E2f1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following E2f1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E2f1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa05611 NM_079713.3
Latest version!
Drosophila melanogaster E2F transcription factor 1 (E2f1), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa05611 NM_169962.3
Latest version!
Drosophila melanogaster E2F transcription factor 1 (E2f1), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa05611 NM_001300526.1
Latest version!
Drosophila melanogaster E2F transcription factor 1 (E2f1), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa05611 NM_169961.3
Latest version!
Drosophila melanogaster E2F transcription factor 1 (E2f1), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa08624 NM_001275880.2
Latest version!
Drosophila melanogaster E2F transcription factor 1 (E2f1), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa08624 NM_001300525.1
Latest version!
Drosophila melanogaster E2F transcription factor 1 (E2f1), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa05611
    Clone ID Related Accession (Same CDS sequence) NM_169961.3 , NM_169962.3 , NM_079713.3 , NM_001300526.1
    Accession Version NM_169961.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product E2F transcription factor 1, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169961.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCAAGT TCTTTGTGAA TGTTGCCCCC ATCAACAACA GCAACAGCAG CAGTAGCCAC 
    ACAACCACCA GCAGCAACAC CCAGCGCCAC CAGCAGCACC AACAACACTA TGGCGGGAGC
    GGGACAACCG GCCACACGAT GGTGGCCCGC AGACTCAACT ACGATCTGCA CGGCGGCACC
    ACCAGCATCA ACAACAACAA CAACATTGTG ATCAAGAACG AGTCCGTCGA CTTGGACTAC
    GATCACGTCC TGAGCAGCAG CGACAGCAAC AGCAACGGCG GCGTGGCAGC CCACCTGCGG
    GATCATGTCT ACATCAGTCT GGACAAGGGG CACAACACGG GGGCAGTGGC AACAGCAGCG
    GCGGCGGCAA CGGCGGGCCA CACGCAGCAG CAACTGCAGC AGCAGCACCA CCACCAAAAT
    CAGCAGCAAC GCAAGGCGAC CGGCAAATCC AACGATATCA CAAATTATTA CAAGGTCAAA
    CGTCGGCCAC ACGCCGTCAG CGATGAGATT CACCCGAAGA AGCAGGCCAA GCAGAGCGCC
    CACCACCAGA CGGTGTATCA GAAGCATACG GCTAGCAGTG CGCCCCAGCA GCTCCGGCAC
    AGCCACCACC AGCTGCGCCA CGACGCGGAT GCCGAACTGG ACGAGGATGT GGTGGAGCGG
    GTGGCCAAGC CGGCATCGCA TCACCCCTTT TCGCTGTCGA CGCCCCAGCA ACAGTTGGCT
    GCGTCGGTGG CCAGCAGTTC CTCGAGCGGA GATCGCAATC GGGCGGACAC CTCGCTGGGA
    ATACTGACCA AAAAGTTTGT GGACCTGCTG CAGGAGTCAC CGGATGGCGT GGTGGATCTG
    AACGAGGCCT CCAATCGATT GCACGTTCAG AAGCGGCGCA TCTACGACAT TACCAATGTC
    CTCGAAGGCA TTAACATCCT GGAGAAGAAG TCGAAGAACA ACATCCAGTG GCGCTGCGGG
    CAGTCGATGG TCAGCCAGGA GCGTTCCCGT CACATCGAAG CGGATTCGTT GCGGCTGGAG
    CAACAGGAGA ACGAGCTGAA CAAGGCCATC GATCTGATGC GCGAGAATCT GGCTGAGATC
    TCCCAGGAGG TGGAGAACTC CGGCGGCATG GCCTACGTCA CGCAGAACGA CCTGCTCAAC
    GTGGATCTCT TCAAAGATCA GATCGTTATC GTGATCAAGG CGCCGCCAGA GGCTAAGCTT
    GTGCTGCCCA ACACAAAATT GCCGCGCGAG ATTTACGTGA AGGCGGAGAA TAGCGGGGAG
    ATCAACGTCT TCCTCTGCCA TGACACCTCA CCGGAAAATT CGCCAATCGC GCCGGGCGCC
    GGCTACGTTG GAGCACCCGG TGCAGGATGC GTCCGTACGG CCACCTCCAC ACGACTGCAT
    CCGTTGACCA ACCAGCGACT GAACGATCCG CTCTTCAACA ATATCGATGC CATGAGCACC
    AAGGGATTAT TTCAAACGCC CTACCGCTCG GCTCGCAACC TCAGCAAATC GATTGAGGAA
    GCCGCCAAGC AGTCCCAGCC TGAATATAAT AACATTTGTG ATATTGCGAT GGGCCAGCAT
    CATAATCTGA ACCAGCAGCA GCAGCAGCAA CAACAACAGT TGCTACAGCA GCCGGAGGAG
    GACGATGTGG ACGTGGAGCT GAACCAGCTG GTTCCGACAC TGACGAATCC TGTGGTCCGG
    ACCCATCAGT TCCAGCAGCA CCAGCAGCCC TCCATCCAGG AGCTGTTCAG CAGCTTAACA
    GAATCCTCGC CTCCAACGCC CACCAAGCGG CGTCGGGAGG CAGCAGCCGC AGCAATTGCC
    GCAGGCAGCT CAACAACAGC AACCACTACG CTTAATAGTC ATAACAACAG AAACCACAGC
    AATCATAGCA ACCACAGCAA CCATAGCAGC AGCAACAACA GCAAATCCCA ACCACCCACA
    ATAGGCTACG GCAGCAGTCA GCGACGCAGC GATGTGCCCA TGTATAATTG TGCAATGGAA
    GGCGCAACCA CAACATCTGC AACAGCAGAT ACGACAGCTG CCACATCGCG ATCGGCCGCA
    GCAAGCTCAC TGCAGATGCA ATTCGCTGCC GTCGCGGAGA GCAACAACGG CAGTAGCAGC
    GGCGGTGGTG GCGGCGGCGG CGGCTATGGC AGCATCGCTG GCGCTGGCGC CAACGCTGAT
    CCCCATCAGC CGTACAGCCA TGACCGCAAC AGCCTGCCGC CCGGCGTGGC TGACTGCGAT
    GCCAACAGCA ACAGCAGCAG TGTCACGCTG CAGGGCCTCG ATGCTCTCTT CAACGACATT
    GGCTCGGACT ACTTCAGCAA CGATATCGCC TTTGTGTCCA TCAATCCGCC GGACGACAAT
    GACTATCCGT ATGCGCTGAA CGCGAACGAG GGCATCGATC GGCTGTTTGA CTTCGGTTCG
    GATGCCTATG GACCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_732646.1
    CDS532..2949
    Translation

    Target ORF information:

    RefSeq Version NM_169961.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster E2F transcription factor 1 (E2f1), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169961.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCAAGT TCTTTGTGAA TGTTGCCCCC ATCAACAACA GCAACAGCAG CAGTAGCCAC 
    ACAACCACCA GCAGCAACAC CCAGCGCCAC CAGCAGCACC AACAACACTA TGGCGGGAGC
    GGGACAACCG GCCACACGAT GGTGGCCCGC AGACTCAACT ACGATCTGCA CGGCGGCACC
    ACCAGCATCA ACAACAACAA CAACATTGTG ATCAAGAACG AGTCCGTCGA CTTGGACTAC
    GATCACGTCC TGAGCAGCAG CGACAGCAAC AGCAACGGCG GCGTGGCAGC CCACCTGCGG
