pit cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pit
Entrez Gene ID	42595
Full Name	pitchoune
Synonyms	CG6375,DmRH15,Dmel\CG6375,Hel93F,PIT,Pit,hel93F
General protein information	Preferred Names pitchoune Names CG6375 gene product from transcript CG6375-RB CG6375-PA CG6375-PB CG6375-PC helicase at 93F pit-PA pit-PB pit-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94A1-94A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079722.4	NP_524446.3	pitchoune, isoform A
NM_001300530.1	NP_001287459.1	pitchoune, isoform C
NM_169991.2	NP_732694.2	pitchoune, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDX18	NP_006764.3
Bos taurus (cattle)	DDX18	XP_002685472.1
Danio rerio (zebrafish)	ddx18	NP_001003411.1
Drosophila melanogaster (fruit fly)	pit	NP_732694.2
Eremothecium gossypii	AGOS_AGR333C	NP_986999.2
Arabidopsis thaliana (thale cress)	AT3G18600	NP_188490.1
Pan troglodytes (chimpanzee)	DDX18	XP_515753.2
Canis lupus familiaris (dog)	DDX18	XP_533327.3
Mus musculus (house mouse)	Ddx18	NP_080136.2
Caenorhabditis elegans (roundworm)	B0511.6	NP_492779.1
Saccharomyces cerevisiae (baker's yeast)	HAS1	NP_014017.1
Neurospora crassa	NCU09349	XP_958928.1
Oryza sativa (rice)	Os06g0535100	NP_001057788.2
Xenopus tropicalis (tropical clawed frog)	ddx18	NP_001016776.1
Rattus norvegicus (Norway rat)	Ddx18	NP_001006997.1
Schizosaccharomyces pombe (fission yeast)	has1	NP_594488.1
Gallus gallus (chicken)	DDX18	XP_422125.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008601	XP_314700.4
Kluyveromyces lactis	KLLA0A09669g	XP_451422.1
Magnaporthe oryzae (rice blast fungus)	MGG_06522	XP_003717029.1
Arabidopsis thaliana (thale cress)	AT5G65900	NP_201391.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pit gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pit cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05654	NM_001300530.1 Latest version!	Drosophila melanogaster pitchoune (pit), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa05654	NM_169991.2 Latest version!	Drosophila melanogaster pitchoune (pit), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa05654	NM_079722.4 Latest version!	Drosophila melanogaster pitchoune (pit), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pit ( NM_079722.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524446.3 Drosophila melanogaster pitchoune (pit), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05654
Clone ID Related Accession (Same CDS sequence)	NM_079722.4 , NM_001300530.1 , NM_169991.2
