CG4562 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG4562
Entrez Gene ID	42362
Full Name	CG4562 gene product from transcript CG4562-RC
Synonyms	CT14766,Dme_CG4562,Dmel\CG4562
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92B4-92B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001104371.2	NP_001097841.1	uncharacterized protein, isoform B
NM_001275817.1	NP_001262746.1	uncharacterized protein, isoform D
NM_142581.2	NP_650838.1	uncharacterized protein, isoform A
NM_001275818.1	NP_001262747.1	uncharacterized protein, isoform E
NM_001275816.1	NP_001262745.1	uncharacterized protein, isoform C

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Abcc4	NP_001028508.2
Rattus norvegicus (Norway rat)	Abcc4	NP_596902.1
Gallus gallus (chicken)	ABCC4	NP_001025990.1
Drosophila melanogaster (fruit fly)	l(2)03659	NP_001260823.1
Arabidopsis thaliana (thale cress)	MRP8	NP_187916.3
Arabidopsis thaliana (thale cress)	MRP7	NP_187917.3
Pan troglodytes (chimpanzee)	ABCC4	XP_001137006.1
Drosophila melanogaster (fruit fly)	CG7627	NP_609215.3
Drosophila melanogaster (fruit fly)	CG31793	NP_609930.4
Caenorhabditis elegans (roundworm)	mrp-6	NP_508710.3
Danio rerio (zebrafish)	abcc4	NP_001007039.1
Drosophila melanogaster (fruit fly)	CG31792	NP_001260548.1
Drosophila melanogaster (fruit fly)	CG4562	NP_001262747.1
Xenopus tropicalis (tropical clawed frog)	abcc4	XP_002939329.2
Homo sapiens (human)	ABCC4	NP_005836.2
Canis lupus familiaris (dog)	ABCC4	NP_001184103.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006427	XP_316463.3
Arabidopsis thaliana (thale cress)	MRP3	NP_187915.1
Drosophila melanogaster (fruit fly)	Mrp4	NP_650086.2
Drosophila melanogaster (fruit fly)	CG9270	NP_995741.2
Oryza sativa (rice)	Os01g0173900	NP_001042159.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG4562 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG4562 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05448	NM_001104371.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG4562), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa05448	NM_001275817.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG4562), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa05446	NM_001275816.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG4562), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa05446	NM_142581.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG4562), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa05447	NM_001275818.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant E (CG4562), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG4562 ( NM_001104371.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097841.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG4562), mRNA.

Price & Availability↑

CloneID	OFa05448
Clone ID Related Accession (Same CDS sequence)	NM_001104371.2 , NM_001275817.1
Accession Version	NM_001104371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104371.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTCAA TGAAAGCCGA TGAACTGCCG GAGAATCCGC GCGAGAAGTC CAACATCTTC 
TCATCTTTGA TGTTCTGCTT TGCCATGCCC ACTTTCTTCA AGGGACGCAA AAAGACACTG 
GATGAGAATG ATTTGTACCG CGCCTTACAG GAGCACAGAT CAGATCACCT GGGCAGCAAG 
CTGAGCGCGG CGTGGGAGAA GGAGGTCGAA AAGAAGCGCA AAAAGAAGAA GACCCCCAGC 
CTGCTGAAGG CCTCGATGAA CGTGTTTGGC TGGCGCCTGG CCGGTCTAGG CCTGGTCCTC 
TTCATCTTGG AGATCGGTTT CCGAGTCACC CAGCCGCTGT TCCTGGGCGG TCTGGTGGCC 
TACTATGCGG ACGCGAGCAA CCAGGAAGGC GATAACCAGA CGAAGGCATA CCTCTACGCC 
CTGGGCGTGA TCTTGACAAG CGCCTGCAAC GTGCTCTTCA TGCATCCCTA CATGCTGGGC 
ATGTTCCACA TCGGCATGAA GGCCAGAATA GCCATGACAA GCATGATATA TCGGAAGGCG 
CTGCGATTGA GTCGAACGGC GCTGGGCGAT ACCACAATTG GTCAGGTGGT CAATCTCATC 
TCGAACGATG TGGGCCGGCT GGACGTTTCG GTCATCCACA TGAACTATTT ATGGCTGGGT 
CCGGTGGAGA TTGGCATTAT CACGTACCTC ATGTACAGGG AGATTGGTAT CTCCGCCTTC 
TTCGGCGTGG CGGTGATGTT GCTGTTTATA CCCCTGCAAG CCTATCTGGG CAAGAAGACC 
TCCGTGCTGC GATTGAAAAC GGCTCTGCGG ACGGATGAGC GAGTGCGCAT GATGAACGAG 
ATCATTTCGG GCATCCAGGT GATCAAGATG TATGCGTGGG AAATTCCCTT CAGCAAGATG 
ATCAACTATG TGCGCACCAA GGAGATGAAC GCCATCCGCA ATGTGAACTA CATCCGAGGC 
ACGCTGCAAA GCTTCATCAT GTTCGTCACG AGGATATCGG TGTTTGTGAG CTTGGTTGGA 
TTCGTGCTGC TTGGAAAGCT TCTGACCGCC GAGAAGGCCT TCGTGATCAC CGCCTACTAC 
AATATCCTGC GTAACACCAT GACCGTCTAC TTCCCCATGG GAATATCCCA GTTTGCCGAA 
CTATTGGTCT CGATCCGCCG TATCCAGACA TTCATGCTGC ACGAGGAGAC AAAGGTGCGT 
GACAAGTCGG AGGACCTGGA CGAGCAGAAG CTGGGCAAGG CGGGTCTAAT TGCTGAACCC 
ACAGTGGCCC AGACCACTGG TGTCCTGAAG CCAAGCAGTC GTCGCACCAG CGAAGCGGAG 
CACAGTATCG TCATAAGCAA GCTAAAGGCC AAATGGGATC AGAAGAACAC GGACAACACG 
TTGGATAACA TTTCGCTAAA GTTCAAGCCG CGTCAACTTG TGGCCGTCAT TGGACCAGTG 
GGCTCTGGAA AATCAAGCTT GATTCAGGCT GTGCTGGGAG AACTGAATCC GGATTCTGGT 
TCGGTCAAAG TCAACGGAAC TCTCTCGTAC GCCTCCCAGG AGCCGTGGCT CTTCACCGGC 
ACTGTTCGCC AGAACATCCT CTTCGGACTG CCCATGGATA AGCATCGCTA CCGCACTGTG 
GTCAAAAGGT GTGCCCTGGA GCGGGACTTC GAGCTGTTGC CCTATGCTGA CAAGACTATT 
GTGGGTGAGA GAGGAGCATC TCTCTCCGGA GGACAGAAGG CGCGTATAAG TTTGGCCAGA 
GCTGTTTACC GGAAGGCTGA CATCTACCTG TTGGACGATC CCCTTAGTGC CGTGGATACC 
CATGTCGGAC GTCATCTGTT CGATCAGTGT ATGCGTGGAT TCCTGCGCGA GGAAATCGTA 
CTGCTTGTGA CCCATCAACT GCAGTTCCTA GAACAGGCCG ACGTTATTGT GATCATGGAC 
AAGGGCAAGA TCAGCGCTAT GGGCACCTAC GAGTCCATGG CCAAGAGCGG TTTGGACTTC 
GCCCAAATGC TGACGGATCC CAGCAAGAAG GATGAGGGTG CCGGTGATGC CCCGGACAAG 
AAGAGCCTCT CGAGGCAGAA CAGTAAATTG CGCGACCGAC ACGGTAGCAT CTCCTCCATG 
GAATCTGCTG CCGAATCCTT GGCGGCGGAG TCACCCATGC AGACGCAGGA AGGTCGCGTC 
GAGGGGCGCA TAGGAATGAA GCTCTACAAA AAGTACTTTG GAGCGAATGG CTACGGACTG 
TTTATCGTAT TTGCGTTCTT CTGCATTGGT GCCCAGGTGC TGGCCTCTGG TGGTGATATC 
TTCTTGTCCT ACTGGGTGAA CAAAAATGGT GAAGCTGAGC GTGATACCTT TATGGCCAGA 
TTGAGACGCG CCTTCCCCGA AACCCGCATC AATGCCGATA CCGATCCTGT TGACATCTAC 
TACTTCACCG GCATCAATGT GTCTGTGATC ATCTTCTCGC TGGTGCGGAG CATGCTCTTC 
TTCTACCTGG CAATGCGCAG TTCGACGACC CTGCACAACA CCATGTTCCA AGGTGTGACC 
CGAGCGGCGA TGCATTTCTT CAACACCAAT CCCTCCGGAC GCATCCTCAA TCGCTTCTCC 
AAGGACTTGG GTCAAGTGGA CGAGATTCTT CCCTCCGTGA TGATGGACGT GATGCAGATC 
TTCCTCGCAA TCGTGGGCAT CGTAGTCGTC CTATGTATCA TTAACGTTTG GTACATCCTC 
GCCACGGTGT TCCTTGTGAT CGTTTTCTAC CTCCTGCGCG TCTTCTATCT GAGCACATCC 
CGTGACGTGA AGCGACTCGA AGCTGTCAGT TTATTAAATC AATATATTTT ATATCAACAG 
CACGCTCCCC CATTTACTCC CACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097841.1
CDS	163..3009
Translation	MQSMKADELPENPREKSNIFSSLMFCFAMPTFFKGRKKTLDENDLYRALQEHRSDHLGSKLSAAWEKEVEKKRKKKKTPSLLKASMNVFGWRLAGLGLVLFILEIGFRVTQPLFLGGLVAYYADASNQEGDNQTKAYLYALGVILTSACNVLFMHPYMLGMFHIGMKARIAMTSMIYRKALRLSRTALGDTTIGQVVNLISNDVGRLDVSVIHMNYLWLGPVEIGIITYLMYREIGISAFFGVAVMLLFIPLQAYLGKKTSVLRLKTALRTDERVRMMNEIISGIQVIKMYAWEIPFSKMINYVRTKEMNAIRNVNYIRGTLQSFIMFVTRISVFVSLVGFVLLGKLLTAEKAFVITAYYNILRNTMTVYFPMGISQFAELLVSIRRIQTFMLHEETKVRDKSEDLDEQKLGKAGLIAEPTVAQTTGVLKPSSRRTSEAEHSIVISKLKAKWDQKNTDNTLDNISLKFKPRQLVAVIGPVGSGKSSLIQAVLGELNPDSGSVKVNGTLSYASQEPWLFTGTVRQNILFGLPMDKHRYRTVVKRCALERDFELLPYADKTIVGERGASLSGGQKARISLARAVYRKADIYLLDDPLSAVDTHVGRHLFDQCMRGFLREEIVLLVTHQLQFLEQADVIVIMDKGKISAMGTYESMAKSGLDFAQMLTDPSKKDEGAGDAPDKKSLSRQNSKLRDRHGSISSMESAAESLAAESPMQTQEGRVEGRIGMKLYKKYFGANGYGLFIVFAFFCIGAQVLASGGDIFLSYWVNKNGEAERDTFMARLRRAFPETRINADTDPVDIYYFTGINVSVIIFSLVRSMLFFYLAMRSSTTLHNTMFQGVTRAAMHFFNTNPSGRILNRFSKDLGQVDEILPSVMMDVMQIFLAIVGIVVVLCIINVWYILATVFLVIVFYLLRVFYLSTSRDVKRLEAVSLLNQYILYQQHAPPFTPT

Target ORF information:

RefSeq Version	NM_001104371.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG4562), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104371.2

ORF Insert Sequence:

ATGCAGTCAA TGAAAGCCGA TGAACTGCCG GAGAATCCGC GCGAGAAGTC CAACATCTTC 
TCATCTTTGA TGTTCTGCTT TGCCATGCCC ACTTTCTTCA AGGGACGCAA AAAGACACTG 
GATGAGAATG ATTTGTACCG CGCCTTACAG GAGCACAGAT CAGATCACCT GGGCAGCAAG 
CTGAGCGCGG CGTGGGAGAA GGAGGTCGAA AAGAAGCGCA AAAAGAAGAA GACCCCCAGC 
CTGCTGAAGG CCTCGATGAA CGTGTTTGGC TGGCGCCTGG CCGGTCTAGG CCTGGTCCTC 
TTCATCTTGG AGATCGGTTT CCGAGTCACC CAGCCGCTGT TCCTGGGCGG TCTGGTGGCC 
TACTATGCGG ACGCGAGCAA CCAGGAAGGC GATAACCAGA CGAAGGCATA CCTCTACGCC 
CTGGGCGTGA TCTTGACAAG CGCCTGCAAC GTGCTCTTCA TGCATCCCTA CATGCTGGGC 
ATGTTCCACA TCGGCATGAA GGCCAGAATA GCCATGACAA GCATGATATA TCGGAAGGCG 
CTGCGATTGA GTCGAACGGC GCTGGGCGAT ACCACAATTG GTCAGGTGGT CAATCTCATC 
TCGAACGATG TGGGCCGGCT GGACGTTTCG GTCATCCACA TGAACTATTT ATGGCTGGGT 
CCGGTGGAGA TTGGCATTAT CACGTACCTC ATGTACAGGG AGATTGGTAT CTCCGCCTTC 
TTCGGCGTGG CGGTGATGTT GCTGTTTATA CCCCTGCAAG CCTATCTGGG CAAGAAGACC 
TCCGTGCTGC GATTGAAAAC GGCTCTGCGG ACGGATGAGC GAGTGCGCAT GATGAACGAG 
ATCATTTCGG GCATCCAGGT GATCAAGATG TATGCGTGGG AAATTCCCTT CAGCAAGATG 
ATCAACTATG TGCGCACCAA GGAGATGAAC GCCATCCGCA ATGTGAACTA CATCCGAGGC 
ACGCTGCAAA GCTTCATCAT GTTCGTCACG AGGATATCGG TGTTTGTGAG CTTGGTTGGA 
TTCGTGCTGC TTGGAAAGCT TCTGACCGCC GAGAAGGCCT TCGTGATCAC CGCCTACTAC 
AATATCCTGC GTAACACCAT GACCGTCTAC TTCCCCATGG GAATATCCCA GTTTGCCGAA 
CTATTGGTCT CGATCCGCCG TATCCAGACA TTCATGCTGC ACGAGGAGAC AAAGGTGCGT 
GACAAGTCGG AGGACCTGGA CGAGCAGAAG CTGGGCAAGG CGGGTCTAAT TGCTGAACCC 
ACAGTGGCCC AGACCACTGG TGTCCTGAAG CCAAGCAGTC GTCGCACCAG CGAAGCGGAG 
CACAGTATCG TCATAAGCAA GCTAAAGGCC AAATGGGATC AGAAGAACAC GGACAACACG 
TTGGATAACA TTTCGCTAAA GTTCAAGCCG CGTCAACTTG TGGCCGTCAT TGGACCAGTG 
GGCTCTGGAA AATCAAGCTT GATTCAGGCT GTGCTGGGAG AACTGAATCC GGATTCTGGT 
TCGGTCAAAG TCAACGGAAC TCTCTCGTAC GCCTCCCAGG AGCCGTGGCT CTTCACCGGC 
ACTGTTCGCC AGAACATCCT CTTCGGACTG CCCATGGATA AGCATCGCTA CCGCACTGTG 
GTCAAAAGGT GTGCCCTGGA GCGGGACTTC GAGCTGTTGC CCTATGCTGA CAAGACTATT 
GTGGGTGAGA GAGGAGCATC TCTCTCCGGA GGACAGAAGG CGCGTATAAG TTTGGCCAGA 
GCTGTTTACC GGAAGGCTGA CATCTACCTG TTGGACGATC CCCTTAGTGC CGTGGATACC 
CATGTCGGAC GTCATCTGTT CGATCAGTGT ATGCGTGGAT TCCTGCGCGA GGAAATCGTA 
CTGCTTGTGA CCCATCAACT GCAGTTCCTA GAACAGGCCG ACGTTATTGT GATCATGGAC 
AAGGGCAAGA TCAGCGCTAT GGGCACCTAC GAGTCCATGG CCAAGAGCGG TTTGGACTTC 
GCCCAAATGC TGACGGATCC CAGCAAGAAG GATGAGGGTG CCGGTGATGC CCCGGACAAG 
AAGAGCCTCT CGAGGCAGAA CAGTAAATTG CGCGACCGAC ACGGTAGCAT CTCCTCCATG 
GAATCTGCTG CCGAATCCTT GGCGGCGGAG TCACCCATGC AGACGCAGGA AGGTCGCGTC 
GAGGGGCGCA TAGGAATGAA GCTCTACAAA AAGTACTTTG GAGCGAATGG CTACGGACTG 
TTTATCGTAT TTGCGTTCTT CTGCATTGGT GCCCAGGTGC TGGCCTCTGG TGGTGATATC 
TTCTTGTCCT ACTGGGTGAA CAAAAATGGT GAAGCTGAGC GTGATACCTT TATGGCCAGA 
TTGAGACGCG CCTTCCCCGA AACCCGCATC AATGCCGATA CCGATCCTGT TGACATCTAC 
TACTTCACCG GCATCAATGT GTCTGTGATC ATCTTCTCGC TGGTGCGGAG CATGCTCTTC 
TTCTACCTGG CAATGCGCAG TTCGACGACC CTGCACAACA CCATGTTCCA AGGTGTGACC 
CGAGCGGCGA TGCATTTCTT CAACACCAAT CCCTCCGGAC GCATCCTCAA TCGCTTCTCC 
AAGGACTTGG GTCAAGTGGA CGAGATTCTT CCCTCCGTGA TGATGGACGT GATGCAGATC 
TTCCTCGCAA TCGTGGGCAT CGTAGTCGTC CTATGTATCA TTAACGTTTG GTACATCCTC 
GCCACGGTGT TCCTTGTGAT CGTTTTCTAC CTCCTGCGCG TCTTCTATCT GAGCACATCC 
CGTGACGTGA AGCGACTCGA AGCTGTCAGT TTATTAAATC AATATATTTT ATATCAACAG 
CACGCTCCCC CATTTACTCC CACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG4562 ( NM_001275817.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262746.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG4562), mRNA.

Price & Availability↑

CloneID	OFa05448
Clone ID Related Accession (Same CDS sequence)	NM_001104371.2 , NM_001275817.1
Accession Version	NM_001275817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTCAA TGAAAGCCGA TGAACTGCCG GAGAATCCGC GCGAGAAGTC CAACATCTTC 
TCATCTTTGA TGTTCTGCTT TGCCATGCCC ACTTTCTTCA AGGGACGCAA AAAGACACTG 
GATGAGAATG ATTTGTACCG CGCCTTACAG GAGCACAGAT CAGATCACCT GGGCAGCAAG 
CTGAGCGCGG CGTGGGAGAA GGAGGTCGAA AAGAAGCGCA AAAAGAAGAA GACCCCCAGC 
CTGCTGAAGG CCTCGATGAA CGTGTTTGGC TGGCGCCTGG CCGGTCTAGG CCTGGTCCTC 
TTCATCTTGG AGATCGGTTT CCGAGTCACC CAGCCGCTGT TCCTGGGCGG TCTGGTGGCC 
TACTATGCGG ACGCGAGCAA CCAGGAAGGC GATAACCAGA CGAAGGCATA CCTCTACGCC 
CTGGGCGTGA TCTTGACAAG CGCCTGCAAC GTGCTCTTCA TGCATCCCTA CATGCTGGGC 
ATGTTCCACA TCGGCATGAA GGCCAGAATA GCCATGACAA GCATGATATA TCGGAAGGCG 
CTGCGATTGA GTCGAACGGC GCTGGGCGAT ACCACAATTG GTCAGGTGGT CAATCTCATC 
TCGAACGATG TGGGCCGGCT GGACGTTTCG GTCATCCACA TGAACTATTT ATGGCTGGGT 
CCGGTGGAGA TTGGCATTAT CACGTACCTC ATGTACAGGG AGATTGGTAT CTCCGCCTTC 
TTCGGCGTGG CGGTGATGTT GCTGTTTATA CCCCTGCAAG CCTATCTGGG CAAGAAGACC 
TCCGTGCTGC GATTGAAAAC GGCTCTGCGG ACGGATGAGC GAGTGCGCAT GATGAACGAG 
ATCATTTCGG GCATCCAGGT GATCAAGATG TATGCGTGGG AAATTCCCTT CAGCAAGATG 
ATCAACTATG TGCGCACCAA GGAGATGAAC GCCATCCGCA ATGTGAACTA CATCCGAGGC 
ACGCTGCAAA GCTTCATCAT GTTCGTCACG AGGATATCGG TGTTTGTGAG CTTGGTTGGA 
TTCGTGCTGC TTGGAAAGCT TCTGACCGCC GAGAAGGCCT TCGTGATCAC CGCCTACTAC 
AATATCCTGC GTAACACCAT GACCGTCTAC TTCCCCATGG GAATATCCCA GTTTGCCGAA 
CTATTGGTCT CGATCCGCCG TATCCAGACA TTCATGCTGC ACGAGGAGAC AAAGGTGCGT 
GACAAGTCGG AGGACCTGGA CGAGCAGAAG CTGGGCAAGG CGGGTCTAAT TGCTGAACCC 
ACAGTGGCCC AGACCACTGG TGTCCTGAAG CCAAGCAGTC GTCGCACCAG CGAAGCGGAG 
CACAGTATCG TCATAAGCAA GCTAAAGGCC AAATGGGATC AGAAGAACAC GGACAACACG 
TTGGATAACA TTTCGCTAAA GTTCAAGCCG CGTCAACTTG TGGCCGTCAT TGGACCAGTG 
GGCTCTGGAA AATCAAGCTT GATTCAGGCT GTGCTGGGAG AACTGAATCC GGATTCTGGT 
TCGGTCAAAG TCAACGGAAC TCTCTCGTAC GCCTCCCAGG AGCCGTGGCT CTTCACCGGC 
ACTGTTCGCC AGAACATCCT CTTCGGACTG CCCATGGATA AGCATCGCTA CCGCACTGTG 
GTCAAAAGGT GTGCCCTGGA GCGGGACTTC GAGCTGTTGC CCTATGCTGA CAAGACTATT 
GTGGGTGAGA GAGGAGCATC TCTCTCCGGA GGACAGAAGG CGCGTATAAG TTTGGCCAGA 
GCTGTTTACC GGAAGGCTGA CATCTACCTG TTGGACGATC CCCTTAGTGC CGTGGATACC 
CATGTCGGAC GTCATCTGTT CGATCAGTGT ATGCGTGGAT TCCTGCGCGA GGAAATCGTA 
CTGCTTGTGA CCCATCAACT GCAGTTCCTA GAACAGGCCG ACGTTATTGT GATCATGGAC 
AAGGGCAAGA TCAGCGCTAT GGGCACCTAC GAGTCCATGG CCAAGAGCGG TTTGGACTTC 
GCCCAAATGC TGACGGATCC CAGCAAGAAG GATGAGGGTG CCGGTGATGC CCCGGACAAG 
AAGAGCCTCT CGAGGCAGAA CAGTAAATTG CGCGACCGAC ACGGTAGCAT CTCCTCCATG 
GAATCTGCTG CCGAATCCTT GGCGGCGGAG TCACCCATGC AGACGCAGGA AGGTCGCGTC 
GAGGGGCGCA TAGGAATGAA GCTCTACAAA AAGTACTTTG GAGCGAATGG CTACGGACTG 
TTTATCGTAT TTGCGTTCTT CTGCATTGGT GCCCAGGTGC TGGCCTCTGG TGGTGATATC 
TTCTTGTCCT ACTGGGTGAA CAAAAATGGT GAAGCTGAGC GTGATACCTT TATGGCCAGA 
TTGAGACGCG CCTTCCCCGA AACCCGCATC AATGCCGATA CCGATCCTGT TGACATCTAC 
TACTTCACCG GCATCAATGT GTCTGTGATC ATCTTCTCGC TGGTGCGGAG CATGCTCTTC 
TTCTACCTGG CAATGCGCAG TTCGACGACC CTGCACAACA CCATGTTCCA AGGTGTGACC 
CGAGCGGCGA TGCATTTCTT CAACACCAAT CCCTCCGGAC GCATCCTCAA TCGCTTCTCC 
AAGGACTTGG GTCAAGTGGA CGAGATTCTT CCCTCCGTGA TGATGGACGT GATGCAGATC 
TTCCTCGCAA TCGTGGGCAT CGTAGTCGTC CTATGTATCA TTAACGTTTG GTACATCCTC 
GCCACGGTGT TCCTTGTGAT CGTTTTCTAC CTCCTGCGCG TCTTCTATCT GAGCACATCC 
CGTGACGTGA AGCGACTCGA AGCTGTCAGT TTATTAAATC AATATATTTT ATATCAACAG 
CACGCTCCCC CATTTACTCC CACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262746.1
CDS	388..3234
Translation	MQSMKADELPENPREKSNIFSSLMFCFAMPTFFKGRKKTLDENDLYRALQEHRSDHLGSKLSAAWEKEVEKKRKKKKTPSLLKASMNVFGWRLAGLGLVLFILEIGFRVTQPLFLGGLVAYYADASNQEGDNQTKAYLYALGVILTSACNVLFMHPYMLGMFHIGMKARIAMTSMIYRKALRLSRTALGDTTIGQVVNLISNDVGRLDVSVIHMNYLWLGPVEIGIITYLMYREIGISAFFGVAVMLLFIPLQAYLGKKTSVLRLKTALRTDERVRMMNEIISGIQVIKMYAWEIPFSKMINYVRTKEMNAIRNVNYIRGTLQSFIMFVTRISVFVSLVGFVLLGKLLTAEKAFVITAYYNILRNTMTVYFPMGISQFAELLVSIRRIQTFMLHEETKVRDKSEDLDEQKLGKAGLIAEPTVAQTTGVLKPSSRRTSEAEHSIVISKLKAKWDQKNTDNTLDNISLKFKPRQLVAVIGPVGSGKSSLIQAVLGELNPDSGSVKVNGTLSYASQEPWLFTGTVRQNILFGLPMDKHRYRTVVKRCALERDFELLPYADKTIVGERGASLSGGQKARISLARAVYRKADIYLLDDPLSAVDTHVGRHLFDQCMRGFLREEIVLLVTHQLQFLEQADVIVIMDKGKISAMGTYESMAKSGLDFAQMLTDPSKKDEGAGDAPDKKSLSRQNSKLRDRHGSISSMESAAESLAAESPMQTQEGRVEGRIGMKLYKKYFGANGYGLFIVFAFFCIGAQVLASGGDIFLSYWVNKNGEAERDTFMARLRRAFPETRINADTDPVDIYYFTGINVSVIIFSLVRSMLFFYLAMRSSTTLHNTMFQGVTRAAMHFFNTNPSGRILNRFSKDLGQVDEILPSVMMDVMQIFLAIVGIVVVLCIINVWYILATVFLVIVFYLLRVFYLSTSRDVKRLEAVSLLNQYILYQQHAPPFTPT