    GATCATGTCT ACATCAGTCT GGACAAGGGG CACAACACGG GGGCAGTGGC AACAGCAGCG
    GCGGCGGCAA CGGCGGGCCA CACGCAGCAG CAACTGCAGC AGCAGCACCA CCACCAAAAT
    CAGCAGCAAC GCAAGGCGAC CGGCAAATCC AACGATATCA CAAATTATTA CAAGGTCAAA
    CGTCGGCCAC ACGCCGTCAG CGATGAGATT CACCCGAAGA AGCAGGCCAA GCAGAGCGCC
    CACCACCAGA CGGTGTATCA GAAGCATACG GCTAGCAGTG CGCCCCAGCA GCTCCGGCAC
    AGCCACCACC AGCTGCGCCA CGACGCGGAT GCCGAACTGG ACGAGGATGT GGTGGAGCGG
    GTGGCCAAGC CGGCATCGCA TCACCCCTTT TCGCTGTCGA CGCCCCAGCA ACAGTTGGCT
    GCGTCGGTGG CCAGCAGTTC CTCGAGCGGA GATCGCAATC GGGCGGACAC CTCGCTGGGA
    ATACTGACCA AAAAGTTTGT GGACCTGCTG CAGGAGTCAC CGGATGGCGT GGTGGATCTG
    AACGAGGCCT CCAATCGATT GCACGTTCAG AAGCGGCGCA TCTACGACAT TACCAATGTC
    CTCGAAGGCA TTAACATCCT GGAGAAGAAG TCGAAGAACA ACATCCAGTG GCGCTGCGGG
    CAGTCGATGG TCAGCCAGGA GCGTTCCCGT CACATCGAAG CGGATTCGTT GCGGCTGGAG
    CAACAGGAGA ACGAGCTGAA CAAGGCCATC GATCTGATGC GCGAGAATCT GGCTGAGATC
    TCCCAGGAGG TGGAGAACTC CGGCGGCATG GCCTACGTCA CGCAGAACGA CCTGCTCAAC
    GTGGATCTCT TCAAAGATCA GATCGTTATC GTGATCAAGG CGCCGCCAGA GGCTAAGCTT
    GTGCTGCCCA ACACAAAATT GCCGCGCGAG ATTTACGTGA AGGCGGAGAA TAGCGGGGAG
    ATCAACGTCT TCCTCTGCCA TGACACCTCA CCGGAAAATT CGCCAATCGC GCCGGGCGCC
    GGCTACGTTG GAGCACCCGG TGCAGGATGC GTCCGTACGG CCACCTCCAC ACGACTGCAT
    CCGTTGACCA ACCAGCGACT GAACGATCCG CTCTTCAACA ATATCGATGC CATGAGCACC
    AAGGGATTAT TTCAAACGCC CTACCGCTCG GCTCGCAACC TCAGCAAATC GATTGAGGAA
    GCCGCCAAGC AGTCCCAGCC TGAATATAAT AACATTTGTG ATATTGCGAT GGGCCAGCAT
    CATAATCTGA ACCAGCAGCA GCAGCAGCAA CAACAACAGT TGCTACAGCA GCCGGAGGAG
    GACGATGTGG ACGTGGAGCT GAACCAGCTG GTTCCGACAC TGACGAATCC TGTGGTCCGG
    ACCCATCAGT TCCAGCAGCA CCAGCAGCCC TCCATCCAGG AGCTGTTCAG CAGCTTAACA
    GAATCCTCGC CTCCAACGCC CACCAAGCGG CGTCGGGAGG CAGCAGCCGC AGCAATTGCC
    GCAGGCAGCT CAACAACAGC AACCACTACG CTTAATAGTC ATAACAACAG AAACCACAGC
    AATCATAGCA ACCACAGCAA CCATAGCAGC AGCAACAACA GCAAATCCCA ACCACCCACA
    ATAGGCTACG GCAGCAGTCA GCGACGCAGC GATGTGCCCA TGTATAATTG TGCAATGGAA
    GGCGCAACCA CAACATCTGC AACAGCAGAT ACGACAGCTG CCACATCGCG ATCGGCCGCA
    GCAAGCTCAC TGCAGATGCA ATTCGCTGCC GTCGCGGAGA GCAACAACGG CAGTAGCAGC
    GGCGGTGGTG GCGGCGGCGG CGGCTATGGC AGCATCGCTG GCGCTGGCGC CAACGCTGAT
    CCCCATCAGC CGTACAGCCA TGACCGCAAC AGCCTGCCGC CCGGCGTGGC TGACTGCGAT
    GCCAACAGCA ACAGCAGCAG TGTCACGCTG CAGGGCCTCG ATGCTCTCTT CAACGACATT
    GGCTCGGACT ACTTCAGCAA CGATATCGCC TTTGTGTCCA TCAATCCGCC GGACGACAAT
    GACTATCCGT ATGCGCTGAA CGCGAACGAG GGCATCGATC GGCTGTTTGA CTTCGGTTCG
    GATGCCTATG GACCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05611
    Clone ID Related Accession (Same CDS sequence) NM_169961.3 , NM_169962.3 , NM_079713.3 , NM_001300526.1
    Accession Version NM_169962.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product E2F transcription factor 1, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_169962.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCAAGT TCTTTGTGAA TGTTGCCCCC ATCAACAACA GCAACAGCAG CAGTAGCCAC 
    ACAACCACCA GCAGCAACAC CCAGCGCCAC CAGCAGCACC AACAACACTA TGGCGGGAGC
    GGGACAACCG GCCACACGAT GGTGGCCCGC AGACTCAACT ACGATCTGCA CGGCGGCACC
    ACCAGCATCA ACAACAACAA CAACATTGTG ATCAAGAACG AGTCCGTCGA CTTGGACTAC
    GATCACGTCC TGAGCAGCAG CGACAGCAAC AGCAACGGCG GCGTGGCAGC CCACCTGCGG
    GATCATGTCT ACATCAGTCT GGACAAGGGG CACAACACGG GGGCAGTGGC AACAGCAGCG
    GCGGCGGCAA CGGCGGGCCA CACGCAGCAG CAACTGCAGC AGCAGCACCA CCACCAAAAT
    CAGCAGCAAC GCAAGGCGAC CGGCAAATCC AACGATATCA CAAATTATTA CAAGGTCAAA
    CGTCGGCCAC ACGCCGTCAG CGATGAGATT CACCCGAAGA AGCAGGCCAA GCAGAGCGCC
    CACCACCAGA CGGTGTATCA GAAGCATACG GCTAGCAGTG CGCCCCAGCA GCTCCGGCAC
    AGCCACCACC AGCTGCGCCA CGACGCGGAT GCCGAACTGG ACGAGGATGT GGTGGAGCGG
    GTGGCCAAGC CGGCATCGCA TCACCCCTTT TCGCTGTCGA CGCCCCAGCA ACAGTTGGCT
    GCGTCGGTGG CCAGCAGTTC CTCGAGCGGA GATCGCAATC GGGCGGACAC CTCGCTGGGA
    ATACTGACCA AAAAGTTTGT GGACCTGCTG CAGGAGTCAC CGGATGGCGT GGTGGATCTG
    AACGAGGCCT CCAATCGATT GCACGTTCAG AAGCGGCGCA TCTACGACAT TACCAATGTC
    CTCGAAGGCA TTAACATCCT GGAGAAGAAG TCGAAGAACA ACATCCAGTG GCGCTGCGGG
    CAGTCGATGG TCAGCCAGGA GCGTTCCCGT CACATCGAAG CGGATTCGTT GCGGCTGGAG
    CAACAGGAGA ACGAGCTGAA CAAGGCCATC GATCTGATGC GCGAGAATCT GGCTGAGATC
    TCCCAGGAGG TGGAGAACTC CGGCGGCATG GCCTACGTCA CGCAGAACGA CCTGCTCAAC
    GTGGATCTCT TCAAAGATCA GATCGTTATC GTGATCAAGG CGCCGCCAGA GGCTAAGCTT
    GTGCTGCCCA ACACAAAATT GCCGCGCGAG ATTTACGTGA AGGCGGAGAA TAGCGGGGAG
    ATCAACGTCT TCCTCTGCCA TGACACCTCA CCGGAAAATT CGCCAATCGC GCCGGGCGCC
    GGCTACGTTG GAGCACCCGG TGCAGGATGC GTCCGTACGG CCACCTCCAC ACGACTGCAT
    CCGTTGACCA ACCAGCGACT GAACGATCCG CTCTTCAACA ATATCGATGC CATGAGCACC
    AAGGGATTAT TTCAAACGCC CTACCGCTCG GCTCGCAACC TCAGCAAATC GATTGAGGAA
    GCCGCCAAGC AGTCCCAGCC TGAATATAAT AACATTTGTG ATATTGCGAT GGGCCAGCAT
    CATAATCTGA ACCAGCAGCA GCAGCAGCAA CAACAACAGT TGCTACAGCA GCCGGAGGAG
    GACGATGTGG ACGTGGAGCT GAACCAGCTG GTTCCGACAC TGACGAATCC TGTGGTCCGG
    ACCCATCAGT TCCAGCAGCA CCAGCAGCCC TCCATCCAGG AGCTGTTCAG CAGCTTAACA