Accession Version	NM_079722.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pitchoune, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079722.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTATTC GGGAGAAGCT GCTGATGAAG AAGATCGTTA AGCGGGAGAA GATGAAGAAG 
GAGCTCTCGC AGAAGAAGGG AAACAAGAAC GCCCAAAAGC AGGAGCCACC CAAACAAAAT 
GGCAATAAAC CCAGTAAAAA ACCGGAGAAA CTCAGTAAAA AGCATGTGGC CAAGGATGAG 
GATGACGACT TAGAGGAGGA TTTCCAGGAG GCGCCGCTGC CCAAGAAGAA GCAACAAAAA 
CAGCCTCCAA AAAAGCAACA AATTCAGGTG GCCAACTCGG ATTCGGAATC AGATGACGAT 
GAGCAGGAGG ATGAGGCAGA TGAGGATAGC GATTTGGACG AAGTAGCCGA AGTTGATGAG 
GAGGATGTTG ACAGCGGGAG TGAAGATGAT GACCAGCAGG AAGATGAAGA TGAAGAAGAA 
CCTGTGCCAG CCAAGAAAAC GAAGTTGCTT CCCAACAAGT CCAAGGCACA GAATGGCAAA 
CCAGCTAAAG ACGACGAGCC CTTTACCGTG GAATCCTCAC TGGCTGCACT GGATTACCGA 
GATTCGGATG ATCGCAGCTT TGCCTCTTTA AAGGGCGCCG TGTCCGAGGC CACATTGCGA 
GCCATTAAGG AGATGGGTTT TACCGAGATG ACTGAAATTC AGTCGAAATC GCTGACACCC 
CTACTAAAGG GACGGGATCT GGTGGGTGCT GCCCAGACGG GTTCCGGCAA AACCCTGGCC 
TTCCTGATAC CCGCCGTCGA GCTGATAAAC AAGCTGAGGT TTATGCCACG CAATGGTACC 
GGCGTGATCA TCATCTCACC TACCCGAGAG TTGTCCATGC AAACTTTCGG TGTGCTCAAA 
GAGCTGATGG CACACCACCA TCACACTTAT GGCTTGGTAA TGGGCGGCTC CAATCGGCAG 
GTGGAGAGCG AGAAGTTGGG CAAGGGCATC AACATTCTGG TGGCCACACC GGGTCGTCTG 
CTGGATCATT TACAAAACTC GCCCGACTTT CTGTACAAGA ACCTTCAGTG CCTGATTATC 
GATGAAGTGG ATCGAATTCT GGAGATCGGT TTTGAGGAGG AGCTTAAGCA AATAATAAAT 
CTGCTGCCAA AACGCCGCCA GACGATGCTC TTCTCGGCCA CACAGACGGC TCGCATCGAA 
GCGCTTTCCA AGCTGGCCCT TAAGTCGGAA CCAATTTATG TAGGGGTTCA CGACAACCAG 
GACACGGCGA CCGTAGATGG ACTCGAGCAG GGGTACATTG TTTGCCCCTC GGAGAAGCGA 
CTGCTCGTGC TCTTCACGTT CCTCAAAAAG AATCGTAAGA AGAAGGTGAT GGTGTTCTTC 
TCGTCCTGCA TGTCCGTCAA ATACCACCAC GAGCTCTTCA ACTACATTGA TCTGCCAGTG 
ACCTCCATCC ACGGTAAACA AAAGCAAACG AAACGAACGA CCACCTTCTT CCAGTTCTGC 
AACGCGGAAT CTGGGATTCT CTTATGTACG GATGTGGCTG CTCGTGGATT GGACATTCCG 
CAAGTCGATT GGATTGTGCA GTACGATCCT CCAGATGATC CACGCGAGTA TATTCACAGG 
GTGGGCCGAA CGGCCAGAGG ATCGGGCACC TCGGGTCACG CCCTCCTTCT GATGCGACCG 
GAGGAGCTGG GCTTCCTGCG CTACCTTAAG GCCGCCAAGG TGCCGCTCAA TGAGTTCGAG 
TTCTCCTGGC AGAAGATAGC TGATATTCAA CTGCAGCTGG AGAAACTGAT CGCCAAGAAC 
TATTTCCTAA ACCAGTCGGC CAAGGAAGCC TTTAAGTCGT ATGTGCGCGC ATACGATTCG 
CATCAATTGA AGCAGATCTT CAATGTGAAC ACGTTGGACC TGCAGGCGGT TGCGAAAAGC 
TTTGGATTCC TTGTGCCACC CGTAGTTGAC CTGAAGGTGG GCGCGGCCAA GCGGGAGCGA 
CCGGAAAAGC GAGTGGGCGG CGGCGGTTTC GGATTCTACA AGAAAATGAA CGAGGGATCG 
GCTTCCAAGC AGCGGCACTT CAAGCAGGTC AACCGCGACC AGGCCAAGAA GTTTATGCGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524446.3
CDS	119..2161
