slmb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	slmb
Entrez Gene ID	42504
Full Name	supernumerary limbs
Synonyms	BcDNA:GM02031,CG3412,Dmel\CG3412,FBXW1,MENE (3R)-B,MENE(3R)-B,SLIMB,SLMB,Slimb,Slimb/Beta-TRCP1,Slimb/beta-TRCP,Slimb/betaTrCP,Slmb,crd,ica,l(3)00295,shv,slimb,wel
General protein information	Preferred Names supernumerary limbs Names CG3412 gene product from transcript CG3412-RA CG3412-PA CG3412-PB CG3412-PC SLIMB centrosome replication defective icare pingiel shiva slmb-PA slmb-PB slmb-PC wellman
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 93B13-93C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275872.1	NP_001262801.1	supernumerary limbs, isoform C
NM_001275873.1	NP_001262802.1	supernumerary limbs, isoform B
NM_079706.3	NP_524430.1	supernumerary limbs, isoform A

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	btrc	NP_001016386.1
Canis lupus familiaris (dog)	BTRC	XP_543980.3
Gallus gallus (chicken)	BTRC	XP_004942275.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001944	XP_321118.5
Homo sapiens (human)	BTRC	NP_378663.1
Pan troglodytes (chimpanzee)	BTRC	XP_507991.3
Bos taurus (cattle)	BTRC	NP_001076944.1
Mus musculus (house mouse)	Btrc	NP_001273394.1
Rattus norvegicus (Norway rat)	Btrc	NP_001007149.2
Drosophila melanogaster (fruit fly)	slmb	NP_001262801.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data revealed a new link to understand the underlying mechanism for Nerfin-1 turnover in post-translational level, and provided useful insights in animal development and disease treatment by manipulating the activity of Slimb and Nerfin-1.
Title: The SCF ubiquitin ligase Slimb controls Nerfin-1 turnover in Drosophila.

Interaction with Slimb leads to the ubiquitination and degradation of dSmurf, resulting in negative regulation of dSmurf-mediated Yki phosphorylation and activity in the Hippo pathway. Thus our study revealed a new regulatory mechanism of the Hippo pathway which may provide implications for developing tumor treatment.
Title: The Drosophila F-box protein Slimb controls dSmurf protein turnover to regulate the Hippo pathway.

When Asl reduction is attenuated by Asl overexpression, plk4 mutations, Plk4 RNAi, or Slimb overexpression, Asl levels are higher in spermatozoa, resulting in embryos with reduced viability.
Title: Asterless Reduction during Spermiogenesis Is Regulated by Plk4 and Is Essential for Zygote Development in Drosophila.

Slimb targets Bax and Debcl to the proteasome.
Title: The drosophila Bcl-2 family protein Debcl is targeted to the proteasome by the ?-TrCP homologue slimb.

Slmb has a role in control of oocyte and epithelial polarity by antagonizing the aPKC/Par-6 complex
Title: Slmb antagonises the aPKC/Par-6 complex to control oocyte and epithelial polarity.

