bb8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bb8
Entrez Gene ID	42756
Full Name	CG4434 gene product from transcript CG4434-RA
Synonyms	Dmel\CG4434
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94E9-94E9

mRNA and Protein(s)

mRNA	Protein	Name
NM_142883.2	NP_651140.1	big bubble 8

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	GDH3	NP_187041.1
Drosophila melanogaster (fruit fly)	CG4434	NP_651140.1
Arabidopsis thaliana (thale cress)	GDH1	NP_197318.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004622	XP_315196.5
Oryza sativa (rice)	Os03g0794500	NP_001051540.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following bb8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bb8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05778	NM_142883.2 Latest version!	Drosophila melanogaster big bubble 8 (bb8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bb8 ( NM_142883.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651140.1 Drosophila melanogaster big bubble 8 (bb8), mRNA.

Price & Availability↑

CloneID	OFa05778
Clone ID Related Accession (Same CDS sequence)	NM_142883.2
Accession Version	NM_142883.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	big bubble 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_142883.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTCGTT ATACGGCACG GATTTTGAAT GGGATTTACC CACTTATGCC AAGGCAAGTA 
TTAAAACGTT CCGCACACCA GGTGCCCGAG AAGCTGAAAA AAGTTGAGAC GGACAAGGAC 
CCGGAATTCT CGGAAATGGT TCTTTATTAT TATCACAAGG CCGCGCAGAC TATGGAGCCT 
GCATTGCTCA AGGAAATGGA AAAGTATCCG CATATGAAGC CGGAGGAGCG TCAGGCTCGT 
GTCACCGCTA TACTGAATCT GCTTGGGAGC GTCTCCACCT CGGTGGAGGT TAATTTTCCC 
ATTGTCCGCA AAAACGGCAC CTACGAGATC ATCAGCGGTT ATCGTTCGCA TCATGTACGC 
CATCGATTGC CTCTCAAGGG CGGAATCCGA TACGCTCTGG ACGTGAACGA GAGCGAAGTT 
AAAGCCCTTG CCGCCATTAT GACGTTCAAG TGCGCTTGCG TGAATGTACC TTATGGTGGA 
TCTAAGGGCG GCATTTGTAT CGATCCCAAG AAGTATACAG TGGATGAGCT GCAGACGATC 
ACACGGCGAT ACACGATGGA GTTGCTCAAG CGTAATATGA TTGGTCCCGG CATCGATGTA 
CCGGCGCCCG ATGTGAACAC TGGTCCGCGT GAGATGAGCT GGATTGTTGA TCAGTACCAG 
AAGACATTTG GCTACAAGGA TATCAATTCA TCGGCTATTG TCACCGGGAA GCCCGTACAC 
AACGGCGGCA TTAATGGTCG ACATTCGGCC ACGGGCAGAG GCGTTTGGAA GGCGGGCGAT 
CTCTTTCTGA AGGATAAGGA ATGGATGGAT CTGCTCAAGT GGAAGACGGG ATGGAAGGAC 
AAGACGGTCA TAGTGCAGGG ATTTGGCAAT GTGGGCTCCT TTGCCGCTAA GTATGTGCAC 
GAGGCGGGCG CCAAAGTCAT CGGAATAAAA GAGTTTGATG TTTCTCTGGT TAATAAGGAC 
GGCATAGATA TTAATGATCT TTTTGAATAC ACTGAGGAAA AGAAGACTAT TAAAGGATAT 
CCAAAAGCTC AGGAGTCTAA GGAGGACTTG TTGGTAGCCG AAACCGATAT CCTTATGCCG 
TGTGCCACCC AAAAAGTGAT AACCACCGAC AATGCCAAAG ACATCAAGGC CAAGCTGATC 
CTGGAAGGCG CCAACGGGCC GACCACACCA TCTGGCGAGA AAATCCTATT GGATAAGGGT 
GTCCTCCTAG TTCCCGATCT CTATTGCAAT GCGGGCGGCG TAACAGTATC CTACTTTGAG 
TACCTCAAAA ACATTAACCA CGTGTCATAT GGCAAAATGA ACTCTAAATC TACATCCGAG 
CTTATTATCG AACTGATGAA CTCCATCAAT GAGTCTTTGC ATGAATGCCC CGATAGTCAA 
CTACCGAACA TATGTCCCAA TAAGAAACTT AAGAGGATAC AACAATGCAC CACAGAGGCG 
GATATCGTGG ACTCGGCTCT CCAAACAGTT ATGGAATCTG CAGCACGTGG CATCAAGGAG 
ATGGCCCACA AATTTGAATT GTGTAACGAT CTGCGGAGGG CCGCCTACGT TTGGTCTTCA 
TTCAAAATTT TCCAAGCCAT GGAAAGCTCC GGAATTTCCC AACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651140.1
CDS	92..1699
Translation	MLRYTARILNGIYPLMPRQVLKRSAHQVPEKLKKVETDKDPEFSEMVLYYYHKAAQTMEPALLKEMEKYPHMKPEERQARVTAILNLLGSVSTSVEVNFPIVRKNGTYEIISGYRSHHVRHRLPLKGGIRYALDVNESEVKALAAIMTFKCACVNVPYGGSKGGICIDPKKYTVDELQTITRRYTMELLKRNMIGPGIDVPAPDVNTGPREMSWIVDQYQKTFGYKDINSSAIVTGKPVHNGGINGRHSATGRGVWKAGDLFLKDKEWMDLLKWKTGWKDKTVIVQGFGNVGSFAAKYVHEAGAKVIGIKEFDVSLVNKDGIDINDLFEYTEEKKTIKGYPKAQESKEDLLVAETDILMPCATQKVITTDNAKDIKAKLILEGANGPTTPSGEKILLDKGVLLVPDLYCNAGGVTVSYFEYLKNINHVSYGKMNSKSTSELIIELMNSINESLHECPDSQLPNICPNKKLKRIQQCTTEADIVDSALQTVMESAARGIKEMAHKFELCNDLRRAAYVWSSFKIFQAMESSGISQQ