Translation	MSIREKLLMKKIVKREKMKKELSQKKGNKNAQKQEPPKQNGNKPSKKPEKLSKKHVAKDEDDDLEEDFQEAPLPKKKQQKQPPKKQQIQVANSDSESDDDEQEDEADEDSDLDEVAEVDEEDVDSGSEDDDQQEDEDEEEPVPAKKTKLLPNKSKAQNGKPAKDDEPFTVESSLAALDYRDSDDRSFASLKGAVSEATLRAIKEMGFTEMTEIQSKSLTPLLKGRDLVGAAQTGSGKTLAFLIPAVELINKLRFMPRNGTGVIIISPTRELSMQTFGVLKELMAHHHHTYGLVMGGSNRQVESEKLGKGINILVATPGRLLDHLQNSPDFLYKNLQCLIIDEVDRILEIGFEEELKQIINLLPKRRQTMLFSATQTARIEALSKLALKSEPIYVGVHDNQDTATVDGLEQGYIVCPSEKRLLVLFTFLKKNRKKKVMVFFSSCMSVKYHHELFNYIDLPVTSIHGKQKQTKRTTTFFQFCNAESGILLCTDVAARGLDIPQVDWIVQYDPPDDPREYIHRVGRTARGSGTSGHALLLMRPEELGFLRYLKAAKVPLNEFEFSWQKIADIQLQLEKLIAKNYFLNQSAKEAFKSYVRAYDSHQLKQIFNVNTLDLQAVAKSFGFLVPPVVDLKVGAAKRERPEKRVGGGGFGFYKKMNEGSASKQRHFKQVNRDQAKKFMR

Target ORF information:

RefSeq Version	NM_079722.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pitchoune (pit), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079722.4

ORF Insert Sequence:

ATGTCTATTC GGGAGAAGCT GCTGATGAAG AAGATCGTTA AGCGGGAGAA GATGAAGAAG 
GAGCTCTCGC AGAAGAAGGG AAACAAGAAC GCCCAAAAGC AGGAGCCACC CAAACAAAAT 
GGCAATAAAC CCAGTAAAAA ACCGGAGAAA CTCAGTAAAA AGCATGTGGC CAAGGATGAG 
GATGACGACT TAGAGGAGGA TTTCCAGGAG GCGCCGCTGC CCAAGAAGAA GCAACAAAAA 
CAGCCTCCAA AAAAGCAACA AATTCAGGTG GCCAACTCGG ATTCGGAATC AGATGACGAT 
GAGCAGGAGG ATGAGGCAGA TGAGGATAGC GATTTGGACG AAGTAGCCGA AGTTGATGAG 
GAGGATGTTG ACAGCGGGAG TGAAGATGAT GACCAGCAGG AAGATGAAGA TGAAGAAGAA 
CCTGTGCCAG CCAAGAAAAC GAAGTTGCTT CCCAACAAGT CCAAGGCACA GAATGGCAAA 
CCAGCTAAAG ACGACGAGCC CTTTACCGTG GAATCCTCAC TGGCTGCACT GGATTACCGA 
GATTCGGATG ATCGCAGCTT TGCCTCTTTA AAGGGCGCCG TGTCCGAGGC CACATTGCGA 
GCCATTAAGG AGATGGGTTT TACCGAGATG ACTGAAATTC AGTCGAAATC GCTGACACCC 
CTACTAAAGG GACGGGATCT GGTGGGTGCT GCCCAGACGG GTTCCGGCAA AACCCTGGCC 
TTCCTGATAC CCGCCGTCGA GCTGATAAAC AAGCTGAGGT TTATGCCACG CAATGGTACC 
GGCGTGATCA TCATCTCACC TACCCGAGAG TTGTCCATGC AAACTTTCGG TGTGCTCAAA 
GAGCTGATGG CACACCACCA TCACACTTAT GGCTTGGTAA TGGGCGGCTC CAATCGGCAG 
GTGGAGAGCG AGAAGTTGGG CAAGGGCATC AACATTCTGG TGGCCACACC GGGTCGTCTG 
CTGGATCATT TACAAAACTC GCCCGACTTT CTGTACAAGA ACCTTCAGTG CCTGATTATC 
GATGAAGTGG ATCGAATTCT GGAGATCGGT TTTGAGGAGG AGCTTAAGCA AATAATAAAT 
CTGCTGCCAA AACGCCGCCA GACGATGCTC TTCTCGGCCA CACAGACGGC TCGCATCGAA 
GCGCTTTCCA AGCTGGCCCT TAAGTCGGAA CCAATTTATG TAGGGGTTCA CGACAACCAG 
GACACGGCGA CCGTAGATGG ACTCGAGCAG GGGTACATTG TTTGCCCCTC GGAGAAGCGA 
CTGCTCGTGC TCTTCACGTT CCTCAAAAAG AATCGTAAGA AGAAGGTGAT GGTGTTCTTC 
TCGTCCTGCA TGTCCGTCAA ATACCACCAC GAGCTCTTCA ACTACATTGA TCTGCCAGTG 
ACCTCCATCC ACGGTAAACA AAAGCAAACG AAACGAACGA CCACCTTCTT CCAGTTCTGC 
AACGCGGAAT CTGGGATTCT CTTATGTACG GATGTGGCTG CTCGTGGATT GGACATTCCG 
CAAGTCGATT GGATTGTGCA GTACGATCCT CCAGATGATC CACGCGAGTA TATTCACAGG 
