DNApol-epsilon255 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNApol-epsilon255
Entrez Gene ID	42758
Full Name	DNA polymerase epsilon 255kD subunit
Synonyms	6768,BAB17608,CG6768,DNA pol epsilon,DNApol-epsilon,Dmel\CG6768,dpolepsilon,dpolepsilonp255,l(3)pl10,l(3)pl10R,pl[10],pol-epsilon
General protein information	Preferred Names DNA polymerase epsilon 255kD subunit Names CG6768 gene product from transcript CG6768-RA CG6768-PA DNA polymerase epsilon DNApol-epsilon DNApol-epsilon255-PA DNApolymarase E lethal (3) pl10
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94E13-94E13

mRNA and Protein(s)

mRNA	Protein	Name
NM_079738.3	NP_524462.2	DNA polymerase epsilon 255kD subunit

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

N-terminal catalytic domain of the DNA polymerase epsilon p255 subunit plays role in the G1-S transition and/or S-phase progression of the mitotic cycle.
Title: Differential requirement for the N-terminal catalytic domain of the DNA polymerase ? p255 subunit in the mitotic cell cycle and the endocycle.

The following DNApol-epsilon255 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNApol-epsilon255 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05782	NM_079738.3 Latest version!	Drosophila melanogaster DNA polymerase epsilon 255kD subunit (DNApol-epsilon255), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNApol-epsilon255 ( NM_079738.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524462.2 Drosophila melanogaster DNA polymerase epsilon 255kD subunit (DNApol-epsilon255), mRNA.

Price & Availability↑

CloneID	OFa05782
Clone ID Related Accession (Same CDS sequence)	NM_079738.3
Accession Version	NM_079738.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	DNA polymerase epsilon 255kD subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079738.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGACT CCAGCAAAGG CAAAGTGCTG CAAAATACGG GTAAATTCGT CAGCGAGAAT 
CGCACAGAAG GCGACGACTT CTTCAATGAG GCGGGCTATC GTCAATCCCG GGAGAACGAT 
AAAATCGACT CGAAATATGG CTTCGATCGG GTTAAGGACA GCCAGGAGCG GACGGGCTAC 
CTCATCAACA TGCATTCGAA CGAAGTTTTG GATGAGGACA GAAGATTGAT TGCTGCCTTG 
GACCTGTTCT TCATCCAAAT GGATGGTTCC CGCTTCAAAT GCACGGTGGC CTATCAGCCA 
TATTTACTCA TCCGACCAGA GGATAATATG CATCTGGAAG TGGCGCGATT TCTGGGTCGC 
AAGTATTCCG GCCAGATTTC TGGACTGGAG CACATAACCA AAGAAGATTT GGATCTGCCC 
AATCATCTAT CCGGTTTGCA GCAGCAGTAC ATAAAACTTT CGTTTCTCAA TCAGACAGCC 
ATGACCAAGG TTAGAAGGGA ACTCATGTCC GCGGTGAAGA GAAATCAGGA GCGACAGAAA 
