Dad cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dad
Entrez Gene ID	42059
Full Name	Daughters against dpp
Synonyms	1883,CG5201,DAD,Dad1,Dmel\CG5201,EP(3)3196,EP3196,anon-EST:fe1F5,dad,l(3)j1E4
General protein information	Preferred Names Daughters against dpp Names CG5201 gene product from transcript CG5201-RA CG5201-PA CG5201-PB CG5201-PC Dad-PA Dad-PB Dad-PC anon-fast-evolving-1F5 daughter against dpp daughters against Dpp daughters against dpp
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89E11-89E11

mRNA and Protein(s)

mRNA	Protein	Name
NM_057912.4	NP_477260.1	daughters against dpp, isoform A
NM_001014629.2	NP_001014629.1	daughters against dpp, isoform C
NM_169740.2	NP_732196.1	daughters against dpp, isoform B

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	smad6	XP_002938398.1
Drosophila melanogaster (fruit fly)	Dad	NP_477260.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dad and Dpp activity is dynamically regulated in the adult Drosophila middle midgut. Dynamic regulation of Dad is required for gastric stem cell differentiation.
Title: Ubx dynamically regulates Dpp signaling by repressing Dad expression during copper cell regeneration in the adult Drosophila midgut.

Robustness of the Dpp morphogen activity gradient depends on negative feedback regulation by the inhibitory Smad, Dad.
Title: Robustness of the Dpp morphogen activity gradient depends on negative feedback regulation by the inhibitory Smad, Dad.

Dad inhibited Saxophone and Thickveins but not Baboon.
Title: Specificity of the inhibitory effects of Dad on TGF-beta family type I receptors, Thickveins, Saxophone, and Baboon in Drosophila.

Dad is an inhibitor of dpp.
Title: The role of Dpp and its inhibitors during eggshell patterning in Drosophila.

The following Dad gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dad cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05187	NM_057912.4 Latest version!	Drosophila melanogaster daughters against dpp, transcript variant A (Dad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05187	NM_001014629.2 Latest version!	Drosophila melanogaster daughters against dpp, transcript variant C (Dad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05187	NM_169740.2 Latest version!	Drosophila melanogaster daughters against dpp, transcript variant B (Dad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dad ( NM_057912.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477260.1 Drosophila melanogaster daughters against dpp, transcript variant A (Dad), mRNA.

