ND-42 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ND-42
Entrez Gene ID	42591
Full Name	NADH dehydrogenase (ubiquinone) 42 kDa subunit
Synonyms	A10,CG6343,Dmel\CG6343,ND42,NUDM,NdufA10
General protein information	Preferred Names NADH dehydrogenase (ubiquinone) 42 kDa subunit Names CG6343 gene product from transcript CG6343-RB CG6343-PA CG6343-PB CG6343-PC NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10 NADH ubiquinone oxidoreductase 42 kDa NADH-ubiquinone oxidoreductase 42 kDa subunit NADH-ubiquinone oxidoreductase subunit NADH: UBIQUINONE REDUCTASE 42 KD SUBUNIT PRECURSOR NADH:ubiquinone oxidoreductase 42 kDa subunit NADH:ubiquinone reductase 42 kD NADH:ubiquinone reductase 42kD subunit precursor ND-42-PA ND-42-PB ND-42-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94A1-94A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_169990.3	NP_732692.1	NADH dehydrogenase (ubiquinone) 42 kDa subunit, isoform B
NM_079721.4	NP_524445.1	NADH dehydrogenase (ubiquinone) 42 kDa subunit, isoform A
NM_001275892.1	NP_001262821.1	NADH dehydrogenase (ubiquinone) 42 kDa subunit, isoform C

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ndufa10	NP_955789.2
Drosophila melanogaster (fruit fly)	ND42	NP_001262821.1
	NDUFA10	XP_001087784.2
Canis lupus familiaris (dog)	LOC608244	XP_005635987.1
Mus musculus (house mouse)	Ndufa10	NP_077159.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011130	XP_309517.4
Caenorhabditis elegans (roundworm)	nuo-4	NP_741215.1
Xenopus tropicalis (tropical clawed frog)	ndufa10	NP_001076817.1
Homo sapiens (human)	NDUFA10	NP_004535.1
Bos taurus (cattle)	NDUFA10	NP_788828.1
Gallus gallus (chicken)	NDUFA10	NP_001026418.1
Pan troglodytes (chimpanzee)	NDUFA10	NP_001065273.1
Rattus norvegicus (Norway rat)	Ndufa10l1	NP_872612.1
Danio rerio (zebrafish)	ndufa10	NP_955872.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings support the emerging view that PINK1 plays a role in regulating complex I activity separate from its role with Parkin in mitophagy
Title: The complex I subunit NDUFA10 selectively rescues Drosophila pink1 mutants through a mechanism independent of mitophagy.

