Sec15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sec15
Entrez Gene ID	42499
Full Name	Secretory 15
Synonyms	3R41,CG7034,Dmel\CG7034,Sec15p,dsec15,sec15
General protein information	Preferred Names Secretory 15 Names CG7034 gene product from transcript CG7034-RA CG7034-PA Sec15 ortholog Sec15-PA sec15 secretory 15
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 93B12-93B12

mRNA and Protein(s)

mRNA	Protein	Name
NM_142685.3	NP_650942.1	secretory 15

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Exoc6b	NP_001102716.1
Schizosaccharomyces pombe (fission yeast)	sec15	NP_587884.2
Neurospora crassa	NCU00117	XP_957241.1
Oryza sativa (rice)	Os10g0414950	NP_001176150.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006648	XP_316678.1
Caenorhabditis elegans (roundworm)	sec-15	NP_509496.3
Eremothecium gossypii	AGOS_AFR251C	NP_985798.2
Pan troglodytes (chimpanzee)	EXOC6B	XP_515544.3
Canis lupus familiaris (dog)	EXOC6B	XP_540235.2
Drosophila melanogaster (fruit fly)	sec15	NP_650942.1
Saccharomyces cerevisiae (baker's yeast)	SEC15	NP_011281.1
Xenopus tropicalis (tropical clawed frog)	exoc6b	XP_002938448.2
Homo sapiens (human)	EXOC6B	NP_056004.1
Kluyveromyces lactis	KLLA0A01023g	XP_451044.1
Arabidopsis thaliana (thale cress)	SEC15A	NP_191223.2
Oryza sativa (rice)	Os10g0415200	NP_001064599.1
Mus musculus (house mouse)	Exoc6b	NP_796051.2
Gallus gallus (chicken)	EXOC6B	XP_004936350.1
Danio rerio (zebrafish)	exoc6b	NP_001070198.2
Magnaporthe oryzae (rice blast fungus)	MGG_00471	XP_003718593.1
Oryza sativa (rice)	Os03g0163500	NP_001049059.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In fly photoreceptors Sec15 colocalizes with Rab11 and that loss of Sec15 affects rhabdomere morphology.
Title: Sec15 interacts with Rab11 via a novel domain and affects Rab11 localization in vivo.

The Sec15 protein, a known Rab11 effector and a component of the exocyst, is necessary for Notch activation and the resolution of distinct sibling cell fates in the sensory organ precursors lineage.
Title: Drosophila exocyst components Sec5, Sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane.

The Sec15 protein, a known Rab11 effector and a component of the exocyst, is necessary for Notch activation and the resolution of distinct sibling cell fates in the sensory organ precursors lineage.
Title: Recycling polarity.

The Sec15 protein, a known Rab11 effector and a component of the exocyst, is necessary for Notch activation and the resolution of distinct sibling cell fates in the sensory organ precursors lineage.
Title: Sec15, a component of the exocyst, promotes notch signaling during the asymmetric division of Drosophila sensory organ precursors.

The following Sec15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sec15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05555	NM_142685.3 Latest version!	Drosophila melanogaster secretory 15 (Sec15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sec15 ( NM_142685.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650942.1 Drosophila melanogaster secretory 15 (Sec15), mRNA.

