Hrd3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hrd3
Entrez Gene ID	42806
Full Name	HMG-coA reductase degradation 3
Synonyms	BcDNA:LD23587,CG10221,Dmel\CG10221,HRD3
General protein information	Preferred Names HMG-coA reductase degradation 3 Names CG10221 gene product from transcript CG10221-RB CG10221-PA CG10221-PB Hrd3-PA Hrd3-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95B1-95B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275953.1	NP_001262882.1	HMG-coA reductase degradation 3, isoform B
NM_142922.3	NP_651179.1	HMG-coA reductase degradation 3, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	SEL1L	NP_005056.3
Arabidopsis thaliana (thale cress)	AT1G18260	NP_564049.1
Pan troglodytes (chimpanzee)	SEL1L	XP_510102.3
Bos taurus (cattle)	SEL1L	NP_001193837.1
Gallus gallus (chicken)	SEL1L	XP_421303.2
Danio rerio (zebrafish)	sel1l	NP_001038629.1
Caenorhabditis elegans (roundworm)	sel-1	NP_506144.1
Rattus norvegicus (Norway rat)	Sel1l	NP_808794.2
Mus musculus (house mouse)	Sel1l	NP_001034178.1
Drosophila melanogaster (fruit fly)	Hrd3	NP_001262882.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000615	XP_310466.4
Arabidopsis thaliana (thale cress)	AT1G73570	NP_177498.1
Xenopus tropicalis (tropical clawed frog)	sel1l	XP_002938416.1
	SEL1L	XP_001105925.1
Canis lupus familiaris (dog)	SEL1L	XP_537530.2
Oryza sativa (rice)	Os03g0259300	NP_001049611.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hrd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hrd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05827	NM_001275953.1 Latest version!	Drosophila melanogaster HMG-coA reductase degradation 3 (Hrd3), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa05827	NM_142922.3 Latest version!	Drosophila melanogaster HMG-coA reductase degradation 3 (Hrd3), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hrd3 ( NM_001275953.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262882.1 Drosophila melanogaster HMG-coA reductase degradation 3 (Hrd3), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05827
Clone ID Related Accession (Same CDS sequence)	NM_001275953.1 , NM_142922.3
Accession Version	NM_001275953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	HMG-coA reductase degradation 3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGCCA ACAGCTGGAT GTGCCTGCTG GCGCTGCTTC TCGCCTGGCA GCTGCGAGCG 