Price & Availability↑

CloneID	OFa05187
Clone ID Related Accession (Same CDS sequence)	NM_057912.4 , NM_001014629.2 , NM_169740.2
Accession Version	NM_057912.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	daughters against dpp, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057912.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATATTCC CAAGAGAAAA GAAGGTGTTA TGGCGTTATG CGTCCAGCAA CAATCCGAGC 
AATGGGGTGT CAGCAGCACC GCCCGCTCAG CCACCACGGC CGCCTCCCCC TCCACATCGA 
CCCCGCCCTC ATCAGTGTAC TCCAAGCTTT GGTTATTCCT GCAACGAAGA GGACTCCTTG 
GCAATGCGAC AGACGCCGCT GCCGCCTTAC AGTTCGATAG CCTGCGGGAT GGACTGCTCC 
AGCTCAAACT CCAGCTCCTG TGGCCAGAGT TTGAGTCTGA GTCAGGGGCA AGGCCACAAC 
AACAACAACA GCCATCCGTA CCGCCGTCTG CCAAATCACA TGGATGTGTT GCCGCCGCCT 
CCGTCAGCGT GCGACCGCTG CTGTACCGCT CCTGGTTACA GCTGCGCAAG CTCCTGCGAC 
GATATGTTGA TCGACGGAAG CGATCTGGAT CAGGATCGGA GTCCGGATCA GGGACAGGTT 
CAGCCGGTGG ACCGCCGGAT GATAAGTGCC ACCACCTTTA CGACCATGTT TCGGAAATGC 
TGCGGCGGCG CAACGGAGTC CACCAGCGGA TCCACACTTA CGATTCCAGT CAGCACGTCC 
AGAGCCACCG CTCATCCTCC CCAAACGCAA GCCCAGAATG GCAAACGATT TCGCGAGGAT 
TTCGAAGCGC TAATGAAGCA ACTCAAGCGC AAGCAGAGAA ACGAACTTCT GCTTGCAGTC 
AAGAGTCGCC TAGATCCGCC GACCAAGACG CAGCGCGATG TCGTGGAACC CACCACCACC 
ACCGCACCCA CCTACCTGCA GTGCATCCTG ATACCGTGCA AAACGCAAAC GGTTTGGGAG 
CCGCACGTGA CCGCCAGTCG CCTGTTTTTC TGGCGGGAAT TGTGGAACGC CAAGGAACTG 
AAGCGACTGC CCACTTGTCC CGCCGCCCGC GACTGCATCT ACATGTGCTG CAATCCGCTG 
CATTGGTTTC GCATCCTGCA CCAGCCCGAA ACAGAATCCC CAACACCGCC GTACCAACGC 
TCAAAAATGC TGAGACTGAA AGATGCAGAT TTCGAAGAAG ACTCGCAGAA CGATGCAAAG 
TCGGCAGCGC TCAGCACGTG GAGTGCCGAA AGCACCAGCA TTTCGAACAT CTACAAGCCG 
GCTCTCTACG AGTCGGTTAC CACTGATGGA AAAGATCATA ACATCAACAG TCAGGTGTGG 
TGCCAAATCG CCTACTGGGA GATGGCCCAC CGCGTCGGGG AGTTCTTTCA CGCCAAAACG 
AATGCAGTCA ATATCTACAC GGATGGGATT GTGGCCAGTG AGGTGGACAG CATGTGCCTA 
CGTGACCTCA CACCAGCAGG CAATCAGATC CACTCGGTGG TGCCAAAAGC GCGTCATACG 
GTCGGATTGG GGGTCACATT GAGTCTGGAG AACGGCGATG TTTGGATCTA CAATCGTGGG 
AATACAACCA TTTTTGTCGA TTCACCCACC CTGTCCGAAA ATCTGGACCG AGTGTGCAAA 
GTGATGCCGG GCTATTGTCT GAAGGCCTTT GAAACCAATA GGGCTGAACT TCTGAGCATG 
AGAGATCACG GACATCATCC CATGGGACCC GTCGACTACT TCAGCATTAA GATCAGCTTT 
GGCAAGGGCT GGGGACGCGA CTACAAAAGA CAGGATATCA TGGGCTGCCC CTGCTGGCTG 
GAGGTTCACT TTAGTCATCT GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477260.1
CDS	788..2494
Translation	MIFPREKKVLWRYASSNNPSNGVSAAPPAQPPRPPPPPHRPRPHQCTPSFGYSCNEEDSLAMRQTPLPPYSSIACGMDCSSSNSSSCGQSLSLSQGQGHNNNNSHPYRRLPNHMDVLPPPPSACDRCCTAPGYSCASSCDDMLIDGSDLDQDRSPDQGQVQPVDRRMISATTFTTMFRKCCGGATESTSGSTLTIPVSTSRATAHPPQTQAQNGKRFREDFEALMKQLKRKQRNELLLAVKSRLDPPTKTQRDVVEPTTTTAPTYLQCILIPCKTQTVWEPHVTASRLFFWRELWNAKELKRLPTCPAARDCIYMCCNPLHWFRILHQPETESPTPPYQRSKMLRLKDADFEEDSQNDAKSAALSTWSAESTSISNIYKPALYESVTTDGKDHNINSQVWCQIAYWEMAHRVGEFFHAKTNAVNIYTDGIVASEVDSMCLRDLTPAGNQIHSVVPKARHTVGLGVTLSLENGDVWIYNRGNTTIFVDSPTLSENLDRVCKVMPGYCLKAFETNRAELLSMRDHGHHPMGPVDYFSIKISFGKGWGRDYKRQDIMGCPCWLEVHFSHLR

Target ORF information:

RefSeq Version	NM_057912.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster daughters against dpp, transcript variant A (Dad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057912.4

ORF Insert Sequence:

ATGATATTCC CAAGAGAAAA GAAGGTGTTA TGGCGTTATG CGTCCAGCAA CAATCCGAGC 
AATGGGGTGT CAGCAGCACC GCCCGCTCAG CCACCACGGC CGCCTCCCCC TCCACATCGA 
CCCCGCCCTC ATCAGTGTAC TCCAAGCTTT GGTTATTCCT GCAACGAAGA GGACTCCTTG 
GCAATGCGAC AGACGCCGCT GCCGCCTTAC AGTTCGATAG CCTGCGGGAT GGACTGCTCC 
AGCTCAAACT CCAGCTCCTG TGGCCAGAGT TTGAGTCTGA GTCAGGGGCA AGGCCACAAC 
AACAACAACA GCCATCCGTA CCGCCGTCTG CCAAATCACA TGGATGTGTT GCCGCCGCCT 
CCGTCAGCGT GCGACCGCTG CTGTACCGCT CCTGGTTACA GCTGCGCAAG CTCCTGCGAC 
GATATGTTGA TCGACGGAAG CGATCTGGAT CAGGATCGGA GTCCGGATCA GGGACAGGTT 
CAGCCGGTGG ACCGCCGGAT GATAAGTGCC ACCACCTTTA CGACCATGTT TCGGAAATGC 
TGCGGCGGCG CAACGGAGTC CACCAGCGGA TCCACACTTA CGATTCCAGT CAGCACGTCC 
AGAGCCACCG CTCATCCTCC CCAAACGCAA GCCCAGAATG GCAAACGATT TCGCGAGGAT 
TTCGAAGCGC TAATGAAGCA ACTCAAGCGC AAGCAGAGAA ACGAACTTCT GCTTGCAGTC 
AAGAGTCGCC TAGATCCGCC GACCAAGACG CAGCGCGATG TCGTGGAACC CACCACCACC 
ACCGCACCCA CCTACCTGCA GTGCATCCTG ATACCGTGCA AAACGCAAAC GGTTTGGGAG 
CCGCACGTGA CCGCCAGTCG CCTGTTTTTC TGGCGGGAAT TGTGGAACGC CAAGGAACTG 
AAGCGACTGC CCACTTGTCC CGCCGCCCGC GACTGCATCT ACATGTGCTG CAATCCGCTG 
CATTGGTTTC GCATCCTGCA CCAGCCCGAA ACAGAATCCC CAACACCGCC GTACCAACGC 
TCAAAAATGC TGAGACTGAA AGATGCAGAT TTCGAAGAAG ACTCGCAGAA CGATGCAAAG 
TCGGCAGCGC TCAGCACGTG GAGTGCCGAA AGCACCAGCA TTTCGAACAT CTACAAGCCG 
GCTCTCTACG AGTCGGTTAC CACTGATGGA AAAGATCATA ACATCAACAG TCAGGTGTGG 
TGCCAAATCG CCTACTGGGA GATGGCCCAC CGCGTCGGGG AGTTCTTTCA CGCCAAAACG 
AATGCAGTCA ATATCTACAC GGATGGGATT GTGGCCAGTG AGGTGGACAG CATGTGCCTA 
CGTGACCTCA CACCAGCAGG CAATCAGATC CACTCGGTGG TGCCAAAAGC GCGTCATACG 
GTCGGATTGG GGGTCACATT GAGTCTGGAG AACGGCGATG TTTGGATCTA CAATCGTGGG 
AATACAACCA TTTTTGTCGA TTCACCCACC CTGTCCGAAA ATCTGGACCG AGTGTGCAAA 
GTGATGCCGG GCTATTGTCT GAAGGCCTTT GAAACCAATA GGGCTGAACT TCTGAGCATG 
AGAGATCACG GACATCATCC CATGGGACCC GTCGACTACT TCAGCATTAA GATCAGCTTT 
GGCAAGGGCT GGGGACGCGA CTACAAAAGA CAGGATATCA TGGGCTGCCC CTGCTGGCTG 
GAGGTTCACT TTAGTCATCT GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dad ( NM_001014629.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014629.1 Drosophila melanogaster daughters against dpp, transcript variant C (Dad), mRNA.

