Patr-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Patr-1
Entrez Gene ID	42058
Full Name	Protein associated with topo II related - 1
Synonyms	BcDNA:LD27979,CG5208,Dmel\CG5208,HPat,LD27979,Part-1,anon-WO0118547.282,hpat,l(3)06442,patr-1
General protein information	Preferred Names Protein associated with topo II related - 1 Names CG5208 gene product from transcript CG5208-RB CG5208-PA CG5208-PB Patr-1-PA Patr-1-PB lethal (3) 06442 protein associated with topo II related - 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89E10-89E10

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300440.1	NP_001287369.1	protein associated with topo II related - 1, isoform B
NM_142335.3	NP_650592.1	protein associated with topo II related - 1, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Patr-1	NP_650592.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009631	XP_318663.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HPat couples mRNA deadenylation and decapping in Drosophila.
Title: HPat a decapping activator interacting with the miRNA effector complex.

HPat binds to and copurifies with GW182.
Title: The decapping activator HPat a novel factor co-purifying with GW182 from Drosophila cells.

Results reveal an unprecedented role for the proline-rich region and the C-terminal domain of metazoan HPat in mRNA decapping and suggest that HPat is a component of the cellular mechanism that couples decapping to deadenylation in vivo.
Title: HPat provides a link between deadenylation and decapping in metazoa.

The following Patr-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Patr-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05186	NM_001300440.1 Latest version!	Drosophila melanogaster protein associated with topo II related - 1, transcript variant B (Patr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa05186	NM_142335.3 Latest version!	Drosophila melanogaster protein associated with topo II related - 1, transcript variant A (Patr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Patr-1 ( NM_001300440.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287369.1 Drosophila melanogaster protein associated with topo II related - 1, transcript variant B (Patr-1), mRNA.

