ema cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ema
Entrez Gene ID	41992
Full Name	endosomal maturation defective
Synonyms	CG12753,Dmel\CG12753,Ema
General protein information	Preferred Names endosomal maturation defective Names CG12753 gene product from transcript CG12753-RC CG12753-PA CG12753-PB CG12753-PC ema-PA ema-PB ema-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89B16-89B16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001038969.2	NP_001034058.1	endosomal maturation defective, isoform B
NM_142285.2	NP_650542.1	endosomal maturation defective, isoform A
NM_001275706.1	NP_001262635.1	endosomal maturation defective, isoform C

Pathways from BioSystems

Homologies

	CLEC16A	XP_001104364.2
Rattus norvegicus (Norway rat)	Clec16a	XP_006245804.1
Gallus gallus (chicken)	CLEC16A	XP_414931.3
Caenorhabditis elegans (roundworm)	gop-1	NP_498117.3
Homo sapiens (human)	CLEC16A	NP_056041.1
Pan troglodytes (chimpanzee)	CLEC16A	XP_001141299.1
Bos taurus (cattle)	CLEC16A	XP_002697939.1
Drosophila melanogaster (fruit fly)	ema	NP_650542.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002781	XP_312142.5
Xenopus tropicalis (tropical clawed frog)	clec16a	XP_004918004.1
Canis lupus familiaris (dog)	CLEC16A	XP_003434855.1
Danio rerio (zebrafish)	clec16a	XP_003198156.1
Mus musculus (house mouse)	Clec16a	NP_808230.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study not only identifies a role for Ema in autophagy, but also supports the hypothesis that the Golgi complex may be a potential membrane source for the biogenesis and development of autophagic structures.
Title: A role for the membrane Golgi protein Ema in autophagy.

In fat body cells, Ema localizes to the Golgi complex and is recruited to the membrane of autophagosomes in response to starvation.
Title: Drosophila Golgi membrane protein Ema promotes autophagosomal growth and function.

The following ema gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ema cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05122	NM_001038969.2 Latest version!	Drosophila melanogaster endosomal maturation defective (ema), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa05123	NM_142285.2 Latest version!	Drosophila melanogaster endosomal maturation defective (ema), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa07929	NM_001275706.1 Latest version!	Drosophila melanogaster endosomal maturation defective (ema), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ema ( NM_001038969.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001034058.1 Drosophila melanogaster endosomal maturation defective (ema), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05122
