Sur-8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sur-8
Entrez Gene ID	42093
Full Name	CG5407 gene product from transcript CG5407-RA
Synonyms	CG5407,CT17146,Dmel\CG5407,SUR-8,Sur8
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 90A5-90A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_142363.7	NP_650620.3	Sur-8, isoform B
NM_169758.4	NP_732231.2	Sur-8, isoform A
NM_001275736.1	NP_001262665.1	Sur-8, isoform E
NM_001275735.1	NP_001262664.1	Sur-8, isoform F

Pathways from BioSystems

Homologies

Homo sapiens (human)	SHOC2	NP_031399.2
Pan troglodytes (chimpanzee)	SHOC2	XP_521602.2
Mus musculus (house mouse)	Shoc2	NP_062632.2
Gallus gallus (chicken)	SHOC2	NP_001026407.1
Danio rerio (zebrafish)	shoc2	NP_001038251.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004485	XP_313783.5
Oryza sativa (rice)	Os10g0119200	NP_001064062.2
Canis lupus familiaris (dog)	SHOC2	XP_535013.2
Bos taurus (cattle)	SHOC2	NP_001095413.1
Drosophila melanogaster (fruit fly)	Sur-8	NP_001262665.1
	SHOC2	XP_001087610.1
Rattus norvegicus (Norway rat)	Shoc2	NP_001013173.1
Caenorhabditis elegans (roundworm)	soc-2	NP_741391.2
Xenopus tropicalis (tropical clawed frog)	shoc2	XP_004915781.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sur-8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sur-8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05220	NM_169758.4 Latest version!	Drosophila melanogaster Sur-8, transcript variant A (Sur-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05220	NM_142363.7 Latest version!	Drosophila melanogaster Sur-8, transcript variant B (Sur-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05220	NM_001275735.1 Latest version!	Drosophila melanogaster Sur-8, transcript variant F (Sur-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa08434	NM_001275736.1 Latest version!	Drosophila melanogaster Sur-8, transcript variant E (Sur-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sur-8 ( NM_142363.7 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650620.3 Drosophila melanogaster Sur-8, transcript variant B (Sur-8), mRNA.

Price & Availability↑

CloneID	OFa05220
Clone ID Related Accession (Same CDS sequence)	NM_142363.7 , NM_169758.4 , NM_001275735.1
Accession Version	NM_142363.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sur-8, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142363.6. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTTGT GTTCCTCGGG CGCAACGGCA TCGACCACAT CGTTATCATC CACGGGCCAG 
GCGGAGCGGA GCGGCGGAGT ACCAGGAGGA GGAGCAGAAG GCGGCGGAGG CAGCGGTAAC 
AGCGGCGGCG GCGGCAAAAC CAGCAACGGA TCCGCCGAGG CCCCAACACT CTGCTTTGCG 
GGAGGATCGG GCACAGCAGG CGCGATCGCC GGAACGGAGG AGCTGTCCAA CGCCAACAGT 
CCGGCCAACG GAGCAGGAGG AGCAACTGGA TCAACCGGCA GTGGACAACA GCCGACTGGC 
AGCAACGGAC ACAGCCACCT TCACAACGAA AACAACGCAA ATATGCCGCC CGAAACGCGT 
CCCAAAATGG TGACCGTCAA GCATCCGGAG TCCAACAAGC CCAAGCCCAC CACAAAGAAA 
AGCAAGCCCA TCCAGGCCGA CCAGGATGTC ATCAAGGCGC TGCAGCGCTG TCGTGATGAA 
GGCATCAAGC GCTTGGACTT GAGCAAATCT TCGATCACGG TGATCCCCAG CACGGTAAAG 
GAGTGCGTCC ATCTCACGGA ACTTTATCTG TACAGCAATA AGATCGGTCA ACTTCCGCCG 
GAAATCGGCT GTCTGGTGAG CCTACGTAAC CTTGCGCTCA ACGAGAACTC GCTGACCTCG 
CTGCCCGAGT CCCTGCAAAA CTGCAGTCAA CTGAAGGTGC TGGATCTGCG GCATAACAAG 
CTGGCGGAGA TTCCGCCCGT GATTTACCGT CTGCGCAGCT TGACCACACT TTACCTGCGC 
TTCAATCGAA TCACCGCCGT GGCAGACGAT CTGCGCCAGC TGGTCAACCT GACCATGTTG 
AGTTTGCGAG AGAACAAGAT CCGGGAGTTG GGCAGCGCCA TCGGCGCGCT GGTGAACCTA 
ACCACGCTGG ACGTTTCGCA CAACCATTTG GAACACCTGC CCGAAGACAT CGGCAACTGT 
GTAAACCTCA GCGCTTTGGA CCTGCAGCAC AACGAACTGC TGGACATACC CGATAGTATT 
GGGAATCTGA AATCCCTGGT GCGGCTGGGC ATGAGATACA ATCGATTAAG TAGCGTGCCG 
GCCACTTTGA AAAACTGCAA GAGCATGGAC GAGTTCAACG TGGAGGGTAA TGGCATAACC 
CAGCTGCCAG ACGGCATGCT GGCCAGCTTA AGCGGATTAA CCACCATCAC TCTATCGAGA 
AATCAGTTTG CAAGTTATCC CACTGGCGGT CCAGCGCAGT TTACCAATGT CTATAGCATT 
AACTTGGAGC ACAACCGGAT CGATAAGATT CCCTATGGGA TCTTCTCGAG GGCCAAGGGT 
CTCACCAAGT TAAACATGAA GGAGAACATG CTGACGGCTT TACCATTAGA CATCGGCACC 
TGGGTGAACA TGGTGGAGCT GAATCTGGCC ACAAATGCGC TACAGAAACT GCCCGATGAT 
ATCATGAACC TGCAAAACCT TGAGATACTC ATCCTGTCAA ACAACATGCT CAAGAAGATT 
CCGAATACAA TTGGAAATTT GCGAAAGCTA AGAATACTGG ACCTGGAGGA GAACCGCATT 
GAGGTGCTGC CGCACGAGAT TGGCCTGCTG CACGAGCTCC AACGTTTGAT CCTGCAAACC 
AATCAAATCA CCATGCTGCC GCGTAGCATC GGCCACTTGG GCAACCTGAC TCACCTGTCT 
GTAAGCGAGA ATAACCTGCA GTTCCTGCCC GAGGAGATTG GTTCGCTAGA GAGCCTTGAG 
AATCTGTACA TCAACCAGAA TCCCGGCTTG GAGAAGCTGC CATTTGAACT GGCTTTGTGT 
CAAAATCTAA AATATCTGAA CATTGACAAG TGCCCGCTGA GCACTATTCC GCCAGAAATC 
CAAGCTGGCG GGCCATCGCT GGTGCTGCAG TGGCTCAAGA TGCACTCACC ATACCGCCAG 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650620.3
CDS	247..2172
Translation	MNLCSSGATASTTSLSSTGQAERSGGVPGGGAEGGGGSGNSGGGGKTSNGSAEAPTLCFAGGSGTAGAIAGTEELSNANSPANGAGGATGSTGSGQQPTGSNGHSHLHNENNANMPPETRPKMVTVKHPESNKPKPTTKKSKPIQADQDVIKALQRCRDEGIKRLDLSKSSITVIPSTVKECVHLTELYLYSNKIGQLPPEIGCLVSLRNLALNENSLTSLPESLQNCSQLKVLDLRHNKLAEIPPVIYRLRSLTTLYLRFNRITAVADDLRQLVNLTMLSLRENKIRELGSAIGALVNLTTLDVSHNHLEHLPEDIGNCVNLSALDLQHNELLDIPDSIGNLKSLVRLGMRYNRLSSVPATLKNCKSMDEFNVEGNGITQLPDGMLASLSGLTTITLSRNQFASYPTGGPAQFTNVYSINLEHNRIDKIPYGIFSRAKGLTKLNMKENMLTALPLDIGTWVNMVELNLATNALQKLPDDIMNLQNLEILILSNNMLKKIPNTIGNLRKLRILDLEENRIEVLPHEIGLLHELQRLILQTNQITMLPRSIGHLGNLTHLSVSENNLQFLPEEIGSLESLENLYINQNPGLEKLPFELALCQNLKYLNIDKCPLSTIPPEIQAGGPSLVLQWLKMHSPYRQM

