psidin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	psidin
Entrez Gene ID	42389
Full Name	phagocyte signaling impaired
Synonyms	CG4845,Dmel\CG4845,ird16,psid
General protein information	Preferred Names phagocyte signaling impaired Names CG4845 gene product from transcript CG4845-RA CG4845-PA immune response deficient 16 psidin-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92C1-92C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_142601.2	NP_650858.1	phagocyte signaling impaired

Pathways from BioSystems

Homologies

	NAA25	XP_001102995.2
Bos taurus (cattle)	NAA25	NP_001193056.1
Danio rerio (zebrafish)	naa25	XP_005172656.1
Anopheles gambiae (African malaria mosquito)	NAA25_ANOGA	XP_321109.5
Caenorhabditis elegans (roundworm)	cra-1	NP_498494.2
Schizosaccharomyces pombe (fission yeast)	naa25	NP_595632.1
Xenopus tropicalis (tropical clawed frog)	naa25	XP_004910562.1
Pan troglodytes (chimpanzee)	NAA25	XP_509384.2
Canis lupus familiaris (dog)	NAA25	XP_543400.1
Drosophila melanogaster (fruit fly)	psidin	NP_650858.1
Homo sapiens (human)	NAA25	NP_079229.2
Mus musculus (house mouse)	Naa25	NP_766310.2
Rattus norvegicus (Norway rat)	Naa25	NP_001041365.1
Gallus gallus (chicken)	NAA25	NP_001025835.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Psidin plays two independent developmental roles via the acquisition of separate signaling pathways, both of which contribute to the formation of olfactory circuits
Title: Drosophila Psidin regulates olfactory neuron number and axon targeting through two distinct molecular mechanisms.

Psid is an actin regulatory protein that plays a conserved role in protrusion dynamics and cell migration.
Title: Psidin, a conserved protein that regulates protrusion dynamics and cell migration.

encodes a lysosomal protein required in the blood cells for both degradation of engulfed bacteria and activation of fat-body Defensin
Title: Psidin is required in Drosophila blood cells for both phagocytic degradation and immune activation of the fat body.

The following psidin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the psidin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08562	NM_142601.2 Latest version!	Drosophila melanogaster phagocyte signaling impaired (psidin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

psidin ( NM_142601.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650858.1 Drosophila melanogaster phagocyte signaling impaired (psidin), mRNA.