Price & Availability↑

CloneID	OFa05186
Clone ID Related Accession (Same CDS sequence)	NM_001300440.1 , NM_142335.3
Accession Version	NM_001300440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein associated with topo II related - 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACT CGTTTTTCGG CTTTGACACG AATCTACCGG ACGAAGATGG TGGCGATGGA 
CGCATCGTCG AGGCGGAATA CGATGCCCTC AATGACGAAA CCTTTGGATC CGCTATAAAT 
GGCGACTGGG AGGAAGCCCA TGAGACGATG GTACGCCTGG GCGGGAATGG CGAAAGGGTG 
CGGAAGCGAC CCGCGGAATC TAGTGGAAAT ACGGATTTTG CCGATCAGGG AAATGGTGCC 
TTCCTGCGAC ACGCTAATCC GCTATCATCG GCGCCCTCTT CAAGCGGCTC CACTCCGGCC 
CCTTTGAATG CCCCCTTCCG ACAGTCTCGT CACATAGGGG ACTCGGACTT GGAGCTAAAC 
ATTTCCTCCA TGAAGTTGGA CGACATGGAC CTGTCCTCTT TCACCACTGA TAGCGAAGCC 
GGTGGGCTGA GCAACCGCAT TAAGCTGGAC TCCGGGGTCT GGGGATCACA GCCGTTTCCC 
AACAACCAAC ATTTGTTCCG CGAGCCTATG CGCAGCGCCT TCAAGCCACA GCAGCAGCAA 
CATCAGCAGC ACCAACCACA ACAAATGAAG CCACCGCAGG AAGCGAATGC CAACTTTGCA 
CTGAATCCAC AGCCTTTTCC AAAAATTACA ACTCTCGAGG ATATAGAGCG CAATATGATC 
ATTCAGCAGG CCATTCCGAA ACAACAGCAG CAGAGCCAGC AACAGGCGGA ACGCAAAATG 
TTTGATGATT TTAGTCTGGG TAACAGGCAG CAGGTGCCTA CACCAACCAT GTTGCAGCAA 
CAGATGTTGC AGCAGCAGCA TCATCAGAAA CAACAGTCGC AGCAGCAGCC GCCACAACAA 
GCGCAGCACA AGGTACCCCC TGGCTTCTTG GGCACGCCGC ACACCTCTCC ACCGAGGCCA 
AATTTGGGTT TCCCCGGACC CGAGCTACTG CCCACGCCAT CGTCGCAGCA ACAACTAAAC 
GGTATTGGTG GCAACCGAGT GCCGCCCGGA TTTATTTACC CGCAAGGTCT GCCCGGGAAT 
ATTCCGCAAT TGCCCCAGCA TCCTCTAATG CAGCAGCACA TGCCGCACAA CTTTCCACTG 
CCCGGCGGCA ACCAGCGACC TCTACCCAAT CCACAAGCTC TGCCCACGGC CCTGAACAAT 
TTCGCGATGC ATCCAAGCTT CAATGCGATG CGCGCCGCTG GAATCCATCC AGGGGCTTTT 
ATGCAACCTC CCCATCCGCA CCAGATGCTG CCACCGCGAA TGCCGCCGCA CCCGCTGCAA 
GCTGCCACAA GTCTATTAGG TCAGCAGCCA CCGAATTCCA TGTATAACAT GTTCAACATG 
CGACTAGTGC AAGAGATCCA GCAAAACCAT CCGCTGCTTC AACAGGCTGC TGTTGTACGC 
CAAATGCAAC AGAGCAGAGC AGGATCCGTG GCCAGCGATC GTCAGAGATC GCAGGCGCAA 
CAGCAGCAAC AAGGACGACG TCCAGATGGT ACAGGAAATT ACCCGCTGGA AGAGTACGAT 
GAATATGCCA ACCTAATGAG CACGCGCGAC AAGCACTGGC TAATCGGAAT CCAGCTTTCG 
CAGCTGAATA CGGACACGCC GTATATCGAC GACTATTACT ACACGGTGTT CAGGGAGCGC 
AAGGCCCAAC AGAACGGGCA AATGCGCAAC AGTCAGGCGC ACAAGGACAA CCAGTTGAAT 
CATCCGCTTA CTCAGCCACG CGGCCATGCG CAGCTTATTC TCGTACAGTT GGGCAACAAG 
AATGGTACTC GCAATGGACA CGGACGTGAG CGACGCAACT CGGAGAACAC CAACAGCACG 