Target ORF information:

RefSeq Version	NM_142363.7
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sur-8, transcript variant B (Sur-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142363.7

ORF Insert Sequence:

ATGAACTTGT GTTCCTCGGG CGCAACGGCA TCGACCACAT CGTTATCATC CACGGGCCAG 
GCGGAGCGGA GCGGCGGAGT ACCAGGAGGA GGAGCAGAAG GCGGCGGAGG CAGCGGTAAC 
AGCGGCGGCG GCGGCAAAAC CAGCAACGGA TCCGCCGAGG CCCCAACACT CTGCTTTGCG 
GGAGGATCGG GCACAGCAGG CGCGATCGCC GGAACGGAGG AGCTGTCCAA CGCCAACAGT 
CCGGCCAACG GAGCAGGAGG AGCAACTGGA TCAACCGGCA GTGGACAACA GCCGACTGGC 
AGCAACGGAC ACAGCCACCT TCACAACGAA AACAACGCAA ATATGCCGCC CGAAACGCGT 
CCCAAAATGG TGACCGTCAA GCATCCGGAG TCCAACAAGC CCAAGCCCAC CACAAAGAAA 
AGCAAGCCCA TCCAGGCCGA CCAGGATGTC ATCAAGGCGC TGCAGCGCTG TCGTGATGAA 
GGCATCAAGC GCTTGGACTT GAGCAAATCT TCGATCACGG TGATCCCCAG CACGGTAAAG 
GAGTGCGTCC ATCTCACGGA ACTTTATCTG TACAGCAATA AGATCGGTCA ACTTCCGCCG 
GAAATCGGCT GTCTGGTGAG CCTACGTAAC CTTGCGCTCA ACGAGAACTC GCTGACCTCG 
CTGCCCGAGT CCCTGCAAAA CTGCAGTCAA CTGAAGGTGC TGGATCTGCG GCATAACAAG 
CTGGCGGAGA TTCCGCCCGT GATTTACCGT CTGCGCAGCT TGACCACACT TTACCTGCGC 
TTCAATCGAA TCACCGCCGT GGCAGACGAT CTGCGCCAGC TGGTCAACCT GACCATGTTG 
AGTTTGCGAG AGAACAAGAT CCGGGAGTTG GGCAGCGCCA TCGGCGCGCT GGTGAACCTA 
ACCACGCTGG ACGTTTCGCA CAACCATTTG GAACACCTGC CCGAAGACAT CGGCAACTGT 
GTAAACCTCA GCGCTTTGGA CCTGCAGCAC AACGAACTGC TGGACATACC CGATAGTATT 
GGGAATCTGA AATCCCTGGT GCGGCTGGGC ATGAGATACA ATCGATTAAG TAGCGTGCCG 
GCCACTTTGA AAAACTGCAA GAGCATGGAC GAGTTCAACG TGGAGGGTAA TGGCATAACC 
CAGCTGCCAG ACGGCATGCT GGCCAGCTTA AGCGGATTAA CCACCATCAC TCTATCGAGA 
AATCAGTTTG CAAGTTATCC CACTGGCGGT CCAGCGCAGT TTACCAATGT CTATAGCATT 
AACTTGGAGC ACAACCGGAT CGATAAGATT CCCTATGGGA TCTTCTCGAG GGCCAAGGGT 
CTCACCAAGT TAAACATGAA GGAGAACATG CTGACGGCTT TACCATTAGA CATCGGCACC 
TGGGTGAACA TGGTGGAGCT GAATCTGGCC ACAAATGCGC TACAGAAACT GCCCGATGAT 
ATCATGAACC TGCAAAACCT TGAGATACTC ATCCTGTCAA ACAACATGCT CAAGAAGATT 
CCGAATACAA TTGGAAATTT GCGAAAGCTA AGAATACTGG ACCTGGAGGA GAACCGCATT 
GAGGTGCTGC CGCACGAGAT TGGCCTGCTG CACGAGCTCC AACGTTTGAT CCTGCAAACC 
AATCAAATCA CCATGCTGCC GCGTAGCATC GGCCACTTGG GCAACCTGAC TCACCTGTCT 
GTAAGCGAGA ATAACCTGCA GTTCCTGCCC GAGGAGATTG GTTCGCTAGA GAGCCTTGAG 
AATCTGTACA TCAACCAGAA TCCCGGCTTG GAGAAGCTGC CATTTGAACT GGCTTTGTGT 
CAAAATCTAA AATATCTGAA CATTGACAAG TGCCCGCTGA GCACTATTCC GCCAGAAATC 
CAAGCTGGCG GGCCATCGCT GGTGCTGCAG TGGCTCAAGA TGCACTCACC ATACCGCCAG 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sur-8 ( NM_169758.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732231.2 Drosophila melanogaster Sur-8, transcript variant A (Sur-8), mRNA.

