GckIII cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	GckIII
Entrez Gene ID	42064
Full Name	Germinal centre kinase III
Synonyms	CG5169,Dmel\CG5169,GCKIII,Gck III,STLK3,dGCKIII,wheezy
General protein information	Preferred Names Germinal centre kinase III Names CG5169 gene product from transcript CG5169-RA CG5169-PA GckIII-PA germinal center kinase III germinal centre kinase III wheezy
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89E12-89E12

mRNA and Protein(s)

mRNA	Protein	Name
NM_142339.3	NP_650596.1	germinal centre kinase III

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	mst4	NP_001164989.1
Pan troglodytes (chimpanzee)	MST4	XP_003317731.1
Bos taurus (cattle)	MST4	NP_001157258.1
Canis lupus familiaris (dog)	MST4	XP_549261.3
Rattus norvegicus (Norway rat)	Mst4	NP_001178665.1
Homo sapiens (human)	MST4	NP_057626.2
Mus musculus (house mouse)	2610018G03Rik	NP_598490.1
Danio rerio (zebrafish)	mst4	NP_001120949.1
Drosophila melanogaster (fruit fly)	GckIII	NP_650596.1
	MST4	XP_002806444.1
Gallus gallus (chicken)	MST4	NP_001026288.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CCM3 and GCKIII prevent tube dilation in Drosophila trachea; tube dilation is suppressed by reduction of NSF2
Title: Focal defects in single-celled tubes mutant for Cerebral cavernous malformation 3, GCKIII, or NSF2.

The following GckIII gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GckIII cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05193	NM_142339.3 Latest version!	Drosophila melanogaster germinal centre kinase III (GckIII), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GckIII ( NM_142339.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650596.1 Drosophila melanogaster germinal centre kinase III (GckIII), mRNA.

