EndoA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	EndoA
Entrez Gene ID	42265
Full Name	Endophilin A
Synonyms	CG14296,D-endo,D-endoA,Dendo-A,Dmel\CG14296,Endo,endo,endoA
General protein information	Preferred Names Endophilin A Names CG14296 gene product from transcript CG14296-RC CG14296-PA CG14296-PB CG14296-PC EndoA-PA EndoA-PB EndoA-PC Endophilin-A endophilin endophilin A endophilinA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91D4-91D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_169838.2	NP_732383.1	endophilin A, isoform A
NM_138767.3	NP_620122.2	endophilin A, isoform B
NM_001275788.1	NP_001262717.1	endophilin A, isoform C

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SH3GL3	XP_002696641.2
Gallus gallus (chicken)	SH3GL3	NP_989859.1
Homo sapiens (human)	SH3GL3	NP_003018.3
Pan troglodytes (chimpanzee)	SH3GL3	XP_001161355.1
Canis lupus familiaris (dog)	SH3GL3	XP_005618456.1
Mus musculus (house mouse)	Sh3gl3	NP_059096.3
Danio rerio (zebrafish)	sh3gl3a	NP_001075160.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004766	XP_318050.4
Caenorhabditis elegans (roundworm)	unc-57	NP_001040680.1
Schizosaccharomyces pombe (fission yeast)	mug137	NP_588493.1
Xenopus tropicalis (tropical clawed frog)	sh3gl3	XP_002940145.1
	SH3GL3	XP_001111376.2
Rattus norvegicus (Norway rat)	Sh3gl3	NP_112517.1
Drosophila melanogaster (fruit fly)	endoA	NP_001262717.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EndophilinA-induced macroautophagy is activated when the kinase LRRK2 phosphorylates the EndophilinA-BAR domain and is blocked in animals where EndophilinA cannot be phosphorylated. Functionally, deregulation of the EndophilinA phosphorylation state accelerates activity-induced neurodegeneration.
Title: A LRRK2-Dependent EndophilinA Phosphoswitch Is Critical for Macroautophagy at Presynaptic Terminals.

analysis of endophilin-A N-BAR domain mutations performed in living flies
Title: A mutational analysis of the endophilin-A N-BAR domain performed in living flies.

In the absence of synaptojanin or endophilin, vesicles undergo full fusion and re-formation.
Title: A slowed classical pathway rather than kiss-and-run mediates endocytosis at synapses lacking synaptojanin and endophilin.

At fly photoreceptor synapses Endophilin is localized to synaptic vesicles at sites of endocytosis that are glial invaginations called capitate projections; when the photoreceptor synapses lack Endophilin they are impaired in neurotransmitter release
Title: Endophilin promotes a late step in endocytosis at glial invaginations in Drosophila photoreceptor terminals.

Endophilin is required presynaptically at the neuromuscular junction, and absence of Endophilin dramatically impairs endocytosis in vivo
Title: Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter release.

The following EndoA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EndoA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05352	NM_138767.3 Latest version!	Drosophila melanogaster endophilin A, transcript variant B (EndoA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa05352	NM_001275788.1 Latest version!	Drosophila melanogaster endophilin A, transcript variant C (EndoA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa05352	NM_169838.2 Latest version!	Drosophila melanogaster endophilin A, transcript variant A (EndoA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EndoA ( NM_169838.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732383.1 Drosophila melanogaster endophilin A, transcript variant A (EndoA), mRNA.

