Pi3K92E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pi3K92E
Entrez Gene ID	42446
Full Name	CG4141 gene product from transcript CG4141-RB
Synonyms	CG4141,DP110,Dmel\CG4141,Dmp110,Dp110,Dp110/PI3K,Dpp110,PI(3)K,PI-3 kinase,PI-3-K,PI3'K,PI3K,PI3K-92D,PI3K-92E/Dp110,PI3K-Dp110,PI3K-I,PI3K-dp110,PI3K92E,PI3k,PI[[3]]K,Pi3K,Pi3K92D,Pi3K92E,p110,Pi3Kp110,Pi3k,Pi3k92e,PiK92E,anon-92Ed,dP110,dP13K,dPI3K,dPI3K[92E],dp110,dp110alpha,p110,p120,pi3k,pi3k92e,rea,type-1 PI3K
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92F3-92F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_169910.2	NP_732536.1	Pi3K92E, isoform B
NM_001275841.1	NP_001262770.1	Pi3K92E, isoform C
NM_142645.3	NP_650902.1	Pi3K92E, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PIK3CD	XP_005620563.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000018	XP_311141.5
Pan troglodytes (chimpanzee)	PIK3CD	XP_001160824.1
Mus musculus (house mouse)	Pik3cd	NP_032866.2
Rattus norvegicus (Norway rat)	Pik3cd	NP_001102448.1
Danio rerio (zebrafish)	pik3cd	NP_957493.1
Homo sapiens (human)	PIK3CD	NP_005017.3
Bos taurus (cattle)	PIK3CD	NP_001192477.1
Gallus gallus (chicken)	PIK3CD	NP_001012714.1
Drosophila melanogaster (fruit fly)	Pi3K92E	NP_732536.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results indicate a novel kinase-independent function of the PI3K catalytic subunit.
Title: The Unique Non-Catalytic C-Terminus of P37delta-PI3K Adds Proliferative Properties In Vitro and In Vivo.

class I PI3K and PI(3,4,5)P(3) may be involved in the regulation of invagination during gastrulation
Title: Expression pattern of class I phosphoinositide 3-kinase and distribution of its product, phosphatidylinositol-3,4,5-trisphosphate, during Drosophila embryogenesis.

p53-related protein kinase is required for PI3K/TOR pathway-dependent growth.
Title: Drosophila p53-related protein kinase is required for PI3K/TOR pathway-dependent growth.

Hdac3 served as an important regulator of the PI3K pathway and revealed a novel link between histone acetylation and growth control.
Title: Depletion of histone deacetylase 3 antagonizes PI3K-mediated overgrowth of Drosophila organs through the acetylation of histone H4 at lysine 16.

PI3K requires Ras activity to decrease motor neuron excitability whereas the ability of PI3K to increase nerve terminal growth is Ras-independent.
Title: Ras-dependent and Ras-independent effects of PI3K in?Drosophila motor neurons.

The following Pi3K92E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pi3K92E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05496	NM_169910.2 Latest version!	Drosophila melanogaster Pi3K92E, transcript variant B (Pi3K92E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa05496	NM_142645.3 Latest version!	Drosophila melanogaster Pi3K92E, transcript variant A (Pi3K92E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa05496	NM_001275841.1 Latest version!	Drosophila melanogaster Pi3K92E, transcript variant C (Pi3K92E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pi3K92E ( NM_169910.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732536.1 Drosophila melanogaster Pi3K92E, transcript variant B (Pi3K92E), mRNA.

