Mekk1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mekk1
Entrez Gene ID	42253
Full Name	CG7717 gene product from transcript CG7717-RB
Synonyms	CG7717,D-MEKK1,D-Mekk1,DmMEKK1,Dmel\CG7717,MEKK,MEKK1,MEKK4,Mekk1/4,dMEKK,dMEKK1,dMEKK4,dMekk1,mekk1,mekk1a,mekk1b
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91C5-91C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_169833.2	NP_732373.1	Mekk1, isoform B
NM_142493.3	NP_650750.4	Mekk1, isoform D

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Map3k4	NP_036078.2
Gallus gallus (chicken)	MAP3K4	XP_004935618.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002371	XP_312585.5
	MAP3K4	XP_001098969.2
Canis lupus familiaris (dog)	MAP3K4	XP_005615756.1
Danio rerio (zebrafish)	map3k4	XP_005157074.1
Xenopus tropicalis (tropical clawed frog)	map3k4	XP_004914705.1
Pan troglodytes (chimpanzee)	MAP3K4	XP_003311625.2
Bos taurus (cattle)	MAP3K4	XP_002690423.2
Rattus norvegicus (Norway rat)	Map3k4	NP_001100926.1
Drosophila melanogaster (fruit fly)	Mekk1	NP_650750.4
Homo sapiens (human)	MAP3K4	NP_005913.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The CDK5 phosphorylates MEKK1, and together, they activate the JNK pathway for apoptosis.
Title: CDK5 and MEKK1 mediate pro-apoptotic signalling following endoplasmic reticulum stress in an autosomal dominant retinitis pigmentosa model.

D-MEKK1, the fly orthologue of mammalian MEKK4/MTK1, and Hemipterous/D-MKK7 mediates the activation of D-JNK by cadmium and arsenite.
Title: D-MEKK1, the Drosophila orthologue of mammalian MEKK4/MTK1, and Hemipterous/D-MKK7 mediate the activation of D-JNK by cadmium and arsenite in Schneider cells.

The following Mekk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mekk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05341	NM_169833.2 Latest version!	Drosophila melanogaster Mekk1, transcript variant B (Mekk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa05342	NM_142493.3 Latest version!	Drosophila melanogaster Mekk1, transcript variant D (Mekk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mekk1 ( NM_169833.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732373.1 Drosophila melanogaster Mekk1, transcript variant B (Mekk1), mRNA.