Price & Availability↑

CloneID	OFa05352
Clone ID Related Accession (Same CDS sequence)	NM_169838.2 , NM_138767.3 , NM_001275788.1
Accession Version	NM_169838.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	endophilin A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169838.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTTCG CCGGACTCAA AAAGCAGATC AACAAGGCCA ACCAGTATAT GACGGAGAAG 
ATGGGCGGTG CCGAGGGCAC CAAACTGGAC ATGGACTTCA TGGAGATGGA ACGCAAGACG 
GACGTCACCG TGGAGCTAGT GGAGGAGCTG CAGCTAAAGA CGAAGGAGTT CCTGCAGCCG 
AATCCAACGG CACGGGCCAA AATGGCAGCG GTCAAGGGCA TCTCGAAGCT GTCCGGACAG 
GCCAAGTCCA ATACGTATCC GCAACCGGAG GGCCTGCTCG CGGAATGCAT GCTGACTTAT 
GGGAAGAAGC TCGGCGAGGA CAACAGCGTG TTCGCGCAGG CGCTCGTCGA ATTCGGCGAA 
GCGCTGAAAC AGATGGCCGA CGTCAAGTAT TCGCTGGACG ACAACATCAA GCAGAACTTT 
TTGGAGCCAC TGCATCATAT GCAGACCAAA GACCTCAAGG AGGTAATGCA TCATCGCAAG 
AAGCTGCAGG GCCGGCGGCT AGACTTTGAC TGCAAGCGTC GCCGACAGGC CAAGGACGAT 
GAGATTCGTG GTGCCGAGGA CAAGTTCGGT GAATCGCTCC AGCTGGCCCA GGTGGGCATG 
TTCAATTTGC TCGAGAACGA TACGGAGCAT GTCTCCCAGC TGGTCACCTT TGCCGAGGCA 
CTATACGATT TTCATTCGCA ATGCGCGGAT GTCCTTCGAG GCCTGCAGGA GACACTGCAG 
GAGAAGCGCT CCGAGGCGGA GAGCCGGCCA CGCAACGAGT TCGTGCCCAA GACGCTGCTC 
GATCTGAACT TGGACGGCGG TGGCGGCGGC CTCAACGAAG ATGGCACGCC GTCTCACATT 
AGTTCGAGCG CCTCGCCGTT GCCCTCGCCG ATGCGTTCGC CCGCCAAGTC GATGGCCGTA 
ACGCCGCAGC GCCAGCAGCA GCCCTGCTGC CAGGCCCTCT ACGACTTCGA GCCGGAGAAT 
CCCGGCGAAC TGGCCTTCAA GGAGAACGAC ATTATCACCC TGTTGAATCG CGTCGACGAC 
AATTGGTTCG AGGGCGCGGT GAATGGCCGC ACCGGTTACT TCCCGCAGTC GTATGTTCAG 
GTGCAGGTGC CCCTGCCCAA TGGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732383.1
CDS	480..1589
Translation	MAFAGLKKQINKANQYMTEKMGGAEGTKLDMDFMEMERKTDVTVELVEELQLKTKEFLQPNPTARAKMAAVKGISKLSGQAKSNTYPQPEGLLAECMLTYGKKLGEDNSVFAQALVEFGEALKQMADVKYSLDDNIKQNFLEPLHHMQTKDLKEVMHHRKKLQGRRLDFDCKRRRQAKDDEIRGAEDKFGESLQLAQVGMFNLLENDTEHVSQLVTFAEALYDFHSQCADVLRGLQETLQEKRSEAESRPRNEFVPKTLLDLNLDGGGGGLNEDGTPSHISSSASPLPSPMRSPAKSMAVTPQRQQQPCCQALYDFEPENPGELAFKENDIITLLNRVDDNWFEGAVNGRTGYFPQSYVQVQVPLPNGN

Target ORF information:

RefSeq Version	NM_169838.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster endophilin A, transcript variant A (EndoA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169838.2

ORF Insert Sequence:

ATGGCTTTCG CCGGACTCAA AAAGCAGATC AACAAGGCCA ACCAGTATAT GACGGAGAAG 
ATGGGCGGTG CCGAGGGCAC CAAACTGGAC ATGGACTTCA TGGAGATGGA ACGCAAGACG 
GACGTCACCG TGGAGCTAGT GGAGGAGCTG CAGCTAAAGA CGAAGGAGTT CCTGCAGCCG 
AATCCAACGG CACGGGCCAA AATGGCAGCG GTCAAGGGCA TCTCGAAGCT GTCCGGACAG 
GCCAAGTCCA ATACGTATCC GCAACCGGAG GGCCTGCTCG CGGAATGCAT GCTGACTTAT 
GGGAAGAAGC TCGGCGAGGA CAACAGCGTG TTCGCGCAGG CGCTCGTCGA ATTCGGCGAA 
GCGCTGAAAC AGATGGCCGA CGTCAAGTAT TCGCTGGACG ACAACATCAA GCAGAACTTT 
TTGGAGCCAC TGCATCATAT GCAGACCAAA GACCTCAAGG AGGTAATGCA TCATCGCAAG 
AAGCTGCAGG GCCGGCGGCT AGACTTTGAC TGCAAGCGTC GCCGACAGGC CAAGGACGAT 
GAGATTCGTG GTGCCGAGGA CAAGTTCGGT GAATCGCTCC AGCTGGCCCA GGTGGGCATG 
TTCAATTTGC TCGAGAACGA TACGGAGCAT GTCTCCCAGC TGGTCACCTT TGCCGAGGCA 
CTATACGATT TTCATTCGCA ATGCGCGGAT GTCCTTCGAG GCCTGCAGGA GACACTGCAG 
GAGAAGCGCT CCGAGGCGGA GAGCCGGCCA CGCAACGAGT TCGTGCCCAA GACGCTGCTC 
GATCTGAACT TGGACGGCGG TGGCGGCGGC CTCAACGAAG ATGGCACGCC GTCTCACATT 
AGTTCGAGCG CCTCGCCGTT GCCCTCGCCG ATGCGTTCGC CCGCCAAGTC GATGGCCGTA 
ACGCCGCAGC GCCAGCAGCA GCCCTGCTGC CAGGCCCTCT ACGACTTCGA GCCGGAGAAT 
CCCGGCGAAC TGGCCTTCAA GGAGAACGAC ATTATCACCC TGTTGAATCG CGTCGACGAC 
AATTGGTTCG AGGGCGCGGT GAATGGCCGC ACCGGTTACT TCCCGCAGTC GTATGTTCAG 
GTGCAGGTGC CCCTGCCCAA TGGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EndoA ( NM_138767.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_620122.2 Drosophila melanogaster endophilin A, transcript variant B (EndoA), mRNA.

