wda cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wda
Entrez Gene ID	42750
Full Name	will decrease acetylation
Synonyms	CG4448,Dmel\CG4448,WDA,WFA,Wda
General protein information	Preferred Names will decrease acetylation Names CG4448 gene product from transcript CG4448-RA CG4448-PA wda-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94E9-94E9

mRNA and Protein(s)

mRNA	Protein	Name
NM_142879.3	NP_651136.1	will decrease acetylation

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003524	XP_313266.5
Drosophila melanogaster (fruit fly)	wda	NP_651136.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results point to a critical function of dSAGA and histone acetylation during Drosophila development.
Title: The essential gene wda encodes a WD40 repeat subunit of Drosophila SAGA required for histone H3 acetylation.

The following wda gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wda cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05770	NM_142879.3 Latest version!	Drosophila melanogaster will decrease acetylation (wda), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wda ( NM_142879.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651136.1 Drosophila melanogaster will decrease acetylation (wda), mRNA.

Price & Availability↑

CloneID	OFa05770
Clone ID Related Accession (Same CDS sequence)	NM_142879.3
Accession Version	NM_142879.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	will decrease acetylation
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142879.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCTGC CCAGCGCCAG CAACCACGCA CATGCTGCAT CGCCCGTCTA CAGCAACAGC 
AATAACAACA AGTCGAGCGC GAGCAGCAAA AAGACCTCCT CCAAAAACGA TTTGCTACGC 
TGCGCCGTGG GACTCCTGCT GAAGCAGAAA AACTACGTGA GCAACGAACG ATTTCGGCGG 
TCGGATTTTT TGCTGCTGCA AAACAAACAG CAGTTTGCTG TCAACAAAAT GCTGGATACG 
GATTTGCACG GTGGCAATAG CTTCACCTTT TCAAATGTCC AGGTTATCAC CAACAACCAG 
CACACTGTGG ACCAGCAATT TGGTCGGTTT TCGCAGTTCG TGGAGGCCCA GGCCGAACCG 
CTGCGTCTGG AAATGAAGCG CTTTTATGGA CCCATGCTGT GCCACTTCTA TCTGGATCTG 
CTCAAGGCTC GCGAACCGCG TGGCGCTGTG GAGTTGTTGC GCAAATATGC TCACTTGGTG 
GCCCCCGTGG ATATGTACGA TGCACCACCA CCTACCAAGA TCAACGGATG CTCCACTACG 
GCCAATGAGT CCACTTTTAA CATACGCTTC GCAAAGGAAG CCCAGGACAC TGGGGACACG 
GAACTGGACT ACTTTATGCG CCTGGTGCAA ACCCTGAGTG GCTACACACG GCTGGAGGCA 
GCAGAGTCCG ATGACACAGT GGCCCATTTT CGATCTTCGA AATACGAACT ACATACTACA 
GCCGTAGTTG TTAATAGGAT ATGCGCTTAT CTCCAACGAC GGGGCCACGT TTTAATTATG 
AACCTACTCT ACACCTGGTT GCATGTCCAT ATAGTGGAGA ATGAGCAGCG CGCCTTTAGT 
GAGGATCACC TGCTTGGCTT GACGGATGAC CTGGAAGGTG AGGATGGAGA GGACGATGTT 