TCCAACACAT ACTACATGCA AATGCTGGCC ACCTCGCTGG CCCAATCCTC CGCAGGTTCC 
GAGGATGCCA CATTGGGTAA GAGGCAGCAG GATTACATGG ATTGTATTGT GGACATAAGG 
GAGCATGATG TGCCTTACCA CGTCAGAGTG TCCATCGATT TGCGCATCTT TTGTGGACAG 
TGGTACAATA TCAGGTGCAG AAGTGGCGTG GAATTGCCTA CGATCACCTG CCGACCGGAT 
ATTCTGGACA GACCCGAGCC CGTGGTCCTG GCCTTTGATA TAGAAACCAC TAAGCTGCCC 
CTTAAGTTTC CCGATGCCCA GACGGATCAG GTTATGATGA TCTCGTACAT GATCGATGGT 
CAGGGTTATC TGATAACCAA TCGTGAGATT ATATCATCCA ATGTGGACGA TTTTGAGTAC 
ACTCCCAAGC CGGAATTCGA GGGTAACTTT ATAGTATTCA ACGAAGAGAA CGAGATGCAG 
CTGCTCCAGC GCTTCTTCGA TCACATCATG GAGGTGCGTC CCCACATCAT TGTTACATAC 
AACGGCGACT TCTTCGATTG GCCCTTCGTG GAGACGCGTG CTGCAGTGTA CGATCTGGAC 
ATGAAGCAAG AGATTGGCTT CTCCAAGCTA CGGGATGGCA ATTATCTAAG TCGCCCTGCC 
ATACACATGG ATTGCCTATG TTGGGTGAAA CGAGATTCTT ATTTACCTGT TGGATCTCAA 
GGCTTAAAGG CGGTGGCCAA GGCTAAATTA CGCTATGATC CTGTGGAACT CGATCCGGAG 
GATATGTGCC GCATGGCCGT GGAACAGCCC CAAGTGCTGG CCAATTACTC TGTATCCGAT 
GCGGTGGCCA CATACTATCT GTACATGAAG TATGTGCATC CATTTATCTT CGCCCTAAAT 
ACGATTATTC CCATGGAACC CGATGAGATC CTAAGAAAGG GTTCCGGCAC ACTCTGTGAA 
ACGTTGCTGA TGGTGGAGGC TTACCATGCC CAGATTGTGT ATCCCAACAA GCATCAGAGT 
GAGCTGAATA AGCTCTCCAA CGAGGGACAC GTACTGGATT CGGAAACCTA TGTGGGTGGT 
CATGTGGAGG CTTTGGAATC GGGTGTTTTC CGGGCGGACA TACCATGCCG TTTTCGTCTA 
GATCCTGCTA TGGTCAAGCA ACTGCAGGAG CAGGTTGATG CAGTTCTGCG CCACGCTATC 
GAAGTGGAGG AAGGCATACC GCTCGAGAAG GTCTTGAATC TGGATGAAGT GCGGCAGGAG 
ATTGTGCAGG GGCTACAGGG TCTGCACGAT ATACCCAATC GCTTGGAGCA GCCGGTCATC 
TATCACTTGG ATGTGGGTGC CATGTACCCC AACATTATTT TGACCAATCG CCTGCAGCCC 
TCGGCAATGG TTAGTGACTT AGATTGTGCC GCCTGTGACT TCAACAAGCC AGGAGTTCGG 
TGCAAACGTT CCATGGACTG GTTGTGGCGC GGCGAGATGT TGCCCGCCTC CAGGAACGAG 
TTTCAGCGCA TTCAGCAGCA GCTGGAGACC GAGAAGTTTC CACCCCTTTT CCCTGGCGGA 
CCACAGCGAG CCTTTCACGA GCTCTCCAAG GAGGATCAGG CGGCGTACGA GAAGAAACGT 
CTGACGGATT ACTGCCGCAA GGCTTACAAG AAGACCAAGC TAACCAAATT GGAAACGCGC 
ACTTCGACCA TCTGCCAGAA GGAGAACAGC TTCTATGTGG ACACGGTGCG AGCTTTTCGC 
GATCGTCGCT ACGAGTACAA AGGACTAACC AAAGTGGCAA AAGCATCGGT GAATGCTGCG 
GTGGCTTCGG GAGACGCGGC AGAGATCAAG GCAGCCAAGG GCAGGGAGGT GCTCTACGAT 
TCCCTGCAGT TGGCCCACAA GTGCATCCTG AACTCCTTCT ATGGCTACGT GATGAGGAGA 
GGAGCCCGTT GGCATTCCAT GCCTATGGCC GGCATTGTGT GCCTCACGGG CTCGAATATT 
ATCACCAAGG CGAGGGAAAT TATCGAGCGA GTTGGTCGAC CACTCGAATT GGACACTGAT 
GGTATATGGT GCATATTGCC TGGCTCCTTT CCGCAGGAGT TTACCATTCA CACGAGCCAT 
GAGAAGAAAA AGAAGATTAA CATATCATAT CCGAATGCAG TGCTAAACAC TATGGTTAAA 