GAGGAGCTCC AAGGGCCCAA CATTGATGGT ACGGGCAGCA GTTTGGGTTC GGCCACAGGT 
ACGGGTACGG GAACGGGATT GGGCACGGGA ACAGCCACGC CAACGCTGCC CAATGAGCCC 
TCCACCCAGG CGGAGGCGGA GGAGGTGACC ACGGCGGAAT CAGAGTCGCG GGTTCCGTCG 
GAAAGCAACG AACGCAAGGC AGACAAACCC AAGGAGTCCA CAAAGTTTGC TTCCAGGACG 
AAGGTTATGC CGGCTTCCCT GTTCGAACGC CTGTGGAACG AAAGTCTGAT CAGGCGGCAA 
AAGCTGAAAG CCGAGATCGA GCGCATCGAA GCCAAACGCA AATCCACTGA AGTGTTAGAA 
CTTACCCCAG ACCAAATAGA AGCTCAAACC GTGTACGACA ACGCAATGGA CATGTTGGCC 
AAGCCAAGCA CGGATAAGCG AGTAGCCATG ATTTTGCTTA AGAAGGCTGC TGCATTTAAT 
CACCTCAAGG CGAGGGCGGA ACTAGCCTGG GCCGCCCTAC TGGGTCAATG GATGGACTTT 
GATTTCAACC ATGCTGTCGA TGAGTTTGAG AGTCTGGCCA ACGAAGGCGT CCCAGAGGCT 
CACATGGGCC TGGGATTCCT TTATTCGGTG GGCGTTGGCG GTAAAAATGT AAGCCAGCCC 
CTGGCACTGA TCCACTATAG CCTGGCTGCC CTCGGTGATA ATACTCCGGC GCAAATGGCC 
ATGGGGTATC GCTATCTTTA TGGCATCAAT GTGCCCATTA GCTGTGAGAA GGCGCTGATC 
CACTACAAGC GAGTAGCCAA AAAAGTGGCT TCCAAGATAA CCTTCGCCAA TGGGCCGGTT 
GTGCATCGGG TAAGACTGCT GGATGACCTA GAGAATCCCG GCAGTTCCGA AACTGAGATT 
GTCGACTATT ACAAACTTCT GGCCGATAAA GGCGACATCC AGTCGCAGGT GGGATTGGGT 
CAGGTGTACT ATCAGGGCGG CAAGGTCACC GAGCAAGATC ATCAAAAGGC TTTGGAATAT 
TTCAAAATGG CCGCCGATGC AGGTAGTGCA GTTGGCATCG CGTTTTTAGG CAAGCTTTAT 
CTGGAAGGAA GTGATCAAAT CAGGGCCGAT AATGATGCAG CATTTGGGTA CTTCTCCAAG 
GCCGCTGAAA TGGGGGATCC AGTTGGTCAA AGCGGACTGG GCCTCATGTA TCTAAAGGGT 
TTAGGTGTTC CCAAAGACTC CATCAAGGCA TTATCCTATT TCACTCAGGC GGCTGATCAA 
GGTTGGGTTG ATGGGCAACT GCAGCTGGGC AACATGTATT TCACTGGCAA TGGTGTAAAA 
ACCGACTACA AGTTAGCCTT TAAGTACTTC AACCTGGCCA CCCAATCAGG CCATGTTTTA 
GCCTATTATA ATCTGGGAGT GATGAATGCC TACGGTATGG GAATGCTGCG TTCATGTCCA 
GCAGCAGTAG AATTCTTCAA GACTGTTTCG GAGCGTGGCC GTTGGAGCAG CCGATTGATG 
ATGGCCTACA GCGACTATAA AAATAACCGC ATCGACGAGG CCTACATGCA GTACTCGTTG 
ATGGCCGAAA TGGGCTATGA GGTGGCTCAA AGTAATGCCG CCTTTTTACT AGACCGCAAG 
GAGGTGCATG TGTTCAACGA TCGGCACGAA GATCTGATTC GTGCCTTCTA CTACTGGAAA 
CGAGCAGCCG GACAGGGCTA TTCTGCGGCC CAAGTCAAGC TGGGCGATTA CTATTACTAC 
GGTTGGGGTA CTTCGACGGA CTTCGAGACT GCCGCTGCTC TTTACAGGAA AGCCTCTGAG 
CAGCAATATA ATGCCCAAGC CATGTTCAAC TTGGGATACA TGCACGAGCA AGGGCTCGGA 
ATGAAGAAAG ACTGGCATCT GGCAAAGAGA TTATACGATT TGGCTGCGGA AACCAATTCA 
GACGCCAAAG TGCCTGTGGC CATTGCGCTT TTTAAGCTAC AAATGTTGGC CACGATTGAA 
TCAATCAAGG AGTCACCTTA CAGGTTCATC TTCTACATGG ATGAAAATAT TGCTGCCAAT 