Price & Availability↑

CloneID	OFa05187
Clone ID Related Accession (Same CDS sequence)	NM_057912.4 , NM_001014629.2 , NM_169740.2
Accession Version	NM_001014629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	daughters against dpp, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001014629.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATATTCC CAAGAGAAAA GAAGGTGTTA TGGCGTTATG CGTCCAGCAA CAATCCGAGC 
AATGGGGTGT CAGCAGCACC GCCCGCTCAG CCACCACGGC CGCCTCCCCC TCCACATCGA 
CCCCGCCCTC ATCAGTGTAC TCCAAGCTTT GGTTATTCCT GCAACGAAGA GGACTCCTTG 
GCAATGCGAC AGACGCCGCT GCCGCCTTAC AGTTCGATAG CCTGCGGGAT GGACTGCTCC 
AGCTCAAACT CCAGCTCCTG TGGCCAGAGT TTGAGTCTGA GTCAGGGGCA AGGCCACAAC 
AACAACAACA GCCATCCGTA CCGCCGTCTG CCAAATCACA TGGATGTGTT GCCGCCGCCT 
CCGTCAGCGT GCGACCGCTG CTGTACCGCT CCTGGTTACA GCTGCGCAAG CTCCTGCGAC 
GATATGTTGA TCGACGGAAG CGATCTGGAT CAGGATCGGA GTCCGGATCA GGGACAGGTT 
CAGCCGGTGG ACCGCCGGAT GATAAGTGCC ACCACCTTTA CGACCATGTT TCGGAAATGC 
TGCGGCGGCG CAACGGAGTC CACCAGCGGA TCCACACTTA CGATTCCAGT CAGCACGTCC 
AGAGCCACCG CTCATCCTCC CCAAACGCAA GCCCAGAATG GCAAACGATT TCGCGAGGAT 
TTCGAAGCGC TAATGAAGCA ACTCAAGCGC AAGCAGAGAA ACGAACTTCT GCTTGCAGTC 
AAGAGTCGCC TAGATCCGCC GACCAAGACG CAGCGCGATG TCGTGGAACC CACCACCACC 
ACCGCACCCA CCTACCTGCA GTGCATCCTG ATACCGTGCA AAACGCAAAC GGTTTGGGAG 
CCGCACGTGA CCGCCAGTCG CCTGTTTTTC TGGCGGGAAT TGTGGAACGC CAAGGAACTG 
AAGCGACTGC CCACTTGTCC CGCCGCCCGC GACTGCATCT ACATGTGCTG CAATCCGCTG 
CATTGGTTTC GCATCCTGCA CCAGCCCGAA ACAGAATCCC CAACACCGCC GTACCAACGC 
TCAAAAATGC TGAGACTGAA AGATGCAGAT TTCGAAGAAG ACTCGCAGAA CGATGCAAAG 
TCGGCAGCGC TCAGCACGTG GAGTGCCGAA AGCACCAGCA TTTCGAACAT CTACAAGCCG 
GCTCTCTACG AGTCGGTTAC CACTGATGGA AAAGATCATA ACATCAACAG TCAGGTGTGG 
TGCCAAATCG CCTACTGGGA GATGGCCCAC CGCGTCGGGG AGTTCTTTCA CGCCAAAACG 
AATGCAGTCA ATATCTACAC GGATGGGATT GTGGCCAGTG AGGTGGACAG CATGTGCCTA 
CGTGACCTCA CACCAGCAGG CAATCAGATC CACTCGGTGG TGCCAAAAGC GCGTCATACG 
GTCGGATTGG GGGTCACATT GAGTCTGGAG AACGGCGATG TTTGGATCTA CAATCGTGGG 
AATACAACCA TTTTTGTCGA TTCACCCACC CTGTCCGAAA ATCTGGACCG AGTGTGCAAA 
GTGATGCCGG GCTATTGTCT GAAGGCCTTT GAAACCAATA GGGCTGAACT TCTGAGCATG 
AGAGATCACG GACATCATCC CATGGGACCC GTCGACTACT TCAGCATTAA GATCAGCTTT 
GGCAAGGGCT GGGGACGCGA CTACAAAAGA CAGGATATCA TGGGCTGCCC CTGCTGGCTG 
GAGGTTCACT TTAGTCATCT GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014629.1
CDS	772..2478
Translation	MIFPREKKVLWRYASSNNPSNGVSAAPPAQPPRPPPPPHRPRPHQCTPSFGYSCNEEDSLAMRQTPLPPYSSIACGMDCSSSNSSSCGQSLSLSQGQGHNNNNSHPYRRLPNHMDVLPPPPSACDRCCTAPGYSCASSCDDMLIDGSDLDQDRSPDQGQVQPVDRRMISATTFTTMFRKCCGGATESTSGSTLTIPVSTSRATAHPPQTQAQNGKRFREDFEALMKQLKRKQRNELLLAVKSRLDPPTKTQRDVVEPTTTTAPTYLQCILIPCKTQTVWEPHVTASRLFFWRELWNAKELKRLPTCPAARDCIYMCCNPLHWFRILHQPETESPTPPYQRSKMLRLKDADFEEDSQNDAKSAALSTWSAESTSISNIYKPALYESVTTDGKDHNINSQVWCQIAYWEMAHRVGEFFHAKTNAVNIYTDGIVASEVDSMCLRDLTPAGNQIHSVVPKARHTVGLGVTLSLENGDVWIYNRGNTTIFVDSPTLSENLDRVCKVMPGYCLKAFETNRAELLSMRDHGHHPMGPVDYFSIKISFGKGWGRDYKRQDIMGCPCWLEVHFSHLR