    GAATCCTCGC CTCCAACGCC CACCAAGCGG CGTCGGGAGG CAGCAGCCGC AGCAATTGCC
    GCAGGCAGCT CAACAACAGC AACCACTACG CTTAATAGTC ATAACAACAG AAACCACAGC
    AATCATAGCA ACCACAGCAA CCATAGCAGC AGCAACAACA GCAAATCCCA ACCACCCACA
    ATAGGCTACG GCAGCAGTCA GCGACGCAGC GATGTGCCCA TGTATAATTG TGCAATGGAA
    GGCGCAACCA CAACATCTGC AACAGCAGAT ACGACAGCTG CCACATCGCG ATCGGCCGCA
    GCAAGCTCAC TGCAGATGCA ATTCGCTGCC GTCGCGGAGA GCAACAACGG CAGTAGCAGC
    GGCGGTGGTG GCGGCGGCGG CGGCTATGGC AGCATCGCTG GCGCTGGCGC CAACGCTGAT
    CCCCATCAGC CGTACAGCCA TGACCGCAAC AGCCTGCCGC CCGGCGTGGC TGACTGCGAT
    GCCAACAGCA ACAGCAGCAG TGTCACGCTG CAGGGCCTCG ATGCTCTCTT CAACGACATT
    GGCTCGGACT ACTTCAGCAA CGATATCGCC TTTGTGTCCA TCAATCCGCC GGACGACAAT
    GACTATCCGT ATGCGCTGAA CGCGAACGAG GGCATCGATC GGCTGTTTGA CTTCGGTTCG
    GATGCCTATG GACCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_732647.1
    CDS1096..3513
    Translation

    Target ORF information:

    RefSeq Version NM_169962.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster E2F transcription factor 1 (E2f1), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169962.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCAAGT TCTTTGTGAA TGTTGCCCCC ATCAACAACA GCAACAGCAG CAGTAGCCAC 
    ACAACCACCA GCAGCAACAC CCAGCGCCAC CAGCAGCACC AACAACACTA TGGCGGGAGC
    GGGACAACCG GCCACACGAT GGTGGCCCGC AGACTCAACT ACGATCTGCA CGGCGGCACC
    ACCAGCATCA ACAACAACAA CAACATTGTG ATCAAGAACG AGTCCGTCGA CTTGGACTAC
    GATCACGTCC TGAGCAGCAG CGACAGCAAC AGCAACGGCG GCGTGGCAGC CCACCTGCGG
    GATCATGTCT ACATCAGTCT GGACAAGGGG CACAACACGG GGGCAGTGGC AACAGCAGCG
    GCGGCGGCAA CGGCGGGCCA CACGCAGCAG CAACTGCAGC AGCAGCACCA CCACCAAAAT
    CAGCAGCAAC GCAAGGCGAC CGGCAAATCC AACGATATCA CAAATTATTA CAAGGTCAAA
    CGTCGGCCAC ACGCCGTCAG CGATGAGATT CACCCGAAGA AGCAGGCCAA GCAGAGCGCC
    CACCACCAGA CGGTGTATCA GAAGCATACG GCTAGCAGTG CGCCCCAGCA GCTCCGGCAC
    AGCCACCACC AGCTGCGCCA CGACGCGGAT GCCGAACTGG ACGAGGATGT GGTGGAGCGG
    GTGGCCAAGC CGGCATCGCA TCACCCCTTT TCGCTGTCGA CGCCCCAGCA ACAGTTGGCT
    GCGTCGGTGG CCAGCAGTTC CTCGAGCGGA GATCGCAATC GGGCGGACAC CTCGCTGGGA
    ATACTGACCA AAAAGTTTGT GGACCTGCTG CAGGAGTCAC CGGATGGCGT GGTGGATCTG
    AACGAGGCCT CCAATCGATT GCACGTTCAG AAGCGGCGCA TCTACGACAT TACCAATGTC
    CTCGAAGGCA TTAACATCCT GGAGAAGAAG TCGAAGAACA ACATCCAGTG GCGCTGCGGG
    CAGTCGATGG TCAGCCAGGA GCGTTCCCGT CACATCGAAG CGGATTCGTT GCGGCTGGAG
    CAACAGGAGA ACGAGCTGAA CAAGGCCATC GATCTGATGC GCGAGAATCT GGCTGAGATC
    TCCCAGGAGG TGGAGAACTC CGGCGGCATG GCCTACGTCA CGCAGAACGA CCTGCTCAAC
    GTGGATCTCT TCAAAGATCA GATCGTTATC GTGATCAAGG CGCCGCCAGA GGCTAAGCTT
    GTGCTGCCCA ACACAAAATT GCCGCGCGAG ATTTACGTGA AGGCGGAGAA TAGCGGGGAG
    ATCAACGTCT TCCTCTGCCA TGACACCTCA CCGGAAAATT CGCCAATCGC GCCGGGCGCC
    GGCTACGTTG GAGCACCCGG TGCAGGATGC GTCCGTACGG CCACCTCCAC ACGACTGCAT
    CCGTTGACCA ACCAGCGACT GAACGATCCG CTCTTCAACA ATATCGATGC CATGAGCACC
    AAGGGATTAT TTCAAACGCC CTACCGCTCG GCTCGCAACC TCAGCAAATC GATTGAGGAA
    GCCGCCAAGC AGTCCCAGCC TGAATATAAT AACATTTGTG ATATTGCGAT GGGCCAGCAT
    CATAATCTGA ACCAGCAGCA GCAGCAGCAA CAACAACAGT TGCTACAGCA GCCGGAGGAG
    GACGATGTGG ACGTGGAGCT GAACCAGCTG GTTCCGACAC TGACGAATCC TGTGGTCCGG
    ACCCATCAGT TCCAGCAGCA CCAGCAGCCC TCCATCCAGG AGCTGTTCAG CAGCTTAACA
    GAATCCTCGC CTCCAACGCC CACCAAGCGG CGTCGGGAGG CAGCAGCCGC AGCAATTGCC
    GCAGGCAGCT CAACAACAGC AACCACTACG CTTAATAGTC ATAACAACAG AAACCACAGC
    AATCATAGCA ACCACAGCAA CCATAGCAGC AGCAACAACA GCAAATCCCA ACCACCCACA
    ATAGGCTACG GCAGCAGTCA GCGACGCAGC GATGTGCCCA TGTATAATTG TGCAATGGAA
    GGCGCAACCA CAACATCTGC AACAGCAGAT ACGACAGCTG CCACATCGCG ATCGGCCGCA
    GCAAGCTCAC TGCAGATGCA ATTCGCTGCC GTCGCGGAGA GCAACAACGG CAGTAGCAGC
    GGCGGTGGTG GCGGCGGCGG CGGCTATGGC AGCATCGCTG GCGCTGGCGC CAACGCTGAT
    CCCCATCAGC CGTACAGCCA TGACCGCAAC AGCCTGCCGC CCGGCGTGGC TGACTGCGAT
    GCCAACAGCA ACAGCAGCAG TGTCACGCTG CAGGGCCTCG ATGCTCTCTT CAACGACATT
    GGCTCGGACT ACTTCAGCAA CGATATCGCC TTTGTGTCCA TCAATCCGCC GGACGACAAT
    GACTATCCGT ATGCGCTGAA CGCGAACGAG GGCATCGATC GGCTGTTTGA CTTCGGTTCG
    GATGCCTATG GACCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05611
    Clone ID Related Accession (Same CDS sequence) NM_169961.3 , NM_169962.3 , NM_079713.3 , NM_001300526.1
    Accession Version NM_079713.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product E2F transcription factor 1, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079713.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCAAGT TCTTTGTGAA TGTTGCCCCC ATCAACAACA GCAACAGCAG CAGTAGCCAC 
    ACAACCACCA GCAGCAACAC CCAGCGCCAC CAGCAGCACC AACAACACTA TGGCGGGAGC
    GGGACAACCG GCCACACGAT GGTGGCCCGC AGACTCAACT ACGATCTGCA CGGCGGCACC
    ACCAGCATCA ACAACAACAA CAACATTGTG ATCAAGAACG AGTCCGTCGA CTTGGACTAC
    GATCACGTCC TGAGCAGCAG CGACAGCAAC AGCAACGGCG GCGTGGCAGC CCACCTGCGG
    GATCATGTCT ACATCAGTCT GGACAAGGGG CACAACACGG GGGCAGTGGC AACAGCAGCG
    GCGGCGGCAA CGGCGGGCCA CACGCAGCAG CAACTGCAGC AGCAGCACCA CCACCAAAAT
    CAGCAGCAAC GCAAGGCGAC CGGCAAATCC AACGATATCA CAAATTATTA CAAGGTCAAA