TCAGGCAACA CGAACAATGG ATCAGGTGGT AACAACAACA ACCTGACAGG CTATATATTC 
TCACCACTGA AGTTCGAGAA CTCACTGGGA AAGCTACAGT ACGGAAGTGT GACAGCTCCG 
CGAAAAATTA TCGATGCTGA CATTATGAGT GGTGAATCAA ATGCCGCTGC TGAAGCCAAC 
GCAACTTCTT CATCAAAAAG CTCGACTCCG CTGCCCACCT CCGCGAGCCA TGATGTAAAT 
CCCAGTAGCA TGCGGAAGTC GCGACACATT TTGCTTCTGA TTGAAACACT GTATCGCTAT 
CTGCTGAAGT TGGAGGATTT GGAAAGCCCA GAAGTGATGG CCACCATTGA GCTGAAAAAG 
AAAAAGGAAG CCGAACGGAT CGCAGCCCTT GAGCAGCTGG AAATGGCCAA TAAAACGCCC 
GAAGAACGTG CTGCTGAAGC CGCCAATCCG CAAACCATGA ATCCGCAACT AAAGAACAAG 
TTCAACTATG AGGTAGAGAC GAAAGCTGCG TTGGTCAATA AATTAAAGGC AGGTCTGGCC 
TTCGACAAGG TTATCGCCAT GATGAATGTG CGCAAGGGAA AGATACTTAT TAGACGCATA 
ATGCCCTTTA TTGCGGATCA AAGCATTCGC TGGGTCGTGT GGAGCGGCGT CTTCTGTTCC 
CTACAAACAG TGGTGAAAAA GGACAAGGAC GACGTTGAAG GCGTCTTGTA CGCTCTTTAC 
CCAGAGTTTA AAAAACACGT TAACAAAGCT ACTTTCGAAC TCCTAGTGAG CATTTCATCT 
GCTATTACGC TGAATGACAA GAAACTTGCT GGAATTTTTT GCAGTCGCTT TGGAATCCTT 
TCGCTGGTTG CCCTGATACT TCGCGCCGAG GAAATCTACG TCTCGCGAAC TGACGCAACT 
CTTAGCGAGG AGAACAGGCA AAAGTGGCGA GAATTTCTGG CACAGGTTGC ATCCTGCCTC 
AATCGCACTA TTCAAAACCA GACCATCTCG GCAGCCATTG AGTCGGATGC AATTCAGCCA 
CTTATGGACC ACTTTGAACG GTTTAAAGAT CTTAAACTGG ATGCACTCTT AGCATTAATA 
ACTGAAGCCA GGCATCAAAT TGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287369.1
CDS	87..2993
Translation	MDDSFFGFDTNLPDEDGGDGRIVEAEYDALNDETFGSAINGDWEEAHETMVRLGGNGERVRKRPAESSGNTDFADQGNGAFLRHANPLSSAPSSSGSTPAPLNAPFRQSRHIGDSDLELNISSMKLDDMDLSSFTTDSEAGGLSNRIKLDSGVWGSQPFPNNQHLFREPMRSAFKPQQQQHQQHQPQQMKPPQEANANFALNPQPFPKITTLEDIERNMIIQQAIPKQQQQSQQQAERKMFDDFSLGNRQQVPTPTMLQQQMLQQQHHQKQQSQQQPPQQAQHKVPPGFLGTPHTSPPRPNLGFPGPELLPTPSSQQQLNGIGGNRVPPGFIYPQGLPGNIPQLPQHPLMQQHMPHNFPLPGGNQRPLPNPQALPTALNNFAMHPSFNAMRAAGIHPGAFMQPPHPHQMLPPRMPPHPLQAATSLLGQQPPNSMYNMFNMRLVQEIQQNHPLLQQAAVVRQMQQSRAGSVASDRQRSQAQQQQQGRRPDGTGNYPLEEYDEYANLMSTRDKHWLIGIQLSQLNTDTPYIDDYYYTVFRERKAQQNGQMRNSQAHKDNQLNHPLTQPRGHAQLILVQLGNKNGTRNGHGRERRNSENTNSTSGNTNNGSGGNNNNLTGYIFSPLKFENSLGKLQYGSVTAPRKIIDADIMSGESNAAAEANATSSSKSSTPLPTSASHDVNPSSMRKSRHILLLIETLYRYLLKLEDLESPEVMATIELKKKKEAERIAALEQLEMANKTPEERAAEAANPQTMNPQLKNKFNYEVETKAALVNKLKAGLAFDKVIAMMNVRKGKILIRRIMPFIADQSIRWVVWSGVFCSLQTVVKKDKDDVEGVLYALYPEFKKHVNKATFELLVSISSAITLNDKKLAGIFCSRFGILSLVALILRAEEIYVSRTDATLSEENRQKWREFLAQVASCLNRTIQNQTISAAIESDAIQPLMDHFERFKDLKLDALLALITEARHQID