Price & Availability↑

CloneID	OFa08562
Clone ID Related Accession (Same CDS sequence)	NM_142601.2
Accession Version	NM_142601.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phagocyte signaling impaired
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142601.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACAGC AGCAGGGCAT GGACTCGGCC CTGTTCGAGC GGCGACTGCG GCCGGTATAC 
GACAACCTGG AGGTGGGCAA CAATCGCAAG GCTCTGCAGG AATCCGAGAA GTTGCTACGC 
AAACATCCCA ACCTGCTCTG CGCGCGGGCA TTGAAAGGCC TCTCGCTCCT GCGACTGGGA 
CGCTATGACG AAAGCCACGG ATGCCTCCAG ACCGTTGCCG AGGAGAAGCC CACGGACGAC 
TCCACGCTGC AGGTGCTGTC CTTCTGCTAC CGCGAAATGG AACAACTGGA CAAAATTGTG 
GAGCTGTACC AGCACGCTGT GAAGCAGAAT CCGGGCAACG AGGAGCTCCT CGCCCACCTC 
TTCATCTCAC ATGTGAGGGT GGAGGACTAC AAGGCGCAGC AGGCGGTGGC CCTGCAGCTG 
TACAAGGCGC AGCCGAAAAA TGCCTACTAC TTTTGGTCGG TGATCAGCGT AGTCTTCCAG 
GGAATCCGTG GACCGGAATC GGCGCTGCCC GAGAAGCGGA AAATCTACTT GGGACTGGCG 
CAACGCATGG TCGACAAGCA CATCAAGGAG GGCAAAATGG AGACGGAACA GGAGGCCTTC 
CTCTATCTGC ACATACTTAA GCTACAAAAC AAGTACCAGG AGGCGTGGGA ATTCCTTACT 
GGTGAGCTGT GCGCGAAGCT GTACCCCGGT GCTCCAGTCA GCATGAAGTT CGAGCTGCTC 
AAGGAGCTGG GAAACTGGCG GGAATTGAAT GAGCTGCTGC AGCAGCTGCT GGACGCAGAT 
CGTGATCGTT GGGACTTCTA CAAGGAGTAT ATCCAAAGCT CGTTCGAGCT GCTAAAACTT 
ACGCCGCAGG GCGTGGAGAA CGGGGATAAG GACTCTCTGG CCAGGTGTCA GGAGTTCCTT 
CAGCGCATCA TTGACTCCTC GGAGCGCAAG AAAAGGGGAC CCTATCTGGC GCGGTTGGAG 
CTACACCAAC GCATGCGAGC GGAGCAATTG CCGGCGGAAA AACTAATCGG TGACTTTGAC 
GAGATGGTCA TTGAGTACTT CCGTCTGTTC GGCGACAAAT CGTGCTGCAC TCACGATATC 
GCCTTGTTCT TGCCTTCGAT CAGCATGAAC CAGAGGCAAG CGCTAGCCAG CAAGTTGTTG 
TTGGAGAGCG GAGTAACCTC CACGTCGCTT CCAAAAAATA AAGAGCAGTT GCAAAAACAT 
CTTTGCGCTC TGCAAATCTC TCGAATGTGC GGATCCCACA TGGATCTGCC TGCGGACCAC 
CTGCTTGCCT TCTATACGGC GCTCAAGCTG CACTACGAGC ACGGCAGGAC TACTTTCGGT 
AAGAAGTTGC TGGCTACGGA AATGGGACCA TCGGATGCAT ACGCACTGCT GGCGGCAAAT 
GTAATGTACG ATCTGAGTAG GCGGGAAAAT AAGTCGGATC ACCTGTTCGA GGCGCTGTGC 
CTGCTGCAGT ATGTTCTGCG AAACAGTACG AGTAACTTCC ACGTGAAACT GCTAAGTCTA 
AAGATCTACC ATCTGTTTGG CTGCCAAGTG GGCGCACAGG AGATGTATGA GTATCTGGAC 
ATTAAGCAGA TTCAGCTGGA CTCCATGGGC TATGTGCACT GCCAACTGTT GGCTCTTGGA 
GGTCGCTTCT CGGGCAATCG TAACGTGTAC GATGCCACCC TAAAGTTTTT TACGAACAGC 
TATAAGGAAA GGCTGGAATA CATTGCGTTA ACATATCGCT TTTGCACGTT TTCAAAAATG 