    CGTCGGCCAC ACGCCGTCAG CGATGAGATT CACCCGAAGA AGCAGGCCAA GCAGAGCGCC
    CACCACCAGA CGGTGTATCA GAAGCATACG GCTAGCAGTG CGCCCCAGCA GCTCCGGCAC
    AGCCACCACC AGCTGCGCCA CGACGCGGAT GCCGAACTGG ACGAGGATGT GGTGGAGCGG
    GTGGCCAAGC CGGCATCGCA TCACCCCTTT TCGCTGTCGA CGCCCCAGCA ACAGTTGGCT
    GCGTCGGTGG CCAGCAGTTC CTCGAGCGGA GATCGCAATC GGGCGGACAC CTCGCTGGGA
    ATACTGACCA AAAAGTTTGT GGACCTGCTG CAGGAGTCAC CGGATGGCGT GGTGGATCTG
    AACGAGGCCT CCAATCGATT GCACGTTCAG AAGCGGCGCA TCTACGACAT TACCAATGTC
    CTCGAAGGCA TTAACATCCT GGAGAAGAAG TCGAAGAACA ACATCCAGTG GCGCTGCGGG
    CAGTCGATGG TCAGCCAGGA GCGTTCCCGT CACATCGAAG CGGATTCGTT GCGGCTGGAG
    CAACAGGAGA ACGAGCTGAA CAAGGCCATC GATCTGATGC GCGAGAATCT GGCTGAGATC
    TCCCAGGAGG TGGAGAACTC CGGCGGCATG GCCTACGTCA CGCAGAACGA CCTGCTCAAC
    GTGGATCTCT TCAAAGATCA GATCGTTATC GTGATCAAGG CGCCGCCAGA GGCTAAGCTT
    GTGCTGCCCA ACACAAAATT GCCGCGCGAG ATTTACGTGA AGGCGGAGAA TAGCGGGGAG
    ATCAACGTCT TCCTCTGCCA TGACACCTCA CCGGAAAATT CGCCAATCGC GCCGGGCGCC
    GGCTACGTTG GAGCACCCGG TGCAGGATGC GTCCGTACGG CCACCTCCAC ACGACTGCAT
    CCGTTGACCA ACCAGCGACT GAACGATCCG CTCTTCAACA ATATCGATGC CATGAGCACC
    AAGGGATTAT TTCAAACGCC CTACCGCTCG GCTCGCAACC TCAGCAAATC GATTGAGGAA
    GCCGCCAAGC AGTCCCAGCC TGAATATAAT AACATTTGTG ATATTGCGAT GGGCCAGCAT
    CATAATCTGA ACCAGCAGCA GCAGCAGCAA CAACAACAGT TGCTACAGCA GCCGGAGGAG
    GACGATGTGG ACGTGGAGCT GAACCAGCTG GTTCCGACAC TGACGAATCC TGTGGTCCGG
    ACCCATCAGT TCCAGCAGCA CCAGCAGCCC TCCATCCAGG AGCTGTTCAG CAGCTTAACA
    GAATCCTCGC CTCCAACGCC CACCAAGCGG CGTCGGGAGG CAGCAGCCGC AGCAATTGCC
    GCAGGCAGCT CAACAACAGC AACCACTACG CTTAATAGTC ATAACAACAG AAACCACAGC
    AATCATAGCA ACCACAGCAA CCATAGCAGC AGCAACAACA GCAAATCCCA ACCACCCACA
    ATAGGCTACG GCAGCAGTCA GCGACGCAGC GATGTGCCCA TGTATAATTG TGCAATGGAA
    GGCGCAACCA CAACATCTGC AACAGCAGAT ACGACAGCTG CCACATCGCG ATCGGCCGCA
    GCAAGCTCAC TGCAGATGCA ATTCGCTGCC GTCGCGGAGA GCAACAACGG CAGTAGCAGC
    GGCGGTGGTG GCGGCGGCGG CGGCTATGGC AGCATCGCTG GCGCTGGCGC CAACGCTGAT
    CCCCATCAGC CGTACAGCCA TGACCGCAAC AGCCTGCCGC CCGGCGTGGC TGACTGCGAT
    GCCAACAGCA ACAGCAGCAG TGTCACGCTG CAGGGCCTCG ATGCTCTCTT CAACGACATT
    GGCTCGGACT ACTTCAGCAA CGATATCGCC TTTGTGTCCA TCAATCCGCC GGACGACAAT
    GACTATCCGT ATGCGCTGAA CGCGAACGAG GGCATCGATC GGCTGTTTGA CTTCGGTTCG
    GATGCCTATG GACCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_524437.2
    CDS1295..3712
    Translation

    Target ORF information:

    RefSeq Version NM_079713.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster E2F transcription factor 1 (E2f1), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_079713.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCAAGT TCTTTGTGAA TGTTGCCCCC ATCAACAACA GCAACAGCAG CAGTAGCCAC 
    ACAACCACCA GCAGCAACAC CCAGCGCCAC CAGCAGCACC AACAACACTA TGGCGGGAGC
    GGGACAACCG GCCACACGAT GGTGGCCCGC AGACTCAACT ACGATCTGCA CGGCGGCACC
    ACCAGCATCA ACAACAACAA CAACATTGTG ATCAAGAACG AGTCCGTCGA CTTGGACTAC
    GATCACGTCC TGAGCAGCAG CGACAGCAAC AGCAACGGCG GCGTGGCAGC CCACCTGCGG
    GATCATGTCT ACATCAGTCT GGACAAGGGG CACAACACGG GGGCAGTGGC AACAGCAGCG
    GCGGCGGCAA CGGCGGGCCA CACGCAGCAG CAACTGCAGC AGCAGCACCA CCACCAAAAT
    CAGCAGCAAC GCAAGGCGAC CGGCAAATCC AACGATATCA CAAATTATTA CAAGGTCAAA
    CGTCGGCCAC ACGCCGTCAG CGATGAGATT CACCCGAAGA AGCAGGCCAA GCAGAGCGCC
    CACCACCAGA CGGTGTATCA GAAGCATACG GCTAGCAGTG CGCCCCAGCA GCTCCGGCAC
    AGCCACCACC AGCTGCGCCA CGACGCGGAT GCCGAACTGG ACGAGGATGT GGTGGAGCGG
    GTGGCCAAGC CGGCATCGCA TCACCCCTTT TCGCTGTCGA CGCCCCAGCA ACAGTTGGCT
    GCGTCGGTGG CCAGCAGTTC CTCGAGCGGA GATCGCAATC GGGCGGACAC CTCGCTGGGA
    ATACTGACCA AAAAGTTTGT GGACCTGCTG CAGGAGTCAC CGGATGGCGT GGTGGATCTG
    AACGAGGCCT CCAATCGATT GCACGTTCAG AAGCGGCGCA TCTACGACAT TACCAATGTC
    CTCGAAGGCA TTAACATCCT GGAGAAGAAG TCGAAGAACA ACATCCAGTG GCGCTGCGGG
    CAGTCGATGG TCAGCCAGGA GCGTTCCCGT CACATCGAAG CGGATTCGTT GCGGCTGGAG
    CAACAGGAGA ACGAGCTGAA CAAGGCCATC GATCTGATGC GCGAGAATCT GGCTGAGATC
    TCCCAGGAGG TGGAGAACTC CGGCGGCATG GCCTACGTCA CGCAGAACGA CCTGCTCAAC
    GTGGATCTCT TCAAAGATCA GATCGTTATC GTGATCAAGG CGCCGCCAGA GGCTAAGCTT
    GTGCTGCCCA ACACAAAATT GCCGCGCGAG ATTTACGTGA AGGCGGAGAA TAGCGGGGAG
    ATCAACGTCT TCCTCTGCCA TGACACCTCA CCGGAAAATT CGCCAATCGC GCCGGGCGCC
    GGCTACGTTG GAGCACCCGG TGCAGGATGC GTCCGTACGG CCACCTCCAC ACGACTGCAT
    CCGTTGACCA ACCAGCGACT GAACGATCCG CTCTTCAACA ATATCGATGC CATGAGCACC
    AAGGGATTAT TTCAAACGCC CTACCGCTCG GCTCGCAACC TCAGCAAATC GATTGAGGAA
    GCCGCCAAGC AGTCCCAGCC TGAATATAAT AACATTTGTG ATATTGCGAT GGGCCAGCAT
    CATAATCTGA ACCAGCAGCA GCAGCAGCAA CAACAACAGT TGCTACAGCA GCCGGAGGAG
    GACGATGTGG ACGTGGAGCT GAACCAGCTG GTTCCGACAC TGACGAATCC TGTGGTCCGG
    ACCCATCAGT TCCAGCAGCA CCAGCAGCCC TCCATCCAGG AGCTGTTCAG CAGCTTAACA
    GAATCCTCGC CTCCAACGCC CACCAAGCGG CGTCGGGAGG CAGCAGCCGC AGCAATTGCC
    GCAGGCAGCT CAACAACAGC AACCACTACG CTTAATAGTC ATAACAACAG AAACCACAGC
    AATCATAGCA ACCACAGCAA CCATAGCAGC AGCAACAACA GCAAATCCCA ACCACCCACA
    ATAGGCTACG GCAGCAGTCA GCGACGCAGC GATGTGCCCA TGTATAATTG TGCAATGGAA
