WRNexo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	WRNexo
Entrez Gene ID	42208
Full Name	WRN exonuclease
Synonyms	CG7670,DmWRNexo,Dmel\CG7670
General protein information	Preferred Names WRN exonuclease Names CG7670 gene product from transcript CG7670-RC CG7670-PA CG7670-PB CG7670-PC WRNexo-PA WRNexo-PB WRNexo-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91A3-91A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_142458.4	NP_650715.3	WRN exonuclease, isoform A
NM_001170175.2	NP_001163646.1	WRN exonuclease, isoform B
NM_001300460.1	NP_001287389.1	WRN exonuclease, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	WRNexo	NP_650715.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000530	XP_565822.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WRNexo is important for recovery following both endogenous and exogenous replication stress; the critical role of WRNexo during replication stress does not depend on exonuclease activity
Title: The Drosophila Werner exonuclease participates in an exonuclease-independent response to replication stress.

DmWRNexo is a 3'-5' exonuclease: phenotypic and biochemical characterization of mutants of the Drosophila orthologue of human WRN exonuclease.
Title: DmWRNexo is a 3'-5' exonuclease: phenotypic and biochemical characterization of mutants of the Drosophila orthologue of human WRN exonuclease.

The Drosophila WRN ortholog demonstrates an important role for WRN exonuclease in maintaining genome stability.
Title: Identification and characterization of a Drosophila ortholog of WRN exonuclease that is required to maintain genome integrity.

The following WRNexo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WRNexo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05299	NM_001170175.2 Latest version!	Drosophila melanogaster WRN exonuclease (WRNexo), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa08501	NM_142458.4 Latest version!	Drosophila melanogaster WRN exonuclease (WRNexo), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa22107	NM_001300460.1 Latest version!	Drosophila melanogaster WRN exonuclease (WRNexo), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WRNexo ( NM_001170175.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163646.1 Drosophila melanogaster WRN exonuclease (WRNexo), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05299
Clone ID Related Accession (Same CDS sequence)	NM_001170175.2
Accession Version	NM_001170175.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	WRN exonuclease, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170175.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAAT ATTTAACAAA AATGCCCATC AAATCAAAAG CAAATGAAGT TCCCAAGGAA 
GAGGCGGGCG TCAAAAAGGA GACTCCCAAA GTGGCAAGGA AAGCAACAAA AAAGGATACT 
CCAAAAGAGT TGAAAGATAA AGAAAATGCT GGAGATGATA ATACGCCAAA GCAAACGAAA 
GGAAGACCCG GTAGACCGGC TGCCAAGCGA AAAAATCTGG ATACTCCAGA TGTGAAGACT 
GAGAAGTTAG CGATGGAGGA AGAAAATCCG CCCAAGCGCC GGAGTTCCAG GCTGACCAGA 
AGCACTCGTT CAATGGCAGA GGATGGGTCT CCATCACCGG AGAAGGAAAA GCCCGAAAAG 
CTTCCCTTTA TTAAATACAA GGGTGCTATA AAGTATTTCA CGGAGAGCCA GGATATTGCG 
GCATCTGCTG ACGATGTTTT GCAATGGGTG GAAAAACAAA AAGATGAGGT AGTGCCCATG 
GCCTTCGACA TGGAGTGGCC CTTCTCTTTC CAAACAGGAC CTGGCAAGTC TGCCGTCATC 
CAGATATGCG TGGATGAAAA GTGCTGCTAC ATATACCAGC TGACTAATGT GAAGAAACTG 
CCTGCGGCCC TGGTGGCCCT TATCAATCAT CCCAAAGTGC GATTGCATGG CGTGAACATA 
AAGAACGATT TCCGAAAGCT GGCACGTGAT TTCCCCGAGG TTACAGCCGA ACCACTGATA 
GAGAAATGCG TGGACTTGGG TCTTTGGTGC AATGAGGTCT GTGAGACCGG CGGGCGCTGG 
AGCTTGGAGC GATTAACCAA CTTTATAGCC AAGAAGGCCA TGGACAAAAG CAAGAAGGTG 
CGCATGAGCA AGTGGCATGT CATTCCGCTG GACGAGAATC AACTAATGTA CGCCGCCATC 
GATGTCTATA TTGGTCAGGT TATCTATCGT GAATTGGAAC GCCGCGAGAA GGTCAAGATT 
AAAAATGAAG AAGAGTTCAA GGAAAAGAAC GGAGATGCTG CCTTTAAAGC AATGAAAGCA 
TTGGGAGAGA CATTTCTGAC CAAGATCAAC GAGGTGACTC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163646.1
CDS	68..1132
Translation	MEKYLTKMPIKSKANEVPKEEAGVKKETPKVARKATKKDTPKELKDKENAGDDNTPKQTKGRPGRPAAKRKNLDTPDVKTEKLAMEEENPPKRRSSRLTRSTRSMAEDGSPSPEKEKPEKLPFIKYKGAIKYFTESQDIAASADDVLQWVEKQKDEVVPMAFDMEWPFSFQTGPGKSAVIQICVDEKCCYIYQLTNVKKLPAALVALINHPKVRLHGVNIKNDFRKLARDFPEVTAEPLIEKCVDLGLWCNEVCETGGRWSLERLTNFIAKKAMDKSKKVRMSKWHVIPLDENQLMYAAIDVYIGQVIYRELERREKVKIKNEEEFKEKNGDAAFKAMKALGETFLTKINEVTL