Clone ID Related Accession (Same CDS sequence)	NM_001038969.2
Accession Version	NM_001038969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	endosomal maturation defective, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001038969.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCGGA GTCGCAGCTG GTTTGGCGGA CCCTGGGGTG GGCGGCCCAA AAACCGCTTG 
TCGCTGGAGC ACCTTAAGTA CCTGTACAGC ATTCTGGAGA AGAACACCAC CGTGTCGGAA 
AGTAATCGTG GCCTGCTGGT GGAGTCGCTG CGCTGCATCG CAGAGATCCT GATTTGGGGC 
GACCAGCATG ACTCGCTGGT GTTTGACTTC TTCTTGGAGA AGAATATGCT ATCGTATTTC 
CTGCACATAA TGCGACAAAA GAGTGGAGGC TCGAGTTTCG TCTGCGTGCA GCTCCTTCAG 
ACTCTCAACA TCCTCTTTGA GAACATACGC AACGAGACGT CCCTGTATTA TCTGTTGAGC 
AACAACCATG TAAATTCTAT CATGGTGCAC AAGTTTGACT TCTCCGACGA GGATGTGATG 
GGCTATTATA TACTTTTTTT AAAGACGCTA AGTCTAAAGT TAAACACTCA CACAATACAC 
TTCTTTTACA ATGAGCACAC AAATGACTTT CCCTTGTATA CAGAGGCTAT TAAGTTCTTT 
AATCATCCCG AGTCAATGGT GAGAATTGCA GTGCGCACCA TTTCCTTAAA TGTCTACAAG 
GTTCAAAATC CAAGCATGTT GCGTTTTATT CGAGACAAAA CGGCTGCTCC ATATTTCAGT 
AATCTGGTCT GGTTTATCGG CAAACACATT CTTGAACTAG ACACTTGTGT TCGCACAGAT 
ATAGACCATC AATCGAATCA AAAGCTGTCC AATTTGGTTG CCGAGCATCT GGATCATTTG 
CATTATTTGA GTGACATTCT TCTACTGGAA ATCAAGGATC TGAACGCGGT GTTAACCGAG 
CACTTGCTGC ATAAGCTATT CGTGCCTCTG TACATATTCT CACTTACACC AGCTCCGCCC 
CCGCCCTCAC TGGCAGTGGT TACCCAGAAT CTAGCTGCTG TGCTTAATCG CAATGTGGAC 
ATCGACATAC AGGAGATGCA CAATCCGCGA GTGAGCTCCA TCGTGGCCCT TTTCCTGCTC 
TCATTGGTCT TCCTGGTGGT TTCGCATGCC CCTCTGGTGC ACGCTCTGGC CTGGGTTATC 
CTCAATGGTG ACCACAGCGT TTTTAAGGAG GGTGCAGCCG AGATTCTTAA TTCATACGTT 
GAGCACCGCG AGGTGGTTGT GCCTGGCTTC GGCGAGCCCG ACGAGAGTCT CGAACAGGCC 
TTGGATACAG TCACCGGTCA GTCCAGCTCG TCCAGCTATG CCCTTAGCGA GGACAGTGGC 
GTGGAGTCCT CATCCCCAGC CACCACAGAA CTGGACTCTC AAGCAGATGC GGTTGAAGCG 
GAGCAGATTA AACTGCGCAA CATAACTGAC GAGGAAAAGC AGCTGCTGCA GAAGTCATCT 
TCCTCAACGA AGGCTGACTT TGCTGAGATG GCCAAACCGT TTTTAGACAC GGTCCTGCAT 
GCTTTGGACT GCACCGAAAA CGATTACCTT GCCCTGCTGT CGCTGTGCCT CATATATGCT 
ATGTCGCACA ATAGAGGCAT CAAGAATGAG TGGTTCGAGC AGGTTCTTGC AAAATCAACC 
CGTGGCGCCT TCAGTTATAA GACAGCCTTG ATTGAGCATC TGTTAAACAT CATCACCCAG 
TCCAGTCAGC CATCGTCCCG CATTCGGTTG ATTACCGTGG AGATTGCCTT GGAGTTACTG 
GTCACTTTCA CCCGTCCCAG CTCGGATGAT TCGCGCTGCA TAACCGCCGC ACAACAGGAT 
