Ppcs cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppcs
Entrez Gene ID	42295
Full Name	Phosphopantothenoylcysteine synthetase
Synonyms	BcDNA:GH03502,CG5629,Dmel\CG5629,GH03502,PPCS,anon-WO0118547.401,dPPCS
General protein information	Preferred Names Phosphopantothenoylcysteine synthetase Names CG5629 gene product from transcript CG5629-RA 4'-phosphopantothenoylcysteine synthetase CG5629-PA CG5629-PC CG5629-PD Ppcs-PA Ppcs-PC Ppcs-PD phosphopantothenoylcysteine synthetase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91F3-91F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_142527.3	NP_650784.3	phosphopantothenoylcysteine synthetase, isoform C
NM_169848.3	NP_732401.1	phosphopantothenoylcysteine synthetase, isoform A
NM_001316504.1	NP_001303433.1	phosphopantothenoylcysteine synthetase, isoform D

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPCS	XP_532539.1
Mus musculus (house mouse)	Ppcs	NP_080770.2
Caenorhabditis elegans (roundworm)	Y71H2AM.6	NP_497616.1
Eremothecium gossypii	AGOS_AGR391W	NP_987057.2
Magnaporthe oryzae (rice blast fungus)	MGG_17982	XP_003721034.1
Neurospora crassa	NCU09090	XP_964907.1
Arabidopsis thaliana (thale cress)	AT5G02080	NP_195828.1
Oryza sativa (rice)	Os02g0575200	NP_001047211.1
Homo sapiens (human)	PPCS	NP_078940.2
Gallus gallus (chicken)	PPCS	XP_004947590.1
Saccharomyces cerevisiae (baker's yeast)	CAB2	NP_012183.2
	PPCS	XP_001087174.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004311	XP_313585.4
Kluyveromyces lactis	KLLA0E08757g	XP_454344.1
Schizosaccharomyces pombe (fission yeast)	SPCC4B3.18	NP_588072.1
Xenopus tropicalis (tropical clawed frog)	ppcs	NP_001072632.1
Pan troglodytes (chimpanzee)	PPCS	XP_003949430.1
Drosophila melanogaster (fruit fly)	Ppcs	NP_732401.1
Oryza sativa (rice)	Os08g0176100	NP_001061116.1
Rattus norvegicus (Norway rat)	Ppcs	NP_001034099.2
Danio rerio (zebrafish)	ppcs	NP_001038705.1
Arabidopsis thaliana (thale cress)	COAB	NP_563904.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ppcs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppcs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05377	NM_169848.3 Latest version!	Drosophila melanogaster phosphopantothenoylcysteine synthetase, transcript variant A (Ppcs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa05377	NM_001316504.1 Latest version!	Drosophila melanogaster phosphopantothenoylcysteine synthetase, transcript variant D (Ppcs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa05378	NM_142527.3 Latest version!	Drosophila melanogaster phosphopantothenoylcysteine synthetase, transcript variant C (Ppcs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppcs ( NM_169848.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732401.1 Drosophila melanogaster phosphopantothenoylcysteine synthetase, transcript variant A (Ppcs), mRNA.