GATCATTTTA CCAACGATCA GTACCACGAG TTGAGGAAGG ATAAGGAAAA CAATCTACCC 
AAATACGATA TTCGAGATGA GAACTCTATA TTCTTCGAGG TGGATGGACC CTACCTTGCC 
ATGGTGTTAC CCGCTGCCAA AGAGGAGGGC AAGAAGCTGA AGAAAAGATA TGCGGTCTTT 
AATTTCGATG GCACACTGGC TGAACTCAAG GGATTCGAGG TGAAGCGACG CGGTGAACTG 
CAGCTGATCA AAAATTTCCA GAGTTCCGTC TTCGAAGCTT TCCTCGCTGG TAGCACACTT 
GAGGAATGCT ATGCATCTGT GGCCAAGGTG GCGGATTACT GGCTTGATGT ACTCTACAGC 
AGAGGATCAA ATCTACCCGA CTCGGAGCTA TTCGAACTTA TTTCGGAGAA CAAGAGCATG 
TCCAAGAAGC TTGAGGAGTA TGGCGCCCAA AAGAGTACGT CCATCTCCAC GGCCAAGCGA 
TTGGCTGAGT TCCTGGGCGA GCAGATGGTA AAGGATGCGG GTCTGGCTTG TAAGTACATT 
ATTTCGAAGA AACCCGAAGG AGCACCCGTC ACCGAGAGAG CTATTCCCTT GGCCATTTTC 
CAATCCGAAC CGAGCGTGAG GCGACATCAC CTGCGTCGCT GGCTTAAGGA CAACACCATG 
GGCGATGCGG ATATACGCGA TGTGCTCGAT TGGAACTACT ACATAGAGAG ATTGGGTGGG 
ACCATTCAGA AGATCATAAC CATACCGGCG GCACTGCAGG GACTGGCCAA TCCAGTGCCC 
AGAGTTCAGC ATCCGGATTG GTTGCACAAG AAAATGCTGG AGAAGAACGA TGTGCTCAAG 
CAGCGTCGCA TCAATGAGAT GTTCACCAGC AGACCCAAAC CGAAACCTCT AGCCACAGAG 
GAGGACAAGC TGGCCGATAT GGAAGATTTG GCTGGTAAAG ATGGCGGTGA GGGTGCTGCA 
GGCTGTCCGA TAGTCACCAA GAGAAAGAGA ATCCAGCTGG AGGAGCACGA TGACGAGGAG 
GCACAGCCGC AGGCCACCAC TTGGCGTCAG GCCTTGGGCG CTCCACCGCC CATCGGTGAA 
ACCAGAAAGA CCATCGTTGA GTGGGTGAGA TTTCAGAAGA AGAAATGGAA ATGGCAGCAG 
GATCAGCGCC AGCGTAATCG CCAGGCGAGC AAGCGAACTC GAGGCGAGGA TCCGCCGGTG 
GTCAGAGCAA CAGGATCCAC AGCTACACTG GGCGGATTCC TTAGACGTGC ACAACGCACC 
CTGTTGGACC AGCCGTGGCA GATCGTACAG TTGGTGCCCG TCGACGACCT GGGCCACTTC 
ACTGTGTGGG CCTTAATCGG CGAAGAGTTG CACAAGATCA AGTTGACGGT ACCGAGGATT 
TTCTATGTAA ATCAGCGAAG TGCAGCTCCT CCAGAGGAGG GTCAACTTTG GCGCAAGGTC 
AATCGAGTTC TGCCACGATC CAGACCTGTT TTCAATCTCT ATCGATATAG TGTGCCCGAA 
CAGCTCTTCC GGGATAACTC GCTGGGCATG CTGGCGGATC TGGCGACGCC CGACATTGAG 
GGCATATACG AGACGCAGAT GACGTTGGAA TTTCGCGCCC TCATGGACAT GGGCTGCATT 
TGCGGTGTCC AGCGCGAGGA GGCACGTCGC TTGGCCCAAT TGGCCACCAA GGATCTGGAA 
ACATTTAGCA TCGAGCAGCT GGAGCAGCGG CCCCAGACTC AGGTCAAATA TTTGGCTAGC 
GCCAACAATC GATTGCGCAA AATCTACTTG TATCAGCATA ACACACCGAC GGCCAAGAAG 
GAGATCTGGT CACTGATCCT AATGCCCAGC AAGAAGGCAT TTGTTTTTGC CTTGGACACA 
GTGCGTGCCA ATCAAATGCC GAACATGAGG CAATTGTATA CCGCCGAGCG TTTGGCCCTG 
CTCAAGAATC TGACGGCAGA GGAGCAAGAT AAAATTCCTG TAGAGGATTA CACATTTGAG 
GTTCTCATTG AGGTGGATGT CAAACAAATT TACCGGCACA TACAGCGGGC ACTGACCACC 
TACAAACAGG AGCATCAGGG ACCCACCATT CTGTGCCTTC AAACGGCGCT GTCGGCGCGT 
AAACTCAGCC TGGCCATGCC GATCCTGCTG GAGTTTCCCC AGGCTGAGAT TCATATCTCC 
GATGACGCTA GTTTGCTTTC TGGCCTTGAT TGGCAGCGAC AGGGCTCCAG GGCAGTGATA 