TGGGACTTGT ACATGATTAC CGTTCTAACT CTACTGCTGG GCATCATCAT GTACATGAGG 
CGTCCATTTC AGCAGCCGGA TCAGCCGGCA CAGCAGCCAG CTGCAGATGA CATTGCTGCT 
GCGCCAGTTA ACTTGGCGCC GGTTGGAGCG GAAGCAGCAC CTGTAGCCGC AGTGGTCGAA 
GGTGTGGGCG TCACACCACC TGCCGCCGCA GTTTCATCTC ACGATGCTGT GGCCGCGTCC 
TCCTCTACGG AAACCTCATC AACCACTGCC ACGTCCCTTG TCGATGCAGC GTCACAAGGG 
GGAAGTGGCA CGACATCTGC GGCGCCGGAT GGCGCTAACA CACTCGATCC CACTGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262882.1
CDS	644..3103
Translation	MKANSWMCLLALLLAWQLRAEELQGPNIDGTGSSLGSATGTGTGTGLGTGTATPTLPNEPSTQAEAEEVTTAESESRVPSESNERKADKPKESTKFASRTKVMPASLFERLWNESLIRRQKLKAEIERIEAKRKSTEVLELTPDQIEAQTVYDNAMDMLAKPSTDKRVAMILLKKAAAFNHLKARAELAWAALLGQWMDFDFNHAVDEFESLANEGVPEAHMGLGFLYSVGVGGKNVSQPLALIHYSLAALGDNTPAQMAMGYRYLYGINVPISCEKALIHYKRVAKKVASKITFANGPVVHRVRLLDDLENPGSSETEIVDYYKLLADKGDIQSQVGLGQVYYQGGKVTEQDHQKALEYFKMAADAGSAVGIAFLGKLYLEGSDQIRADNDAAFGYFSKAAEMGDPVGQSGLGLMYLKGLGVPKDSIKALSYFTQAADQGWVDGQLQLGNMYFTGNGVKTDYKLAFKYFNLATQSGHVLAYYNLGVMNAYGMGMLRSCPAAVEFFKTVSERGRWSSRLMMAYSDYKNNRIDEAYMQYSLMAEMGYEVAQSNAAFLLDRKEVHVFNDRHEDLIRAFYYWKRAAGQGYSAAQVKLGDYYYYGWGTSTDFETAAALYRKASEQQYNAQAMFNLGYMHEQGLGMKKDWHLAKRLYDLAAETNSDAKVPVAIALFKLQMLATIESIKESPYRFIFYMDENIAANWDLYMITVLTLLLGIIMYMRRPFQQPDQPAQQPAADDIAAAPVNLAPVGAEAAPVAAVVEGVGVTPPAAAVSSHDAVAASSSTETSSTTATSLVDAASQGGSGTTSAAPDGANTLDPTG

Target ORF information:

RefSeq Version	NM_001275953.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster HMG-coA reductase degradation 3 (Hrd3), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275953.1

ORF Insert Sequence:

ATGAAGGCCA ACAGCTGGAT GTGCCTGCTG GCGCTGCTTC TCGCCTGGCA GCTGCGAGCG 
GAGGAGCTCC AAGGGCCCAA CATTGATGGT ACGGGCAGCA GTTTGGGTTC GGCCACAGGT 
ACGGGTACGG GAACGGGATT GGGCACGGGA ACAGCCACGC CAACGCTGCC CAATGAGCCC 
TCCACCCAGG CGGAGGCGGA GGAGGTGACC ACGGCGGAAT CAGAGTCGCG GGTTCCGTCG 
GAAAGCAACG AACGCAAGGC AGACAAACCC AAGGAGTCCA CAAAGTTTGC TTCCAGGACG 
AAGGTTATGC CGGCTTCCCT GTTCGAACGC CTGTGGAACG AAAGTCTGAT CAGGCGGCAA 
AAGCTGAAAG CCGAGATCGA GCGCATCGAA GCCAAACGCA AATCCACTGA AGTGTTAGAA 
CTTACCCCAG ACCAAATAGA AGCTCAAACC GTGTACGACA ACGCAATGGA CATGTTGGCC 
AAGCCAAGCA CGGATAAGCG AGTAGCCATG ATTTTGCTTA AGAAGGCTGC TGCATTTAAT 
CACCTCAAGG CGAGGGCGGA ACTAGCCTGG GCCGCCCTAC TGGGTCAATG GATGGACTTT 
GATTTCAACC ATGCTGTCGA TGAGTTTGAG AGTCTGGCCA ACGAAGGCGT CCCAGAGGCT 
CACATGGGCC TGGGATTCCT TTATTCGGTG GGCGTTGGCG GTAAAAATGT AAGCCAGCCC 