Price & Availability↑

CloneID	OFa05496
Clone ID Related Accession (Same CDS sequence)	NM_169910.2 , NM_001275841.1 , NM_142645.3
Accession Version	NM_169910.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Pi3K92E, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169910.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACATGA TGGACAACCG GGCGTTGGCC TACGTGGCCC ACCAGCCCAA GTATGAGACA 
CCGCCGGAAG AAGCGGAGCC GCCCTGCATG CGCTTTTCGG TTAACCTGTG GAAAAACGAG 
ATGCTGAACT GGGTGGACCT AATCTGCCTG TTGCCCAATG GATTCCTGCT GGAGCTCAGG 
GTCAATCCGG CCAACACCAT CCAGGTAATC AAGGTGGAGA TGGTCAACCA GGCCAAACAG 
ATGCCACTGG GCTATGTGAT CAAAGAGGCC TGCGAGTACC AGGTGTACGG CATCTCGACC 
TTCAACATCG AACCTTACAC CGACGAAACG AAGCGACTCA GTGAGGTCCA GCCGTACTTC 
GGCATCCTCA GCCTCGGCGA GCGCACCGAC ACAACGAGCT TTAGCAGCGA CTACGAGCTG 
ACCAAGATGG TTAACGGAAT GATCGGCACC ACCTTCGATC ATAATCGAAC GCACGGCTCG 
CCCGAGATTG ACGACTTCCG GCTGTATATG ACCCAAACTT GCGACAACAT CGAACTGGAG 
CGCTCCGCCT ACACCTGGCA GCAGAGACTG CTCTACGAGC ATCCTCTGCG ACTGGCGAAC 
TCGACTAAAA TGCCCGAGCT GATACGGGAG CGGCATCCGA CCAGAACCTT TCTCATCGTC 
GTAAAGAACG AGAACGACCA GAGCACGTTT ACGTTGTCGG TGAACGAGCA GGACACTCCC 
TTCTCGCTCA CGGAAAGCAC GCTGCAGAAG ATGAATCGGT CGCAGATGAA AATGAACGAC 
CGGACCTCCG ACTACATACT CAAAGTGAGC GGTCGCGACG AGTACCTGCT TGGCGACTAT 
CCCCTGATTC AGTTCCTGTA CATCCAAGAG ATGCTCTCGG ACTCGGCGGT TCCAAATGTG 
GTGTTGCAAT CAGTTTACCG CCTAGAGTCC TACATCAACC ACCACAACGA GCAGGCCATG 
GTAACCAAAC GACCGCTGCC GAAGAAACGG ACAGTCCACC TCCACAAGTC GATCTCCTCG 
CTGTGGGACA TGGGCAACTA CTTTCAGTTG ACGCTGCACA GCATTTCCAA TGTGAACTTT 
GACAAGACGC GTGCCCTCAA GGTGGGCGTG CATGTGTGCC TATATCACGG CGACAAGAAG 
TTATGCGCCC AGCGATCAAC GGACTCCCCT AACGGCAACT TTGACACGTT CCTGTTTAAT 
GACCTGGTGA TGGACTTTGA CATACAGATG CGCAACCTAC CGCGGATGAC ACGCCTCTGC 
ATTGTGATCT TCGAGGTGAC CAAGATGTCG CGCTCGAAGA AATCGTCCAA CAATAAGGAC 
ATCGCTCTGA AAGACGTCCC GTACAACAAA AACCCGCTGG CCTGGGTCAA CACAACAATT 
TTCGACCACA AGGACATCCT GCGCACCGGT CGCCATACGC TGTACACGTG GACGTATGCT 
GACGACATTC AGTCGGTTGA GGTTTTCCAT CCGCTGGGCA CCATCGAACC CAATCCGCGC 
AAAGAGGAAT GTGCGCTGGT GGACCTCACA TTCCTCAGCA GCGGAACCGG AACGGTGCGG 
TATCCCAGCG AGGAGGTCGT GTTGCAGTAC GCTGCGGATC GAGAGCAGGT CAATCGACTG 
CAACGGCAAC TGGCAGGGCC AGAAAAGCCG ATCAAAGAAC TGAAGGAGCT TATGGCCAAT 
TATACAGGAC TGGATAAGAT CTATGAGATG GTTGACCAGG ACCGCAATGC CATTTGGGAA 
CGCAGAAACG ACATTTTGCG TGAGCTTCCG GAAGAGCTGT CCATCTTACT TCACTGCGTC 
TATTGGAAGG AGCGTGATGA CGTAGCGGAT ATGTGGTATC TTCTCAAACA GTGGCCGCTT 
ATTTCTATAG AACGCTCCTT GGAGCTACTG GACTACGCCT ATCCAGATCC GGCAGTGAGA 
CGCTTTGCCA TCCGGTGCTT ACACTTTCTG AAAGACGAAG ACTTGCTCCT GTATCTGCTG 
CAATTGGTAC AGGCCATCAA GCACGAATCG TACCTGGAAA GCGATCTGGT AGTGTTTCTG 
CTGGAGCGGG CACTGCGCAA CCAACGCATC GGCCACTACT TCTTCTGGCA CCTACGCTCT 