CTGCTCTTCA GCGCCCGCAA TCAGTCGATG GTGGTGATCC GTAACTTCTA CAAGTCGGAG 
GACATCTTCC TAGACCTGTT CGAAGACGAA TACAACGAGA TGCGAAAGGC GCAACTGAAC 
GTGGAATTCC TGTGCATGGA CTCCACCATT CTACTGCCTC CCACGGGAAC GCCACTTACT 
GGAATCAACT TTACGCGACG ACTTCCCTGC GGGGAGGTGG AAAAGGCACG CCGAGCCATT 
CGCGTTTACT TCCTGCTGCG TCGCACGTGC CAAAAGTTTT TGAATGAGAA GGAATCGCTA 
TTGCCGCTTA CAAATGTAGT CAATCTGGTT CAGGTGGAGA ACGTACTCGA TCTGAACAAT 
AGTGATCTAA TAGCCTGCAC CGTTGTGGCC AAGGACAGCA GCAAGCAAAG ACGCTTTCTG 
GTCATTGACG CATTGCAGCT AATTCTCGTC GAGCCGGATG CAAAGCTGCT TGGCTGGGGC 
GTGGCAAAGT TCGTTGGCTT TCTGCAGGAC GTAGAGGTGC AGGGTGATAA GGATGACTCG 
CGCTGCTTGC ACATCACCGT GCACCGGGGC GGAGTCACTC ACAACCGCAC TCCGTTGCTC 
TCGGCTAAGT TCCTGTTCGA CGATCACATC CGTTGCATGG CTGCCAAGCA GAGGTTGACA 
AAGGGTCGTA GCAAGGCGCG ACAAAAGAAG ATGTACCAGA TTGCGCAACT CATCGAGATT 
CCTGGACAGA TGGACTCTCC CGTCTACGCT GTTGGTGGCA CAATGGTCGC ATCCAGTAGC 
GGCGGAAGTG GTAACAGCAG TGGCAGCAGT TCGCGCAGTA GTCACCACCG TCCCATGTTC 
TCCACGGCCA ATCGAGTGCC GGGATTTGCA GCGGTGCTGC GTGGAAGCAA TAGCGCTGGG 
GTTAGTCGAA CCCAGATGGC CCCGAATCGA TCCATCGAAG GGTCGGAGGA GATTCCTCTC 
GAGGACTTCC AGCACTCACG GAACAACAGT CCGCACTCTC GGGGAAACCC CTCGCCTGCC 
TCCCGCTCAC ACACACCCAT CCGCGTGCTC CACTACGATC AGCTGTCCGG TCATAGCGGC 
TCACCACGTG AAGCGTCCCT GGGGGGAACT AACGCCCTGC TAAGCCAGTT AAATGGGCTG 
AACCGTGAAG TGCTCCCAAC GCAGAGCTCC GAGGAGACTT CTTTTATTGG GAGCGACGGA 
AACGAGGCCA CTGGCGGATC GGAGGGCAGG CGACGGGGGG CCATAGAGAC GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034058.1
CDS	244..3240
Translation	MFRSRSWFGGPWGGRPKNRLSLEHLKYLYSILEKNTTVSESNRGLLVESLRCIAEILIWGDQHDSLVFDFFLEKNMLSYFLHIMRQKSGGSSFVCVQLLQTLNILFENIRNETSLYYLLSNNHVNSIMVHKFDFSDEDVMGYYILFLKTLSLKLNTHTIHFFYNEHTNDFPLYTEAIKFFNHPESMVRIAVRTISLNVYKVQNPSMLRFIRDKTAAPYFSNLVWFIGKHILELDTCVRTDIDHQSNQKLSNLVAEHLDHLHYLSDILLLEIKDLNAVLTEHLLHKLFVPLYIFSLTPAPPPPSLAVVTQNLAAVLNRNVDIDIQEMHNPRVSSIVALFLLSLVFLVVSHAPLVHALAWVILNGDHSVFKEGAAEILNSYVEHREVVVPGFGEPDESLEQALDTVTGQSSSSSYALSEDSGVESSSPATTELDSQADAVEAEQIKLRNITDEEKQLLQKSSSSTKADFAEMAKPFLDTVLHALDCTENDYLALLSLCLIYAMSHNRGIKNEWFEQVLAKSTRGAFSYKTALIEHLLNIITQSSQPSSRIRLITVEIALELLVTFTRPSSDDSRCITAAQQDLLFSARNQSMVVIRNFYKSEDIFLDLFEDEYNEMRKAQLNVEFLCMDSTILLPPTGTPLTGINFTRRLPCGEVEKARRAIRVYFLLRRTCQKFLNEKESLLPLTNVVNLVQVENVLDLNNSDLIACTVVAKDSSKQRRFLVIDALQLILVEPDAKLLGWGVAKFVGFLQDVEVQGDKDDSRCLHITVHRGGVTHNRTPLLSAKFLFDDHIRCMAAKQRLTKGRSKARQKKMYQIAQLIEIPGQMDSPVYAVGGTMVASSSGGSGNSSGSSSRSSHHRPMFSTANRVPGFAAVLRGSNSAGVSRTQMAPNRSIEGSEEIPLEDFQHSRNNSPHSRGNPSPASRSHTPIRVLHYDQLSGHSGSPREASLGGTNALLSQLNGLNREVLPTQSSEETSFIGSDGNEATGGSEGRRRGAIETV