Target ORF information:

RefSeq Version	NM_001014629.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster daughters against dpp, transcript variant C (Dad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014629.2

ORF Insert Sequence:

ATGATATTCC CAAGAGAAAA GAAGGTGTTA TGGCGTTATG CGTCCAGCAA CAATCCGAGC 
AATGGGGTGT CAGCAGCACC GCCCGCTCAG CCACCACGGC CGCCTCCCCC TCCACATCGA 
CCCCGCCCTC ATCAGTGTAC TCCAAGCTTT GGTTATTCCT GCAACGAAGA GGACTCCTTG 
GCAATGCGAC AGACGCCGCT GCCGCCTTAC AGTTCGATAG CCTGCGGGAT GGACTGCTCC 
AGCTCAAACT CCAGCTCCTG TGGCCAGAGT TTGAGTCTGA GTCAGGGGCA AGGCCACAAC 
AACAACAACA GCCATCCGTA CCGCCGTCTG CCAAATCACA TGGATGTGTT GCCGCCGCCT 
CCGTCAGCGT GCGACCGCTG CTGTACCGCT CCTGGTTACA GCTGCGCAAG CTCCTGCGAC 
GATATGTTGA TCGACGGAAG CGATCTGGAT CAGGATCGGA GTCCGGATCA GGGACAGGTT 
CAGCCGGTGG ACCGCCGGAT GATAAGTGCC ACCACCTTTA CGACCATGTT TCGGAAATGC 
TGCGGCGGCG CAACGGAGTC CACCAGCGGA TCCACACTTA CGATTCCAGT CAGCACGTCC 
AGAGCCACCG CTCATCCTCC CCAAACGCAA GCCCAGAATG GCAAACGATT TCGCGAGGAT 
TTCGAAGCGC TAATGAAGCA ACTCAAGCGC AAGCAGAGAA ACGAACTTCT GCTTGCAGTC 
AAGAGTCGCC TAGATCCGCC GACCAAGACG CAGCGCGATG TCGTGGAACC CACCACCACC 
ACCGCACCCA CCTACCTGCA GTGCATCCTG ATACCGTGCA AAACGCAAAC GGTTTGGGAG 
CCGCACGTGA CCGCCAGTCG CCTGTTTTTC TGGCGGGAAT TGTGGAACGC CAAGGAACTG 
AAGCGACTGC CCACTTGTCC CGCCGCCCGC GACTGCATCT ACATGTGCTG CAATCCGCTG 
CATTGGTTTC GCATCCTGCA CCAGCCCGAA ACAGAATCCC CAACACCGCC GTACCAACGC 
TCAAAAATGC TGAGACTGAA AGATGCAGAT TTCGAAGAAG ACTCGCAGAA CGATGCAAAG 
TCGGCAGCGC TCAGCACGTG GAGTGCCGAA AGCACCAGCA TTTCGAACAT CTACAAGCCG 
GCTCTCTACG AGTCGGTTAC CACTGATGGA AAAGATCATA ACATCAACAG TCAGGTGTGG 
TGCCAAATCG CCTACTGGGA GATGGCCCAC CGCGTCGGGG AGTTCTTTCA CGCCAAAACG 
AATGCAGTCA ATATCTACAC GGATGGGATT GTGGCCAGTG AGGTGGACAG CATGTGCCTA 
CGTGACCTCA CACCAGCAGG CAATCAGATC CACTCGGTGG TGCCAAAAGC GCGTCATACG 
GTCGGATTGG GGGTCACATT GAGTCTGGAG AACGGCGATG TTTGGATCTA CAATCGTGGG 
AATACAACCA TTTTTGTCGA TTCACCCACC CTGTCCGAAA ATCTGGACCG AGTGTGCAAA 
GTGATGCCGG GCTATTGTCT GAAGGCCTTT GAAACCAATA GGGCTGAACT TCTGAGCATG 
AGAGATCACG GACATCATCC CATGGGACCC GTCGACTACT TCAGCATTAA GATCAGCTTT 
GGCAAGGGCT GGGGACGCGA CTACAAAAGA CAGGATATCA TGGGCTGCCC CTGCTGGCTG 
GAGGTTCACT TTAGTCATCT GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dad ( NM_169740.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732196.1 Drosophila melanogaster daughters against dpp, transcript variant B (Dad), mRNA.