Target ORF information:

RefSeq Version	NM_001300440.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein associated with topo II related - 1, transcript variant B (Patr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300440.1

ORF Insert Sequence:

ATGGATGACT CGTTTTTCGG CTTTGACACG AATCTACCGG ACGAAGATGG TGGCGATGGA 
CGCATCGTCG AGGCGGAATA CGATGCCCTC AATGACGAAA CCTTTGGATC CGCTATAAAT 
GGCGACTGGG AGGAAGCCCA TGAGACGATG GTACGCCTGG GCGGGAATGG CGAAAGGGTG 
CGGAAGCGAC CCGCGGAATC TAGTGGAAAT ACGGATTTTG CCGATCAGGG AAATGGTGCC 
TTCCTGCGAC ACGCTAATCC GCTATCATCG GCGCCCTCTT CAAGCGGCTC CACTCCGGCC 
CCTTTGAATG CCCCCTTCCG ACAGTCTCGT CACATAGGGG ACTCGGACTT GGAGCTAAAC 
ATTTCCTCCA TGAAGTTGGA CGACATGGAC CTGTCCTCTT TCACCACTGA TAGCGAAGCC 
GGTGGGCTGA GCAACCGCAT TAAGCTGGAC TCCGGGGTCT GGGGATCACA GCCGTTTCCC 
AACAACCAAC ATTTGTTCCG CGAGCCTATG CGCAGCGCCT TCAAGCCACA GCAGCAGCAA 
CATCAGCAGC ACCAACCACA ACAAATGAAG CCACCGCAGG AAGCGAATGC CAACTTTGCA 
CTGAATCCAC AGCCTTTTCC AAAAATTACA ACTCTCGAGG ATATAGAGCG CAATATGATC 
ATTCAGCAGG CCATTCCGAA ACAACAGCAG CAGAGCCAGC AACAGGCGGA ACGCAAAATG 
TTTGATGATT TTAGTCTGGG TAACAGGCAG CAGGTGCCTA CACCAACCAT GTTGCAGCAA 
CAGATGTTGC AGCAGCAGCA TCATCAGAAA CAACAGTCGC AGCAGCAGCC GCCACAACAA 
GCGCAGCACA AGGTACCCCC TGGCTTCTTG GGCACGCCGC ACACCTCTCC ACCGAGGCCA 
AATTTGGGTT TCCCCGGACC CGAGCTACTG CCCACGCCAT CGTCGCAGCA ACAACTAAAC 
GGTATTGGTG GCAACCGAGT GCCGCCCGGA TTTATTTACC CGCAAGGTCT GCCCGGGAAT 
ATTCCGCAAT TGCCCCAGCA TCCTCTAATG CAGCAGCACA TGCCGCACAA CTTTCCACTG 
CCCGGCGGCA ACCAGCGACC TCTACCCAAT CCACAAGCTC TGCCCACGGC CCTGAACAAT 
TTCGCGATGC ATCCAAGCTT CAATGCGATG CGCGCCGCTG GAATCCATCC AGGGGCTTTT 
ATGCAACCTC CCCATCCGCA CCAGATGCTG CCACCGCGAA TGCCGCCGCA CCCGCTGCAA 
GCTGCCACAA GTCTATTAGG TCAGCAGCCA CCGAATTCCA TGTATAACAT GTTCAACATG 
CGACTAGTGC AAGAGATCCA GCAAAACCAT CCGCTGCTTC AACAGGCTGC TGTTGTACGC 
CAAATGCAAC AGAGCAGAGC AGGATCCGTG GCCAGCGATC GTCAGAGATC GCAGGCGCAA 
