tin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tin
Entrez Gene ID	42536
Full Name	tinman
Synonyms	CG7895,DROHOXHK4,DROHOXNK4,DmNK-4,Dmel\CG7895,HOX,NK-4,NK-4/msh-2,NK4,NK4/msh-2,NKX2.5,TIN,Tin,msh-2,msh2,tin/NK4
General protein information	Preferred Names tinman Names CG7895 gene product from transcript CG7895-RA CG7895-PA muscle-specific-homeodomain-2 tin-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 93D9-93D9

mRNA and Protein(s)

mRNA	Protein	Name
NM_079709.3	NP_524433.1	tinman

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003670	XP_562540.3
Drosophila melanogaster (fruit fly)	tin	NP_524433.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings provide mechanistic insight into the brake on myogenesis that occurs during mesoderm specification: twist and tin expression at early stages in turn activate the myogenic inhibitor Him; yet, once Twist or Tin levels decline at mid-embryogenesis, Him is no longer expressed in the mesoderm, and MEF2-dependent muscle differentiation can proceed.
Title: The myogenic repressor gene Holes in muscles is a direct transcriptional target of Twist and Tinman in the Drosophila embryonic mesoderm.

Overexpression of Tinman and Pannier resulted in 20% of embryos with ectopic Hand and Sur expression. By adding MEF2 alongside Tinman and Pannier, an expansion in expression of Hand and Sur was observed.
Title: The Drosophila Transcription Factors Tinman and Pannier Activate and Collaborate with Myocyte Enhancer Factor-2 to Promote Heart Cell Fate.

Plasticity in Hox/PBC interaction modes is a common molecular strategy for shaping Hox transcriptional activities.
Title: Hox transcriptional specificity despite a single class of cofactors: are flexible interaction modes the key? Plasticity in Hox/PBC interaction modes as a common molecular strategy for shaping Hox transcriptional activities.

The enhancers active in the heart progenitor cells and the heart generally are dependent on tinman gene activity, whereas those active in non-cardiac mesoderm are often bound neutrally by Tinman
Title: Genome-wide screens for in vivo Tinman binding sites identify cardiac enhancers with diverse functional architectures.

Genetic interaction analysis shows that spir functionally interacts with Dorsocross, tin, and pannier to properly specify the cardiac fate.
Title: Spire, an actin nucleation factor, regulates cell division during Drosophila heart development.

The following tin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05596	NM_079709.3 Latest version!	Drosophila melanogaster tinman (tin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tin ( NM_079709.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524433.1 Drosophila melanogaster tinman (tin), mRNA.

Price & Availability↑

CloneID	OFa05596
Clone ID Related Accession (Same CDS sequence)	NM_079709.3
Accession Version	NM_079709.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tinman
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079709.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCAGC ACCATCAGCA GCAGGCTCAA TCGGGTGGCT ACTACGATCA CTACACTCAG 
TCCCCCAGTC CGGGCAGCCT GACGAACGCG GATGCCCTGA ACACCACTCC ATTTTCGGTC 
AAGGATATCC TGAACATGGT CAACCAAACG GAGGCCTACG AAGGTTCCTA CGGGCATATC 
GATGGCGCGG CGACTGCCTC GGCGCTCTTC GCCGCCGGTG AGTACCAGAA TCCCCACCAG 
TACCTCAACC ACCAGCAGCA TCAGCAATCC GAGCTGCCGA TTCCGCAGCA GCAACTGCAT 
CACCAACACT TGGACGATGG CGCCACCACA TCGTCGTCCC TATCGCCACT CTTGCCACCC 
CCGCCGCACC AGCTGTACGG CGGATACCAG GACTACGGTA TGCCCGCCCA CATGTTCCAG 
CACCATCACG GACATCCCCA CCAGAGTTTC CAGCACTCCG CCTCGGCCTA CAACATGTCC 
GCCTCGCAGT TCTACGCCGG CGCCTCGGCC ACGGCCTACC AGACCCCCGC CACCTACAAC 
TACAACTACG CCGGATCTGG TGAGGTGTAC GGTGGAGCCA CGCCCTCGGC GGTGGGCATC 
AAGAGCGAGT ACATACCCAC GCCATACGTC ACGCCCTCGC CCACCCTGGA CCTCAACAGC 
TCCGCTGAGG TGGACAGCCT GCAAGCTCCG ACGCAGAAGC TGTGCGTCAA TCCCCTGTCG 
CAGCGCCTCA TGGAGACCGC TAGCAACTCG TCCTCCCTAA GGAGCATCTA CGGTTCAGAT 
GAGGGCGCCA AAAAAAAGGA TAACAGCCAG GTGACCTCCT CGCGTTCCGA GCTGCGAAAA 
AACAGCATCA GTGGGAACAG CAATCCGGGG AGCAACAGTG GTTCCACCAA GCCCCGGATG 
AAGCGAAAGC CTCGCGTGCT CTTTTCCCAG GCACAGGTCC TGGAGCTGGA GTGTCGCTTT 
CGACTCAAAA AGTATCTGAC GGGTGCGGAG CGCGAGATAA TCGCGCAAAA GCTTAACCTG 
TCGGCCACCC AAGTGAAGAT TTGGTTCCAG AATCGGCGCT ACAAATCGAA ACGTGGCGAC 
ATCGACTGCG AGGGCATCGC CAAGCATCTG AAGTTGAAGT CCGAGCCCCT GGACTCGCCC 
ACTTCTCTGC CCCCGCCGAT TCCCAACCAC GTGATGTGGC CCCCAACCAT GCAGCAATCG 
CAGCAGCAGC AGCAGCATCA TGCACAGCAG CAACAGATGC AGCACATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524433.1
CDS	271..1521
Translation	MLQHHQQQAQSGGYYDHYTQSPSPGSLTNADALNTTPFSVKDILNMVNQTEAYEGSYGHIDGAATASALFAAGEYQNPHQYLNHQQHQQSELPIPQQQLHHQHLDDGATTSSSLSPLLPPPPHQLYGGYQDYGMPAHMFQHHHGHPHQSFQHSASAYNMSASQFYAGASATAYQTPATYNYNYAGSGEVYGGATPSAVGIKSEYIPTPYVTPSPTLDLNSSAEVDSLQAPTQKLCVNPLSQRLMETASNSSSLRSIYGSDEGAKKKDNSQVTSSRSELRKNSISGNSNPGSNSGSTKPRMKRKPRVLFSQAQVLELECRFRLKKYLTGAEREIIAQKLNLSATQVKIWFQNRRYKSKRGDIDCEGIAKHLKLKSEPLDSPTSLPPPIPNHVMWPPTMQQSQQQQQHHAQQQQMQHM