CTGGCACTGA TCCACTATAG CCTGGCTGCC CTCGGTGATA ATACTCCGGC GCAAATGGCC 
ATGGGGTATC GCTATCTTTA TGGCATCAAT GTGCCCATTA GCTGTGAGAA GGCGCTGATC 
CACTACAAGC GAGTAGCCAA AAAAGTGGCT TCCAAGATAA CCTTCGCCAA TGGGCCGGTT 
GTGCATCGGG TAAGACTGCT GGATGACCTA GAGAATCCCG GCAGTTCCGA AACTGAGATT 
GTCGACTATT ACAAACTTCT GGCCGATAAA GGCGACATCC AGTCGCAGGT GGGATTGGGT 
CAGGTGTACT ATCAGGGCGG CAAGGTCACC GAGCAAGATC ATCAAAAGGC TTTGGAATAT 
TTCAAAATGG CCGCCGATGC AGGTAGTGCA GTTGGCATCG CGTTTTTAGG CAAGCTTTAT 
CTGGAAGGAA GTGATCAAAT CAGGGCCGAT AATGATGCAG CATTTGGGTA CTTCTCCAAG 
GCCGCTGAAA TGGGGGATCC AGTTGGTCAA AGCGGACTGG GCCTCATGTA TCTAAAGGGT 
TTAGGTGTTC CCAAAGACTC CATCAAGGCA TTATCCTATT TCACTCAGGC GGCTGATCAA 
GGTTGGGTTG ATGGGCAACT GCAGCTGGGC AACATGTATT TCACTGGCAA TGGTGTAAAA 
ACCGACTACA AGTTAGCCTT TAAGTACTTC AACCTGGCCA CCCAATCAGG CCATGTTTTA 
GCCTATTATA ATCTGGGAGT GATGAATGCC TACGGTATGG GAATGCTGCG TTCATGTCCA 
GCAGCAGTAG AATTCTTCAA GACTGTTTCG GAGCGTGGCC GTTGGAGCAG CCGATTGATG 
ATGGCCTACA GCGACTATAA AAATAACCGC ATCGACGAGG CCTACATGCA GTACTCGTTG 
ATGGCCGAAA TGGGCTATGA GGTGGCTCAA AGTAATGCCG CCTTTTTACT AGACCGCAAG 
GAGGTGCATG TGTTCAACGA TCGGCACGAA GATCTGATTC GTGCCTTCTA CTACTGGAAA 
CGAGCAGCCG GACAGGGCTA TTCTGCGGCC CAAGTCAAGC TGGGCGATTA CTATTACTAC 
GGTTGGGGTA CTTCGACGGA CTTCGAGACT GCCGCTGCTC TTTACAGGAA AGCCTCTGAG 
CAGCAATATA ATGCCCAAGC CATGTTCAAC TTGGGATACA TGCACGAGCA AGGGCTCGGA 
ATGAAGAAAG ACTGGCATCT GGCAAAGAGA TTATACGATT TGGCTGCGGA AACCAATTCA 
GACGCCAAAG TGCCTGTGGC CATTGCGCTT TTTAAGCTAC AAATGTTGGC CACGATTGAA 
TCAATCAAGG AGTCACCTTA CAGGTTCATC TTCTACATGG ATGAAAATAT TGCTGCCAAT 
TGGGACTTGT ACATGATTAC CGTTCTAACT CTACTGCTGG GCATCATCAT GTACATGAGG 
CGTCCATTTC AGCAGCCGGA TCAGCCGGCA CAGCAGCCAG CTGCAGATGA CATTGCTGCT 
GCGCCAGTTA ACTTGGCGCC GGTTGGAGCG GAAGCAGCAC CTGTAGCCGC AGTGGTCGAA 
GGTGTGGGCG TCACACCACC TGCCGCCGCA GTTTCATCTC ACGATGCTGT GGCCGCGTCC 
TCCTCTACGG AAACCTCATC AACCACTGCC ACGTCCCTTG TCGATGCAGC GTCACAAGGG 
GGAAGTGGCA CGACATCTGC GGCGCCGGAT GGCGCTAACA CACTCGATCC CACTGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hrd3 ( NM_142922.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651179.1 Drosophila melanogaster HMG-coA reductase degradation 3 (Hrd3), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05827
Clone ID Related Accession (Same CDS sequence)	NM_001275953.1 , NM_142922.3