Target ORF information:

RefSeq Version	NM_142883.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster big bubble 8 (bb8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142883.2

ORF Insert Sequence:

ATGCTTCGTT ATACGGCACG GATTTTGAAT GGGATTTACC CACTTATGCC AAGGCAAGTA 
TTAAAACGTT CCGCACACCA GGTGCCCGAG AAGCTGAAAA AAGTTGAGAC GGACAAGGAC 
CCGGAATTCT CGGAAATGGT TCTTTATTAT TATCACAAGG CCGCGCAGAC TATGGAGCCT 
GCATTGCTCA AGGAAATGGA AAAGTATCCG CATATGAAGC CGGAGGAGCG TCAGGCTCGT 
GTCACCGCTA TACTGAATCT GCTTGGGAGC GTCTCCACCT CGGTGGAGGT TAATTTTCCC 
ATTGTCCGCA AAAACGGCAC CTACGAGATC ATCAGCGGTT ATCGTTCGCA TCATGTACGC 
CATCGATTGC CTCTCAAGGG CGGAATCCGA TACGCTCTGG ACGTGAACGA GAGCGAAGTT 
AAAGCCCTTG CCGCCATTAT GACGTTCAAG TGCGCTTGCG TGAATGTACC TTATGGTGGA 
TCTAAGGGCG GCATTTGTAT CGATCCCAAG AAGTATACAG TGGATGAGCT GCAGACGATC 
ACACGGCGAT ACACGATGGA GTTGCTCAAG CGTAATATGA TTGGTCCCGG CATCGATGTA 
CCGGCGCCCG ATGTGAACAC TGGTCCGCGT GAGATGAGCT GGATTGTTGA TCAGTACCAG 
AAGACATTTG GCTACAAGGA TATCAATTCA TCGGCTATTG TCACCGGGAA GCCCGTACAC 
AACGGCGGCA TTAATGGTCG ACATTCGGCC ACGGGCAGAG GCGTTTGGAA GGCGGGCGAT 
CTCTTTCTGA AGGATAAGGA ATGGATGGAT CTGCTCAAGT GGAAGACGGG ATGGAAGGAC 
AAGACGGTCA TAGTGCAGGG ATTTGGCAAT GTGGGCTCCT TTGCCGCTAA GTATGTGCAC 
GAGGCGGGCG CCAAAGTCAT CGGAATAAAA GAGTTTGATG TTTCTCTGGT TAATAAGGAC 
GGCATAGATA TTAATGATCT TTTTGAATAC ACTGAGGAAA AGAAGACTAT TAAAGGATAT 
CCAAAAGCTC AGGAGTCTAA GGAGGACTTG TTGGTAGCCG AAACCGATAT CCTTATGCCG 
TGTGCCACCC AAAAAGTGAT AACCACCGAC AATGCCAAAG ACATCAAGGC CAAGCTGATC 
CTGGAAGGCG CCAACGGGCC GACCACACCA TCTGGCGAGA AAATCCTATT GGATAAGGGT 
GTCCTCCTAG TTCCCGATCT CTATTGCAAT GCGGGCGGCG TAACAGTATC CTACTTTGAG 
TACCTCAAAA ACATTAACCA CGTGTCATAT GGCAAAATGA ACTCTAAATC TACATCCGAG 
CTTATTATCG AACTGATGAA CTCCATCAAT GAGTCTTTGC ATGAATGCCC CGATAGTCAA 
CTACCGAACA TATGTCCCAA TAAGAAACTT AAGAGGATAC AACAATGCAC CACAGAGGCG 
GATATCGTGG ACTCGGCTCT CCAAACAGTT ATGGAATCTG CAGCACGTGG CATCAAGGAG 
ATGGCCCACA AATTTGAATT GTGTAACGAT CTGCGGAGGG CCGCCTACGT TTGGTCTTCA 
TTCAAAATTT TCCAAGCCAT GGAAAGCTCC GGAATTTCCC AACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bb8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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