Price & Availability↑

CloneID	OFa05555
Clone ID Related Accession (Same CDS sequence)	NM_142685.3
Accession Version	NM_142685.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 15
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142685.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAAG TGACCGTGCA AGACATTGAG GCGGTGGACG ACTACTGGGG ACCAACATTC 
CGTTCGATCC TTGAAGGCAA CAACACTAAG CAGATTGGCG ACCAACTGGA GCAGCGGATA 
CGGAGCCATG ACAAAGAGAT CGAGCGGATC TGCAATCTGT ACTACCAGGG CTTCATCGAT 
TCAATTCAGG AGCTGCTCCA GGTGCGCACG CAGGCCCAAC AGCTGCACAA CGAAGTGCAT 
TCCTTGGACA CGTCCCTTCG CCAGATCAGC GCCTCGCTGA TTCAGCAGGG CAATGATCTA 
GTGCGGGCCC GTCAGATCGA ATCCAATCTG GCCAGCGCCA TCGAGGCACT CAAATCCTGT 
CTGCCCGCAT TGGAGTGCTA CATGAAGTTC ACACAGCAGG CAAAGAACAA GCAGTACTAC 
CAGGCGCTGA GAACTCTGGA GACTCTGGAG ACGGAGCACC TAACGCGCTT GAAGACACAC 
AACTACCGAT TCGCCACCCA GATGCAGATA CAGATTCCCA TAATCAAGGA AAACATCCGT 
CGCTCTTCCG CCTCGGACTT TCGAGAGTTC CTCGAGAACA TCCGGAAGTT TTCACCCCGC 
ATCGGCGAGC TGGCCATCAC ACACACAAAA CAGCTCCAAA AGCGTGATAT CAACGCCATC 
ATTGCCGAGC ACATGCAGCA GATGAATGGC GGCGAAGCCG GCGGAGCAGG TGCTGGCGGG 
GATGATGATG GTGCCAACGT TAGTGCCCAG GATCTCATTG ACTTCTCCCC CATCTACCGC 
TGCCTACACA TCTACATGGT GCTGGGTCAG CGGGAGTACT TCGAGAAGGA CTATCGTCAG 
CAGCGCAGGG ATCAGGCCAA ACTGGTGCTT CAACCGCCGC CCAACATGCA CGACAATTTG 
GAGGCCTACA AAACGTACAT CTGTGCCATC GTTGGCTTCT TCGTGGTTGA GGATCACGTT 
AAAAACACGG CGGGCGATGT CGTGACCAGC AGTTATTTGG AGGATCTGTG GTCCAGCTCG 
TTAACCAAGT TTGTCAACGA AATCAGCATG AGTTCTTCGT CCTGCACCGA TCCTAATATC 
CTTCTGCGCA TTAAGAACCT CATTATGCTG TCCATCAACA CCTTTAAGTG CTACGGTTAC 
ACCGTGAACA TCCTCTGGGA ACTATTGCAC AACATGCGGG ATCATTATAA TGAGGTTTTA 
CTGCAACGCT GGGTACATGT GTTCCGCGAA ATTCTCGACA AGGAACAGTT CCTGCCCATG 
GTTGTACAAA ACACGGAAGA GTATGAATGC ATCATCGAAC GATTCCCCTT CCACTCGGAA 
CAGCTTGAAA ATGCTCCGTT TCCGAAGAAA TTCCCCTTTT CGAGAATGGT GCCGGAGGTT 
