e cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	e
Entrez Gene ID	42521
Full Name	ebony
Synonyms	CG3331,Dmel\CG3331
General protein information	Preferred Names ebony Names CG3331 gene product from transcript CG3331-RA CG3331-PA N-betagr;-alanyl-dopamine synthetase NBAD-synthase e-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 93C7-93D1\|3-70.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079707.4	NP_524431.2	ebony

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002291	XP_307887.5
Kluyveromyces lactis	KLLA0B09218g	XP_451939.1
Neurospora crassa	NCU03010	XP_965396.1
Bos taurus (cattle)	LOC101902015	XP_005201086.1
Saccharomyces cerevisiae (baker's yeast)	LYS2	NP_009673.1
Drosophila melanogaster (fruit fly)	e	NP_524431.2
Schizosaccharomyces pombe (fission yeast)	lys1	NP_594314.1
Magnaporthe oryzae (rice blast fungus)	MGG_02611	XP_003721184.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ebony is a novel type of nonribosomal peptide synthetase related molecule that combines a slow amino acid activation phase with a very fast product formation step.
Title: Drosophila Ebony: a novel type of nonribosomal peptide synthetase related enzyme with unusually fast peptide bond formation kinetics.

optimal binding ratio between Dysb and Ebony might contribute to such non-linear effects. Thus, Ddysb-dependent regulation of Ebony could be one of the mechanisms that mediate dopamine signal
Title: The ?-alanyl-monoamine synthase ebony is regulated by schizophrenia susceptibility gene dysbindin in Drosophila.

Activity, localization and strict coexpression of Ebony with Black protein is specific to the optic lobes.
Title: Activity and coexpression of Drosophila black with ebony in fly optic lobes reveals putative cooperative tasks in vision that evade electroretinographic detection.

Ddysb regulates glutamatergic transmission through its neuronal function and regulates dopamine metabolism by regulating Ebony expression in glial cells
Title: Schizophrenia susceptibility gene dysbindin regulates glutamatergic and dopaminergic functions via distinctive mechanisms in Drosophila.

Nevertheless, both adaptive changes involved cis-regulatory changes of ebony, which shows that this gene is likely to be a common target of natural selection.
Title: Divergent enhancer haplotype of ebony on inversion In(3R)Payne associated with pigmentation variation in a tropical population of Drosophila melanogaster.

The following e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05583	NM_079707.4 Latest version!	Drosophila melanogaster ebony (e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

e ( NM_079707.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524431.2 Drosophila melanogaster ebony (e), mRNA.