Price & Availability↑

CloneID	OFa05220
Clone ID Related Accession (Same CDS sequence)	NM_142363.7 , NM_169758.4 , NM_001275735.1
Accession Version	NM_169758.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sur-8, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169758.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTTGT GTTCCTCGGG CGCAACGGCA TCGACCACAT CGTTATCATC CACGGGCCAG 
GCGGAGCGGA GCGGCGGAGT ACCAGGAGGA GGAGCAGAAG GCGGCGGAGG CAGCGGTAAC 
AGCGGCGGCG GCGGCAAAAC CAGCAACGGA TCCGCCGAGG CCCCAACACT CTGCTTTGCG 
GGAGGATCGG GCACAGCAGG CGCGATCGCC GGAACGGAGG AGCTGTCCAA CGCCAACAGT 
CCGGCCAACG GAGCAGGAGG AGCAACTGGA TCAACCGGCA GTGGACAACA GCCGACTGGC 
AGCAACGGAC ACAGCCACCT TCACAACGAA AACAACGCAA ATATGCCGCC CGAAACGCGT 
CCCAAAATGG TGACCGTCAA GCATCCGGAG TCCAACAAGC CCAAGCCCAC CACAAAGAAA 
AGCAAGCCCA TCCAGGCCGA CCAGGATGTC ATCAAGGCGC TGCAGCGCTG TCGTGATGAA 
GGCATCAAGC GCTTGGACTT GAGCAAATCT TCGATCACGG TGATCCCCAG CACGGTAAAG 
GAGTGCGTCC ATCTCACGGA ACTTTATCTG TACAGCAATA AGATCGGTCA ACTTCCGCCG 
GAAATCGGCT GTCTGGTGAG CCTACGTAAC CTTGCGCTCA ACGAGAACTC GCTGACCTCG 
CTGCCCGAGT CCCTGCAAAA CTGCAGTCAA CTGAAGGTGC TGGATCTGCG GCATAACAAG 
CTGGCGGAGA TTCCGCCCGT GATTTACCGT CTGCGCAGCT TGACCACACT TTACCTGCGC 
TTCAATCGAA TCACCGCCGT GGCAGACGAT CTGCGCCAGC TGGTCAACCT GACCATGTTG 
AGTTTGCGAG AGAACAAGAT CCGGGAGTTG GGCAGCGCCA TCGGCGCGCT GGTGAACCTA 
ACCACGCTGG ACGTTTCGCA CAACCATTTG GAACACCTGC CCGAAGACAT CGGCAACTGT 
GTAAACCTCA GCGCTTTGGA CCTGCAGCAC AACGAACTGC TGGACATACC CGATAGTATT 
GGGAATCTGA AATCCCTGGT GCGGCTGGGC ATGAGATACA ATCGATTAAG TAGCGTGCCG 
GCCACTTTGA AAAACTGCAA GAGCATGGAC GAGTTCAACG TGGAGGGTAA TGGCATAACC 
CAGCTGCCAG ACGGCATGCT GGCCAGCTTA AGCGGATTAA CCACCATCAC TCTATCGAGA 
AATCAGTTTG CAAGTTATCC CACTGGCGGT CCAGCGCAGT TTACCAATGT CTATAGCATT 
AACTTGGAGC ACAACCGGAT CGATAAGATT CCCTATGGGA TCTTCTCGAG GGCCAAGGGT 
CTCACCAAGT TAAACATGAA GGAGAACATG CTGACGGCTT TACCATTAGA CATCGGCACC 
TGGGTGAACA TGGTGGAGCT GAATCTGGCC ACAAATGCGC TACAGAAACT GCCCGATGAT 
ATCATGAACC TGCAAAACCT TGAGATACTC ATCCTGTCAA ACAACATGCT CAAGAAGATT 
CCGAATACAA TTGGAAATTT GCGAAAGCTA AGAATACTGG ACCTGGAGGA GAACCGCATT 
GAGGTGCTGC CGCACGAGAT TGGCCTGCTG CACGAGCTCC AACGTTTGAT CCTGCAAACC 
AATCAAATCA CCATGCTGCC GCGTAGCATC GGCCACTTGG GCAACCTGAC TCACCTGTCT 
GTAAGCGAGA ATAACCTGCA GTTCCTGCCC GAGGAGATTG GTTCGCTAGA GAGCCTTGAG 
AATCTGTACA TCAACCAGAA TCCCGGCTTG GAGAAGCTGC CATTTGAACT GGCTTTGTGT 
CAAAATCTAA AATATCTGAA CATTGACAAG TGCCCGCTGA GCACTATTCC GCCAGAAATC 
CAAGCTGGCG GGCCATCGCT GGTGCTGCAG TGGCTCAAGA TGCACTCACC ATACCGCCAG 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732231.2
CDS	475..2400
Translation	MNLCSSGATASTTSLSSTGQAERSGGVPGGGAEGGGGSGNSGGGGKTSNGSAEAPTLCFAGGSGTAGAIAGTEELSNANSPANGAGGATGSTGSGQQPTGSNGHSHLHNENNANMPPETRPKMVTVKHPESNKPKPTTKKSKPIQADQDVIKALQRCRDEGIKRLDLSKSSITVIPSTVKECVHLTELYLYSNKIGQLPPEIGCLVSLRNLALNENSLTSLPESLQNCSQLKVLDLRHNKLAEIPPVIYRLRSLTTLYLRFNRITAVADDLRQLVNLTMLSLRENKIRELGSAIGALVNLTTLDVSHNHLEHLPEDIGNCVNLSALDLQHNELLDIPDSIGNLKSLVRLGMRYNRLSSVPATLKNCKSMDEFNVEGNGITQLPDGMLASLSGLTTITLSRNQFASYPTGGPAQFTNVYSINLEHNRIDKIPYGIFSRAKGLTKLNMKENMLTALPLDIGTWVNMVELNLATNALQKLPDDIMNLQNLEILILSNNMLKKIPNTIGNLRKLRILDLEENRIEVLPHEIGLLHELQRLILQTNQITMLPRSIGHLGNLTHLSVSENNLQFLPEEIGSLESLENLYINQNPGLEKLPFELALCQNLKYLNIDKCPLSTIPPEIQAGGPSLVLQWLKMHSPYRQM