Target ORF information:

RefSeq Version	NM_001170175.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster WRN exonuclease (WRNexo), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170175.2

ORF Insert Sequence:

ATGGAAAAAT ATTTAACAAA AATGCCCATC AAATCAAAAG CAAATGAAGT TCCCAAGGAA 
GAGGCGGGCG TCAAAAAGGA GACTCCCAAA GTGGCAAGGA AAGCAACAAA AAAGGATACT 
CCAAAAGAGT TGAAAGATAA AGAAAATGCT GGAGATGATA ATACGCCAAA GCAAACGAAA 
GGAAGACCCG GTAGACCGGC TGCCAAGCGA AAAAATCTGG ATACTCCAGA TGTGAAGACT 
GAGAAGTTAG CGATGGAGGA AGAAAATCCG CCCAAGCGCC GGAGTTCCAG GCTGACCAGA 
AGCACTCGTT CAATGGCAGA GGATGGGTCT CCATCACCGG AGAAGGAAAA GCCCGAAAAG 
CTTCCCTTTA TTAAATACAA GGGTGCTATA AAGTATTTCA CGGAGAGCCA GGATATTGCG 
GCATCTGCTG ACGATGTTTT GCAATGGGTG GAAAAACAAA AAGATGAGGT AGTGCCCATG 
GCCTTCGACA TGGAGTGGCC CTTCTCTTTC CAAACAGGAC CTGGCAAGTC TGCCGTCATC 
CAGATATGCG TGGATGAAAA GTGCTGCTAC ATATACCAGC TGACTAATGT GAAGAAACTG 
CCTGCGGCCC TGGTGGCCCT TATCAATCAT CCCAAAGTGC GATTGCATGG CGTGAACATA 
AAGAACGATT TCCGAAAGCT GGCACGTGAT TTCCCCGAGG TTACAGCCGA ACCACTGATA 
GAGAAATGCG TGGACTTGGG TCTTTGGTGC AATGAGGTCT GTGAGACCGG CGGGCGCTGG 
AGCTTGGAGC GATTAACCAA CTTTATAGCC AAGAAGGCCA TGGACAAAAG CAAGAAGGTG 
CGCATGAGCA AGTGGCATGT CATTCCGCTG GACGAGAATC AACTAATGTA CGCCGCCATC 
GATGTCTATA TTGGTCAGGT TATCTATCGT GAATTGGAAC GCCGCGAGAA GGTCAAGATT 
AAAAATGAAG AAGAGTTCAA GGAAAAGAAC GGAGATGCTG CCTTTAAAGC AATGAAAGCA 
TTGGGAGAGA CATTTCTGAC CAAGATCAAC GAGGTGACTC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRNexo ( NM_142458.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650715.3 Drosophila melanogaster WRN exonuclease (WRNexo), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa08501
Clone ID Related Accession (Same CDS sequence)	NM_142458.4
Accession Version	NM_142458.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	WRN exonuclease, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142458.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAAT ATTTAACAAA AATGCCCATC AAATCAAAAG CAAATGAAGT TCCCAAGGAA 