    GGCGCAACCA CAACATCTGC AACAGCAGAT ACGACAGCTG CCACATCGCG ATCGGCCGCA
    GCAAGCTCAC TGCAGATGCA ATTCGCTGCC GTCGCGGAGA GCAACAACGG CAGTAGCAGC
    GGCGGTGGTG GCGGCGGCGG CGGCTATGGC AGCATCGCTG GCGCTGGCGC CAACGCTGAT
    CCCCATCAGC CGTACAGCCA TGACCGCAAC AGCCTGCCGC CCGGCGTGGC TGACTGCGAT
    GCCAACAGCA ACAGCAGCAG TGTCACGCTG CAGGGCCTCG ATGCTCTCTT CAACGACATT
    GGCTCGGACT ACTTCAGCAA CGATATCGCC TTTGTGTCCA TCAATCCGCC GGACGACAAT
    GACTATCCGT ATGCGCTGAA CGCGAACGAG GGCATCGATC GGCTGTTTGA CTTCGGTTCG
    GATGCCTATG GACCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05611
    Clone ID Related Accession (Same CDS sequence) NM_169961.3 , NM_169962.3 , NM_079713.3 , NM_001300526.1
    Accession Version NM_001300526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product E2F transcription factor 1, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCAAGT TCTTTGTGAA TGTTGCCCCC ATCAACAACA GCAACAGCAG CAGTAGCCAC 
    ACAACCACCA GCAGCAACAC CCAGCGCCAC CAGCAGCACC AACAACACTA TGGCGGGAGC
    GGGACAACCG GCCACACGAT GGTGGCCCGC AGACTCAACT ACGATCTGCA CGGCGGCACC
    ACCAGCATCA ACAACAACAA CAACATTGTG ATCAAGAACG AGTCCGTCGA CTTGGACTAC
    GATCACGTCC TGAGCAGCAG CGACAGCAAC AGCAACGGCG GCGTGGCAGC CCACCTGCGG
    GATCATGTCT ACATCAGTCT GGACAAGGGG CACAACACGG GGGCAGTGGC AACAGCAGCG
    GCGGCGGCAA CGGCGGGCCA CACGCAGCAG CAACTGCAGC AGCAGCACCA CCACCAAAAT
    CAGCAGCAAC GCAAGGCGAC CGGCAAATCC AACGATATCA CAAATTATTA CAAGGTCAAA
    CGTCGGCCAC ACGCCGTCAG CGATGAGATT CACCCGAAGA AGCAGGCCAA GCAGAGCGCC
    CACCACCAGA CGGTGTATCA GAAGCATACG GCTAGCAGTG CGCCCCAGCA GCTCCGGCAC
    AGCCACCACC AGCTGCGCCA CGACGCGGAT GCCGAACTGG ACGAGGATGT GGTGGAGCGG
    GTGGCCAAGC CGGCATCGCA TCACCCCTTT TCGCTGTCGA CGCCCCAGCA ACAGTTGGCT
    GCGTCGGTGG CCAGCAGTTC CTCGAGCGGA GATCGCAATC GGGCGGACAC CTCGCTGGGA
    ATACTGACCA AAAAGTTTGT GGACCTGCTG CAGGAGTCAC CGGATGGCGT GGTGGATCTG
    AACGAGGCCT CCAATCGATT GCACGTTCAG AAGCGGCGCA TCTACGACAT TACCAATGTC
    CTCGAAGGCA TTAACATCCT GGAGAAGAAG TCGAAGAACA ACATCCAGTG GCGCTGCGGG
    CAGTCGATGG TCAGCCAGGA GCGTTCCCGT CACATCGAAG CGGATTCGTT GCGGCTGGAG
    CAACAGGAGA ACGAGCTGAA CAAGGCCATC GATCTGATGC GCGAGAATCT GGCTGAGATC
    TCCCAGGAGG TGGAGAACTC CGGCGGCATG GCCTACGTCA CGCAGAACGA CCTGCTCAAC
    GTGGATCTCT TCAAAGATCA GATCGTTATC GTGATCAAGG CGCCGCCAGA GGCTAAGCTT
    GTGCTGCCCA ACACAAAATT GCCGCGCGAG ATTTACGTGA AGGCGGAGAA TAGCGGGGAG
    ATCAACGTCT TCCTCTGCCA TGACACCTCA CCGGAAAATT CGCCAATCGC GCCGGGCGCC
    GGCTACGTTG GAGCACCCGG TGCAGGATGC GTCCGTACGG CCACCTCCAC ACGACTGCAT
    CCGTTGACCA ACCAGCGACT GAACGATCCG CTCTTCAACA ATATCGATGC CATGAGCACC
    AAGGGATTAT TTCAAACGCC CTACCGCTCG GCTCGCAACC TCAGCAAATC GATTGAGGAA
    GCCGCCAAGC AGTCCCAGCC TGAATATAAT AACATTTGTG ATATTGCGAT GGGCCAGCAT
    CATAATCTGA ACCAGCAGCA GCAGCAGCAA CAACAACAGT TGCTACAGCA GCCGGAGGAG
    GACGATGTGG ACGTGGAGCT GAACCAGCTG GTTCCGACAC TGACGAATCC TGTGGTCCGG
    ACCCATCAGT TCCAGCAGCA CCAGCAGCCC TCCATCCAGG AGCTGTTCAG CAGCTTAACA
    GAATCCTCGC CTCCAACGCC CACCAAGCGG CGTCGGGAGG CAGCAGCCGC AGCAATTGCC
    GCAGGCAGCT CAACAACAGC AACCACTACG CTTAATAGTC ATAACAACAG AAACCACAGC
    AATCATAGCA ACCACAGCAA CCATAGCAGC AGCAACAACA GCAAATCCCA ACCACCCACA
    ATAGGCTACG GCAGCAGTCA GCGACGCAGC GATGTGCCCA TGTATAATTG TGCAATGGAA
    GGCGCAACCA CAACATCTGC AACAGCAGAT ACGACAGCTG CCACATCGCG ATCGGCCGCA
    GCAAGCTCAC TGCAGATGCA ATTCGCTGCC GTCGCGGAGA GCAACAACGG CAGTAGCAGC
    GGCGGTGGTG GCGGCGGCGG CGGCTATGGC AGCATCGCTG GCGCTGGCGC CAACGCTGAT
    CCCCATCAGC CGTACAGCCA TGACCGCAAC AGCCTGCCGC CCGGCGTGGC TGACTGCGAT
    GCCAACAGCA ACAGCAGCAG TGTCACGCTG CAGGGCCTCG ATGCTCTCTT CAACGACATT
    GGCTCGGACT ACTTCAGCAA CGATATCGCC TTTGTGTCCA TCAATCCGCC GGACGACAAT
    GACTATCCGT ATGCGCTGAA CGCGAACGAG GGCATCGATC GGCTGTTTGA CTTCGGTTCG
    GATGCCTATG GACCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287455.1
    CDS971..3388
    Translation

    Target ORF information:

    RefSeq Version NM_001300526.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster E2F transcription factor 1 (E2f1), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCAAGT TCTTTGTGAA TGTTGCCCCC ATCAACAACA GCAACAGCAG CAGTAGCCAC 
    ACAACCACCA GCAGCAACAC CCAGCGCCAC CAGCAGCACC AACAACACTA TGGCGGGAGC
    GGGACAACCG GCCACACGAT GGTGGCCCGC AGACTCAACT ACGATCTGCA CGGCGGCACC
    ACCAGCATCA ACAACAACAA CAACATTGTG ATCAAGAACG AGTCCGTCGA CTTGGACTAC
    GATCACGTCC TGAGCAGCAG CGACAGCAAC AGCAACGGCG GCGTGGCAGC CCACCTGCGG
    GATCATGTCT ACATCAGTCT GGACAAGGGG CACAACACGG GGGCAGTGGC AACAGCAGCG
    GCGGCGGCAA CGGCGGGCCA CACGCAGCAG CAACTGCAGC AGCAGCACCA CCACCAAAAT
    CAGCAGCAAC GCAAGGCGAC CGGCAAATCC AACGATATCA CAAATTATTA CAAGGTCAAA
    CGTCGGCCAC ACGCCGTCAG CGATGAGATT CACCCGAAGA AGCAGGCCAA GCAGAGCGCC
    CACCACCAGA CGGTGTATCA GAAGCATACG GCTAGCAGTG CGCCCCAGCA GCTCCGGCAC
    AGCCACCACC AGCTGCGCCA CGACGCGGAT GCCGAACTGG ACGAGGATGT GGTGGAGCGG
    GTGGCCAAGC CGGCATCGCA TCACCCCTTT TCGCTGTCGA CGCCCCAGCA ACAGTTGGCT
    GCGTCGGTGG CCAGCAGTTC CTCGAGCGGA GATCGCAATC GGGCGGACAC CTCGCTGGGA
    ATACTGACCA AAAAGTTTGT GGACCTGCTG CAGGAGTCAC CGGATGGCGT GGTGGATCTG