The following slmb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slmb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05560	NM_001275872.1 Latest version!	Drosophila melanogaster supernumerary limbs (slmb), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05560	NM_079706.3 Latest version!	Drosophila melanogaster supernumerary limbs (slmb), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa08601	NM_001275873.1 Latest version!	Drosophila melanogaster supernumerary limbs (slmb), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slmb ( NM_001275872.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262801.1 Drosophila melanogaster supernumerary limbs (slmb), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05560
Clone ID Related Accession (Same CDS sequence)	NM_001275872.1 , NM_079706.3
Accession Version	NM_001275872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supernumerary limbs, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAAAA TGGAGACTGA CAAAATAATG GACGAAACCA ACTCCAATGC ACAGGCCTTC 
ACAACCACTA TGCTGTACGA CCCGGTGCGC AAGAAAGACT CATCGCCCAC CTACCAAACG 
GAGCGGGAAC TCTGCTTTCA GTACTTCACC CAGTGGAGCG AGTCGGGCCA GGTGGACTTT 
GTGGAGCACC TGCTGTCGCG CATGTGCCAC TATCAACACG GACAGATCAA TGCCTATCTC 
AAGCCGATGC TCCAGCGGGA CTTTATCACA TTGCTGCCAA TCAAGGGTCT GGATCACATC 
GCAGAAAACA TTTTGTCGTA CTTGGATGCC GAATCGCTCA AATCATCCGA GCTGGTCTGC 
AAGGAATGGC TGCGCGTCAT TTCCGAGGGC ATGCTCTGGA AGAAGCTCAT CGAACGCAAG 
GTGCGCACAG ATTCCTTGTG GCGCGGACTG GCCGAGCGGC GTAATTGGAT GCAGTACCTC 
TTCAAGCCAA GACCGGGCCA GACTCAACGG CCACACTCAT TCCATCGCGA GTTGTTCCCC 
AAGATAATGA ATGACATTGA CAGCATAGAG AACAACTGGC GGACTGGCCG CCACATGCTG 
CGCCGCATCA ACTGCCGGTC CGAGAACTCG AAGGGTGTCT ATTGTCTGCA GTACGATGAC 
GGCAAGATTG TCTCCGGACT GAGGGACAAC ACCATCAAGA TCTGGGATCG CACGGATTTG 
CAGTGCGTTA AGACCCTAAT GGGCCACACT GGATCGGTGC TGTGCCTGCA GTATGACGAC 
AAGGTGATCA TCAGTGGCTC CAGCGACTCC ACCGTCCGCG TGTGGGACGT CAATACCGGC 
GAGATGGTCA ACACCCTTAT CCACCACTGC GAGGCGGTGC TGCACTTGCG CTTTAACAAT 
GGCATGATGG TTACCTGCTC CAAGGATCGC TCCATCGCCG TCTGGGACAT GACCTCACCC 
AGCGAGATTA CGCTGCGGCG CGTCCTTGTC GGTCACCGTG CCGCCGTCAA TGTGGTGGAC 
TTTGATGAGA AGTACATCGT GTCCGCCAGC GGGGATCGCA CCATCAAGGT CTGGTCCACA 
TCTAGCTGTG AATTCGTGCG CACCTTGAAT GGCCACAAGC GTGGCATCGC CTGCCTACAG 
TACAGAGATC GCCTGGTGGT CAGCGGCAGC TCAGACAATT CCATAAGACT GTGGGACATT 
GAGTGCGGTG CCTGTTTACG TGTCCTGGAG GGCCATGAGG AATTGGTTCG CTGCATTCGT 
TTCGATACGA AACGAATCGT TAGCGGTGCC TACGATGGCA AGATCAAGGT TTGGGATTTG 
GTCGCCGCCC TGGATCCAAG AGCAGCCTCC AATACTCTCT GTCTGAATAC CCTTGTGGAA 
CATACTGGTC GCGTATTTCG TTTGCAATTC GATGAGTTCC AGATTGTTAG CAGCTCGCAT 
GATGATACAA TTTTGATTTG GGACTTTCTA AATTTCACAC CCAATGAGAA CAAGACCGGA 
CGCACACCGT CACCGGCCTT GATGGAACAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262801.1
CDS	492..2024
Translation	MMKMETDKIMDETNSNAQAFTTTMLYDPVRKKDSSPTYQTERELCFQYFTQWSESGQVDFVEHLLSRMCHYQHGQINAYLKPMLQRDFITLLPIKGLDHIAENILSYLDAESLKSSELVCKEWLRVISEGMLWKKLIERKVRTDSLWRGLAERRNWMQYLFKPRPGQTQRPHSFHRELFPKIMNDIDSIENNWRTGRHMLRRINCRSENSKGVYCLQYDDGKIVSGLRDNTIKIWDRTDLQCVKTLMGHTGSVLCLQYDDKVIISGSSDSTVRVWDVNTGEMVNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMTSPSEITLRRVLVGHRAAVNVVDFDEKYIVSASGDRTIKVWSTSSCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNSIRLWDIECGACLRVLEGHEELVRCIRFDTKRIVSGAYDGKIKVWDLVAALDPRAASNTLCLNTLVEHTGRVFRLQFDEFQIVSSSHDDTILIWDFLNFTPNENKTGRTPSPALMEH

Target ORF information:

RefSeq Version	NM_001275872.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supernumerary limbs (slmb), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275872.1

ORF Insert Sequence:

ATGATGAAAA TGGAGACTGA CAAAATAATG GACGAAACCA ACTCCAATGC ACAGGCCTTC 
ACAACCACTA TGCTGTACGA CCCGGTGCGC AAGAAAGACT CATCGCCCAC CTACCAAACG 
GAGCGGGAAC TCTGCTTTCA GTACTTCACC CAGTGGAGCG AGTCGGGCCA GGTGGACTTT 
GTGGAGCACC TGCTGTCGCG CATGTGCCAC TATCAACACG GACAGATCAA TGCCTATCTC 
AAGCCGATGC TCCAGCGGGA CTTTATCACA TTGCTGCCAA TCAAGGGTCT GGATCACATC 
GCAGAAAACA TTTTGTCGTA CTTGGATGCC GAATCGCTCA AATCATCCGA GCTGGTCTGC 
AAGGAATGGC TGCGCGTCAT TTCCGAGGGC ATGCTCTGGA AGAAGCTCAT CGAACGCAAG 
GTGCGCACAG ATTCCTTGTG GCGCGGACTG GCCGAGCGGC GTAATTGGAT GCAGTACCTC 
TTCAAGCCAA GACCGGGCCA GACTCAACGG CCACACTCAT TCCATCGCGA GTTGTTCCCC 
AAGATAATGA ATGACATTGA CAGCATAGAG AACAACTGGC GGACTGGCCG CCACATGCTG 
CGCCGCATCA ACTGCCGGTC CGAGAACTCG AAGGGTGTCT ATTGTCTGCA GTACGATGAC 
GGCAAGATTG TCTCCGGACT GAGGGACAAC ACCATCAAGA TCTGGGATCG CACGGATTTG 
CAGTGCGTTA AGACCCTAAT GGGCCACACT GGATCGGTGC TGTGCCTGCA GTATGACGAC 
AAGGTGATCA TCAGTGGCTC CAGCGACTCC ACCGTCCGCG TGTGGGACGT CAATACCGGC 
GAGATGGTCA ACACCCTTAT CCACCACTGC GAGGCGGTGC TGCACTTGCG CTTTAACAAT 
GGCATGATGG TTACCTGCTC CAAGGATCGC TCCATCGCCG TCTGGGACAT GACCTCACCC 
AGCGAGATTA CGCTGCGGCG CGTCCTTGTC GGTCACCGTG CCGCCGTCAA TGTGGTGGAC 
TTTGATGAGA AGTACATCGT GTCCGCCAGC GGGGATCGCA CCATCAAGGT CTGGTCCACA 
TCTAGCTGTG AATTCGTGCG CACCTTGAAT GGCCACAAGC GTGGCATCGC CTGCCTACAG 
TACAGAGATC GCCTGGTGGT CAGCGGCAGC TCAGACAATT CCATAAGACT GTGGGACATT 
GAGTGCGGTG CCTGTTTACG TGTCCTGGAG GGCCATGAGG AATTGGTTCG CTGCATTCGT 
TTCGATACGA AACGAATCGT TAGCGGTGCC TACGATGGCA AGATCAAGGT TTGGGATTTG 
GTCGCCGCCC TGGATCCAAG AGCAGCCTCC AATACTCTCT GTCTGAATAC CCTTGTGGAA 
CATACTGGTC GCGTATTTCG TTTGCAATTC GATGAGTTCC AGATTGTTAG CAGCTCGCAT 
GATGATACAA TTTTGATTTG GGACTTTCTA AATTTCACAC CCAATGAGAA CAAGACCGGA 
CGCACACCGT CACCGGCCTT GATGGAACAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slmb ( NM_079706.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524430.1 Drosophila melanogaster supernumerary limbs (slmb), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05560
Clone ID Related Accession (Same CDS sequence)	NM_001275872.1 , NM_079706.3
Accession Version	NM_079706.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supernumerary limbs, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079706.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAAAA TGGAGACTGA CAAAATAATG GACGAAACCA ACTCCAATGC ACAGGCCTTC 
