Cpsf73 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cpsf73
Entrez Gene ID	42240
Full Name	Cleavage and polyadenylation specificity factor 73
Synonyms	CG7698,CPSF-73,CPSF73,Dmel\CG7698,cpsf-73,dCPSF-73
General protein information	Preferred Names Cleavage and polyadenylation specificity factor 73 Names CG7698 gene product from transcript CG7698-RA CG7698-PA Cpsf73-PA cleavage and polyadenylation specificity factor 73 cleavage and polyadenylation specificity factor, 73kD-subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91B8-91B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_142481.3	NP_650738.1	cleavage and polyadenylation specificity factor 73

Pathways from BioSystems

Homologies

Homo sapiens (human)	CPSF3	NP_057291.1
Caenorhabditis elegans (roundworm)	cpsf-3	NP_502553.2
Oryza sativa (rice)	Os03g0852900	NP_001051928.1
Canis lupus familiaris (dog)	CPSF3	XP_005630164.1
Mus musculus (house mouse)	Cpsf3	NP_061283.2
Danio rerio (zebrafish)	cpsf3	NP_001003836.1
Kluyveromyces lactis	KLLA0C04598g	XP_452404.1
Eremothecium gossypii	AGOS_AGR279C	NP_986945.2
Magnaporthe oryzae (rice blast fungus)	MGG_01804	XP_003714815.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001224	XP_321933.5
Saccharomyces cerevisiae (baker's yeast)	YSH1	NP_013379.1
Schizosaccharomyces pombe (fission yeast)	ysh1	NP_594263.2
Arabidopsis thaliana (thale cress)	CPSF73-I	NP_001031215.1
Pan troglodytes (chimpanzee)	CPSF3	NP_001233296.1
	CPSF3	XP_002799184.1
Bos taurus (cattle)	CPSF3	NP_776709.1
Rattus norvegicus (Norway rat)	Cpsf3	NP_001025201.1
Gallus gallus (chicken)	CPSF3	XP_419942.4
Drosophila melanogaster (fruit fly)	Cpsf73	NP_650738.1
Neurospora crassa	NCU03479	XP_956368.1
Xenopus tropicalis (tropical clawed frog)	cpsf3	NP_001006770.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors propose a model in which the core cleavage complex formation is mediated by CPSF73, CPSF100, and Symplekin C-termini, and the N-terminal region of Symplekin facilitates cotranscriptional 3' end processing of histone mRNAs.
Title: In vivo characterization of the Drosophila mRNA 3' end processing core cleavage complex.

Data demonstrate that Symplekin (CG2097), CPSF73 (CG7698), and CPSF100 (CG1957) are present in a stable complex and interact with histone-specific processing factors.
Title: A core complex of CPSF73, CPSF100, and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs.

The following Cpsf73 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cpsf73 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05328	NM_142481.3 Latest version!	Drosophila melanogaster cleavage and polyadenylation specificity factor 73 (Cpsf73), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cpsf73 ( NM_142481.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650738.1 Drosophila melanogaster cleavage and polyadenylation specificity factor 73 (Cpsf73), mRNA.