GAGATGCAGA CGCCGTCTAT GCAAACACGC TTTGGCCTAC TGCTGGAGGT ATACCTCAAG 
GGTTGCAAAC ATCACGTGGC GCCGCTGCGG AAGCAGCTTC ATGTGCTAGA GAAGCTGAAA 
CAGGGATCTC TGATTGCCAA GAAGGGCAGC AAGGAAAAGG TGAAGACCAT GCTGCAGGAC 
TTTCTGCGGG ACCAGCGCAA CTCGGCTGTC TTTCAGAACA TCCAAAATCC ACTTAATCCC 
AGCTTCCGGT GCAGTGGTGT AACTCCCGAT AGGTGCAAGG TAATGGATAG CAAGATGCGA 
CCGCTATGGG TGGTATTCGA GAATGCTGAC GTCAACGCCA GCGATGTGCA CATCATTTTC 
AAAAACGGTG ACGATCTCCG CCAGGACATG CTCACGTTGC AGATGTTGCG TGTGATGGAC 
CAGCTGTGGA AACGCGATGG TATGGACTTC CGCATGAACA TCTACAACTG CATCAGCATG 
GAGAAGAGCC TGGGCATGAT CGAGGTGGTG CGCCACGCAG AAACCATTGC CAACATACAA 
AAGGAAAAGG GCATGTTCTC CGCCACGTCG CCGTTCAAGA AGGGCTCGCT TCTCAGTTGG 
CTCAAAGAGC ACAACAAGCC CGCCGACAAA CTGAACAAAG CCATCAACGA GTTCACGCTC 
AGCTGTGCAG GCTACTGCGT GGCCACCTAT GTGCTTGGCG TAGCTGATCG ACACTCGGAC 
AATATAATGG TCAAGCGAAA TGGACAGCTT TTTCACATCG ATTTTGGCCA CATCCTGGGC 
CACTTCAAGG AAAAGCTTGG AGTACGTCGA GAACGAGTGC CCTTTGTCTT GACCCACGAC 
TTTGTCTACG TAATCAACAA GGGCTTCAAT GACCGCGAGT CTAAAGAGTT TTGTCATTTT 
CAGGAATTGT GCGAGCGCGC CTTTCTAGTT TTACGCAAAC ACGGCTGCCT TATTCTATCG 
CTGTTCTCAA TGATGATTTC AACGGGACTG CCAGAACTGT CCTCCGAAAA GGATTTGGAC 
TACCTACGGG AAACTTTAGT GCTGGACTAC ACGGAGGAAA AAGCACGCGA ACATTTCCGA 
GCGAAATTCA GCGAAGCTTT GGCCAACTCC TGGAAGACTT CCCTTAACTG GGCCTCGCAC 
AACTTCTCCA AAAACAACAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732536.1
CDS	306..3572
Translation	MNMMDNRALAYVAHQPKYETPPEEAEPPCMRFSVNLWKNEMLNWVDLICLLPNGFLLELRVNPANTIQVIKVEMVNQAKQMPLGYVIKEACEYQVYGISTFNIEPYTDETKRLSEVQPYFGILSLGERTDTTSFSSDYELTKMVNGMIGTTFDHNRTHGSPEIDDFRLYMTQTCDNIELERSAYTWQQRLLYEHPLRLANSTKMPELIRERHPTRTFLIVVKNENDQSTFTLSVNEQDTPFSLTESTLQKMNRSQMKMNDRTSDYILKVSGRDEYLLGDYPLIQFLYIQEMLSDSAVPNVVLQSVYRLESYINHHNEQAMVTKRPLPKKRTVHLHKSISSLWDMGNYFQLTLHSISNVNFDKTRALKVGVHVCLYHGDKKLCAQRSTDSPNGNFDTFLFNDLVMDFDIQMRNLPRMTRLCIVIFEVTKMSRSKKSSNNKDIALKDVPYNKNPLAWVNTTIFDHKDILRTGRHTLYTWTYADDIQSVEVFHPLGTIEPNPRKEECALVDLTFLSSGTGTVRYPSEEVVLQYAADREQVNRLQRQLAGPEKPIKELKELMANYTGLDKIYEMVDQDRNAIWERRNDILRELPEELSILLHCVYWKERDDVADMWYLLKQWPLISIERSLELLDYAYPDPAVRRFAIRCLHFLKDEDLLLYLLQLVQAIKHESYLESDLVVFLLERALRNQRIGHYFFWHLRSEMQTPSMQTRFGLLLEVYLKGCKHHVAPLRKQLHVLEKLKQGSLIAKKGSKEKVKTMLQDFLRDQRNSAVFQNIQNPLNPSFRCSGVTPDRCKVMDSKMRPLWVVFENADVNASDVHIIFKNGDDLRQDMLTLQMLRVMDQLWKRDGMDFRMNIYNCISMEKSLGMIEVVRHAETIANIQKEKGMFSATSPFKKGSLLSWLKEHNKPADKLNKAINEFTLSCAGYCVATYVLGVADRHSDNIMVKRNGQLFHIDFGHILGHFKEKLGVRRERVPFVLTHDFVYVINKGFNDRESKEFCHFQELCERAFLVLRKHGCLILSLFSMMISTGLPELSSEKDLDYLRETLVLDYTEEKAREHFRAKFSEALANSWKTSLNWASHNFSKNNKQ

Target ORF information:

RefSeq Version	NM_169910.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pi3K92E, transcript variant B (Pi3K92E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169910.2

ORF Insert Sequence:

ATGAACATGA TGGACAACCG GGCGTTGGCC TACGTGGCCC ACCAGCCCAA GTATGAGACA 
CCGCCGGAAG AAGCGGAGCC GCCCTGCATG CGCTTTTCGG TTAACCTGTG GAAAAACGAG 
ATGCTGAACT GGGTGGACCT AATCTGCCTG TTGCCCAATG GATTCCTGCT GGAGCTCAGG 
GTCAATCCGG CCAACACCAT CCAGGTAATC AAGGTGGAGA TGGTCAACCA GGCCAAACAG 
ATGCCACTGG GCTATGTGAT CAAAGAGGCC TGCGAGTACC AGGTGTACGG CATCTCGACC 
TTCAACATCG AACCTTACAC CGACGAAACG AAGCGACTCA GTGAGGTCCA GCCGTACTTC 
GGCATCCTCA GCCTCGGCGA GCGCACCGAC ACAACGAGCT TTAGCAGCGA CTACGAGCTG 
ACCAAGATGG TTAACGGAAT GATCGGCACC ACCTTCGATC ATAATCGAAC GCACGGCTCG 
CCCGAGATTG ACGACTTCCG GCTGTATATG ACCCAAACTT GCGACAACAT CGAACTGGAG 
CGCTCCGCCT ACACCTGGCA GCAGAGACTG CTCTACGAGC ATCCTCTGCG ACTGGCGAAC 
TCGACTAAAA TGCCCGAGCT GATACGGGAG CGGCATCCGA CCAGAACCTT TCTCATCGTC 
GTAAAGAACG AGAACGACCA GAGCACGTTT ACGTTGTCGG TGAACGAGCA GGACACTCCC 
TTCTCGCTCA CGGAAAGCAC GCTGCAGAAG ATGAATCGGT CGCAGATGAA AATGAACGAC 
CGGACCTCCG ACTACATACT CAAAGTGAGC GGTCGCGACG AGTACCTGCT TGGCGACTAT 
CCCCTGATTC AGTTCCTGTA CATCCAAGAG ATGCTCTCGG ACTCGGCGGT TCCAAATGTG 
GTGTTGCAAT CAGTTTACCG CCTAGAGTCC TACATCAACC ACCACAACGA GCAGGCCATG 
GTAACCAAAC GACCGCTGCC GAAGAAACGG ACAGTCCACC TCCACAAGTC GATCTCCTCG 
CTGTGGGACA TGGGCAACTA CTTTCAGTTG ACGCTGCACA GCATTTCCAA TGTGAACTTT 
GACAAGACGC GTGCCCTCAA GGTGGGCGTG CATGTGTGCC TATATCACGG CGACAAGAAG 
TTATGCGCCC AGCGATCAAC GGACTCCCCT AACGGCAACT TTGACACGTT CCTGTTTAAT 
GACCTGGTGA TGGACTTTGA CATACAGATG CGCAACCTAC CGCGGATGAC ACGCCTCTGC 
ATTGTGATCT TCGAGGTGAC CAAGATGTCG CGCTCGAAGA AATCGTCCAA CAATAAGGAC 
ATCGCTCTGA AAGACGTCCC GTACAACAAA AACCCGCTGG CCTGGGTCAA CACAACAATT 
TTCGACCACA AGGACATCCT GCGCACCGGT CGCCATACGC TGTACACGTG GACGTATGCT 
GACGACATTC AGTCGGTTGA GGTTTTCCAT CCGCTGGGCA CCATCGAACC CAATCCGCGC 
AAAGAGGAAT GTGCGCTGGT GGACCTCACA TTCCTCAGCA GCGGAACCGG AACGGTGCGG 
TATCCCAGCG AGGAGGTCGT GTTGCAGTAC GCTGCGGATC GAGAGCAGGT CAATCGACTG 
CAACGGCAAC TGGCAGGGCC AGAAAAGCCG ATCAAAGAAC TGAAGGAGCT TATGGCCAAT 
TATACAGGAC TGGATAAGAT CTATGAGATG GTTGACCAGG ACCGCAATGC CATTTGGGAA 
CGCAGAAACG ACATTTTGCG TGAGCTTCCG GAAGAGCTGT CCATCTTACT TCACTGCGTC 
TATTGGAAGG AGCGTGATGA CGTAGCGGAT ATGTGGTATC TTCTCAAACA GTGGCCGCTT 
ATTTCTATAG AACGCTCCTT GGAGCTACTG GACTACGCCT ATCCAGATCC GGCAGTGAGA 
CGCTTTGCCA TCCGGTGCTT ACACTTTCTG AAAGACGAAG ACTTGCTCCT GTATCTGCTG 
CAATTGGTAC AGGCCATCAA GCACGAATCG TACCTGGAAA GCGATCTGGT AGTGTTTCTG 
CTGGAGCGGG CACTGCGCAA CCAACGCATC GGCCACTACT TCTTCTGGCA CCTACGCTCT 
GAGATGCAGA CGCCGTCTAT GCAAACACGC TTTGGCCTAC TGCTGGAGGT ATACCTCAAG 
GGTTGCAAAC ATCACGTGGC GCCGCTGCGG AAGCAGCTTC ATGTGCTAGA GAAGCTGAAA 
CAGGGATCTC TGATTGCCAA GAAGGGCAGC AAGGAAAAGG TGAAGACCAT GCTGCAGGAC 
TTTCTGCGGG ACCAGCGCAA CTCGGCTGTC TTTCAGAACA TCCAAAATCC ACTTAATCCC 
AGCTTCCGGT GCAGTGGTGT AACTCCCGAT AGGTGCAAGG TAATGGATAG CAAGATGCGA 
CCGCTATGGG TGGTATTCGA GAATGCTGAC GTCAACGCCA GCGATGTGCA CATCATTTTC 
AAAAACGGTG ACGATCTCCG CCAGGACATG CTCACGTTGC AGATGTTGCG TGTGATGGAC 
CAGCTGTGGA AACGCGATGG TATGGACTTC CGCATGAACA TCTACAACTG CATCAGCATG 
GAGAAGAGCC TGGGCATGAT CGAGGTGGTG CGCCACGCAG AAACCATTGC CAACATACAA 
AAGGAAAAGG GCATGTTCTC CGCCACGTCG CCGTTCAAGA AGGGCTCGCT TCTCAGTTGG 
CTCAAAGAGC ACAACAAGCC CGCCGACAAA CTGAACAAAG CCATCAACGA GTTCACGCTC 
AGCTGTGCAG GCTACTGCGT GGCCACCTAT GTGCTTGGCG TAGCTGATCG ACACTCGGAC 
AATATAATGG TCAAGCGAAA TGGACAGCTT TTTCACATCG ATTTTGGCCA CATCCTGGGC 
CACTTCAAGG AAAAGCTTGG AGTACGTCGA GAACGAGTGC CCTTTGTCTT GACCCACGAC 
TTTGTCTACG TAATCAACAA GGGCTTCAAT GACCGCGAGT CTAAAGAGTT TTGTCATTTT 
CAGGAATTGT GCGAGCGCGC CTTTCTAGTT TTACGCAAAC ACGGCTGCCT TATTCTATCG 
CTGTTCTCAA TGATGATTTC AACGGGACTG CCAGAACTGT CCTCCGAAAA GGATTTGGAC 
TACCTACGGG AAACTTTAGT GCTGGACTAC ACGGAGGAAA AAGCACGCGA ACATTTCCGA 
GCGAAATTCA GCGAAGCTTT GGCCAACTCC TGGAAGACTT CCCTTAACTG GGCCTCGCAC 
AACTTCTCCA AAAACAACAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi3K92E ( NM_001275841.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262770.1 Drosophila melanogaster Pi3K92E, transcript variant C (Pi3K92E), mRNA.