Target ORF information:

RefSeq Version	NM_001275817.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG4562), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275817.1

ORF Insert Sequence:

ATGCAGTCAA TGAAAGCCGA TGAACTGCCG GAGAATCCGC GCGAGAAGTC CAACATCTTC 
TCATCTTTGA TGTTCTGCTT TGCCATGCCC ACTTTCTTCA AGGGACGCAA AAAGACACTG 
GATGAGAATG ATTTGTACCG CGCCTTACAG GAGCACAGAT CAGATCACCT GGGCAGCAAG 
CTGAGCGCGG CGTGGGAGAA GGAGGTCGAA AAGAAGCGCA AAAAGAAGAA GACCCCCAGC 
CTGCTGAAGG CCTCGATGAA CGTGTTTGGC TGGCGCCTGG CCGGTCTAGG CCTGGTCCTC 
TTCATCTTGG AGATCGGTTT CCGAGTCACC CAGCCGCTGT TCCTGGGCGG TCTGGTGGCC 
TACTATGCGG ACGCGAGCAA CCAGGAAGGC GATAACCAGA CGAAGGCATA CCTCTACGCC 
CTGGGCGTGA TCTTGACAAG CGCCTGCAAC GTGCTCTTCA TGCATCCCTA CATGCTGGGC 
ATGTTCCACA TCGGCATGAA GGCCAGAATA GCCATGACAA GCATGATATA TCGGAAGGCG 
CTGCGATTGA GTCGAACGGC GCTGGGCGAT ACCACAATTG GTCAGGTGGT CAATCTCATC 
TCGAACGATG TGGGCCGGCT GGACGTTTCG GTCATCCACA TGAACTATTT ATGGCTGGGT 
CCGGTGGAGA TTGGCATTAT CACGTACCTC ATGTACAGGG AGATTGGTAT CTCCGCCTTC 
TTCGGCGTGG CGGTGATGTT GCTGTTTATA CCCCTGCAAG CCTATCTGGG CAAGAAGACC 
TCCGTGCTGC GATTGAAAAC GGCTCTGCGG ACGGATGAGC GAGTGCGCAT GATGAACGAG 
ATCATTTCGG GCATCCAGGT GATCAAGATG TATGCGTGGG AAATTCCCTT CAGCAAGATG 
ATCAACTATG TGCGCACCAA GGAGATGAAC GCCATCCGCA ATGTGAACTA CATCCGAGGC 
ACGCTGCAAA GCTTCATCAT GTTCGTCACG AGGATATCGG TGTTTGTGAG CTTGGTTGGA 
TTCGTGCTGC TTGGAAAGCT TCTGACCGCC GAGAAGGCCT TCGTGATCAC CGCCTACTAC 
AATATCCTGC GTAACACCAT GACCGTCTAC TTCCCCATGG GAATATCCCA GTTTGCCGAA 
CTATTGGTCT CGATCCGCCG TATCCAGACA TTCATGCTGC ACGAGGAGAC AAAGGTGCGT 
GACAAGTCGG AGGACCTGGA CGAGCAGAAG CTGGGCAAGG CGGGTCTAAT TGCTGAACCC 
ACAGTGGCCC AGACCACTGG TGTCCTGAAG CCAAGCAGTC GTCGCACCAG CGAAGCGGAG 
CACAGTATCG TCATAAGCAA GCTAAAGGCC AAATGGGATC AGAAGAACAC GGACAACACG 
TTGGATAACA TTTCGCTAAA GTTCAAGCCG CGTCAACTTG TGGCCGTCAT TGGACCAGTG 
GGCTCTGGAA AATCAAGCTT GATTCAGGCT GTGCTGGGAG AACTGAATCC GGATTCTGGT 
TCGGTCAAAG TCAACGGAAC TCTCTCGTAC GCCTCCCAGG AGCCGTGGCT CTTCACCGGC 
ACTGTTCGCC AGAACATCCT CTTCGGACTG CCCATGGATA AGCATCGCTA CCGCACTGTG 
GTCAAAAGGT GTGCCCTGGA GCGGGACTTC GAGCTGTTGC CCTATGCTGA CAAGACTATT 
GTGGGTGAGA GAGGAGCATC TCTCTCCGGA GGACAGAAGG CGCGTATAAG TTTGGCCAGA 
GCTGTTTACC GGAAGGCTGA CATCTACCTG TTGGACGATC CCCTTAGTGC CGTGGATACC 
CATGTCGGAC GTCATCTGTT CGATCAGTGT ATGCGTGGAT TCCTGCGCGA GGAAATCGTA 
CTGCTTGTGA CCCATCAACT GCAGTTCCTA GAACAGGCCG ACGTTATTGT GATCATGGAC 
AAGGGCAAGA TCAGCGCTAT GGGCACCTAC GAGTCCATGG CCAAGAGCGG TTTGGACTTC 
GCCCAAATGC TGACGGATCC CAGCAAGAAG GATGAGGGTG CCGGTGATGC CCCGGACAAG 
AAGAGCCTCT CGAGGCAGAA CAGTAAATTG CGCGACCGAC ACGGTAGCAT CTCCTCCATG 
GAATCTGCTG CCGAATCCTT GGCGGCGGAG TCACCCATGC AGACGCAGGA AGGTCGCGTC 
GAGGGGCGCA TAGGAATGAA GCTCTACAAA AAGTACTTTG GAGCGAATGG CTACGGACTG 
TTTATCGTAT TTGCGTTCTT CTGCATTGGT GCCCAGGTGC TGGCCTCTGG TGGTGATATC 
TTCTTGTCCT ACTGGGTGAA CAAAAATGGT GAAGCTGAGC GTGATACCTT TATGGCCAGA 
TTGAGACGCG CCTTCCCCGA AACCCGCATC AATGCCGATA CCGATCCTGT TGACATCTAC 
TACTTCACCG GCATCAATGT GTCTGTGATC ATCTTCTCGC TGGTGCGGAG CATGCTCTTC 
TTCTACCTGG CAATGCGCAG TTCGACGACC CTGCACAACA CCATGTTCCA AGGTGTGACC 
CGAGCGGCGA TGCATTTCTT CAACACCAAT CCCTCCGGAC GCATCCTCAA TCGCTTCTCC 
AAGGACTTGG GTCAAGTGGA CGAGATTCTT CCCTCCGTGA TGATGGACGT GATGCAGATC 
TTCCTCGCAA TCGTGGGCAT CGTAGTCGTC CTATGTATCA TTAACGTTTG GTACATCCTC 
GCCACGGTGT TCCTTGTGAT CGTTTTCTAC CTCCTGCGCG TCTTCTATCT GAGCACATCC 
CGTGACGTGA AGCGACTCGA AGCTGTCAGT TTATTAAATC AATATATTTT ATATCAACAG 
CACGCTCCCC CATTTACTCC CACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG4562 ( NM_142581.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650838.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG4562), mRNA.