Price & Availability↑

CloneID	OFa05583
Clone ID Related Accession (Same CDS sequence)	NM_079707.4
Accession Version	NM_079707.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ebony
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079707.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCGC TGCCACAATT GTCGATCGTC AAGGGTCTGC AGCAAGACTT CGTGCCTAGA 
GCTCTGCACC GCATCTTCGA GGAGCAGCAG CTGCGGCATG CCGACAAGGT GGCTCTGATC 
TATCAGCCCA GCACTACGGG CCAGGGAATG GCGCCCAGTC AGAGCAGCTA CCGCCAGATG 
AACGAGCGTG CGAACCGGGC AGCCCGCCTC CTGGTGGCCG AGACCCACGG CCGGTTCCTG 
CAGCCAAACA GCGATGGTGA CTTCATCGTG GCTGTGTGCA TGCAGCCGTC GGAGGGATTG 
GTCACCACAC TGCTGGCCAT CTGGAAGGCT GGCGGCGCGT ATTTGCCCAT CGATCCCAGC 
TTCCCGGCGA ACCGCATTCA CCACATACTG CTGGAGGCGA AGCCCACCTT GGTGATTCGC 
GACGATGACA TCGACGCCGG CCGTTTCCAG GGAACTCCCA CGTTATCCAC CACCGAACTG 
TATGCCAAAT CCCTCCAGCT GGCCGGCTCC AATCTGCTCT CAGAGGAGAT GCTGCGCGGT 
GGCAACGACC ACATCGCCAT CGTGCTCTAC ACCTCGGGCA GTACGGGTGT GCCCAAGGGA 
GTGCGTCTGC CGCACGAGAG CATCCTCAAT CGGCTCCAGT GGCAGTGGGC GACCTTTCCG 
TACACCGCCA ACGAGGCGGT GAGCGTTTTC AAGACGGCCC TAACCTTCGT GGACTCGATT 
GCGGAGCTAT GGGGCCCGTT AATGTGTGGC CTGGCCATTC TGGTGGTGCC CAAGGCAGTG 
ACCAAGGATC CCCAGCGATT GGTGGCCCTG TTGGAGCGTT ATAAGATCAG GCGTCTCGTA 
CTGGTGCCCA CCCTTCTGCG CTCGTTGCTC ATGTATCTGA AGATGGAGGG AGGCGGAGCT 
GCTCAGAAAC TGCTGTACAA TCTCCAGATT TGGGTCTGCT CCGGAGAACC CCTATCCGTT 
TCCCTGGCCA GCAGCTTCTT CGACTATTTC GATGAGGGCG TGCACCGGCT GTACAACTTT 
TACGGATCCA CGGAGGTATT GGGCGATGTC ACCTACTTTG CGTGCGAGAG CAAGAAGCAA 
CTGAGTTTGT ACGATAATGT GCCCATAGGC ATTCCACTGT CGAATACCGT GGTCTATTTG 
TTGGATGCCG ACTATCGTCC GGTGAAGAAC GGCGAGATTG GGGAGATCTT TGCTTCGGGT 
CTCAACTTGG CCGCTGGTTA TGTTAACGGA CGCGATCCGG AGAGGTTCTT GGAGAATCCT 
TTAGCTGTGG AGAAGAAATA CGCCCGTTTG TATCGCACTG GAGACTATGG ATCCCTGAAG 
AACGGCAGCA TCATGTACGA GGGACGCACC GACTCTCAGG TGAAGATCCG TGGACATCGC 
GTCGACCTGT CCGAAGTGGA GAAGAACGTG GCCGAGCTGC CGCTGGTGGA TAAGGCCATT 
GTTCTGTGCT ACCATGCTGG TCAGGTGGAT CAGGCTATTC TGGCCTTCGT CAAGCTGCGC 
GATGATGCCC CCATGGTCAC CGAGATGCAG ATGGAGGCTC GTCTCAAGGA CAAGCTAGCC 
GATTACATGA CGCCGCAGGT GGTCATCCTG GAGCACGTTC CATTGCTGGT CAACGGCAAG 