Price & Availability↑

CloneID	OFa05377
Clone ID Related Accession (Same CDS sequence)	NM_169848.3 , NM_001316504.1
Accession Version	NM_169848.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphopantothenoylcysteine synthetase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169848.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCACT GGGAGGACTT CTACAACACG CACCTGCCGC CCGCGGACTT CGAGGACAAT 
CGCTCCCTGC TCAAGGAGTT CTGCGAACGG CACAACAAGC TCCAGAATCG CATCGTCCTC 
GTCACGTCCG GTGGCACCAC AGTTCCCCTG GAGCACAATA CGGTGCGGTT TGTGGACAAT 
TTTAGTGCTG GAACGCGTGG CTCCGCCTCG GCGGAATACT TCCTCGACCA CGACTATGCC 
GTGATTTTCA TGCATCGCCA CAAGTCGCTG GAGCCCTTCA CGCGCCACTT CACCGGCCAG 
CAGTTCTTCG ACATGCTGGA CATTGCGGAC AACAGCCAGA GTTCGACGAT AGCCATCAAA 
CCGGATTCGG TGGACGTGTT TGCTCCGGTG CTGGCCAAAT ACAAGATAGC CCGCGAAACG 
CAGATGATCC TGTACGTGAA CTTCACCAGC GTGGTGGACT ACATGTGGCT GCTCCGCGCC 
GCCTGCGAGT GCCTGGCCGC CTTCGAGGAG CGAGCCGTGC TCTACCTAGC CGCAGCCGTA 
TCCGATTTCT ACATACCCGA GGACATGATG CCCACCCACA AAATGCAATC AGGAGATGGA 
GCGCCGACGA TTTCGCTGCA GCTGGTGCCA AAAATGCTGG CCCCCCTCGC CAGTTTGTGG 
GTCCCGCACG CCTTTGTGGT GTCCTTTAAA CTGGAAACGG ACGAGAGCCT ATTGATTGTG 
AAGGCACGTG ACAGTCTCAA CAAATACAAG CACAAGCTGG TCATTGCCAA TGTGCTGCAG 
ACACGCAAGC ATCGCGTGGT GTTCGTCACG CCCACAGATT CCTACGAGCT GCATTTGACT 
CGCGAACAGA CGCTGCAAGG ACTCGAGATC GAGGAGCCCA TCGTGGCCGA TGTGGTGCAA 
AAGCACGGCG AGTTCATCAG TAACGCTCAA CAGCGCCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732401.1
CDS	651..1592
Translation	MTHWEDFYNTHLPPADFEDNRSLLKEFCERHNKLQNRIVLVTSGGTTVPLEHNTVRFVDNFSAGTRGSASAEYFLDHDYAVIFMHRHKSLEPFTRHFTGQQFFDMLDIADNSQSSTIAIKPDSVDVFAPVLAKYKIARETQMILYVNFTSVVDYMWLLRAACECLAAFEERAVLYLAAAVSDFYIPEDMMPTHKMQSGDGAPTISLQLVPKMLAPLASLWVPHAFVVSFKLETDESLLIVKARDSLNKYKHKLVIANVLQTRKHRVVFVTPTDSYELHLTREQTLQGLEIEEPIVADVVQKHGEFISNAQQRQ

Target ORF information:

RefSeq Version	NM_169848.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphopantothenoylcysteine synthetase, transcript variant A (Ppcs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169848.3

ORF Insert Sequence:

ATGACGCACT GGGAGGACTT CTACAACACG CACCTGCCGC CCGCGGACTT CGAGGACAAT 
CGCTCCCTGC TCAAGGAGTT CTGCGAACGG CACAACAAGC TCCAGAATCG CATCGTCCTC 
GTCACGTCCG GTGGCACCAC AGTTCCCCTG GAGCACAATA CGGTGCGGTT TGTGGACAAT 
TTTAGTGCTG GAACGCGTGG CTCCGCCTCG GCGGAATACT TCCTCGACCA CGACTATGCC 
GTGATTTTCA TGCATCGCCA CAAGTCGCTG GAGCCCTTCA CGCGCCACTT CACCGGCCAG 
CAGTTCTTCG ACATGCTGGA CATTGCGGAC AACAGCCAGA GTTCGACGAT AGCCATCAAA 
CCGGATTCGG TGGACGTGTT TGCTCCGGTG CTGGCCAAAT ACAAGATAGC CCGCGAAACG 
CAGATGATCC TGTACGTGAA CTTCACCAGC GTGGTGGACT ACATGTGGCT GCTCCGCGCC 
GCCTGCGAGT GCCTGGCCGC CTTCGAGGAG CGAGCCGTGC TCTACCTAGC CGCAGCCGTA 
TCCGATTTCT ACATACCCGA GGACATGATG CCCACCCACA AAATGCAATC AGGAGATGGA 
GCGCCGACGA TTTCGCTGCA GCTGGTGCCA AAAATGCTGG CCCCCCTCGC CAGTTTGTGG 
GTCCCGCACG CCTTTGTGGT GTCCTTTAAA CTGGAAACGG ACGAGAGCCT ATTGATTGTG 
AAGGCACGTG ACAGTCTCAA CAAATACAAG CACAAGCTGG TCATTGCCAA TGTGCTGCAG 
ACACGCAAGC ATCGCGTGGT GTTCGTCACG CCCACAGATT CCTACGAGCT GCATTTGACT 
CGCGAACAGA CGCTGCAAGG ACTCGAGATC GAGGAGCCCA TCGTGGCCGA TGTGGTGCAA 
AAGCACGGCG AGTTCATCAG TAACGCTCAA CAGCGCCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppcs ( NM_001316504.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303433.1 Drosophila melanogaster phosphopantothenoylcysteine synthetase, transcript variant D (Ppcs), mRNA.