Price & Availability↑

CloneID	OFa05352
Clone ID Related Accession (Same CDS sequence)	NM_169838.2 , NM_138767.3 , NM_001275788.1
Accession Version	NM_138767.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	endophilin A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_138767.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTTCG CCGGACTCAA AAAGCAGATC AACAAGGCCA ACCAGTATAT GACGGAGAAG 
ATGGGCGGTG CCGAGGGCAC CAAACTGGAC ATGGACTTCA TGGAGATGGA ACGCAAGACG 
GACGTCACCG TGGAGCTAGT GGAGGAGCTG CAGCTAAAGA CGAAGGAGTT CCTGCAGCCG 
AATCCAACGG CACGGGCCAA AATGGCAGCG GTCAAGGGCA TCTCGAAGCT GTCCGGACAG 
GCCAAGTCCA ATACGTATCC GCAACCGGAG GGCCTGCTCG CGGAATGCAT GCTGACTTAT 
GGGAAGAAGC TCGGCGAGGA CAACAGCGTG TTCGCGCAGG CGCTCGTCGA ATTCGGCGAA 
GCGCTGAAAC AGATGGCCGA CGTCAAGTAT TCGCTGGACG ACAACATCAA GCAGAACTTT 
TTGGAGCCAC TGCATCATAT GCAGACCAAA GACCTCAAGG AGGTAATGCA TCATCGCAAG 
AAGCTGCAGG GCCGGCGGCT AGACTTTGAC TGCAAGCGTC GCCGACAGGC CAAGGACGAT 
GAGATTCGTG GTGCCGAGGA CAAGTTCGGT GAATCGCTCC AGCTGGCCCA GGTGGGCATG 
TTCAATTTGC TCGAGAACGA TACGGAGCAT GTCTCCCAGC TGGTCACCTT TGCCGAGGCA 
CTATACGATT TTCATTCGCA ATGCGCGGAT GTCCTTCGAG GCCTGCAGGA GACACTGCAG 
GAGAAGCGCT CCGAGGCGGA GAGCCGGCCA CGCAACGAGT TCGTGCCCAA GACGCTGCTC 
GATCTGAACT TGGACGGCGG TGGCGGCGGC CTCAACGAAG ATGGCACGCC GTCTCACATT 
AGTTCGAGCG CCTCGCCGTT GCCCTCGCCG ATGCGTTCGC CCGCCAAGTC GATGGCCGTA 
ACGCCGCAGC GCCAGCAGCA GCCCTGCTGC CAGGCCCTCT ACGACTTCGA GCCGGAGAAT 
CCCGGCGAAC TGGCCTTCAA GGAGAACGAC ATTATCACCC TGTTGAATCG CGTCGACGAC 
AATTGGTTCG AGGGCGCGGT GAATGGCCGC ACCGGTTACT TCCCGCAGTC GTATGTTCAG 
GTGCAGGTGC CCCTGCCCAA TGGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620122.2
CDS	659..1768
Translation	MAFAGLKKQINKANQYMTEKMGGAEGTKLDMDFMEMERKTDVTVELVEELQLKTKEFLQPNPTARAKMAAVKGISKLSGQAKSNTYPQPEGLLAECMLTYGKKLGEDNSVFAQALVEFGEALKQMADVKYSLDDNIKQNFLEPLHHMQTKDLKEVMHHRKKLQGRRLDFDCKRRRQAKDDEIRGAEDKFGESLQLAQVGMFNLLENDTEHVSQLVTFAEALYDFHSQCADVLRGLQETLQEKRSEAESRPRNEFVPKTLLDLNLDGGGGGLNEDGTPSHISSSASPLPSPMRSPAKSMAVTPQRQQQPCCQALYDFEPENPGELAFKENDIITLLNRVDDNWFEGAVNGRTGYFPQSYVQVQVPLPNGN

Target ORF information:

RefSeq Version	NM_138767.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster endophilin A, transcript variant B (EndoA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138767.3

ORF Insert Sequence:

ATGGCTTTCG CCGGACTCAA AAAGCAGATC AACAAGGCCA ACCAGTATAT GACGGAGAAG 
ATGGGCGGTG CCGAGGGCAC CAAACTGGAC ATGGACTTCA TGGAGATGGA ACGCAAGACG 
GACGTCACCG TGGAGCTAGT GGAGGAGCTG CAGCTAAAGA CGAAGGAGTT CCTGCAGCCG 
AATCCAACGG CACGGGCCAA AATGGCAGCG GTCAAGGGCA TCTCGAAGCT GTCCGGACAG 
GCCAAGTCCA ATACGTATCC GCAACCGGAG GGCCTGCTCG CGGAATGCAT GCTGACTTAT 
GGGAAGAAGC TCGGCGAGGA CAACAGCGTG TTCGCGCAGG CGCTCGTCGA ATTCGGCGAA 
GCGCTGAAAC AGATGGCCGA CGTCAAGTAT TCGCTGGACG ACAACATCAA GCAGAACTTT 
TTGGAGCCAC TGCATCATAT GCAGACCAAA GACCTCAAGG AGGTAATGCA TCATCGCAAG 
AAGCTGCAGG GCCGGCGGCT AGACTTTGAC TGCAAGCGTC GCCGACAGGC CAAGGACGAT 
GAGATTCGTG GTGCCGAGGA CAAGTTCGGT GAATCGCTCC AGCTGGCCCA GGTGGGCATG 
TTCAATTTGC TCGAGAACGA TACGGAGCAT GTCTCCCAGC TGGTCACCTT TGCCGAGGCA 
CTATACGATT TTCATTCGCA ATGCGCGGAT GTCCTTCGAG GCCTGCAGGA GACACTGCAG 
GAGAAGCGCT CCGAGGCGGA GAGCCGGCCA CGCAACGAGT TCGTGCCCAA GACGCTGCTC 
GATCTGAACT TGGACGGCGG TGGCGGCGGC CTCAACGAAG ATGGCACGCC GTCTCACATT 
AGTTCGAGCG CCTCGCCGTT GCCCTCGCCG ATGCGTTCGC CCGCCAAGTC GATGGCCGTA 
ACGCCGCAGC GCCAGCAGCA GCCCTGCTGC CAGGCCCTCT ACGACTTCGA GCCGGAGAAT 
CCCGGCGAAC TGGCCTTCAA GGAGAACGAC ATTATCACCC TGTTGAATCG CGTCGACGAC 
AATTGGTTCG AGGGCGCGGT GAATGGCCGC ACCGGTTACT TCCCGCAGTC GTATGTTCAG 
GTGCAGGTGC CCCTGCCCAA TGGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EndoA ( NM_001275788.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262717.1 Drosophila melanogaster endophilin A, transcript variant C (EndoA), mRNA.