GAGGCGGGCG TCAAAAAGGA GACTCCCAAA GTGGCAAGGA AAGCAACAAA AAAGGATACT 
CCAAAAGAGT TGAAAGATAA AGAAAATGCT GGAGATGATA ATACGCCAAA GCAAACGAAA 
GGAAGACCCG GTAGACCGGC TGCCAAGCGA AAAAATCTGG ATACTCCAGA TGTGACTGAG 
AAGTTAGCGA TGGAGGAAGA AAATCCGCCC AAGCGCCGGA GTTCCAGGCT GACCAGAAGC 
ACTCGTTCAA TGGCAGAGGA TGGGTCTCCA TCACCGGAGA AGGAAAAGCC CGAAAAGCTT 
CCCTTTATTA AATACAAGGG TGCTATAAAG TATTTCACGG AGAGCCAGGA TATTGCGGCA 
TCTGCTGACG ATGTTTTGCA ATGGGTGGAA AAACAAAAAG ATGAGGTAGT GCCCATGGCC 
TTCGACATGG AGTGGCCCTT CTCTTTCCAA ACAGGACCTG GCAAGTCTGC CGTCATCCAG 
ATATGCGTGG ATGAAAAGTG CTGCTACATA TACCAGCTGA CTAATGTGAA GAAACTGCCT 
GCGGCCCTGG TGGCCCTTAT CAATCATCCC AAAGTGCGAT TGCATGGCGT GAACATAAAG 
AACGATTTCC GAAAGCTGGC ACGTGATTTC CCCGAGGTTA CAGCCGAACC ACTGATAGAG 
AAATGCGTGG ACTTGGGTCT TTGGTGCAAT GAGGTCTGTG AGACCGGCGG GCGCTGGAGC 
TTGGAGCGAT TAACCAACTT TATAGCCAAG AAGGCCATGG ACAAAAGCAA GAAGGTGCGC 
ATGAGCAAGT GGCATGTCAT TCCGCTGGAC GAGAATCAAC TAATGTACGC CGCCATCGAT 
GTCTATATTG GTCAGGTTAT CTATCGTGAA TTGGAACGCC GCGAGAAGGT CAAGATTAAA 
AATGAAGAAG AGTTCAAGGA AAAGAACGGA GATGCTGCCT TTAAAGCAAT GAAAGCATTG 
GGAGAGACAT TTCTGACCAA GATCAACGAG GTGACTCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650715.3
CDS	68..1129
Translation	MEKYLTKMPIKSKANEVPKEEAGVKKETPKVARKATKKDTPKELKDKENAGDDNTPKQTKGRPGRPAAKRKNLDTPDVTEKLAMEEENPPKRRSSRLTRSTRSMAEDGSPSPEKEKPEKLPFIKYKGAIKYFTESQDIAASADDVLQWVEKQKDEVVPMAFDMEWPFSFQTGPGKSAVIQICVDEKCCYIYQLTNVKKLPAALVALINHPKVRLHGVNIKNDFRKLARDFPEVTAEPLIEKCVDLGLWCNEVCETGGRWSLERLTNFIAKKAMDKSKKVRMSKWHVIPLDENQLMYAAIDVYIGQVIYRELERREKVKIKNEEEFKEKNGDAAFKAMKALGETFLTKINEVTL

Target ORF information:

RefSeq Version	NM_142458.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster WRN exonuclease (WRNexo), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142458.4

ORF Insert Sequence:

ATGGAAAAAT ATTTAACAAA AATGCCCATC AAATCAAAAG CAAATGAAGT TCCCAAGGAA 
GAGGCGGGCG TCAAAAAGGA GACTCCCAAA GTGGCAAGGA AAGCAACAAA AAAGGATACT 
CCAAAAGAGT TGAAAGATAA AGAAAATGCT GGAGATGATA ATACGCCAAA GCAAACGAAA 
GGAAGACCCG GTAGACCGGC TGCCAAGCGA AAAAATCTGG ATACTCCAGA TGTGACTGAG 
AAGTTAGCGA TGGAGGAAGA AAATCCGCCC AAGCGCCGGA GTTCCAGGCT GACCAGAAGC 
ACTCGTTCAA TGGCAGAGGA TGGGTCTCCA TCACCGGAGA AGGAAAAGCC CGAAAAGCTT 
CCCTTTATTA AATACAAGGG TGCTATAAAG TATTTCACGG AGAGCCAGGA TATTGCGGCA 
TCTGCTGACG ATGTTTTGCA ATGGGTGGAA AAACAAAAAG ATGAGGTAGT GCCCATGGCC 
TTCGACATGG AGTGGCCCTT CTCTTTCCAA ACAGGACCTG GCAAGTCTGC CGTCATCCAG 
ATATGCGTGG ATGAAAAGTG CTGCTACATA TACCAGCTGA CTAATGTGAA GAAACTGCCT 
GCGGCCCTGG TGGCCCTTAT CAATCATCCC AAAGTGCGAT TGCATGGCGT GAACATAAAG 
AACGATTTCC GAAAGCTGGC ACGTGATTTC CCCGAGGTTA CAGCCGAACC ACTGATAGAG 
AAATGCGTGG ACTTGGGTCT TTGGTGCAAT GAGGTCTGTG AGACCGGCGG GCGCTGGAGC 
TTGGAGCGAT TAACCAACTT TATAGCCAAG AAGGCCATGG ACAAAAGCAA GAAGGTGCGC 
ATGAGCAAGT GGCATGTCAT TCCGCTGGAC GAGAATCAAC TAATGTACGC CGCCATCGAT 
GTCTATATTG GTCAGGTTAT CTATCGTGAA TTGGAACGCC GCGAGAAGGT CAAGATTAAA 
AATGAAGAAG AGTTCAAGGA AAAGAACGGA GATGCTGCCT TTAAAGCAAT GAAAGCATTG 
GGAGAGACAT TTCTGACCAA GATCAACGAG GTGACTCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRNexo ( NM_001300460.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287389.1 Drosophila melanogaster WRN exonuclease (WRNexo), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa22107
Clone ID Related Accession (Same CDS sequence)	NM_001300460.1
Accession Version	NM_001300460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	WRN exonuclease, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAAT ATTTAACAAA AATGCCCATC AAATCAAAAG CAAATGAAGT TCCCAAGGAA 
GCGGGCGTCA AAAAGGAGAC TCCCAAAGTG GCAAGGAAAG CAACAAAAAA GGATACTCCA 
AAAGAGTTGA AAGATAAAGA AAATGCTGGA GATGATAATA CGCCAAAGCA AACGAAAGGA 
AGACCCGGTA GACCGGCTGC CAAGCGAAAA AATCTGGATA CTCCAGATGT GAAGACTGAG 
AAGTTAGCGA TGGAGGAAGA AAATCCGCCC AAGCGCCGGA GTTCCAGGCT GACCAGAAGC 
ACTCGTTCAA TGGCAGAGGA TGGGTCTCCA TCACCGGAGA AGGAAAAGCC CGAAAAGCTT 
CCCTTTATTA AATACAAGGG TGCTATAAAG TATTTCACGG AGAGCCAGGA TATTGCGGCA 
TCTGCTGACG ATGTTTTGCA ATGGGTGGAA AAACAAAAAG ATGAGGTAGT GCCCATGGCC 
TTCGACATGG AGTGGCCCTT CTCTTTCCAA ACAGGACCTG GCAAGTCTGC CGTCATCCAG 
ATATGCGTGG ATGAAAAGTG CTGCTACATA TACCAGCTGA CTAATGTGAA GAAACTGCCT 
GCGGCCCTGG TGGCCCTTAT CAATCATCCC AAAGTGCGAT TGCATGGCGT GAACATAAAG 
AACGATTTCC GAAAGCTGGC ACGTGATTTC CCCGAGGTTA CAGCCGAACC ACTGATAGAG 
AAATGCGTGG ACTTGGGTCT TTGGTGCAAT GAGGTCTGTG AGACCGGCGG GCGCTGGAGC 
TTGGAGCGAT TAACCAACTT TATAGCCAAG AAGGCCATGG ACAAAAGCAA GAAGGTGCGC 
ATGAGCAAGT GGCATGTCAT TCCGCTGGAC GAGAATCAAC TAATGTACGC CGCCATCGAT 
GTCTATATTG GTCAGGTTAT CTATCGTGAA TTGGAACGCC GCGAGAAGGT CAAGATTAAA 
AATGAAGAAG AGTTCAAGGA AAAGAACGGA GATGCTGCCT TTAAAGCAAT GAAAGCATTG 
GGAGAGACAT TTCTGACCAA GATCAACGAG GTGACTCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287389.1
CDS	68..1129
Translation	MEKYLTKMPIKSKANEVPKEAGVKKETPKVARKATKKDTPKELKDKENAGDDNTPKQTKGRPGRPAAKRKNLDTPDVKTEKLAMEEENPPKRRSSRLTRSTRSMAEDGSPSPEKEKPEKLPFIKYKGAIKYFTESQDIAASADDVLQWVEKQKDEVVPMAFDMEWPFSFQTGPGKSAVIQICVDEKCCYIYQLTNVKKLPAALVALINHPKVRLHGVNIKNDFRKLARDFPEVTAEPLIEKCVDLGLWCNEVCETGGRWSLERLTNFIAKKAMDKSKKVRMSKWHVIPLDENQLMYAAIDVYIGQVIYRELERREKVKIKNEEEFKEKNGDAAFKAMKALGETFLTKINEVTL