Price & Availability↑

CloneID	OFa05341
Clone ID Related Accession (Same CDS sequence)	NM_169833.2
Accession Version	NM_169833.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Mekk1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169833.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAAACA GGCGAAGAGT GCGAACAATT GATTATCTGG CGCTGCAGCA GAGTTTGCGT 
TTGCAAAAGA CACCGGCAGC AACAACAAAT GCAGAGGAGC AGGTGGCAAG GGAGGAGGAG 
AATGGCAATG GGCATCATAG TACCGTTACA GCTGAAACGC CACCCACACC ACCCATCCCG 
CCCATTCCGC CCATACGATT ACGACGCGAG CAGAGCGTCG AGGAGGATGT TGCAAGAGTT 
GAGTACCGTG TGAAGCAGAC GCCTTCGCGT CCAGTGCAGA TGACTCGAAA TCGGATTGGT 
GCTTTGGAGG AGGACATGCC TCCCGAAGAT GAGCTGGCGG CGCACTATGA GGCCTTCGGC 
ACTACTCCGC CACGTACCAG GCTCAAAATC AAGAACCGCG ACTGGGAACG CAAACAGAAG 
GTGGTCAATG TGACAGCTTC CGCAGACTTA CCGGCCAGTG TGGGTGGCAC CCCCAAAAAG 
ACCAGGACGG CCAGATCCCG CGTCCTGCGG CGCAACACTA TGGACTGCGC CCTGCTCAAC 
GAGATGTTTG TCAACGATGA GAGCAAGCGG ACGGACAAGC GGCTGCAGTC ACTCCTTCGC 
GACTCGGAAC GTGAGATGAA GAACTCGTTG GCCACAACGG CGGTGGCTGC TCCACGAGGT 
AACAGCTTCC ACGAGACAGC TCATCCGCTG GAGTCTCTGG ACCAGATCAT GCCGCTCAAT 
TCGGAGGCAA TGGCGCCGAT TCCACTGCGA ATTGCTTCCA AGGTGGTGGA GAGCTGCAAT 
CGCTATCGCT GCGTGTCCTC ACGTCCCATT GGGTATCGCT CCTCGGCGCC GCCGTTGGCT 
TTCGCTGCCC AACTTCCCGC TGGAATGCTA AGGAGTGATG GAAGAGCAAC ACCTGGGTTG 
GGGAAAAGAA AGGACTTCCA CGAAACGTTT GCCAATCTAA TTAAGCTCGG TAGCGTAGAT 
CGACAGGACG CCAAACTATC ACAGGAGGAG CACACCTGGC AGACTGAGCT GAAGGATCTC 
ATCTGGCTAG AGCTACAGGC CTGGCAAGCA GACCGAACTG TGGAGCAGCA GGACAAGTAC 
CTTTTCGAAG CGCGTCAGGG AGTCTCCGAT CTGCTCACCC ACATTATAAA CTACAAGTTC 
CAGCCGCGTT ATCGCCGCGA GCCGAGTCTG ATAAGCTTGG ATAGTGGCAC TCATTCCGAT 
AGCAATTCTA ATGCCAGCTC TCCACTGCCC AGCAAGATGT GCCAAGGATG CATGTCGCTG 
TACTGCAAGG ATTGCATGGA TCATCAGGAG TTGGCGCTGC GAGAAGTGGA GGGTATGCTG 
ACGCGCCTGG AAGCTGCAGA GGCACTCTAC CCTTCCTCAC AGGCCATGGG CGCCCTGCAT 
CCCATTTACA AATCACAGAG CTTTGTAGGG CGCATCAAGT CCATGTGCTT ATGGTATAAC 
ATTACCAAGC AGAACAAGTT GAAGCTCAGT ATTTTAGGAA AAATTCTGGC AAGACTGCAG 
GATGAGAAGT TCTCTTGGCC CGTGTGCACT TCCTCCTACA TCGCCACCGA CAGCGGTAGT 
TCATCAGCAT CGGGTGTAGA GAACGATGAT TCGGCTGTTA ACTCAATGGA TTCATCAAAG 
CCGCCCAGTA TGGCCGGATC AGCCTCGCGA AAGGGTGTGA CACCGTGCCA CAAAGTGCAA 
TTCATGTTAA ACGACGCCAC CCACGTGCCA GGCGAGACAT CAAGTAGCAA TGAATCCACC 
TCCACGGAGG TAAGCCAGTG GTCCAGCGAG TGCTCTCACA GCCATATGCG TAAGGGATCC 
ATGCACGACA TCAACATTTT CAGTGTCGAG CCATTGGGTA CGTGCAGCAG CAATGGGACA 
GGAGAGCAAT CCACTGGGCT GTATCGCAAG TTCATTGAAA ATGTGCTCAA AAGTCGAGGA 
CTGGCCAAGT CGCTGGCTTT TCTACACAAG CTGCACAACG TGGCATTGTA CAAGGCGCAC 
ATAGCTCTGG AAAAACCCGG AGCTGAAGAC TTCGATTACG AGTCGGATGC AGAGTCCATT 
GAAGAGGACG TACCAAGGCT AGATCCTGAA ATCAGCCGCG AACAGGTTGT GGAGCTGCGC 
ACCTACGGCT ACTGGAGCGA GGAGGCACAA TCCATAAACC TGCCGTCGTA CATTCCCACC 
TTTGTATTTC TGAGCGGAAT TCCGTTGCAG TTCATGCATG AGTTCTTGCG CATGCGTCTC 
GAAACCAGAC CCGTTCGACC CAATCCACTG AGCCTGGAAC AGTTGATGAA GGAGCTTAGG 
GAGGGCTTGA CCCTAGCCTT GACCCATCGG GAGCGATACC AGCGGCACAT AACCACGGCG 
CTGGTGGAGA ATGAGGCGGA ACTGGGCAGT TATATTAGCA TTTTAAACCA CTACGATGCT 
ACGGTGCGAA AGACTTTCGA GCTGTATTTG GAGTACATCG ATCAACTAGT GCTGGTTGCC 
GTTCCGGAAG GCAACCAGAA GTCCGTCCTT GAGAAGGAAT GGATGTTTAC CAAGCTTATC 
AGTCCCATGA TAAAGGGCAT GCACACGTTA GCATCTCAAA AATTCTGTGG CATAATCAGC 
AAACTTTTGC GGAGCATTTC CGAACGCCTG GTGAAGCGCA CCGTTGAGCT AGATCAACAG 
ATCGATGGCA CCGCCGATAC CGACGACAAC GAGGAGGTTA AATGGCAGCT GCTAACCATT 
TGCAGAGAGA CACAATCGCT GCTCACAGTC GAACGGGAGC GCTCCATTAA GGTGCTCTTC 
TTTGCCAAAA CCTTTTGTCG CGACGTCGAG ACGACGGATT TTCATCGCGA GCATTATGAA 
CATGACGTGG CCAACCAGCA GCATGACTTC ATCTGTTCGG ACGTAAAGGC GGCGTTCAAG 
CTCTTGCAGC AGGACGTTCT GCAAGTGCGC AACAAGTTGA CGGCAATAAT TGAAGGAGTG 