Target ORF information:

RefSeq Version	NM_001038969.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster endosomal maturation defective (ema), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038969.2

ORF Insert Sequence:

ATGTTCCGGA GTCGCAGCTG GTTTGGCGGA CCCTGGGGTG GGCGGCCCAA AAACCGCTTG 
TCGCTGGAGC ACCTTAAGTA CCTGTACAGC ATTCTGGAGA AGAACACCAC CGTGTCGGAA 
AGTAATCGTG GCCTGCTGGT GGAGTCGCTG CGCTGCATCG CAGAGATCCT GATTTGGGGC 
GACCAGCATG ACTCGCTGGT GTTTGACTTC TTCTTGGAGA AGAATATGCT ATCGTATTTC 
CTGCACATAA TGCGACAAAA GAGTGGAGGC TCGAGTTTCG TCTGCGTGCA GCTCCTTCAG 
ACTCTCAACA TCCTCTTTGA GAACATACGC AACGAGACGT CCCTGTATTA TCTGTTGAGC 
AACAACCATG TAAATTCTAT CATGGTGCAC AAGTTTGACT TCTCCGACGA GGATGTGATG 
GGCTATTATA TACTTTTTTT AAAGACGCTA AGTCTAAAGT TAAACACTCA CACAATACAC 
TTCTTTTACA ATGAGCACAC AAATGACTTT CCCTTGTATA CAGAGGCTAT TAAGTTCTTT 
AATCATCCCG AGTCAATGGT GAGAATTGCA GTGCGCACCA TTTCCTTAAA TGTCTACAAG 
GTTCAAAATC CAAGCATGTT GCGTTTTATT CGAGACAAAA CGGCTGCTCC ATATTTCAGT 
AATCTGGTCT GGTTTATCGG CAAACACATT CTTGAACTAG ACACTTGTGT TCGCACAGAT 
ATAGACCATC AATCGAATCA AAAGCTGTCC AATTTGGTTG CCGAGCATCT GGATCATTTG 
CATTATTTGA GTGACATTCT TCTACTGGAA ATCAAGGATC TGAACGCGGT GTTAACCGAG 
CACTTGCTGC ATAAGCTATT CGTGCCTCTG TACATATTCT CACTTACACC AGCTCCGCCC 
CCGCCCTCAC TGGCAGTGGT TACCCAGAAT CTAGCTGCTG TGCTTAATCG CAATGTGGAC 
ATCGACATAC AGGAGATGCA CAATCCGCGA GTGAGCTCCA TCGTGGCCCT TTTCCTGCTC 
TCATTGGTCT TCCTGGTGGT TTCGCATGCC CCTCTGGTGC ACGCTCTGGC CTGGGTTATC 
CTCAATGGTG ACCACAGCGT TTTTAAGGAG GGTGCAGCCG AGATTCTTAA TTCATACGTT 
GAGCACCGCG AGGTGGTTGT GCCTGGCTTC GGCGAGCCCG ACGAGAGTCT CGAACAGGCC 
TTGGATACAG TCACCGGTCA GTCCAGCTCG TCCAGCTATG CCCTTAGCGA GGACAGTGGC 
GTGGAGTCCT CATCCCCAGC CACCACAGAA CTGGACTCTC AAGCAGATGC GGTTGAAGCG 
GAGCAGATTA AACTGCGCAA CATAACTGAC GAGGAAAAGC AGCTGCTGCA GAAGTCATCT 
TCCTCAACGA AGGCTGACTT TGCTGAGATG GCCAAACCGT TTTTAGACAC GGTCCTGCAT 
GCTTTGGACT GCACCGAAAA CGATTACCTT GCCCTGCTGT CGCTGTGCCT CATATATGCT 
ATGTCGCACA ATAGAGGCAT CAAGAATGAG TGGTTCGAGC AGGTTCTTGC AAAATCAACC 
CGTGGCGCCT TCAGTTATAA GACAGCCTTG ATTGAGCATC TGTTAAACAT CATCACCCAG 
TCCAGTCAGC CATCGTCCCG CATTCGGTTG ATTACCGTGG AGATTGCCTT GGAGTTACTG 
GTCACTTTCA CCCGTCCCAG CTCGGATGAT TCGCGCTGCA TAACCGCCGC ACAACAGGAT 
CTGCTCTTCA GCGCCCGCAA TCAGTCGATG GTGGTGATCC GTAACTTCTA CAAGTCGGAG 
GACATCTTCC TAGACCTGTT CGAAGACGAA TACAACGAGA TGCGAAAGGC GCAACTGAAC 
GTGGAATTCC TGTGCATGGA CTCCACCATT CTACTGCCTC CCACGGGAAC GCCACTTACT 