GAGGAGTTCA TGAACTTCAA GGAGCGGTTA ACCAACAGCT TGCAGTACGT GGCCTGCTCG 
GTGGAGGCGC AGATCTGTGA CTTGGTTAGT TGCTATGGCA ATATCACGCA GAATCTATCC 
GCATATGTGG CGATGAGTAT TGAGCCGGCG GAGGACCGGA TCGCTTGGCA TGAGTTGAGC 
GACAATCGCG ACTTAGGGGC CATTATCCGA TGGGATCCGC TACATGAGGT AGACGCTGCG 
GAGGAGCGCA GGGAGTCGTT CGACCAGGAA GTTGAGGTGC TTCAAGTACG TTCGCTGATG 
CTTCGACTCT TTGCCTCTTT CATCGACTTG TACCACGGCG CGGGCTTAAA GGATTCGCCA 
GCGGGAGCAG AGACGGCCAC CTTGGAGCTC CTGCGGGAGT CCTGGACGGG ATTATTCCAG 
AGATTGCGCG TGATGAACTA CAAGCCGTTG GCGCAAAAAT TTCTAGTCAA TCTGCTGCCC 
ACACGTCTTC ACCTGCTGCT CGACATGCCA TACGAACGTT TCTTTGACGA CCTTGCCCAG 
TTGGTCCTTG ACTTGCAGAG CGGGTCGGCA AAGCTGGCCG AGCAGTGCAA GCGGGTGGGT 
GACAATGTTA TTGCGACTAT GGAGCTGTGC GTGCAGACCA TCAACGAGAG CAACGAAAGC 
AACGGCATTG ACGGTCTGTG GAAGCGGCGA GGTCAGCAGC AGAAAGTGGC AGCCAGTTTA 
GAGATGCTAT CGCTGTACGC CTTCCTTCTG TCCGTGTTGA ACGACAAGCT GCAGGTAAGC 
TCAAAGACCA AGACCAAGAA GAAGCCGGTG GAAAACAAGG TGGATGGACC GCAGCCGATC 
AGCGAGAAGG AGCGCAGCCA GATGTTGCAG GAGCTGATGC GACAGCTGAA GCACAAGTTT 
GAGGCCTGCG ACACGGCCAT ACGCTCCTGG AAGGCGCCCG TTTTGCCACG CGACCTCAGC 
TCCTTTATGT CTGACATGTC GCTGAAGCCG GAGGTGGAAG CCACGCTCAT CGGCGACGTT 
TCGGCCACCT TTAAGGACTC GCACGAGCTG ATGGTCACCG AGCTGCGCAA TCTGCTGAAG 
GACAAGATAC GCATGGTGGC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650858.1
CDS	351..3197
Translation	MAQQQGMDSALFERRLRPVYDNLEVGNNRKALQESEKLLRKHPNLLCARALKGLSLLRLGRYDESHGCLQTVAEEKPTDDSTLQVLSFCYREMEQLDKIVELYQHAVKQNPGNEELLAHLFISHVRVEDYKAQQAVALQLYKAQPKNAYYFWSVISVVFQGIRGPESALPEKRKIYLGLAQRMVDKHIKEGKMETEQEAFLYLHILKLQNKYQEAWEFLTGELCAKLYPGAPVSMKFELLKELGNWRELNELLQQLLDADRDRWDFYKEYIQSSFELLKLTPQGVENGDKDSLARCQEFLQRIIDSSERKKRGPYLARLELHQRMRAEQLPAEKLIGDFDEMVIEYFRLFGDKSCCTHDIALFLPSISMNQRQALASKLLLESGVTSTSLPKNKEQLQKHLCALQISRMCGSHMDLPADHLLAFYTALKLHYEHGRTTFGKKLLATEMGPSDAYALLAANVMYDLSRRENKSDHLFEALCLLQYVLRNSTSNFHVKLLSLKIYHLFGCQVGAQEMYEYLDIKQIQLDSMGYVHCQLLALGGRFSGNRNVYDATLKFFTNSYKERLEYIALTYRFCTFSKMEEFMNFKERLTNSLQYVACSVEAQICDLVSCYGNITQNLSAYVAMSIEPAEDRIAWHELSDNRDLGAIIRWDPLHEVDAAEERRESFDQEVEVLQVRSLMLRLFASFIDLYHGAGLKDSPAGAETATLELLRESWTGLFQRLRVMNYKPLAQKFLVNLLPTRLHLLLDMPYERFFDDLAQLVLDLQSGSAKLAEQCKRVGDNVIATMELCVQTINESNESNGIDGLWKRRGQQQKVAASLEMLSLYAFLLSVLNDKLQVSSKTKTKKKPVENKVDGPQPISEKERSQMLQELMRQLKHKFEACDTAIRSWKAPVLPRDLSSFMSDMSLKPEVEATLIGDVSATFKDSHELMVTELRNLLKDKIRMVAK