CGCCACTTTC TGAATCTGAA CAATGTTCTT GATTTGATGT TGGATCAGTG TCGCTACTTC 
CATGTGCCCA TTGGCAATAT GCCGCCGGAT ACTGTGCTTT TCGGAGCGGA TCTTTTCTTC 
GCTCGCTTGC TGCAGCGGCA TAACTTTGTG CTGTGGTGGT CGGCGAGTAC CAGACCAGAT 
TTGGGTGGCC GGGAGGCGGA CGACAGCCGG CTGTTGGCGG AATTCGAGGA GAGCATTAGT 
GTGGTGCAAA ACAAGGCCGG TTTCTATCCG GATGTTTGCG TGGAGCTGGC TCTGGATAGC 
CTGGCGGTGA GTGCCCTGCT CCAATCGACT AGGATTCAGG AAATGGAAGG CGCCTCATCT 
GCCATTACGT TCGATGTGAT GCCGCAGGTC TCGCTGGAGG AGATGATTGG CACTGTTCCG 
GCGGCCACCT TGCCGAGTTA TGATGAAACG GCCCTCTGTT CCGCCGCCTT CCGCGTTATG 
CGCTCCATGG TGAATGGTTG GTTGCGAGAG GTATCCATCA ATAGGAACAT CTTCTCGGAC 
TTCCAGATCG TGCACTTCTA TCGATGGGTG CGCTCCAGTA ATGCACTACT CTATGATCCT 
GCTTTGAGAA GATCTCTGAA TAATCTGATG AGGAAGATGT TCTTGCGCAT TATAGCCGAG 
TTCAAGAGAT TGGGCGCCAC CATTATCTAT GCGGACTTTA ACAGGATTAT CCTTAGTTCG 
GGTAAGAAAA CCGTTTCCGA TGCCCTGGGC TATGTGGACT ACATTGTGCA AAGCTTGAGG 
AACAAGGAGA TGTTCCACTC CATCCAACTG AGCTTCGAGC AATGCTGGAA CTTTATGCTC 
TGGATGGACC AGGCAAATTT CTCGGGAATT AGGGGAAAGC TACCAAAGGG AATCGATGAG 
ACAGTGTCGT CAATAGTTTC CACTACCATG ATACGGGATT CTGAACGCAA TCAAGATGAC 
GACGAGGATG AAGAAGAGGA TTCGGAAAAC CGTGATCCAG TGGAGAGCAA CGAGGCCGAG 
CAGGATCAAG AGGATGAGCT GTCCCTGGAG CTCAACTGGA CAATTGGCGA ACATCTGCCC 
GATGAAAACG AGTGCCGCGA AAAGTTTGAA TCCCTGCTGA CCCTCTTCAT GCAATCTTTG 
GCTGAAAAGA AGACCACCGA GCAGGCCATC AAGGACATCT CACACTGTGC GTTCGACTTT 
ATCCTGAAGC TGCACAAGAA CTACGGCAAG GGCAAGCCGA GCCCGGGCCT AGAACTTATC 
CGCACTCTGA TCAAGGCGTT GAGTGTGGAC AAAACGCTGG CGGAGCAGAT CAACGAGTTG 
CGCCGAAATA TGCTGCGTCT GGTGGGGATT GGTGAGTTCT CCGACTTGGC TGAGTGGGAG 
GATCCCTGCG ACAGTCACAT CATCAACGAG GTCATCTGCA AAGCGTGTAA TCACTGCAGG 
GACCTGGATC TCTGCAAGGA CAAGCATCGC GCCATGAAAG ATGGAGTACC CGTTTGGCTG 
TGTGCCCAGT GCTATGTGGC CTATGATAAC GAGGAGATCG AAATGAGAAT GCTGGATGCA 
CTGCAGCGCA AGATGATGTC CTATGTGCTG CAGGATCTGC GCTGTTCGCG CTGCAGCGAG 
ATCAAGCGCG AGAATCTGGC AGAGTTCTGC ACTTGCGCTG GCAACTTTGT GCCCCTCATC 
AGCGGGAAGG ACATCCAGAC ACTGCTGGGC ACATTCAACA AGGTGGCTGC CAACCACAAG 
ATGCAGTTGC TCCAGCAGAC TGTTCATCAG GCGCTGACCA CGCCACGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524462.2
CDS	64..6774
Translation	MSDSSKGKVLQNTGKFVSENRTEGDDFFNEAGYRQSRENDKIDSKYGFDRVKDSQERTGYLINMHSNEVLDEDRRLIAALDLFFIQMDGSRFKCTVAYQPYLLIRPEDNMHLEVARFLGRKYSGQISGLEHITKEDLDLPNHLSGLQQQYIKLSFLNQTAMTKVRRELMSAVKRNQERQKSNTYYMQMLATSLAQSSAGSEDATLGKRQQDYMDCIVDIREHDVPYHVRVSIDLRIFCGQWYNIRCRSGVELPTITCRPDILDRPEPVVLAFDIETTKLPLKFPDAQTDQVMMISYMIDGQGYLITNREIISSNVDDFEYTPKPEFEGNFIVFNEENEMQLLQRFFDHIMEVRPHIIVTYNGDFFDWPFVETRAAVYDLDMKQEIGFSKLRDGNYLSRPAIHMDCLCWVKRDSYLPVGSQGLKAVAKAKLRYDPVELDPEDMCRMAVEQPQVLANYSVSDAVATYYLYMKYVHPFIFALNTIIPMEPDEILRKGSGTLCETLLMVEAYHAQIVYPNKHQSELNKLSNEGHVLDSETYVGGHVEALESGVFRADIPCRFRLDPAMVKQLQEQVDAVLRHAIEVEEGIPLEKVLNLDEVRQEIVQGLQGLHDIPNRLEQPVIYHLDVGAMYPNIILTNRLQPSAMVSDLDCAACDFNKPGVRCKRSMDWLWRGEMLPASRNEFQRIQQQLETEKFPPLFPGGPQRAFHELSKEDQAAYEKKRLTDYCRKAYKKTKLTKLETRTSTICQKENSFYVDTVRAFRDRRYEYKGLTKVAKASVNAAVASGDAAEIKAAKGREVLYDSLQLAHKCILNSFYGYVMRRGARWHSMPMAGIVCLTGSNIITKAREIIERVGRPLELDTDGIWCILPGSFPQEFTIHTSHEKKKKINISYPNAVLNTMVKDHFTNDQYHELRKDKENNLPKYDIRDENSIFFEVDGPYLAMVLPAAKEEGKKLKKRYAVFNFDGTLAELKGFEVKRRGELQLIKNFQSSVFEAFLAGSTLEECYASVAKVADYWLDVLYSRGSNLPDSELFELISENKSMSKKLEEYGAQKSTSISTAKRLAEFLGEQMVKDAGLACKYIISKKPEGAPVTERAIPLAIFQSEPSVRRHHLRRWLKDNTMGDADIRDVLDWNYYIERLGGTIQKIITIPAALQGLANPVPRVQHPDWLHKKMLEKNDVLKQRRINEMFTSRPKPKPLATEEDKLADMEDLAGKDGGEGAAGCPIVTKRKRIQLEEHDDEEAQPQATTWRQALGAPPPIGETRKTIVEWVRFQKKKWKWQQDQRQRNRQASKRTRGEDPPVVRATGSTATLGGFLRRAQRTLLDQPWQIVQLVPVDDLGHFTVWALIGEELHKIKLTVPRIFYVNQRSAAPPEEGQLWRKVNRVLPRSRPVFNLYRYSVPEQLFRDNSLGMLADLATPDIEGIYETQMTLEFRALMDMGCICGVQREEARRLAQLATKDLETFSIEQLEQRPQTQVKYLASANNRLRKIYLYQHNTPTAKKEIWSLILMPSKKAFVFALDTVRANQMPNMRQLYTAERLALLKNLTAEEQDKIPVEDYTFEVLIEVDVKQIYRHIQRALTTYKQEHQGPTILCLQTALSARKLSLAMPILLEFPQAEIHISDDASLLSGLDWQRQGSRAVIRHFLNLNNVLDLMLDQCRYFHVPIGNMPPDTVLFGADLFFARLLQRHNFVLWWSASTRPDLGGREADDSRLLAEFEESISVVQNKAGFYPDVCVELALDSLAVSALLQSTRIQEMEGASSAITFDVMPQVSLEEMIGTVPAATLPSYDETALCSAAFRVMRSMVNGWLREVSINRNIFSDFQIVHFYRWVRSSNALLYDPALRRSLNNLMRKMFLRIIAEFKRLGATIIYADFNRIILSSGKKTVSDALGYVDYIVQSLRNKEMFHSIQLSFEQCWNFMLWMDQANFSGIRGKLPKGIDETVSSIVSTTMIRDSERNQDDDEDEEEDSENRDPVESNEAEQDQEDELSLELNWTIGEHLPDENECREKFESLLTLFMQSLAEKKTTEQAIKDISHCAFDFILKLHKNYGKGKPSPGLELIRTLIKALSVDKTLAEQINELRRNMLRLVGIGEFSDLAEWEDPCDSHIINEVICKACNHCRDLDLCKDKHRAMKDGVPVWLCAQCYVAYDNEEIEMRMLDALQRKMMSYVLQDLRCSRCSEIKRENLAEFCTCAGNFVPLISGKDIQTLLGTFNKVAANHKMQLLQQTVHQALTTPR

Target ORF information:

RefSeq Version	NM_079738.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster DNA polymerase epsilon 255kD subunit (DNApol-epsilon255), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079738.3

ORF Insert Sequence:

ATGTCCGACT CCAGCAAAGG CAAAGTGCTG CAAAATACGG GTAAATTCGT CAGCGAGAAT 
CGCACAGAAG GCGACGACTT CTTCAATGAG GCGGGCTATC GTCAATCCCG GGAGAACGAT 
AAAATCGACT CGAAATATGG CTTCGATCGG GTTAAGGACA GCCAGGAGCG GACGGGCTAC 
CTCATCAACA TGCATTCGAA CGAAGTTTTG GATGAGGACA GAAGATTGAT TGCTGCCTTG 
GACCTGTTCT TCATCCAAAT GGATGGTTCC CGCTTCAAAT GCACGGTGGC CTATCAGCCA 