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CloneID	OFa05446
Clone ID Related Accession (Same CDS sequence)	NM_142581.2 , NM_001275816.1
Accession Version	NM_142581.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142581.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTCAA TGAAAGCCGA TGAACTGCCG GAGAATCCGC GCGAGAAGTC CAACATCTTC 
TCATCTTTGA TGTTCTGCTT TGCCATGCCC ACTTTCTTCA AGGGACGCAA AAAGACACTG 
GATGAGAATG ATTTGTACCG CGCCTTACAG GAGCACAGAT CAGATCACCT GGGCAGCAAG 
CTGAGCGCGG CGTGGGAGAA GGAGGTCGAA AAGAAGCGCA AAAAGAAGAA GACCCCCAGC 
CTGCTGAAGG CCTCGATGAA CGTGTTTGGC TGGCGCCTGG CCGGTCTAGG CCTGGTCCTC 
TTCATCTTGG AGATCGGTTT CCGAGTCACC CAGCCGCTGT TCCTGGGCGG TCTGGTGGCC 
TACTATGCGG ACGCGAGCAA CCAGGAAGGC GATAACCAGA CGAAGGCATA CCTCTACGCC 
CTGGGCGTGA TCTTGACAAG CGCCTGCAAC GTGCTCTTCA TGCATCCCTA CATGCTGGGC 
ATGTTCCACA TCGGCATGAA GGCCAGAATA GCCATGACAA GCATGATATA TCGGAAGGCG 
CTGCGATTGA GTCGAACGGC GCTGGGCGAT ACCACAATTG GTCAGGTGGT CAATCTCATC 
TCGAACGATG TGGGCCGGCT GGACGTTTCG GTCATCCACA TGAACTATTT ATGGCTGGGT 
CCGGTGGAGA TTGGCATTAT CACGTACCTC ATGTACAGGG AGATTGGTAT CTCCGCCTTC 
TTCGGCGTGG CGGTGATGTT GCTGTTTATA CCCCTGCAAG CCTATCTGGG CAAGAAGACC 
TCCGTGCTGC GATTGAAAAC GGCTCTGCGG ACGGATGAGC GAGTGCGCAT GATGAACGAG 
ATCATTTCGG GCATCCAGGT GATCAAGATG TATGCGTGGG AAATTCCCTT CAGCAAGATG 
ATCAACTATG TGCGCACCAA GGAGATGAAC GCCATCCGCA ATGTGAACTA CATCCGAGGC 
ACGCTGCAAA GCTTCATCAT GTTCGTCACG AGGATATCGG TGTTTGTGAG CTTGGTTGGA 
TTCGTGCTGC TTGGAAAGCT TCTGACCGCC GAGAAGGCCT TCGTGATCAC CGCCTACTAC 
AATATCCTGC GTAACACCAT GACCGTCTAC TTCCCCATGG GAATATCCCA GTTTGCCGAA 
CTATTGGTCT CGATCCGCCG TATCCAGACA TTCATGCTGC ACGAGGAGAC AAAGGTGCGT 
GACAAGTCGG AGGACCTGGA CGAGCAGAAG CTGGGCAAGG CGGGTCTAAT TGCTGAACCC 
ACAGTGGCCC AGACCACTGG TGTCCTGAAG CCAAGCAGTC GTCGCACCAG CGAAGCGGAG 
CACAGTATCG TCATAAGCAA GCTAAAGGCC AAATGGGATC AGAAGAACAC GGACAACACG 
TTGGATAACA TTTCGCTAAA GTTCAAGCCG CGTCAACTTG TGGCCGTCAT TGGACCAGTG 
GGCTCTGGAA AATCAAGCTT GATTCAGGCT GTGCTGGGAG AACTGAATCC GGATTCTGGT 
TCGGTCAAAG TCAACGGAAC TCTCTCGTAC GCCTCCCAGG AGCCGTGGCT CTTCACCGGC 
ACTGTTCGCC AGAACATCCT CTTCGGACTG CCCATGGATA AGCATCGCTA CCGCACTGTG 
GTCAAAAGGT GTGCCCTGGA GCGGGACTTC GAGCTGTTGC CCTATGCTGA CAAGACTATT 
GTGGGTGAGA GAGGAGCATC TCTCTCCGGA GGACAGAAGG CGCGTATAAG TTTGGCCAGA 
GCTGTTTACC GGAAGGCTGA CATCTACCTG TTGGACGATC CCCTTAGTGC CGTGGATACC 
CATGTCGGAC GTCATCTGTT CGATCAGTGT ATGCGTGGAT TCCTGCGCGA GGAAATCGTA 
CTGCTTGTGA CCCATCAACT GCAGTTCCTA GAACAGGCCG ACGTTATTGT GATCATGGAC 
AAGGGCAAGA TCAGCGCTAT GGGCACCTAC GAGTCCATGG CCAAGAGCGG TTTGGACTTC 
GCCCAAATGC TGACGGATCC CAGCAAGAAG GATGAGGGTG CCGGTGATGC CCCGGACAAG 
AAGAGCCTCT CGAGGCAGAA CAGTAAATTG CGCGACCGAC ACGGTAGCAT CTCCTCCATG 
GAATCTGCTG CCGAATCCTT GGCGGCGGAG TCACCCATGC AGACGCAGGA AGGTCGCGTC 
GAGGGGCGCA TAGGAATGAA GCTCTACAAA AAGTACTTTG GAGCGAATGG CTACGGACTG 
TTTATCGTAT TTGCGTTCTT CTGCATTGGT GCCCAGGTGC TGGCCTCTGG TGGTGATATC 
TTCTTGTCCT ACTGGGTGAA CAAAAATGGT GAAGCTGAGC GTGATACCTT TATGGCCAGA 
TTGAGACGCG CCTTCCCCGA AACCCGCATC AATGCCGATA CCGATCCTGT TGACATCTAC 
TACTTCACCG GCATCAATGT GTCTGTGATC ATCTTCTCGC TGGTGCGGAG CATGCTCTTC 
TTCTACCTGG CAATGCGCAG TTCGACGACC CTGCACAACA CCATGTTCCA AGGTGTGACC 
CGAGCGGCGA TGCATTTCTT CAACACCAAT CCCTCCGGAC GCATCCTCAA TCGCTTCTCC 
AAGGACTTGG GTCAAGTGGA CGAGATTCTT CCCTCCGTGA TGATGGACGT GATGCAGATC 
TTCCTCGCAA TCGTGGGCAT CGTAGTCGTC CTATGTATCA TTAACGTTTG GTACATCCTC 
GCCACGGTGT TCCTTGTGAT CGTTTTCTAC CTCCTGCGCG TCTTCTATCT GAGCACATCC 
CGTGACGTGA AGCGACTCGA AGCTGTCACA CGCTCCCCCA TTTACTCCCA CTTGAGTGCT 
TCCCTTAATG GCCTGGCCAC CATCCGAGCA TTTGGAGCGC AGAAGGAACT GATTGCCGAA 
TTCGACAACT ACCAGGATAT GCACAGCTCT GGCTACTATA TGTTCCTTGC CACGAGTCGA 
GCCTTTGGAT ACTGGCTCGA CTGCGTTTGT GTTGTCTACA TCGCTGTAAT CACATTGAGC 
TTCTTCCTCT TCTCCCCGGA GAACGGAGGT GACGTCGGTC TTGCTATTAC CCAAGCCATG 
GGCATGACCG GTATGGTCCA GTGGGGAATG AGACAATCGG CCGAGCTGGA AAACACAATG 
ACGGCAGTGG AGAGAGTGGT GGAATATGAG GATCTTGAAC CGGAGGGTGA CTTCGAATCC 
AAGCCAAATA AAAAGCCGCC AAAGGATTGG CCAGAGGATG GAAAGATCGT GTTCGACGAC 
CTCTCGCTGA AGTACTTCCC CGACAAGGCG GCCGACTACG TGCTCCGATC CCTGAACATC 
GCTATCGAGG GTTGCGAAAA GGTGGGCATT GTGGGACGAA CTGGTGCCGG CAAATCTTCG 
CTGATCAACG CTCTGTTCCG TCTGTCCTAC AACGAGGGAG CCATCCTGAT CGACAGGCGC 
GACACCAACG ACCTGGGACT GCACGATCTG CGCAGCAAGA TCTCCATTAT TCCTCAGGAA 
CCGGTGCTGT TCTCGGGCAC CATGCGTTAC AATCTGGATC CCTTCGACGA GTATAGCGAC 
GCCAAGCTGT GGGAGTCTCT GGAGGAGGTT AAGCTTAAGC AAGTGGTGGC TGATCTTCCC 
AGTGGTTTGC AGAGCAAGAT ATCCGAAGGC GGCACCAACT TCAGCGTGGG ACAGCGACAG 
CTGGTGTGCC TAGCTAGGGC CATTCTCCGA GAGAATCGCA TTTTGGTGAT GGACGAGGCA 
ACGGCTAACG TCGATCCGCA GACGGATGCT CTTATCCAGA CGACGATTAG GAACAAATTC 
AAGGACTGCA CGGTGCTCAC GATTGCCCAT CGATTGCATA CGGTTATGGA CTCGGACAAG 
GTGCTAGTAA TGGACGCAGG AAAGGCGGTG GAGTTCGGAT CTCCGTTCGA GCTGCTCACA 
ACCAGCGAGA AGAAGGTCTT CCACAGCATG GTGAAGCAGA CGGGAGACTC CACTTTCGAT 
GCCCTGCTGA AGGTTGCCCA AAAGGTGGGA TTGCGTTTTA AGATTCAATG TAACTTGTCC 
ACATGCCTAA GCTCTTTGTC TTCCTTTCAG GCCCATGATG ACAACCTGCG GCTCAAGAAG 
GATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650838.1
CDS	146..4234
Translation	MQSMKADELPENPREKSNIFSSLMFCFAMPTFFKGRKKTLDENDLYRALQEHRSDHLGSKLSAAWEKEVEKKRKKKKTPSLLKASMNVFGWRLAGLGLVLFILEIGFRVTQPLFLGGLVAYYADASNQEGDNQTKAYLYALGVILTSACNVLFMHPYMLGMFHIGMKARIAMTSMIYRKALRLSRTALGDTTIGQVVNLISNDVGRLDVSVIHMNYLWLGPVEIGIITYLMYREIGISAFFGVAVMLLFIPLQAYLGKKTSVLRLKTALRTDERVRMMNEIISGIQVIKMYAWEIPFSKMINYVRTKEMNAIRNVNYIRGTLQSFIMFVTRISVFVSLVGFVLLGKLLTAEKAFVITAYYNILRNTMTVYFPMGISQFAELLVSIRRIQTFMLHEETKVRDKSEDLDEQKLGKAGLIAEPTVAQTTGVLKPSSRRTSEAEHSIVISKLKAKWDQKNTDNTLDNISLKFKPRQLVAVIGPVGSGKSSLIQAVLGELNPDSGSVKVNGTLSYASQEPWLFTGTVRQNILFGLPMDKHRYRTVVKRCALERDFELLPYADKTIVGERGASLSGGQKARISLARAVYRKADIYLLDDPLSAVDTHVGRHLFDQCMRGFLREEIVLLVTHQLQFLEQADVIVIMDKGKISAMGTYESMAKSGLDFAQMLTDPSKKDEGAGDAPDKKSLSRQNSKLRDRHGSISSMESAAESLAAESPMQTQEGRVEGRIGMKLYKKYFGANGYGLFIVFAFFCIGAQVLASGGDIFLSYWVNKNGEAERDTFMARLRRAFPETRINADTDPVDIYYFTGINVSVIIFSLVRSMLFFYLAMRSSTTLHNTMFQGVTRAAMHFFNTNPSGRILNRFSKDLGQVDEILPSVMMDVMQIFLAIVGIVVVLCIINVWYILATVFLVIVFYLLRVFYLSTSRDVKRLEAVTRSPIYSHLSASLNGLATIRAFGAQKELIAEFDNYQDMHSSGYYMFLATSRAFGYWLDCVCVVYIAVITLSFFLFSPENGGDVGLAITQAMGMTGMVQWGMRQSAELENTMTAVERVVEYEDLEPEGDFESKPNKKPPKDWPEDGKIVFDDLSLKYFPDKAADYVLRSLNIAIEGCEKVGIVGRTGAGKSSLINALFRLSYNEGAILIDRRDTNDLGLHDLRSKISIIPQEPVLFSGTMRYNLDPFDEYSDAKLWESLEEVKLKQVVADLPSGLQSKISEGGTNFSVGQRQLVCLARAILRENRILVMDEATANVDPQTDALIQTTIRNKFKDCTVLTIAHRLHTVMDSDKVLVMDAGKAVEFGSPFELLTTSEKKVFHSMVKQTGDSTFDALLKVAQKVGLRFKIQCNLSTCLSSLSSFQAHDDNLRLKKDS

Target ORF information:

RefSeq Version	NM_142581.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG4562), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142581.2

ORF Insert Sequence:

ATGCAGTCAA TGAAAGCCGA TGAACTGCCG GAGAATCCGC GCGAGAAGTC CAACATCTTC 
TCATCTTTGA TGTTCTGCTT TGCCATGCCC ACTTTCTTCA AGGGACGCAA AAAGACACTG 
GATGAGAATG ATTTGTACCG CGCCTTACAG GAGCACAGAT CAGATCACCT GGGCAGCAAG 
CTGAGCGCGG CGTGGGAGAA GGAGGTCGAA AAGAAGCGCA AAAAGAAGAA GACCCCCAGC 
CTGCTGAAGG CCTCGATGAA CGTGTTTGGC TGGCGCCTGG CCGGTCTAGG CCTGGTCCTC 
TTCATCTTGG AGATCGGTTT CCGAGTCACC CAGCCGCTGT TCCTGGGCGG TCTGGTGGCC 
TACTATGCGG ACGCGAGCAA CCAGGAAGGC GATAACCAGA CGAAGGCATA CCTCTACGCC 
CTGGGCGTGA TCTTGACAAG CGCCTGCAAC GTGCTCTTCA TGCATCCCTA CATGCTGGGC 
ATGTTCCACA TCGGCATGAA GGCCAGAATA GCCATGACAA GCATGATATA TCGGAAGGCG 
CTGCGATTGA GTCGAACGGC GCTGGGCGAT ACCACAATTG GTCAGGTGGT CAATCTCATC 
TCGAACGATG TGGGCCGGCT GGACGTTTCG GTCATCCACA TGAACTATTT ATGGCTGGGT 
CCGGTGGAGA TTGGCATTAT CACGTACCTC ATGTACAGGG AGATTGGTAT CTCCGCCTTC 
TTCGGCGTGG CGGTGATGTT GCTGTTTATA CCCCTGCAAG CCTATCTGGG CAAGAAGACC 
TCCGTGCTGC GATTGAAAAC GGCTCTGCGG ACGGATGAGC GAGTGCGCAT GATGAACGAG 
ATCATTTCGG GCATCCAGGT GATCAAGATG TATGCGTGGG AAATTCCCTT CAGCAAGATG 
ATCAACTATG TGCGCACCAA GGAGATGAAC GCCATCCGCA ATGTGAACTA CATCCGAGGC 
ACGCTGCAAA GCTTCATCAT GTTCGTCACG AGGATATCGG TGTTTGTGAG CTTGGTTGGA 
TTCGTGCTGC TTGGAAAGCT TCTGACCGCC GAGAAGGCCT TCGTGATCAC CGCCTACTAC 
AATATCCTGC GTAACACCAT GACCGTCTAC TTCCCCATGG GAATATCCCA GTTTGCCGAA 
CTATTGGTCT CGATCCGCCG TATCCAGACA TTCATGCTGC ACGAGGAGAC AAAGGTGCGT 
GACAAGTCGG AGGACCTGGA CGAGCAGAAG CTGGGCAAGG CGGGTCTAAT TGCTGAACCC 
ACAGTGGCCC AGACCACTGG TGTCCTGAAG CCAAGCAGTC GTCGCACCAG CGAAGCGGAG 
CACAGTATCG TCATAAGCAA GCTAAAGGCC AAATGGGATC AGAAGAACAC GGACAACACG 
TTGGATAACA TTTCGCTAAA GTTCAAGCCG CGTCAACTTG TGGCCGTCAT TGGACCAGTG 
GGCTCTGGAA AATCAAGCTT GATTCAGGCT GTGCTGGGAG AACTGAATCC GGATTCTGGT 
TCGGTCAAAG TCAACGGAAC TCTCTCGTAC GCCTCCCAGG AGCCGTGGCT CTTCACCGGC 
ACTGTTCGCC AGAACATCCT CTTCGGACTG CCCATGGATA AGCATCGCTA CCGCACTGTG 
GTCAAAAGGT GTGCCCTGGA GCGGGACTTC GAGCTGTTGC CCTATGCTGA CAAGACTATT 
GTGGGTGAGA GAGGAGCATC TCTCTCCGGA GGACAGAAGG CGCGTATAAG TTTGGCCAGA 
GCTGTTTACC GGAAGGCTGA CATCTACCTG TTGGACGATC CCCTTAGTGC CGTGGATACC 
CATGTCGGAC GTCATCTGTT CGATCAGTGT ATGCGTGGAT TCCTGCGCGA GGAAATCGTA 
CTGCTTGTGA CCCATCAACT GCAGTTCCTA GAACAGGCCG ACGTTATTGT GATCATGGAC 
AAGGGCAAGA TCAGCGCTAT GGGCACCTAC GAGTCCATGG CCAAGAGCGG TTTGGACTTC 
GCCCAAATGC TGACGGATCC CAGCAAGAAG GATGAGGGTG CCGGTGATGC CCCGGACAAG 
AAGAGCCTCT CGAGGCAGAA CAGTAAATTG CGCGACCGAC ACGGTAGCAT CTCCTCCATG 
GAATCTGCTG CCGAATCCTT GGCGGCGGAG TCACCCATGC AGACGCAGGA AGGTCGCGTC 
GAGGGGCGCA TAGGAATGAA GCTCTACAAA AAGTACTTTG GAGCGAATGG CTACGGACTG 
TTTATCGTAT TTGCGTTCTT CTGCATTGGT GCCCAGGTGC TGGCCTCTGG TGGTGATATC 
TTCTTGTCCT ACTGGGTGAA CAAAAATGGT GAAGCTGAGC GTGATACCTT TATGGCCAGA 
TTGAGACGCG CCTTCCCCGA AACCCGCATC AATGCCGATA CCGATCCTGT TGACATCTAC 
TACTTCACCG GCATCAATGT GTCTGTGATC ATCTTCTCGC TGGTGCGGAG CATGCTCTTC 
TTCTACCTGG CAATGCGCAG TTCGACGACC CTGCACAACA CCATGTTCCA AGGTGTGACC 
CGAGCGGCGA TGCATTTCTT CAACACCAAT CCCTCCGGAC GCATCCTCAA TCGCTTCTCC 
AAGGACTTGG GTCAAGTGGA CGAGATTCTT CCCTCCGTGA TGATGGACGT GATGCAGATC 
TTCCTCGCAA TCGTGGGCAT CGTAGTCGTC CTATGTATCA TTAACGTTTG GTACATCCTC 
GCCACGGTGT TCCTTGTGAT CGTTTTCTAC CTCCTGCGCG TCTTCTATCT GAGCACATCC 
CGTGACGTGA AGCGACTCGA AGCTGTCACA CGCTCCCCCA TTTACTCCCA CTTGAGTGCT 
TCCCTTAATG GCCTGGCCAC CATCCGAGCA TTTGGAGCGC AGAAGGAACT GATTGCCGAA 
TTCGACAACT ACCAGGATAT GCACAGCTCT GGCTACTATA TGTTCCTTGC CACGAGTCGA 
GCCTTTGGAT ACTGGCTCGA CTGCGTTTGT GTTGTCTACA TCGCTGTAAT CACATTGAGC 
TTCTTCCTCT TCTCCCCGGA GAACGGAGGT GACGTCGGTC TTGCTATTAC CCAAGCCATG 
GGCATGACCG GTATGGTCCA GTGGGGAATG AGACAATCGG CCGAGCTGGA AAACACAATG 
ACGGCAGTGG AGAGAGTGGT GGAATATGAG GATCTTGAAC CGGAGGGTGA CTTCGAATCC 
AAGCCAAATA AAAAGCCGCC AAAGGATTGG CCAGAGGATG GAAAGATCGT GTTCGACGAC 
CTCTCGCTGA AGTACTTCCC CGACAAGGCG GCCGACTACG TGCTCCGATC CCTGAACATC 
GCTATCGAGG GTTGCGAAAA GGTGGGCATT GTGGGACGAA CTGGTGCCGG CAAATCTTCG 
CTGATCAACG CTCTGTTCCG TCTGTCCTAC AACGAGGGAG CCATCCTGAT CGACAGGCGC 
GACACCAACG ACCTGGGACT GCACGATCTG CGCAGCAAGA TCTCCATTAT TCCTCAGGAA 
CCGGTGCTGT TCTCGGGCAC CATGCGTTAC AATCTGGATC CCTTCGACGA GTATAGCGAC 
GCCAAGCTGT GGGAGTCTCT GGAGGAGGTT AAGCTTAAGC AAGTGGTGGC TGATCTTCCC 
AGTGGTTTGC AGAGCAAGAT ATCCGAAGGC GGCACCAACT TCAGCGTGGG ACAGCGACAG 
CTGGTGTGCC TAGCTAGGGC CATTCTCCGA GAGAATCGCA TTTTGGTGAT GGACGAGGCA 
ACGGCTAACG TCGATCCGCA GACGGATGCT CTTATCCAGA CGACGATTAG GAACAAATTC 
AAGGACTGCA CGGTGCTCAC GATTGCCCAT CGATTGCATA CGGTTATGGA CTCGGACAAG 
GTGCTAGTAA TGGACGCAGG AAAGGCGGTG GAGTTCGGAT CTCCGTTCGA GCTGCTCACA 
ACCAGCGAGA AGAAGGTCTT CCACAGCATG GTGAAGCAGA CGGGAGACTC CACTTTCGAT 
GCCCTGCTGA AGGTTGCCCA AAAGGTGGGA TTGCGTTTTA AGATTCAATG TAACTTGTCC 
ACATGCCTAA GCTCTTTGTC TTCCTTTCAG GCCCATGATG ACAACCTGCG GCTCAAGAAG 
GATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG4562 ( NM_001275816.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262745.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG4562), mRNA.

Price & Availability↑

CloneID	OFa05446
Clone ID Related Accession (Same CDS sequence)	NM_142581.2 , NM_001275816.1
Accession Version	NM_001275816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTCAA TGAAAGCCGA TGAACTGCCG GAGAATCCGC GCGAGAAGTC CAACATCTTC 
TCATCTTTGA TGTTCTGCTT TGCCATGCCC ACTTTCTTCA AGGGACGCAA AAAGACACTG 
GATGAGAATG ATTTGTACCG CGCCTTACAG GAGCACAGAT CAGATCACCT GGGCAGCAAG 
CTGAGCGCGG CGTGGGAGAA GGAGGTCGAA AAGAAGCGCA AAAAGAAGAA GACCCCCAGC 
CTGCTGAAGG CCTCGATGAA CGTGTTTGGC TGGCGCCTGG CCGGTCTAGG CCTGGTCCTC 
TTCATCTTGG AGATCGGTTT CCGAGTCACC CAGCCGCTGT TCCTGGGCGG TCTGGTGGCC 
TACTATGCGG ACGCGAGCAA CCAGGAAGGC GATAACCAGA CGAAGGCATA CCTCTACGCC 
CTGGGCGTGA TCTTGACAAG CGCCTGCAAC GTGCTCTTCA TGCATCCCTA CATGCTGGGC 
ATGTTCCACA TCGGCATGAA GGCCAGAATA GCCATGACAA GCATGATATA TCGGAAGGCG 
CTGCGATTGA GTCGAACGGC GCTGGGCGAT ACCACAATTG GTCAGGTGGT CAATCTCATC 
TCGAACGATG TGGGCCGGCT GGACGTTTCG GTCATCCACA TGAACTATTT ATGGCTGGGT 
CCGGTGGAGA TTGGCATTAT CACGTACCTC ATGTACAGGG AGATTGGTAT CTCCGCCTTC 
TTCGGCGTGG CGGTGATGTT GCTGTTTATA CCCCTGCAAG CCTATCTGGG CAAGAAGACC 
TCCGTGCTGC GATTGAAAAC GGCTCTGCGG ACGGATGAGC GAGTGCGCAT GATGAACGAG 
ATCATTTCGG GCATCCAGGT GATCAAGATG TATGCGTGGG AAATTCCCTT CAGCAAGATG 
ATCAACTATG TGCGCACCAA GGAGATGAAC GCCATCCGCA ATGTGAACTA CATCCGAGGC 
ACGCTGCAAA GCTTCATCAT GTTCGTCACG AGGATATCGG TGTTTGTGAG CTTGGTTGGA 
TTCGTGCTGC TTGGAAAGCT TCTGACCGCC GAGAAGGCCT TCGTGATCAC CGCCTACTAC 
AATATCCTGC GTAACACCAT GACCGTCTAC TTCCCCATGG GAATATCCCA GTTTGCCGAA 
CTATTGGTCT CGATCCGCCG TATCCAGACA TTCATGCTGC ACGAGGAGAC AAAGGTGCGT 
GACAAGTCGG AGGACCTGGA CGAGCAGAAG CTGGGCAAGG CGGGTCTAAT TGCTGAACCC 
ACAGTGGCCC AGACCACTGG TGTCCTGAAG CCAAGCAGTC GTCGCACCAG CGAAGCGGAG 
CACAGTATCG TCATAAGCAA GCTAAAGGCC AAATGGGATC AGAAGAACAC GGACAACACG 
TTGGATAACA TTTCGCTAAA GTTCAAGCCG CGTCAACTTG TGGCCGTCAT TGGACCAGTG 
GGCTCTGGAA AATCAAGCTT GATTCAGGCT GTGCTGGGAG AACTGAATCC GGATTCTGGT 
TCGGTCAAAG TCAACGGAAC TCTCTCGTAC GCCTCCCAGG AGCCGTGGCT CTTCACCGGC 
ACTGTTCGCC AGAACATCCT CTTCGGACTG CCCATGGATA AGCATCGCTA CCGCACTGTG 
GTCAAAAGGT GTGCCCTGGA GCGGGACTTC GAGCTGTTGC CCTATGCTGA CAAGACTATT 
GTGGGTGAGA GAGGAGCATC TCTCTCCGGA GGACAGAAGG CGCGTATAAG TTTGGCCAGA 
GCTGTTTACC GGAAGGCTGA CATCTACCTG TTGGACGATC CCCTTAGTGC CGTGGATACC 
CATGTCGGAC GTCATCTGTT CGATCAGTGT ATGCGTGGAT TCCTGCGCGA GGAAATCGTA 
CTGCTTGTGA CCCATCAACT GCAGTTCCTA GAACAGGCCG ACGTTATTGT GATCATGGAC 
AAGGGCAAGA TCAGCGCTAT GGGCACCTAC GAGTCCATGG CCAAGAGCGG TTTGGACTTC 
GCCCAAATGC TGACGGATCC CAGCAAGAAG GATGAGGGTG CCGGTGATGC CCCGGACAAG 
AAGAGCCTCT CGAGGCAGAA CAGTAAATTG CGCGACCGAC ACGGTAGCAT CTCCTCCATG 
GAATCTGCTG CCGAATCCTT GGCGGCGGAG TCACCCATGC AGACGCAGGA AGGTCGCGTC 
GAGGGGCGCA TAGGAATGAA GCTCTACAAA AAGTACTTTG GAGCGAATGG CTACGGACTG 
TTTATCGTAT TTGCGTTCTT CTGCATTGGT GCCCAGGTGC TGGCCTCTGG TGGTGATATC 
TTCTTGTCCT ACTGGGTGAA CAAAAATGGT GAAGCTGAGC GTGATACCTT TATGGCCAGA 
TTGAGACGCG CCTTCCCCGA AACCCGCATC AATGCCGATA CCGATCCTGT TGACATCTAC 
TACTTCACCG GCATCAATGT GTCTGTGATC ATCTTCTCGC TGGTGCGGAG CATGCTCTTC 
TTCTACCTGG CAATGCGCAG TTCGACGACC CTGCACAACA CCATGTTCCA AGGTGTGACC 
CGAGCGGCGA TGCATTTCTT CAACACCAAT CCCTCCGGAC GCATCCTCAA TCGCTTCTCC 
AAGGACTTGG GTCAAGTGGA CGAGATTCTT CCCTCCGTGA TGATGGACGT GATGCAGATC 
TTCCTCGCAA TCGTGGGCAT CGTAGTCGTC CTATGTATCA TTAACGTTTG GTACATCCTC 
GCCACGGTGT TCCTTGTGAT CGTTTTCTAC CTCCTGCGCG TCTTCTATCT GAGCACATCC 
CGTGACGTGA AGCGACTCGA AGCTGTCACA CGCTCCCCCA TTTACTCCCA CTTGAGTGCT 
TCCCTTAATG GCCTGGCCAC CATCCGAGCA TTTGGAGCGC AGAAGGAACT GATTGCCGAA 
TTCGACAACT ACCAGGATAT GCACAGCTCT GGCTACTATA TGTTCCTTGC CACGAGTCGA 
GCCTTTGGAT ACTGGCTCGA CTGCGTTTGT GTTGTCTACA TCGCTGTAAT CACATTGAGC 
TTCTTCCTCT TCTCCCCGGA GAACGGAGGT GACGTCGGTC TTGCTATTAC CCAAGCCATG 
GGCATGACCG GTATGGTCCA GTGGGGAATG AGACAATCGG CCGAGCTGGA AAACACAATG 
ACGGCAGTGG AGAGAGTGGT GGAATATGAG GATCTTGAAC CGGAGGGTGA CTTCGAATCC 
AAGCCAAATA AAAAGCCGCC AAAGGATTGG CCAGAGGATG GAAAGATCGT GTTCGACGAC 
CTCTCGCTGA AGTACTTCCC CGACAAGGCG GCCGACTACG TGCTCCGATC CCTGAACATC 
GCTATCGAGG GTTGCGAAAA GGTGGGCATT GTGGGACGAA CTGGTGCCGG CAAATCTTCG 
CTGATCAACG CTCTGTTCCG TCTGTCCTAC AACGAGGGAG CCATCCTGAT CGACAGGCGC 
GACACCAACG ACCTGGGACT GCACGATCTG CGCAGCAAGA TCTCCATTAT TCCTCAGGAA 
CCGGTGCTGT TCTCGGGCAC CATGCGTTAC AATCTGGATC CCTTCGACGA GTATAGCGAC 
GCCAAGCTGT GGGAGTCTCT GGAGGAGGTT AAGCTTAAGC AAGTGGTGGC TGATCTTCCC 
AGTGGTTTGC AGAGCAAGAT ATCCGAAGGC GGCACCAACT TCAGCGTGGG ACAGCGACAG 
CTGGTGTGCC TAGCTAGGGC CATTCTCCGA GAGAATCGCA TTTTGGTGAT GGACGAGGCA 
ACGGCTAACG TCGATCCGCA GACGGATGCT CTTATCCAGA CGACGATTAG GAACAAATTC 
AAGGACTGCA CGGTGCTCAC GATTGCCCAT CGATTGCATA CGGTTATGGA CTCGGACAAG 
GTGCTAGTAA TGGACGCAGG AAAGGCGGTG GAGTTCGGAT CTCCGTTCGA GCTGCTCACA 
ACCAGCGAGA AGAAGGTCTT CCACAGCATG GTGAAGCAGA CGGGAGACTC CACTTTCGAT 
GCCCTGCTGA AGGTTGCCCA AAAGGTGGGA TTGCGTTTTA AGATTCAATG TAACTTGTCC 
ACATGCCTAA GCTCTTTGTC TTCCTTTCAG GCCCATGATG ACAACCTGCG GCTCAAGAAG 
GATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262745.1
CDS	371..4459
Translation	MQSMKADELPENPREKSNIFSSLMFCFAMPTFFKGRKKTLDENDLYRALQEHRSDHLGSKLSAAWEKEVEKKRKKKKTPSLLKASMNVFGWRLAGLGLVLFILEIGFRVTQPLFLGGLVAYYADASNQEGDNQTKAYLYALGVILTSACNVLFMHPYMLGMFHIGMKARIAMTSMIYRKALRLSRTALGDTTIGQVVNLISNDVGRLDVSVIHMNYLWLGPVEIGIITYLMYREIGISAFFGVAVMLLFIPLQAYLGKKTSVLRLKTALRTDERVRMMNEIISGIQVIKMYAWEIPFSKMINYVRTKEMNAIRNVNYIRGTLQSFIMFVTRISVFVSLVGFVLLGKLLTAEKAFVITAYYNILRNTMTVYFPMGISQFAELLVSIRRIQTFMLHEETKVRDKSEDLDEQKLGKAGLIAEPTVAQTTGVLKPSSRRTSEAEHSIVISKLKAKWDQKNTDNTLDNISLKFKPRQLVAVIGPVGSGKSSLIQAVLGELNPDSGSVKVNGTLSYASQEPWLFTGTVRQNILFGLPMDKHRYRTVVKRCALERDFELLPYADKTIVGERGASLSGGQKARISLARAVYRKADIYLLDDPLSAVDTHVGRHLFDQCMRGFLREEIVLLVTHQLQFLEQADVIVIMDKGKISAMGTYESMAKSGLDFAQMLTDPSKKDEGAGDAPDKKSLSRQNSKLRDRHGSISSMESAAESLAAESPMQTQEGRVEGRIGMKLYKKYFGANGYGLFIVFAFFCIGAQVLASGGDIFLSYWVNKNGEAERDTFMARLRRAFPETRINADTDPVDIYYFTGINVSVIIFSLVRSMLFFYLAMRSSTTLHNTMFQGVTRAAMHFFNTNPSGRILNRFSKDLGQVDEILPSVMMDVMQIFLAIVGIVVVLCIINVWYILATVFLVIVFYLLRVFYLSTSRDVKRLEAVTRSPIYSHLSASLNGLATIRAFGAQKELIAEFDNYQDMHSSGYYMFLATSRAFGYWLDCVCVVYIAVITLSFFLFSPENGGDVGLAITQAMGMTGMVQWGMRQSAELENTMTAVERVVEYEDLEPEGDFESKPNKKPPKDWPEDGKIVFDDLSLKYFPDKAADYVLRSLNIAIEGCEKVGIVGRTGAGKSSLINALFRLSYNEGAILIDRRDTNDLGLHDLRSKISIIPQEPVLFSGTMRYNLDPFDEYSDAKLWESLEEVKLKQVVADLPSGLQSKISEGGTNFSVGQRQLVCLARAILRENRILVMDEATANVDPQTDALIQTTIRNKFKDCTVLTIAHRLHTVMDSDKVLVMDAGKAVEFGSPFELLTTSEKKVFHSMVKQTGDSTFDALLKVAQKVGLRFKIQCNLSTCLSSLSSFQAHDDNLRLKKDS