ACAACCACTA TGCTGTACGA CCCGGTGCGC AAGAAAGACT CATCGCCCAC CTACCAAACG 
GAGCGGGAAC TCTGCTTTCA GTACTTCACC CAGTGGAGCG AGTCGGGCCA GGTGGACTTT 
GTGGAGCACC TGCTGTCGCG CATGTGCCAC TATCAACACG GACAGATCAA TGCCTATCTC 
AAGCCGATGC TCCAGCGGGA CTTTATCACA TTGCTGCCAA TCAAGGGTCT GGATCACATC 
GCAGAAAACA TTTTGTCGTA CTTGGATGCC GAATCGCTCA AATCATCCGA GCTGGTCTGC 
AAGGAATGGC TGCGCGTCAT TTCCGAGGGC ATGCTCTGGA AGAAGCTCAT CGAACGCAAG 
GTGCGCACAG ATTCCTTGTG GCGCGGACTG GCCGAGCGGC GTAATTGGAT GCAGTACCTC 
TTCAAGCCAA GACCGGGCCA GACTCAACGG CCACACTCAT TCCATCGCGA GTTGTTCCCC 
AAGATAATGA ATGACATTGA CAGCATAGAG AACAACTGGC GGACTGGCCG CCACATGCTG 
CGCCGCATCA ACTGCCGGTC CGAGAACTCG AAGGGTGTCT ATTGTCTGCA GTACGATGAC 
GGCAAGATTG TCTCCGGACT GAGGGACAAC ACCATCAAGA TCTGGGATCG CACGGATTTG 
CAGTGCGTTA AGACCCTAAT GGGCCACACT GGATCGGTGC TGTGCCTGCA GTATGACGAC 
AAGGTGATCA TCAGTGGCTC CAGCGACTCC ACCGTCCGCG TGTGGGACGT CAATACCGGC 
GAGATGGTCA ACACCCTTAT CCACCACTGC GAGGCGGTGC TGCACTTGCG CTTTAACAAT 
GGCATGATGG TTACCTGCTC CAAGGATCGC TCCATCGCCG TCTGGGACAT GACCTCACCC 
AGCGAGATTA CGCTGCGGCG CGTCCTTGTC GGTCACCGTG CCGCCGTCAA TGTGGTGGAC 
TTTGATGAGA AGTACATCGT GTCCGCCAGC GGGGATCGCA CCATCAAGGT CTGGTCCACA 
TCTAGCTGTG AATTCGTGCG CACCTTGAAT GGCCACAAGC GTGGCATCGC CTGCCTACAG 
TACAGAGATC GCCTGGTGGT CAGCGGCAGC TCAGACAATT CCATAAGACT GTGGGACATT 
GAGTGCGGTG CCTGTTTACG TGTCCTGGAG GGCCATGAGG AATTGGTTCG CTGCATTCGT 
TTCGATACGA AACGAATCGT TAGCGGTGCC TACGATGGCA AGATCAAGGT TTGGGATTTG 
GTCGCCGCCC TGGATCCAAG AGCAGCCTCC AATACTCTCT GTCTGAATAC CCTTGTGGAA 
CATACTGGTC GCGTATTTCG TTTGCAATTC GATGAGTTCC AGATTGTTAG CAGCTCGCAT 
GATGATACAA TTTTGATTTG GGACTTTCTA AATTTCACAC CCAATGAGAA CAAGACCGGA 
CGCACACCGT CACCGGCCTT GATGGAACAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524430.1
CDS	492..2024
Translation	MMKMETDKIMDETNSNAQAFTTTMLYDPVRKKDSSPTYQTERELCFQYFTQWSESGQVDFVEHLLSRMCHYQHGQINAYLKPMLQRDFITLLPIKGLDHIAENILSYLDAESLKSSELVCKEWLRVISEGMLWKKLIERKVRTDSLWRGLAERRNWMQYLFKPRPGQTQRPHSFHRELFPKIMNDIDSIENNWRTGRHMLRRINCRSENSKGVYCLQYDDGKIVSGLRDNTIKIWDRTDLQCVKTLMGHTGSVLCLQYDDKVIISGSSDSTVRVWDVNTGEMVNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMTSPSEITLRRVLVGHRAAVNVVDFDEKYIVSASGDRTIKVWSTSSCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNSIRLWDIECGACLRVLEGHEELVRCIRFDTKRIVSGAYDGKIKVWDLVAALDPRAASNTLCLNTLVEHTGRVFRLQFDEFQIVSSSHDDTILIWDFLNFTPNENKTGRTPSPALMEH