    AACGAGGCCT CCAATCGATT GCACGTTCAG AAGCGGCGCA TCTACGACAT TACCAATGTC
    CTCGAAGGCA TTAACATCCT GGAGAAGAAG TCGAAGAACA ACATCCAGTG GCGCTGCGGG
    CAGTCGATGG TCAGCCAGGA GCGTTCCCGT CACATCGAAG CGGATTCGTT GCGGCTGGAG
    CAACAGGAGA ACGAGCTGAA CAAGGCCATC GATCTGATGC GCGAGAATCT GGCTGAGATC
    TCCCAGGAGG TGGAGAACTC CGGCGGCATG GCCTACGTCA CGCAGAACGA CCTGCTCAAC
    GTGGATCTCT TCAAAGATCA GATCGTTATC GTGATCAAGG CGCCGCCAGA GGCTAAGCTT
    GTGCTGCCCA ACACAAAATT GCCGCGCGAG ATTTACGTGA AGGCGGAGAA TAGCGGGGAG
    ATCAACGTCT TCCTCTGCCA TGACACCTCA CCGGAAAATT CGCCAATCGC GCCGGGCGCC
    GGCTACGTTG GAGCACCCGG TGCAGGATGC GTCCGTACGG CCACCTCCAC ACGACTGCAT
    CCGTTGACCA ACCAGCGACT GAACGATCCG CTCTTCAACA ATATCGATGC CATGAGCACC
    AAGGGATTAT TTCAAACGCC CTACCGCTCG GCTCGCAACC TCAGCAAATC GATTGAGGAA
    GCCGCCAAGC AGTCCCAGCC TGAATATAAT AACATTTGTG ATATTGCGAT GGGCCAGCAT
    CATAATCTGA ACCAGCAGCA GCAGCAGCAA CAACAACAGT TGCTACAGCA GCCGGAGGAG
    GACGATGTGG ACGTGGAGCT GAACCAGCTG GTTCCGACAC TGACGAATCC TGTGGTCCGG
    ACCCATCAGT TCCAGCAGCA CCAGCAGCCC TCCATCCAGG AGCTGTTCAG CAGCTTAACA
    GAATCCTCGC CTCCAACGCC CACCAAGCGG CGTCGGGAGG CAGCAGCCGC AGCAATTGCC
    GCAGGCAGCT CAACAACAGC AACCACTACG CTTAATAGTC ATAACAACAG AAACCACAGC
    AATCATAGCA ACCACAGCAA CCATAGCAGC AGCAACAACA GCAAATCCCA ACCACCCACA
    ATAGGCTACG GCAGCAGTCA GCGACGCAGC GATGTGCCCA TGTATAATTG TGCAATGGAA
    GGCGCAACCA CAACATCTGC AACAGCAGAT ACGACAGCTG CCACATCGCG ATCGGCCGCA
    GCAAGCTCAC TGCAGATGCA ATTCGCTGCC GTCGCGGAGA GCAACAACGG CAGTAGCAGC
    GGCGGTGGTG GCGGCGGCGG CGGCTATGGC AGCATCGCTG GCGCTGGCGC CAACGCTGAT
    CCCCATCAGC CGTACAGCCA TGACCGCAAC AGCCTGCCGC CCGGCGTGGC TGACTGCGAT
    GCCAACAGCA ACAGCAGCAG TGTCACGCTG CAGGGCCTCG ATGCTCTCTT CAACGACATT
    GGCTCGGACT ACTTCAGCAA CGATATCGCC TTTGTGTCCA TCAATCCGCC GGACGACAAT
    GACTATCCGT ATGCGCTGAA CGCGAACGAG GGCATCGATC GGCTGTTTGA CTTCGGTTCG
    GATGCCTATG GACCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08624
    Clone ID Related Accession (Same CDS sequence) NM_001275880.2 , NM_001300525.1
    Accession Version NM_001275880.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product E2F transcription factor 1, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275880.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCAAGT TCTTTGTGAA TGTTGCCCCC ATCAACAACA GCAACAGCAG CAGTAGCCAC 
    ACAACCACCA GCAGCAACAC CCAGCGCCAC CAGCAGCACC AACAACACTA TGGCGGGAGC
    GGGACAACCG GCCACACGAT GGTGGCCCGC AGACTCAACT ACGATCTGCA CGGCGGCACC
    ACCAGCATCA ACAACAACAA CAACATTGTG ATCAAGAACG AGTCCGTCGA CTTGGACTAC
    GATCACGTCC TGAGCAGCAG CGACAGCAAC AGCAACGGCG GCGTGGCAGC CCACCTGCGG
    GATCATGTCT ACATCAGTCT GGACAAGGGG CACAACACGG GGGCAGTGGC AACAGCAGCG
    GCGGCGGCAA CGGCGGGCCA CACGCAGCAG CAACTGCAGC AGCAGCACCA CCACCAAAAT
    CAGCAGCAAC GCAAGGCGAC CGGCAAATCC AACGATATCA CAAATTATTA CAAGGTCAAA
    CGTCGGCCAC ACGCCGTCAG CGATGAGATT CACCCGAAGA AGCAGGCCAA GCAGAGCGCC
    CACCACCAGA CGGTGTATCA GAAGCATACG GCTAGCAGTG CGCCCCAGCA GCTCCGGCAC
    AGCCACCACC AGCTGCGCCA CGACGCGGAT GCCGAACTGG ACGAGGATGT GGTGGAGCGG
    GTGGCCAAGC CGGCATCGCA TCACCCCTTT TCGCTGTCGA CGCCCCAGCA ACAGTTGGCT
    GCGTCGGTGG CCAGCAGTTC CTCGAGCGGA GATCGCAATC GGGCGGACAC CTCGCTGGGA
    ATACTGACCA AAAAGTTTGT GGACCTGCTG CAGGAGTCAC CGGATGGCGT GGTGGATCTG
    AACGAGGCCT CCAATCGATT GCACGTTCAG AAGCGGCGCA TCTACGACAT TACCAATGTC
    CTCGAAGGCA TTAACATCCT GGAGAAGAAG TCGAAGAACA ACATCCAGTG GCGCTGCGGG
    CAGTCGATGG TCAGCCAGGA GCGTTCCCGT CACATCGAAG CGGATTCGTT GCGGCTGGAG
    CAACAGGAGA ACGAGCTGAA CAAGGCCATC GATCTGATGC GCGAGAATCT GGCTGAGATC
    TCCCAGGAGG TGGAGAACTC CGGCGGCATG GCCTACGTCA CGCAGAACGA CCTGCTCAAC
    GTGGATCTCT TCAAAGATCA GATCGTTATC GTGATCAAGG CGCCGCCAGA GGCTAAGCTT
    GTGCGGTCGG CGACCGCTTT AAAATTTCAA CCGCTCCCCT TCAATTTGAA CCTGCCCAAC
    ACAAAATTGC CGCGCGAGAT TTACGTGAAG GCGGAGAATA GCGGGGAGAT CAACGTCTTC
    CTCTGCCATG ACACCTCACC GGAAAATTCG CCAATCGCGC CGGGCGCCGG CTACGTTGGA
    GCACCCGGTG CAGGATGCGT CCGTACGGCC ACCTCCACAC GACTGCATCC GTTGACCAAC
    CAGCGACTGA ACGATCCGCT CTTCAACAAT ATCGATGCCA TGAGCACCAA GGGATTATTT
    CAAACGCCCT ACCGCTCGGC TCGCAACCTC AGCAAATCGA TTGAGGAAGC CGCCAAGCAG
    TCCCAGCCTG AATATAATAA CATTTGTGAT ATTGCGATGG GCCAGCATCA TAATCTGAAC
    CAGCAGCAGC AGCAGCAACA ACAACAGTTG CTACAGCAGC CGGAGGAGGA CGATGTGGAC
    GTGGAGCTGA ACCAGCTGGT TCCGACACTG ACGAATCCTG TGGTCCGGAC CCATCAGTTC
    CAGCAGCACC AGCAGCCCTC CATCCAGGAG CTGTTCAGCA GCTTAACAGA ATCCTCGCCT
    CCAACGCCCA CCAAGCGGCG TCGGGAGGCA GCAGCCGCAG CAATTGCCGC AGGCAGCTCA
    ACAACAGCAA CCACTACGCT TAATAGTCAT AACAACAGAA ACCACAGCAA TCATAGCAAC
    CACAGCAACC ATAGCAGCAG CAACAACAGC AAATCCCAAC CACCCACAAT AGGCTACGGC
    AGCAGTCAGC GACGCAGCGA TGTGCCCATG TATAATTGTG CAATGGAAGG CGCAACCACA
    ACATCTGCAA CAGCAGATAC GACAGCTGCC ACATCGCGAT CGGCCGCAGC AAGCTCACTG
    CAGATGCAAT TCGCTGCCGT CGCGGAGAGC AACAACGGCA GTAGCAGCGG CGGTGGTGGC
    GGCGGCGGCG GCTATGGCAG CATCGCTGGC GCTGGCGCCA ACGCTGATCC CCATCAGCCG
    TACAGCCATG ACCGCAACAG CCTGCCGCCC GGCGTGGCTG ACTGCGATGC CAACAGCAAC