CAAAAACGGT GCTGTTTGAG CAACATGCGA GATTTGGATG AGCAAGACAA ACAGGCTGTG 
CTGTCGCGCA CTCGAGAGAT TTTGCATCAG GGATACAAAT TTGGATTTGA ATATCACAAG 
GACGTCATTC GGTTGTTCGA GCAGAAGATT ATGGACCAAA AGGACAGCGG TGCTCACACA 
GTAGATCTTG CCTTAGGAAT TATCGCTTAC GCTAAGATGT GGATGCATTT TGTAATGGAG 
CGTTGCGAAC GTGGACGAGG AATGCGTCCC CGTTGGGCCT CCCAGGGTCT GGAGTTTCTG 
ATTCTTGCCT GTGATCCACA AATCACCCAG CACTTGGACG ATGACGAGTT TGAGGCGCTA 
AAGCAGCAAA TGGATCGCTG TATTTCGCAC GTGATTGGCA TCACCTCGGA ACCCGAAAAG 
GTCGCCAAGA AAAAGGCTTC GCCGCGCACT CGCAAGACTT CATCGCCGGC CACCTCGCGT 
TCCCGGACAC CAACACGGAC TCCAATGTCC GCTGGCATGG TCCTTAATCC GAATACGCCG 
CCACTACAGT CGCCACCGTA CAACAAGTTA CTTCATCCGC AGTTCAGTTT AAAGGAGGAT 
GTGTCAGGAA CTTCGTACAG TCCCGTTGAC AGTTCAGACT ATGTTGACAC TCCGTGCCAA 
AGGAGTGCCA ACGGCGAGCT GCGTCTGCTG GTGCCCCAGA CGCCGCCGAC TCCAGCATCT 
CCCGGAAAGA GCAGCCTCGA AAGTACACCG CTGGCCTTGC GGCAGGAACG TGTTAGAGAT 
GCCGTCAACC GTTTGGATAT GGATCTAGAG GACGGGCTAC GCGAACGAAG GTTGATTGGG 
CAGGTCAAGT CTCTTAATTC CAGCGACAAG GTGCATATAA GGGCGCGTAG CGTTCATTTC 
CGCTGGCATC GTGGCATTAA GATCGGCCAG GGACGATTCG GCAAGGTATA CACGGCGGTG 
AACAATAACA CGGGAGAGCT GATGGCCATG AAAGAGATCG CAATCCAGCC GGGAGAGACG 
CGAGCACTCA AGAATGTGGC CGAAGAGCTA AAGATCTTGG AAGGAATAAA GCACAAAAAC 
CTGGTGCGTT ACTATGGCAT CGAGGTGCAC CGCGAAGAGC TGCTTATTTT CATGGAGCTG 
TGCTCTGAGG GCACCCTCGA GTCACTGGTG GAGCTGACTG GTAATCTGCC GGAGGCGCTT 
ACGCGACGTT TCACCGCCCA GCTGTTGTCG GGCGTGTCTG AGCTGCACAA GCATGGCATT 
GTGCATCGCG ACATCAAGAC TGCTAACATA TTCCTCGTGG ACGGCAGCAA CAGCCTAAAA 
CTGGGCGATT TCGGATCAGC AGTGAAGATC CAAGCGCACA CCACTGTGCC CGGTGAGCTG 
CAGGGCTATG TAGGCACGCA GGCCTATATG GCGCCGGAGG TTTTCACAAA GACCAACAGC 
GATGGCCATG GCAGGGCGGC CGATATCTGG TCGGTGGGCT GTGTGGTTGT CGAGATGGCC 
TCGGGCAAGC GTCCTTGGGC CCAGTTTGAT TCCAACTTCC AGATCATGTT CAAAGTGGGC 
ATGGGCGAGA AGCCGCAGGC ACCGGAGAGC CTATCCCAGG AGGGTCACGA CTTCATCGAT 
CATTGTCTGC AGCACGATCC CAAGAGGCGT TTAACGGCAG TGGAACTGTT GGAGCACAAT 
TTCTGCAAGT ACGGTCGAGA CGAGTGCAGC AGTGAGCAGT TGCAGATGCA GGTGCGAGGC 
TCTTTCCGGC GAAATGTGGC GACCAGCAGC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732373.1
CDS	359..5074
Translation	MSNRRRVRTIDYLALQQSLRLQKTPAATTNAEEQVAREEENGNGHHSTVTAETPPTPPIPPIPPIRLRREQSVEEDVARVEYRVKQTPSRPVQMTRNRIGALEEDMPPEDELAAHYEAFGTTPPRTRLKIKNRDWERKQKVVNVTASADLPASVGGTPKKTRTARSRVLRRNTMDCALLNEMFVNDESKRTDKRLQSLLRDSEREMKNSLATTAVAAPRGNSFHETAHPLESLDQIMPLNSEAMAPIPLRIASKVVESCNRYRCVSSRPIGYRSSAPPLAFAAQLPAGMLRSDGRATPGLGKRKDFHETFANLIKLGSVDRQDAKLSQEEHTWQTELKDLIWLELQAWQADRTVEQQDKYLFEARQGVSDLLTHIINYKFQPRYRREPSLISLDSGTHSDSNSNASSPLPSKMCQGCMSLYCKDCMDHQELALREVEGMLTRLEAAEALYPSSQAMGALHPIYKSQSFVGRIKSMCLWYNITKQNKLKLSILGKILARLQDEKFSWPVCTSSYIATDSGSSSASGVENDDSAVNSMDSSKPPSMAGSASRKGVTPCHKVQFMLNDATHVPGETSSSNESTSTEVSQWSSECSHSHMRKGSMHDINIFSVEPLGTCSSNGTGEQSTGLYRKFIENVLKSRGLAKSLAFLHKLHNVALYKAHIALEKPGAEDFDYESDAESIEEDVPRLDPEISREQVVELRTYGYWSEEAQSINLPSYIPTFVFLSGIPLQFMHEFLRMRLETRPVRPNPLSLEQLMKELREGLTLALTHRERYQRHITTALVENEAELGSYISILNHYDATVRKTFELYLEYIDQLVLVAVPEGNQKSVLEKEWMFTKLISPMIKGMHTLASQKFCGIISKLLRSISERLVKRTVELDQQIDGTADTDDNEEVKWQLLTICRETQSLLTVERERSIKVLFFAKTFCRDVETTDFHREHYEHDVANQQHDFICSDVKAAFKLLQQDVLQVRNKLTAIIEGVQKRCCLSNMRDLDEQDKQAVLSRTREILHQGYKFGFEYHKDVIRLFEQKIMDQKDSGAHTVDLALGIIAYAKMWMHFVMERCERGRGMRPRWASQGLEFLILACDPQITQHLDDDEFEALKQQMDRCISHVIGITSEPEKVAKKKASPRTRKTSSPATSRSRTPTRTPMSAGMVLNPNTPPLQSPPYNKLLHPQFSLKEDVSGTSYSPVDSSDYVDTPCQRSANGELRLLVPQTPPTPASPGKSSLESTPLALRQERVRDAVNRLDMDLEDGLRERRLIGQVKSLNSSDKVHIRARSVHFRWHRGIKIGQGRFGKVYTAVNNNTGELMAMKEIAIQPGETRALKNVAEELKILEGIKHKNLVRYYGIEVHREELLIFMELCSEGTLESLVELTGNLPEALTRRFTAQLLSGVSELHKHGIVHRDIKTANIFLVDGSNSLKLGDFGSAVKIQAHTTVPGELQGYVGTQAYMAPEVFTKTNSDGHGRAADIWSVGCVVVEMASGKRPWAQFDSNFQIMFKVGMGEKPQAPESLSQEGHDFIDHCLQHDPKRRLTAVELLEHNFCKYGRDECSSEQLQMQVRGSFRRNVATSSS