TATTTACTCA TCCGACCAGA GGATAATATG CATCTGGAAG TGGCGCGATT TCTGGGTCGC 
AAGTATTCCG GCCAGATTTC TGGACTGGAG CACATAACCA AAGAAGATTT GGATCTGCCC 
AATCATCTAT CCGGTTTGCA GCAGCAGTAC ATAAAACTTT CGTTTCTCAA TCAGACAGCC 
ATGACCAAGG TTAGAAGGGA ACTCATGTCC GCGGTGAAGA GAAATCAGGA GCGACAGAAA 
TCCAACACAT ACTACATGCA AATGCTGGCC ACCTCGCTGG CCCAATCCTC CGCAGGTTCC 
GAGGATGCCA CATTGGGTAA GAGGCAGCAG GATTACATGG ATTGTATTGT GGACATAAGG 
GAGCATGATG TGCCTTACCA CGTCAGAGTG TCCATCGATT TGCGCATCTT TTGTGGACAG 
TGGTACAATA TCAGGTGCAG AAGTGGCGTG GAATTGCCTA CGATCACCTG CCGACCGGAT 
ATTCTGGACA GACCCGAGCC CGTGGTCCTG GCCTTTGATA TAGAAACCAC TAAGCTGCCC 
CTTAAGTTTC CCGATGCCCA GACGGATCAG GTTATGATGA TCTCGTACAT GATCGATGGT 
CAGGGTTATC TGATAACCAA TCGTGAGATT ATATCATCCA ATGTGGACGA TTTTGAGTAC 
ACTCCCAAGC CGGAATTCGA GGGTAACTTT ATAGTATTCA ACGAAGAGAA CGAGATGCAG 
CTGCTCCAGC GCTTCTTCGA TCACATCATG GAGGTGCGTC CCCACATCAT TGTTACATAC 
AACGGCGACT TCTTCGATTG GCCCTTCGTG GAGACGCGTG CTGCAGTGTA CGATCTGGAC 
ATGAAGCAAG AGATTGGCTT CTCCAAGCTA CGGGATGGCA ATTATCTAAG TCGCCCTGCC 
ATACACATGG ATTGCCTATG TTGGGTGAAA CGAGATTCTT ATTTACCTGT TGGATCTCAA 
GGCTTAAAGG CGGTGGCCAA GGCTAAATTA CGCTATGATC CTGTGGAACT CGATCCGGAG 
GATATGTGCC GCATGGCCGT GGAACAGCCC CAAGTGCTGG CCAATTACTC TGTATCCGAT 
GCGGTGGCCA CATACTATCT GTACATGAAG TATGTGCATC CATTTATCTT CGCCCTAAAT 
ACGATTATTC CCATGGAACC CGATGAGATC CTAAGAAAGG GTTCCGGCAC ACTCTGTGAA 
ACGTTGCTGA TGGTGGAGGC TTACCATGCC CAGATTGTGT ATCCCAACAA GCATCAGAGT 
GAGCTGAATA AGCTCTCCAA CGAGGGACAC GTACTGGATT CGGAAACCTA TGTGGGTGGT 
CATGTGGAGG CTTTGGAATC GGGTGTTTTC CGGGCGGACA TACCATGCCG TTTTCGTCTA 
GATCCTGCTA TGGTCAAGCA ACTGCAGGAG CAGGTTGATG CAGTTCTGCG CCACGCTATC 
GAAGTGGAGG AAGGCATACC GCTCGAGAAG GTCTTGAATC TGGATGAAGT GCGGCAGGAG 
ATTGTGCAGG GGCTACAGGG TCTGCACGAT ATACCCAATC GCTTGGAGCA GCCGGTCATC 
TATCACTTGG ATGTGGGTGC CATGTACCCC AACATTATTT TGACCAATCG CCTGCAGCCC 
TCGGCAATGG TTAGTGACTT AGATTGTGCC GCCTGTGACT TCAACAAGCC AGGAGTTCGG 
TGCAAACGTT CCATGGACTG GTTGTGGCGC GGCGAGATGT TGCCCGCCTC CAGGAACGAG 
TTTCAGCGCA TTCAGCAGCA GCTGGAGACC GAGAAGTTTC CACCCCTTTT CCCTGGCGGA 
CCACAGCGAG CCTTTCACGA GCTCTCCAAG GAGGATCAGG CGGCGTACGA GAAGAAACGT 
CTGACGGATT ACTGCCGCAA GGCTTACAAG AAGACCAAGC TAACCAAATT GGAAACGCGC 
ACTTCGACCA TCTGCCAGAA GGAGAACAGC TTCTATGTGG ACACGGTGCG AGCTTTTCGC 
GATCGTCGCT ACGAGTACAA AGGACTAACC AAAGTGGCAA AAGCATCGGT GAATGCTGCG 
GTGGCTTCGG GAGACGCGGC AGAGATCAAG GCAGCCAAGG GCAGGGAGGT GCTCTACGAT 
TCCCTGCAGT TGGCCCACAA GTGCATCCTG AACTCCTTCT ATGGCTACGT GATGAGGAGA 
GGAGCCCGTT GGCATTCCAT GCCTATGGCC GGCATTGTGT GCCTCACGGG CTCGAATATT 