The following ND-42 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ND-42 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05650	NM_001275892.1 Latest version!	Drosophila melanogaster NADH dehydrogenase (ubiquinone) 42 kDa subunit (ND-42), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa05650	NM_079721.4 Latest version!	Drosophila melanogaster NADH dehydrogenase (ubiquinone) 42 kDa subunit (ND-42), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa05650	NM_169990.3 Latest version!	Drosophila melanogaster NADH dehydrogenase (ubiquinone) 42 kDa subunit (ND-42), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ND-42 ( NM_169990.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732692.1 Drosophila melanogaster NADH dehydrogenase (ubiquinone) 42 kDa subunit (ND-42), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05650
Clone ID Related Accession (Same CDS sequence)	NM_169990.3 , NM_079721.4 , NM_001275892.1
Accession Version	NM_169990.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NADH dehydrogenase (ubiquinone) 42 kDa subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169990.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGCCG TGTTCCGCGT AGGACTGGTG CGGCTCGTCA GCCGCGCCAC ACAGTCGCCC 
AATCTACTCC AGGCGCAGAC GAATGCCCTG CCTGCTGCCT TCCAGCAGCG ATGCAGCATC 
TCCGGCAAGA CGATGCGAGG TGGACCACGG GTGCCCAAGG CCGCCCCCTA TCCGTACAAG 
ACGAAGAAGT ACAGCGTGTT CAATGCTATT TTCGACAAGA CCTCCAAGCG TTTTGACGAG 
AACTCCAAGG TGATCTGTGT GGAGGGACCC ATCGCGGCCG GCAAGTCAAA GTTCGCCAAG 
GAACTGGCTG AGGAGCTGGA CATGGAGTAC TATCCGGCAG TTGATCTGGA CCTAATCTAC 
ATCAATTCGT ACGGCTATGA CATGAGGAAG TTGGATCCTC AGTTGCCGCC CAGCTGCCGA 
AGCTACGATG TGCGCAACTT CTGCCTGGAT CCCAGCCACG ATCTGGCTGC CCAGTTCCAG 
ATTCGCATGT ACATGCTGCG CTATTCGCAG TACATTGATG CCCTGCAGCA CGTCCTTAGC 
ACCGGCCAGG GTGTTGTCCT CGAGCGCAGC CCCTACTCGG ACTTTGTCTT CATGGAGGCC 
ATGTTTCGGC AGGGTTATCT GTCACGCGGT GCGCGTTCCG TGTATAATGA GCTCCGACAG 
AACACCATCG GGGAGCTGCT GAAGCCGCAT TTGGTTATCT ACTTGGATCT GCCGGTCGAT 
GCCGTGAAGA AGCAGATCAA GGCACGCAAT GTGGACTACG AGGTGCAGTC AAAGGTGTTC 
AGCGATGCCT ACTTGAGCGA TTTGGAGCAG CTGTACAAGC AGCAGTACCT CAAGGACATC 
TCCACCCATG CCGAGCTGCT CATCTACGAC TGGACAGCCG GCGGTGAGAC TGAGGTTGTG 
GTGGAGGATA TCGAACGCAT CGACTTCAAC CAGTTTGAGG CCGATATTCA CAACAAGAAG 
ATGCTCGACT GGCGTTTCCC GCTGGAGGCC GAGTGGTGCG AAGCCCGTAT CAAGTACTGC 
CATGAGAAGC CCGATCTGAT GAATTACTTC AATGTGCCGC GTTTCGATGT CCCGGAGCTG 
GTGCGCAGCG CTGACGACGG CAAAGTCTGG CGTGATGTCT GGTTCAATGC TCCCGGCATG 
AAGTACCGTC CTGGCTACAA TGCAGACATG GGCGACGAGG GTCTCCTCAC CAAGACGAAA 
ATAGGCATCA ACCAGGGCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732692.1
CDS	313..1536
Translation	MTAVFRVGLVRLVSRATQSPNLLQAQTNALPAAFQQRCSISGKTMRGGPRVPKAAPYPYKTKKYSVFNAIFDKTSKRFDENSKVICVEGPIAAGKSKFAKELAEELDMEYYPAVDLDLIYINSYGYDMRKLDPQLPPSCRSYDVRNFCLDPSHDLAAQFQIRMYMLRYSQYIDALQHVLSTGQGVVLERSPYSDFVFMEAMFRQGYLSRGARSVYNELRQNTIGELLKPHLVIYLDLPVDAVKKQIKARNVDYEVQSKVFSDAYLSDLEQLYKQQYLKDISTHAELLIYDWTAGGETEVVVEDIERIDFNQFEADIHNKKMLDWRFPLEAEWCEARIKYCHEKPDLMNYFNVPRFDVPELVRSADDGKVWRDVWFNAPGMKYRPGYNADMGDEGLLTKTKIGINQGI

Target ORF information:

RefSeq Version	NM_169990.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NADH dehydrogenase (ubiquinone) 42 kDa subunit (ND-42), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169990.3

ORF Insert Sequence:

ATGACCGCCG TGTTCCGCGT AGGACTGGTG CGGCTCGTCA GCCGCGCCAC ACAGTCGCCC 
AATCTACTCC AGGCGCAGAC GAATGCCCTG CCTGCTGCCT TCCAGCAGCG ATGCAGCATC 
TCCGGCAAGA CGATGCGAGG TGGACCACGG GTGCCCAAGG CCGCCCCCTA TCCGTACAAG 
ACGAAGAAGT ACAGCGTGTT CAATGCTATT TTCGACAAGA CCTCCAAGCG TTTTGACGAG 
AACTCCAAGG TGATCTGTGT GGAGGGACCC ATCGCGGCCG GCAAGTCAAA GTTCGCCAAG 
GAACTGGCTG AGGAGCTGGA CATGGAGTAC TATCCGGCAG TTGATCTGGA CCTAATCTAC 
ATCAATTCGT ACGGCTATGA CATGAGGAAG TTGGATCCTC AGTTGCCGCC CAGCTGCCGA 
AGCTACGATG TGCGCAACTT CTGCCTGGAT CCCAGCCACG ATCTGGCTGC CCAGTTCCAG 
ATTCGCATGT ACATGCTGCG CTATTCGCAG TACATTGATG CCCTGCAGCA CGTCCTTAGC 
ACCGGCCAGG GTGTTGTCCT CGAGCGCAGC CCCTACTCGG ACTTTGTCTT CATGGAGGCC 
ATGTTTCGGC AGGGTTATCT GTCACGCGGT GCGCGTTCCG TGTATAATGA GCTCCGACAG 
AACACCATCG GGGAGCTGCT GAAGCCGCAT TTGGTTATCT ACTTGGATCT GCCGGTCGAT 
GCCGTGAAGA AGCAGATCAA GGCACGCAAT GTGGACTACG AGGTGCAGTC AAAGGTGTTC 
AGCGATGCCT ACTTGAGCGA TTTGGAGCAG CTGTACAAGC AGCAGTACCT CAAGGACATC 
TCCACCCATG CCGAGCTGCT CATCTACGAC TGGACAGCCG GCGGTGAGAC TGAGGTTGTG 
GTGGAGGATA TCGAACGCAT CGACTTCAAC CAGTTTGAGG CCGATATTCA CAACAAGAAG 
ATGCTCGACT GGCGTTTCCC GCTGGAGGCC GAGTGGTGCG AAGCCCGTAT CAAGTACTGC 
CATGAGAAGC CCGATCTGAT GAATTACTTC AATGTGCCGC GTTTCGATGT CCCGGAGCTG 
GTGCGCAGCG CTGACGACGG CAAAGTCTGG CGTGATGTCT GGTTCAATGC TCCCGGCATG 
AAGTACCGTC CTGGCTACAA TGCAGACATG GGCGACGAGG GTCTCCTCAC CAAGACGAAA 
ATAGGCATCA ACCAGGGCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ND-42 ( NM_079721.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524445.1 Drosophila melanogaster NADH dehydrogenase (ubiquinone) 42 kDa subunit (ND-42), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05650
Clone ID Related Accession (Same CDS sequence)	NM_169990.3 , NM_079721.4 , NM_001275892.1
Accession Version	NM_079721.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NADH dehydrogenase (ubiquinone) 42 kDa subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079721.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGCCG TGTTCCGCGT AGGACTGGTG CGGCTCGTCA GCCGCGCCAC ACAGTCGCCC 
AATCTACTCC AGGCGCAGAC GAATGCCCTG CCTGCTGCCT TCCAGCAGCG ATGCAGCATC 
TCCGGCAAGA CGATGCGAGG TGGACCACGG GTGCCCAAGG CCGCCCCCTA TCCGTACAAG 
ACGAAGAAGT ACAGCGTGTT CAATGCTATT TTCGACAAGA CCTCCAAGCG TTTTGACGAG 
AACTCCAAGG TGATCTGTGT GGAGGGACCC ATCGCGGCCG GCAAGTCAAA GTTCGCCAAG 
GAACTGGCTG AGGAGCTGGA CATGGAGTAC TATCCGGCAG TTGATCTGGA CCTAATCTAC 
ATCAATTCGT ACGGCTATGA CATGAGGAAG TTGGATCCTC AGTTGCCGCC CAGCTGCCGA 
AGCTACGATG TGCGCAACTT CTGCCTGGAT CCCAGCCACG ATCTGGCTGC CCAGTTCCAG 
ATTCGCATGT ACATGCTGCG CTATTCGCAG TACATTGATG CCCTGCAGCA CGTCCTTAGC 
ACCGGCCAGG GTGTTGTCCT CGAGCGCAGC CCCTACTCGG ACTTTGTCTT CATGGAGGCC 
ATGTTTCGGC AGGGTTATCT GTCACGCGGT GCGCGTTCCG TGTATAATGA GCTCCGACAG 
AACACCATCG GGGAGCTGCT GAAGCCGCAT TTGGTTATCT ACTTGGATCT GCCGGTCGAT 
GCCGTGAAGA AGCAGATCAA GGCACGCAAT GTGGACTACG AGGTGCAGTC AAAGGTGTTC 
AGCGATGCCT ACTTGAGCGA TTTGGAGCAG CTGTACAAGC AGCAGTACCT CAAGGACATC 
TCCACCCATG CCGAGCTGCT CATCTACGAC TGGACAGCCG GCGGTGAGAC TGAGGTTGTG 
GTGGAGGATA TCGAACGCAT CGACTTCAAC CAGTTTGAGG CCGATATTCA CAACAAGAAG 
ATGCTCGACT GGCGTTTCCC GCTGGAGGCC GAGTGGTGCG AAGCCCGTAT CAAGTACTGC 
CATGAGAAGC CCGATCTGAT GAATTACTTC AATGTGCCGC GTTTCGATGT CCCGGAGCTG 
GTGCGCAGCG CTGACGACGG CAAAGTCTGG CGTGATGTCT GGTTCAATGC TCCCGGCATG 
AAGTACCGTC CTGGCTACAA TGCAGACATG GGCGACGAGG GTCTCCTCAC CAAGACGAAA 
ATAGGCATCA ACCAGGGCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524445.1
CDS	74..1297
Translation	MTAVFRVGLVRLVSRATQSPNLLQAQTNALPAAFQQRCSISGKTMRGGPRVPKAAPYPYKTKKYSVFNAIFDKTSKRFDENSKVICVEGPIAAGKSKFAKELAEELDMEYYPAVDLDLIYINSYGYDMRKLDPQLPPSCRSYDVRNFCLDPSHDLAAQFQIRMYMLRYSQYIDALQHVLSTGQGVVLERSPYSDFVFMEAMFRQGYLSRGARSVYNELRQNTIGELLKPHLVIYLDLPVDAVKKQIKARNVDYEVQSKVFSDAYLSDLEQLYKQQYLKDISTHAELLIYDWTAGGETEVVVEDIERIDFNQFEADIHNKKMLDWRFPLEAEWCEARIKYCHEKPDLMNYFNVPRFDVPELVRSADDGKVWRDVWFNAPGMKYRPGYNADMGDEGLLTKTKIGINQGI