Target ORF information:

RefSeq Version	NM_079706.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supernumerary limbs (slmb), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079706.3

ORF Insert Sequence:

ATGATGAAAA TGGAGACTGA CAAAATAATG GACGAAACCA ACTCCAATGC ACAGGCCTTC 
ACAACCACTA TGCTGTACGA CCCGGTGCGC AAGAAAGACT CATCGCCCAC CTACCAAACG 
GAGCGGGAAC TCTGCTTTCA GTACTTCACC CAGTGGAGCG AGTCGGGCCA GGTGGACTTT 
GTGGAGCACC TGCTGTCGCG CATGTGCCAC TATCAACACG GACAGATCAA TGCCTATCTC 
AAGCCGATGC TCCAGCGGGA CTTTATCACA TTGCTGCCAA TCAAGGGTCT GGATCACATC 
GCAGAAAACA TTTTGTCGTA CTTGGATGCC GAATCGCTCA AATCATCCGA GCTGGTCTGC 
AAGGAATGGC TGCGCGTCAT TTCCGAGGGC ATGCTCTGGA AGAAGCTCAT CGAACGCAAG 
GTGCGCACAG ATTCCTTGTG GCGCGGACTG GCCGAGCGGC GTAATTGGAT GCAGTACCTC 
TTCAAGCCAA GACCGGGCCA GACTCAACGG CCACACTCAT TCCATCGCGA GTTGTTCCCC 
AAGATAATGA ATGACATTGA CAGCATAGAG AACAACTGGC GGACTGGCCG CCACATGCTG 
CGCCGCATCA ACTGCCGGTC CGAGAACTCG AAGGGTGTCT ATTGTCTGCA GTACGATGAC 
GGCAAGATTG TCTCCGGACT GAGGGACAAC ACCATCAAGA TCTGGGATCG CACGGATTTG 
CAGTGCGTTA AGACCCTAAT GGGCCACACT GGATCGGTGC TGTGCCTGCA GTATGACGAC 
AAGGTGATCA TCAGTGGCTC CAGCGACTCC ACCGTCCGCG TGTGGGACGT CAATACCGGC 
GAGATGGTCA ACACCCTTAT CCACCACTGC GAGGCGGTGC TGCACTTGCG CTTTAACAAT 
GGCATGATGG TTACCTGCTC CAAGGATCGC TCCATCGCCG TCTGGGACAT GACCTCACCC 
AGCGAGATTA CGCTGCGGCG CGTCCTTGTC GGTCACCGTG CCGCCGTCAA TGTGGTGGAC 
TTTGATGAGA AGTACATCGT GTCCGCCAGC GGGGATCGCA CCATCAAGGT CTGGTCCACA 
TCTAGCTGTG AATTCGTGCG CACCTTGAAT GGCCACAAGC GTGGCATCGC CTGCCTACAG 
TACAGAGATC GCCTGGTGGT CAGCGGCAGC TCAGACAATT CCATAAGACT GTGGGACATT 
GAGTGCGGTG CCTGTTTACG TGTCCTGGAG GGCCATGAGG AATTGGTTCG CTGCATTCGT 
TTCGATACGA AACGAATCGT TAGCGGTGCC TACGATGGCA AGATCAAGGT TTGGGATTTG 
GTCGCCGCCC TGGATCCAAG AGCAGCCTCC AATACTCTCT GTCTGAATAC CCTTGTGGAA 
CATACTGGTC GCGTATTTCG TTTGCAATTC GATGAGTTCC AGATTGTTAG CAGCTCGCAT 
GATGATACAA TTTTGATTTG GGACTTTCTA AATTTCACAC CCAATGAGAA CAAGACCGGA 
CGCACACCGT CACCGGCCTT GATGGAACAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slmb ( NM_001275873.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262802.1 Drosophila melanogaster supernumerary limbs (slmb), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08601
Clone ID Related Accession (Same CDS sequence)	NM_001275873.1
Accession Version	NM_001275873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supernumerary limbs, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAAAA TGGAGACTGA CAAAATAATG GACGAAACCA ACTCCAATGC ACAGGCCTTC 
ACAACCACTA TGCTGTACGA CCCGGTGCGC AAGAAAGACT CATCGCCCAC CTACCAAACG 
GAGCGGGAAC TCTGCTTTCA GTACTTCACC CAGTGGAGCG AGTCGGGCCA GGTGGACTTT 