Target ORF information:

RefSeq Version	NM_169758.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sur-8, transcript variant A (Sur-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169758.4

ORF Insert Sequence:

ATGAACTTGT GTTCCTCGGG CGCAACGGCA TCGACCACAT CGTTATCATC CACGGGCCAG 
GCGGAGCGGA GCGGCGGAGT ACCAGGAGGA GGAGCAGAAG GCGGCGGAGG CAGCGGTAAC 
AGCGGCGGCG GCGGCAAAAC CAGCAACGGA TCCGCCGAGG CCCCAACACT CTGCTTTGCG 
GGAGGATCGG GCACAGCAGG CGCGATCGCC GGAACGGAGG AGCTGTCCAA CGCCAACAGT 
CCGGCCAACG GAGCAGGAGG AGCAACTGGA TCAACCGGCA GTGGACAACA GCCGACTGGC 
AGCAACGGAC ACAGCCACCT TCACAACGAA AACAACGCAA ATATGCCGCC CGAAACGCGT 
CCCAAAATGG TGACCGTCAA GCATCCGGAG TCCAACAAGC CCAAGCCCAC CACAAAGAAA 
AGCAAGCCCA TCCAGGCCGA CCAGGATGTC ATCAAGGCGC TGCAGCGCTG TCGTGATGAA 
GGCATCAAGC GCTTGGACTT GAGCAAATCT TCGATCACGG TGATCCCCAG CACGGTAAAG 
GAGTGCGTCC ATCTCACGGA ACTTTATCTG TACAGCAATA AGATCGGTCA ACTTCCGCCG 
GAAATCGGCT GTCTGGTGAG CCTACGTAAC CTTGCGCTCA ACGAGAACTC GCTGACCTCG 
CTGCCCGAGT CCCTGCAAAA CTGCAGTCAA CTGAAGGTGC TGGATCTGCG GCATAACAAG 
CTGGCGGAGA TTCCGCCCGT GATTTACCGT CTGCGCAGCT TGACCACACT TTACCTGCGC 
TTCAATCGAA TCACCGCCGT GGCAGACGAT CTGCGCCAGC TGGTCAACCT GACCATGTTG 
AGTTTGCGAG AGAACAAGAT CCGGGAGTTG GGCAGCGCCA TCGGCGCGCT GGTGAACCTA 
ACCACGCTGG ACGTTTCGCA CAACCATTTG GAACACCTGC CCGAAGACAT CGGCAACTGT 
GTAAACCTCA GCGCTTTGGA CCTGCAGCAC AACGAACTGC TGGACATACC CGATAGTATT 
GGGAATCTGA AATCCCTGGT GCGGCTGGGC ATGAGATACA ATCGATTAAG TAGCGTGCCG 
GCCACTTTGA AAAACTGCAA GAGCATGGAC GAGTTCAACG TGGAGGGTAA TGGCATAACC 
CAGCTGCCAG ACGGCATGCT GGCCAGCTTA AGCGGATTAA CCACCATCAC TCTATCGAGA 
AATCAGTTTG CAAGTTATCC CACTGGCGGT CCAGCGCAGT TTACCAATGT CTATAGCATT 
AACTTGGAGC ACAACCGGAT CGATAAGATT CCCTATGGGA TCTTCTCGAG GGCCAAGGGT 
CTCACCAAGT TAAACATGAA GGAGAACATG CTGACGGCTT TACCATTAGA CATCGGCACC 
TGGGTGAACA TGGTGGAGCT GAATCTGGCC ACAAATGCGC TACAGAAACT GCCCGATGAT 
ATCATGAACC TGCAAAACCT TGAGATACTC ATCCTGTCAA ACAACATGCT CAAGAAGATT 
CCGAATACAA TTGGAAATTT GCGAAAGCTA AGAATACTGG ACCTGGAGGA GAACCGCATT 
GAGGTGCTGC CGCACGAGAT TGGCCTGCTG CACGAGCTCC AACGTTTGAT CCTGCAAACC 
AATCAAATCA CCATGCTGCC GCGTAGCATC GGCCACTTGG GCAACCTGAC TCACCTGTCT 
GTAAGCGAGA ATAACCTGCA GTTCCTGCCC GAGGAGATTG GTTCGCTAGA GAGCCTTGAG 
AATCTGTACA TCAACCAGAA TCCCGGCTTG GAGAAGCTGC CATTTGAACT GGCTTTGTGT 
CAAAATCTAA AATATCTGAA CATTGACAAG TGCCCGCTGA GCACTATTCC GCCAGAAATC 
CAAGCTGGCG GGCCATCGCT GGTGCTGCAG TGGCTCAAGA TGCACTCACC ATACCGCCAG 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sur-8 ( NM_001275735.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262664.1 Drosophila melanogaster Sur-8, transcript variant F (Sur-8), mRNA.