Target ORF information:

RefSeq Version	NM_079721.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NADH dehydrogenase (ubiquinone) 42 kDa subunit (ND-42), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079721.4

ORF Insert Sequence:

ATGACCGCCG TGTTCCGCGT AGGACTGGTG CGGCTCGTCA GCCGCGCCAC ACAGTCGCCC 
AATCTACTCC AGGCGCAGAC GAATGCCCTG CCTGCTGCCT TCCAGCAGCG ATGCAGCATC 
TCCGGCAAGA CGATGCGAGG TGGACCACGG GTGCCCAAGG CCGCCCCCTA TCCGTACAAG 
ACGAAGAAGT ACAGCGTGTT CAATGCTATT TTCGACAAGA CCTCCAAGCG TTTTGACGAG 
AACTCCAAGG TGATCTGTGT GGAGGGACCC ATCGCGGCCG GCAAGTCAAA GTTCGCCAAG 
GAACTGGCTG AGGAGCTGGA CATGGAGTAC TATCCGGCAG TTGATCTGGA CCTAATCTAC 
ATCAATTCGT ACGGCTATGA CATGAGGAAG TTGGATCCTC AGTTGCCGCC CAGCTGCCGA 
AGCTACGATG TGCGCAACTT CTGCCTGGAT CCCAGCCACG ATCTGGCTGC CCAGTTCCAG 
ATTCGCATGT ACATGCTGCG CTATTCGCAG TACATTGATG CCCTGCAGCA CGTCCTTAGC 
ACCGGCCAGG GTGTTGTCCT CGAGCGCAGC CCCTACTCGG ACTTTGTCTT CATGGAGGCC 
ATGTTTCGGC AGGGTTATCT GTCACGCGGT GCGCGTTCCG TGTATAATGA GCTCCGACAG 
AACACCATCG GGGAGCTGCT GAAGCCGCAT TTGGTTATCT ACTTGGATCT GCCGGTCGAT 
GCCGTGAAGA AGCAGATCAA GGCACGCAAT GTGGACTACG AGGTGCAGTC AAAGGTGTTC 
AGCGATGCCT ACTTGAGCGA TTTGGAGCAG CTGTACAAGC AGCAGTACCT CAAGGACATC 
TCCACCCATG CCGAGCTGCT CATCTACGAC TGGACAGCCG GCGGTGAGAC TGAGGTTGTG 
GTGGAGGATA TCGAACGCAT CGACTTCAAC CAGTTTGAGG CCGATATTCA CAACAAGAAG 
ATGCTCGACT GGCGTTTCCC GCTGGAGGCC GAGTGGTGCG AAGCCCGTAT CAAGTACTGC 
CATGAGAAGC CCGATCTGAT GAATTACTTC AATGTGCCGC GTTTCGATGT CCCGGAGCTG 
GTGCGCAGCG CTGACGACGG CAAAGTCTGG CGTGATGTCT GGTTCAATGC TCCCGGCATG 
AAGTACCGTC CTGGCTACAA TGCAGACATG GGCGACGAGG GTCTCCTCAC CAAGACGAAA 
ATAGGCATCA ACCAGGGCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ND-42 ( NM_001275892.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262821.1 Drosophila melanogaster NADH dehydrogenase (ubiquinone) 42 kDa subunit (ND-42), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05650
Clone ID Related Accession (Same CDS sequence)	NM_169990.3 , NM_079721.4 , NM_001275892.1