GTGGAGCACC TGCTGTCGCG CATGTGCCAC TATCAACACG GACAGATCAA TGCCTATCTC 
AAGCCGATGC TCCAGCGGGA CTTTATCACA TTGCTGCCAA TCAAGGGTCT GGATCACATC 
GCAGAAAACA TTTTGTCGTA CTTGGATGCC GAATCGCTCA AATCATCCGA GCTGGTCTGC 
AAGGAATGGC TGCGCGTCAT TTCCGAGGGC ATGCTCTGGA AGAAGCTCAT CGAACGCAAG 
GTGCGCACAG ATTCCTTGTG GCGCGGACTG GCCGAGCGGC GTAATTGGAT GCAGTACCTC 
TTCAAGCCAA GACCGGGCCA GACTCAACGG CCACACTCAT TCCATCGCGA GTTGTTCCCC 
AAGATAATGA ATGACATTGA CAGCATAGAG AACAACTGGC GGACTGGCCG CCACATGCTG 
CGCCGCATCA ACTGCCGGTC CGAGAACTCG AAGGGTGTCT ATTGTCTGCA GTACGATGAC 
GGCAAGATTG TCTCCGGACT GAGGGACAAC ACCATCAAGA TCTGGGATCG CACGGATTTG 
CAGTGCGTTA AGACCCTAAT GGGCCACACT GGATCGGTGC TGTGCCTGCA GTATGACGAC 
AAGGTGATCA TCAGTGGCTC CAGCGACTCC ACCGTCCGCG TGTGGGACGT CAATACCGGC 
GAGATGGTCA ACACCCTTAT CCACCACTGC GAGGCGGTGC TGCACTTGCG CTTTAACAAT 
GGCATGATGG TTACCTGCTC CAAGGATCGC TCCATCGCCG TCTGGGACAT GACCTCACCC 
AGCGAGATTA CGCTGCGGCG CGTCCTTGTC GGTCACCGTG CCGCCGTCAA TGTGGTGGAC 
TTTGATGAGA AGTACATCGT GTCCGCCAGC GGGGATCGCA CCATCAAGGT CTGGTCCACA 
TCTAGCTGTG AATTCGTGCG CACCTTGAAT GGCCACAAGC GTGGCATCGC CTGCCTACAG 
TACAGAGATC GCCTGGTGGT CAGCGGCAGC TCAGACAATT CCATAAGACT GTGGGACATT 
GAGTGCGGTG CCTGTTTACG TGTCCTGGAG GGCCATGAGG AATTGGTTCG CTGCATTCGT 
TTCGATACGA AACGAATCGT TAGCGGTGCC TACGATGGCA AGATCAAGGT TTGGGATTTG 
GTCGCCGCCC TGGATCCAAG AGCAGCCTCC AATACTCTCT GTCTGAATAC CCTTGTGGAA 
CATACTGGTC GCGTATTTCG TTTGCAATTC GATGAGTTCC AGATTGTTAG CAGCTCGCAT 
GATGATACAA TTTTGATTTG GGACTTTCTA AATTTCACAC CCAATGAGAA CAAGACCGGA 
CGCACACCGT CACCGGCCTT GATGGAACAT TAACATTTTG TGCAACGGCA GCTGCGCGAG 
TTACATCTAC AATCCTCGAG CGAAGGAGAG GAGGACGACG ATGCGGACGA GGACGACGAG 
GATGATTTTG AGGAGGAGGA TTCTAGAAAC GATGCTGGCG CGTTTATGGG GGGATATGTT 
AGGCACCGGG ATGCTGGTGG TTCGGGATCC GTCACCGGTT CCGGTTCGGA TCCCGAATCG 
GATGACCTGG ATTTCGATAT AGATGTTGAG AATATTAATG ATTATGATGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262802.1
CDS	492..2285
Translation	MMKMETDKIMDETNSNAQAFTTTMLYDPVRKKDSSPTYQTERELCFQYFTQWSESGQVDFVEHLLSRMCHYQHGQINAYLKPMLQRDFITLLPIKGLDHIAENILSYLDAESLKSSELVCKEWLRVISEGMLWKKLIERKVRTDSLWRGLAERRNWMQYLFKPRPGQTQRPHSFHRELFPKIMNDIDSIENNWRTGRHMLRRINCRSENSKGVYCLQYDDGKIVSGLRDNTIKIWDRTDLQCVKTLMGHTGSVLCLQYDDKVIISGSSDSTVRVWDVNTGEMVNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMTSPSEITLRRVLVGHRAAVNVVDFDEKYIVSASGDRTIKVWSTSSCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNSIRLWDIECGACLRVLEGHEELVRCIRFDTKRIVSGAYDGKIKVWDLVAALDPRAASNTLCLNTLVEHTGRVFRLQFDEFQIVSSSHDDTILIWDFLNFTPNENKTGRTPSPALMEH*HFVQRQLRELHLQSSSEGEEDDDADEDDEDDFEEEDSRNDAGAFMGGYVRHRDAGGSGSVTGSGSDPESDDLDFDIDVENINDYDE