Target ORF information:

RefSeq Version	NM_001275816.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG4562), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275816.1

ORF Insert Sequence:

ATGCAGTCAA TGAAAGCCGA TGAACTGCCG GAGAATCCGC GCGAGAAGTC CAACATCTTC 
TCATCTTTGA TGTTCTGCTT TGCCATGCCC ACTTTCTTCA AGGGACGCAA AAAGACACTG 
GATGAGAATG ATTTGTACCG CGCCTTACAG GAGCACAGAT CAGATCACCT GGGCAGCAAG 
CTGAGCGCGG CGTGGGAGAA GGAGGTCGAA AAGAAGCGCA AAAAGAAGAA GACCCCCAGC 
CTGCTGAAGG CCTCGATGAA CGTGTTTGGC TGGCGCCTGG CCGGTCTAGG CCTGGTCCTC 
TTCATCTTGG AGATCGGTTT CCGAGTCACC CAGCCGCTGT TCCTGGGCGG TCTGGTGGCC 
TACTATGCGG ACGCGAGCAA CCAGGAAGGC GATAACCAGA CGAAGGCATA CCTCTACGCC 
CTGGGCGTGA TCTTGACAAG CGCCTGCAAC GTGCTCTTCA TGCATCCCTA CATGCTGGGC 
ATGTTCCACA TCGGCATGAA GGCCAGAATA GCCATGACAA GCATGATATA TCGGAAGGCG 
CTGCGATTGA GTCGAACGGC GCTGGGCGAT ACCACAATTG GTCAGGTGGT CAATCTCATC 
TCGAACGATG TGGGCCGGCT GGACGTTTCG GTCATCCACA TGAACTATTT ATGGCTGGGT 
CCGGTGGAGA TTGGCATTAT CACGTACCTC ATGTACAGGG AGATTGGTAT CTCCGCCTTC 
TTCGGCGTGG CGGTGATGTT GCTGTTTATA CCCCTGCAAG CCTATCTGGG CAAGAAGACC 
TCCGTGCTGC GATTGAAAAC GGCTCTGCGG ACGGATGAGC GAGTGCGCAT GATGAACGAG 
ATCATTTCGG GCATCCAGGT GATCAAGATG TATGCGTGGG AAATTCCCTT CAGCAAGATG 
ATCAACTATG TGCGCACCAA GGAGATGAAC GCCATCCGCA ATGTGAACTA CATCCGAGGC 
ACGCTGCAAA GCTTCATCAT GTTCGTCACG AGGATATCGG TGTTTGTGAG CTTGGTTGGA 
TTCGTGCTGC TTGGAAAGCT TCTGACCGCC GAGAAGGCCT TCGTGATCAC CGCCTACTAC 
AATATCCTGC GTAACACCAT GACCGTCTAC TTCCCCATGG GAATATCCCA GTTTGCCGAA 
CTATTGGTCT CGATCCGCCG TATCCAGACA TTCATGCTGC ACGAGGAGAC AAAGGTGCGT 
GACAAGTCGG AGGACCTGGA CGAGCAGAAG CTGGGCAAGG CGGGTCTAAT TGCTGAACCC 
ACAGTGGCCC AGACCACTGG TGTCCTGAAG CCAAGCAGTC GTCGCACCAG CGAAGCGGAG 
CACAGTATCG TCATAAGCAA GCTAAAGGCC AAATGGGATC AGAAGAACAC GGACAACACG 
TTGGATAACA TTTCGCTAAA GTTCAAGCCG CGTCAACTTG TGGCCGTCAT TGGACCAGTG 
GGCTCTGGAA AATCAAGCTT GATTCAGGCT GTGCTGGGAG AACTGAATCC GGATTCTGGT 
TCGGTCAAAG TCAACGGAAC TCTCTCGTAC GCCTCCCAGG AGCCGTGGCT CTTCACCGGC 
ACTGTTCGCC AGAACATCCT CTTCGGACTG CCCATGGATA AGCATCGCTA CCGCACTGTG 
GTCAAAAGGT GTGCCCTGGA GCGGGACTTC GAGCTGTTGC CCTATGCTGA CAAGACTATT 
GTGGGTGAGA GAGGAGCATC TCTCTCCGGA GGACAGAAGG CGCGTATAAG TTTGGCCAGA 
GCTGTTTACC GGAAGGCTGA CATCTACCTG TTGGACGATC CCCTTAGTGC CGTGGATACC 
CATGTCGGAC GTCATCTGTT CGATCAGTGT ATGCGTGGAT TCCTGCGCGA GGAAATCGTA 
CTGCTTGTGA CCCATCAACT GCAGTTCCTA GAACAGGCCG ACGTTATTGT GATCATGGAC 
AAGGGCAAGA TCAGCGCTAT GGGCACCTAC GAGTCCATGG CCAAGAGCGG TTTGGACTTC 
GCCCAAATGC TGACGGATCC CAGCAAGAAG GATGAGGGTG CCGGTGATGC CCCGGACAAG 
AAGAGCCTCT CGAGGCAGAA CAGTAAATTG CGCGACCGAC ACGGTAGCAT CTCCTCCATG 
GAATCTGCTG CCGAATCCTT GGCGGCGGAG TCACCCATGC AGACGCAGGA AGGTCGCGTC 
GAGGGGCGCA TAGGAATGAA GCTCTACAAA AAGTACTTTG GAGCGAATGG CTACGGACTG 
TTTATCGTAT TTGCGTTCTT CTGCATTGGT GCCCAGGTGC TGGCCTCTGG TGGTGATATC 
TTCTTGTCCT ACTGGGTGAA CAAAAATGGT GAAGCTGAGC GTGATACCTT TATGGCCAGA 
TTGAGACGCG CCTTCCCCGA AACCCGCATC AATGCCGATA CCGATCCTGT TGACATCTAC 
TACTTCACCG GCATCAATGT GTCTGTGATC ATCTTCTCGC TGGTGCGGAG CATGCTCTTC 
TTCTACCTGG CAATGCGCAG TTCGACGACC CTGCACAACA CCATGTTCCA AGGTGTGACC 
CGAGCGGCGA TGCATTTCTT CAACACCAAT CCCTCCGGAC GCATCCTCAA TCGCTTCTCC 
AAGGACTTGG GTCAAGTGGA CGAGATTCTT CCCTCCGTGA TGATGGACGT GATGCAGATC 
TTCCTCGCAA TCGTGGGCAT CGTAGTCGTC CTATGTATCA TTAACGTTTG GTACATCCTC 
GCCACGGTGT TCCTTGTGAT CGTTTTCTAC CTCCTGCGCG TCTTCTATCT GAGCACATCC 
CGTGACGTGA AGCGACTCGA AGCTGTCACA CGCTCCCCCA TTTACTCCCA CTTGAGTGCT 
TCCCTTAATG GCCTGGCCAC CATCCGAGCA TTTGGAGCGC AGAAGGAACT GATTGCCGAA 
TTCGACAACT ACCAGGATAT GCACAGCTCT GGCTACTATA TGTTCCTTGC CACGAGTCGA 
GCCTTTGGAT ACTGGCTCGA CTGCGTTTGT GTTGTCTACA TCGCTGTAAT CACATTGAGC 
TTCTTCCTCT TCTCCCCGGA GAACGGAGGT GACGTCGGTC TTGCTATTAC CCAAGCCATG 
GGCATGACCG GTATGGTCCA GTGGGGAATG AGACAATCGG CCGAGCTGGA AAACACAATG 
ACGGCAGTGG AGAGAGTGGT GGAATATGAG GATCTTGAAC CGGAGGGTGA CTTCGAATCC 
AAGCCAAATA AAAAGCCGCC AAAGGATTGG CCAGAGGATG GAAAGATCGT GTTCGACGAC 
CTCTCGCTGA AGTACTTCCC CGACAAGGCG GCCGACTACG TGCTCCGATC CCTGAACATC 
GCTATCGAGG GTTGCGAAAA GGTGGGCATT GTGGGACGAA CTGGTGCCGG CAAATCTTCG 
CTGATCAACG CTCTGTTCCG TCTGTCCTAC AACGAGGGAG CCATCCTGAT CGACAGGCGC 
GACACCAACG ACCTGGGACT GCACGATCTG CGCAGCAAGA TCTCCATTAT TCCTCAGGAA 
CCGGTGCTGT TCTCGGGCAC CATGCGTTAC AATCTGGATC CCTTCGACGA GTATAGCGAC 
GCCAAGCTGT GGGAGTCTCT GGAGGAGGTT AAGCTTAAGC AAGTGGTGGC TGATCTTCCC 
AGTGGTTTGC AGAGCAAGAT ATCCGAAGGC GGCACCAACT TCAGCGTGGG ACAGCGACAG 
CTGGTGTGCC TAGCTAGGGC CATTCTCCGA GAGAATCGCA TTTTGGTGAT GGACGAGGCA 
ACGGCTAACG TCGATCCGCA GACGGATGCT CTTATCCAGA CGACGATTAG GAACAAATTC 
AAGGACTGCA CGGTGCTCAC GATTGCCCAT CGATTGCATA CGGTTATGGA CTCGGACAAG 
GTGCTAGTAA TGGACGCAGG AAAGGCGGTG GAGTTCGGAT CTCCGTTCGA GCTGCTCACA 
ACCAGCGAGA AGAAGGTCTT CCACAGCATG GTGAAGCAGA CGGGAGACTC CACTTTCGAT 
GCCCTGCTGA AGGTTGCCCA AAAGGTGGGA TTGCGTTTTA AGATTCAATG TAACTTGTCC 
ACATGCCTAA GCTCTTTGTC TTCCTTTCAG GCCCATGATG ACAACCTGCG GCTCAAGAAG 
GATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG4562 ( NM_001275818.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262747.1 Drosophila melanogaster uncharacterized protein, transcript variant E (CG4562), mRNA.

