Gyc89Db cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gyc89Db
Entrez Gene ID	42002
Full Name	Guanylyl cyclase at 89Db
Synonyms	CG14886,Dmel\CG14886,Gyc-89Db
General protein information	Preferred Names Guanylyl cyclase at 89Db Names CG14886 gene product from transcript CG14886-RA CG14886-PA Gyc89Db-PA guanylyl cyclase at 89Db
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89B18-89B18

mRNA and Protein(s)

mRNA	Protein	Name
NM_142294.4	NP_650551.1	guanylyl cyclase at 89Db

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004564	XP_313866.5
Drosophila melanogaster (fruit fly)	Gyc-89Db	NP_650551.1
Drosophila melanogaster (fruit fly)	Gyc-89Da	NP_001036718.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gyc-88E and Gyc-89Db are co-expressed in a subset of sensory neurons where they would be ideally situated to act as oxygen sensors
Title: Atypical soluble guanylyl cyclases in Drosophila can function as molecular oxygen sensors.

The following Gyc89Db gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gyc89Db cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05131	NM_142294.4 Latest version!	Drosophila melanogaster guanylyl cyclase at 89Db (Gyc89Db), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gyc89Db ( NM_142294.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650551.1 Drosophila melanogaster guanylyl cyclase at 89Db (Gyc89Db), mRNA.