    AGCAGCAGTG TCACGCTGCA GGGCCTCGAT GCTCTCTTCA ACGACATTGG CTCGGACTAC
    TTCAGCAACG ATATCGCCTT TGTGTCCATC AATCCGCCGG ACGACAATGA CTATCCGTAT
    GCGCTGAACG CGAACGAGGG CATCGATCGG CTGTTTGACT TCGGTTCGGA TGCCTATGGA
    CCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262809.1
    CDS915..3380
    Translation

    Target ORF information:

    RefSeq Version NM_001275880.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster E2F transcription factor 1 (E2f1), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275880.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCAAGT TCTTTGTGAA TGTTGCCCCC ATCAACAACA GCAACAGCAG CAGTAGCCAC 
    ACAACCACCA GCAGCAACAC CCAGCGCCAC CAGCAGCACC AACAACACTA TGGCGGGAGC
    GGGACAACCG GCCACACGAT GGTGGCCCGC AGACTCAACT ACGATCTGCA CGGCGGCACC
    ACCAGCATCA ACAACAACAA CAACATTGTG ATCAAGAACG AGTCCGTCGA CTTGGACTAC
    GATCACGTCC TGAGCAGCAG CGACAGCAAC AGCAACGGCG GCGTGGCAGC CCACCTGCGG
    GATCATGTCT ACATCAGTCT GGACAAGGGG CACAACACGG GGGCAGTGGC AACAGCAGCG
    GCGGCGGCAA CGGCGGGCCA CACGCAGCAG CAACTGCAGC AGCAGCACCA CCACCAAAAT
    CAGCAGCAAC GCAAGGCGAC CGGCAAATCC AACGATATCA CAAATTATTA CAAGGTCAAA
    CGTCGGCCAC ACGCCGTCAG CGATGAGATT CACCCGAAGA AGCAGGCCAA GCAGAGCGCC
    CACCACCAGA CGGTGTATCA GAAGCATACG GCTAGCAGTG CGCCCCAGCA GCTCCGGCAC
    AGCCACCACC AGCTGCGCCA CGACGCGGAT GCCGAACTGG ACGAGGATGT GGTGGAGCGG
    GTGGCCAAGC CGGCATCGCA TCACCCCTTT TCGCTGTCGA CGCCCCAGCA ACAGTTGGCT
    GCGTCGGTGG CCAGCAGTTC CTCGAGCGGA GATCGCAATC GGGCGGACAC CTCGCTGGGA
    ATACTGACCA AAAAGTTTGT GGACCTGCTG CAGGAGTCAC CGGATGGCGT GGTGGATCTG
    AACGAGGCCT CCAATCGATT GCACGTTCAG AAGCGGCGCA TCTACGACAT TACCAATGTC
    CTCGAAGGCA TTAACATCCT GGAGAAGAAG TCGAAGAACA ACATCCAGTG GCGCTGCGGG
    CAGTCGATGG TCAGCCAGGA GCGTTCCCGT CACATCGAAG CGGATTCGTT GCGGCTGGAG
    CAACAGGAGA ACGAGCTGAA CAAGGCCATC GATCTGATGC GCGAGAATCT GGCTGAGATC
    TCCCAGGAGG TGGAGAACTC CGGCGGCATG GCCTACGTCA CGCAGAACGA CCTGCTCAAC
    GTGGATCTCT TCAAAGATCA GATCGTTATC GTGATCAAGG CGCCGCCAGA GGCTAAGCTT
    GTGCGGTCGG CGACCGCTTT AAAATTTCAA CCGCTCCCCT TCAATTTGAA CCTGCCCAAC
    ACAAAATTGC CGCGCGAGAT TTACGTGAAG GCGGAGAATA GCGGGGAGAT CAACGTCTTC
    CTCTGCCATG ACACCTCACC GGAAAATTCG CCAATCGCGC CGGGCGCCGG CTACGTTGGA
    GCACCCGGTG CAGGATGCGT CCGTACGGCC ACCTCCACAC GACTGCATCC GTTGACCAAC
    CAGCGACTGA ACGATCCGCT CTTCAACAAT ATCGATGCCA TGAGCACCAA GGGATTATTT
    CAAACGCCCT ACCGCTCGGC TCGCAACCTC AGCAAATCGA TTGAGGAAGC CGCCAAGCAG
    TCCCAGCCTG AATATAATAA CATTTGTGAT ATTGCGATGG GCCAGCATCA TAATCTGAAC
    CAGCAGCAGC AGCAGCAACA ACAACAGTTG CTACAGCAGC CGGAGGAGGA CGATGTGGAC
    GTGGAGCTGA ACCAGCTGGT TCCGACACTG ACGAATCCTG TGGTCCGGAC CCATCAGTTC
    CAGCAGCACC AGCAGCCCTC CATCCAGGAG CTGTTCAGCA GCTTAACAGA ATCCTCGCCT
    CCAACGCCCA CCAAGCGGCG TCGGGAGGCA GCAGCCGCAG CAATTGCCGC AGGCAGCTCA
    ACAACAGCAA CCACTACGCT TAATAGTCAT AACAACAGAA ACCACAGCAA TCATAGCAAC
    CACAGCAACC ATAGCAGCAG CAACAACAGC AAATCCCAAC CACCCACAAT AGGCTACGGC
    AGCAGTCAGC GACGCAGCGA TGTGCCCATG TATAATTGTG CAATGGAAGG CGCAACCACA
    ACATCTGCAA CAGCAGATAC GACAGCTGCC ACATCGCGAT CGGCCGCAGC AAGCTCACTG
    CAGATGCAAT TCGCTGCCGT CGCGGAGAGC AACAACGGCA GTAGCAGCGG CGGTGGTGGC
    GGCGGCGGCG GCTATGGCAG CATCGCTGGC GCTGGCGCCA ACGCTGATCC CCATCAGCCG
    TACAGCCATG ACCGCAACAG CCTGCCGCCC GGCGTGGCTG ACTGCGATGC CAACAGCAAC
    AGCAGCAGTG TCACGCTGCA GGGCCTCGAT GCTCTCTTCA ACGACATTGG CTCGGACTAC
    TTCAGCAACG ATATCGCCTT TGTGTCCATC AATCCGCCGG ACGACAATGA CTATCCGTAT
    GCGCTGAACG CGAACGAGGG CATCGATCGG CTGTTTGACT TCGGTTCGGA TGCCTATGGA
    CCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08624
    Clone ID Related Accession (Same CDS sequence) NM_001275880.2 , NM_001300525.1
    Accession Version NM_001300525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product E2F transcription factor 1, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCAAGT TCTTTGTGAA TGTTGCCCCC ATCAACAACA GCAACAGCAG CAGTAGCCAC 
    ACAACCACCA GCAGCAACAC CCAGCGCCAC CAGCAGCACC AACAACACTA TGGCGGGAGC
    GGGACAACCG GCCACACGAT GGTGGCCCGC AGACTCAACT ACGATCTGCA CGGCGGCACC
    ACCAGCATCA ACAACAACAA CAACATTGTG ATCAAGAACG AGTCCGTCGA CTTGGACTAC
    GATCACGTCC TGAGCAGCAG CGACAGCAAC AGCAACGGCG GCGTGGCAGC CCACCTGCGG
    GATCATGTCT ACATCAGTCT GGACAAGGGG CACAACACGG GGGCAGTGGC AACAGCAGCG
    GCGGCGGCAA CGGCGGGCCA CACGCAGCAG CAACTGCAGC AGCAGCACCA CCACCAAAAT
    CAGCAGCAAC GCAAGGCGAC CGGCAAATCC AACGATATCA CAAATTATTA CAAGGTCAAA
    CGTCGGCCAC ACGCCGTCAG CGATGAGATT CACCCGAAGA AGCAGGCCAA GCAGAGCGCC
    CACCACCAGA CGGTGTATCA GAAGCATACG GCTAGCAGTG CGCCCCAGCA GCTCCGGCAC
    AGCCACCACC AGCTGCGCCA CGACGCGGAT GCCGAACTGG ACGAGGATGT GGTGGAGCGG
    GTGGCCAAGC CGGCATCGCA TCACCCCTTT TCGCTGTCGA CGCCCCAGCA ACAGTTGGCT
    GCGTCGGTGG CCAGCAGTTC CTCGAGCGGA GATCGCAATC GGGCGGACAC CTCGCTGGGA
    ATACTGACCA AAAAGTTTGT GGACCTGCTG CAGGAGTCAC CGGATGGCGT GGTGGATCTG
    AACGAGGCCT CCAATCGATT GCACGTTCAG AAGCGGCGCA TCTACGACAT TACCAATGTC
    CTCGAAGGCA TTAACATCCT GGAGAAGAAG TCGAAGAACA ACATCCAGTG GCGCTGCGGG
    CAGTCGATGG TCAGCCAGGA GCGTTCCCGT CACATCGAAG CGGATTCGTT GCGGCTGGAG