Target ORF information:

RefSeq Version	NM_169833.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Mekk1, transcript variant B (Mekk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169833.2

ORF Insert Sequence:

ATGTCAAACA GGCGAAGAGT GCGAACAATT GATTATCTGG CGCTGCAGCA GAGTTTGCGT 
TTGCAAAAGA CACCGGCAGC AACAACAAAT GCAGAGGAGC AGGTGGCAAG GGAGGAGGAG 
AATGGCAATG GGCATCATAG TACCGTTACA GCTGAAACGC CACCCACACC ACCCATCCCG 
CCCATTCCGC CCATACGATT ACGACGCGAG CAGAGCGTCG AGGAGGATGT TGCAAGAGTT 
GAGTACCGTG TGAAGCAGAC GCCTTCGCGT CCAGTGCAGA TGACTCGAAA TCGGATTGGT 
GCTTTGGAGG AGGACATGCC TCCCGAAGAT GAGCTGGCGG CGCACTATGA GGCCTTCGGC 
ACTACTCCGC CACGTACCAG GCTCAAAATC AAGAACCGCG ACTGGGAACG CAAACAGAAG 
GTGGTCAATG TGACAGCTTC CGCAGACTTA CCGGCCAGTG TGGGTGGCAC CCCCAAAAAG 
ACCAGGACGG CCAGATCCCG CGTCCTGCGG CGCAACACTA TGGACTGCGC CCTGCTCAAC 
GAGATGTTTG TCAACGATGA GAGCAAGCGG ACGGACAAGC GGCTGCAGTC ACTCCTTCGC 
GACTCGGAAC GTGAGATGAA GAACTCGTTG GCCACAACGG CGGTGGCTGC TCCACGAGGT 
AACAGCTTCC ACGAGACAGC TCATCCGCTG GAGTCTCTGG ACCAGATCAT GCCGCTCAAT 
TCGGAGGCAA TGGCGCCGAT TCCACTGCGA ATTGCTTCCA AGGTGGTGGA GAGCTGCAAT 
CGCTATCGCT GCGTGTCCTC ACGTCCCATT GGGTATCGCT CCTCGGCGCC GCCGTTGGCT 
TTCGCTGCCC AACTTCCCGC TGGAATGCTA AGGAGTGATG GAAGAGCAAC ACCTGGGTTG 
GGGAAAAGAA AGGACTTCCA CGAAACGTTT GCCAATCTAA TTAAGCTCGG TAGCGTAGAT 
CGACAGGACG CCAAACTATC ACAGGAGGAG CACACCTGGC AGACTGAGCT GAAGGATCTC 
ATCTGGCTAG AGCTACAGGC CTGGCAAGCA GACCGAACTG TGGAGCAGCA GGACAAGTAC 
CTTTTCGAAG CGCGTCAGGG AGTCTCCGAT CTGCTCACCC ACATTATAAA CTACAAGTTC 
CAGCCGCGTT ATCGCCGCGA GCCGAGTCTG ATAAGCTTGG ATAGTGGCAC TCATTCCGAT 
AGCAATTCTA ATGCCAGCTC TCCACTGCCC AGCAAGATGT GCCAAGGATG CATGTCGCTG 
TACTGCAAGG ATTGCATGGA TCATCAGGAG TTGGCGCTGC GAGAAGTGGA GGGTATGCTG 
ACGCGCCTGG AAGCTGCAGA GGCACTCTAC CCTTCCTCAC AGGCCATGGG CGCCCTGCAT 
CCCATTTACA AATCACAGAG CTTTGTAGGG CGCATCAAGT CCATGTGCTT ATGGTATAAC 
ATTACCAAGC AGAACAAGTT GAAGCTCAGT ATTTTAGGAA AAATTCTGGC AAGACTGCAG 
GATGAGAAGT TCTCTTGGCC CGTGTGCACT TCCTCCTACA TCGCCACCGA CAGCGGTAGT 
TCATCAGCAT CGGGTGTAGA GAACGATGAT TCGGCTGTTA ACTCAATGGA TTCATCAAAG 
CCGCCCAGTA TGGCCGGATC AGCCTCGCGA AAGGGTGTGA CACCGTGCCA CAAAGTGCAA 
TTCATGTTAA ACGACGCCAC CCACGTGCCA GGCGAGACAT CAAGTAGCAA TGAATCCACC 
TCCACGGAGG TAAGCCAGTG GTCCAGCGAG TGCTCTCACA GCCATATGCG TAAGGGATCC 
ATGCACGACA TCAACATTTT CAGTGTCGAG CCATTGGGTA CGTGCAGCAG CAATGGGACA 
GGAGAGCAAT CCACTGGGCT GTATCGCAAG TTCATTGAAA ATGTGCTCAA AAGTCGAGGA 
CTGGCCAAGT CGCTGGCTTT TCTACACAAG CTGCACAACG TGGCATTGTA CAAGGCGCAC 
ATAGCTCTGG AAAAACCCGG AGCTGAAGAC TTCGATTACG AGTCGGATGC AGAGTCCATT 
GAAGAGGACG TACCAAGGCT AGATCCTGAA ATCAGCCGCG AACAGGTTGT GGAGCTGCGC 
ACCTACGGCT ACTGGAGCGA GGAGGCACAA TCCATAAACC TGCCGTCGTA CATTCCCACC 
TTTGTATTTC TGAGCGGAAT TCCGTTGCAG TTCATGCATG AGTTCTTGCG CATGCGTCTC 
GAAACCAGAC CCGTTCGACC CAATCCACTG AGCCTGGAAC AGTTGATGAA GGAGCTTAGG 
GAGGGCTTGA CCCTAGCCTT GACCCATCGG GAGCGATACC AGCGGCACAT AACCACGGCG 
