence:

ATGGCCGCCA ATCGTCCGGG AGGAGGGTAT TCACCTGGTC CCGGCGATCC ATTGCTGGCC 
AAGCAGAAGC ATCACCATCG ACGCGCCTTC GAGTACATCT CCAAGGCGCT CAAAATTGAT 
GAGGAGAACG AAGGTCACAA AGAGCTGGCA ATTGAGCTCT ACCGAAAAGG TATCAAGGAG 
CTAGAGGATG GCATAGCTGT CGATTGCTGG AGTGGACGTG GTGATGTGTG GGATCGGGCT 
CAGCGCCTGC ACGACAAGAT GCAGACAAAC CTTTCGATGG CGCGGGATCG TCTCCATTTT 
CTAGCTCTGC GTGAGCAGGA TTTGCAAATG CAGCGCCTCT CCTTAAAGGA GAAGCAGAAA 
GAAGAGGCTC AAAGCAAGCC GCAGAAGACC AGGGAGCCCA TGCTGGCAGG AATGACCAAC 
GAACCAATGA AACTAAGGGT GCGCAGCAGT GGCTATGGGC CCAAGGCCAC CACCAGTGCC 
CAACCCACTG CATCTGGCCG CAAACTGACA ATTGGATCCA AACGACCCGT CAACTTGGCC 
GTGGCCAACA AATCACAAAC GCTGCCCCGC AACCTTGGCT CCAAAACATC TGTAGGCGCA 
GTCCAAAGGC AACCAGCCAA GACAGCTGCA ACGCCTCCCG CCGTTCGGCG ACAATTTTCT 
TCGGGGCGTA ATACGCCGCC CCAGCGTTCG CGGACTCCCA TAAACAACAA CGGACCCAGT 
GGCAGTGGCG CCAGCACTCC GGTGGTTAGT GTGAAGGGAG TGGAACAGAA GCTGGTGCAA 
CTTATACTCG ACGAGATCGT CGAGGGTGGG GCCAAAGTGG AGTGGACTGA CATTGCCGGT 
CAGGATGTGG CCAAGCAGGC TCTACAGGAA ATGGTCATTC TACCCTCCGT CCGACCGGAA 
CTCTTTACGG GGCTTCGTGC ACCGGCTAAG GGTTTATTGC TGTTTGGTCC TCCCGGAAAT 
GGCAAGACAC TGCTGGCCCG CGCCGTGGCT ACTGAGTGTA GTGCCACCTT CCTGAACATT 
TCGGCTGCCT CGCTGACCAG CAAGTACGTG GGGGATGGCG AGAAACTGGT GAGGGCTCTC 
TTCGCCGTGG CCCGTCACAT GCAGCCCTCC ATTATTTTCA TCGACGAGGT GGACTCGTTG 
CTTTCAGAGC GAAGCAGCAG CGAGCATGAA GCATCGCGTC GCCTAAAGAC CGAGTTTCTA 
GTGGAGTTCG ATGGGCTGCC TGGAAATCCA GACGGAGACA GGATCGTGGT GCTGGCCGCC 
ACCAATCGAC CGCAGGAATT GGACGAGGCA GCCCTGCGTC GGTTCACAAA GCGCGTTTAC 
GTCTCACTGC CCGACGAGCA AACCCGCGAG CTGCTCCTCA ATCGACTGCT CCAGAAGCAA 
GGCAGTCCGT TGGATACCGA GGCGTTGCGT CGCCTTGCAA AGATAACAGA CGGATACTCG 
GGCTCCGACT TAACGGCACT GGCCAAGGAC GCCGCATTGG AGCCCATTCG AGAGTTGAAT 
GTGGAGCAAG TGAAGTGTCT GGACATCAGT GCAATGCGTG CAATCACAGA GCAGGACTTT 
CACAGTTCGC TCAAGCGCAT CAGGCGCTCG GTGGCGCCGC AAAGCCTCAA CTCATATGAG 
AAGTGGTCGC AAGATTATGG CGACATCACC ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spas cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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