Price & Availability↑

CloneID	OFa05220
Clone ID Related Accession (Same CDS sequence)	NM_142363.7 , NM_169758.4 , NM_001275735.1
Accession Version	NM_001275735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sur-8, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTTGT GTTCCTCGGG CGCAACGGCA TCGACCACAT CGTTATCATC CACGGGCCAG 
GCGGAGCGGA GCGGCGGAGT ACCAGGAGGA GGAGCAGAAG GCGGCGGAGG CAGCGGTAAC 
AGCGGCGGCG GCGGCAAAAC CAGCAACGGA TCCGCCGAGG CCCCAACACT CTGCTTTGCG 
GGAGGATCGG GCACAGCAGG CGCGATCGCC GGAACGGAGG AGCTGTCCAA CGCCAACAGT 
CCGGCCAACG GAGCAGGAGG AGCAACTGGA TCAACCGGCA GTGGACAACA GCCGACTGGC 
AGCAACGGAC ACAGCCACCT TCACAACGAA AACAACGCAA ATATGCCGCC CGAAACGCGT 
CCCAAAATGG TGACCGTCAA GCATCCGGAG TCCAACAAGC CCAAGCCCAC CACAAAGAAA 
AGCAAGCCCA TCCAGGCCGA CCAGGATGTC ATCAAGGCGC TGCAGCGCTG TCGTGATGAA 
GGCATCAAGC GCTTGGACTT GAGCAAATCT TCGATCACGG TGATCCCCAG CACGGTAAAG 
GAGTGCGTCC ATCTCACGGA ACTTTATCTG TACAGCAATA AGATCGGTCA ACTTCCGCCG 
GAAATCGGCT GTCTGGTGAG CCTACGTAAC CTTGCGCTCA ACGAGAACTC GCTGACCTCG 
CTGCCCGAGT CCCTGCAAAA CTGCAGTCAA CTGAAGGTGC TGGATCTGCG GCATAACAAG 
CTGGCGGAGA TTCCGCCCGT GATTTACCGT CTGCGCAGCT TGACCACACT TTACCTGCGC 
TTCAATCGAA TCACCGCCGT GGCAGACGAT CTGCGCCAGC TGGTCAACCT GACCATGTTG 
AGTTTGCGAG AGAACAAGAT CCGGGAGTTG GGCAGCGCCA TCGGCGCGCT GGTGAACCTA 
ACCACGCTGG ACGTTTCGCA CAACCATTTG GAACACCTGC CCGAAGACAT CGGCAACTGT 
GTAAACCTCA GCGCTTTGGA CCTGCAGCAC AACGAACTGC TGGACATACC CGATAGTATT 
GGGAATCTGA AATCCCTGGT GCGGCTGGGC ATGAGATACA ATCGATTAAG TAGCGTGCCG 
GCCACTTTGA AAAACTGCAA GAGCATGGAC GAGTTCAACG TGGAGGGTAA TGGCATAACC 
CAGCTGCCAG ACGGCATGCT GGCCAGCTTA AGCGGATTAA CCACCATCAC TCTATCGAGA 
AATCAGTTTG CAAGTTATCC CACTGGCGGT CCAGCGCAGT TTACCAATGT CTATAGCATT 
AACTTGGAGC ACAACCGGAT CGATAAGATT CCCTATGGGA TCTTCTCGAG GGCCAAGGGT 
CTCACCAAGT TAAACATGAA GGAGAACATG CTGACGGCTT TACCATTAGA CATCGGCACC 
TGGGTGAACA TGGTGGAGCT GAATCTGGCC ACAAATGCGC TACAGAAACT GCCCGATGAT 
ATCATGAACC TGCAAAACCT TGAGATACTC ATCCTGTCAA ACAACATGCT CAAGAAGATT 
CCGAATACAA TTGGAAATTT GCGAAAGCTA AGAATACTGG ACCTGGAGGA GAACCGCATT 
GAGGTGCTGC CGCACGAGAT TGGCCTGCTG CACGAGCTCC AACGTTTGAT CCTGCAAACC 
AATCAAATCA CCATGCTGCC GCGTAGCATC GGCCACTTGG GCAACCTGAC TCACCTGTCT 
GTAAGCGAGA ATAACCTGCA GTTCCTGCCC GAGGAGATTG GTTCGCTAGA GAGCCTTGAG 
AATCTGTACA TCAACCAGAA TCCCGGCTTG GAGAAGCTGC CATTTGAACT GGCTTTGTGT 
CAAAATCTAA AATATCTGAA CATTGACAAG TGCCCGCTGA GCACTATTCC GCCAGAAATC 
CAAGCTGGCG GGCCATCGCT GGTGCTGCAG TGGCTCAAGA TGCACTCACC ATACCGCCAG 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262664.1
CDS	247..2172
Translation	MNLCSSGATASTTSLSSTGQAERSGGVPGGGAEGGGGSGNSGGGGKTSNGSAEAPTLCFAGGSGTAGAIAGTEELSNANSPANGAGGATGSTGSGQQPTGSNGHSHLHNENNANMPPETRPKMVTVKHPESNKPKPTTKKSKPIQADQDVIKALQRCRDEGIKRLDLSKSSITVIPSTVKECVHLTELYLYSNKIGQLPPEIGCLVSLRNLALNENSLTSLPESLQNCSQLKVLDLRHNKLAEIPPVIYRLRSLTTLYLRFNRITAVADDLRQLVNLTMLSLRENKIRELGSAIGALVNLTTLDVSHNHLEHLPEDIGNCVNLSALDLQHNELLDIPDSIGNLKSLVRLGMRYNRLSSVPATLKNCKSMDEFNVEGNGITQLPDGMLASLSGLTTITLSRNQFASYPTGGPAQFTNVYSINLEHNRIDKIPYGIFSRAKGLTKLNMKENMLTALPLDIGTWVNMVELNLATNALQKLPDDIMNLQNLEILILSNNMLKKIPNTIGNLRKLRILDLEENRIEVLPHEIGLLHELQRLILQTNQITMLPRSIGHLGNLTHLSVSENNLQFLPEEIGSLESLENLYINQNPGLEKLPFELALCQNLKYLNIDKCPLSTIPPEIQAGGPSLVLQWLKMHSPYRQM