ATCACCAAGG CGAGGGAAAT TATCGAGCGA GTTGGTCGAC CACTCGAATT GGACACTGAT 
GGTATATGGT GCATATTGCC TGGCTCCTTT CCGCAGGAGT TTACCATTCA CACGAGCCAT 
GAGAAGAAAA AGAAGATTAA CATATCATAT CCGAATGCAG TGCTAAACAC TATGGTTAAA 
GATCATTTTA CCAACGATCA GTACCACGAG TTGAGGAAGG ATAAGGAAAA CAATCTACCC 
AAATACGATA TTCGAGATGA GAACTCTATA TTCTTCGAGG TGGATGGACC CTACCTTGCC 
ATGGTGTTAC CCGCTGCCAA AGAGGAGGGC AAGAAGCTGA AGAAAAGATA TGCGGTCTTT 
AATTTCGATG GCACACTGGC TGAACTCAAG GGATTCGAGG TGAAGCGACG CGGTGAACTG 
CAGCTGATCA AAAATTTCCA GAGTTCCGTC TTCGAAGCTT TCCTCGCTGG TAGCACACTT 
GAGGAATGCT ATGCATCTGT GGCCAAGGTG GCGGATTACT GGCTTGATGT ACTCTACAGC 
AGAGGATCAA ATCTACCCGA CTCGGAGCTA TTCGAACTTA TTTCGGAGAA CAAGAGCATG 
TCCAAGAAGC TTGAGGAGTA TGGCGCCCAA AAGAGTACGT CCATCTCCAC GGCCAAGCGA 
TTGGCTGAGT TCCTGGGCGA GCAGATGGTA AAGGATGCGG GTCTGGCTTG TAAGTACATT 
ATTTCGAAGA AACCCGAAGG AGCACCCGTC ACCGAGAGAG CTATTCCCTT GGCCATTTTC 
CAATCCGAAC CGAGCGTGAG GCGACATCAC CTGCGTCGCT GGCTTAAGGA CAACACCATG 
GGCGATGCGG ATATACGCGA TGTGCTCGAT TGGAACTACT ACATAGAGAG ATTGGGTGGG 
ACCATTCAGA AGATCATAAC CATACCGGCG GCACTGCAGG GACTGGCCAA TCCAGTGCCC 
AGAGTTCAGC ATCCGGATTG GTTGCACAAG AAAATGCTGG AGAAGAACGA TGTGCTCAAG 
CAGCGTCGCA TCAATGAGAT GTTCACCAGC AGACCCAAAC CGAAACCTCT AGCCACAGAG 
GAGGACAAGC TGGCCGATAT GGAAGATTTG GCTGGTAAAG ATGGCGGTGA GGGTGCTGCA 
GGCTGTCCGA TAGTCACCAA GAGAAAGAGA ATCCAGCTGG AGGAGCACGA TGACGAGGAG 
GCACAGCCGC AGGCCACCAC TTGGCGTCAG GCCTTGGGCG CTCCACCGCC CATCGGTGAA 
ACCAGAAAGA CCATCGTTGA GTGGGTGAGA TTTCAGAAGA AGAAATGGAA ATGGCAGCAG 
GATCAGCGCC AGCGTAATCG CCAGGCGAGC AAGCGAACTC GAGGCGAGGA TCCGCCGGTG 
GTCAGAGCAA CAGGATCCAC AGCTACACTG GGCGGATTCC TTAGACGTGC ACAACGCACC 
CTGTTGGACC AGCCGTGGCA GATCGTACAG TTGGTGCCCG TCGACGACCT GGGCCACTTC 
ACTGTGTGGG CCTTAATCGG CGAAGAGTTG CACAAGATCA AGTTGACGGT ACCGAGGATT 
TTCTATGTAA ATCAGCGAAG TGCAGCTCCT CCAGAGGAGG GTCAACTTTG GCGCAAGGTC 
AATCGAGTTC TGCCACGATC CAGACCTGTT TTCAATCTCT ATCGATATAG TGTGCCCGAA 
CAGCTCTTCC GGGATAACTC GCTGGGCATG CTGGCGGATC TGGCGACGCC CGACATTGAG 
GGCATATACG AGACGCAGAT GACGTTGGAA TTTCGCGCCC TCATGGACAT GGGCTGCATT 
TGCGGTGTCC AGCGCGAGGA GGCACGTCGC TTGGCCCAAT TGGCCACCAA GGATCTGGAA 
ACATTTAGCA TCGAGCAGCT GGAGCAGCGG CCCCAGACTC AGGTCAAATA TTTGGCTAGC 
GCCAACAATC GATTGCGCAA AATCTACTTG TATCAGCATA ACACACCGAC GGCCAAGAAG 
GAGATCTGGT CACTGATCCT AATGCCCAGC AAGAAGGCAT TTGTTTTTGC CTTGGACACA 
GTGCGTGCCA ATCAAATGCC GAACATGAGG CAATTGTATA CCGCCGAGCG TTTGGCCCTG 
CTCAAGAATC TGACGGCAGA GGAGCAAGAT AAAATTCCTG TAGAGGATTA CACATTTGAG 
GTTCTCATTG AGGTGGATGT CAAACAAATT TACCGGCACA TACAGCGGGC ACTGACCACC 