GTGGATCGCC AGGCGCTGCT CAAAACCTAT GAGACGGCCA ATAACAACGA AGGTGACTCC 
AGTATCGTTC TGGACTTTGA CTACTCGCAG GTTCCTGAGG ATCTAAAGCT AACGGCACGG 
GATCTCTTCG AGACAGTGGG CGGCGTGATT GGACGCTCCA CCAGAGCAAC CCTCGCTCCG 
CACAGTAACT TCTATGAGCT GGGCGGCAAC TCGTTGAATT CCATTTTCAC GGTGACGCTG 
CTGCGCGAGA AGGGCTACAA CATCGGCATT TCCGAATTCA TTGCGGCCAA GAATCTGGGC 
GAGATCATCG AGAAGATGGC GGCCAACCAT GATGCAGTGC AGCTAGAGGA GGAATCCTTG 
AATGCTTGTC CGCATCTGAA GATGGAGGCG GTGCCGCTAC GCCTGGAGCA CCGCCAGGAA 
GTCATCGACA TTATTGTGGC TAGCTTCTAT AACAAGGCCG ACCTGGAGCA GTGGCTCAAG 
CCCGGAGTTT TGAGAACCGA CTACAGCGAT ATACTGAACG ATATCTGGAA TGTGCTGGTG 
GAGCGGGACC TAAGTTTCGT GGTCTACGAT ACCAACACGG ATCGCATTAT TGGCACAGCG 
TTGAACTTTG ATGCGCGCAA CGAGCCCGAG GTGGACATCA AGTCCAAGCT ACTCATTGTC 
TTCGAGTTTC TGGAGTTTTG CGAGGGTCCC ATCAGGGATA ACTACCTGCC CAAGGGTCTC 
AACCAAATCC TGCACTCGTT CATGATGGGC ACCGCCGAGA AGTTGAATCC CCGCGAGAAC 
ATCGCCTGCA TGCACTTTAT GGAGCACGAG GTTCTGCGGG TGGCCCGCGA GAAGCAGTTC 
GCCGGCATCT TCACCACCAA CACCAGTCCG CTGACGCAGC AGCTGGCGGA TGTCTACCAC 
TATAAGACGC TTCTCAACTT CCAGGTGAAC GAGTACGTGC ACTCCGACGG CAGCCGTCCA 
TTCGGCGATG CACCGGACGA GCAGCGCGCT ATTGTCCATT GGAAGGAGGT GGGCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524431.2
CDS	300..2939
Translation	MGSLPQLSIVKGLQQDFVPRALHRIFEEQQLRHADKVALIYQPSTTGQGMAPSQSSYRQMNERANRAARLLVAETHGRFLQPNSDGDFIVAVCMQPSEGLVTTLLAIWKAGGAYLPIDPSFPANRIHHILLEAKPTLVIRDDDIDAGRFQGTPTLSTTELYAKSLQLAGSNLLSEEMLRGGNDHIAIVLYTSGSTGVPKGVRLPHESILNRLQWQWATFPYTANEAVSVFKTALTFVDSIAELWGPLMCGLAILVVPKAVTKDPQRLVALLERYKIRRLVLVPTLLRSLLMYLKMEGGGAAQKLLYNLQIWVCSGEPLSVSLASSFFDYFDEGVHRLYNFYGSTEVLGDVTYFACESKKQLSLYDNVPIGIPLSNTVVYLLDADYRPVKNGEIGEIFASGLNLAAGYVNGRDPERFLENPLAVEKKYARLYRTGDYGSLKNGSIMYEGRTDSQVKIRGHRVDLSEVEKNVAELPLVDKAIVLCYHAGQVDQAILAFVKLRDDAPMVTEMQMEARLKDKLADYMTPQVVILEHVPLLVNGKVDRQALLKTYETANNNEGDSSIVLDFDYSQVPEDLKLTARDLFETVGGVIGRSTRATLAPHSNFYELGGNSLNSIFTVTLLREKGYNIGISEFIAAKNLGEIIEKMAANHDAVQLEEESLNACPHLKMEAVPLRLEHRQEVIDIIVASFYNKADLEQWLKPGVLRTDYSDILNDIWNVLVERDLSFVVYDTNTDRIIGTALNFDARNEPEVDIKSKLLIVFEFLEFCEGPIRDNYLPKGLNQILHSFMMGTAEKLNPRENIACMHFMEHEVLRVAREKQFAGIFTTNTSPLTQQLADVYHYKTLLNFQVNEYVHSDGSRPFGDAPDEQRAIVHWKEVGK