GGAATCAACT TTACGCGACG ACTTCCCTGC GGGGAGGTGG AAAAGGCACG CCGAGCCATT 
CGCGTTTACT TCCTGCTGCG TCGCACGTGC CAAAAGTTTT TGAATGAGAA GGAATCGCTA 
TTGCCGCTTA CAAATGTAGT CAATCTGGTT CAGGTGGAGA ACGTACTCGA TCTGAACAAT 
AGTGATCTAA TAGCCTGCAC CGTTGTGGCC AAGGACAGCA GCAAGCAAAG ACGCTTTCTG 
GTCATTGACG CATTGCAGCT AATTCTCGTC GAGCCGGATG CAAAGCTGCT TGGCTGGGGC 
GTGGCAAAGT TCGTTGGCTT TCTGCAGGAC GTAGAGGTGC AGGGTGATAA GGATGACTCG 
CGCTGCTTGC ACATCACCGT GCACCGGGGC GGAGTCACTC ACAACCGCAC TCCGTTGCTC 
TCGGCTAAGT TCCTGTTCGA CGATCACATC CGTTGCATGG CTGCCAAGCA GAGGTTGACA 
AAGGGTCGTA GCAAGGCGCG ACAAAAGAAG ATGTACCAGA TTGCGCAACT CATCGAGATT 
CCTGGACAGA TGGACTCTCC CGTCTACGCT GTTGGTGGCA CAATGGTCGC ATCCAGTAGC 
GGCGGAAGTG GTAACAGCAG TGGCAGCAGT TCGCGCAGTA GTCACCACCG TCCCATGTTC 
TCCACGGCCA ATCGAGTGCC GGGATTTGCA GCGGTGCTGC GTGGAAGCAA TAGCGCTGGG 
GTTAGTCGAA CCCAGATGGC CCCGAATCGA TCCATCGAAG GGTCGGAGGA GATTCCTCTC 
GAGGACTTCC AGCACTCACG GAACAACAGT CCGCACTCTC GGGGAAACCC CTCGCCTGCC 
TCCCGCTCAC ACACACCCAT CCGCGTGCTC CACTACGATC AGCTGTCCGG TCATAGCGGC 
TCACCACGTG AAGCGTCCCT GGGGGGAACT AACGCCCTGC TAAGCCAGTT AAATGGGCTG 
AACCGTGAAG TGCTCCCAAC GCAGAGCTCC GAGGAGACTT CTTTTATTGG GAGCGACGGA 
AACGAGGCCA CTGGCGGATC GGAGGGCAGG CGACGGGGGG CCATAGAGAC GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ema ( NM_142285.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650542.1 Drosophila melanogaster endosomal maturation defective (ema), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05123
Clone ID Related Accession (Same CDS sequence)	NM_142285.2
Accession Version	NM_142285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	endosomal maturation defective, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142285.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCGGA GTCGCAGCTG GTTTGGCGGA CCCTGGGGTG GGCGGCCCAA AAACCGCTTG 
TCGCTGGAGC ACCTTAAGTA CCTGTACAGC ATTCTGGAGA AGAACACCAC CGTGTCGGAA 
AGTAATCGTG GCCTGCTGGT GGAGTCGCTG CGCTGCATCG CAGAGATCCT GATTTGGGGC 
GACCAGCATG ACTCGCTGGT GTTTGACTTC TTCTTGGAGA AGAATATGCT ATCGTATTTC 
CTGCACATAA TGCGACAAAA GAGTGGAGGC TCGAGTTTCG TCTGCGTGCA GCTCCTTCAG 
ACTCTCAACA TCCTCTTTGA GAACATACGC AACGAGACGT CCCTGTATTA TCTGTTGAGC 
AACAACCATG TAAATTCTAT CATGGTGCAC AAGTTTGACT TCTCCGACGA GGATGTGATG 
GGCTATTATA TACTTTTTTT AAAGACGCTA AGTCTAAAGT TAAACACTCA CACAATACAC 
TTCTTTTACA ATGAGCACAC AAATGACTTT CCCTTGTATA CAGAGGCTAT TAAGTTCTTT 
AATCATCCCG AGTCAATGGT GAGAATTGCA GTGCGCACCA TTTCCTTAAA TGTCTACAAG 
GTTCAAAATC CAAGCATGTT GCGTTTTATT CGAGACAAAA CGGCTGCTCC ATATTTCAGT 