Price & Availability↑

CloneID	OFa05377
Clone ID Related Accession (Same CDS sequence)	NM_169848.3 , NM_001316504.1
Accession Version	NM_001316504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphopantothenoylcysteine synthetase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCACT GGGAGGACTT CTACAACACG CACCTGCCGC CCGCGGACTT CGAGGACAAT 
CGCTCCCTGC TCAAGGAGTT CTGCGAACGG CACAACAAGC TCCAGAATCG CATCGTCCTC 
GTCACGTCCG GTGGCACCAC AGTTCCCCTG GAGCACAATA CGGTGCGGTT TGTGGACAAT 
TTTAGTGCTG GAACGCGTGG CTCCGCCTCG GCGGAATACT TCCTCGACCA CGACTATGCC 
GTGATTTTCA TGCATCGCCA CAAGTCGCTG GAGCCCTTCA CGCGCCACTT CACCGGCCAG 
CAGTTCTTCG ACATGCTGGA CATTGCGGAC AACAGCCAGA GTTCGACGAT AGCCATCAAA 
CCGGATTCGG TGGACGTGTT TGCTCCGGTG CTGGCCAAAT ACAAGATAGC CCGCGAAACG 
CAGATGATCC TGTACGTGAA CTTCACCAGC GTGGTGGACT ACATGTGGCT GCTCCGCGCC 
GCCTGCGAGT GCCTGGCCGC CTTCGAGGAG CGAGCCGTGC TCTACCTAGC CGCAGCCGTA 
TCCGATTTCT ACATACCCGA GGACATGATG CCCACCCACA AAATGCAATC AGGAGATGGA 
GCGCCGACGA TTTCGCTGCA GCTGGTGCCA AAAATGCTGG CCCCCCTCGC CAGTTTGTGG 
GTCCCGCACG CCTTTGTGGT GTCCTTTAAA CTGGAAACGG ACGAGAGCCT ATTGATTGTG 
AAGGCACGTG ACAGTCTCAA CAAATACAAG CACAAGCTGG TCATTGCCAA TGTGCTGCAG 
ACACGCAAGC ATCGCGTGGT GTTCGTCACG CCCACAGATT CCTACGAGCT GCATTTGACT 
CGCGAACAGA CGCTGCAAGG ACTCGAGATC GAGGAGCCCA TCGTGGCCGA TGTGGTGCAA 
AAGCACGGCG AGTTCATCAG TAACGCTCAA CAGCGCCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303433.1
CDS	651..1592
Translation	MTHWEDFYNTHLPPADFEDNRSLLKEFCERHNKLQNRIVLVTSGGTTVPLEHNTVRFVDNFSAGTRGSASAEYFLDHDYAVIFMHRHKSLEPFTRHFTGQQFFDMLDIADNSQSSTIAIKPDSVDVFAPVLAKYKIARETQMILYVNFTSVVDYMWLLRAACECLAAFEERAVLYLAAAVSDFYIPEDMMPTHKMQSGDGAPTISLQLVPKMLAPLASLWVPHAFVVSFKLETDESLLIVKARDSLNKYKHKLVIANVLQTRKHRVVFVTPTDSYELHLTREQTLQGLEIEEPIVADVVQKHGEFISNAQQRQ

Target ORF information:

RefSeq Version	NM_001316504.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphopantothenoylcysteine synthetase, transcript variant D (Ppcs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316504.1

ORF Insert Sequence:

ATGACGCACT GGGAGGACTT CTACAACACG CACCTGCCGC CCGCGGACTT CGAGGACAAT 
CGCTCCCTGC TCAAGGAGTT CTGCGAACGG CACAACAAGC TCCAGAATCG CATCGTCCTC 
GTCACGTCCG GTGGCACCAC AGTTCCCCTG GAGCACAATA CGGTGCGGTT TGTGGACAAT 
TTTAGTGCTG GAACGCGTGG CTCCGCCTCG GCGGAATACT TCCTCGACCA CGACTATGCC 
GTGATTTTCA TGCATCGCCA CAAGTCGCTG GAGCCCTTCA CGCGCCACTT CACCGGCCAG 
CAGTTCTTCG ACATGCTGGA CATTGCGGAC AACAGCCAGA GTTCGACGAT AGCCATCAAA 
CCGGATTCGG TGGACGTGTT TGCTCCGGTG CTGGCCAAAT ACAAGATAGC CCGCGAAACG 
CAGATGATCC TGTACGTGAA CTTCACCAGC GTGGTGGACT ACATGTGGCT GCTCCGCGCC 
GCCTGCGAGT GCCTGGCCGC CTTCGAGGAG CGAGCCGTGC TCTACCTAGC CGCAGCCGTA 
TCCGATTTCT ACATACCCGA GGACATGATG CCCACCCACA AAATGCAATC AGGAGATGGA 
GCGCCGACGA TTTCGCTGCA GCTGGTGCCA AAAATGCTGG CCCCCCTCGC CAGTTTGTGG 
GTCCCGCACG CCTTTGTGGT GTCCTTTAAA CTGGAAACGG ACGAGAGCCT ATTGATTGTG 
AAGGCACGTG ACAGTCTCAA CAAATACAAG CACAAGCTGG TCATTGCCAA TGTGCTGCAG 
ACACGCAAGC ATCGCGTGGT GTTCGTCACG CCCACAGATT CCTACGAGCT GCATTTGACT 
CGCGAACAGA CGCTGCAAGG ACTCGAGATC GAGGAGCCCA TCGTGGCCGA TGTGGTGCAA 
AAGCACGGCG AGTTCATCAG TAACGCTCAA CAGCGCCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppcs ( NM_142527.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650784.3 Drosophila melanogaster phosphopantothenoylcysteine synthetase, transcript variant C (Ppcs), mRNA.

Price & Availability↑

CloneID	OFa05378
Clone ID Related Accession (Same CDS sequence)	NM_142527.3
Accession Version	NM_142527.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphopantothenoylcysteine synthetase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142527.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCACT GGGAGGACTT CTACAACACG CACCTGCCGC CCGCGGACTT CGAGGACAAT 
CGCTCCCTGC TCAAGGAGTT CTGCGAACGG CACAACAAGC TCCAGAATCG CATCGTCCTC 
GTCACGTCCG GTGGCACCAC AGTTCCCCTG GAGCACAATA CGGTGCGGTT TGTGGACAAT 
TTTAGTGCTG GAACGCGTGG CTCCGCCTCG GCGGAATACT TCCTCGACCA CGACTATGCC 
GTGATTTTCA TGCATCGCCA CAAGTCGCTG GAGCCCTTCA CGCGCCACTT CACCGGCCAG 
CAGTTCTTCG ACATGCTGGA CATTGCGGAC AACAGCCAGA GTTCGACGAT AGCCATCAAA 
CCGGATTCGG TGGACGTGTT TGCTCCGGTG CTGGCCAAAT ACAAGATAGC CCGCGAAACG 
CAGATGATCC TGTACGTGAA CTTCACCAGC GTGGTGGACT ACATGTGGCT GCTCCGCGCC 
GCCTGCGAGT GCCTGGCCGC CTTCGAGGAG CGAGCCGTGC TCTACCTAGC CGCAGCCGTA 
TCCGATTTCT ACATACCCGA GGACATGATG CCCACCCACA AAATGCAATC AGGAGATGGA 
GCGCCGACGA TTTCGCTGCA GCTGGTGCCA AAAATGCTGG CCCCCCTCGC CAGTTTGTGG 
GTCCCGCACG CCTTTGTGGT GTCCTTTAAA CTGGAAACGG ACGAGAGCCT ATTGATTGTG 
AAGGCACGTG ACAGTCTCAA CAAATACAAG CACAAGCTGG TCATTGCCAA TGTGCTGCAG 
ACACGCAAGC ATCGCGTGGT GTTCGTCACG CCCACAGATT CCTACGAGCT GCATTTGACT 
CGCGAACAGA CGCTGCAAGG ACTCGAGATC GAGGAGCCCA TCGTGGCCGA TGTGGTGCAA 
AAGCACGGCG AGTTCATCAG TAACGCTCAA CAGCGCCAAT GACCGTTGTC GATGCGCCGG 
AGGCCACAGA CGCAGCATCA GCAGCACCAC CACCAACAAC TGGCCACCGG TGACGAGGAC 
GAGGATGGCG GCGATCTTGA TCGGGATCCA TATTGCGTGA CCAGCTCGCA TTATTGCCGT 
GTTCTGAATC CGGGCACGCC AGGCTTTGCC CGCAAATATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650784.3
CDS	651..1772
Translation	MTHWEDFYNTHLPPADFEDNRSLLKEFCERHNKLQNRIVLVTSGGTTVPLEHNTVRFVDNFSAGTRGSASAEYFLDHDYAVIFMHRHKSLEPFTRHFTGQQFFDMLDIADNSQSSTIAIKPDSVDVFAPVLAKYKIARETQMILYVNFTSVVDYMWLLRAACECLAAFEERAVLYLAAAVSDFYIPEDMMPTHKMQSGDGAPTISLQLVPKMLAPLASLWVPHAFVVSFKLETDESLLIVKARDSLNKYKHKLVIANVLQTRKHRVVFVTPTDSYELHLTREQTLQGLEIEEPIVADVVQKHGEFISNAQQRQ*PLSMRRRPQTQHQQHHHQQLATGDEDEDGGDLDRDPYCVTSSHYCRVLNPGTPGFARKY