Target ORF information:

RefSeq Version	NM_001275873.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supernumerary limbs (slmb), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275873.1

ORF Insert Sequence:

ATGATGAAAA TGGAGACTGA CAAAATAATG GACGAAACCA ACTCCAATGC ACAGGCCTTC 
ACAACCACTA TGCTGTACGA CCCGGTGCGC AAGAAAGACT CATCGCCCAC CTACCAAACG 
GAGCGGGAAC TCTGCTTTCA GTACTTCACC CAGTGGAGCG AGTCGGGCCA GGTGGACTTT 
GTGGAGCACC TGCTGTCGCG CATGTGCCAC TATCAACACG GACAGATCAA TGCCTATCTC 
AAGCCGATGC TCCAGCGGGA CTTTATCACA TTGCTGCCAA TCAAGGGTCT GGATCACATC 
GCAGAAAACA TTTTGTCGTA CTTGGATGCC GAATCGCTCA AATCATCCGA GCTGGTCTGC 
AAGGAATGGC TGCGCGTCAT TTCCGAGGGC ATGCTCTGGA AGAAGCTCAT CGAACGCAAG 
GTGCGCACAG ATTCCTTGTG GCGCGGACTG GCCGAGCGGC GTAATTGGAT GCAGTACCTC 
TTCAAGCCAA GACCGGGCCA GACTCAACGG CCACACTCAT TCCATCGCGA GTTGTTCCCC 
AAGATAATGA ATGACATTGA CAGCATAGAG AACAACTGGC GGACTGGCCG CCACATGCTG 
CGCCGCATCA ACTGCCGGTC CGAGAACTCG AAGGGTGTCT ATTGTCTGCA GTACGATGAC 
GGCAAGATTG TCTCCGGACT GAGGGACAAC ACCATCAAGA TCTGGGATCG CACGGATTTG 
CAGTGCGTTA AGACCCTAAT GGGCCACACT GGATCGGTGC TGTGCCTGCA GTATGACGAC 
AAGGTGATCA TCAGTGGCTC CAGCGACTCC ACCGTCCGCG TGTGGGACGT CAATACCGGC 
GAGATGGTCA ACACCCTTAT CCACCACTGC GAGGCGGTGC TGCACTTGCG CTTTAACAAT 
GGCATGATGG TTACCTGCTC CAAGGATCGC TCCATCGCCG TCTGGGACAT GACCTCACCC 
AGCGAGATTA CGCTGCGGCG CGTCCTTGTC GGTCACCGTG CCGCCGTCAA TGTGGTGGAC 
TTTGATGAGA AGTACATCGT GTCCGCCAGC GGGGATCGCA CCATCAAGGT CTGGTCCACA 
TCTAGCTGTG AATTCGTGCG CACCTTGAAT GGCCACAAGC GTGGCATCGC CTGCCTACAG 
TACAGAGATC GCCTGGTGGT CAGCGGCAGC TCAGACAATT CCATAAGACT GTGGGACATT 
GAGTGCGGTG CCTGTTTACG TGTCCTGGAG GGCCATGAGG AATTGGTTCG CTGCATTCGT 
TTCGATACGA AACGAATCGT TAGCGGTGCC TACGATGGCA AGATCAAGGT TTGGGATTTG 
GTCGCCGCCC TGGATCCAAG AGCAGCCTCC AATACTCTCT GTCTGAATAC CCTTGTGGAA 
CATACTGGTC GCGTATTTCG TTTGCAATTC GATGAGTTCC AGATTGTTAG CAGCTCGCAT 
GATGATACAA TTTTGATTTG GGACTTTCTA AATTTCACAC CCAATGAGAA CAAGACCGGA 
CGCACACCGT CACCGGCCTT GATGGAACAT TAACATTTTG TGCAACGGCA GCTGCGCGAG 
TTACATCTAC AATCCTCGAG CGAAGGAGAG GAGGACGACG ATGCGGACGA GGACGACGAG 
GATGATTTTG AGGAGGAGGA TTCTAGAAAC GATGCTGGCG CGTTTATGGG GGGATATGTT 
AGGCACCGGG ATGCTGGTGG TTCGGGATCC GTCACCGGTT CCGGTTCGGA TCCCGAATCG 
GATGACCTGG ATTTCGATAT AGATGTTGAG AATATTAATG ATTATGATGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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slmb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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