Price & Availability↑

CloneID	OFa05352
Clone ID Related Accession (Same CDS sequence)	NM_169838.2 , NM_138767.3 , NM_001275788.1
Accession Version	NM_001275788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	endophilin A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTTCG CCGGACTCAA AAAGCAGATC AACAAGGCCA ACCAGTATAT GACGGAGAAG 
ATGGGCGGTG CCGAGGGCAC CAAACTGGAC ATGGACTTCA TGGAGATGGA ACGCAAGACG 
GACGTCACCG TGGAGCTAGT GGAGGAGCTG CAGCTAAAGA CGAAGGAGTT CCTGCAGCCG 
AATCCAACGG CACGGGCCAA AATGGCAGCG GTCAAGGGCA TCTCGAAGCT GTCCGGACAG 
GCCAAGTCCA ATACGTATCC GCAACCGGAG GGCCTGCTCG CGGAATGCAT GCTGACTTAT 
GGGAAGAAGC TCGGCGAGGA CAACAGCGTG TTCGCGCAGG CGCTCGTCGA ATTCGGCGAA 
GCGCTGAAAC AGATGGCCGA CGTCAAGTAT TCGCTGGACG ACAACATCAA GCAGAACTTT 
TTGGAGCCAC TGCATCATAT GCAGACCAAA GACCTCAAGG AGGTAATGCA TCATCGCAAG 
AAGCTGCAGG GCCGGCGGCT AGACTTTGAC TGCAAGCGTC GCCGACAGGC CAAGGACGAT 
GAGATTCGTG GTGCCGAGGA CAAGTTCGGT GAATCGCTCC AGCTGGCCCA GGTGGGCATG 
TTCAATTTGC TCGAGAACGA TACGGAGCAT GTCTCCCAGC TGGTCACCTT TGCCGAGGCA 
CTATACGATT TTCATTCGCA ATGCGCGGAT GTCCTTCGAG GCCTGCAGGA GACACTGCAG 
GAGAAGCGCT CCGAGGCGGA GAGCCGGCCA CGCAACGAGT TCGTGCCCAA GACGCTGCTC 
GATCTGAACT TGGACGGCGG TGGCGGCGGC CTCAACGAAG ATGGCACGCC GTCTCACATT 
AGTTCGAGCG CCTCGCCGTT GCCCTCGCCG ATGCGTTCGC CCGCCAAGTC GATGGCCGTA 
ACGCCGCAGC GCCAGCAGCA GCCCTGCTGC CAGGCCCTCT ACGACTTCGA GCCGGAGAAT 
CCCGGCGAAC TGGCCTTCAA GGAGAACGAC ATTATCACCC TGTTGAATCG CGTCGACGAC 
AATTGGTTCG AGGGCGCGGT GAATGGCCGC ACCGGTTACT TCCCGCAGTC GTATGTTCAG 
GTGCAGGTGC CCCTGCCCAA TGGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262717.1
CDS	480..1589
Translation	MAFAGLKKQINKANQYMTEKMGGAEGTKLDMDFMEMERKTDVTVELVEELQLKTKEFLQPNPTARAKMAAVKGISKLSGQAKSNTYPQPEGLLAECMLTYGKKLGEDNSVFAQALVEFGEALKQMADVKYSLDDNIKQNFLEPLHHMQTKDLKEVMHHRKKLQGRRLDFDCKRRRQAKDDEIRGAEDKFGESLQLAQVGMFNLLENDTEHVSQLVTFAEALYDFHSQCADVLRGLQETLQEKRSEAESRPRNEFVPKTLLDLNLDGGGGGLNEDGTPSHISSSASPLPSPMRSPAKSMAVTPQRQQQPCCQALYDFEPENPGELAFKENDIITLLNRVDDNWFEGAVNGRTGYFPQSYVQVQVPLPNGN