TACAAACAGG AGCATCAGGG ACCCACCATT CTGTGCCTTC AAACGGCGCT GTCGGCGCGT 
AAACTCAGCC TGGCCATGCC GATCCTGCTG GAGTTTCCCC AGGCTGAGAT TCATATCTCC 
GATGACGCTA GTTTGCTTTC TGGCCTTGAT TGGCAGCGAC AGGGCTCCAG GGCAGTGATA 
CGCCACTTTC TGAATCTGAA CAATGTTCTT GATTTGATGT TGGATCAGTG TCGCTACTTC 
CATGTGCCCA TTGGCAATAT GCCGCCGGAT ACTGTGCTTT TCGGAGCGGA TCTTTTCTTC 
GCTCGCTTGC TGCAGCGGCA TAACTTTGTG CTGTGGTGGT CGGCGAGTAC CAGACCAGAT 
TTGGGTGGCC GGGAGGCGGA CGACAGCCGG CTGTTGGCGG AATTCGAGGA GAGCATTAGT 
GTGGTGCAAA ACAAGGCCGG TTTCTATCCG GATGTTTGCG TGGAGCTGGC TCTGGATAGC 
CTGGCGGTGA GTGCCCTGCT CCAATCGACT AGGATTCAGG AAATGGAAGG CGCCTCATCT 
GCCATTACGT TCGATGTGAT GCCGCAGGTC TCGCTGGAGG AGATGATTGG CACTGTTCCG 
GCGGCCACCT TGCCGAGTTA TGATGAAACG GCCCTCTGTT CCGCCGCCTT CCGCGTTATG 
CGCTCCATGG TGAATGGTTG GTTGCGAGAG GTATCCATCA ATAGGAACAT CTTCTCGGAC 
TTCCAGATCG TGCACTTCTA TCGATGGGTG CGCTCCAGTA ATGCACTACT CTATGATCCT 
GCTTTGAGAA GATCTCTGAA TAATCTGATG AGGAAGATGT TCTTGCGCAT TATAGCCGAG 
TTCAAGAGAT TGGGCGCCAC CATTATCTAT GCGGACTTTA ACAGGATTAT CCTTAGTTCG 
GGTAAGAAAA CCGTTTCCGA TGCCCTGGGC TATGTGGACT ACATTGTGCA AAGCTTGAGG 
AACAAGGAGA TGTTCCACTC CATCCAACTG AGCTTCGAGC AATGCTGGAA CTTTATGCTC 
TGGATGGACC AGGCAAATTT CTCGGGAATT AGGGGAAAGC TACCAAAGGG AATCGATGAG 
ACAGTGTCGT CAATAGTTTC CACTACCATG ATACGGGATT CTGAACGCAA TCAAGATGAC 
GACGAGGATG AAGAAGAGGA TTCGGAAAAC CGTGATCCAG TGGAGAGCAA CGAGGCCGAG 
CAGGATCAAG AGGATGAGCT GTCCCTGGAG CTCAACTGGA CAATTGGCGA ACATCTGCCC 
GATGAAAACG AGTGCCGCGA AAAGTTTGAA TCCCTGCTGA CCCTCTTCAT GCAATCTTTG 
GCTGAAAAGA AGACCACCGA GCAGGCCATC AAGGACATCT CACACTGTGC GTTCGACTTT 
ATCCTGAAGC TGCACAAGAA CTACGGCAAG GGCAAGCCGA GCCCGGGCCT AGAACTTATC 
CGCACTCTGA TCAAGGCGTT GAGTGTGGAC AAAACGCTGG CGGAGCAGAT CAACGAGTTG 
CGCCGAAATA TGCTGCGTCT GGTGGGGATT GGTGAGTTCT CCGACTTGGC TGAGTGGGAG 
GATCCCTGCG ACAGTCACAT CATCAACGAG GTCATCTGCA AAGCGTGTAA TCACTGCAGG 
GACCTGGATC TCTGCAAGGA CAAGCATCGC GCCATGAAAG ATGGAGTACC CGTTTGGCTG 
TGTGCCCAGT GCTATGTGGC CTATGATAAC GAGGAGATCG AAATGAGAAT GCTGGATGCA 
CTGCAGCGCA AGATGATGTC CTATGTGCTG CAGGATCTGC GCTGTTCGCG CTGCAGCGAG 
ATCAAGCGCG AGAATCTGGC AGAGTTCTGC ACTTGCGCTG GCAACTTTGT GCCCCTCATC 
AGCGGGAAGG ACATCCAGAC ACTGCTGGGC ACATTCAACA AGGTGGCTGC CAACCACAAG 
ATGCAGTTGC TCCAGCAGAC TGTTCATCAG GCGCTGACCA CGCCACGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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DNApol-epsilon255 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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