GTGGGCCGAA CGGCCAGAGG ATCGGGCACC TCGGGTCACG CCCTCCTTCT GATGCGACCG 
GAGGAGCTGG GCTTCCTGCG CTACCTTAAG GCCGCCAAGG TGCCGCTCAA TGAGTTCGAG 
TTCTCCTGGC AGAAGATAGC TGATATTCAA CTGCAGCTGG AGAAACTGAT CGCCAAGAAC 
TATTTCCTAA ACCAGTCGGC CAAGGAAGCC TTTAAGTCGT ATGTGCGCGC ATACGATTCG 
CATCAATTGA AGCAGATCTT CAATGTGAAC ACGTTGGACC TGCAGGCGGT TGCGAAAAGC 
TTTGGATTCC TTGTGCCACC CGTAGTTGAC CTGAAGGTGG GCGCGGCCAA GCGGGAGCGA 
CCGGAAAAGC GAGTGGGCGG CGGCGGTTTC GGATTCTACA AGAAAATGAA CGAGGGATCG 
GCTTCCAAGC AGCGGCACTT CAAGCAGGTC AACCGCGACC AGGCCAAGAA GTTTATGCGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pit ( NM_001300530.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287459.1 Drosophila melanogaster pitchoune (pit), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05654
Clone ID Related Accession (Same CDS sequence)	NM_079722.4 , NM_001300530.1 , NM_169991.2
Accession Version	NM_001300530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pitchoune, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTATTC GGGAGAAGCT GCTGATGAAG AAGATCGTTA AGCGGGAGAA GATGAAGAAG 
GAGCTCTCGC AGAAGAAGGG AAACAAGAAC GCCCAAAAGC AGGAGCCACC CAAACAAAAT 
GGCAATAAAC CCAGTAAAAA ACCGGAGAAA CTCAGTAAAA AGCATGTGGC CAAGGATGAG 
GATGACGACT TAGAGGAGGA TTTCCAGGAG GCGCCGCTGC CCAAGAAGAA GCAACAAAAA 
CAGCCTCCAA AAAAGCAACA AATTCAGGTG GCCAACTCGG ATTCGGAATC AGATGACGAT 
GAGCAGGAGG ATGAGGCAGA TGAGGATAGC GATTTGGACG AAGTAGCCGA AGTTGATGAG 
GAGGATGTTG ACAGCGGGAG TGAAGATGAT GACCAGCAGG AAGATGAAGA TGAAGAAGAA 
CCTGTGCCAG CCAAGAAAAC GAAGTTGCTT CCCAACAAGT CCAAGGCACA GAATGGCAAA 
CCAGCTAAAG ACGACGAGCC CTTTACCGTG GAATCCTCAC TGGCTGCACT GGATTACCGA 
GATTCGGATG ATCGCAGCTT TGCCTCTTTA AAGGGCGCCG TGTCCGAGGC CACATTGCGA 
GCCATTAAGG AGATGGGTTT TACCGAGATG ACTGAAATTC AGTCGAAATC GCTGACACCC 
CTACTAAAGG GACGGGATCT GGTGGGTGCT GCCCAGACGG GTTCCGGCAA AACCCTGGCC 
TTCCTGATAC CCGCCGTCGA GCTGATAAAC AAGCTGAGGT TTATGCCACG CAATGGTACC 
GGCGTGATCA TCATCTCACC TACCCGAGAG TTGTCCATGC AAACTTTCGG TGTGCTCAAA 
GAGCTGATGG CACACCACCA TCACACTTAT GGCTTGGTAA TGGGCGGCTC CAATCGGCAG 
GTGGAGAGCG AGAAGTTGGG CAAGGGCATC AACATTCTGG TGGCCACACC GGGTCGTCTG 
CTGGATCATT TACAAAACTC GCCCGACTTT CTGTACAAGA ACCTTCAGTG CCTGATTATC 
GATGAAGTGG ATCGAATTCT GGAGATCGGT TTTGAGGAGG AGCTTAAGCA AATAATAAAT 
CTGCTGCCAA AACGCCGCCA GACGATGCTC TTCTCGGCCA CACAGACGGC TCGCATCGAA 
GCGCTTTCCA AGCTGGCCCT TAAGTCGGAA CCAATTTATG TAGGGGTTCA CGACAACCAG 
GACACGGCGA CCGTAGATGG ACTCGAGCAG GGGTACATTG TTTGCCCCTC GGAGAAGCGA 
CTGCTCGTGC TCTTCACGTT CCTCAAAAAG AATCGTAAGA AGAAGGTGAT GGTGTTCTTC 
TCGTCCTGCA TGTCCGTCAA ATACCACCAC GAGCTCTTCA ACTACATTGA TCTGCCAGTG 