Price & Availability↑

CloneID	OFa05328
Clone ID Related Accession (Same CDS sequence)	NM_142481.3
Accession Version	NM_142481.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cleavage and polyadenylation specificity factor 73
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142481.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAGG CAACAGGTGA TGCACGCATG CCAGACGAGG AAAGCGACCT GCTGCAGATC 
AAGCCACTTG GTGCTGGCCA GGAAGTGGGA CGCTCCTGCA TCATGTTGGA ATTTAAGGGC 
AAGAAGATAA TGCTGGACTG CGGAATCCAT CCGGGATTGT CCGGCATGGA TGCCCTGCCC 
TACGTAGATC TAATAGAGGC GGATGAGATT GATCTGCTGT TTATATCACA TTTCCACTTG 
GATCACTGCG GTGCCTTGCC TTGGTTTCTC ATGAAGACCA GCTTTAAAGG CCGCTGCTTT 
ATGACCCATG CCACGAAAGC CATTTACCGA TGGATGCTGT CCGATTACAT CAAGATCAGT 
AACATATCCA CCGAACAGAT GCTATACACG GAAGCCGATC TGGAGGCCTC CATGGAGAAG 
ATCGAGACGA TCAATTTCCA CGAGGAGCGC GATGTGATGG GCGTACGCTT TTGTGCCTAT 
ATTGCTGGTC ATGTTTTGGG TGCCGCCATG TTCATGATCG AGATTGCTGG CATCAAGATC 
TTGTATACTG GTGACTTTTC GCGCCAGGAG GATCGACATT TGATGGCTGC CGAAGTGCCA 
CCGATGAAGC CGGATGTTCT TATCACAGAG TCCACCTACG GCACTCACAT TCACGAGAAA 
AGAGAGGATC GAGAGAATCG ATTTACCTCG CTGGTGCAGA AAATTGTTCA GCAAGGCGGG 
AGATGCTTAA TTCCAGTATT TGCTTTGGGC CGTGCCCAAG AACTGCTACT TATTCTGGAT 
GAATTCTGGT CGCAGAACCC GGATCTGCAC GAGATTCCCA TCTACTATGC CTCCTCTTTG 
GCCAAGAAGT GCATGGCTGT CTACCAGACG TACATCAATG CTATGAACGA TCGAATTCGA 
CGACAGATAG CTGTGAACAA TCCCTTCGTT TTCCGCCACA TTTCCAACCT GAAGGGTATC 
GATCACTTCG AGGATATCGG ACCCTGCGTA ATCATGGCGT CGCCGGGTAT GATGCAGTCG 
GGATTGTCGC GAGAGCTCTT CGAGAGCTGG TGCACAGATC CCAAGAACGG AGTGATTATA 
GCCGGTTACT GCGTGGAGGG CACTTTGGCC AAAGCCGTTC TCTCCGAGCC GGAGGAGATC 
ACCACACTGT CGGGCCAGAA GCTGCCGCTC AACATGTCCG TCGACTACAT ATCGTTTTCG 
GCTCACACGG ACTACCAGCA GACCAGTGAA TTCATCCGCC TACTAAAGCC AACGCATGTC 
GTGCTCGTCC ACGGCGAGCA GAACGAGATG TCGCGTCTGA AGTTGGCCCT GCAGCGGGAA 
TACGAAGCAG ATGCAAGCAC GGATATAAAA TTCTACAACC CGCGAAACAC ACATGCCGTG 
GATCTTTATT TCCGTGGCGA GAAAACCGCC AAGGTGATGG GAAGCCTCGC AGCCAAGAAC 
TCGGAGGTGG GAAGCAAACT CTCAGGTGTT CTGGTTAAGC GCGACTTTAA GTACCACCTC 
TTGGCTCCTT CTGATCTGGG CAAATACACG GATATGAGCA TGTCTGTTGT AACCCAACGC 
CAGTCGATTC CCTGGGGCAG CTCGCTGAGC ACTCTAGAGC TACTGCTGGA TCGCATTGGA 
GCCGGATGCG TTGAGGTGCT GGAGGCGGAG CGAAAGCTGC GCGTGTTCGG CTGCATCGAG 
CTAACTGTGG AGCAGAAAAT CATTGTGATG GAGTGGCAAG CGACGCATGT GAATGATGTG 
TACGCCGATG CCGTTCTTGC CTGCATCATG CAATCGGAGT TGGGCGGAAC AAATCTGAAG 
GGTGCCACAA AGCAAACCAA GTCAGAGGAC TCGCGCTTTC GGGAGTGCCT AATCGAGACG 
CTACAGGACA CCTTCGGCGA CAACTGCGTG CCAAAAATGT TCGAGGGCGA TCTGCTGCCA 
GTGACGGTGA GCGGTAAACG TGCGGAAATA AATCTGGAAA CCCTGGCCAT CAGCTGTGCC 
GAGGATGATG TGCTGCGACA AATGCTCAAT ACGACGGTTC AGAAGTTACA CCAGACCCTA 
GTCTCCGCTC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650738.1
CDS	98..2152
Translation	MTQATGDARMPDEESDLLQIKPLGAGQEVGRSCIMLEFKGKKIMLDCGIHPGLSGMDALPYVDLIEADEIDLLFISHFHLDHCGALPWFLMKTSFKGRCFMTHATKAIYRWMLSDYIKISNISTEQMLYTEADLEASMEKIETINFHEERDVMGVRFCAYIAGHVLGAAMFMIEIAGIKILYTGDFSRQEDRHLMAAEVPPMKPDVLITESTYGTHIHEKREDRENRFTSLVQKIVQQGGRCLIPVFALGRAQELLLILDEFWSQNPDLHEIPIYYASSLAKKCMAVYQTYINAMNDRIRRQIAVNNPFVFRHISNLKGIDHFEDIGPCVIMASPGMMQSGLSRELFESWCTDPKNGVIIAGYCVEGTLAKAVLSEPEEITTLSGQKLPLNMSVDYISFSAHTDYQQTSEFIRLLKPTHVVLVHGEQNEMSRLKLALQREYEADASTDIKFYNPRNTHAVDLYFRGEKTAKVMGSLAAKNSEVGSKLSGVLVKRDFKYHLLAPSDLGKYTDMSMSVVTQRQSIPWGSSLSTLELLLDRIGAGCVEVLEAERKLRVFGCIELTVEQKIIVMEWQATHVNDVYADAVLACIMQSELGGTNLKGATKQTKSEDSRFRECLIETLQDTFGDNCVPKMFEGDLLPVTVSGKRAEINLETLAISCAEDDVLRQMLNTTVQKLHQTLVSAL

Target ORF information:

RefSeq Version	NM_142481.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cleavage and polyadenylation specificity factor 73 (Cpsf73), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142481.3