GTTTCCAAAC CAGCAGTAAC CCTTAATACC CGCGGAGACA TAAGACCAAG CAAATCCCTC 
ACTGAAAAGT CGAACCGAAA ACGACCCGCT GAGGAGCCAA ACATTATGCT AGAAACAGAC 
ATCAAACAGG AGGTAGAAAC GGATGAATCT GCAGAGCTTA GTAAATTACA ACTGAATATA 
GACGCCTGCT TGGACACCCT TAAATCGGCC ACTGAACAGA TACTCAAGTC TCAGGTGGAG 
TTGCCGCGAT TTCTCAGAAT CAGTGAACGT TCTCGGGGCC TCACCTCCGC CCATCTGGAT 
CCCAGTGAGT GCCACATGTT AGCCGGGTTC GACAATTCTG CCGTACAGCT GTGGCAGTTA 
AACCAGAGCT ACTGCCGCGG CAAGAGCTTC TACAGACGCT ATCCGCAAAA GCGGTGTCCC 
TGGGAGCTGA ACAATTGCGC GAATCAAGAG GAGGAGACGG ATGAGGACAG CAGCGATGAG 
GATGTCAAGT GCTCTGAGGA GGAGAGACGG GAACGCAACA GGGCGCGACA TTGCAAATAT 
GCTGATAACT CCTACAACGA ATACGGCGGA TTTCAGCTGC GAGGTCACAC AAAAGGAGTC 
ACCGATGTGC GCTTCTCCGC CCACTACCCA CTCATGTATA GTGTGTCCAA AGACGCAACC 
ATGCGGTGCT GGCGTGCCCA CAACCTGCAT TGCGCCGCCA TATACAGAAG TCACAACTAC 
CCAATTTGGT GCTTGGACGA AAGTCCAGTG GGCCAATACG TGGTAACGGG TTCCAAGGAT 
TTGAGTGCGC GCCTCTGGTC GCTAGAGAAG GAACATGCGC TCATAATTTA TGCCGGGCAT 
ACACAGGATG TTGAGTGCGT TGCGTTCCAT CCGAATGGCA ACTACATAGC CACAGGATCG 
GCAGATCACT CCGTTCGCCT GTGGTGTGCA ACCAGCGGAA AATTGATGCG TGTCTTTGCC 
GACTGCCGTC AGGCGGTGAC CCAGCTGGCG TTTAGCCCAG ATGGAAAGAT GTTGGCTGCC 
GCCGGAGAGG AAACGAAGGT GCGTATATTC GACTTGGCCG CCGGAGCACA GCTGGCTGAG 
CTCAAGGATC ACTCGGCGAG CATTAGTTCT CTTTCCTGGA GCACGCACAA TAGGCACCTG 
GCCACAGCGT GCAGCGATGG TACCTTGCGA TTGTGGGACA TCAAGAAACT ATCGCCCATG 
AGTGACAACA GTAGTGCTGG AAGCTCATCA TCAGCTACAA CGAATCGTGT GCTTACTGTC 
AATAGTTCCT GCCAGCGCTT GGTCGATGTT TTTTACGGGA CCAGCAAGAC GCTCTACTGC 
ATCGGCACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651136.1
CDS	89..2320
Translation	MSLPSASNHAHAASPVYSNSNNNKSSASSKKTSSKNDLLRCAVGLLLKQKNYVSNERFRRSDFLLLQNKQQFAVNKMLDTDLHGGNSFTFSNVQVITNNQHTVDQQFGRFSQFVEAQAEPLRLEMKRFYGPMLCHFYLDLLKAREPRGAVELLRKYAHLVAPVDMYDAPPPTKINGCSTTANESTFNIRFAKEAQDTGDTELDYFMRLVQTLSGYTRLEAAESDDTVAHFRSSKYELHTTAVVVNRICAYLQRRGHVLIMNLLYTWLHVHIVENEQRAFSEDHLLGLTDDLEGEDGEDDVVSKPAVTLNTRGDIRPSKSLTEKSNRKRPAEEPNIMLETDIKQEVETDESAELSKLQLNIDACLDTLKSATEQILKSQVELPRFLRISERSRGLTSAHLDPSECHMLAGFDNSAVQLWQLNQSYCRGKSFYRRYPQKRCPWELNNCANQEEETDEDSSDEDVKCSEEERRERNRARHCKYADNSYNEYGGFQLRGHTKGVTDVRFSAHYPLMYSVSKDATMRCWRAHNLHCAAIYRSHNYPIWCLDESPVGQYVVTGSKDLSARLWSLEKEHALIIYAGHTQDVECVAFHPNGNYIATGSADHSVRLWCATSGKLMRVFADCRQAVTQLAFSPDGKMLAAAGEETKVRIFDLAAGAQLAELKDHSASISSLSWSTHNRHLATACSDGTLRLWDIKKLSPMSDNSSAGSSSSATTNRVLTVNSSCQRLVDVFYGTSKTLYCIGT

Target ORF information:

RefSeq Version	NM_142879.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster will decrease acetylation (wda), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142879.3

ORF Insert Sequence:

ATGAGCCTGC CCAGCGCCAG CAACCACGCA CATGCTGCAT CGCCCGTCTA CAGCAACAGC 
AATAACAACA AGTCGAGCGC GAGCAGCAAA AAGACCTCCT CCAAAAACGA TTTGCTACGC 
TGCGCCGTGG GACTCCTGCT GAAGCAGAAA AACTACGTGA GCAACGAACG ATTTCGGCGG 
TCGGATTTTT TGCTGCTGCA AAACAAACAG CAGTTTGCTG TCAACAAAAT GCTGGATACG 
GATTTGCACG GTGGCAATAG CTTCACCTTT TCAAATGTCC AGGTTATCAC CAACAACCAG 
CACACTGTGG ACCAGCAATT TGGTCGGTTT TCGCAGTTCG TGGAGGCCCA GGCCGAACCG 
CTGCGTCTGG AAATGAAGCG CTTTTATGGA CCCATGCTGT GCCACTTCTA TCTGGATCTG 
CTCAAGGCTC GCGAACCGCG TGGCGCTGTG GAGTTGTTGC GCAAATATGC TCACTTGGTG 
GCCCCCGTGG ATATGTACGA TGCACCACCA CCTACCAAGA TCAACGGATG CTCCACTACG 
GCCAATGAGT CCACTTTTAA CATACGCTTC GCAAAGGAAG CCCAGGACAC TGGGGACACG 
GAACTGGACT ACTTTATGCG CCTGGTGCAA ACCCTGAGTG GCTACACACG GCTGGAGGCA 
GCAGAGTCCG ATGACACAGT GGCCCATTTT CGATCTTCGA AATACGAACT ACATACTACA 
GCCGTAGTTG TTAATAGGAT ATGCGCTTAT CTCCAACGAC GGGGCCACGT TTTAATTATG 
AACCTACTCT ACACCTGGTT GCATGTCCAT ATAGTGGAGA ATGAGCAGCG CGCCTTTAGT 
GAGGATCACC TGCTTGGCTT GACGGATGAC CTGGAAGGTG AGGATGGAGA GGACGATGTT 
GTTTCCAAAC CAGCAGTAAC CCTTAATACC CGCGGAGACA TAAGACCAAG CAAATCCCTC 
ACTGAAAAGT CGAACCGAAA ACGACCCGCT GAGGAGCCAA ACATTATGCT AGAAACAGAC 
ATCAAACAGG AGGTAGAAAC GGATGAATCT GCAGAGCTTA GTAAATTACA ACTGAATATA 
GACGCCTGCT TGGACACCCT TAAATCGGCC ACTGAACAGA TACTCAAGTC TCAGGTGGAG 
TTGCCGCGAT TTCTCAGAAT CAGTGAACGT TCTCGGGGCC TCACCTCCGC CCATCTGGAT 
CCCAGTGAGT GCCACATGTT AGCCGGGTTC GACAATTCTG CCGTACAGCT GTGGCAGTTA 
AACCAGAGCT ACTGCCGCGG CAAGAGCTTC TACAGACGCT ATCCGCAAAA GCGGTGTCCC 
TGGGAGCTGA ACAATTGCGC GAATCAAGAG GAGGAGACGG ATGAGGACAG CAGCGATGAG 
GATGTCAAGT GCTCTGAGGA GGAGAGACGG GAACGCAACA GGGCGCGACA TTGCAAATAT 
GCTGATAACT CCTACAACGA ATACGGCGGA TTTCAGCTGC GAGGTCACAC AAAAGGAGTC 
ACCGATGTGC GCTTCTCCGC CCACTACCCA CTCATGTATA GTGTGTCCAA AGACGCAACC 
ATGCGGTGCT GGCGTGCCCA CAACCTGCAT TGCGCCGCCA TATACAGAAG TCACAACTAC 
CCAATTTGGT GCTTGGACGA AAGTCCAGTG GGCCAATACG TGGTAACGGG TTCCAAGGAT 
TTGAGTGCGC GCCTCTGGTC GCTAGAGAAG GAACATGCGC TCATAATTTA TGCCGGGCAT 
ACACAGGATG TTGAGTGCGT TGCGTTCCAT CCGAATGGCA ACTACATAGC CACAGGATCG 
GCAGATCACT CCGTTCGCCT GTGGTGTGCA ACCAGCGGAA AATTGATGCG TGTCTTTGCC 
GACTGCCGTC AGGCGGTGAC CCAGCTGGCG TTTAGCCCAG ATGGAAAGAT GTTGGCTGCC 
GCCGGAGAGG AAACGAAGGT GCGTATATTC GACTTGGCCG CCGGAGCACA GCTGGCTGAG 
CTCAAGGATC ACTCGGCGAG CATTAGTTCT CTTTCCTGGA GCACGCACAA TAGGCACCTG 
GCCACAGCGT GCAGCGATGG TACCTTGCGA TTGTGGGACA TCAAGAAACT ATCGCCCATG 
AGTGACAACA GTAGTGCTGG AAGCTCATCA TCAGCTACAA CGAATCGTGT GCTTACTGTC 
AATAGTTCCT GCCAGCGCTT GGTCGATGTT TTTTACGGGA CCAGCAAGAC GCTCTACTGC 
ATCGGCACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wda cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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