CAGCAGCAAC AAGGACGACG TCCAGATGGT ACAGGAAATT ACCCGCTGGA AGAGTACGAT 
GAATATGCCA ACCTAATGAG CACGCGCGAC AAGCACTGGC TAATCGGAAT CCAGCTTTCG 
CAGCTGAATA CGGACACGCC GTATATCGAC GACTATTACT ACACGGTGTT CAGGGAGCGC 
AAGGCCCAAC AGAACGGGCA AATGCGCAAC AGTCAGGCGC ACAAGGACAA CCAGTTGAAT 
CATCCGCTTA CTCAGCCACG CGGCCATGCG CAGCTTATTC TCGTACAGTT GGGCAACAAG 
AATGGTACTC GCAATGGACA CGGACGTGAG CGACGCAACT CGGAGAACAC CAACAGCACG 
TCAGGCAACA CGAACAATGG ATCAGGTGGT AACAACAACA ACCTGACAGG CTATATATTC 
TCACCACTGA AGTTCGAGAA CTCACTGGGA AAGCTACAGT ACGGAAGTGT GACAGCTCCG 
CGAAAAATTA TCGATGCTGA CATTATGAGT GGTGAATCAA ATGCCGCTGC TGAAGCCAAC 
GCAACTTCTT CATCAAAAAG CTCGACTCCG CTGCCCACCT CCGCGAGCCA TGATGTAAAT 
CCCAGTAGCA TGCGGAAGTC GCGACACATT TTGCTTCTGA TTGAAACACT GTATCGCTAT 
CTGCTGAAGT TGGAGGATTT GGAAAGCCCA GAAGTGATGG CCACCATTGA GCTGAAAAAG 
AAAAAGGAAG CCGAACGGAT CGCAGCCCTT GAGCAGCTGG AAATGGCCAA TAAAACGCCC 
GAAGAACGTG CTGCTGAAGC CGCCAATCCG CAAACCATGA ATCCGCAACT AAAGAACAAG 
TTCAACTATG AGGTAGAGAC GAAAGCTGCG TTGGTCAATA AATTAAAGGC AGGTCTGGCC 
TTCGACAAGG TTATCGCCAT GATGAATGTG CGCAAGGGAA AGATACTTAT TAGACGCATA 
ATGCCCTTTA TTGCGGATCA AAGCATTCGC TGGGTCGTGT GGAGCGGCGT CTTCTGTTCC 
CTACAAACAG TGGTGAAAAA GGACAAGGAC GACGTTGAAG GCGTCTTGTA CGCTCTTTAC 
CCAGAGTTTA AAAAACACGT TAACAAAGCT ACTTTCGAAC TCCTAGTGAG CATTTCATCT 
GCTATTACGC TGAATGACAA GAAACTTGCT GGAATTTTTT GCAGTCGCTT TGGAATCCTT 
TCGCTGGTTG CCCTGATACT TCGCGCCGAG GAAATCTACG TCTCGCGAAC TGACGCAACT 
CTTAGCGAGG AGAACAGGCA AAAGTGGCGA GAATTTCTGG CACAGGTTGC ATCCTGCCTC 
AATCGCACTA TTCAAAACCA GACCATCTCG GCAGCCATTG AGTCGGATGC AATTCAGCCA 
CTTATGGACC ACTTTGAACG GTTTAAAGAT CTTAAACTGG ATGCACTCTT AGCATTAATA 
ACTGAAGCCA GGCATCAAAT TGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Patr-1 ( NM_142335.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650592.1 Drosophila melanogaster protein associated with topo II related - 1, transcript variant A (Patr-1), mRNA.