Target ORF information:

RefSeq Version	NM_001300460.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster WRN exonuclease (WRNexo), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300460.1

ORF Insert Sequence:

ATGGAAAAAT ATTTAACAAA AATGCCCATC AAATCAAAAG CAAATGAAGT TCCCAAGGAA 
GCGGGCGTCA AAAAGGAGAC TCCCAAAGTG GCAAGGAAAG CAACAAAAAA GGATACTCCA 
AAAGAGTTGA AAGATAAAGA AAATGCTGGA GATGATAATA CGCCAAAGCA AACGAAAGGA 
AGACCCGGTA GACCGGCTGC CAAGCGAAAA AATCTGGATA CTCCAGATGT GAAGACTGAG 
AAGTTAGCGA TGGAGGAAGA AAATCCGCCC AAGCGCCGGA GTTCCAGGCT GACCAGAAGC 
ACTCGTTCAA TGGCAGAGGA TGGGTCTCCA TCACCGGAGA AGGAAAAGCC CGAAAAGCTT 
CCCTTTATTA AATACAAGGG TGCTATAAAG TATTTCACGG AGAGCCAGGA TATTGCGGCA 
TCTGCTGACG ATGTTTTGCA ATGGGTGGAA AAACAAAAAG ATGAGGTAGT GCCCATGGCC 
TTCGACATGG AGTGGCCCTT CTCTTTCCAA ACAGGACCTG GCAAGTCTGC CGTCATCCAG 
ATATGCGTGG ATGAAAAGTG CTGCTACATA TACCAGCTGA CTAATGTGAA GAAACTGCCT 
GCGGCCCTGG TGGCCCTTAT CAATCATCCC AAAGTGCGAT TGCATGGCGT GAACATAAAG 
AACGATTTCC GAAAGCTGGC ACGTGATTTC CCCGAGGTTA CAGCCGAACC ACTGATAGAG 
AAATGCGTGG ACTTGGGTCT TTGGTGCAAT GAGGTCTGTG AGACCGGCGG GCGCTGGAGC 
TTGGAGCGAT TAACCAACTT TATAGCCAAG AAGGCCATGG ACAAAAGCAA GAAGGTGCGC 
ATGAGCAAGT GGCATGTCAT TCCGCTGGAC GAGAATCAAC TAATGTACGC CGCCATCGAT 
GTCTATATTG GTCAGGTTAT CTATCGTGAA TTGGAACGCC GCGAGAAGGT CAAGATTAAA 
AATGAAGAAG AGTTCAAGGA AAAGAACGGA GATGCTGCCT TTAAAGCAAT GAAAGCATTG 
GGAGAGACAT TTCTGACCAA GATCAACGAG GTGACTCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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WRNexo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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