CTGGTGGAGA ATGAGGCGGA ACTGGGCAGT TATATTAGCA TTTTAAACCA CTACGATGCT 
ACGGTGCGAA AGACTTTCGA GCTGTATTTG GAGTACATCG ATCAACTAGT GCTGGTTGCC 
GTTCCGGAAG GCAACCAGAA GTCCGTCCTT GAGAAGGAAT GGATGTTTAC CAAGCTTATC 
AGTCCCATGA TAAAGGGCAT GCACACGTTA GCATCTCAAA AATTCTGTGG CATAATCAGC 
AAACTTTTGC GGAGCATTTC CGAACGCCTG GTGAAGCGCA CCGTTGAGCT AGATCAACAG 
ATCGATGGCA CCGCCGATAC CGACGACAAC GAGGAGGTTA AATGGCAGCT GCTAACCATT 
TGCAGAGAGA CACAATCGCT GCTCACAGTC GAACGGGAGC GCTCCATTAA GGTGCTCTTC 
TTTGCCAAAA CCTTTTGTCG CGACGTCGAG ACGACGGATT TTCATCGCGA GCATTATGAA 
CATGACGTGG CCAACCAGCA GCATGACTTC ATCTGTTCGG ACGTAAAGGC GGCGTTCAAG 
CTCTTGCAGC AGGACGTTCT GCAAGTGCGC AACAAGTTGA CGGCAATAAT TGAAGGAGTG 
CAAAAACGGT GCTGTTTGAG CAACATGCGA GATTTGGATG AGCAAGACAA ACAGGCTGTG 
CTGTCGCGCA CTCGAGAGAT TTTGCATCAG GGATACAAAT TTGGATTTGA ATATCACAAG 
GACGTCATTC GGTTGTTCGA GCAGAAGATT ATGGACCAAA AGGACAGCGG TGCTCACACA 
GTAGATCTTG CCTTAGGAAT TATCGCTTAC GCTAAGATGT GGATGCATTT TGTAATGGAG 
CGTTGCGAAC GTGGACGAGG AATGCGTCCC CGTTGGGCCT CCCAGGGTCT GGAGTTTCTG 
ATTCTTGCCT GTGATCCACA AATCACCCAG CACTTGGACG ATGACGAGTT TGAGGCGCTA 
AAGCAGCAAA TGGATCGCTG TATTTCGCAC GTGATTGGCA TCACCTCGGA ACCCGAAAAG 
GTCGCCAAGA AAAAGGCTTC GCCGCGCACT CGCAAGACTT CATCGCCGGC CACCTCGCGT 
TCCCGGACAC CAACACGGAC TCCAATGTCC GCTGGCATGG TCCTTAATCC GAATACGCCG 
CCACTACAGT CGCCACCGTA CAACAAGTTA CTTCATCCGC AGTTCAGTTT AAAGGAGGAT 
GTGTCAGGAA CTTCGTACAG TCCCGTTGAC AGTTCAGACT ATGTTGACAC TCCGTGCCAA 
AGGAGTGCCA ACGGCGAGCT GCGTCTGCTG GTGCCCCAGA CGCCGCCGAC TCCAGCATCT 
CCCGGAAAGA GCAGCCTCGA AAGTACACCG CTGGCCTTGC GGCAGGAACG TGTTAGAGAT 
GCCGTCAACC GTTTGGATAT GGATCTAGAG GACGGGCTAC GCGAACGAAG GTTGATTGGG 
CAGGTCAAGT CTCTTAATTC CAGCGACAAG GTGCATATAA GGGCGCGTAG CGTTCATTTC 
CGCTGGCATC GTGGCATTAA GATCGGCCAG GGACGATTCG GCAAGGTATA CACGGCGGTG 
AACAATAACA CGGGAGAGCT GATGGCCATG AAAGAGATCG CAATCCAGCC GGGAGAGACG 
CGAGCACTCA AGAATGTGGC CGAAGAGCTA AAGATCTTGG AAGGAATAAA GCACAAAAAC 
CTGGTGCGTT ACTATGGCAT CGAGGTGCAC CGCGAAGAGC TGCTTATTTT CATGGAGCTG 
TGCTCTGAGG GCACCCTCGA GTCACTGGTG GAGCTGACTG GTAATCTGCC GGAGGCGCTT 
ACGCGACGTT TCACCGCCCA GCTGTTGTCG GGCGTGTCTG AGCTGCACAA GCATGGCATT 
GTGCATCGCG ACATCAAGAC TGCTAACATA TTCCTCGTGG ACGGCAGCAA CAGCCTAAAA 
CTGGGCGATT TCGGATCAGC AGTGAAGATC CAAGCGCACA CCACTGTGCC CGGTGAGCTG 
CAGGGCTATG TAGGCACGCA GGCCTATATG GCGCCGGAGG TTTTCACAAA GACCAACAGC 
GATGGCCATG GCAGGGCGGC CGATATCTGG TCGGTGGGCT GTGTGGTTGT CGAGATGGCC 
TCGGGCAAGC GTCCTTGGGC CCAGTTTGAT TCCAACTTCC AGATCATGTT CAAAGTGGGC 
ATGGGCGAGA AGCCGCAGGC ACCGGAGAGC CTATCCCAGG AGGGTCACGA CTTCATCGAT 
CATTGTCTGC AGCACGATCC CAAGAGGCGT TTAACGGCAG TGGAACTGTT GGAGCACAAT 
TTCTGCAAGT ACGGTCGAGA CGAGTGCAGC AGTGAGCAGT TGCAGATGCA GGTGCGAGGC 
TCTTTCCGGC GAAATGTGGC GACCAGCAGC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mekk1 ( NM_142493.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650750.4 Drosophila melanogaster Mekk1, transcript variant D (Mekk1), mRNA.