Price & Availability↑

CloneID	OFa05186
Clone ID Related Accession (Same CDS sequence)	NM_001300440.1 , NM_142335.3
Accession Version	NM_142335.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein associated with topo II related - 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142335.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACT CGTTTTTCGG CTTTGACACG AATCTACCGG ACGAAGATGG TGGCGATGGA 
CGCATCGTCG AGGCGGAATA CGATGCCCTC AATGACGAAA CCTTTGGATC CGCTATAAAT 
GGCGACTGGG AGGAAGCCCA TGAGACGATG GTACGCCTGG GCGGGAATGG CGAAAGGGTG 
CGGAAGCGAC CCGCGGAATC TAGTGGAAAT ACGGATTTTG CCGATCAGGG AAATGGTGCC 
TTCCTGCGAC ACGCTAATCC GCTATCATCG GCGCCCTCTT CAAGCGGCTC CACTCCGGCC 
CCTTTGAATG CCCCCTTCCG ACAGTCTCGT CACATAGGGG ACTCGGACTT GGAGCTAAAC 
ATTTCCTCCA TGAAGTTGGA CGACATGGAC CTGTCCTCTT TCACCACTGA TAGCGAAGCC 
GGTGGGCTGA GCAACCGCAT TAAGCTGGAC TCCGGGGTCT GGGGATCACA GCCGTTTCCC 
AACAACCAAC ATTTGTTCCG CGAGCCTATG CGCAGCGCCT TCAAGCCACA GCAGCAGCAA 
CATCAGCAGC ACCAACCACA ACAAATGAAG CCACCGCAGG AAGCGAATGC CAACTTTGCA 
CTGAATCCAC AGCCTTTTCC AAAAATTACA ACTCTCGAGG ATATAGAGCG CAATATGATC 
ATTCAGCAGG CCATTCCGAA ACAACAGCAG CAGAGCCAGC AACAGGCGGA ACGCAAAATG 
TTTGATGATT TTAGTCTGGG TAACAGGCAG CAGGTGCCTA CACCAACCAT GTTGCAGCAA 
CAGATGTTGC AGCAGCAGCA TCATCAGAAA CAACAGTCGC AGCAGCAGCC GCCACAACAA 
GCGCAGCACA AGGTACCCCC TGGCTTCTTG GGCACGCCGC ACACCTCTCC ACCGAGGCCA 
AATTTGGGTT TCCCCGGACC CGAGCTACTG CCCACGCCAT CGTCGCAGCA ACAACTAAAC 
GGTATTGGTG GCAACCGAGT GCCGCCCGGA TTTATTTACC CGCAAGGTCT GCCCGGGAAT 
ATTCCGCAAT TGCCCCAGCA TCCTCTAATG CAGCAGCACA TGCCGCACAA CTTTCCACTG 
CCCGGCGGCA ACCAGCGACC TCTACCCAAT CCACAAGCTC TGCCCACGGC CCTGAACAAT 
TTCGCGATGC ATCCAAGCTT CAATGCGATG CGCGCCGCTG GAATCCATCC AGGGGCTTTT 
ATGCAACCTC CCCATCCGCA CCAGATGCTG CCACCGCGAA TGCCGCCGCA CCCGCTGCAA 
GCTGCCACAA GTCTATTAGG TCAGCAGCCA CCGAATTCCA TGTATAACAT GTTCAACATG 
CGACTAGTGC AAGAGATCCA GCAAAACCAT CCGCTGCTTC AACAGGCTGC TGTTGTACGC 
CAAATGCAAC AGAGCAGAGC AGGATCCGTG GCCAGCGATC GTCAGAGATC GCAGGCGCAA 
CAGCAGCAAC AAGGACGACG TCCAGATGGT ACAGGAAATT ACCCGCTGGA AGAGTACGAT 
GAATATGCCA ACCTAATGAG CACGCGCGAC AAGCACTGGC TAATCGGAAT CCAGCTTTCG 
CAGCTGAATA CGGACACGCC GTATATCGAC GACTATTACT ACACGGTGTT CAGGGAGCGC 
AAGGCCCAAC AGAACGGGCA AATGCGCAAC AGTCAGGCGC ACAAGGACAA CCAGTTGAAT 
CATCCGCTTA CTCAGCCACG CGGCCATGCG CAGCTTATTC TCGTACAGTT GGGCAACAAG 
AATGGTACTC GCAATGGACA CGGACGTGAG CGACGCAACT CGGAGAACAC CAACAGCACG 