Price & Availability↑

CloneID	OFa05496
Clone ID Related Accession (Same CDS sequence)	NM_169910.2 , NM_001275841.1 , NM_142645.3
Accession Version	NM_001275841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Pi3K92E, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACATGA TGGACAACCG GGCGTTGGCC TACGTGGCCC ACCAGCCCAA GTATGAGACA 
CCGCCGGAAG AAGCGGAGCC GCCCTGCATG CGCTTTTCGG TTAACCTGTG GAAAAACGAG 
ATGCTGAACT GGGTGGACCT AATCTGCCTG TTGCCCAATG GATTCCTGCT GGAGCTCAGG 
GTCAATCCGG CCAACACCAT CCAGGTAATC AAGGTGGAGA TGGTCAACCA GGCCAAACAG 
ATGCCACTGG GCTATGTGAT CAAAGAGGCC TGCGAGTACC AGGTGTACGG CATCTCGACC 
TTCAACATCG AACCTTACAC CGACGAAACG AAGCGACTCA GTGAGGTCCA GCCGTACTTC 
GGCATCCTCA GCCTCGGCGA GCGCACCGAC ACAACGAGCT TTAGCAGCGA CTACGAGCTG 
ACCAAGATGG TTAACGGAAT GATCGGCACC ACCTTCGATC ATAATCGAAC GCACGGCTCG 
CCCGAGATTG ACGACTTCCG GCTGTATATG ACCCAAACTT GCGACAACAT CGAACTGGAG 
CGCTCCGCCT ACACCTGGCA GCAGAGACTG CTCTACGAGC ATCCTCTGCG ACTGGCGAAC 
TCGACTAAAA TGCCCGAGCT GATACGGGAG CGGCATCCGA CCAGAACCTT TCTCATCGTC 
GTAAAGAACG AGAACGACCA GAGCACGTTT ACGTTGTCGG TGAACGAGCA GGACACTCCC 
TTCTCGCTCA CGGAAAGCAC GCTGCAGAAG ATGAATCGGT CGCAGATGAA AATGAACGAC 
CGGACCTCCG ACTACATACT CAAAGTGAGC GGTCGCGACG AGTACCTGCT TGGCGACTAT 
CCCCTGATTC AGTTCCTGTA CATCCAAGAG ATGCTCTCGG ACTCGGCGGT TCCAAATGTG 
GTGTTGCAAT CAGTTTACCG CCTAGAGTCC TACATCAACC ACCACAACGA GCAGGCCATG 
GTAACCAAAC GACCGCTGCC GAAGAAACGG ACAGTCCACC TCCACAAGTC GATCTCCTCG 
CTGTGGGACA TGGGCAACTA CTTTCAGTTG ACGCTGCACA GCATTTCCAA TGTGAACTTT 
GACAAGACGC GTGCCCTCAA GGTGGGCGTG CATGTGTGCC TATATCACGG CGACAAGAAG 
TTATGCGCCC AGCGATCAAC GGACTCCCCT AACGGCAACT TTGACACGTT CCTGTTTAAT 
GACCTGGTGA TGGACTTTGA CATACAGATG CGCAACCTAC CGCGGATGAC ACGCCTCTGC 
ATTGTGATCT TCGAGGTGAC CAAGATGTCG CGCTCGAAGA AATCGTCCAA CAATAAGGAC 
ATCGCTCTGA AAGACGTCCC GTACAACAAA AACCCGCTGG CCTGGGTCAA CACAACAATT 
TTCGACCACA AGGACATCCT GCGCACCGGT CGCCATACGC TGTACACGTG GACGTATGCT 
GACGACATTC AGTCGGTTGA GGTTTTCCAT CCGCTGGGCA CCATCGAACC CAATCCGCGC 
AAAGAGGAAT GTGCGCTGGT GGACCTCACA TTCCTCAGCA GCGGAACCGG AACGGTGCGG 
TATCCCAGCG AGGAGGTCGT GTTGCAGTAC GCTGCGGATC GAGAGCAGGT CAATCGACTG 
CAACGGCAAC TGGCAGGGCC AGAAAAGCCG ATCAAAGAAC TGAAGGAGCT TATGGCCAAT 
TATACAGGAC TGGATAAGAT CTATGAGATG GTTGACCAGG ACCGCAATGC CATTTGGGAA 
CGCAGAAACG ACATTTTGCG TGAGCTTCCG GAAGAGCTGT CCATCTTACT TCACTGCGTC 
TATTGGAAGG AGCGTGATGA CGTAGCGGAT ATGTGGTATC TTCTCAAACA GTGGCCGCTT 
ATTTCTATAG AACGCTCCTT GGAGCTACTG GACTACGCCT ATCCAGATCC GGCAGTGAGA 
CGCTTTGCCA TCCGGTGCTT ACACTTTCTG AAAGACGAAG ACTTGCTCCT GTATCTGCTG 
CAATTGGTAC AGGCCATCAA GCACGAATCG TACCTGGAAA GCGATCTGGT AGTGTTTCTG 
CTGGAGCGGG CACTGCGCAA CCAACGCATC GGCCACTACT TCTTCTGGCA CCTACGCTCT 
GAGATGCAGA CGCCGTCTAT GCAAACACGC TTTGGCCTAC TGCTGGAGGT ATACCTCAAG 
GGTTGCAAAC ATCACGTGGC GCCGCTGCGG AAGCAGCTTC ATGTGCTAGA GAAGCTGAAA 
CAGGGATCTC TGATTGCCAA GAAGGGCAGC AAGGAAAAGG TGAAGACCAT GCTGCAGGAC 
TTTCTGCGGG ACCAGCGCAA CTCGGCTGTC TTTCAGAACA TCCAAAATCC ACTTAATCCC 
AGCTTCCGGT GCAGTGGTGT AACTCCCGAT AGGTGCAAGG TAATGGATAG CAAGATGCGA 
CCGCTATGGG TGGTATTCGA GAATGCTGAC GTCAACGCCA GCGATGTGCA CATCATTTTC 
AAAAACGGTG ACGATCTCCG CCAGGACATG CTCACGTTGC AGATGTTGCG TGTGATGGAC 
CAGCTGTGGA AACGCGATGG TATGGACTTC CGCATGAACA TCTACAACTG CATCAGCATG 
GAGAAGAGCC TGGGCATGAT CGAGGTGGTG CGCCACGCAG AAACCATTGC CAACATACAA 
AAGGAAAAGG GCATGTTCTC CGCCACGTCG CCGTTCAAGA AGGGCTCGCT TCTCAGTTGG 
CTCAAAGAGC ACAACAAGCC CGCCGACAAA CTGAACAAAG CCATCAACGA GTTCACGCTC 
AGCTGTGCAG GCTACTGCGT GGCCACCTAT GTGCTTGGCG TAGCTGATCG ACACTCGGAC 
AATATAATGG TCAAGCGAAA TGGACAGCTT TTTCACATCG ATTTTGGCCA CATCCTGGGC 
CACTTCAAGG AAAAGCTTGG AGTACGTCGA GAACGAGTGC CCTTTGTCTT GACCCACGAC 
TTTGTCTACG TAATCAACAA GGGCTTCAAT GACCGCGAGT CTAAAGAGTT TTGTCATTTT 
CAGGAATTGT GCGAGCGCGC CTTTCTAGTT TTACGCAAAC ACGGCTGCCT TATTCTATCG 
CTGTTCTCAA TGATGATTTC AACGGGACTG CCAGAACTGT CCTCCGAAAA GGATTTGGAC 
TACCTACGGG AAACTTTAGT GCTGGACTAC ACGGAGGAAA AAGCACGCGA ACATTTCCGA 
GCGAAATTCA GCGAAGCTTT GGCCAACTCC TGGAAGACTT CCCTTAACTG GGCCTCGCAC 
AACTTCTCCA AAAACAACAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262770.1
CDS	798..4064
Translation	MNMMDNRALAYVAHQPKYETPPEEAEPPCMRFSVNLWKNEMLNWVDLICLLPNGFLLELRVNPANTIQVIKVEMVNQAKQMPLGYVIKEACEYQVYGISTFNIEPYTDETKRLSEVQPYFGILSLGERTDTTSFSSDYELTKMVNGMIGTTFDHNRTHGSPEIDDFRLYMTQTCDNIELERSAYTWQQRLLYEHPLRLANSTKMPELIRERHPTRTFLIVVKNENDQSTFTLSVNEQDTPFSLTESTLQKMNRSQMKMNDRTSDYILKVSGRDEYLLGDYPLIQFLYIQEMLSDSAVPNVVLQSVYRLESYINHHNEQAMVTKRPLPKKRTVHLHKSISSLWDMGNYFQLTLHSISNVNFDKTRALKVGVHVCLYHGDKKLCAQRSTDSPNGNFDTFLFNDLVMDFDIQMRNLPRMTRLCIVIFEVTKMSRSKKSSNNKDIALKDVPYNKNPLAWVNTTIFDHKDILRTGRHTLYTWTYADDIQSVEVFHPLGTIEPNPRKEECALVDLTFLSSGTGTVRYPSEEVVLQYAADREQVNRLQRQLAGPEKPIKELKELMANYTGLDKIYEMVDQDRNAIWERRNDILRELPEELSILLHCVYWKERDDVADMWYLLKQWPLISIERSLELLDYAYPDPAVRRFAIRCLHFLKDEDLLLYLLQLVQAIKHESYLESDLVVFLLERALRNQRIGHYFFWHLRSEMQTPSMQTRFGLLLEVYLKGCKHHVAPLRKQLHVLEKLKQGSLIAKKGSKEKVKTMLQDFLRDQRNSAVFQNIQNPLNPSFRCSGVTPDRCKVMDSKMRPLWVVFENADVNASDVHIIFKNGDDLRQDMLTLQMLRVMDQLWKRDGMDFRMNIYNCISMEKSLGMIEVVRHAETIANIQKEKGMFSATSPFKKGSLLSWLKEHNKPADKLNKAINEFTLSCAGYCVATYVLGVADRHSDNIMVKRNGQLFHIDFGHILGHFKEKLGVRRERVPFVLTHDFVYVINKGFNDRESKEFCHFQELCERAFLVLRKHGCLILSLFSMMISTGLPELSSEKDLDYLRETLVLDYTEEKAREHFRAKFSEALANSWKTSLNWASHNFSKNNKQ