ACCTCCATCC ACGGTAAACA AAAGCAAACG AAACGAACGA CCACCTTCTT CCAGTTCTGC 
AACGCGGAAT CTGGGATTCT CTTATGTACG GATGTGGCTG CTCGTGGATT GGACATTCCG 
CAAGTCGATT GGATTGTGCA GTACGATCCT CCAGATGATC CACGCGAGTA TATTCACAGG 
GTGGGCCGAA CGGCCAGAGG ATCGGGCACC TCGGGTCACG CCCTCCTTCT GATGCGACCG 
GAGGAGCTGG GCTTCCTGCG CTACCTTAAG GCCGCCAAGG TGCCGCTCAA TGAGTTCGAG 
TTCTCCTGGC AGAAGATAGC TGATATTCAA CTGCAGCTGG AGAAACTGAT CGCCAAGAAC 
TATTTCCTAA ACCAGTCGGC CAAGGAAGCC TTTAAGTCGT ATGTGCGCGC ATACGATTCG 
CATCAATTGA AGCAGATCTT CAATGTGAAC ACGTTGGACC TGCAGGCGGT TGCGAAAAGC 
TTTGGATTCC TTGTGCCACC CGTAGTTGAC CTGAAGGTGG GCGCGGCCAA GCGGGAGCGA 
CCGGAAAAGC GAGTGGGCGG CGGCGGTTTC GGATTCTACA AGAAAATGAA CGAGGGATCG 
GCTTCCAAGC AGCGGCACTT CAAGCAGGTC AACCGCGACC AGGCCAAGAA GTTTATGCGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287459.1
CDS	119..2161
Translation	MSIREKLLMKKIVKREKMKKELSQKKGNKNAQKQEPPKQNGNKPSKKPEKLSKKHVAKDEDDDLEEDFQEAPLPKKKQQKQPPKKQQIQVANSDSESDDDEQEDEADEDSDLDEVAEVDEEDVDSGSEDDDQQEDEDEEEPVPAKKTKLLPNKSKAQNGKPAKDDEPFTVESSLAALDYRDSDDRSFASLKGAVSEATLRAIKEMGFTEMTEIQSKSLTPLLKGRDLVGAAQTGSGKTLAFLIPAVELINKLRFMPRNGTGVIIISPTRELSMQTFGVLKELMAHHHHTYGLVMGGSNRQVESEKLGKGINILVATPGRLLDHLQNSPDFLYKNLQCLIIDEVDRILEIGFEEELKQIINLLPKRRQTMLFSATQTARIEALSKLALKSEPIYVGVHDNQDTATVDGLEQGYIVCPSEKRLLVLFTFLKKNRKKKVMVFFSSCMSVKYHHELFNYIDLPVTSIHGKQKQTKRTTTFFQFCNAESGILLCTDVAARGLDIPQVDWIVQYDPPDDPREYIHRVGRTARGSGTSGHALLLMRPEELGFLRYLKAAKVPLNEFEFSWQKIADIQLQLEKLIAKNYFLNQSAKEAFKSYVRAYDSHQLKQIFNVNTLDLQAVAKSFGFLVPPVVDLKVGAAKRERPEKRVGGGGFGFYKKMNEGSASKQRHFKQVNRDQAKKFMR

Target ORF information:

RefSeq Version	NM_001300530.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pitchoune (pit), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300530.1

ORF Insert Sequence:

ATGTCTATTC GGGAGAAGCT GCTGATGAAG AAGATCGTTA AGCGGGAGAA GATGAAGAAG 
GAGCTCTCGC AGAAGAAGGG AAACAAGAAC GCCCAAAAGC AGGAGCCACC CAAACAAAAT 
GGCAATAAAC CCAGTAAAAA ACCGGAGAAA CTCAGTAAAA AGCATGTGGC CAAGGATGAG 
GATGACGACT TAGAGGAGGA TTTCCAGGAG GCGCCGCTGC CCAAGAAGAA GCAACAAAAA 
CAGCCTCCAA AAAAGCAACA AATTCAGGTG GCCAACTCGG ATTCGGAATC AGATGACGAT 
GAGCAGGAGG ATGAGGCAGA TGAGGATAGC GATTTGGACG AAGTAGCCGA AGTTGATGAG 
GAGGATGTTG ACAGCGGGAG TGAAGATGAT GACCAGCAGG AAGATGAAGA TGAAGAAGAA 
CCTGTGCCAG CCAAGAAAAC GAAGTTGCTT CCCAACAAGT CCAAGGCACA GAATGGCAAA 
CCAGCTAAAG ACGACGAGCC CTTTACCGTG GAATCCTCAC TGGCTGCACT GGATTACCGA 
GATTCGGATG ATCGCAGCTT TGCCTCTTTA AAGGGCGCCG TGTCCGAGGC CACATTGCGA 
GCCATTAAGG AGATGGGTTT TACCGAGATG ACTGAAATTC AGTCGAAATC GCTGACACCC 
CTACTAAAGG GACGGGATCT GGTGGGTGCT GCCCAGACGG GTTCCGGCAA AACCCTGGCC 