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CloneID	OFa05447
Clone ID Related Accession (Same CDS sequence)	NM_001275818.1
Accession Version	NM_001275818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTCAA TGAAAGCCGA TGAACTGCCG GAGAATCCGC GCGAGAAGTC CAACATCTTC 
TCATCTTTGA TGTTCTGCTT TGCCATGCCC ACTTTCTTCA AGGGACGCAA AAAGACACTG 
GATGAGAATG ATTTGTACCG CGCCTTACAG GAGCACAGAT CAGATCACCT GGGCAGCAAG 
CTGAGCGCGG CGTGGGAGAA GGAGGTCGAA AAGAAGCGCA AAAAGAAGAA GACCCCCAGC 
CTGCTGAAGG CCTCGATGAA CGTGTTTGGC TGGCGCCTGG CCGGTCTAGG CCTGGTCCTC 
TTCATCTTGG AGATCGGTTT CCGAGTCACC CAGCCGCTGT TCCTGGGCGG TCTGGTGGCC 
TACTATGCGG ACGCGAGCAA CCAGGAAGGC GATAACCAGA CGAAGGCATA CCTCTACGCC 
CTGGGCGTGA TCTTGACAAG CGCCTGCAAC GTGCTCTTCA TGCATCCCTA CATGCTGGGC 
ATGTTCCACA TCGGCATGAA GGCCAGAATA GCCATGACAA GCATGATATA TCGGAAGGCG 
CTGCGATTGA GTCGAACGGC GCTGGGCGAT ACCACAATTG GTCAGGTGGT CAATCTCATC 
TCGAACGATG TGGGCCGGCT GGACGTTTCG GTCATCCACA TGAACTATTT ATGGCTGGGT 
CCGGTGGAGA TTGGCATTAT CACGTACCTC ATGTACAGGG AGATTGGTAT CTCCGCCTTC 
TTCGGCGTGG CGGTGATGTT GCTGTTTATA CCCCTGCAAG CCTATCTGGG CAAGAAGACC 
TCCGTGCTGC GATTGAAAAC GGCTCTGCGG ACGGATGAGC GAGTGCGCAT GATGAACGAG 
ATCATTTCGG GCATCCAGGT GATCAAGATG TATGCGTGGG AAATTCCCTT CAGCAAGATG 
ATCAACTATG TGCGCACCAA GGAGATGAAC GCCATCCGCA ATGTGAACTA CATCCGAGGC 
ACGCTGCAAA GCTTCATCAT GTTCGTCACG AGGATATCGG TGTTTGTGAG CTTGGTTGGA 
TTCGTGCTGC TTGGAAAGCT TCTGACCGCC GAGAAGGCCT TCGTGATCAC CGCCTACTAC 
AATATCCTGC GTAACACCAT GACCGTCTAC TTCCCCATGG GAATATCCCA GTTTGCCGAA 
CTATTGGTCT CGATCCGCCG TATCCAGACA TTCATGCTGC ACGAGGAGAC AAAGGTGCGT 
GACAAGTCGG AGGACCTGGA CGAGCAGAAG CTGGGCAAGG CGGGTCTAAT TGCTGAACCC 
ACAGTGGCCC AGACCACTGG TGTCCTGAAG CCAAGCAGTC GTCGCACCAG CGAAGCGGAG 
CACAGTATCG TCATAAGCAA GCTAAAGGCC AAATGGGATC AGAAGAACAC GGACAACACG 
TTGGATAACA TTTCGCTAAA GTTCAAGCCG CGTCAACTTG TGGCCGTCAT TGGACCAGTG 
GGCTCTGGAA AATCAAGCTT GATTCAGGCT GTGCTGGGAG AACTGAATCC GGATTCTGGT 
TCGGTCAAAG TCAACGGAAC TCTCTCGTAC GCCTCCCAGG AGCCGTGGCT CTTCACCGGC 
ACTGTTCGCC AGAACATCCT CTTCGGACTG CCCATGGATA AGCATCGCTA CCGCACTGTG 
GTCAAAAGGT GTGCCCTGGA GCGGGACTTC GAGCTGTTGC CCTATGCTGA CAAGACTATT 
GTGGGTGAGA GAGGAGCATC TCTCTCCGGA GGACAGAAGG CGCGTATAAG TTTGGCCAGA 
GCTGTTTACC GGAAGGCTGA CATCTACCTG TTGGACGATC CCCTTAGTGC CGTGGATACC 
CATGTCGGAC GTCATCTGTT CGATCAGTGT ATGCGTGGAT TCCTGCGCGA GGAAATCGTA 
CTGCTTGTGA CCCATCAACT GCAGTTCCTA GAACAGGCCG ACGTTATTGT GATCATGGAC 
AAGGGCAAGA TCAGCGCTAT GGGCACCTAC GAGTCCATGG CCAAGAGCGG TTTGGACTTC 
GCCCAAATGC TGACGGATCC CAGCAAGAAG GATGAGGGTG CCGGTGATGC CCCGGACAAG 
AAGAGCCTCT CGAGGCAGAA CAGTAAATTG CGCGACCGAC ACGGTAGCAT CTCCTCCATG 
GAATCTGCTG CCGAATCCTT GGCGGCGGAG TCACCCATGC AGACGCAGGA AGGTCGCGTC 
GAGGGGCGCA TAGGAATGAA GCTCTACAAA AAGTACTTTG GAGCGAATGG CTACGGACTG 
TTTATCGTAT TTGCGTTCTT CTGCATTGGT GCCCAGGTGC TGGCCTCTGG TGGTGATATC 
TTCTTGTCCT ACTGGGTGAA CAAAAATGGT GAAGCTGAGC GTGATACCTT TATGGCCAGA 
TTGAGACGCG CCTTCCCCGA AACCCGCATC AATGCCGATA CCGATCCTGT TGACATCTAC 
TACTTCACCG GCATCAATGT GTCTGTGATC ATCTTCTCGC TGGTGCGGAG CATGCTCTTC 
TTCTACCTGG CAATGCGCAG TTCGACGACC CTGCACAACA CCATGTTCCA AGGTGTGACC 
CGAGCGGCGA TGCATTTCTT CAACACCAAT CCCTCCGGAC GCATCCTCAA TCGCTTCTCC 
AAGGACTTGG GTCAAGTGGA CGAGATTCTT CCCTCCGTGA TGATGGACGT GATGCAGATC 
TTCCTCGCAA TCGTGGGCAT CGTAGTCGTC CTATGTATCA TTAACGTTTG GTACATCCTC 
GCCACGGTGT TCCTTGTGAT CGTTTTCTAC CTCCTGCGCG TCTTCTATCT GAGCACATCC 
CGTGACGTGA AGCGACTCGA AGCTGTCACA CGCTCCCCCA TTTACTCCCA CTTGAGTGCT 
TCCCTTAATG GCCTGGCCAC CATCCGAGCA TTTGGAGCGC AGAAGGAACT GATTGCCGAA 
TTCGACAACT ACCAGGATAT GCACAGCTCT GGCTACTATA TGTTCCTTGC CACGAGTCGA 
GCCTTTGGAT ACTGGCTCGA CTGCGTTTGT GTTGTCTACA TCGCTGTAAT CACATTGAGC 
TTCTTCCTCT TCTCCCCGGA GAACGGAGGT GACGTCGGTC TTGCTATTAC CCAAGCCATG 
GGCATGACCG GTATGGTCCA GTGGGGAATG AGACAATCGG CCGAGCTGGA AAACACAATG 
ACGGCAGTGG AGAGAGTGGT GGAATATGAG GATCTTGAAC CGGAGGGTGA CTTCGAATCC 
AAGCCAAATA AAAAGCCGCC AAAGGATTGG CCAGAGGATG GAAAGATCGT GTTCGACGAC 
CTCTCGCTGA AGTACTTCCC CGACAAGGCG GCCGACTACG TGCTCCGATC CCTGAACATC 
GCTATCGAGG GTTGCGAAAA GGTGGGCATT GTGGGACGAA CTGGTGCCGG CAAATCTTCG 
CTGATCAACG CTCTGTTCCG TCTGTCCTAC AACGAGGGAG CCATCCTGAT CGACAGGCGC 
GACACCAACG ACCTGGGACT GCACGATCTG CGCAGCAAGA TCTCCATTAT TCCTCAGGAA 
CCGGTGCTGT TCTCGGGCAC CATGCGTTAC AATCTGGATC CCTTCGACGA GTATAGCGAC 
GCCAAGCTGT GGGAGTCTCT GGAGGAGGTT AAGCTTAAGC AAGTGGTGGC TGATCTTCCC 
AGTGGTTTGC AGAGCAAGAT ATCCGAAGGC GGCACCAACT TCAGCGTGGG ACAGCGACAG 
CTGGTGTGCC TAGCTAGGGC CATTCTCCGA GAGAATCGCA TTTTGGTGAT GGACGAGGCA 
ACGGCTAACG TCGATCCGCA GACGGATGCT CTTATCCAGA CGACGATTAG GAACAAATTC 
AAGGACTGCA CGGTGCTCAC GATTGCCCAT CGATTGCATA CGGTTATGGA CTCGGACAAG 
GTGCTAGTAA TGGACGCAGG AAAGGCGGTG GAGTTCGGAT CTCCGTTCGA GCTGCTCACA 
ACCAGCGAGA AGAAGGTCTT CCACAGCATG GTGAAGCAGA CGGGAGACTC CACTTTCGAT 
GCCCTGCTGA AGGTTGCCCA AAAGGTGGGA TTGCGTTTTA AGATTCAATG TAACTTGTCC 
ACATGCCTAA GCTCTTTGTC TTCCTTTCAG GCCCATGATG ACAACCTGCG GCTCAAGAAG 
GATTCTTGAC ATTTCGCCGT TCCGAAGGCT TTTAAAGACT ACATACTTAA GATACTGGAA 
CCAATTACGA ATCATCCGGC GGCCAATGCG GGCATTCAGA AGACCTTCCT CTTTGCGGAG 
GCCTCCAAGC AGAAGTTGTT AGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262747.1
CDS	146..4372
Translation	MQSMKADELPENPREKSNIFSSLMFCFAMPTFFKGRKKTLDENDLYRALQEHRSDHLGSKLSAAWEKEVEKKRKKKKTPSLLKASMNVFGWRLAGLGLVLFILEIGFRVTQPLFLGGLVAYYADASNQEGDNQTKAYLYALGVILTSACNVLFMHPYMLGMFHIGMKARIAMTSMIYRKALRLSRTALGDTTIGQVVNLISNDVGRLDVSVIHMNYLWLGPVEIGIITYLMYREIGISAFFGVAVMLLFIPLQAYLGKKTSVLRLKTALRTDERVRMMNEIISGIQVIKMYAWEIPFSKMINYVRTKEMNAIRNVNYIRGTLQSFIMFVTRISVFVSLVGFVLLGKLLTAEKAFVITAYYNILRNTMTVYFPMGISQFAELLVSIRRIQTFMLHEETKVRDKSEDLDEQKLGKAGLIAEPTVAQTTGVLKPSSRRTSEAEHSIVISKLKAKWDQKNTDNTLDNISLKFKPRQLVAVIGPVGSGKSSLIQAVLGELNPDSGSVKVNGTLSYASQEPWLFTGTVRQNILFGLPMDKHRYRTVVKRCALERDFELLPYADKTIVGERGASLSGGQKARISLARAVYRKADIYLLDDPLSAVDTHVGRHLFDQCMRGFLREEIVLLVTHQLQFLEQADVIVIMDKGKISAMGTYESMAKSGLDFAQMLTDPSKKDEGAGDAPDKKSLSRQNSKLRDRHGSISSMESAAESLAAESPMQTQEGRVEGRIGMKLYKKYFGANGYGLFIVFAFFCIGAQVLASGGDIFLSYWVNKNGEAERDTFMARLRRAFPETRINADTDPVDIYYFTGINVSVIIFSLVRSMLFFYLAMRSSTTLHNTMFQGVTRAAMHFFNTNPSGRILNRFSKDLGQVDEILPSVMMDVMQIFLAIVGIVVVLCIINVWYILATVFLVIVFYLLRVFYLSTSRDVKRLEAVTRSPIYSHLSASLNGLATIRAFGAQKELIAEFDNYQDMHSSGYYMFLATSRAFGYWLDCVCVVYIAVITLSFFLFSPENGGDVGLAITQAMGMTGMVQWGMRQSAELENTMTAVERVVEYEDLEPEGDFESKPNKKPPKDWPEDGKIVFDDLSLKYFPDKAADYVLRSLNIAIEGCEKVGIVGRTGAGKSSLINALFRLSYNEGAILIDRRDTNDLGLHDLRSKISIIPQEPVLFSGTMRYNLDPFDEYSDAKLWESLEEVKLKQVVADLPSGLQSKISEGGTNFSVGQRQLVCLARAILRENRILVMDEATANVDPQTDALIQTTIRNKFKDCTVLTIAHRLHTVMDSDKVLVMDAGKAVEFGSPFELLTTSEKKVFHSMVKQTGDSTFDALLKVAQKVGLRFKIQCNLSTCLSSLSSFQAHDDNLRLKKDS*HFAVPKAFKDYILKILEPITNHPAANAGIQKTFLFAEASKQKLLG