Target ORF information:

RefSeq Version	NM_001275841.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pi3K92E, transcript variant C (Pi3K92E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275841.1

ORF Insert Sequence:

ATGAACATGA TGGACAACCG GGCGTTGGCC TACGTGGCCC ACCAGCCCAA GTATGAGACA 
CCGCCGGAAG AAGCGGAGCC GCCCTGCATG CGCTTTTCGG TTAACCTGTG GAAAAACGAG 
ATGCTGAACT GGGTGGACCT AATCTGCCTG TTGCCCAATG GATTCCTGCT GGAGCTCAGG 
GTCAATCCGG CCAACACCAT CCAGGTAATC AAGGTGGAGA TGGTCAACCA GGCCAAACAG 
ATGCCACTGG GCTATGTGAT CAAAGAGGCC TGCGAGTACC AGGTGTACGG CATCTCGACC 
TTCAACATCG AACCTTACAC CGACGAAACG AAGCGACTCA GTGAGGTCCA GCCGTACTTC 
GGCATCCTCA GCCTCGGCGA GCGCACCGAC ACAACGAGCT TTAGCAGCGA CTACGAGCTG 
ACCAAGATGG TTAACGGAAT GATCGGCACC ACCTTCGATC ATAATCGAAC GCACGGCTCG 
CCCGAGATTG ACGACTTCCG GCTGTATATG ACCCAAACTT GCGACAACAT CGAACTGGAG 
CGCTCCGCCT ACACCTGGCA GCAGAGACTG CTCTACGAGC ATCCTCTGCG ACTGGCGAAC 
TCGACTAAAA TGCCCGAGCT GATACGGGAG CGGCATCCGA CCAGAACCTT TCTCATCGTC 
GTAAAGAACG AGAACGACCA GAGCACGTTT ACGTTGTCGG TGAACGAGCA GGACACTCCC 
TTCTCGCTCA CGGAAAGCAC GCTGCAGAAG ATGAATCGGT CGCAGATGAA AATGAACGAC 
CGGACCTCCG ACTACATACT CAAAGTGAGC GGTCGCGACG AGTACCTGCT TGGCGACTAT 
CCCCTGATTC AGTTCCTGTA CATCCAAGAG ATGCTCTCGG ACTCGGCGGT TCCAAATGTG 
GTGTTGCAAT CAGTTTACCG CCTAGAGTCC TACATCAACC ACCACAACGA GCAGGCCATG 
GTAACCAAAC GACCGCTGCC GAAGAAACGG ACAGTCCACC TCCACAAGTC GATCTCCTCG 
CTGTGGGACA TGGGCAACTA CTTTCAGTTG ACGCTGCACA GCATTTCCAA TGTGAACTTT 
GACAAGACGC GTGCCCTCAA GGTGGGCGTG CATGTGTGCC TATATCACGG CGACAAGAAG 
TTATGCGCCC AGCGATCAAC GGACTCCCCT AACGGCAACT TTGACACGTT CCTGTTTAAT 
GACCTGGTGA TGGACTTTGA CATACAGATG CGCAACCTAC CGCGGATGAC ACGCCTCTGC 
ATTGTGATCT TCGAGGTGAC CAAGATGTCG CGCTCGAAGA AATCGTCCAA CAATAAGGAC 
ATCGCTCTGA AAGACGTCCC GTACAACAAA AACCCGCTGG CCTGGGTCAA CACAACAATT 
TTCGACCACA AGGACATCCT GCGCACCGGT CGCCATACGC TGTACACGTG GACGTATGCT 
GACGACATTC AGTCGGTTGA GGTTTTCCAT CCGCTGGGCA CCATCGAACC CAATCCGCGC 
AAAGAGGAAT GTGCGCTGGT GGACCTCACA TTCCTCAGCA GCGGAACCGG AACGGTGCGG 
TATCCCAGCG AGGAGGTCGT GTTGCAGTAC GCTGCGGATC GAGAGCAGGT CAATCGACTG 
CAACGGCAAC TGGCAGGGCC AGAAAAGCCG ATCAAAGAAC TGAAGGAGCT TATGGCCAAT 
TATACAGGAC TGGATAAGAT CTATGAGATG GTTGACCAGG ACCGCAATGC CATTTGGGAA 
CGCAGAAACG ACATTTTGCG TGAGCTTCCG GAAGAGCTGT CCATCTTACT TCACTGCGTC 
TATTGGAAGG AGCGTGATGA CGTAGCGGAT ATGTGGTATC TTCTCAAACA GTGGCCGCTT 
ATTTCTATAG AACGCTCCTT GGAGCTACTG GACTACGCCT ATCCAGATCC GGCAGTGAGA 
CGCTTTGCCA TCCGGTGCTT ACACTTTCTG AAAGACGAAG ACTTGCTCCT GTATCTGCTG 
CAATTGGTAC AGGCCATCAA GCACGAATCG TACCTGGAAA GCGATCTGGT AGTGTTTCTG 
CTGGAGCGGG CACTGCGCAA CCAACGCATC GGCCACTACT TCTTCTGGCA CCTACGCTCT 
GAGATGCAGA CGCCGTCTAT GCAAACACGC TTTGGCCTAC TGCTGGAGGT ATACCTCAAG 
GGTTGCAAAC ATCACGTGGC GCCGCTGCGG AAGCAGCTTC ATGTGCTAGA GAAGCTGAAA 
CAGGGATCTC TGATTGCCAA GAAGGGCAGC AAGGAAAAGG TGAAGACCAT GCTGCAGGAC 
TTTCTGCGGG ACCAGCGCAA CTCGGCTGTC TTTCAGAACA TCCAAAATCC ACTTAATCCC 
AGCTTCCGGT GCAGTGGTGT AACTCCCGAT AGGTGCAAGG TAATGGATAG CAAGATGCGA 
CCGCTATGGG TGGTATTCGA GAATGCTGAC GTCAACGCCA GCGATGTGCA CATCATTTTC 
AAAAACGGTG ACGATCTCCG CCAGGACATG CTCACGTTGC AGATGTTGCG TGTGATGGAC 
CAGCTGTGGA AACGCGATGG TATGGACTTC CGCATGAACA TCTACAACTG CATCAGCATG 
GAGAAGAGCC TGGGCATGAT CGAGGTGGTG CGCCACGCAG AAACCATTGC CAACATACAA 
AAGGAAAAGG GCATGTTCTC CGCCACGTCG CCGTTCAAGA AGGGCTCGCT TCTCAGTTGG 
CTCAAAGAGC ACAACAAGCC CGCCGACAAA CTGAACAAAG CCATCAACGA GTTCACGCTC 
AGCTGTGCAG GCTACTGCGT GGCCACCTAT GTGCTTGGCG TAGCTGATCG ACACTCGGAC 
AATATAATGG TCAAGCGAAA TGGACAGCTT TTTCACATCG ATTTTGGCCA CATCCTGGGC 
CACTTCAAGG AAAAGCTTGG AGTACGTCGA GAACGAGTGC CCTTTGTCTT GACCCACGAC 
TTTGTCTACG TAATCAACAA GGGCTTCAAT GACCGCGAGT CTAAAGAGTT TTGTCATTTT 
CAGGAATTGT GCGAGCGCGC CTTTCTAGTT TTACGCAAAC ACGGCTGCCT TATTCTATCG 
CTGTTCTCAA TGATGATTTC AACGGGACTG CCAGAACTGT CCTCCGAAAA GGATTTGGAC 
TACCTACGGG AAACTTTAGT GCTGGACTAC ACGGAGGAAA AAGCACGCGA ACATTTCCGA 
GCGAAATTCA GCGAAGCTTT GGCCAACTCC TGGAAGACTT CCCTTAACTG GGCCTCGCAC 
AACTTCTCCA AAAACAACAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi3K92E ( NM_142645.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650902.1 Drosophila melanogaster Pi3K92E, transcript variant A (Pi3K92E), mRNA.