Price & Availability↑

CloneID	OFa05131
Clone ID Related Accession (Same CDS sequence)	NM_142294.4
Accession Version	NM_142294.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 89Db
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142294.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACGGAA TGCTGTACGA GAGCGTGCAG CACTATATCC AGCAGGAGTA TGGCATGGAG 
ACCTGGCGAA AGGTCTGCCA GATTGTCGAC TGCAAGCATC AGAGCTTCAA GACACATCAG 
ATCTATCCGG ACAAGCTGAT GCCGGACTTT GCGGCAGCCC TGTCCGCCAG CACGGGCGAG 
TCCTTCGACT TCTGCATGAA CTTCTTCGGG CGCTGTTTTG TGCGATTCTT CAGCAACTTC 
GGCTACGACA AGATGATCCG CTCCACAGGG CGATACTTCT GCGACTTCCT GCAGTCCATC 
GACAACATTC ACGTGCAGAT GCGTTTCACC TATCCCAAGA TGAAGTCGCC CAGCATGCAG 
TTGACCAACA TGGATGACGA TGGGGCTGTG ATCCTGTACC GCAGCGGCCG AACTGGCATG 
TCCAAGTACC TGATTGGCCA GATGACCGAG GTGGCCAAGG AGTTCTACGG CCTAGACATG 
ACTGCCTATG TCCTGGAGAG CCAAAATGAC ATATGCGGTG GCACAGCCGG TCCCATCAAG 
CTTACGGAGG GTCCTCTGAC CGTGATTGTC AAGTATCGGC TGGACTTCGA TAATCGCGAT 
TATATGGCCA AGCGGGTCAA TGTCATCGCG CATCCTTCGC AGCTGAAGAT GCCGTCGGTG 
GACCTGAATG TGTTCCTCGA GCTGTTTCCC TTCACCATTG TGCTGGATCA CGACATGAAA 
ATCACACTTG CGGGCGAGAA GATTGTCGAG ACGTGGATTC TGCATAATCC TGGAGTGAAC 
CCAAAGACCT TTATTGGCAG TCACATATTG GAGCGGTTCA AGTGCCGTCG TCCCAAGGAC 
ACTCAGATCC AATGGGAGAC CATTCTGCAA ATGCGTACAG TTCTCTTCGA GTTTGAGCTC 
ATCCGTACGG GTCACAATCG TGCCGCCTAT GATGCGGCCT TGAACTTTGA CTTTGAAAAC 
TTTGATGAAG CCAGCAGCCT GAACGAGGCG CAGGCCATGG CTTTGGCCAG TGCCAAGGAG 
TTTAGTGCGG AGAACGCCAA GGAAGAGGCA GCAGCGGCAG CCACCTCCAA GGACGAAATC 
GATCCCGCCA CGGGACAGAG ACGCCATTCG GTGGGCCTCC GATCTATCCT GTTGAAGGGA 
CAAATGTTCT ATATCAAAGA TGTGGACTCG CTGATCTTCC TGTGCAGTCC TCTAATCGAA 
AATCTCGATG AGCTGCATGG AATCGGTCTC TATTTGAACG ATCTTAATCC GCATGGACTG 
AGTAGAGAGC TTGTGATGGC GGGATGGCAG CACTGCTCCA AGCTGGAGAT AATGTTTGAG 
AAGGAGGAGC AGCGATCGGA TGAGCTGGAA AAGTCGCTGG AACTGGCAGA TTCCTGGAAG 
CGGCAGGGAG ATGAGCTACT GTATTCCATG ATACCGCGAC CCATTGCCGA GCGAATGAGA 
AAGAGCGAGG AGCACGTGTG TCAGAGTTTC GAGGAGGTAT CCGTCATTTT CATCGAGGTA 
ATGAATATCT ATGACAGTGG GTCCAACAAC ATCCAGGATG CCATGCAGGC GGTGACCACC 
TTAAACAAAG TGTTTTCGGC ATTGGACGAA GAGATCATTT CCCCGTTTGT TTACAAAGTG 
GAGACCGTGG GCATGGTTTA CATGGCTGTT TCGGGTGCTC CTGATGTGAA TCCCCTGCAT 
GCCGAACACG CCTGCGATTT GGCCTTAAGG GTGATGAAGA AGGTCAAAGC CCATGCTCTT 
CCCGGGGTGG CTATACGGGT GGGCATTAAC TCGGGACCCG TCGTCGCCGG CGTGGTTGGG 
ATGAAGGTTC CTCGATACTG TCTCTTTGGA GACACCGTTA ACACCGCCTC GCGAATGGAG 
AGCAGCAGCG ATCCCTGGAT GATCCAGCTG TCCAACTATA CTGCGCTGAA GGTGCAAAAG 
GTGGGCTATA AGGTTGAAGC GCGGGGATTT GTCAAGGTCA AGGGCAAGGG CGAGATGGAG 
ACCTACTGGC TTTTGGAGGG ACCAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650551.1
CDS	541..2550
Translation	MYGMLYESVQHYIQQEYGMETWRKVCQIVDCKHQSFKTHQIYPDKLMPDFAAALSASTGESFDFCMNFFGRCFVRFFSNFGYDKMIRSTGRYFCDFLQSIDNIHVQMRFTYPKMKSPSMQLTNMDDDGAVILYRSGRTGMSKYLIGQMTEVAKEFYGLDMTAYVLESQNDICGGTAGPIKLTEGPLTVIVKYRLDFDNRDYMAKRVNVIAHPSQLKMPSVDLNVFLELFPFTIVLDHDMKITLAGEKIVETWILHNPGVNPKTFIGSHILERFKCRRPKDTQIQWETILQMRTVLFEFELIRTGHNRAAYDAALNFDFENFDEASSLNEAQAMALASAKEFSAENAKEEAAAAATSKDEIDPATGQRRHSVGLRSILLKGQMFYIKDVDSLIFLCSPLIENLDELHGIGLYLNDLNPHGLSRELVMAGWQHCSKLEIMFEKEEQRSDELEKSLELADSWKRQGDELLYSMIPRPIAERMRKSEEHVCQSFEEVSVIFIEVMNIYDSGSNNIQDAMQAVTTLNKVFSALDEEIISPFVYKVETVGMVYMAVSGAPDVNPLHAEHACDLALRVMKKVKAHALPGVAIRVGINSGPVVAGVVGMKVPRYCLFGDTVNTASRMESSSDPWMIQLSNYTALKVQKVGYKVEARGFVKVKGKGEMETYWLLEGPE

Target ORF information:

RefSeq Version	NM_142294.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 89Db (Gyc89Db), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142294.4

ORF Insert Sequence:

ATGTACGGAA TGCTGTACGA GAGCGTGCAG CACTATATCC AGCAGGAGTA TGGCATGGAG 
ACCTGGCGAA AGGTCTGCCA GATTGTCGAC TGCAAGCATC AGAGCTTCAA GACACATCAG 
ATCTATCCGG ACAAGCTGAT GCCGGACTTT GCGGCAGCCC TGTCCGCCAG CACGGGCGAG 
TCCTTCGACT TCTGCATGAA CTTCTTCGGG CGCTGTTTTG TGCGATTCTT CAGCAACTTC 
GGCTACGACA AGATGATCCG CTCCACAGGG CGATACTTCT GCGACTTCCT GCAGTCCATC 
GACAACATTC ACGTGCAGAT GCGTTTCACC TATCCCAAGA TGAAGTCGCC CAGCATGCAG 
TTGACCAACA TGGATGACGA TGGGGCTGTG ATCCTGTACC GCAGCGGCCG AACTGGCATG 
TCCAAGTACC TGATTGGCCA GATGACCGAG GTGGCCAAGG AGTTCTACGG CCTAGACATG 
ACTGCCTATG TCCTGGAGAG CCAAAATGAC ATATGCGGTG GCACAGCCGG TCCCATCAAG 
CTTACGGAGG GTCCTCTGAC CGTGATTGTC AAGTATCGGC TGGACTTCGA TAATCGCGAT 
TATATGGCCA AGCGGGTCAA TGTCATCGCG CATCCTTCGC AGCTGAAGAT GCCGTCGGTG 
GACCTGAATG TGTTCCTCGA GCTGTTTCCC TTCACCATTG TGCTGGATCA CGACATGAAA 
ATCACACTTG CGGGCGAGAA GATTGTCGAG ACGTGGATTC TGCATAATCC TGGAGTGAAC 
CCAAAGACCT TTATTGGCAG TCACATATTG GAGCGGTTCA AGTGCCGTCG TCCCAAGGAC 
ACTCAGATCC AATGGGAGAC CATTCTGCAA ATGCGTACAG TTCTCTTCGA GTTTGAGCTC 
ATCCGTACGG GTCACAATCG TGCCGCCTAT GATGCGGCCT TGAACTTTGA CTTTGAAAAC 
TTTGATGAAG CCAGCAGCCT GAACGAGGCG CAGGCCATGG CTTTGGCCAG TGCCAAGGAG 
TTTAGTGCGG AGAACGCCAA GGAAGAGGCA GCAGCGGCAG CCACCTCCAA GGACGAAATC 
GATCCCGCCA CGGGACAGAG ACGCCATTCG GTGGGCCTCC GATCTATCCT GTTGAAGGGA 
CAAATGTTCT ATATCAAAGA TGTGGACTCG CTGATCTTCC TGTGCAGTCC TCTAATCGAA 
AATCTCGATG AGCTGCATGG AATCGGTCTC TATTTGAACG ATCTTAATCC GCATGGACTG 
AGTAGAGAGC TTGTGATGGC GGGATGGCAG CACTGCTCCA AGCTGGAGAT AATGTTTGAG 
AAGGAGGAGC AGCGATCGGA TGAGCTGGAA AAGTCGCTGG AACTGGCAGA TTCCTGGAAG 
CGGCAGGGAG ATGAGCTACT GTATTCCATG ATACCGCGAC CCATTGCCGA GCGAATGAGA 
AAGAGCGAGG AGCACGTGTG TCAGAGTTTC GAGGAGGTAT CCGTCATTTT CATCGAGGTA 
ATGAATATCT ATGACAGTGG GTCCAACAAC ATCCAGGATG CCATGCAGGC GGTGACCACC 
TTAAACAAAG TGTTTTCGGC ATTGGACGAA GAGATCATTT CCCCGTTTGT TTACAAAGTG 
GAGACCGTGG GCATGGTTTA CATGGCTGTT TCGGGTGCTC CTGATGTGAA TCCCCTGCAT 
GCCGAACACG CCTGCGATTT GGCCTTAAGG GTGATGAAGA AGGTCAAAGC CCATGCTCTT 
CCCGGGGTGG CTATACGGGT GGGCATTAAC TCGGGACCCG TCGTCGCCGG CGTGGTTGGG 
ATGAAGGTTC CTCGATACTG TCTCTTTGGA GACACCGTTA ACACCGCCTC GCGAATGGAG 
AGCAGCAGCG ATCCCTGGAT GATCCAGCTG TCCAACTATA CTGCGCTGAA GGTGCAAAAG 
GTGGGCTATA AGGTTGAAGC GCGGGGATTT GTCAAGGTCA AGGGCAAGGG CGAGATGGAG 
ACCTACTGGC TTTTGGAGGG ACCAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gyc89Db cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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