Accession Version	NM_001275892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NADH dehydrogenase (ubiquinone) 42 kDa subunit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGCCG TGTTCCGCGT AGGACTGGTG CGGCTCGTCA GCCGCGCCAC ACAGTCGCCC 
AATCTACTCC AGGCGCAGAC GAATGCCCTG CCTGCTGCCT TCCAGCAGCG ATGCAGCATC 
TCCGGCAAGA CGATGCGAGG TGGACCACGG GTGCCCAAGG CCGCCCCCTA TCCGTACAAG 
ACGAAGAAGT ACAGCGTGTT CAATGCTATT TTCGACAAGA CCTCCAAGCG TTTTGACGAG 
AACTCCAAGG TGATCTGTGT GGAGGGACCC ATCGCGGCCG GCAAGTCAAA GTTCGCCAAG 
GAACTGGCTG AGGAGCTGGA CATGGAGTAC TATCCGGCAG TTGATCTGGA CCTAATCTAC 
ATCAATTCGT ACGGCTATGA CATGAGGAAG TTGGATCCTC AGTTGCCGCC CAGCTGCCGA 
AGCTACGATG TGCGCAACTT CTGCCTGGAT CCCAGCCACG ATCTGGCTGC CCAGTTCCAG 
ATTCGCATGT ACATGCTGCG CTATTCGCAG TACATTGATG CCCTGCAGCA CGTCCTTAGC 
ACCGGCCAGG GTGTTGTCCT CGAGCGCAGC CCCTACTCGG ACTTTGTCTT CATGGAGGCC 
ATGTTTCGGC AGGGTTATCT GTCACGCGGT GCGCGTTCCG TGTATAATGA GCTCCGACAG 
AACACCATCG GGGAGCTGCT GAAGCCGCAT TTGGTTATCT ACTTGGATCT GCCGGTCGAT 
GCCGTGAAGA AGCAGATCAA GGCACGCAAT GTGGACTACG AGGTGCAGTC AAAGGTGTTC 
AGCGATGCCT ACTTGAGCGA TTTGGAGCAG CTGTACAAGC AGCAGTACCT CAAGGACATC 
TCCACCCATG CCGAGCTGCT CATCTACGAC TGGACAGCCG GCGGTGAGAC TGAGGTTGTG 
GTGGAGGATA TCGAACGCAT CGACTTCAAC CAGTTTGAGG CCGATATTCA CAACAAGAAG 
ATGCTCGACT GGCGTTTCCC GCTGGAGGCC GAGTGGTGCG AAGCCCGTAT CAAGTACTGC 
CATGAGAAGC CCGATCTGAT GAATTACTTC AATGTGCCGC GTTTCGATGT CCCGGAGCTG 
GTGCGCAGCG CTGACGACGG CAAAGTCTGG CGTGATGTCT GGTTCAATGC TCCCGGCATG 
AAGTACCGTC CTGGCTACAA TGCAGACATG GGCGACGAGG GTCTCCTCAC CAAGACGAAA 
ATAGGCATCA ACCAGGGCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262821.1
CDS	118..1341
Translation	MTAVFRVGLVRLVSRATQSPNLLQAQTNALPAAFQQRCSISGKTMRGGPRVPKAAPYPYKTKKYSVFNAIFDKTSKRFDENSKVICVEGPIAAGKSKFAKELAEELDMEYYPAVDLDLIYINSYGYDMRKLDPQLPPSCRSYDVRNFCLDPSHDLAAQFQIRMYMLRYSQYIDALQHVLSTGQGVVLERSPYSDFVFMEAMFRQGYLSRGARSVYNELRQNTIGELLKPHLVIYLDLPVDAVKKQIKARNVDYEVQSKVFSDAYLSDLEQLYKQQYLKDISTHAELLIYDWTAGGETEVVVEDIERIDFNQFEADIHNKKMLDWRFPLEAEWCEARIKYCHEKPDLMNYFNVPRFDVPELVRSADDGKVWRDVWFNAPGMKYRPGYNADMGDEGLLTKTKIGINQGI