    CAACAGGAGA ACGAGCTGAA CAAGGCCATC GATCTGATGC GCGAGAATCT GGCTGAGATC
    TCCCAGGAGG TGGAGAACTC CGGCGGCATG GCCTACGTCA CGCAGAACGA CCTGCTCAAC
    GTGGATCTCT TCAAAGATCA GATCGTTATC GTGATCAAGG CGCCGCCAGA GGCTAAGCTT
    GTGCGGTCGG CGACCGCTTT AAAATTTCAA CCGCTCCCCT TCAATTTGAA CCTGCCCAAC
    ACAAAATTGC CGCGCGAGAT TTACGTGAAG GCGGAGAATA GCGGGGAGAT CAACGTCTTC
    CTCTGCCATG ACACCTCACC GGAAAATTCG CCAATCGCGC CGGGCGCCGG CTACGTTGGA
    GCACCCGGTG CAGGATGCGT CCGTACGGCC ACCTCCACAC GACTGCATCC GTTGACCAAC
    CAGCGACTGA ACGATCCGCT CTTCAACAAT ATCGATGCCA TGAGCACCAA GGGATTATTT
    CAAACGCCCT ACCGCTCGGC TCGCAACCTC AGCAAATCGA TTGAGGAAGC CGCCAAGCAG
    TCCCAGCCTG AATATAATAA CATTTGTGAT ATTGCGATGG GCCAGCATCA TAATCTGAAC
    CAGCAGCAGC AGCAGCAACA ACAACAGTTG CTACAGCAGC CGGAGGAGGA CGATGTGGAC
    GTGGAGCTGA ACCAGCTGGT TCCGACACTG ACGAATCCTG TGGTCCGGAC CCATCAGTTC
    CAGCAGCACC AGCAGCCCTC CATCCAGGAG CTGTTCAGCA GCTTAACAGA ATCCTCGCCT
    CCAACGCCCA CCAAGCGGCG TCGGGAGGCA GCAGCCGCAG CAATTGCCGC AGGCAGCTCA
    ACAACAGCAA CCACTACGCT TAATAGTCAT AACAACAGAA ACCACAGCAA TCATAGCAAC
    CACAGCAACC ATAGCAGCAG CAACAACAGC AAATCCCAAC CACCCACAAT AGGCTACGGC
    AGCAGTCAGC GACGCAGCGA TGTGCCCATG TATAATTGTG CAATGGAAGG CGCAACCACA
    ACATCTGCAA CAGCAGATAC GACAGCTGCC ACATCGCGAT CGGCCGCAGC AAGCTCACTG
    CAGATGCAAT TCGCTGCCGT CGCGGAGAGC AACAACGGCA GTAGCAGCGG CGGTGGTGGC
    GGCGGCGGCG GCTATGGCAG CATCGCTGGC GCTGGCGCCA ACGCTGATCC CCATCAGCCG
    TACAGCCATG ACCGCAACAG CCTGCCGCCC GGCGTGGCTG ACTGCGATGC CAACAGCAAC
    AGCAGCAGTG TCACGCTGCA GGGCCTCGAT GCTCTCTTCA ACGACATTGG CTCGGACTAC
    TTCAGCAACG ATATCGCCTT TGTGTCCATC AATCCGCCGG ACGACAATGA CTATCCGTAT
    GCGCTGAACG CGAACGAGGG CATCGATCGG CTGTTTGACT TCGGTTCGGA TGCCTATGGA
    CCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287454.1
    CDS504..2969
    Translation

    Target ORF information:

    RefSeq Version NM_001300525.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster E2F transcription factor 1 (E2f1), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCAAGT TCTTTGTGAA TGTTGCCCCC ATCAACAACA GCAACAGCAG CAGTAGCCAC 
    ACAACCACCA GCAGCAACAC CCAGCGCCAC CAGCAGCACC AACAACACTA TGGCGGGAGC
    GGGACAACCG GCCACACGAT GGTGGCCCGC AGACTCAACT ACGATCTGCA CGGCGGCACC
    ACCAGCATCA ACAACAACAA CAACATTGTG ATCAAGAACG AGTCCGTCGA CTTGGACTAC
    GATCACGTCC TGAGCAGCAG CGACAGCAAC AGCAACGGCG GCGTGGCAGC CCACCTGCGG
    GATCATGTCT ACATCAGTCT GGACAAGGGG CACAACACGG GGGCAGTGGC AACAGCAGCG
    GCGGCGGCAA CGGCGGGCCA CACGCAGCAG CAACTGCAGC AGCAGCACCA CCACCAAAAT
    CAGCAGCAAC GCAAGGCGAC CGGCAAATCC AACGATATCA CAAATTATTA CAAGGTCAAA
    CGTCGGCCAC ACGCCGTCAG CGATGAGATT CACCCGAAGA AGCAGGCCAA GCAGAGCGCC
    CACCACCAGA CGGTGTATCA GAAGCATACG GCTAGCAGTG CGCCCCAGCA GCTCCGGCAC
    AGCCACCACC AGCTGCGCCA CGACGCGGAT GCCGAACTGG ACGAGGATGT GGTGGAGCGG
    GTGGCCAAGC CGGCATCGCA TCACCCCTTT TCGCTGTCGA CGCCCCAGCA ACAGTTGGCT
    GCGTCGGTGG CCAGCAGTTC CTCGAGCGGA GATCGCAATC GGGCGGACAC CTCGCTGGGA
    ATACTGACCA AAAAGTTTGT GGACCTGCTG CAGGAGTCAC CGGATGGCGT GGTGGATCTG
    AACGAGGCCT CCAATCGATT GCACGTTCAG AAGCGGCGCA TCTACGACAT TACCAATGTC
    CTCGAAGGCA TTAACATCCT GGAGAAGAAG TCGAAGAACA ACATCCAGTG GCGCTGCGGG
    CAGTCGATGG TCAGCCAGGA GCGTTCCCGT CACATCGAAG CGGATTCGTT GCGGCTGGAG
    CAACAGGAGA ACGAGCTGAA CAAGGCCATC GATCTGATGC GCGAGAATCT GGCTGAGATC
    TCCCAGGAGG TGGAGAACTC CGGCGGCATG GCCTACGTCA CGCAGAACGA CCTGCTCAAC
    GTGGATCTCT TCAAAGATCA GATCGTTATC GTGATCAAGG CGCCGCCAGA GGCTAAGCTT
    GTGCGGTCGG CGACCGCTTT AAAATTTCAA CCGCTCCCCT TCAATTTGAA CCTGCCCAAC
    ACAAAATTGC CGCGCGAGAT TTACGTGAAG GCGGAGAATA GCGGGGAGAT CAACGTCTTC
    CTCTGCCATG ACACCTCACC GGAAAATTCG CCAATCGCGC CGGGCGCCGG CTACGTTGGA
    GCACCCGGTG CAGGATGCGT CCGTACGGCC ACCTCCACAC GACTGCATCC GTTGACCAAC
    CAGCGACTGA ACGATCCGCT CTTCAACAAT ATCGATGCCA TGAGCACCAA GGGATTATTT
    CAAACGCCCT ACCGCTCGGC TCGCAACCTC AGCAAATCGA TTGAGGAAGC CGCCAAGCAG
    TCCCAGCCTG AATATAATAA CATTTGTGAT ATTGCGATGG GCCAGCATCA TAATCTGAAC
    CAGCAGCAGC AGCAGCAACA ACAACAGTTG CTACAGCAGC CGGAGGAGGA CGATGTGGAC
    GTGGAGCTGA ACCAGCTGGT TCCGACACTG ACGAATCCTG TGGTCCGGAC CCATCAGTTC
    CAGCAGCACC AGCAGCCCTC CATCCAGGAG CTGTTCAGCA GCTTAACAGA ATCCTCGCCT
    CCAACGCCCA CCAAGCGGCG TCGGGAGGCA GCAGCCGCAG CAATTGCCGC AGGCAGCTCA
    ACAACAGCAA CCACTACGCT TAATAGTCAT AACAACAGAA ACCACAGCAA TCATAGCAAC
    CACAGCAACC ATAGCAGCAG CAACAACAGC AAATCCCAAC CACCCACAAT AGGCTACGGC
    AGCAGTCAGC GACGCAGCGA TGTGCCCATG TATAATTGTG CAATGGAAGG CGCAACCACA
    ACATCTGCAA CAGCAGATAC GACAGCTGCC ACATCGCGAT CGGCCGCAGC AAGCTCACTG
    CAGATGCAAT TCGCTGCCGT CGCGGAGAGC AACAACGGCA GTAGCAGCGG CGGTGGTGGC
    GGCGGCGGCG GCTATGGCAG CATCGCTGGC GCTGGCGCCA ACGCTGATCC CCATCAGCCG
    TACAGCCATG ACCGCAACAG CCTGCCGCCC GGCGTGGCTG ACTGCGATGC CAACAGCAAC
    AGCAGCAGTG TCACGCTGCA GGGCCTCGAT GCTCTCTTCA ACGACATTGG CTCGGACTAC
    TTCAGCAACG ATATCGCCTT TGTGTCCATC AATCCGCCGG ACGACAATGA CTATCCGTAT
    GCGCTGAACG CGAACGAGGG CATCGATCGG CTGTTTGACT TCGGTTCGGA TGCCTATGGA
    CCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.