Price & Availability↑

CloneID	OFa05342
Clone ID Related Accession (Same CDS sequence)	NM_142493.3
Accession Version	NM_142493.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Mekk1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_142493.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTCGAA ATCGGATTGG TGCTTTGGAG GAGGACATGC CTCCCGAAGA TGAGCTGGCG 
GCGCACTATG AGGCCTTCGG CACTACTCCG CCACGTACCA GGCTCAAAAT CAAGAACCGC 
GACTGGGAAC GCAAACAGAA GGTGGTCAAT GTGACAGCTT CCGCAGACTT ACCGGCCAGT 
GTGGGTGGCA CCCCCAAAAA GACCAGGACG GCCAGATCCC GCGTCCTGCG GCGCAACACT 
ATGGACTGCG CCCTGCTCAA CGAGATGTTT GTCAACGATG AGAGCAAGCG GACGGACAAG 
CGGCTGCAGT CACTCCTTCG CGACTCGGAA CGTGAGATGA AGAACTCGTT GGCCACAACG 
GCGGTGGCTG CTCCACGAGG TAACAGCTTC CACGAGACAG CTCATCCGCT GGAGTCTCTG 
GACCAGATCA TGCCGCTCAA TTCGGAGGCA ATGGCGCCGA TTCCACTGCG AATTGCTTCC 
AAGGTGGTGG AGAGCTGCAA TCGCTATCGC TGCGTGTCCT CACGTCCCAT TGGGTATCGC 
TCCTCGGCGC CGCCGTTGGC TTTCGCTGCC CAACTTCCCG CTGGAATGCT AAGGAGTGAT 
GGAAGAGCAA CACCTGGGTT GGGGAAAAGA AAGGACTTCC ACGAAACGTT TGCCAATCTA 
ATTAAGCTCG GTAGCGTAGA TCGACAGGAC GCCAAACTAT CACAGGAGGA GCACACCTGG 
CAGACTGAGC TGAAGGATCT CATCTGGCTA GAGCTACAGG CCTGGCAAGC AGACCGAACT 
GTGGAGCAGC AGGACAAGTA CCTTTTCGAA GCGCGTCAGG GAGTCTCCGA TCTGCTCACC 
CACATTATAA ACTACAAGTT CCAGCCGCGT TATCGCCGCG AGCCGAGTCT GATAAGCTTG 
GATAGTGGCA CTCATTCCGA TAGCAATTCT AATGCCAGCT CTCCACTGCC CAGCAAGATG 
TGCCAAGGAT GCATGTCGCT GTACTGCAAG GATTGCATGG ATCATCAGGA GTTGGCGCTG 
CGAGAAGTGG AGGGTATGCT GACGCGCCTG GAAGCTGCAG AGGCACTCTA CCCTTCCTCA 
CAGGCCATGG GCGCCCTGCA TCCCATTTAC AAATCACAGA GCTTTGTAGG GCGCATCAAG 
TCCATGTGCT TATGGTATAA CATTACCAAG CAGAACAAGT TGAAGCTCAG TATTTTAGGA 
AAAATTCTGG CAAGACTGCA GGATGAGAAG TTCTCTTGGC CCGTGTGCAC TTCCTCCTAC 
ATCGCCACCG ACAGCGGTAG TTCATCAGCA TCGGGTGTAG AGAACGATGA TTCGGCTGTT 
AACTCAATGG ATTCATCAAA GCCGCCCAGT ATGGCCGGAT CAGCCTCGCG AAAGGGTGTG 
ACACCGTGCC ACAAAGTGCA ATTCATGTTA AACGACGCCA CCCACGTGCC AGGCGAGACA 
TCAAGTAGCA ATGAATCCAC CTCCACGGAG GTAAGCCAGT GGTCCAGCGA GTGCTCTCAC 
AGCCATATGC GTAAGGGATC CATGCACGAC ATCAACATTT TCAGTGTCGA GCCATTGGGT 
ACGTGCAGCA GCAATGGGAC AGGAGAGCAA TCCACTGGGC TGTATCGCAA GTTCATTGAA 
AATGTGCTCA AAAGTCGAGG ACTGGCCAAG TCGCTGGCTT TTCTACACAA GCTGCACAAC 
GTGGCATTGT ACAAGGCGCA CATAGCTCTG GAAAAACCCG GAGCTGAAGA CTTCGATTAC 
GAGTCGGATG CAGAGTCCAT TGAAGAGGAC GTACCAAGGC TAGATCCTGA AATCAGCCGC 
GAACAGGTTG TGGAGCTGCG CACCTACGGC TACTGGAGCG AGGAGGCACA ATCCATAAAC 
CTGCCGTCGT ACATTCCCAC CTTTGTATTT CTGAGCGGAA TTCCGTTGCA GTTCATGCAT 
GAGTTCTTGC GCATGCGTCT CGAAACCAGA CCCGTTCGAC CCAATCCACT GAGCCTGGAA 
CAGTTGATGA AGGAGCTTAG GGAGGGCTTG ACCCTAGCCT TGACCCATCG GGAGCGATAC 
CAGCGGCACA TAACCACGGC GCTGGTGGAG AATGAGGCGG AACTGGGCAG TTATATTAGC 
ATTTTAAACC ACTACGATGC TACGGTGCGA AAGACTTTCG AGCTGTATTT GGAGTACATC 
GATCAACTAG TGCTGGTTGC CGTTCCGGAA GGCAACCAGA AGTCCGTCCT TGAGAAGGAA 
TGGATGTTTA CCAAGCTTAT CAGTCCCATG ATAAAGGGCA TGCACACGTT AGCATCTCAA 
AAATTCTGTG GCATAATCAG CAAACTTTTG CGGAGCATTT CCGAACGCCT GGTGAAGCGC 
ACCGTTGAGC TAGATCAACA GATCGATGGC ACCGCCGATA CCGACGACAA CGAGGAGGTT 
AAATGGCAGC TGCTAACCAT TTGCAGAGAG ACACAATCGC TGCTCACAGT CGAACGGGAG 
CGCTCCATTA AGGTGCTCTT CTTTGCCAAA ACCTTTTGTC GCGACGTCGA GACGACGGAT 
TTTCATCGCG AGCATTATGA ACATGACGTG GCCAACCAGC AGCATGACTT CATCTGTTCG 
GACGTAAAGG CGGCGTTCAA GCTCTTGCAG CAGGACGTTC TGCAAGTGCG CAACAAGTTG 
ACGGCAATAA TTGAAGGAGT GCAAAAACGG TGCTGTTTGA GCAACATGCG AGATTTGGAT 
GAGCAAGACA AACAGGCTGT GCTGTCGCGC ACTCGAGAGA TTTTGCATCA GGGATACAAA 