Price & Availability↑

CloneID	OFa05193
Clone ID Related Accession (Same CDS sequence)	NM_142339.3
Accession Version	NM_142339.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	germinal centre kinase III
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142339.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCTGGA CGGAGAAAGT CGATCCAGAG CTCATCTTCA CCAAGCAGGA GCGCATCGGC 
AAGGGCTCCT TCGGCGAGGT GTTCAAGGGC ATCGACAACC GCACCCAGCA AGTGGTGGCC 
ATCAAGATCA TCGATCTGGA GGAGGCCGAA GATGAGATCG ACGACATCCA GCAGGAGATC 
ATGGTGCTGT CGCAGTGCGA CTCGCCGTAC GTGACGAAGT ACTATGGCAG CTTTCTTAAG 
GGCACCAAGT TATGGATCAT CATGGAGTAC CTGGGCGGTG GATCCGCCTT GGACCTCATG 
AAGGCCGGCT CCTTCGAGGA AATGCACATC GGGATCATAT TGCGCGAAGT GCTTAAGGGC 
CTGGACTACC TGCACTCGGA GCGCAAGCTG CATCGCGACA TCAAGGCGGC CAATGTTCTG 
TTGAGCGAAC AGGGCGATGT CAAGCTGGCG GACTTTGGAG TCGCTGGCCA GCTGACCAAC 
ACCACATCCA AGAGGAACAC CTTCGTGGGC ACGCCCTTTT GGATGGCGCC CGAGGTGATC 
AAGCAGTCAC AGTACGATGC CAAGGCGGAC ATCTGGTCGT TGGGCATCAC GGCTATCGAA 
CTGGCCAAGG GTGAGCCGCC CAATTCGGAG CTGCATCCGA TGCGCGTGCT CTTCCTCATC 
CCCAAGAACA ATCCACCCCA GCTGACGGGC AGCTATACCA AGTCTTTTAA GGACTTTGTG 
GAGGCCTGCC TAAACAAGGA CCCGGAGAAC AGGCCCACGG CCAAGGAGCT GCTCAAGTAC 
CCGTTCATCA AGAAGGCCAA GAAGAACGCC TATCTGATTG ACCTGATCGA CCGCTTCAAG 
AAGTGGAAGG TCTCCAAGGG CGACGAAAGT GAGACGGAGT CTGAGAACTC TGACTCGGAC 
TCGGACGCCA AGCAGGGCGG CAGCAGCCAG GATGAGCCCT GGATCATGAC CGTCAAGGGC 
CTGCATATCA ACAGTGCTCC GCGGGCGGGT GTCAACAGCT TCCAGTTGCA GGAGCACGAG 
CTAACCATGC AGAAGCTGAT GCAGACCACA CACTCGCCAG CCGGCGGTGG TGGCGTGCAA 
GCGTCCCCTG CTGCAAATGC ATTATCGTCC TCTGTGTCCG CCGTGGCGGC GTCAGCTTCC 
TCCGTATCGG TCATTACAGC CAACGAAGGT GTCTCCTCTG CATCCGCCCT GCCCCCCCAG 
AACAGTCACG TCCACAACCA CAACCACAAC AACATTAACA ACAATAACAT CAGAAACTTG 
AGCAACAAGC ATGCCACCAC CACCATGCTG AACGCAGCTC CTCCGCCGAG CAGCGTCACA 
AGCTCGTCGT CGGCCAGTGA GCTGCAGCAG AAGCGTTCCA GCTCGAAGCC GGAGCTGCGC 
CAGTCGCGAT CCGCCGCCCC TCTGGATCAA GTAGAGGATC GCCGTGCCTC TTTGGGCCTG 
CCATTGCAGT CGTCGGTTCC GACTCCATCG ACGGCTGAGC AGCAGCAACA GCGCCGCAGC 
AGCCAACGTG AATCATTCCA TGCAAATAAC GCCGCGCTGG TTGCCAGCTG CAGCAGCAGT 
AGCAACAACC ACAGCCACGA GGAGCATGTG GCCACCAACC ATGCCAGCCA TGTCAAGCAC 
TCTGGGGCGG GACAACGCCT AGTCCAAACG AACAGCGCCT GTCTGAACAA CTTCCTCTTC 
CCCGTGCTGA GCGACATCCA GCGAAAGTAC TCCGGCGGAG GATCTGCAGG AAGCGGAGGA 
TCAGGCGGCC GTAACATAGG CGTCGGCAGC GACATCGGCG ACCTGAAGTC TGTGTTCGAG 
CTGGCCGAAC GTTCCAGTCC GGGAGTGAGC GACCTATTTA TGAAAGAGCT GATCCACATG 
CTCGTTCCTG GTTACTCGGA GACGCGAATC AATACGATCA TGGACCGCGC CATACGCAAC 
CGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650596.1
CDS	286..2214
Translation	MSWTEKVDPELIFTKQERIGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIDDIQQEIMVLSQCDSPYVTKYYGSFLKGTKLWIIMEYLGGGSALDLMKAGSFEEMHIGIILREVLKGLDYLHSERKLHRDIKAANVLLSEQGDVKLADFGVAGQLTNTTSKRNTFVGTPFWMAPEVIKQSQYDAKADIWSLGITAIELAKGEPPNSELHPMRVLFLIPKNNPPQLTGSYTKSFKDFVEACLNKDPENRPTAKELLKYPFIKKAKKNAYLIDLIDRFKKWKVSKGDESETESENSDSDSDAKQGGSSQDEPWIMTVKGLHINSAPRAGVNSFQLQEHELTMQKLMQTTHSPAGGGGVQASPAANALSSSVSAVAASASSVSVITANEGVSSASALPPQNSHVHNHNHNNINNNNIRNLSNKHATTTMLNAAPPPSSVTSSSSASELQQKRSSSKPELRQSRSAAPLDQVEDRRASLGLPLQSSVPTPSTAEQQQQRRSSQRESFHANNAALVASCSSSSNNHSHEEHVATNHASHVKHSGAGQRLVQTNSACLNNFLFPVLSDIQRKYSGGGSAGSGGSGGRNIGVGSDIGDLKSVFELAERSSPGVSDLFMKELIHMLVPGYSETRINTIMDRAIRNRK

Target ORF information:

RefSeq Version	NM_142339.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster germinal centre kinase III (GckIII), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142339.3

ORF Insert Sequence:

ATGTCCTGGA CGGAGAAAGT CGATCCAGAG CTCATCTTCA CCAAGCAGGA GCGCATCGGC 
AAGGGCTCCT TCGGCGAGGT GTTCAAGGGC ATCGACAACC GCACCCAGCA AGTGGTGGCC 
ATCAAGATCA TCGATCTGGA GGAGGCCGAA GATGAGATCG ACGACATCCA GCAGGAGATC 
ATGGTGCTGT CGCAGTGCGA CTCGCCGTAC GTGACGAAGT ACTATGGCAG CTTTCTTAAG 
GGCACCAAGT TATGGATCAT CATGGAGTAC CTGGGCGGTG GATCCGCCTT GGACCTCATG 
AAGGCCGGCT CCTTCGAGGA AATGCACATC GGGATCATAT TGCGCGAAGT GCTTAAGGGC 
CTGGACTACC TGCACTCGGA GCGCAAGCTG CATCGCGACA TCAAGGCGGC CAATGTTCTG 
TTGAGCGAAC AGGGCGATGT CAAGCTGGCG GACTTTGGAG TCGCTGGCCA GCTGACCAAC 
ACCACATCCA AGAGGAACAC CTTCGTGGGC ACGCCCTTTT GGATGGCGCC CGAGGTGATC 
AAGCAGTCAC AGTACGATGC CAAGGCGGAC ATCTGGTCGT TGGGCATCAC GGCTATCGAA 
CTGGCCAAGG GTGAGCCGCC CAATTCGGAG CTGCATCCGA TGCGCGTGCT CTTCCTCATC 
CCCAAGAACA ATCCACCCCA GCTGACGGGC AGCTATACCA AGTCTTTTAA GGACTTTGTG 
GAGGCCTGCC TAAACAAGGA CCCGGAGAAC AGGCCCACGG CCAAGGAGCT GCTCAAGTAC 
CCGTTCATCA AGAAGGCCAA GAAGAACGCC TATCTGATTG ACCTGATCGA CCGCTTCAAG 
AAGTGGAAGG TCTCCAAGGG CGACGAAAGT GAGACGGAGT CTGAGAACTC TGACTCGGAC 
TCGGACGCCA AGCAGGGCGG CAGCAGCCAG GATGAGCCCT GGATCATGAC CGTCAAGGGC 
CTGCATATCA ACAGTGCTCC GCGGGCGGGT GTCAACAGCT TCCAGTTGCA GGAGCACGAG 
CTAACCATGC AGAAGCTGAT GCAGACCACA CACTCGCCAG CCGGCGGTGG TGGCGTGCAA 
GCGTCCCCTG CTGCAAATGC ATTATCGTCC TCTGTGTCCG CCGTGGCGGC GTCAGCTTCC 
TCCGTATCGG TCATTACAGC CAACGAAGGT GTCTCCTCTG CATCCGCCCT GCCCCCCCAG 
AACAGTCACG TCCACAACCA CAACCACAAC AACATTAACA ACAATAACAT CAGAAACTTG 
AGCAACAAGC ATGCCACCAC CACCATGCTG AACGCAGCTC CTCCGCCGAG CAGCGTCACA 
AGCTCGTCGT CGGCCAGTGA GCTGCAGCAG AAGCGTTCCA GCTCGAAGCC GGAGCTGCGC 
CAGTCGCGAT CCGCCGCCCC TCTGGATCAA GTAGAGGATC GCCGTGCCTC TTTGGGCCTG 
CCATTGCAGT CGTCGGTTCC GACTCCATCG ACGGCTGAGC AGCAGCAACA GCGCCGCAGC 
AGCCAACGTG AATCATTCCA TGCAAATAAC GCCGCGCTGG TTGCCAGCTG CAGCAGCAGT 
AGCAACAACC ACAGCCACGA GGAGCATGTG GCCACCAACC ATGCCAGCCA TGTCAAGCAC 
TCTGGGGCGG GACAACGCCT AGTCCAAACG AACAGCGCCT GTCTGAACAA CTTCCTCTTC 
CCCGTGCTGA GCGACATCCA GCGAAAGTAC TCCGGCGGAG GATCTGCAGG AAGCGGAGGA 
TCAGGCGGCC GTAACATAGG CGTCGGCAGC GACATCGGCG ACCTGAAGTC TGTGTTCGAG 
CTGGCCGAAC GTTCCAGTCC GGGAGTGAGC GACCTATTTA TGAAAGAGCT GATCCACATG 
CTCGTTCCTG GTTACTCGGA GACGCGAATC AATACGATCA TGGACCGCGC CATACGCAAC 
CGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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GckIII cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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