Target ORF information:

RefSeq Version	NM_001275735.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sur-8, transcript variant F (Sur-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275735.1

ORF Insert Sequence:

ATGAACTTGT GTTCCTCGGG CGCAACGGCA TCGACCACAT CGTTATCATC CACGGGCCAG 
GCGGAGCGGA GCGGCGGAGT ACCAGGAGGA GGAGCAGAAG GCGGCGGAGG CAGCGGTAAC 
AGCGGCGGCG GCGGCAAAAC CAGCAACGGA TCCGCCGAGG CCCCAACACT CTGCTTTGCG 
GGAGGATCGG GCACAGCAGG CGCGATCGCC GGAACGGAGG AGCTGTCCAA CGCCAACAGT 
CCGGCCAACG GAGCAGGAGG AGCAACTGGA TCAACCGGCA GTGGACAACA GCCGACTGGC 
AGCAACGGAC ACAGCCACCT TCACAACGAA AACAACGCAA ATATGCCGCC CGAAACGCGT 
CCCAAAATGG TGACCGTCAA GCATCCGGAG TCCAACAAGC CCAAGCCCAC CACAAAGAAA 
AGCAAGCCCA TCCAGGCCGA CCAGGATGTC ATCAAGGCGC TGCAGCGCTG TCGTGATGAA 
GGCATCAAGC GCTTGGACTT GAGCAAATCT TCGATCACGG TGATCCCCAG CACGGTAAAG 
GAGTGCGTCC ATCTCACGGA ACTTTATCTG TACAGCAATA AGATCGGTCA ACTTCCGCCG 
GAAATCGGCT GTCTGGTGAG CCTACGTAAC CTTGCGCTCA ACGAGAACTC GCTGACCTCG 
CTGCCCGAGT CCCTGCAAAA CTGCAGTCAA CTGAAGGTGC TGGATCTGCG GCATAACAAG 
CTGGCGGAGA TTCCGCCCGT GATTTACCGT CTGCGCAGCT TGACCACACT TTACCTGCGC 
TTCAATCGAA TCACCGCCGT GGCAGACGAT CTGCGCCAGC TGGTCAACCT GACCATGTTG 
AGTTTGCGAG AGAACAAGAT CCGGGAGTTG GGCAGCGCCA TCGGCGCGCT GGTGAACCTA 
ACCACGCTGG ACGTTTCGCA CAACCATTTG GAACACCTGC CCGAAGACAT CGGCAACTGT 
GTAAACCTCA GCGCTTTGGA CCTGCAGCAC AACGAACTGC TGGACATACC CGATAGTATT 
GGGAATCTGA AATCCCTGGT GCGGCTGGGC ATGAGATACA ATCGATTAAG TAGCGTGCCG 
GCCACTTTGA AAAACTGCAA GAGCATGGAC GAGTTCAACG TGGAGGGTAA TGGCATAACC 
CAGCTGCCAG ACGGCATGCT GGCCAGCTTA AGCGGATTAA CCACCATCAC TCTATCGAGA 
AATCAGTTTG CAAGTTATCC CACTGGCGGT CCAGCGCAGT TTACCAATGT CTATAGCATT 
AACTTGGAGC ACAACCGGAT CGATAAGATT CCCTATGGGA TCTTCTCGAG GGCCAAGGGT 
CTCACCAAGT TAAACATGAA GGAGAACATG CTGACGGCTT TACCATTAGA CATCGGCACC 
TGGGTGAACA TGGTGGAGCT GAATCTGGCC ACAAATGCGC TACAGAAACT GCCCGATGAT 
ATCATGAACC TGCAAAACCT TGAGATACTC ATCCTGTCAA ACAACATGCT CAAGAAGATT 
CCGAATACAA TTGGAAATTT GCGAAAGCTA AGAATACTGG ACCTGGAGGA GAACCGCATT 
GAGGTGCTGC CGCACGAGAT TGGCCTGCTG CACGAGCTCC AACGTTTGAT CCTGCAAACC 
AATCAAATCA CCATGCTGCC GCGTAGCATC GGCCACTTGG GCAACCTGAC TCACCTGTCT 
GTAAGCGAGA ATAACCTGCA GTTCCTGCCC GAGGAGATTG GTTCGCTAGA GAGCCTTGAG 
AATCTGTACA TCAACCAGAA TCCCGGCTTG GAGAAGCTGC CATTTGAACT GGCTTTGTGT 
CAAAATCTAA AATATCTGAA CATTGACAAG TGCCCGCTGA GCACTATTCC GCCAGAAATC 
CAAGCTGGCG GGCCATCGCT GGTGCTGCAG TGGCTCAAGA TGCACTCACC ATACCGCCAG 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sur-8 ( NM_001275736.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262665.1 Drosophila melanogaster Sur-8, transcript variant E (Sur-8), mRNA.