Target ORF information:

RefSeq Version	NM_001275892.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NADH dehydrogenase (ubiquinone) 42 kDa subunit (ND-42), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275892.1

ORF Insert Sequence:

ATGACCGCCG TGTTCCGCGT AGGACTGGTG CGGCTCGTCA GCCGCGCCAC ACAGTCGCCC 
AATCTACTCC AGGCGCAGAC GAATGCCCTG CCTGCTGCCT TCCAGCAGCG ATGCAGCATC 
TCCGGCAAGA CGATGCGAGG TGGACCACGG GTGCCCAAGG CCGCCCCCTA TCCGTACAAG 
ACGAAGAAGT ACAGCGTGTT CAATGCTATT TTCGACAAGA CCTCCAAGCG TTTTGACGAG 
AACTCCAAGG TGATCTGTGT GGAGGGACCC ATCGCGGCCG GCAAGTCAAA GTTCGCCAAG 
GAACTGGCTG AGGAGCTGGA CATGGAGTAC TATCCGGCAG TTGATCTGGA CCTAATCTAC 
ATCAATTCGT ACGGCTATGA CATGAGGAAG TTGGATCCTC AGTTGCCGCC CAGCTGCCGA 
AGCTACGATG TGCGCAACTT CTGCCTGGAT CCCAGCCACG ATCTGGCTGC CCAGTTCCAG 
ATTCGCATGT ACATGCTGCG CTATTCGCAG TACATTGATG CCCTGCAGCA CGTCCTTAGC 
ACCGGCCAGG GTGTTGTCCT CGAGCGCAGC CCCTACTCGG ACTTTGTCTT CATGGAGGCC 
ATGTTTCGGC AGGGTTATCT GTCACGCGGT GCGCGTTCCG TGTATAATGA GCTCCGACAG 
AACACCATCG GGGAGCTGCT GAAGCCGCAT TTGGTTATCT ACTTGGATCT GCCGGTCGAT 
GCCGTGAAGA AGCAGATCAA GGCACGCAAT GTGGACTACG AGGTGCAGTC AAAGGTGTTC 
AGCGATGCCT ACTTGAGCGA TTTGGAGCAG CTGTACAAGC AGCAGTACCT CAAGGACATC 
TCCACCCATG CCGAGCTGCT CATCTACGAC TGGACAGCCG GCGGTGAGAC TGAGGTTGTG 
GTGGAGGATA TCGAACGCAT CGACTTCAAC CAGTTTGAGG CCGATATTCA CAACAAGAAG 
ATGCTCGACT GGCGTTTCCC GCTGGAGGCC GAGTGGTGCG AAGCCCGTAT CAAGTACTGC 
CATGAGAAGC CCGATCTGAT GAATTACTTC AATGTGCCGC GTTTCGATGT CCCGGAGCTG 
GTGCGCAGCG CTGACGACGG CAAAGTCTGG CGTGATGTCT GGTTCAATGC TCCCGGCATG 
AAGTACCGTC CTGGCTACAA TGCAGACATG GGCGACGAGG GTCTCCTCAC CAAGACGAAA 
ATAGGCATCA ACCAGGGCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ND-42 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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