Target ORF information:

RefSeq Version	NM_001275788.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster endophilin A, transcript variant C (EndoA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275788.1

ORF Insert Sequence:

ATGGCTTTCG CCGGACTCAA AAAGCAGATC AACAAGGCCA ACCAGTATAT GACGGAGAAG 
ATGGGCGGTG CCGAGGGCAC CAAACTGGAC ATGGACTTCA TGGAGATGGA ACGCAAGACG 
GACGTCACCG TGGAGCTAGT GGAGGAGCTG CAGCTAAAGA CGAAGGAGTT CCTGCAGCCG 
AATCCAACGG CACGGGCCAA AATGGCAGCG GTCAAGGGCA TCTCGAAGCT GTCCGGACAG 
GCCAAGTCCA ATACGTATCC GCAACCGGAG GGCCTGCTCG CGGAATGCAT GCTGACTTAT 
GGGAAGAAGC TCGGCGAGGA CAACAGCGTG TTCGCGCAGG CGCTCGTCGA ATTCGGCGAA 
GCGCTGAAAC AGATGGCCGA CGTCAAGTAT TCGCTGGACG ACAACATCAA GCAGAACTTT 
TTGGAGCCAC TGCATCATAT GCAGACCAAA GACCTCAAGG AGGTAATGCA TCATCGCAAG 
AAGCTGCAGG GCCGGCGGCT AGACTTTGAC TGCAAGCGTC GCCGACAGGC CAAGGACGAT 
GAGATTCGTG GTGCCGAGGA CAAGTTCGGT GAATCGCTCC AGCTGGCCCA GGTGGGCATG 
TTCAATTTGC TCGAGAACGA TACGGAGCAT GTCTCCCAGC TGGTCACCTT TGCCGAGGCA 
CTATACGATT TTCATTCGCA ATGCGCGGAT GTCCTTCGAG GCCTGCAGGA GACACTGCAG 
GAGAAGCGCT CCGAGGCGGA GAGCCGGCCA CGCAACGAGT TCGTGCCCAA GACGCTGCTC 
GATCTGAACT TGGACGGCGG TGGCGGCGGC CTCAACGAAG ATGGCACGCC GTCTCACATT 
AGTTCGAGCG CCTCGCCGTT GCCCTCGCCG ATGCGTTCGC CCGCCAAGTC GATGGCCGTA 
ACGCCGCAGC GCCAGCAGCA GCCCTGCTGC CAGGCCCTCT ACGACTTCGA GCCGGAGAAT 
CCCGGCGAAC TGGCCTTCAA GGAGAACGAC ATTATCACCC TGTTGAATCG CGTCGACGAC 
AATTGGTTCG AGGGCGCGGT GAATGGCCGC ACCGGTTACT TCCCGCAGTC GTATGTTCAG 
GTGCAGGTGC CCCTGCCCAA TGGCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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EndoA cDNA ORF clone, Drosophila melanogaster(fruit fly)

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