TCAGGCAACA CGAACAATGG ATCAGGTGGT AACAACAACA ACCTGACAGG CTATATATTC 
TCACCACTGA AGTTCGAGAA CTCACTGGGA AAGCTACAGT ACGGAAGTGT GACAGCTCCG 
CGAAAAATTA TCGATGCTGA CATTATGAGT GGTGAATCAA ATGCCGCTGC TGAAGCCAAC 
GCAACTTCTT CATCAAAAAG CTCGACTCCG CTGCCCACCT CCGCGAGCCA TGATGTAAAT 
CCCAGTAGCA TGCGGAAGTC GCGACACATT TTGCTTCTGA TTGAAACACT GTATCGCTAT 
CTGCTGAAGT TGGAGGATTT GGAAAGCCCA GAAGTGATGG CCACCATTGA GCTGAAAAAG 
AAAAAGGAAG CCGAACGGAT CGCAGCCCTT GAGCAGCTGG AAATGGCCAA TAAAACGCCC 
GAAGAACGTG CTGCTGAAGC CGCCAATCCG CAAACCATGA ATCCGCAACT AAAGAACAAG 
TTCAACTATG AGGTAGAGAC GAAAGCTGCG TTGGTCAATA AATTAAAGGC AGGTCTGGCC 
TTCGACAAGG TTATCGCCAT GATGAATGTG CGCAAGGGAA AGATACTTAT TAGACGCATA 
ATGCCCTTTA TTGCGGATCA AAGCATTCGC TGGGTCGTGT GGAGCGGCGT CTTCTGTTCC 
CTACAAACAG TGGTGAAAAA GGACAAGGAC GACGTTGAAG GCGTCTTGTA CGCTCTTTAC 
CCAGAGTTTA AAAAACACGT TAACAAAGCT ACTTTCGAAC TCCTAGTGAG CATTTCATCT 
GCTATTACGC TGAATGACAA GAAACTTGCT GGAATTTTTT GCAGTCGCTT TGGAATCCTT 
TCGCTGGTTG CCCTGATACT TCGCGCCGAG GAAATCTACG TCTCGCGAAC TGACGCAACT 
CTTAGCGAGG AGAACAGGCA AAAGTGGCGA GAATTTCTGG CACAGGTTGC ATCCTGCCTC 
AATCGCACTA TTCAAAACCA GACCATCTCG GCAGCCATTG AGTCGGATGC AATTCAGCCA 
CTTATGGACC ACTTTGAACG GTTTAAAGAT CTTAAACTGG ATGCACTCTT AGCATTAATA 
ACTGAAGCCA GGCATCAAAT TGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650592.1
CDS	87..2993
Translation	MDDSFFGFDTNLPDEDGGDGRIVEAEYDALNDETFGSAINGDWEEAHETMVRLGGNGERVRKRPAESSGNTDFADQGNGAFLRHANPLSSAPSSSGSTPAPLNAPFRQSRHIGDSDLELNISSMKLDDMDLSSFTTDSEAGGLSNRIKLDSGVWGSQPFPNNQHLFREPMRSAFKPQQQQHQQHQPQQMKPPQEANANFALNPQPFPKITTLEDIERNMIIQQAIPKQQQQSQQQAERKMFDDFSLGNRQQVPTPTMLQQQMLQQQHHQKQQSQQQPPQQAQHKVPPGFLGTPHTSPPRPNLGFPGPELLPTPSSQQQLNGIGGNRVPPGFIYPQGLPGNIPQLPQHPLMQQHMPHNFPLPGGNQRPLPNPQALPTALNNFAMHPSFNAMRAAGIHPGAFMQPPHPHQMLPPRMPPHPLQAATSLLGQQPPNSMYNMFNMRLVQEIQQNHPLLQQAAVVRQMQQSRAGSVASDRQRSQAQQQQQGRRPDGTGNYPLEEYDEYANLMSTRDKHWLIGIQLSQLNTDTPYIDDYYYTVFRERKAQQNGQMRNSQAHKDNQLNHPLTQPRGHAQLILVQLGNKNGTRNGHGRERRNSENTNSTSGNTNNGSGGNNNNLTGYIFSPLKFENSLGKLQYGSVTAPRKIIDADIMSGESNAAAEANATSSSKSSTPLPTSASHDVNPSSMRKSRHILLLIETLYRYLLKLEDLESPEVMATIELKKKKEAERIAALEQLEMANKTPEERAAEAANPQTMNPQLKNKFNYEVETKAALVNKLKAGLAFDKVIAMMNVRKGKILIRRIMPFIADQSIRWVVWSGVFCSLQTVVKKDKDDVEGVLYALYPEFKKHVNKATFELLVSISSAITLNDKKLAGIFCSRFGILSLVALILRAEEIYVSRTDATLSEENRQKWREFLAQVASCLNRTIQNQTISAAIESDAIQPLMDHFERFKDLKLDALLALITEARHQID