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CloneID	OFa08434
Clone ID Related Accession (Same CDS sequence)	NM_001275736.1
Accession Version	NM_001275736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sur-8, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTTGT GTTCCTCGGG CGCAACGGCA TCGACCACAT CGTTATCATC CACGGGCCAG 
GCGGAGCGGA GCGGCGGAGT ACCAGGAGGA GGAGCAGAAG GCGGCGGAGG CAGCGGTAAC 
AGCGGCGGCG GCGGCAAAAC CAGCAACGGA TCCGCCGAGG CCCCAACACT CTGCTTTGCG 
GGAGGATCGG GCACAGCAGG CGCGATCGCC GGAACGGAGG AGCTGTCCAA CGCCAACAGT 
CCGGCCAACG GAGCAGGAGG AGCAACTGGA TCAACCGGCA GTGGACAACA GCCGACTGGC 
AGCAACGGAC ACAGCCACCT TCACAACGAA AACAACGCAA ATATGCCGCC CGAAACGCGT 
CCCAAAATGG TGACCGTCAA GCATCCGGAG TCCAACAAGC CCAAGCCCAC CACAAAGAAA 
AGCAAGCCCA TCCAGGCCGA CCAGGATGTC ATCAAGGCGC TGCAGCGCTG TCGTGATGAA 
GGCATCAAGC GCTTGGACTT GAGCAAATCT TCGATCACGG TGATCCCCAG CACGGTAAAG 
GAGTGCGTCC ATCTCACGGA ACTTTATCTG TACAGCAATA AGATCGGTCA ACTTCCGCCG 
GAAATCGGCT GTCTGGTGAG CCTACGTAAC CTTGCGCTCA ACGAGAACTC GCTGACCTCG 
CTGCCCGAGT CCCTGCAAAA CTGCAGTCAA CTGAAGGTGC TGGATCTGCG GCATAACAAG 
CTGGCGGAGA TTCCGCCCGT GATTTACCGT CTGCGCAGCT TGACCACACT TTACCTGCGC 
TTCAATCGAA TCACCGCCGT GGCAGACGAT CTGCGCCAGC TGGTCAACCT GACCATGTTG 
AGTTTGCGAG AGAACAAGAT CCGGGAGTTG GGCAGCGCCA TCGGCGCGCT GGTGAACCTA 
ACCACGCTGG ACGTTTCGCA CAACCATTTG GAACACCTGC CCGAAGACAT CGGCAACTGT 
GTAAACCTCA GCGCTTTGGA CCTGCAGCAC AACGAACTGC TGGACATACC CGATAGTATT 
GGGAATCTGA AATCCCTGGT GCGGCTGGGC ATGAGATACA ATCGATTAAG TAGCGTGCCG 
GCCACTTTGA AAAACTGCAA GAGCATGGAC GAGTTCAACG TGGAGGGTAA TGGCATAACC 
CAGCTGCCAG ACGGCATGCT GGCCAGCTTA AGCGGATTAA CCACCATCAC TCTATCGAGA 
AATCAGTTTG CAAGTTATCC CACTGGCGGT CCAGCGCAGT TTACCAATGT CTATAGCATT 
AACTTGGAGC ACAACCGGAT CGATAAGATT CCCTATGGGA TCTTCTCGAG GGCCAAGGGT 
CTCACCAAGT TAAACATGAA GGAGAACATG CTGACGGCTT TACCATTAGA CATCGGCACC 
TGGGTGAACA TGGTGGAGCT GAATCTGGCC ACAAATGCGC TACAGAAACT GCCCGATGAT 
ATCATGAACC TGCAAAACCT TGAGATACTC ATCCTGTCAA ACAACATGCT CAAGAAGATT 
CCGAATACAA TTGGAAATTT GCGAAAGCTA AGAATACTGG ACCTGGAGGA GAACCGCATT 
GAGGTGCTGC CGCACGAGAT TGGCCTGCTG CACGAGCTCC AACGTTTGAT CCTGCAAACC 
AATCAAATCA CCATGCTGCC GCGTAGCATC GGCCACTTGG GCAACCTGAC TCACCTGTCT 
GTAAGCGAGA ATAACCTGCA GTTCCTGCCC GAGGAGATTG GTTCGCTAGA GAGCCTTGAG 
AATCTGTACA TCAACCAGAA TCCCGGCTTG GAGAAGCTGC CATTTGAACT GGCTTTGTGT 
CAAAATCTAA AATATCTGAA CATTGACAAG TGCCCGCTGA GCACTATTCC GCCAGAAATC 
CAAGCTGGCG GGCCATCGCT GGTGCTGCAG TGGCTCAAGA TGCACTCACC ATACCGCCAG 
ATGTGAGATG TGGACGGCGT TTGGCGCACC GTCTACGTGG GCAGCGACTG CTACTATCAG 
TACGAGCTGC AGACGGTGAA CCAGGCGCCC GGTGCTGGTG GCAACGGTGG TGGAGGAGCA 
GCGGCAGCAG GAGGATCCGC ATCCAGAAGC AGCGACCGTC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262665.1
CDS	475..2559
Translation	MNLCSSGATASTTSLSSTGQAERSGGVPGGGAEGGGGSGNSGGGGKTSNGSAEAPTLCFAGGSGTAGAIAGTEELSNANSPANGAGGATGSTGSGQQPTGSNGHSHLHNENNANMPPETRPKMVTVKHPESNKPKPTTKKSKPIQADQDVIKALQRCRDEGIKRLDLSKSSITVIPSTVKECVHLTELYLYSNKIGQLPPEIGCLVSLRNLALNENSLTSLPESLQNCSQLKVLDLRHNKLAEIPPVIYRLRSLTTLYLRFNRITAVADDLRQLVNLTMLSLRENKIRELGSAIGALVNLTTLDVSHNHLEHLPEDIGNCVNLSALDLQHNELLDIPDSIGNLKSLVRLGMRYNRLSSVPATLKNCKSMDEFNVEGNGITQLPDGMLASLSGLTTITLSRNQFASYPTGGPAQFTNVYSINLEHNRIDKIPYGIFSRAKGLTKLNMKENMLTALPLDIGTWVNMVELNLATNALQKLPDDIMNLQNLEILILSNNMLKKIPNTIGNLRKLRILDLEENRIEVLPHEIGLLHELQRLILQTNQITMLPRSIGHLGNLTHLSVSENNLQFLPEEIGSLESLENLYINQNPGLEKLPFELALCQNLKYLNIDKCPLSTIPPEIQAGGPSLVLQWLKMHSPYRQM*DVDGVWRTVYVGSDCYYQYELQTVNQAPGAGGNGGGGAAAAGGSASRSSDRR