Target ORF information:

RefSeq Version	NM_001275818.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant E (CG4562), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275818.1

ORF Insert Sequence:

ATGCAGTCAA TGAAAGCCGA TGAACTGCCG GAGAATCCGC GCGAGAAGTC CAACATCTTC 
TCATCTTTGA TGTTCTGCTT TGCCATGCCC ACTTTCTTCA AGGGACGCAA AAAGACACTG 
GATGAGAATG ATTTGTACCG CGCCTTACAG GAGCACAGAT CAGATCACCT GGGCAGCAAG 
CTGAGCGCGG CGTGGGAGAA GGAGGTCGAA AAGAAGCGCA AAAAGAAGAA GACCCCCAGC 
CTGCTGAAGG CCTCGATGAA CGTGTTTGGC TGGCGCCTGG CCGGTCTAGG CCTGGTCCTC 
TTCATCTTGG AGATCGGTTT CCGAGTCACC CAGCCGCTGT TCCTGGGCGG TCTGGTGGCC 
TACTATGCGG ACGCGAGCAA CCAGGAAGGC GATAACCAGA CGAAGGCATA CCTCTACGCC 
CTGGGCGTGA TCTTGACAAG CGCCTGCAAC GTGCTCTTCA TGCATCCCTA CATGCTGGGC 
ATGTTCCACA TCGGCATGAA GGCCAGAATA GCCATGACAA GCATGATATA TCGGAAGGCG 
CTGCGATTGA GTCGAACGGC GCTGGGCGAT ACCACAATTG GTCAGGTGGT CAATCTCATC 
TCGAACGATG TGGGCCGGCT GGACGTTTCG GTCATCCACA TGAACTATTT ATGGCTGGGT 
CCGGTGGAGA TTGGCATTAT CACGTACCTC ATGTACAGGG AGATTGGTAT CTCCGCCTTC 
TTCGGCGTGG CGGTGATGTT GCTGTTTATA CCCCTGCAAG CCTATCTGGG CAAGAAGACC 
TCCGTGCTGC GATTGAAAAC GGCTCTGCGG ACGGATGAGC GAGTGCGCAT GATGAACGAG 
ATCATTTCGG GCATCCAGGT GATCAAGATG TATGCGTGGG AAATTCCCTT CAGCAAGATG 
ATCAACTATG TGCGCACCAA GGAGATGAAC GCCATCCGCA ATGTGAACTA CATCCGAGGC 
ACGCTGCAAA GCTTCATCAT GTTCGTCACG AGGATATCGG TGTTTGTGAG CTTGGTTGGA 
TTCGTGCTGC TTGGAAAGCT TCTGACCGCC GAGAAGGCCT TCGTGATCAC CGCCTACTAC 
AATATCCTGC GTAACACCAT GACCGTCTAC TTCCCCATGG GAATATCCCA GTTTGCCGAA 
CTATTGGTCT CGATCCGCCG TATCCAGACA TTCATGCTGC ACGAGGAGAC AAAGGTGCGT 
GACAAGTCGG AGGACCTGGA CGAGCAGAAG CTGGGCAAGG CGGGTCTAAT TGCTGAACCC 
ACAGTGGCCC AGACCACTGG TGTCCTGAAG CCAAGCAGTC GTCGCACCAG CGAAGCGGAG 
CACAGTATCG TCATAAGCAA GCTAAAGGCC AAATGGGATC AGAAGAACAC GGACAACACG 
TTGGATAACA TTTCGCTAAA GTTCAAGCCG CGTCAACTTG TGGCCGTCAT TGGACCAGTG 
GGCTCTGGAA AATCAAGCTT GATTCAGGCT GTGCTGGGAG AACTGAATCC GGATTCTGGT 
TCGGTCAAAG TCAACGGAAC TCTCTCGTAC GCCTCCCAGG AGCCGTGGCT CTTCACCGGC 
ACTGTTCGCC AGAACATCCT CTTCGGACTG CCCATGGATA AGCATCGCTA CCGCACTGTG 
GTCAAAAGGT GTGCCCTGGA GCGGGACTTC GAGCTGTTGC CCTATGCTGA CAAGACTATT 
GTGGGTGAGA GAGGAGCATC TCTCTCCGGA GGACAGAAGG CGCGTATAAG TTTGGCCAGA 
GCTGTTTACC GGAAGGCTGA CATCTACCTG TTGGACGATC CCCTTAGTGC CGTGGATACC 
CATGTCGGAC GTCATCTGTT CGATCAGTGT ATGCGTGGAT TCCTGCGCGA GGAAATCGTA 
CTGCTTGTGA CCCATCAACT GCAGTTCCTA GAACAGGCCG ACGTTATTGT GATCATGGAC 
AAGGGCAAGA TCAGCGCTAT GGGCACCTAC GAGTCCATGG CCAAGAGCGG TTTGGACTTC 
GCCCAAATGC TGACGGATCC CAGCAAGAAG GATGAGGGTG CCGGTGATGC CCCGGACAAG 
AAGAGCCTCT CGAGGCAGAA CAGTAAATTG CGCGACCGAC ACGGTAGCAT CTCCTCCATG 
GAATCTGCTG CCGAATCCTT GGCGGCGGAG TCACCCATGC AGACGCAGGA AGGTCGCGTC 
GAGGGGCGCA TAGGAATGAA GCTCTACAAA AAGTACTTTG GAGCGAATGG CTACGGACTG 
TTTATCGTAT TTGCGTTCTT CTGCATTGGT GCCCAGGTGC TGGCCTCTGG TGGTGATATC 
TTCTTGTCCT ACTGGGTGAA CAAAAATGGT GAAGCTGAGC GTGATACCTT TATGGCCAGA 
TTGAGACGCG CCTTCCCCGA AACCCGCATC AATGCCGATA CCGATCCTGT TGACATCTAC 
TACTTCACCG GCATCAATGT GTCTGTGATC ATCTTCTCGC TGGTGCGGAG CATGCTCTTC 
TTCTACCTGG CAATGCGCAG TTCGACGACC CTGCACAACA CCATGTTCCA AGGTGTGACC 
CGAGCGGCGA TGCATTTCTT CAACACCAAT CCCTCCGGAC GCATCCTCAA TCGCTTCTCC 
AAGGACTTGG GTCAAGTGGA CGAGATTCTT CCCTCCGTGA TGATGGACGT GATGCAGATC 
TTCCTCGCAA TCGTGGGCAT CGTAGTCGTC CTATGTATCA TTAACGTTTG GTACATCCTC 
GCCACGGTGT TCCTTGTGAT CGTTTTCTAC CTCCTGCGCG TCTTCTATCT GAGCACATCC 
CGTGACGTGA AGCGACTCGA AGCTGTCACA CGCTCCCCCA TTTACTCCCA CTTGAGTGCT 
TCCCTTAATG GCCTGGCCAC CATCCGAGCA TTTGGAGCGC AGAAGGAACT GATTGCCGAA 
TTCGACAACT ACCAGGATAT GCACAGCTCT GGCTACTATA TGTTCCTTGC CACGAGTCGA 
GCCTTTGGAT ACTGGCTCGA CTGCGTTTGT GTTGTCTACA TCGCTGTAAT CACATTGAGC 
TTCTTCCTCT TCTCCCCGGA GAACGGAGGT GACGTCGGTC TTGCTATTAC CCAAGCCATG 
GGCATGACCG GTATGGTCCA GTGGGGAATG AGACAATCGG CCGAGCTGGA AAACACAATG 
ACGGCAGTGG AGAGAGTGGT GGAATATGAG GATCTTGAAC CGGAGGGTGA CTTCGAATCC 
AAGCCAAATA AAAAGCCGCC AAAGGATTGG CCAGAGGATG GAAAGATCGT GTTCGACGAC 
CTCTCGCTGA AGTACTTCCC CGACAAGGCG GCCGACTACG TGCTCCGATC CCTGAACATC 
GCTATCGAGG GTTGCGAAAA GGTGGGCATT GTGGGACGAA CTGGTGCCGG CAAATCTTCG 
CTGATCAACG CTCTGTTCCG TCTGTCCTAC AACGAGGGAG CCATCCTGAT CGACAGGCGC 
GACACCAACG ACCTGGGACT GCACGATCTG CGCAGCAAGA TCTCCATTAT TCCTCAGGAA 
CCGGTGCTGT TCTCGGGCAC CATGCGTTAC AATCTGGATC CCTTCGACGA GTATAGCGAC 
GCCAAGCTGT GGGAGTCTCT GGAGGAGGTT AAGCTTAAGC AAGTGGTGGC TGATCTTCCC 
AGTGGTTTGC AGAGCAAGAT ATCCGAAGGC GGCACCAACT TCAGCGTGGG ACAGCGACAG 
CTGGTGTGCC TAGCTAGGGC CATTCTCCGA GAGAATCGCA TTTTGGTGAT GGACGAGGCA 
ACGGCTAACG TCGATCCGCA GACGGATGCT CTTATCCAGA CGACGATTAG GAACAAATTC 
AAGGACTGCA CGGTGCTCAC GATTGCCCAT CGATTGCATA CGGTTATGGA CTCGGACAAG 
GTGCTAGTAA TGGACGCAGG AAAGGCGGTG GAGTTCGGAT CTCCGTTCGA GCTGCTCACA 
ACCAGCGAGA AGAAGGTCTT CCACAGCATG GTGAAGCAGA CGGGAGACTC CACTTTCGAT 
GCCCTGCTGA AGGTTGCCCA AAAGGTGGGA TTGCGTTTTA AGATTCAATG TAACTTGTCC 
ACATGCCTAA GCTCTTTGTC TTCCTTTCAG GCCCATGATG ACAACCTGCG GCTCAAGAAG 
GATTCTTGAC ATTTCGCCGT TCCGAAGGCT TTTAAAGACT ACATACTTAA GATACTGGAA 
CCAATTACGA ATCATCCGGC GGCCAATGCG GGCATTCAGA AGACCTTCCT CTTTGCGGAG 
GCCTCCAAGC AGAAGTTGTT AGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG4562 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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