Accession Version	NM_142922.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	HMG-coA reductase degradation 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142922.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGCCA ACAGCTGGAT GTGCCTGCTG GCGCTGCTTC TCGCCTGGCA GCTGCGAGCG 
GAGGAGCTCC AAGGGCCCAA CATTGATGGT ACGGGCAGCA GTTTGGGTTC GGCCACAGGT 
ACGGGTACGG GAACGGGATT GGGCACGGGA ACAGCCACGC CAACGCTGCC CAATGAGCCC 
TCCACCCAGG CGGAGGCGGA GGAGGTGACC ACGGCGGAAT CAGAGTCGCG GGTTCCGTCG 
GAAAGCAACG AACGCAAGGC AGACAAACCC AAGGAGTCCA CAAAGTTTGC TTCCAGGACG 
AAGGTTATGC CGGCTTCCCT GTTCGAACGC CTGTGGAACG AAAGTCTGAT CAGGCGGCAA 
AAGCTGAAAG CCGAGATCGA GCGCATCGAA GCCAAACGCA AATCCACTGA AGTGTTAGAA 
CTTACCCCAG ACCAAATAGA AGCTCAAACC GTGTACGACA ACGCAATGGA CATGTTGGCC 
AAGCCAAGCA CGGATAAGCG AGTAGCCATG ATTTTGCTTA AGAAGGCTGC TGCATTTAAT 
CACCTCAAGG CGAGGGCGGA ACTAGCCTGG GCCGCCCTAC TGGGTCAATG GATGGACTTT 
GATTTCAACC ATGCTGTCGA TGAGTTTGAG AGTCTGGCCA ACGAAGGCGT CCCAGAGGCT 
CACATGGGCC TGGGATTCCT TTATTCGGTG GGCGTTGGCG GTAAAAATGT AAGCCAGCCC 
CTGGCACTGA TCCACTATAG CCTGGCTGCC CTCGGTGATA ATACTCCGGC GCAAATGGCC 
ATGGGGTATC GCTATCTTTA TGGCATCAAT GTGCCCATTA GCTGTGAGAA GGCGCTGATC 
CACTACAAGC GAGTAGCCAA AAAAGTGGCT TCCAAGATAA CCTTCGCCAA TGGGCCGGTT 
GTGCATCGGG TAAGACTGCT GGATGACCTA GAGAATCCCG GCAGTTCCGA AACTGAGATT 
GTCGACTATT ACAAACTTCT GGCCGATAAA GGCGACATCC AGTCGCAGGT GGGATTGGGT 
CAGGTGTACT ATCAGGGCGG CAAGGTCACC GAGCAAGATC ATCAAAAGGC TTTGGAATAT 
TTCAAAATGG CCGCCGATGC AGGTAGTGCA GTTGGCATCG CGTTTTTAGG CAAGCTTTAT 
CTGGAAGGAA GTGATCAAAT CAGGGCCGAT AATGATGCAG CATTTGGGTA CTTCTCCAAG 
GCCGCTGAAA TGGGGGATCC AGTTGGTCAA AGCGGACTGG GCCTCATGTA TCTAAAGGGT 
TTAGGTGTTC CCAAAGACTC CATCAAGGCA TTATCCTATT TCACTCAGGC GGCTGATCAA 
GGTTGGGTTG ATGGGCAACT GCAGCTGGGC AACATGTATT TCACTGGCAA TGGTGTAAAA 
ACCGACTACA AGTTAGCCTT TAAGTACTTC AACCTGGCCA CCCAATCAGG CCATGTTTTA 
GCCTATTATA ATCTGGGAGT GATGAATGCC TACGGTATGG GAATGCTGCG TTCATGTCCA 
GCAGCAGTAG AATTCTTCAA GACTGTTTCG GAGCGTGGCC GTTGGAGCAG CCGATTGATG 
ATGGCCTACA GCGACTATAA AAATAACCGC ATCGACGAGG CCTACATGCA GTACTCGTTG 
ATGGCCGAAA TGGGCTATGA GGTGGCTCAA AGTAATGCCG CCTTTTTACT AGACCGCAAG 
GAGGTGCATG TGTTCAACGA TCGGCACGAA GATCTGATTC GTGCCTTCTA CTACTGGAAA 
CGAGCAGCCG GACAGGGCTA TTCTGCGGCC CAAGTCAAGC TGGGCGATTA CTATTACTAC 
GGTTGGGGTA CTTCGACGGA CTTCGAGACT GCCGCTGCTC TTTACAGGAA AGCCTCTGAG 
CAGCAATATA ATGCCCAAGC CATGTTCAAC TTGGGATACA TGCACGAGCA AGGGCTCGGA 