Target ORF information:

RefSeq Version	NM_079707.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ebony (e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079707.4

ORF Insert Sequence:

ATGGGTTCGC TGCCACAATT GTCGATCGTC AAGGGTCTGC AGCAAGACTT CGTGCCTAGA 
GCTCTGCACC GCATCTTCGA GGAGCAGCAG CTGCGGCATG CCGACAAGGT GGCTCTGATC 
TATCAGCCCA GCACTACGGG CCAGGGAATG GCGCCCAGTC AGAGCAGCTA CCGCCAGATG 
AACGAGCGTG CGAACCGGGC AGCCCGCCTC CTGGTGGCCG AGACCCACGG CCGGTTCCTG 
CAGCCAAACA GCGATGGTGA CTTCATCGTG GCTGTGTGCA TGCAGCCGTC GGAGGGATTG 
GTCACCACAC TGCTGGCCAT CTGGAAGGCT GGCGGCGCGT ATTTGCCCAT CGATCCCAGC 
TTCCCGGCGA ACCGCATTCA CCACATACTG CTGGAGGCGA AGCCCACCTT GGTGATTCGC 
GACGATGACA TCGACGCCGG CCGTTTCCAG GGAACTCCCA CGTTATCCAC CACCGAACTG 
TATGCCAAAT CCCTCCAGCT GGCCGGCTCC AATCTGCTCT CAGAGGAGAT GCTGCGCGGT 
GGCAACGACC ACATCGCCAT CGTGCTCTAC ACCTCGGGCA GTACGGGTGT GCCCAAGGGA 
GTGCGTCTGC CGCACGAGAG CATCCTCAAT CGGCTCCAGT GGCAGTGGGC GACCTTTCCG 
TACACCGCCA ACGAGGCGGT GAGCGTTTTC AAGACGGCCC TAACCTTCGT GGACTCGATT 
GCGGAGCTAT GGGGCCCGTT AATGTGTGGC CTGGCCATTC TGGTGGTGCC CAAGGCAGTG 
ACCAAGGATC CCCAGCGATT GGTGGCCCTG TTGGAGCGTT ATAAGATCAG GCGTCTCGTA 
CTGGTGCCCA CCCTTCTGCG CTCGTTGCTC ATGTATCTGA AGATGGAGGG AGGCGGAGCT 
GCTCAGAAAC TGCTGTACAA TCTCCAGATT TGGGTCTGCT CCGGAGAACC CCTATCCGTT 
TCCCTGGCCA GCAGCTTCTT CGACTATTTC GATGAGGGCG TGCACCGGCT GTACAACTTT 
TACGGATCCA CGGAGGTATT GGGCGATGTC ACCTACTTTG CGTGCGAGAG CAAGAAGCAA 
CTGAGTTTGT ACGATAATGT GCCCATAGGC ATTCCACTGT CGAATACCGT GGTCTATTTG 
TTGGATGCCG ACTATCGTCC GGTGAAGAAC GGCGAGATTG GGGAGATCTT TGCTTCGGGT 
CTCAACTTGG CCGCTGGTTA TGTTAACGGA CGCGATCCGG AGAGGTTCTT GGAGAATCCT 
TTAGCTGTGG AGAAGAAATA CGCCCGTTTG TATCGCACTG GAGACTATGG ATCCCTGAAG 
AACGGCAGCA TCATGTACGA GGGACGCACC GACTCTCAGG TGAAGATCCG TGGACATCGC 
GTCGACCTGT CCGAAGTGGA GAAGAACGTG GCCGAGCTGC CGCTGGTGGA TAAGGCCATT 
GTTCTGTGCT ACCATGCTGG TCAGGTGGAT CAGGCTATTC TGGCCTTCGT CAAGCTGCGC 
GATGATGCCC CCATGGTCAC CGAGATGCAG ATGGAGGCTC GTCTCAAGGA CAAGCTAGCC 
GATTACATGA CGCCGCAGGT GGTCATCCTG GAGCACGTTC CATTGCTGGT CAACGGCAAG 
GTGGATCGCC AGGCGCTGCT CAAAACCTAT GAGACGGCCA ATAACAACGA AGGTGACTCC 
AGTATCGTTC TGGACTTTGA CTACTCGCAG GTTCCTGAGG ATCTAAAGCT AACGGCACGG 
GATCTCTTCG AGACAGTGGG CGGCGTGATT GGACGCTCCA CCAGAGCAAC CCTCGCTCCG 
CACAGTAACT TCTATGAGCT GGGCGGCAAC TCGTTGAATT CCATTTTCAC GGTGACGCTG 
CTGCGCGAGA AGGGCTACAA CATCGGCATT TCCGAATTCA TTGCGGCCAA GAATCTGGGC 
GAGATCATCG AGAAGATGGC GGCCAACCAT GATGCAGTGC AGCTAGAGGA GGAATCCTTG 
AATGCTTGTC CGCATCTGAA GATGGAGGCG GTGCCGCTAC GCCTGGAGCA CCGCCAGGAA 
GTCATCGACA TTATTGTGGC TAGCTTCTAT AACAAGGCCG ACCTGGAGCA GTGGCTCAAG 
CCCGGAGTTT TGAGAACCGA CTACAGCGAT ATACTGAACG ATATCTGGAA TGTGCTGGTG 
GAGCGGGACC TAAGTTTCGT GGTCTACGAT ACCAACACGG ATCGCATTAT TGGCACAGCG 
TTGAACTTTG ATGCGCGCAA CGAGCCCGAG GTGGACATCA AGTCCAAGCT ACTCATTGTC 
TTCGAGTTTC TGGAGTTTTG CGAGGGTCCC ATCAGGGATA ACTACCTGCC CAAGGGTCTC 
AACCAAATCC TGCACTCGTT CATGATGGGC ACCGCCGAGA AGTTGAATCC CCGCGAGAAC 
ATCGCCTGCA TGCACTTTAT GGAGCACGAG GTTCTGCGGG TGGCCCGCGA GAAGCAGTTC 
GCCGGCATCT TCACCACCAA CACCAGTCCG CTGACGCAGC AGCTGGCGGA TGTCTACCAC 
TATAAGACGC TTCTCAACTT CCAGGTGAAC GAGTACGTGC ACTCCGACGG CAGCCGTCCA 
TTCGGCGATG CACCGGACGA GCAGCGCGCT ATTGTCCATT GGAAGGAGGT GGGCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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e cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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