Target ORF information:

RefSeq Version	NM_142601.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phagocyte signaling impaired (psidin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142601.2

ORF Insert Sequence:

ATGGCACAGC AGCAGGGCAT GGACTCGGCC CTGTTCGAGC GGCGACTGCG GCCGGTATAC 
GACAACCTGG AGGTGGGCAA CAATCGCAAG GCTCTGCAGG AATCCGAGAA GTTGCTACGC 
AAACATCCCA ACCTGCTCTG CGCGCGGGCA TTGAAAGGCC TCTCGCTCCT GCGACTGGGA 
CGCTATGACG AAAGCCACGG ATGCCTCCAG ACCGTTGCCG AGGAGAAGCC CACGGACGAC 
TCCACGCTGC AGGTGCTGTC CTTCTGCTAC CGCGAAATGG AACAACTGGA CAAAATTGTG 
GAGCTGTACC AGCACGCTGT GAAGCAGAAT CCGGGCAACG AGGAGCTCCT CGCCCACCTC 
TTCATCTCAC ATGTGAGGGT GGAGGACTAC AAGGCGCAGC AGGCGGTGGC CCTGCAGCTG 
TACAAGGCGC AGCCGAAAAA TGCCTACTAC TTTTGGTCGG TGATCAGCGT AGTCTTCCAG 
GGAATCCGTG GACCGGAATC GGCGCTGCCC GAGAAGCGGA AAATCTACTT GGGACTGGCG 
CAACGCATGG TCGACAAGCA CATCAAGGAG GGCAAAATGG AGACGGAACA GGAGGCCTTC 
CTCTATCTGC ACATACTTAA GCTACAAAAC AAGTACCAGG AGGCGTGGGA ATTCCTTACT 
GGTGAGCTGT GCGCGAAGCT GTACCCCGGT GCTCCAGTCA GCATGAAGTT CGAGCTGCTC 
AAGGAGCTGG GAAACTGGCG GGAATTGAAT GAGCTGCTGC AGCAGCTGCT GGACGCAGAT 
CGTGATCGTT GGGACTTCTA CAAGGAGTAT ATCCAAAGCT CGTTCGAGCT GCTAAAACTT 
ACGCCGCAGG GCGTGGAGAA CGGGGATAAG GACTCTCTGG CCAGGTGTCA GGAGTTCCTT 
CAGCGCATCA TTGACTCCTC GGAGCGCAAG AAAAGGGGAC CCTATCTGGC GCGGTTGGAG 
CTACACCAAC GCATGCGAGC GGAGCAATTG CCGGCGGAAA AACTAATCGG TGACTTTGAC 
GAGATGGTCA TTGAGTACTT CCGTCTGTTC GGCGACAAAT CGTGCTGCAC TCACGATATC 
GCCTTGTTCT TGCCTTCGAT CAGCATGAAC CAGAGGCAAG CGCTAGCCAG CAAGTTGTTG 
TTGGAGAGCG GAGTAACCTC CACGTCGCTT CCAAAAAATA AAGAGCAGTT GCAAAAACAT 
CTTTGCGCTC TGCAAATCTC TCGAATGTGC GGATCCCACA TGGATCTGCC TGCGGACCAC 
CTGCTTGCCT TCTATACGGC GCTCAAGCTG CACTACGAGC ACGGCAGGAC TACTTTCGGT 
AAGAAGTTGC TGGCTACGGA AATGGGACCA TCGGATGCAT ACGCACTGCT GGCGGCAAAT 
GTAATGTACG ATCTGAGTAG GCGGGAAAAT AAGTCGGATC ACCTGTTCGA GGCGCTGTGC 
CTGCTGCAGT ATGTTCTGCG AAACAGTACG AGTAACTTCC ACGTGAAACT GCTAAGTCTA 
AAGATCTACC ATCTGTTTGG CTGCCAAGTG GGCGCACAGG AGATGTATGA GTATCTGGAC 
ATTAAGCAGA TTCAGCTGGA CTCCATGGGC TATGTGCACT GCCAACTGTT GGCTCTTGGA 
GGTCGCTTCT CGGGCAATCG TAACGTGTAC GATGCCACCC TAAAGTTTTT TACGAACAGC 
TATAAGGAAA GGCTGGAATA CATTGCGTTA ACATATCGCT TTTGCACGTT TTCAAAAATG 
GAGGAGTTCA TGAACTTCAA GGAGCGGTTA ACCAACAGCT TGCAGTACGT GGCCTGCTCG 
GTGGAGGCGC AGATCTGTGA CTTGGTTAGT TGCTATGGCA ATATCACGCA GAATCTATCC 
GCATATGTGG CGATGAGTAT TGAGCCGGCG GAGGACCGGA TCGCTTGGCA TGAGTTGAGC 
GACAATCGCG ACTTAGGGGC CATTATCCGA TGGGATCCGC TACATGAGGT AGACGCTGCG 
GAGGAGCGCA GGGAGTCGTT CGACCAGGAA GTTGAGGTGC TTCAAGTACG TTCGCTGATG 
CTTCGACTCT TTGCCTCTTT CATCGACTTG TACCACGGCG CGGGCTTAAA GGATTCGCCA 
GCGGGAGCAG AGACGGCCAC CTTGGAGCTC CTGCGGGAGT CCTGGACGGG ATTATTCCAG 
AGATTGCGCG TGATGAACTA CAAGCCGTTG GCGCAAAAAT TTCTAGTCAA TCTGCTGCCC 
ACACGTCTTC ACCTGCTGCT CGACATGCCA TACGAACGTT TCTTTGACGA CCTTGCCCAG 
TTGGTCCTTG ACTTGCAGAG CGGGTCGGCA AAGCTGGCCG AGCAGTGCAA GCGGGTGGGT 
GACAATGTTA TTGCGACTAT GGAGCTGTGC GTGCAGACCA TCAACGAGAG CAACGAAAGC 
AACGGCATTG ACGGTCTGTG GAAGCGGCGA GGTCAGCAGC AGAAAGTGGC AGCCAGTTTA 
GAGATGCTAT CGCTGTACGC CTTCCTTCTG TCCGTGTTGA ACGACAAGCT GCAGGTAAGC 
TCAAAGACCA AGACCAAGAA GAAGCCGGTG GAAAACAAGG TGGATGGACC GCAGCCGATC 
AGCGAGAAGG AGCGCAGCCA GATGTTGCAG GAGCTGATGC GACAGCTGAA GCACAAGTTT 
GAGGCCTGCG ACACGGCCAT ACGCTCCTGG AAGGCGCCCG TTTTGCCACG CGACCTCAGC 
TCCTTTATGT CTGACATGTC GCTGAAGCCG GAGGTGGAAG CCACGCTCAT CGGCGACGTT 
TCGGCCACCT TTAAGGACTC GCACGAGCTG ATGGTCACCG AGCTGCGCAA TCTGCTGAAG 
GACAAGATAC GCATGGTGGC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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psidin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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