ORF Insert Sequence:

ATGACGCAGG CAACAGGTGA TGCACGCATG CCAGACGAGG AAAGCGACCT GCTGCAGATC 
AAGCCACTTG GTGCTGGCCA GGAAGTGGGA CGCTCCTGCA TCATGTTGGA ATTTAAGGGC 
AAGAAGATAA TGCTGGACTG CGGAATCCAT CCGGGATTGT CCGGCATGGA TGCCCTGCCC 
TACGTAGATC TAATAGAGGC GGATGAGATT GATCTGCTGT TTATATCACA TTTCCACTTG 
GATCACTGCG GTGCCTTGCC TTGGTTTCTC ATGAAGACCA GCTTTAAAGG CCGCTGCTTT 
ATGACCCATG CCACGAAAGC CATTTACCGA TGGATGCTGT CCGATTACAT CAAGATCAGT 
AACATATCCA CCGAACAGAT GCTATACACG GAAGCCGATC TGGAGGCCTC CATGGAGAAG 
ATCGAGACGA TCAATTTCCA CGAGGAGCGC GATGTGATGG GCGTACGCTT TTGTGCCTAT 
ATTGCTGGTC ATGTTTTGGG TGCCGCCATG TTCATGATCG AGATTGCTGG CATCAAGATC 
TTGTATACTG GTGACTTTTC GCGCCAGGAG GATCGACATT TGATGGCTGC CGAAGTGCCA 
CCGATGAAGC CGGATGTTCT TATCACAGAG TCCACCTACG GCACTCACAT TCACGAGAAA 
AGAGAGGATC GAGAGAATCG ATTTACCTCG CTGGTGCAGA AAATTGTTCA GCAAGGCGGG 
AGATGCTTAA TTCCAGTATT TGCTTTGGGC CGTGCCCAAG AACTGCTACT TATTCTGGAT 
GAATTCTGGT CGCAGAACCC GGATCTGCAC GAGATTCCCA TCTACTATGC CTCCTCTTTG 
GCCAAGAAGT GCATGGCTGT CTACCAGACG TACATCAATG CTATGAACGA TCGAATTCGA 
CGACAGATAG CTGTGAACAA TCCCTTCGTT TTCCGCCACA TTTCCAACCT GAAGGGTATC 
GATCACTTCG AGGATATCGG ACCCTGCGTA ATCATGGCGT CGCCGGGTAT GATGCAGTCG 
GGATTGTCGC GAGAGCTCTT CGAGAGCTGG TGCACAGATC CCAAGAACGG AGTGATTATA 
GCCGGTTACT GCGTGGAGGG CACTTTGGCC AAAGCCGTTC TCTCCGAGCC GGAGGAGATC 
ACCACACTGT CGGGCCAGAA GCTGCCGCTC AACATGTCCG TCGACTACAT ATCGTTTTCG 
GCTCACACGG ACTACCAGCA GACCAGTGAA TTCATCCGCC TACTAAAGCC AACGCATGTC 
GTGCTCGTCC ACGGCGAGCA GAACGAGATG TCGCGTCTGA AGTTGGCCCT GCAGCGGGAA 
TACGAAGCAG ATGCAAGCAC GGATATAAAA TTCTACAACC CGCGAAACAC ACATGCCGTG 
GATCTTTATT TCCGTGGCGA GAAAACCGCC AAGGTGATGG GAAGCCTCGC AGCCAAGAAC 
TCGGAGGTGG GAAGCAAACT CTCAGGTGTT CTGGTTAAGC GCGACTTTAA GTACCACCTC 
TTGGCTCCTT CTGATCTGGG CAAATACACG GATATGAGCA TGTCTGTTGT AACCCAACGC 
CAGTCGATTC CCTGGGGCAG CTCGCTGAGC ACTCTAGAGC TACTGCTGGA TCGCATTGGA 
GCCGGATGCG TTGAGGTGCT GGAGGCGGAG CGAAAGCTGC GCGTGTTCGG CTGCATCGAG 
CTAACTGTGG AGCAGAAAAT CATTGTGATG GAGTGGCAAG CGACGCATGT GAATGATGTG 
TACGCCGATG CCGTTCTTGC CTGCATCATG CAATCGGAGT TGGGCGGAAC AAATCTGAAG 
GGTGCCACAA AGCAAACCAA GTCAGAGGAC TCGCGCTTTC GGGAGTGCCT AATCGAGACG 
CTACAGGACA CCTTCGGCGA CAACTGCGTG CCAAAAATGT TCGAGGGCGA TCTGCTGCCA 
GTGACGGTGA GCGGTAAACG TGCGGAAATA AATCTGGAAA CCCTGGCCAT CAGCTGTGCC 
GAGGATGATG TGCTGCGACA AATGCTCAAT ACGACGGTTC AGAAGTTACA CCAGACCCTA 
GTCTCCGCTC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cpsf73 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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