Target ORF information:

RefSeq Version	NM_001275736.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sur-8, transcript variant E (Sur-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275736.1

ORF Insert Sequence:

ATGAACTTGT GTTCCTCGGG CGCAACGGCA TCGACCACAT CGTTATCATC CACGGGCCAG 
GCGGAGCGGA GCGGCGGAGT ACCAGGAGGA GGAGCAGAAG GCGGCGGAGG CAGCGGTAAC 
AGCGGCGGCG GCGGCAAAAC CAGCAACGGA TCCGCCGAGG CCCCAACACT CTGCTTTGCG 
GGAGGATCGG GCACAGCAGG CGCGATCGCC GGAACGGAGG AGCTGTCCAA CGCCAACAGT 
CCGGCCAACG GAGCAGGAGG AGCAACTGGA TCAACCGGCA GTGGACAACA GCCGACTGGC 
AGCAACGGAC ACAGCCACCT TCACAACGAA AACAACGCAA ATATGCCGCC CGAAACGCGT 
CCCAAAATGG TGACCGTCAA GCATCCGGAG TCCAACAAGC CCAAGCCCAC CACAAAGAAA 
AGCAAGCCCA TCCAGGCCGA CCAGGATGTC ATCAAGGCGC TGCAGCGCTG TCGTGATGAA 
GGCATCAAGC GCTTGGACTT GAGCAAATCT TCGATCACGG TGATCCCCAG CACGGTAAAG 
GAGTGCGTCC ATCTCACGGA ACTTTATCTG TACAGCAATA AGATCGGTCA ACTTCCGCCG 
GAAATCGGCT GTCTGGTGAG CCTACGTAAC CTTGCGCTCA ACGAGAACTC GCTGACCTCG 
CTGCCCGAGT CCCTGCAAAA CTGCAGTCAA CTGAAGGTGC TGGATCTGCG GCATAACAAG 
CTGGCGGAGA TTCCGCCCGT GATTTACCGT CTGCGCAGCT TGACCACACT TTACCTGCGC 
TTCAATCGAA TCACCGCCGT GGCAGACGAT CTGCGCCAGC TGGTCAACCT GACCATGTTG 
AGTTTGCGAG AGAACAAGAT CCGGGAGTTG GGCAGCGCCA TCGGCGCGCT GGTGAACCTA 
ACCACGCTGG ACGTTTCGCA CAACCATTTG GAACACCTGC CCGAAGACAT CGGCAACTGT 
GTAAACCTCA GCGCTTTGGA CCTGCAGCAC AACGAACTGC TGGACATACC CGATAGTATT 
GGGAATCTGA AATCCCTGGT GCGGCTGGGC ATGAGATACA ATCGATTAAG TAGCGTGCCG 
GCCACTTTGA AAAACTGCAA GAGCATGGAC GAGTTCAACG TGGAGGGTAA TGGCATAACC 
CAGCTGCCAG ACGGCATGCT GGCCAGCTTA AGCGGATTAA CCACCATCAC TCTATCGAGA 
AATCAGTTTG CAAGTTATCC CACTGGCGGT CCAGCGCAGT TTACCAATGT CTATAGCATT 
AACTTGGAGC ACAACCGGAT CGATAAGATT CCCTATGGGA TCTTCTCGAG GGCCAAGGGT 
CTCACCAAGT TAAACATGAA GGAGAACATG CTGACGGCTT TACCATTAGA CATCGGCACC 
TGGGTGAACA TGGTGGAGCT GAATCTGGCC ACAAATGCGC TACAGAAACT GCCCGATGAT 
ATCATGAACC TGCAAAACCT TGAGATACTC ATCCTGTCAA ACAACATGCT CAAGAAGATT 
CCGAATACAA TTGGAAATTT GCGAAAGCTA AGAATACTGG ACCTGGAGGA GAACCGCATT 
GAGGTGCTGC CGCACGAGAT TGGCCTGCTG CACGAGCTCC AACGTTTGAT CCTGCAAACC 
AATCAAATCA CCATGCTGCC GCGTAGCATC GGCCACTTGG GCAACCTGAC TCACCTGTCT 
GTAAGCGAGA ATAACCTGCA GTTCCTGCCC GAGGAGATTG GTTCGCTAGA GAGCCTTGAG 
AATCTGTACA TCAACCAGAA TCCCGGCTTG GAGAAGCTGC CATTTGAACT GGCTTTGTGT 
CAAAATCTAA AATATCTGAA CATTGACAAG TGCCCGCTGA GCACTATTCC GCCAGAAATC 
CAAGCTGGCG GGCCATCGCT GGTGCTGCAG TGGCTCAAGA TGCACTCACC ATACCGCCAG 
ATGTGAGATG TGGACGGCGT TTGGCGCACC GTCTACGTGG GCAGCGACTG CTACTATCAG 
TACGAGCTGC AGACGGTGAA CCAGGCGCCC GGTGCTGGTG GCAACGGTGG TGGAGGAGCA 
GCGGCAGCAG GAGGATCCGC ATCCAGAAGC AGCGACCGTC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sur-8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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