TATCACCAGG CCAAGGAATT CATGTACGCC TGCATGAAGT TCGCCGAGGA GCTCACCCTA 
TCGCCCAACG AGGTGGCCGC CATGGTGCGC AAGGCGGCCA ATCTGCTGCT CACACGAAGT 
TTCAGTGGCT GTCTATCTGT GGTGTTCCGC CAGCCGAGCA TCACGCTGAC GCAGCTCATT 
CAGATCATAA TCGACACGCA GTACTTGGAG AAGGCAGGGC CGTTTCTGGA CGAGTTCGTG 
TGTCACATGA CCAACACGGA GAGGAGCGTA TCTCAGACAC CATCGGCCAT GTTCCACGTG 
GCCCGGCAAG ATGCCGAGAA GCAGGTGGGC CTACGTATTT GCTCGAAGAT CGATGAGTTC 
TTCGAACTAA GCGCCTATGA CTGGTTACTA GTAGAGCCGC CGGGAATAGC CTCCGCCTTC 
ATTACGGACA TGATTTCCTA CCTTAAGAGC ACCTTTGACA GCTTTGCCTT CAAGCTGCCC 
CACATCGCCC AGGCGGCCTG CCGACGCACC TTCGAGCACA TTGCAGAGAA GATTTACTCG 
ATCATGTATG ACGAGGATGT GAAGCAGATT TCCACGGGAG CACTCACACA AATCAACTTG 
GATCTGATGC AGTGCGAGTT TTTTGCGGCC TCGGAACCTG TGCCTGGTCT AAAGGAAGGA 
GAGCTGAGCA AGTACTTCCT ACGTAACCGT CAGCTGTTGG ACCTGCTCAT TCTAGAGGAA 
TGGAGCACGT ATTTCCATGA CTATGGCAAG CAAGAGAACC GCTACCATCT GGTGCAACCG 
CAGTCCATTA TTGTGATACT GGAAAAGATC CGCGAGGCGG ACAAAAAGCC AATTTTCTCG 
TTGGTGCGAA AGAATGACAA GAAGAAGCTT TTGGAGACGG TGCTCAAGCA GCTTAAGCAC 
ATTGCCGATA GACAGAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650942.1
CDS	80..2380
Translation	MSKVTVQDIEAVDDYWGPTFRSILEGNNTKQIGDQLEQRIRSHDKEIERICNLYYQGFIDSIQELLQVRTQAQQLHNEVHSLDTSLRQISASLIQQGNDLVRARQIESNLASAIEALKSCLPALECYMKFTQQAKNKQYYQALRTLETLETEHLTRLKTHNYRFATQMQIQIPIIKENIRRSSASDFREFLENIRKFSPRIGELAITHTKQLQKRDINAIIAEHMQQMNGGEAGGAGAGGDDDGANVSAQDLIDFSPIYRCLHIYMVLGQREYFEKDYRQQRRDQAKLVLQPPPNMHDNLEAYKTYICAIVGFFVVEDHVKNTAGDVVTSSYLEDLWSSSLTKFVNEISMSSSSCTDPNILLRIKNLIMLSINTFKCYGYTVNILWELLHNMRDHYNEVLLQRWVHVFREILDKEQFLPMVVQNTEEYECIIERFPFHSEQLENAPFPKKFPFSRMVPEVYHQAKEFMYACMKFAEELTLSPNEVAAMVRKAANLLLTRSFSGCLSVVFRQPSITLTQLIQIIIDTQYLEKAGPFLDEFVCHMTNTERSVSQTPSAMFHVARQDAEKQVGLRICSKIDEFFELSAYDWLLVEPPGIASAFITDMISYLKSTFDSFAFKLPHIAQAACRRTFEHIAEKIYSIMYDEDVKQISTGALTQINLDLMQCEFFAASEPVPGLKEGELSKYFLRNRQLLDLLILEEWSTYFHDYGKQENRYHLVQPQSIIVILEKIREADKKPIFSLVRKNDKKKLLETVLKQLKHIADRQN