TTTGGATTTG AATATCACAA GGACGTCATT CGGTTGTTCG AGCAGAAGAT TATGGACCAA 
AAGGACAGCG GTGCTCACAC AGTAGATCTT GCCTTAGGAA TTATCGCTTA CGCTAAGATG 
TGGATGCATT TTGTAATGGA GCGTTGCGAA CGTGGACGAG GAATGCGTCC CCGTTGGGCC 
TCCCAGGGTC TGGAGTTTCT GATTCTTGCC TGTGATCCAC AAATCACCCA GCACTTGGAC 
GATGACGAGT TTGAGGCGCT AAAGCAGCAA ATGGATCGCT GTATTTCGCA CGTGATTGGC 
ATCACCTCGG AACCCGAAAA GGTCGCCAAG AAAAAGGCTT CGCCGCGCAC TCGCAAGACT 
TCATCGCCGG CCACCTCGCG TTCCCGGACA CCAACACGGA CTCCAATGTC CGCTGGCATG 
GTCCTTAATC CGAATACGCC GCCACTACAG TCGCCACCGT ACAACAAGTT ACTTCATCCG 
CAGTTCAGTT TAAAGGAGGA TGTGTCAGGA ACTTCGTACA GTCCCGTTGA CAGTTCAGAC 
TATGTTGACA CTCCGTGCCA AAGGAGTGCC AACGGCGAGC TGCGTCTGCT GGTGCCCCAG 
ACGCCGCCGA CTCCAGCATC TCCCGGAAAG AGCAGCCTCG AAAGTACACC GCTGGCCTTG 
CGGCAGGAAC GTGTTAGAGA TGCCGTCAAC CGTTTGGATA TGGATCTAGA GGACGGGCTA 
CGCGAACGAA GGTTGATTGG GCAGGTCAAG TCTCTTAATT CCAGCGACAA GGTGCATATA 
AGGGCGCGTA GCGTTCATTT CCGCTGGCAT CGTGGCATTA AGATCGGCCA GGGACGATTC 
GGCAAGGTAT ACACGGCGGT GAACAATAAC ACGGGAGAGC TGATGGCCAT GAAAGAGATC 
GCAATCCAGC CGGGAGAGAC GCGAGCACTC AAGAATGTGG CCGAAGAGCT AAAGATCTTG 
GAAGGAATAA AGCACAAAAA CCTGGTGCGT TACTATGGCA TCGAGGTGCA CCGCGAAGAG 
CTGCTTATTT TCATGGAGCT GTGCTCTGAG GGCACCCTCG AGTCACTGGT GGAGCTGACT 
GGTAATCTGC CGGAGGCGCT TACGCGACGT TTCACCGCCC AGCTGTTGTC GGGCGTGTCT 
GAGCTGCACA AGCATGGCAT TGTGCATCGC GACATCAAGA CTGCTAACAT ATTCCTCGTG 
GACGGCAGCA ACAGCCTAAA ACTGGGCGAT TTCGGATCAG CAGTGAAGAT CCAAGCGCAC 
ACCACTGTGC CCGGTGAGCT GCAGGGCTAT GTAGGCACGC AGGCCTATAT GGCGCCGGAG 
GTTTTCACAA AGACCAACAG CGATGGCCAT GGCAGGGCGG CCGATATCTG GTCGGTGGGC 
TGTGTGGTTG TCGAGATGGC CTCGGGCAAG CGTCCTTGGG CCCAGTTTGA TTCCAACTTC 
CAGATCATGT TCAAAGTGGG CATGGGCGAG AAGCCGCAGG CACCGGAGAG CCTATCCCAG 
GAGGGTCACG ACTTCATCGA TCATTGTCTG CAGCACGATC CCAAGAGGCG TTTAACGGCA 
GTGGAACTGT TGGAGCACAA TTTCTGCAAG TACGGTCGAG ACGAGTGCAG CAGTGAGCAG 
TTGCAGATGC AGGTGCGAGG CTCTTTCCGG CGAAATGTGG CGACCAGCAG CAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650750.4
CDS	844..5280
Translation	MTRNRIGALEEDMPPEDELAAHYEAFGTTPPRTRLKIKNRDWERKQKVVNVTASADLPASVGGTPKKTRTARSRVLRRNTMDCALLNEMFVNDESKRTDKRLQSLLRDSEREMKNSLATTAVAAPRGNSFHETAHPLESLDQIMPLNSEAMAPIPLRIASKVVESCNRYRCVSSRPIGYRSSAPPLAFAAQLPAGMLRSDGRATPGLGKRKDFHETFANLIKLGSVDRQDAKLSQEEHTWQTELKDLIWLELQAWQADRTVEQQDKYLFEARQGVSDLLTHIINYKFQPRYRREPSLISLDSGTHSDSNSNASSPLPSKMCQGCMSLYCKDCMDHQELALREVEGMLTRLEAAEALYPSSQAMGALHPIYKSQSFVGRIKSMCLWYNITKQNKLKLSILGKILARLQDEKFSWPVCTSSYIATDSGSSSASGVENDDSAVNSMDSSKPPSMAGSASRKGVTPCHKVQFMLNDATHVPGETSSSNESTSTEVSQWSSECSHSHMRKGSMHDINIFSVEPLGTCSSNGTGEQSTGLYRKFIENVLKSRGLAKSLAFLHKLHNVALYKAHIALEKPGAEDFDYESDAESIEEDVPRLDPEISREQVVELRTYGYWSEEAQSINLPSYIPTFVFLSGIPLQFMHEFLRMRLETRPVRPNPLSLEQLMKELREGLTLALTHRERYQRHITTALVENEAELGSYISILNHYDATVRKTFELYLEYIDQLVLVAVPEGNQKSVLEKEWMFTKLISPMIKGMHTLASQKFCGIISKLLRSISERLVKRTVELDQQIDGTADTDDNEEVKWQLLTICRETQSLLTVERERSIKVLFFAKTFCRDVETTDFHREHYEHDVANQQHDFICSDVKAAFKLLQQDVLQVRNKLTAIIEGVQKRCCLSNMRDLDEQDKQAVLSRTREILHQGYKFGFEYHKDVIRLFEQKIMDQKDSGAHTVDLALGIIAYAKMWMHFVMERCERGRGMRPRWASQGLEFLILACDPQITQHLDDDEFEALKQQMDRCISHVIGITSEPEKVAKKKASPRTRKTSSPATSRSRTPTRTPMSAGMVLNPNTPPLQSPPYNKLLHPQFSLKEDVSGTSYSPVDSSDYVDTPCQRSANGELRLLVPQTPPTPASPGKSSLESTPLALRQERVRDAVNRLDMDLEDGLRERRLIGQVKSLNSSDKVHIRARSVHFRWHRGIKIGQGRFGKVYTAVNNNTGELMAMKEIAIQPGETRALKNVAEELKILEGIKHKNLVRYYGIEVHREELLIFMELCSEGTLESLVELTGNLPEALTRRFTAQLLSGVSELHKHGIVHRDIKTANIFLVDGSNSLKLGDFGSAVKIQAHTTVPGELQGYVGTQAYMAPEVFTKTNSDGHGRAADIWSVGCVVVEMASGKRPWAQFDSNFQIMFKVGMGEKPQAPESLSQEGHDFIDHCLQHDPKRRLTAVELLEHNFCKYGRDECSSEQLQMQVRGSFRRNVATSSS