Price & Availability↑

CloneID	OFa05187
Clone ID Related Accession (Same CDS sequence)	NM_057912.4 , NM_001014629.2 , NM_169740.2
Accession Version	NM_169740.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	daughters against dpp, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169740.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATATTCC CAAGAGAAAA GAAGGTGTTA TGGCGTTATG CGTCCAGCAA CAATCCGAGC 
AATGGGGTGT CAGCAGCACC GCCCGCTCAG CCACCACGGC CGCCTCCCCC TCCACATCGA 
CCCCGCCCTC ATCAGTGTAC TCCAAGCTTT GGTTATTCCT GCAACGAAGA GGACTCCTTG 
GCAATGCGAC AGACGCCGCT GCCGCCTTAC AGTTCGATAG CCTGCGGGAT GGACTGCTCC 
AGCTCAAACT CCAGCTCCTG TGGCCAGAGT TTGAGTCTGA GTCAGGGGCA AGGCCACAAC 
AACAACAACA GCCATCCGTA CCGCCGTCTG CCAAATCACA TGGATGTGTT GCCGCCGCCT 
CCGTCAGCGT GCGACCGCTG CTGTACCGCT CCTGGTTACA GCTGCGCAAG CTCCTGCGAC 
GATATGTTGA TCGACGGAAG CGATCTGGAT CAGGATCGGA GTCCGGATCA GGGACAGGTT 
CAGCCGGTGG ACCGCCGGAT GATAAGTGCC ACCACCTTTA CGACCATGTT TCGGAAATGC 
TGCGGCGGCG CAACGGAGTC CACCAGCGGA TCCACACTTA CGATTCCAGT CAGCACGTCC 
AGAGCCACCG CTCATCCTCC CCAAACGCAA GCCCAGAATG GCAAACGATT TCGCGAGGAT 
TTCGAAGCGC TAATGAAGCA ACTCAAGCGC AAGCAGAGAA ACGAACTTCT GCTTGCAGTC 
AAGAGTCGCC TAGATCCGCC GACCAAGACG CAGCGCGATG TCGTGGAACC CACCACCACC 
ACCGCACCCA CCTACCTGCA GTGCATCCTG ATACCGTGCA AAACGCAAAC GGTTTGGGAG 
CCGCACGTGA CCGCCAGTCG CCTGTTTTTC TGGCGGGAAT TGTGGAACGC CAAGGAACTG 
AAGCGACTGC CCACTTGTCC CGCCGCCCGC GACTGCATCT ACATGTGCTG CAATCCGCTG 
CATTGGTTTC GCATCCTGCA CCAGCCCGAA ACAGAATCCC CAACACCGCC GTACCAACGC 
TCAAAAATGC TGAGACTGAA AGATGCAGAT TTCGAAGAAG ACTCGCAGAA CGATGCAAAG 