Target ORF information:

RefSeq Version	NM_142685.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 15 (Sec15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142685.3

ORF Insert Sequence:

ATGTCCAAAG TGACCGTGCA AGACATTGAG GCGGTGGACG ACTACTGGGG ACCAACATTC 
CGTTCGATCC TTGAAGGCAA CAACACTAAG CAGATTGGCG ACCAACTGGA GCAGCGGATA 
CGGAGCCATG ACAAAGAGAT CGAGCGGATC TGCAATCTGT ACTACCAGGG CTTCATCGAT 
TCAATTCAGG AGCTGCTCCA GGTGCGCACG CAGGCCCAAC AGCTGCACAA CGAAGTGCAT 
TCCTTGGACA CGTCCCTTCG CCAGATCAGC GCCTCGCTGA TTCAGCAGGG CAATGATCTA 
GTGCGGGCCC GTCAGATCGA ATCCAATCTG GCCAGCGCCA TCGAGGCACT CAAATCCTGT 
CTGCCCGCAT TGGAGTGCTA CATGAAGTTC ACACAGCAGG CAAAGAACAA GCAGTACTAC 
CAGGCGCTGA GAACTCTGGA GACTCTGGAG ACGGAGCACC TAACGCGCTT GAAGACACAC 
AACTACCGAT TCGCCACCCA GATGCAGATA CAGATTCCCA TAATCAAGGA AAACATCCGT 
CGCTCTTCCG CCTCGGACTT TCGAGAGTTC CTCGAGAACA TCCGGAAGTT TTCACCCCGC 
ATCGGCGAGC TGGCCATCAC ACACACAAAA CAGCTCCAAA AGCGTGATAT CAACGCCATC 
ATTGCCGAGC ACATGCAGCA GATGAATGGC GGCGAAGCCG GCGGAGCAGG TGCTGGCGGG 
GATGATGATG GTGCCAACGT TAGTGCCCAG GATCTCATTG ACTTCTCCCC CATCTACCGC 
TGCCTACACA TCTACATGGT GCTGGGTCAG CGGGAGTACT TCGAGAAGGA CTATCGTCAG 
CAGCGCAGGG ATCAGGCCAA ACTGGTGCTT CAACCGCCGC CCAACATGCA CGACAATTTG 
GAGGCCTACA AAACGTACAT CTGTGCCATC GTTGGCTTCT TCGTGGTTGA GGATCACGTT 
AAAAACACGG CGGGCGATGT CGTGACCAGC AGTTATTTGG AGGATCTGTG GTCCAGCTCG 
TTAACCAAGT TTGTCAACGA AATCAGCATG AGTTCTTCGT CCTGCACCGA TCCTAATATC 
CTTCTGCGCA TTAAGAACCT CATTATGCTG TCCATCAACA CCTTTAAGTG CTACGGTTAC 
ACCGTGAACA TCCTCTGGGA ACTATTGCAC AACATGCGGG ATCATTATAA TGAGGTTTTA 
CTGCAACGCT GGGTACATGT GTTCCGCGAA ATTCTCGACA AGGAACAGTT CCTGCCCATG 
GTTGTACAAA ACACGGAAGA GTATGAATGC ATCATCGAAC GATTCCCCTT CCACTCGGAA 
CAGCTTGAAA ATGCTCCGTT TCCGAAGAAA TTCCCCTTTT CGAGAATGGT GCCGGAGGTT 
TATCACCAGG CCAAGGAATT CATGTACGCC TGCATGAAGT TCGCCGAGGA GCTCACCCTA 
TCGCCCAACG AGGTGGCCGC CATGGTGCGC AAGGCGGCCA ATCTGCTGCT CACACGAAGT 
TTCAGTGGCT GTCTATCTGT GGTGTTCCGC CAGCCGAGCA TCACGCTGAC GCAGCTCATT 
CAGATCATAA TCGACACGCA GTACTTGGAG AAGGCAGGGC CGTTTCTGGA CGAGTTCGTG 
TGTCACATGA CCAACACGGA GAGGAGCGTA TCTCAGACAC CATCGGCCAT GTTCCACGTG 
GCCCGGCAAG ATGCCGAGAA GCAGGTGGGC CTACGTATTT GCTCGAAGAT CGATGAGTTC 
TTCGAACTAA GCGCCTATGA CTGGTTACTA GTAGAGCCGC CGGGAATAGC CTCCGCCTTC 
ATTACGGACA TGATTTCCTA CCTTAAGAGC ACCTTTGACA GCTTTGCCTT CAAGCTGCCC 
CACATCGCCC AGGCGGCCTG CCGACGCACC TTCGAGCACA TTGCAGAGAA GATTTACTCG 
ATCATGTATG ACGAGGATGT GAAGCAGATT TCCACGGGAG CACTCACACA AATCAACTTG 
GATCTGATGC AGTGCGAGTT TTTTGCGGCC TCGGAACCTG TGCCTGGTCT AAAGGAAGGA 
GAGCTGAGCA AGTACTTCCT ACGTAACCGT CAGCTGTTGG ACCTGCTCAT TCTAGAGGAA 
TGGAGCACGT ATTTCCATGA CTATGGCAAG CAAGAGAACC GCTACCATCT GGTGCAACCG 
CAGTCCATTA TTGTGATACT GGAAAAGATC CGCGAGGCGG ACAAAAAGCC AATTTTCTCG 
TTGGTGCGAA AGAATGACAA GAAGAAGCTT TTGGAGACGG TGCTCAAGCA GCTTAAGCAC 
ATTGCCGATA GACAGAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sec15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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