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CloneID	OFa05496
Clone ID Related Accession (Same CDS sequence)	NM_169910.2 , NM_001275841.1 , NM_142645.3
Accession Version	NM_142645.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Pi3K92E, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142645.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACATGA TGGACAACCG GGCGTTGGCC TACGTGGCCC ACCAGCCCAA GTATGAGACA 
CCGCCGGAAG AAGCGGAGCC GCCCTGCATG CGCTTTTCGG TTAACCTGTG GAAAAACGAG 
ATGCTGAACT GGGTGGACCT AATCTGCCTG TTGCCCAATG GATTCCTGCT GGAGCTCAGG 
GTCAATCCGG CCAACACCAT CCAGGTAATC AAGGTGGAGA TGGTCAACCA GGCCAAACAG 
ATGCCACTGG GCTATGTGAT CAAAGAGGCC TGCGAGTACC AGGTGTACGG CATCTCGACC 
TTCAACATCG AACCTTACAC CGACGAAACG AAGCGACTCA GTGAGGTCCA GCCGTACTTC 
GGCATCCTCA GCCTCGGCGA GCGCACCGAC ACAACGAGCT TTAGCAGCGA CTACGAGCTG 
ACCAAGATGG TTAACGGAAT GATCGGCACC ACCTTCGATC ATAATCGAAC GCACGGCTCG 
CCCGAGATTG ACGACTTCCG GCTGTATATG ACCCAAACTT GCGACAACAT CGAACTGGAG 
CGCTCCGCCT ACACCTGGCA GCAGAGACTG CTCTACGAGC ATCCTCTGCG ACTGGCGAAC 
TCGACTAAAA TGCCCGAGCT GATACGGGAG CGGCATCCGA CCAGAACCTT TCTCATCGTC 
GTAAAGAACG AGAACGACCA GAGCACGTTT ACGTTGTCGG TGAACGAGCA GGACACTCCC 
TTCTCGCTCA CGGAAAGCAC GCTGCAGAAG ATGAATCGGT CGCAGATGAA AATGAACGAC 
CGGACCTCCG ACTACATACT CAAAGTGAGC GGTCGCGACG AGTACCTGCT TGGCGACTAT 
CCCCTGATTC AGTTCCTGTA CATCCAAGAG ATGCTCTCGG ACTCGGCGGT TCCAAATGTG 
GTGTTGCAAT CAGTTTACCG CCTAGAGTCC TACATCAACC ACCACAACGA GCAGGCCATG 
GTAACCAAAC GACCGCTGCC GAAGAAACGG ACAGTCCACC TCCACAAGTC GATCTCCTCG 
CTGTGGGACA TGGGCAACTA CTTTCAGTTG ACGCTGCACA GCATTTCCAA TGTGAACTTT 
GACAAGACGC GTGCCCTCAA GGTGGGCGTG CATGTGTGCC TATATCACGG CGACAAGAAG 
TTATGCGCCC AGCGATCAAC GGACTCCCCT AACGGCAACT TTGACACGTT CCTGTTTAAT 
GACCTGGTGA TGGACTTTGA CATACAGATG CGCAACCTAC CGCGGATGAC ACGCCTCTGC 
ATTGTGATCT TCGAGGTGAC CAAGATGTCG CGCTCGAAGA AATCGTCCAA CAATAAGGAC 
ATCGCTCTGA AAGACGTCCC GTACAACAAA AACCCGCTGG CCTGGGTCAA CACAACAATT 
TTCGACCACA AGGACATCCT GCGCACCGGT CGCCATACGC TGTACACGTG GACGTATGCT 
GACGACATTC AGTCGGTTGA GGTTTTCCAT CCGCTGGGCA CCATCGAACC CAATCCGCGC 
AAAGAGGAAT GTGCGCTGGT GGACCTCACA TTCCTCAGCA GCGGAACCGG AACGGTGCGG 
TATCCCAGCG AGGAGGTCGT GTTGCAGTAC GCTGCGGATC GAGAGCAGGT CAATCGACTG 
CAACGGCAAC TGGCAGGGCC AGAAAAGCCG ATCAAAGAAC TGAAGGAGCT TATGGCCAAT 
TATACAGGAC TGGATAAGAT CTATGAGATG GTTGACCAGG ACCGCAATGC CATTTGGGAA 
CGCAGAAACG ACATTTTGCG TGAGCTTCCG GAAGAGCTGT CCATCTTACT TCACTGCGTC 
TATTGGAAGG AGCGTGATGA CGTAGCGGAT ATGTGGTATC TTCTCAAACA GTGGCCGCTT 
ATTTCTATAG AACGCTCCTT GGAGCTACTG GACTACGCCT ATCCAGATCC GGCAGTGAGA 
CGCTTTGCCA TCCGGTGCTT ACACTTTCTG AAAGACGAAG ACTTGCTCCT GTATCTGCTG 
CAATTGGTAC AGGCCATCAA GCACGAATCG TACCTGGAAA GCGATCTGGT AGTGTTTCTG 
CTGGAGCGGG CACTGCGCAA CCAACGCATC GGCCACTACT TCTTCTGGCA CCTACGCTCT 
GAGATGCAGA CGCCGTCTAT GCAAACACGC TTTGGCCTAC TGCTGGAGGT ATACCTCAAG 
GGTTGCAAAC ATCACGTGGC GCCGCTGCGG AAGCAGCTTC ATGTGCTAGA GAAGCTGAAA 
CAGGGATCTC TGATTGCCAA GAAGGGCAGC AAGGAAAAGG TGAAGACCAT GCTGCAGGAC 
TTTCTGCGGG ACCAGCGCAA CTCGGCTGTC TTTCAGAACA TCCAAAATCC ACTTAATCCC 
AGCTTCCGGT GCAGTGGTGT AACTCCCGAT AGGTGCAAGG TAATGGATAG CAAGATGCGA 
CCGCTATGGG TGGTATTCGA GAATGCTGAC GTCAACGCCA GCGATGTGCA CATCATTTTC 
AAAAACGGTG ACGATCTCCG CCAGGACATG CTCACGTTGC AGATGTTGCG TGTGATGGAC 
CAGCTGTGGA AACGCGATGG TATGGACTTC CGCATGAACA TCTACAACTG CATCAGCATG 
GAGAAGAGCC TGGGCATGAT CGAGGTGGTG CGCCACGCAG AAACCATTGC CAACATACAA 
AAGGAAAAGG GCATGTTCTC CGCCACGTCG CCGTTCAAGA AGGGCTCGCT TCTCAGTTGG 
CTCAAAGAGC ACAACAAGCC CGCCGACAAA CTGAACAAAG CCATCAACGA GTTCACGCTC 
AGCTGTGCAG GCTACTGCGT GGCCACCTAT GTGCTTGGCG TAGCTGATCG ACACTCGGAC 
AATATAATGG TCAAGCGAAA TGGACAGCTT TTTCACATCG ATTTTGGCCA CATCCTGGGC 
CACTTCAAGG AAAAGCTTGG AGTACGTCGA GAACGAGTGC CCTTTGTCTT GACCCACGAC 
TTTGTCTACG TAATCAACAA GGGCTTCAAT GACCGCGAGT CTAAAGAGTT TTGTCATTTT 
CAGGAATTGT GCGAGCGCGC CTTTCTAGTT TTACGCAAAC ACGGCTGCCT TATTCTATCG 
CTGTTCTCAA TGATGATTTC AACGGGACTG CCAGAACTGT CCTCCGAAAA GGATTTGGAC 
TACCTACGGG AAACTTTAGT GCTGGACTAC ACGGAGGAAA AAGCACGCGA ACATTTCCGA 
GCGAAATTCA GCGAAGCTTT GGCCAACTCC TGGAAGACTT CCCTTAACTG GGCCTCGCAC 
AACTTCTCCA AAAACAACAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650902.1
CDS	379..3645
Translation	MNMMDNRALAYVAHQPKYETPPEEAEPPCMRFSVNLWKNEMLNWVDLICLLPNGFLLELRVNPANTIQVIKVEMVNQAKQMPLGYVIKEACEYQVYGISTFNIEPYTDETKRLSEVQPYFGILSLGERTDTTSFSSDYELTKMVNGMIGTTFDHNRTHGSPEIDDFRLYMTQTCDNIELERSAYTWQQRLLYEHPLRLANSTKMPELIRERHPTRTFLIVVKNENDQSTFTLSVNEQDTPFSLTESTLQKMNRSQMKMNDRTSDYILKVSGRDEYLLGDYPLIQFLYIQEMLSDSAVPNVVLQSVYRLESYINHHNEQAMVTKRPLPKKRTVHLHKSISSLWDMGNYFQLTLHSISNVNFDKTRALKVGVHVCLYHGDKKLCAQRSTDSPNGNFDTFLFNDLVMDFDIQMRNLPRMTRLCIVIFEVTKMSRSKKSSNNKDIALKDVPYNKNPLAWVNTTIFDHKDILRTGRHTLYTWTYADDIQSVEVFHPLGTIEPNPRKEECALVDLTFLSSGTGTVRYPSEEVVLQYAADREQVNRLQRQLAGPEKPIKELKELMANYTGLDKIYEMVDQDRNAIWERRNDILRELPEELSILLHCVYWKERDDVADMWYLLKQWPLISIERSLELLDYAYPDPAVRRFAIRCLHFLKDEDLLLYLLQLVQAIKHESYLESDLVVFLLERALRNQRIGHYFFWHLRSEMQTPSMQTRFGLLLEVYLKGCKHHVAPLRKQLHVLEKLKQGSLIAKKGSKEKVKTMLQDFLRDQRNSAVFQNIQNPLNPSFRCSGVTPDRCKVMDSKMRPLWVVFENADVNASDVHIIFKNGDDLRQDMLTLQMLRVMDQLWKRDGMDFRMNIYNCISMEKSLGMIEVVRHAETIANIQKEKGMFSATSPFKKGSLLSWLKEHNKPADKLNKAINEFTLSCAGYCVATYVLGVADRHSDNIMVKRNGQLFHIDFGHILGHFKEKLGVRRERVPFVLTHDFVYVINKGFNDRESKEFCHFQELCERAFLVLRKHGCLILSLFSMMISTGLPELSSEKDLDYLRETLVLDYTEEKAREHFRAKFSEALANSWKTSLNWASHNFSKNNKQ