TCGGCAGCGC TCAGCACGTG GAGTGCCGAA AGCACCAGCA TTTCGAACAT CTACAAGCCG 
GCTCTCTACG AGTCGGTTAC CACTGATGGA AAAGATCATA ACATCAACAG TCAGGTGTGG 
TGCCAAATCG CCTACTGGGA GATGGCCCAC CGCGTCGGGG AGTTCTTTCA CGCCAAAACG 
AATGCAGTCA ATATCTACAC GGATGGGATT GTGGCCAGTG AGGTGGACAG CATGTGCCTA 
CGTGACCTCA CACCAGCAGG CAATCAGATC CACTCGGTGG TGCCAAAAGC GCGTCATACG 
GTCGGATTGG GGGTCACATT GAGTCTGGAG AACGGCGATG TTTGGATCTA CAATCGTGGG 
AATACAACCA TTTTTGTCGA TTCACCCACC CTGTCCGAAA ATCTGGACCG AGTGTGCAAA 
GTGATGCCGG GCTATTGTCT GAAGGCCTTT GAAACCAATA GGGCTGAACT TCTGAGCATG 
AGAGATCACG GACATCATCC CATGGGACCC GTCGACTACT TCAGCATTAA GATCAGCTTT 
GGCAAGGGCT GGGGACGCGA CTACAAAAGA CAGGATATCA TGGGCTGCCC CTGCTGGCTG 
GAGGTTCACT TTAGTCATCT GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732196.1
CDS	571..2277
Translation	MIFPREKKVLWRYASSNNPSNGVSAAPPAQPPRPPPPPHRPRPHQCTPSFGYSCNEEDSLAMRQTPLPPYSSIACGMDCSSSNSSSCGQSLSLSQGQGHNNNNSHPYRRLPNHMDVLPPPPSACDRCCTAPGYSCASSCDDMLIDGSDLDQDRSPDQGQVQPVDRRMISATTFTTMFRKCCGGATESTSGSTLTIPVSTSRATAHPPQTQAQNGKRFREDFEALMKQLKRKQRNELLLAVKSRLDPPTKTQRDVVEPTTTTAPTYLQCILIPCKTQTVWEPHVTASRLFFWRELWNAKELKRLPTCPAARDCIYMCCNPLHWFRILHQPETESPTPPYQRSKMLRLKDADFEEDSQNDAKSAALSTWSAESTSISNIYKPALYESVTTDGKDHNINSQVWCQIAYWEMAHRVGEFFHAKTNAVNIYTDGIVASEVDSMCLRDLTPAGNQIHSVVPKARHTVGLGVTLSLENGDVWIYNRGNTTIFVDSPTLSENLDRVCKVMPGYCLKAFETNRAELLSMRDHGHHPMGPVDYFSIKISFGKGWGRDYKRQDIMGCPCWLEVHFSHLR

Target ORF information:

RefSeq Version	NM_169740.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster daughters against dpp, transcript variant B (Dad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169740.2

ORF Insert Sequence:

ATGATATTCC CAAGAGAAAA GAAGGTGTTA TGGCGTTATG CGTCCAGCAA CAATCCGAGC 
AATGGGGTGT CAGCAGCACC GCCCGCTCAG CCACCACGGC CGCCTCCCCC TCCACATCGA 
CCCCGCCCTC ATCAGTGTAC TCCAAGCTTT GGTTATTCCT GCAACGAAGA GGACTCCTTG 
GCAATGCGAC AGACGCCGCT GCCGCCTTAC AGTTCGATAG CCTGCGGGAT GGACTGCTCC 
AGCTCAAACT CCAGCTCCTG TGGCCAGAGT TTGAGTCTGA GTCAGGGGCA AGGCCACAAC 
AACAACAACA GCCATCCGTA CCGCCGTCTG CCAAATCACA TGGATGTGTT GCCGCCGCCT 
CCGTCAGCGT GCGACCGCTG CTGTACCGCT CCTGGTTACA GCTGCGCAAG CTCCTGCGAC 
GATATGTTGA TCGACGGAAG CGATCTGGAT CAGGATCGGA GTCCGGATCA GGGACAGGTT 
CAGCCGGTGG ACCGCCGGAT GATAAGTGCC ACCACCTTTA CGACCATGTT TCGGAAATGC 
TGCGGCGGCG CAACGGAGTC CACCAGCGGA TCCACACTTA CGATTCCAGT CAGCACGTCC 
AGAGCCACCG CTCATCCTCC CCAAACGCAA GCCCAGAATG GCAAACGATT TCGCGAGGAT 
TTCGAAGCGC TAATGAAGCA ACTCAAGCGC AAGCAGAGAA ACGAACTTCT GCTTGCAGTC 
AAGAGTCGCC TAGATCCGCC GACCAAGACG CAGCGCGATG TCGTGGAACC CACCACCACC 
ACCGCACCCA CCTACCTGCA GTGCATCCTG ATACCGTGCA AAACGCAAAC GGTTTGGGAG 
CCGCACGTGA CCGCCAGTCG CCTGTTTTTC TGGCGGGAAT TGTGGAACGC CAAGGAACTG 
AAGCGACTGC CCACTTGTCC CGCCGCCCGC GACTGCATCT ACATGTGCTG CAATCCGCTG 
CATTGGTTTC GCATCCTGCA CCAGCCCGAA ACAGAATCCC CAACACCGCC GTACCAACGC 
TCAAAAATGC TGAGACTGAA AGATGCAGAT TTCGAAGAAG ACTCGCAGAA CGATGCAAAG 
TCGGCAGCGC TCAGCACGTG GAGTGCCGAA AGCACCAGCA TTTCGAACAT CTACAAGCCG 
GCTCTCTACG AGTCGGTTAC CACTGATGGA AAAGATCATA ACATCAACAG TCAGGTGTGG 
TGCCAAATCG CCTACTGGGA GATGGCCCAC CGCGTCGGGG AGTTCTTTCA CGCCAAAACG 
AATGCAGTCA ATATCTACAC GGATGGGATT GTGGCCAGTG AGGTGGACAG CATGTGCCTA 
CGTGACCTCA CACCAGCAGG CAATCAGATC CACTCGGTGG TGCCAAAAGC GCGTCATACG 
GTCGGATTGG GGGTCACATT GAGTCTGGAG AACGGCGATG TTTGGATCTA CAATCGTGGG 
AATACAACCA TTTTTGTCGA TTCACCCACC CTGTCCGAAA ATCTGGACCG AGTGTGCAAA 
GTGATGCCGG GCTATTGTCT GAAGGCCTTT GAAACCAATA GGGCTGAACT TCTGAGCATG 
AGAGATCACG GACATCATCC CATGGGACCC GTCGACTACT TCAGCATTAA GATCAGCTTT 
GGCAAGGGCT GGGGACGCGA CTACAAAAGA CAGGATATCA TGGGCTGCCC CTGCTGGCTG 
GAGGTTCACT TTAGTCATCT GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dad cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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