ATGAAGAAAG ACTGGCATCT GGCAAAGAGA TTATACGATT TGGCTGCGGA AACCAATTCA 
GACGCCAAAG TGCCTGTGGC CATTGCGCTT TTTAAGCTAC AAATGTTGGC CACGATTGAA 
TCAATCAAGG AGTCACCTTA CAGGTTCATC TTCTACATGG ATGAAAATAT TGCTGCCAAT 
TGGGACTTGT ACATGATTAC CGTTCTAACT CTACTGCTGG GCATCATCAT GTACATGAGG 
CGTCCATTTC AGCAGCCGGA TCAGCCGGCA CAGCAGCCAG CTGCAGATGA CATTGCTGCT 
GCGCCAGTTA ACTTGGCGCC GGTTGGAGCG GAAGCAGCAC CTGTAGCCGC AGTGGTCGAA 
GGTGTGGGCG TCACACCACC TGCCGCCGCA GTTTCATCTC ACGATGCTGT GGCCGCGTCC 
TCCTCTACGG AAACCTCATC AACCACTGCC ACGTCCCTTG TCGATGCAGC GTCACAAGGG 
GGAAGTGGCA CGACATCTGC GGCGCCGGAT GGCGCTAACA CACTCGATCC CACTGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651179.1
CDS	380..2839
Translation	MKANSWMCLLALLLAWQLRAEELQGPNIDGTGSSLGSATGTGTGTGLGTGTATPTLPNEPSTQAEAEEVTTAESESRVPSESNERKADKPKESTKFASRTKVMPASLFERLWNESLIRRQKLKAEIERIEAKRKSTEVLELTPDQIEAQTVYDNAMDMLAKPSTDKRVAMILLKKAAAFNHLKARAELAWAALLGQWMDFDFNHAVDEFESLANEGVPEAHMGLGFLYSVGVGGKNVSQPLALIHYSLAALGDNTPAQMAMGYRYLYGINVPISCEKALIHYKRVAKKVASKITFANGPVVHRVRLLDDLENPGSSETEIVDYYKLLADKGDIQSQVGLGQVYYQGGKVTEQDHQKALEYFKMAADAGSAVGIAFLGKLYLEGSDQIRADNDAAFGYFSKAAEMGDPVGQSGLGLMYLKGLGVPKDSIKALSYFTQAADQGWVDGQLQLGNMYFTGNGVKTDYKLAFKYFNLATQSGHVLAYYNLGVMNAYGMGMLRSCPAAVEFFKTVSERGRWSSRLMMAYSDYKNNRIDEAYMQYSLMAEMGYEVAQSNAAFLLDRKEVHVFNDRHEDLIRAFYYWKRAAGQGYSAAQVKLGDYYYYGWGTSTDFETAAALYRKASEQQYNAQAMFNLGYMHEQGLGMKKDWHLAKRLYDLAAETNSDAKVPVAIALFKLQMLATIESIKESPYRFIFYMDENIAANWDLYMITVLTLLLGIIMYMRRPFQQPDQPAQQPAADDIAAAPVNLAPVGAEAAPVAAVVEGVGVTPPAAAVSSHDAVAASSSTETSSTTATSLVDAASQGGSGTTSAAPDGANTLDPTG

Target ORF information:

RefSeq Version	NM_142922.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster HMG-coA reductase degradation 3 (Hrd3), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142922.3

ORF Insert Sequence:

ATGAAGGCCA ACAGCTGGAT GTGCCTGCTG GCGCTGCTTC TCGCCTGGCA GCTGCGAGCG 
GAGGAGCTCC AAGGGCCCAA CATTGATGGT ACGGGCAGCA GTTTGGGTTC GGCCACAGGT 
ACGGGTACGG GAACGGGATT GGGCACGGGA ACAGCCACGC CAACGCTGCC CAATGAGCCC 
TCCACCCAGG CGGAGGCGGA GGAGGTGACC ACGGCGGAAT CAGAGTCGCG GGTTCCGTCG 
GAAAGCAACG AACGCAAGGC AGACAAACCC AAGGAGTCCA CAAAGTTTGC TTCCAGGACG 
AAGGTTATGC CGGCTTCCCT GTTCGAACGC CTGTGGAACG AAAGTCTGAT CAGGCGGCAA 
AAGCTGAAAG CCGAGATCGA GCGCATCGAA GCCAAACGCA AATCCACTGA AGTGTTAGAA 
CTTACCCCAG ACCAAATAGA AGCTCAAACC GTGTACGACA ACGCAATGGA CATGTTGGCC 
AAGCCAAGCA CGGATAAGCG AGTAGCCATG ATTTTGCTTA AGAAGGCTGC TGCATTTAAT 