Target ORF information:

RefSeq Version	NM_142335.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein associated with topo II related - 1, transcript variant A (Patr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142335.3

ORF Insert Sequence:

ATGGATGACT CGTTTTTCGG CTTTGACACG AATCTACCGG ACGAAGATGG TGGCGATGGA 
CGCATCGTCG AGGCGGAATA CGATGCCCTC AATGACGAAA CCTTTGGATC CGCTATAAAT 
GGCGACTGGG AGGAAGCCCA TGAGACGATG GTACGCCTGG GCGGGAATGG CGAAAGGGTG 
CGGAAGCGAC CCGCGGAATC TAGTGGAAAT ACGGATTTTG CCGATCAGGG AAATGGTGCC 
TTCCTGCGAC ACGCTAATCC GCTATCATCG GCGCCCTCTT CAAGCGGCTC CACTCCGGCC 
CCTTTGAATG CCCCCTTCCG ACAGTCTCGT CACATAGGGG ACTCGGACTT GGAGCTAAAC 
ATTTCCTCCA TGAAGTTGGA CGACATGGAC CTGTCCTCTT TCACCACTGA TAGCGAAGCC 
GGTGGGCTGA GCAACCGCAT TAAGCTGGAC TCCGGGGTCT GGGGATCACA GCCGTTTCCC 
AACAACCAAC ATTTGTTCCG CGAGCCTATG CGCAGCGCCT TCAAGCCACA GCAGCAGCAA 
CATCAGCAGC ACCAACCACA ACAAATGAAG CCACCGCAGG AAGCGAATGC CAACTTTGCA 
CTGAATCCAC AGCCTTTTCC AAAAATTACA ACTCTCGAGG ATATAGAGCG CAATATGATC 
ATTCAGCAGG CCATTCCGAA ACAACAGCAG CAGAGCCAGC AACAGGCGGA ACGCAAAATG 
TTTGATGATT TTAGTCTGGG TAACAGGCAG CAGGTGCCTA CACCAACCAT GTTGCAGCAA 
CAGATGTTGC AGCAGCAGCA TCATCAGAAA CAACAGTCGC AGCAGCAGCC GCCACAACAA 
GCGCAGCACA AGGTACCCCC TGGCTTCTTG GGCACGCCGC ACACCTCTCC ACCGAGGCCA 
AATTTGGGTT TCCCCGGACC CGAGCTACTG CCCACGCCAT CGTCGCAGCA ACAACTAAAC 
GGTATTGGTG GCAACCGAGT GCCGCCCGGA TTTATTTACC CGCAAGGTCT GCCCGGGAAT 
ATTCCGCAAT TGCCCCAGCA TCCTCTAATG CAGCAGCACA TGCCGCACAA CTTTCCACTG 
CCCGGCGGCA ACCAGCGACC TCTACCCAAT CCACAAGCTC TGCCCACGGC CCTGAACAAT 
TTCGCGATGC ATCCAAGCTT CAATGCGATG CGCGCCGCTG GAATCCATCC AGGGGCTTTT 
ATGCAACCTC CCCATCCGCA CCAGATGCTG CCACCGCGAA TGCCGCCGCA CCCGCTGCAA 
GCTGCCACAA GTCTATTAGG TCAGCAGCCA CCGAATTCCA TGTATAACAT GTTCAACATG 
CGACTAGTGC AAGAGATCCA GCAAAACCAT CCGCTGCTTC AACAGGCTGC TGTTGTACGC 
CAAATGCAAC AGAGCAGAGC AGGATCCGTG GCCAGCGATC GTCAGAGATC GCAGGCGCAA 
CAGCAGCAAC AAGGACGACG TCCAGATGGT ACAGGAAATT ACCCGCTGGA AGAGTACGAT 
GAATATGCCA ACCTAATGAG CACGCGCGAC AAGCACTGGC TAATCGGAAT CCAGCTTTCG 
CAGCTGAATA CGGACACGCC GTATATCGAC GACTATTACT ACACGGTGTT CAGGGAGCGC 
AAGGCCCAAC AGAACGGGCA AATGCGCAAC AGTCAGGCGC ACAAGGACAA CCAGTTGAAT 
CATCCGCTTA CTCAGCCACG CGGCCATGCG CAGCTTATTC TCGTACAGTT GGGCAACAAG 
AATGGTACTC GCAATGGACA CGGACGTGAG CGACGCAACT CGGAGAACAC CAACAGCACG 
TCAGGCAACA CGAACAATGG ATCAGGTGGT AACAACAACA ACCTGACAGG CTATATATTC 
TCACCACTGA AGTTCGAGAA CTCACTGGGA AAGCTACAGT ACGGAAGTGT GACAGCTCCG 
CGAAAAATTA TCGATGCTGA CATTATGAGT GGTGAATCAA ATGCCGCTGC TGAAGCCAAC 
GCAACTTCTT CATCAAAAAG CTCGACTCCG CTGCCCACCT CCGCGAGCCA TGATGTAAAT 
CCCAGTAGCA TGCGGAAGTC GCGACACATT TTGCTTCTGA TTGAAACACT GTATCGCTAT 
CTGCTGAAGT TGGAGGATTT GGAAAGCCCA GAAGTGATGG CCACCATTGA GCTGAAAAAG 
AAAAAGGAAG CCGAACGGAT CGCAGCCCTT GAGCAGCTGG AAATGGCCAA TAAAACGCCC 
GAAGAACGTG CTGCTGAAGC CGCCAATCCG CAAACCATGA ATCCGCAACT AAAGAACAAG 
TTCAACTATG AGGTAGAGAC GAAAGCTGCG TTGGTCAATA AATTAAAGGC AGGTCTGGCC 
TTCGACAAGG TTATCGCCAT GATGAATGTG CGCAAGGGAA AGATACTTAT TAGACGCATA 
ATGCCCTTTA TTGCGGATCA AAGCATTCGC TGGGTCGTGT GGAGCGGCGT CTTCTGTTCC 
CTACAAACAG TGGTGAAAAA GGACAAGGAC GACGTTGAAG GCGTCTTGTA CGCTCTTTAC 
CCAGAGTTTA AAAAACACGT TAACAAAGCT ACTTTCGAAC TCCTAGTGAG CATTTCATCT 
GCTATTACGC TGAATGACAA GAAACTTGCT GGAATTTTTT GCAGTCGCTT TGGAATCCTT 
TCGCTGGTTG CCCTGATACT TCGCGCCGAG GAAATCTACG TCTCGCGAAC TGACGCAACT 
CTTAGCGAGG AGAACAGGCA AAAGTGGCGA GAATTTCTGG CACAGGTTGC ATCCTGCCTC 
AATCGCACTA TTCAAAACCA GACCATCTCG GCAGCCATTG AGTCGGATGC AATTCAGCCA 
CTTATGGACC ACTTTGAACG GTTTAAAGAT CTTAAACTGG ATGCACTCTT AGCATTAATA 
ACTGAAGCCA GGCATCAAAT TGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Patr-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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