Target ORF information:

RefSeq Version	NM_079709.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tinman (tin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079709.3

ORF Insert Sequence:

ATGTTGCAGC ACCATCAGCA GCAGGCTCAA TCGGGTGGCT ACTACGATCA CTACACTCAG 
TCCCCCAGTC CGGGCAGCCT GACGAACGCG GATGCCCTGA ACACCACTCC ATTTTCGGTC 
AAGGATATCC TGAACATGGT CAACCAAACG GAGGCCTACG AAGGTTCCTA CGGGCATATC 
GATGGCGCGG CGACTGCCTC GGCGCTCTTC GCCGCCGGTG AGTACCAGAA TCCCCACCAG 
TACCTCAACC ACCAGCAGCA TCAGCAATCC GAGCTGCCGA TTCCGCAGCA GCAACTGCAT 
CACCAACACT TGGACGATGG CGCCACCACA TCGTCGTCCC TATCGCCACT CTTGCCACCC 
CCGCCGCACC AGCTGTACGG CGGATACCAG GACTACGGTA TGCCCGCCCA CATGTTCCAG 
CACCATCACG GACATCCCCA CCAGAGTTTC CAGCACTCCG CCTCGGCCTA CAACATGTCC 
GCCTCGCAGT TCTACGCCGG CGCCTCGGCC ACGGCCTACC AGACCCCCGC CACCTACAAC 
TACAACTACG CCGGATCTGG TGAGGTGTAC GGTGGAGCCA CGCCCTCGGC GGTGGGCATC 
AAGAGCGAGT ACATACCCAC GCCATACGTC ACGCCCTCGC CCACCCTGGA CCTCAACAGC 
TCCGCTGAGG TGGACAGCCT GCAAGCTCCG ACGCAGAAGC TGTGCGTCAA TCCCCTGTCG 
CAGCGCCTCA TGGAGACCGC TAGCAACTCG TCCTCCCTAA GGAGCATCTA CGGTTCAGAT 
GAGGGCGCCA AAAAAAAGGA TAACAGCCAG GTGACCTCCT CGCGTTCCGA GCTGCGAAAA 
AACAGCATCA GTGGGAACAG CAATCCGGGG AGCAACAGTG GTTCCACCAA GCCCCGGATG 
AAGCGAAAGC CTCGCGTGCT CTTTTCCCAG GCACAGGTCC TGGAGCTGGA GTGTCGCTTT 
CGACTCAAAA AGTATCTGAC GGGTGCGGAG CGCGAGATAA TCGCGCAAAA GCTTAACCTG 
TCGGCCACCC AAGTGAAGAT TTGGTTCCAG AATCGGCGCT ACAAATCGAA ACGTGGCGAC 
ATCGACTGCG AGGGCATCGC CAAGCATCTG AAGTTGAAGT CCGAGCCCCT GGACTCGCCC 
ACTTCTCTGC CCCCGCCGAT TCCCAACCAC GTGATGTGGC CCCCAACCAT GCAGCAATCG 
CAGCAGCAGC AGCAGCATCA TGCACAGCAG CAACAGATGC AGCACATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

tin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報