CACCTCAAGG CGAGGGCGGA ACTAGCCTGG GCCGCCCTAC TGGGTCAATG GATGGACTTT 
GATTTCAACC ATGCTGTCGA TGAGTTTGAG AGTCTGGCCA ACGAAGGCGT CCCAGAGGCT 
CACATGGGCC TGGGATTCCT TTATTCGGTG GGCGTTGGCG GTAAAAATGT AAGCCAGCCC 
CTGGCACTGA TCCACTATAG CCTGGCTGCC CTCGGTGATA ATACTCCGGC GCAAATGGCC 
ATGGGGTATC GCTATCTTTA TGGCATCAAT GTGCCCATTA GCTGTGAGAA GGCGCTGATC 
CACTACAAGC GAGTAGCCAA AAAAGTGGCT TCCAAGATAA CCTTCGCCAA TGGGCCGGTT 
GTGCATCGGG TAAGACTGCT GGATGACCTA GAGAATCCCG GCAGTTCCGA AACTGAGATT 
GTCGACTATT ACAAACTTCT GGCCGATAAA GGCGACATCC AGTCGCAGGT GGGATTGGGT 
CAGGTGTACT ATCAGGGCGG CAAGGTCACC GAGCAAGATC ATCAAAAGGC TTTGGAATAT 
TTCAAAATGG CCGCCGATGC AGGTAGTGCA GTTGGCATCG CGTTTTTAGG CAAGCTTTAT 
CTGGAAGGAA GTGATCAAAT CAGGGCCGAT AATGATGCAG CATTTGGGTA CTTCTCCAAG 
GCCGCTGAAA TGGGGGATCC AGTTGGTCAA AGCGGACTGG GCCTCATGTA TCTAAAGGGT 
TTAGGTGTTC CCAAAGACTC CATCAAGGCA TTATCCTATT TCACTCAGGC GGCTGATCAA 
GGTTGGGTTG ATGGGCAACT GCAGCTGGGC AACATGTATT TCACTGGCAA TGGTGTAAAA 
ACCGACTACA AGTTAGCCTT TAAGTACTTC AACCTGGCCA CCCAATCAGG CCATGTTTTA 
GCCTATTATA ATCTGGGAGT GATGAATGCC TACGGTATGG GAATGCTGCG TTCATGTCCA 
GCAGCAGTAG AATTCTTCAA GACTGTTTCG GAGCGTGGCC GTTGGAGCAG CCGATTGATG 
ATGGCCTACA GCGACTATAA AAATAACCGC ATCGACGAGG CCTACATGCA GTACTCGTTG 
ATGGCCGAAA TGGGCTATGA GGTGGCTCAA AGTAATGCCG CCTTTTTACT AGACCGCAAG 
GAGGTGCATG TGTTCAACGA TCGGCACGAA GATCTGATTC GTGCCTTCTA CTACTGGAAA 
CGAGCAGCCG GACAGGGCTA TTCTGCGGCC CAAGTCAAGC TGGGCGATTA CTATTACTAC 
GGTTGGGGTA CTTCGACGGA CTTCGAGACT GCCGCTGCTC TTTACAGGAA AGCCTCTGAG 
CAGCAATATA ATGCCCAAGC CATGTTCAAC TTGGGATACA TGCACGAGCA AGGGCTCGGA 
ATGAAGAAAG ACTGGCATCT GGCAAAGAGA TTATACGATT TGGCTGCGGA AACCAATTCA 
GACGCCAAAG TGCCTGTGGC CATTGCGCTT TTTAAGCTAC AAATGTTGGC CACGATTGAA 
TCAATCAAGG AGTCACCTTA CAGGTTCATC TTCTACATGG ATGAAAATAT TGCTGCCAAT 
TGGGACTTGT ACATGATTAC CGTTCTAACT CTACTGCTGG GCATCATCAT GTACATGAGG 
CGTCCATTTC AGCAGCCGGA TCAGCCGGCA CAGCAGCCAG CTGCAGATGA CATTGCTGCT 
GCGCCAGTTA ACTTGGCGCC GGTTGGAGCG GAAGCAGCAC CTGTAGCCGC AGTGGTCGAA 
GGTGTGGGCG TCACACCACC TGCCGCCGCA GTTTCATCTC ACGATGCTGT GGCCGCGTCC 
TCCTCTACGG AAACCTCATC AACCACTGCC ACGTCCCTTG TCGATGCAGC GTCACAAGGG 
GGAAGTGGCA CGACATCTGC GGCGCCGGAT GGCGCTAACA CACTCGATCC CACTGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hrd3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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