Target ORF information:

RefSeq Version	NM_142527.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphopantothenoylcysteine synthetase, transcript variant C (Ppcs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142527.3

ORF Insert Sequence:

ATGACGCACT GGGAGGACTT CTACAACACG CACCTGCCGC CCGCGGACTT CGAGGACAAT 
CGCTCCCTGC TCAAGGAGTT CTGCGAACGG CACAACAAGC TCCAGAATCG CATCGTCCTC 
GTCACGTCCG GTGGCACCAC AGTTCCCCTG GAGCACAATA CGGTGCGGTT TGTGGACAAT 
TTTAGTGCTG GAACGCGTGG CTCCGCCTCG GCGGAATACT TCCTCGACCA CGACTATGCC 
GTGATTTTCA TGCATCGCCA CAAGTCGCTG GAGCCCTTCA CGCGCCACTT CACCGGCCAG 
CAGTTCTTCG ACATGCTGGA CATTGCGGAC AACAGCCAGA GTTCGACGAT AGCCATCAAA 
CCGGATTCGG TGGACGTGTT TGCTCCGGTG CTGGCCAAAT ACAAGATAGC CCGCGAAACG 
CAGATGATCC TGTACGTGAA CTTCACCAGC GTGGTGGACT ACATGTGGCT GCTCCGCGCC 
GCCTGCGAGT GCCTGGCCGC CTTCGAGGAG CGAGCCGTGC TCTACCTAGC CGCAGCCGTA 
TCCGATTTCT ACATACCCGA GGACATGATG CCCACCCACA AAATGCAATC AGGAGATGGA 
GCGCCGACGA TTTCGCTGCA GCTGGTGCCA AAAATGCTGG CCCCCCTCGC CAGTTTGTGG 
GTCCCGCACG CCTTTGTGGT GTCCTTTAAA CTGGAAACGG ACGAGAGCCT ATTGATTGTG 
AAGGCACGTG ACAGTCTCAA CAAATACAAG CACAAGCTGG TCATTGCCAA TGTGCTGCAG 
ACACGCAAGC ATCGCGTGGT GTTCGTCACG CCCACAGATT CCTACGAGCT GCATTTGACT 
CGCGAACAGA CGCTGCAAGG ACTCGAGATC GAGGAGCCCA TCGTGGCCGA TGTGGTGCAA 
AAGCACGGCG AGTTCATCAG TAACGCTCAA CAGCGCCAAT GACCGTTGTC GATGCGCCGG 
AGGCCACAGA CGCAGCATCA GCAGCACCAC CACCAACAAC TGGCCACCGG TGACGAGGAC 
GAGGATGGCG GCGATCTTGA TCGGGATCCA TATTGCGTGA CCAGCTCGCA TTATTGCCGT 
GTTCTGAATC CGGGCACGCC AGGCTTTGCC CGCAAATATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ppcs cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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