TTCCTGATAC CCGCCGTCGA GCTGATAAAC AAGCTGAGGT TTATGCCACG CAATGGTACC 
GGCGTGATCA TCATCTCACC TACCCGAGAG TTGTCCATGC AAACTTTCGG TGTGCTCAAA 
GAGCTGATGG CACACCACCA TCACACTTAT GGCTTGGTAA TGGGCGGCTC CAATCGGCAG 
GTGGAGAGCG AGAAGTTGGG CAAGGGCATC AACATTCTGG TGGCCACACC GGGTCGTCTG 
CTGGATCATT TACAAAACTC GCCCGACTTT CTGTACAAGA ACCTTCAGTG CCTGATTATC 
GATGAAGTGG ATCGAATTCT GGAGATCGGT TTTGAGGAGG AGCTTAAGCA AATAATAAAT 
CTGCTGCCAA AACGCCGCCA GACGATGCTC TTCTCGGCCA CACAGACGGC TCGCATCGAA 
GCGCTTTCCA AGCTGGCCCT TAAGTCGGAA CCAATTTATG TAGGGGTTCA CGACAACCAG 
GACACGGCGA CCGTAGATGG ACTCGAGCAG GGGTACATTG TTTGCCCCTC GGAGAAGCGA 
CTGCTCGTGC TCTTCACGTT CCTCAAAAAG AATCGTAAGA AGAAGGTGAT GGTGTTCTTC 
TCGTCCTGCA TGTCCGTCAA ATACCACCAC GAGCTCTTCA ACTACATTGA TCTGCCAGTG 
ACCTCCATCC ACGGTAAACA AAAGCAAACG AAACGAACGA CCACCTTCTT CCAGTTCTGC 
AACGCGGAAT CTGGGATTCT CTTATGTACG GATGTGGCTG CTCGTGGATT GGACATTCCG 
CAAGTCGATT GGATTGTGCA GTACGATCCT CCAGATGATC CACGCGAGTA TATTCACAGG 
GTGGGCCGAA CGGCCAGAGG ATCGGGCACC TCGGGTCACG CCCTCCTTCT GATGCGACCG 
GAGGAGCTGG GCTTCCTGCG CTACCTTAAG GCCGCCAAGG TGCCGCTCAA TGAGTTCGAG 
TTCTCCTGGC AGAAGATAGC TGATATTCAA CTGCAGCTGG AGAAACTGAT CGCCAAGAAC 
TATTTCCTAA ACCAGTCGGC CAAGGAAGCC TTTAAGTCGT ATGTGCGCGC ATACGATTCG 
CATCAATTGA AGCAGATCTT CAATGTGAAC ACGTTGGACC TGCAGGCGGT TGCGAAAAGC 
TTTGGATTCC TTGTGCCACC CGTAGTTGAC CTGAAGGTGG GCGCGGCCAA GCGGGAGCGA 
CCGGAAAAGC GAGTGGGCGG CGGCGGTTTC GGATTCTACA AGAAAATGAA CGAGGGATCG 
GCTTCCAAGC AGCGGCACTT CAAGCAGGTC AACCGCGACC AGGCCAAGAA GTTTATGCGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pit ( NM_169991.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732694.2 Drosophila melanogaster pitchoune (pit), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05654
Clone ID Related Accession (Same CDS sequence)	NM_079722.4 , NM_001300530.1 , NM_169991.2
Accession Version	NM_169991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pitchoune, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_169991.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTATTC GGGAGAAGCT GCTGATGAAG AAGATCGTTA AGCGGGAGAA GATGAAGAAG 
GAGCTCTCGC AGAAGAAGGG AAACAAGAAC GCCCAAAAGC AGGAGCCACC CAAACAAAAT 
GGCAATAAAC CCAGTAAAAA ACCGGAGAAA CTCAGTAAAA AGCATGTGGC CAAGGATGAG 
GATGACGACT TAGAGGAGGA TTTCCAGGAG GCGCCGCTGC CCAAGAAGAA GCAACAAAAA 
CAGCCTCCAA AAAAGCAACA AATTCAGGTG GCCAACTCGG ATTCGGAATC AGATGACGAT 
GAGCAGGAGG ATGAGGCAGA TGAGGATAGC GATTTGGACG AAGTAGCCGA AGTTGATGAG 
GAGGATGTTG ACAGCGGGAG TGAAGATGAT GACCAGCAGG AAGATGAAGA TGAAGAAGAA 
CCTGTGCCAG CCAAGAAAAC GAAGTTGCTT CCCAACAAGT CCAAGGCACA GAATGGCAAA 
CCAGCTAAAG ACGACGAGCC CTTTACCGTG GAATCCTCAC TGGCTGCACT GGATTACCGA 