Target ORF information:

RefSeq Version	NM_142645.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pi3K92E, transcript variant A (Pi3K92E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142645.3

ORF Insert Sequence:

ATGAACATGA TGGACAACCG GGCGTTGGCC TACGTGGCCC ACCAGCCCAA GTATGAGACA 
CCGCCGGAAG AAGCGGAGCC GCCCTGCATG CGCTTTTCGG TTAACCTGTG GAAAAACGAG 
ATGCTGAACT GGGTGGACCT AATCTGCCTG TTGCCCAATG GATTCCTGCT GGAGCTCAGG 
GTCAATCCGG CCAACACCAT CCAGGTAATC AAGGTGGAGA TGGTCAACCA GGCCAAACAG 
ATGCCACTGG GCTATGTGAT CAAAGAGGCC TGCGAGTACC AGGTGTACGG CATCTCGACC 
TTCAACATCG AACCTTACAC CGACGAAACG AAGCGACTCA GTGAGGTCCA GCCGTACTTC 
GGCATCCTCA GCCTCGGCGA GCGCACCGAC ACAACGAGCT TTAGCAGCGA CTACGAGCTG 
ACCAAGATGG TTAACGGAAT GATCGGCACC ACCTTCGATC ATAATCGAAC GCACGGCTCG 
CCCGAGATTG ACGACTTCCG GCTGTATATG ACCCAAACTT GCGACAACAT CGAACTGGAG 
CGCTCCGCCT ACACCTGGCA GCAGAGACTG CTCTACGAGC ATCCTCTGCG ACTGGCGAAC 
TCGACTAAAA TGCCCGAGCT GATACGGGAG CGGCATCCGA CCAGAACCTT TCTCATCGTC 
GTAAAGAACG AGAACGACCA GAGCACGTTT ACGTTGTCGG TGAACGAGCA GGACACTCCC 
TTCTCGCTCA CGGAAAGCAC GCTGCAGAAG ATGAATCGGT CGCAGATGAA AATGAACGAC 
CGGACCTCCG ACTACATACT CAAAGTGAGC GGTCGCGACG AGTACCTGCT TGGCGACTAT 
CCCCTGATTC AGTTCCTGTA CATCCAAGAG ATGCTCTCGG ACTCGGCGGT TCCAAATGTG 
GTGTTGCAAT CAGTTTACCG CCTAGAGTCC TACATCAACC ACCACAACGA GCAGGCCATG 
GTAACCAAAC GACCGCTGCC GAAGAAACGG ACAGTCCACC TCCACAAGTC GATCTCCTCG 
CTGTGGGACA TGGGCAACTA CTTTCAGTTG ACGCTGCACA GCATTTCCAA TGTGAACTTT 
GACAAGACGC GTGCCCTCAA GGTGGGCGTG CATGTGTGCC TATATCACGG CGACAAGAAG 
TTATGCGCCC AGCGATCAAC GGACTCCCCT AACGGCAACT TTGACACGTT CCTGTTTAAT 
GACCTGGTGA TGGACTTTGA CATACAGATG CGCAACCTAC CGCGGATGAC ACGCCTCTGC 
ATTGTGATCT TCGAGGTGAC CAAGATGTCG CGCTCGAAGA AATCGTCCAA CAATAAGGAC 
ATCGCTCTGA AAGACGTCCC GTACAACAAA AACCCGCTGG CCTGGGTCAA CACAACAATT 
TTCGACCACA AGGACATCCT GCGCACCGGT CGCCATACGC TGTACACGTG GACGTATGCT 
GACGACATTC AGTCGGTTGA GGTTTTCCAT CCGCTGGGCA CCATCGAACC CAATCCGCGC 
AAAGAGGAAT GTGCGCTGGT GGACCTCACA TTCCTCAGCA GCGGAACCGG AACGGTGCGG 
TATCCCAGCG AGGAGGTCGT GTTGCAGTAC GCTGCGGATC GAGAGCAGGT CAATCGACTG 
CAACGGCAAC TGGCAGGGCC AGAAAAGCCG ATCAAAGAAC TGAAGGAGCT TATGGCCAAT 
TATACAGGAC TGGATAAGAT CTATGAGATG GTTGACCAGG ACCGCAATGC CATTTGGGAA 
CGCAGAAACG ACATTTTGCG TGAGCTTCCG GAAGAGCTGT CCATCTTACT TCACTGCGTC 
TATTGGAAGG AGCGTGATGA CGTAGCGGAT ATGTGGTATC TTCTCAAACA GTGGCCGCTT 
ATTTCTATAG AACGCTCCTT GGAGCTACTG GACTACGCCT ATCCAGATCC GGCAGTGAGA 
CGCTTTGCCA TCCGGTGCTT ACACTTTCTG AAAGACGAAG ACTTGCTCCT GTATCTGCTG 
CAATTGGTAC AGGCCATCAA GCACGAATCG TACCTGGAAA GCGATCTGGT AGTGTTTCTG 
CTGGAGCGGG CACTGCGCAA CCAACGCATC GGCCACTACT TCTTCTGGCA CCTACGCTCT 
GAGATGCAGA CGCCGTCTAT GCAAACACGC TTTGGCCTAC TGCTGGAGGT ATACCTCAAG 
GGTTGCAAAC ATCACGTGGC GCCGCTGCGG AAGCAGCTTC ATGTGCTAGA GAAGCTGAAA 
CAGGGATCTC TGATTGCCAA GAAGGGCAGC AAGGAAAAGG TGAAGACCAT GCTGCAGGAC 
TTTCTGCGGG ACCAGCGCAA CTCGGCTGTC TTTCAGAACA TCCAAAATCC ACTTAATCCC 
AGCTTCCGGT GCAGTGGTGT AACTCCCGAT AGGTGCAAGG TAATGGATAG CAAGATGCGA 
CCGCTATGGG TGGTATTCGA GAATGCTGAC GTCAACGCCA GCGATGTGCA CATCATTTTC 
AAAAACGGTG ACGATCTCCG CCAGGACATG CTCACGTTGC AGATGTTGCG TGTGATGGAC 
CAGCTGTGGA AACGCGATGG TATGGACTTC CGCATGAACA TCTACAACTG CATCAGCATG 
GAGAAGAGCC TGGGCATGAT CGAGGTGGTG CGCCACGCAG AAACCATTGC CAACATACAA 
AAGGAAAAGG GCATGTTCTC CGCCACGTCG CCGTTCAAGA AGGGCTCGCT TCTCAGTTGG 
CTCAAAGAGC ACAACAAGCC CGCCGACAAA CTGAACAAAG CCATCAACGA GTTCACGCTC 
AGCTGTGCAG GCTACTGCGT GGCCACCTAT GTGCTTGGCG TAGCTGATCG ACACTCGGAC 
AATATAATGG TCAAGCGAAA TGGACAGCTT TTTCACATCG ATTTTGGCCA CATCCTGGGC 
CACTTCAAGG AAAAGCTTGG AGTACGTCGA GAACGAGTGC CCTTTGTCTT GACCCACGAC 
TTTGTCTACG TAATCAACAA GGGCTTCAAT GACCGCGAGT CTAAAGAGTT TTGTCATTTT 
CAGGAATTGT GCGAGCGCGC CTTTCTAGTT TTACGCAAAC ACGGCTGCCT TATTCTATCG 
CTGTTCTCAA TGATGATTTC AACGGGACTG CCAGAACTGT CCTCCGAAAA GGATTTGGAC 
TACCTACGGG AAACTTTAGT GCTGGACTAC ACGGAGGAAA AAGCACGCGA ACATTTCCGA 
GCGAAATTCA GCGAAGCTTT GGCCAACTCC TGGAAGACTT CCCTTAACTG GGCCTCGCAC 
AACTTCTCCA AAAACAACAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pi3K92E cDNA ORF clone, Drosophila melanogaster(fruit fly)

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