Target ORF information:

RefSeq Version	NM_142493.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Mekk1, transcript variant D (Mekk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142493.3

ORF Insert Sequence:

ATGACTCGAA ATCGGATTGG TGCTTTGGAG GAGGACATGC CTCCCGAAGA TGAGCTGGCG 
GCGCACTATG AGGCCTTCGG CACTACTCCG CCACGTACCA GGCTCAAAAT CAAGAACCGC 
GACTGGGAAC GCAAACAGAA GGTGGTCAAT GTGACAGCTT CCGCAGACTT ACCGGCCAGT 
GTGGGTGGCA CCCCCAAAAA GACCAGGACG GCCAGATCCC GCGTCCTGCG GCGCAACACT 
ATGGACTGCG CCCTGCTCAA CGAGATGTTT GTCAACGATG AGAGCAAGCG GACGGACAAG 
CGGCTGCAGT CACTCCTTCG CGACTCGGAA CGTGAGATGA AGAACTCGTT GGCCACAACG 
GCGGTGGCTG CTCCACGAGG TAACAGCTTC CACGAGACAG CTCATCCGCT GGAGTCTCTG 
GACCAGATCA TGCCGCTCAA TTCGGAGGCA ATGGCGCCGA TTCCACTGCG AATTGCTTCC 
AAGGTGGTGG AGAGCTGCAA TCGCTATCGC TGCGTGTCCT CACGTCCCAT TGGGTATCGC 
TCCTCGGCGC CGCCGTTGGC TTTCGCTGCC CAACTTCCCG CTGGAATGCT AAGGAGTGAT 
GGAAGAGCAA CACCTGGGTT GGGGAAAAGA AAGGACTTCC ACGAAACGTT TGCCAATCTA 
ATTAAGCTCG GTAGCGTAGA TCGACAGGAC GCCAAACTAT CACAGGAGGA GCACACCTGG 
CAGACTGAGC TGAAGGATCT CATCTGGCTA GAGCTACAGG CCTGGCAAGC AGACCGAACT 
GTGGAGCAGC AGGACAAGTA CCTTTTCGAA GCGCGTCAGG GAGTCTCCGA TCTGCTCACC 
CACATTATAA ACTACAAGTT CCAGCCGCGT TATCGCCGCG AGCCGAGTCT GATAAGCTTG 
GATAGTGGCA CTCATTCCGA TAGCAATTCT AATGCCAGCT CTCCACTGCC CAGCAAGATG 
TGCCAAGGAT GCATGTCGCT GTACTGCAAG GATTGCATGG ATCATCAGGA GTTGGCGCTG 
CGAGAAGTGG AGGGTATGCT GACGCGCCTG GAAGCTGCAG AGGCACTCTA CCCTTCCTCA 
CAGGCCATGG GCGCCCTGCA TCCCATTTAC AAATCACAGA GCTTTGTAGG GCGCATCAAG 
TCCATGTGCT TATGGTATAA CATTACCAAG CAGAACAAGT TGAAGCTCAG TATTTTAGGA 
AAAATTCTGG CAAGACTGCA GGATGAGAAG TTCTCTTGGC CCGTGTGCAC TTCCTCCTAC 
ATCGCCACCG ACAGCGGTAG TTCATCAGCA TCGGGTGTAG AGAACGATGA TTCGGCTGTT 
AACTCAATGG ATTCATCAAA GCCGCCCAGT ATGGCCGGAT CAGCCTCGCG AAAGGGTGTG 
ACACCGTGCC ACAAAGTGCA ATTCATGTTA AACGACGCCA CCCACGTGCC AGGCGAGACA 
TCAAGTAGCA ATGAATCCAC CTCCACGGAG GTAAGCCAGT GGTCCAGCGA GTGCTCTCAC 
AGCCATATGC GTAAGGGATC CATGCACGAC ATCAACATTT TCAGTGTCGA GCCATTGGGT 
ACGTGCAGCA GCAATGGGAC AGGAGAGCAA TCCACTGGGC TGTATCGCAA GTTCATTGAA 
AATGTGCTCA AAAGTCGAGG ACTGGCCAAG TCGCTGGCTT TTCTACACAA GCTGCACAAC 
GTGGCATTGT ACAAGGCGCA CATAGCTCTG GAAAAACCCG GAGCTGAAGA CTTCGATTAC 
GAGTCGGATG CAGAGTCCAT TGAAGAGGAC GTACCAAGGC TAGATCCTGA AATCAGCCGC 
GAACAGGTTG TGGAGCTGCG CACCTACGGC TACTGGAGCG AGGAGGCACA ATCCATAAAC 
CTGCCGTCGT ACATTCCCAC CTTTGTATTT CTGAGCGGAA TTCCGTTGCA GTTCATGCAT 
GAGTTCTTGC GCATGCGTCT CGAAACCAGA CCCGTTCGAC CCAATCCACT GAGCCTGGAA 
CAGTTGATGA AGGAGCTTAG GGAGGGCTTG ACCCTAGCCT TGACCCATCG GGAGCGATAC 
CAGCGGCACA TAACCACGGC GCTGGTGGAG AATGAGGCGG AACTGGGCAG TTATATTAGC 
ATTTTAAACC ACTACGATGC TACGGTGCGA AAGACTTTCG AGCTGTATTT GGAGTACATC 
GATCAACTAG TGCTGGTTGC CGTTCCGGAA GGCAACCAGA AGTCCGTCCT TGAGAAGGAA 
TGGATGTTTA CCAAGCTTAT CAGTCCCATG ATAAAGGGCA TGCACACGTT AGCATCTCAA 
AAATTCTGTG GCATAATCAG CAAACTTTTG CGGAGCATTT CCGAACGCCT GGTGAAGCGC 
ACCGTTGAGC TAGATCAACA GATCGATGGC ACCGCCGATA CCGACGACAA CGAGGAGGTT 
AAATGGCAGC TGCTAACCAT TTGCAGAGAG ACACAATCGC TGCTCACAGT CGAACGGGAG 
CGCTCCATTA AGGTGCTCTT CTTTGCCAAA ACCTTTTGTC GCGACGTCGA GACGACGGAT 
TTTCATCGCG AGCATTATGA ACATGACGTG GCCAACCAGC AGCATGACTT CATCTGTTCG 
GACGTAAAGG CGGCGTTCAA GCTCTTGCAG CAGGACGTTC TGCAAGTGCG CAACAAGTTG 
ACGGCAATAA TTGAAGGAGT GCAAAAACGG TGCTGTTTGA GCAACATGCG AGATTTGGAT 
GAGCAAGACA AACAGGCTGT GCTGTCGCGC ACTCGAGAGA TTTTGCATCA GGGATACAAA 
TTTGGATTTG AATATCACAA GGACGTCATT CGGTTGTTCG AGCAGAAGAT TATGGACCAA 
AAGGACAGCG GTGCTCACAC AGTAGATCTT GCCTTAGGAA TTATCGCTTA CGCTAAGATG 
TGGATGCATT TTGTAATGGA GCGTTGCGAA CGTGGACGAG GAATGCGTCC CCGTTGGGCC 
TCCCAGGGTC TGGAGTTTCT GATTCTTGCC TGTGATCCAC AAATCACCCA GCACTTGGAC 
GATGACGAGT TTGAGGCGCT AAAGCAGCAA ATGGATCGCT GTATTTCGCA CGTGATTGGC 
ATCACCTCGG AACCCGAAAA GGTCGCCAAG AAAAAGGCTT CGCCGCGCAC TCGCAAGACT 
TCATCGCCGG CCACCTCGCG TTCCCGGACA CCAACACGGA CTCCAATGTC CGCTGGCATG 
GTCCTTAATC CGAATACGCC GCCACTACAG TCGCCACCGT ACAACAAGTT ACTTCATCCG 
CAGTTCAGTT TAAAGGAGGA TGTGTCAGGA ACTTCGTACA GTCCCGTTGA CAGTTCAGAC 
TATGTTGACA CTCCGTGCCA AAGGAGTGCC AACGGCGAGC TGCGTCTGCT GGTGCCCCAG 
ACGCCGCCGA CTCCAGCATC TCCCGGAAAG AGCAGCCTCG AAAGTACACC GCTGGCCTTG 
CGGCAGGAAC GTGTTAGAGA TGCCGTCAAC CGTTTGGATA TGGATCTAGA GGACGGGCTA 
CGCGAACGAA GGTTGATTGG GCAGGTCAAG TCTCTTAATT CCAGCGACAA GGTGCATATA 
AGGGCGCGTA GCGTTCATTT CCGCTGGCAT CGTGGCATTA AGATCGGCCA GGGACGATTC 
GGCAAGGTAT ACACGGCGGT GAACAATAAC ACGGGAGAGC TGATGGCCAT GAAAGAGATC 
GCAATCCAGC CGGGAGAGAC GCGAGCACTC AAGAATGTGG CCGAAGAGCT AAAGATCTTG 
GAAGGAATAA AGCACAAAAA CCTGGTGCGT TACTATGGCA TCGAGGTGCA CCGCGAAGAG 
CTGCTTATTT TCATGGAGCT GTGCTCTGAG GGCACCCTCG AGTCACTGGT GGAGCTGACT 
GGTAATCTGC CGGAGGCGCT TACGCGACGT TTCACCGCCC AGCTGTTGTC GGGCGTGTCT 
GAGCTGCACA AGCATGGCAT TGTGCATCGC GACATCAAGA CTGCTAACAT ATTCCTCGTG 
GACGGCAGCA ACAGCCTAAA ACTGGGCGAT TTCGGATCAG CAGTGAAGAT CCAAGCGCAC 
ACCACTGTGC CCGGTGAGCT GCAGGGCTAT GTAGGCACGC AGGCCTATAT GGCGCCGGAG 
GTTTTCACAA AGACCAACAG CGATGGCCAT GGCAGGGCGG CCGATATCTG GTCGGTGGGC 
TGTGTGGTTG TCGAGATGGC CTCGGGCAAG CGTCCTTGGG CCCAGTTTGA TTCCAACTTC 
CAGATCATGT TCAAAGTGGG CATGGGCGAG AAGCCGCAGG CACCGGAGAG CCTATCCCAG 
GAGGGTCACG ACTTCATCGA TCATTGTCTG CAGCACGATC CCAAGAGGCG TTTAACGGCA 
GTGGAACTGT TGGAGCACAA TTTCTGCAAG TACGGTCGAG ACGAGTGCAG CAGTGAGCAG 
TTGCAGATGC AGGTGCGAGG CTCTTTCCGG CGAAATGTGG CGACCAGCAG CAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mekk1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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