GATTCGGATG ATCGCAGCTT TGCCTCTTTA AAGGGCGCCG TGTCCGAGGC CACATTGCGA 
GCCATTAAGG AGATGGGTTT TACCGAGATG ACTGAAATTC AGTCGAAATC GCTGACACCC 
CTACTAAAGG GACGGGATCT GGTGGGTGCT GCCCAGACGG GTTCCGGCAA AACCCTGGCC 
TTCCTGATAC CCGCCGTCGA GCTGATAAAC AAGCTGAGGT TTATGCCACG CAATGGTACC 
GGCGTGATCA TCATCTCACC TACCCGAGAG TTGTCCATGC AAACTTTCGG TGTGCTCAAA 
GAGCTGATGG CACACCACCA TCACACTTAT GGCTTGGTAA TGGGCGGCTC CAATCGGCAG 
GTGGAGAGCG AGAAGTTGGG CAAGGGCATC AACATTCTGG TGGCCACACC GGGTCGTCTG 
CTGGATCATT TACAAAACTC GCCCGACTTT CTGTACAAGA ACCTTCAGTG CCTGATTATC 
GATGAAGTGG ATCGAATTCT GGAGATCGGT TTTGAGGAGG AGCTTAAGCA AATAATAAAT 
CTGCTGCCAA AACGCCGCCA GACGATGCTC TTCTCGGCCA CACAGACGGC TCGCATCGAA 
GCGCTTTCCA AGCTGGCCCT TAAGTCGGAA CCAATTTATG TAGGGGTTCA CGACAACCAG 
GACACGGCGA CCGTAGATGG ACTCGAGCAG GGGTACATTG TTTGCCCCTC GGAGAAGCGA 
CTGCTCGTGC TCTTCACGTT CCTCAAAAAG AATCGTAAGA AGAAGGTGAT GGTGTTCTTC 
TCGTCCTGCA TGTCCGTCAA ATACCACCAC GAGCTCTTCA ACTACATTGA TCTGCCAGTG 
ACCTCCATCC ACGGTAAACA AAAGCAAACG AAACGAACGA CCACCTTCTT CCAGTTCTGC 
AACGCGGAAT CTGGGATTCT CTTATGTACG GATGTGGCTG CTCGTGGATT GGACATTCCG 
CAAGTCGATT GGATTGTGCA GTACGATCCT CCAGATGATC CACGCGAGTA TATTCACAGG 
GTGGGCCGAA CGGCCAGAGG ATCGGGCACC TCGGGTCACG CCCTCCTTCT GATGCGACCG 
GAGGAGCTGG GCTTCCTGCG CTACCTTAAG GCCGCCAAGG TGCCGCTCAA TGAGTTCGAG 
TTCTCCTGGC AGAAGATAGC TGATATTCAA CTGCAGCTGG AGAAACTGAT CGCCAAGAAC 
TATTTCCTAA ACCAGTCGGC CAAGGAAGCC TTTAAGTCGT ATGTGCGCGC ATACGATTCG 
CATCAATTGA AGCAGATCTT CAATGTGAAC ACGTTGGACC TGCAGGCGGT TGCGAAAAGC 
TTTGGATTCC TTGTGCCACC CGTAGTTGAC CTGAAGGTGG GCGCGGCCAA GCGGGAGCGA 
CCGGAAAAGC GAGTGGGCGG CGGCGGTTTC GGATTCTACA AGAAAATGAA CGAGGGATCG 
GCTTCCAAGC AGCGGCACTT CAAGCAGGTC AACCGCGACC AGGCCAAGAA GTTTATGCGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732694.2
CDS	173..2215
Translation	MSIREKLLMKKIVKREKMKKELSQKKGNKNAQKQEPPKQNGNKPSKKPEKLSKKHVAKDEDDDLEEDFQEAPLPKKKQQKQPPKKQQIQVANSDSESDDDEQEDEADEDSDLDEVAEVDEEDVDSGSEDDDQQEDEDEEEPVPAKKTKLLPNKSKAQNGKPAKDDEPFTVESSLAALDYRDSDDRSFASLKGAVSEATLRAIKEMGFTEMTEIQSKSLTPLLKGRDLVGAAQTGSGKTLAFLIPAVELINKLRFMPRNGTGVIIISPTRELSMQTFGVLKELMAHHHHTYGLVMGGSNRQVESEKLGKGINILVATPGRLLDHLQNSPDFLYKNLQCLIIDEVDRILEIGFEEELKQIINLLPKRRQTMLFSATQTARIEALSKLALKSEPIYVGVHDNQDTATVDGLEQGYIVCPSEKRLLVLFTFLKKNRKKKVMVFFSSCMSVKYHHELFNYIDLPVTSIHGKQKQTKRTTTFFQFCNAESGILLCTDVAARGLDIPQVDWIVQYDPPDDPREYIHRVGRTARGSGTSGHALLLMRPEELGFLRYLKAAKVPLNEFEFSWQKIADIQLQLEKLIAKNYFLNQSAKEAFKSYVRAYDSHQLKQIFNVNTLDLQAVAKSFGFLVPPVVDLKVGAAKRERPEKRVGGGGFGFYKKMNEGSASKQRHFKQVNRDQAKKFMR

Target ORF information:

RefSeq Version	NM_169991.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pitchoune (pit), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169991.2

ORF Insert Sequence:

ATGTCTATTC GGGAGAAGCT GCTGATGAAG AAGATCGTTA AGCGGGAGAA GATGAAGAAG 
GAGCTCTCGC AGAAGAAGGG AAACAAGAAC GCCCAAAAGC AGGAGCCACC CAAACAAAAT 
GGCAATAAAC CCAGTAAAAA ACCGGAGAAA CTCAGTAAAA AGCATGTGGC CAAGGATGAG 
GATGACGACT TAGAGGAGGA TTTCCAGGAG GCGCCGCTGC CCAAGAAGAA GCAACAAAAA 
CAGCCTCCAA AAAAGCAACA AATTCAGGTG GCCAACTCGG ATTCGGAATC AGATGACGAT 
GAGCAGGAGG ATGAGGCAGA TGAGGATAGC GATTTGGACG AAGTAGCCGA AGTTGATGAG 
GAGGATGTTG ACAGCGGGAG TGAAGATGAT GACCAGCAGG AAGATGAAGA TGAAGAAGAA 
CCTGTGCCAG CCAAGAAAAC GAAGTTGCTT CCCAACAAGT CCAAGGCACA GAATGGCAAA 
CCAGCTAAAG ACGACGAGCC CTTTACCGTG GAATCCTCAC TGGCTGCACT GGATTACCGA 
GATTCGGATG ATCGCAGCTT TGCCTCTTTA AAGGGCGCCG TGTCCGAGGC CACATTGCGA 
GCCATTAAGG AGATGGGTTT TACCGAGATG ACTGAAATTC AGTCGAAATC GCTGACACCC 
CTACTAAAGG GACGGGATCT GGTGGGTGCT GCCCAGACGG GTTCCGGCAA AACCCTGGCC 
TTCCTGATAC CCGCCGTCGA GCTGATAAAC AAGCTGAGGT TTATGCCACG CAATGGTACC 
GGCGTGATCA TCATCTCACC TACCCGAGAG TTGTCCATGC AAACTTTCGG TGTGCTCAAA 
GAGCTGATGG CACACCACCA TCACACTTAT GGCTTGGTAA TGGGCGGCTC CAATCGGCAG 
GTGGAGAGCG AGAAGTTGGG CAAGGGCATC AACATTCTGG TGGCCACACC GGGTCGTCTG 
CTGGATCATT TACAAAACTC GCCCGACTTT CTGTACAAGA ACCTTCAGTG CCTGATTATC 
GATGAAGTGG ATCGAATTCT GGAGATCGGT TTTGAGGAGG AGCTTAAGCA AATAATAAAT 
CTGCTGCCAA AACGCCGCCA GACGATGCTC TTCTCGGCCA CACAGACGGC TCGCATCGAA 
GCGCTTTCCA AGCTGGCCCT TAAGTCGGAA CCAATTTATG TAGGGGTTCA CGACAACCAG 
GACACGGCGA CCGTAGATGG ACTCGAGCAG GGGTACATTG TTTGCCCCTC GGAGAAGCGA 
CTGCTCGTGC TCTTCACGTT CCTCAAAAAG AATCGTAAGA AGAAGGTGAT GGTGTTCTTC 
TCGTCCTGCA TGTCCGTCAA ATACCACCAC GAGCTCTTCA ACTACATTGA TCTGCCAGTG 
ACCTCCATCC ACGGTAAACA AAAGCAAACG AAACGAACGA CCACCTTCTT CCAGTTCTGC 
AACGCGGAAT CTGGGATTCT CTTATGTACG GATGTGGCTG CTCGTGGATT GGACATTCCG 
CAAGTCGATT GGATTGTGCA GTACGATCCT CCAGATGATC CACGCGAGTA TATTCACAGG 
GTGGGCCGAA CGGCCAGAGG ATCGGGCACC TCGGGTCACG CCCTCCTTCT GATGCGACCG 
GAGGAGCTGG GCTTCCTGCG CTACCTTAAG GCCGCCAAGG TGCCGCTCAA TGAGTTCGAG 
TTCTCCTGGC AGAAGATAGC TGATATTCAA CTGCAGCTGG AGAAACTGAT CGCCAAGAAC 
TATTTCCTAA ACCAGTCGGC CAAGGAAGCC TTTAAGTCGT ATGTGCGCGC ATACGATTCG 
CATCAATTGA AGCAGATCTT CAATGTGAAC ACGTTGGACC TGCAGGCGGT TGCGAAAAGC 
TTTGGATTCC TTGTGCCACC CGTAGTTGAC CTGAAGGTGG GCGCGGCCAA GCGGGAGCGA 
CCGGAAAAGC GAGTGGGCGG CGGCGGTTTC GGATTCTACA AGAAAATGAA CGAGGGATCG 
GCTTCCAAGC AGCGGCACTT CAAGCAGGTC AACCGCGACC AGGCCAAGAA GTTTATGCGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pit cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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