AATCTGGTCT GGTTTATCGG CAAACACATT CTTGAACTAG ACACTTGTGT TCGCACAGAT 
ATAGACCATC AATCGAATCA AAAGCTGTCC AATTTGGTTG CCGAGCATCT GGATCATTTG 
CATTATTTGA GTGACATTCT TCTACTGGAA ATCAAGGATC TGAACGCGGT GTTAACCGAG 
CACTTGCTGC ATAAGCTATT CGTGCCTCTG TACATATTCT CACTTACACC AGCTCCGCCC 
CCGCCCTCAC TGGCAGTGGT TACCCAGAAT CTAGCTGCTG TGCTTAATCG CAATGTGGAC 
ATCGACATAC AGGAGATGCA CAATCCGCGA GTGAGCTCCA TCGTGGCCCT TTTCCTGCTC 
TCATTGGTCT TCCTGGTGGT TTCGCATGCC CCTCTGGTGC ACGCTCTGGC CTGGGTTATC 
CTCAATGGTG ACCACAGCGT TTTTAAGGAG GGTGCAGCCG AGATTCTTAA TTCATACGTT 
GAGCACCGCG AGGTGGTTGT GCCTGGCTTC GGCGAGCCCG ACGAGAGTCT CGAACAGGCC 
TTGGATACAG TCACCGGTCA GTCCAGCTCG TCCAGCTATG CCCTTAGCGA GGACAGTGGC 
GTGGAGTCCT CATCCCCAGC CACCACAGAA CTGGACTCTC AAGCAGATGC GGTTGAAGCG 
GAGCAGATTA AACTGCGCAA CATAACTGAC GAGGAAAAGC AGCTGCTGCA GAAGTCATCT 
TCCTCAACGA AGGCTGACTT TGCTGAGATG GCCAAACCGT TTTTAGACAC GGTCCTGCAT 
GCTTTGGACT GCACCGAAAA CGATTACCTT GCCCTGCTGT CGCTGTGCCT CATATATGCT 
ATGTCGCACA ATAGAGGCAT CAAGAATGAG TGGTTCGAGC AGGTTCTTGC AAAATCAACC 
CGTGGCGCCT TCAGTTATAA GACAGCCTTG ATTGAGCATC TGTTAAACAT CATCACCCAG 
TCCAGTCAGC CATCGTCCCG CATTCGGTTG ATTACCGTGG AGATTGCCTT GGAGTTACTG 
GTCACTTTCA CCCGTCCCAG CTCGGATGAT TCGCGCTGCA TAACCGCCGC ACAACAGGAT 
CTGCTCTTCA GCGCCCGCAA TCAGTCGATG GTGGTGATCC GTAACTTCTA CAAGTCGGAG 
GACATCTTCC TAGACCTGTT CGAAGACGAA TACAACGAGA TGCGAAAGGC GCAACTGAAC 
GTGGAATTCC TGTGCATGGA CTCCACCATT CTACTGCCTC CCACGGGAAC GCCACTTACT 
GGAATCAACT TTACGCGACG ACTTCCCTGC GGGGAGGTGG AAAAGGCACG CCGAGCCATT 
CGCGTTTACT TCCTGCTGCG TCGCACGTGC CAAAAGTTTT TGAATGAGAA GGAATCGCTA 
TTGCCGCTTA CAAATGTAGT CAATCTGGTT CAGGTGGAGA ACGTACTCGA TCTGAACAAT 
AGTGATCTAA TAGCCTGCAC CGTTGTGGCC AAGGACAGCA GCAAGCAAAG ACGCTTTCTG 
GTCATTGACG CATTGCAGCT AATTCTCGTC GAGCCGGATG CAAAGCTGCT TGGCTGGGGC 
GTGGCAAAGT TCGTTGGCTT TCTGCAGGAC GTAGAGGTGC AGGGTGATAA GGATGACTCG 
CGCTGCTTGC ACATCACCGT GCACCGGGGC GGAGTCACTC ACAACCGCAC TCCGTTGCTC 
TCGGCTAAGT TCCTGTTCGA CGATCACATC CGTTGCATGG CTGCCAAGCA GAGGTTGACA 
AAGGGTCGTA GCAAGGCGCG ACAAAAGAAG ATGTACCAGA TTGCGCAACT CATCGAGATT 
CCTGGACAGA TGGACTCTCC CGTCTACGCT GTTGGTGGCA CAATGGTCGC ATCCAGTAGC 
GGCGGAAGTG GTAACAGCAG TGGCAGCAGT TCGCGCAGTA GTCACCACCG TCCCATGTTC 
TCCACGGCCA ATCGAGTGCC GGGATTTGCA GCGGTGCTGC GTGGAAGCAA TAGCGCTGGG 
GTTAGTCGAA CCCAGATGGC CCCGAATCGA TCCATCGAAG GAATTAGAAA TGAAAGCGCT 
GGCCGCTCCC GACGCCGCAG TCCCAGCTCA ACTTCGGGCT CGAATCTTAG GGCCGATCAC 
TCGGATCGGG AGCGATCGCC CAGTGTTAGC ATGGGCAGCC ACAGCTCCTC GCAGTCTCGG 
GAAAACTCCC AGCCCAGAAG TACTGGAAAT CGGTCGCGGG AGAGCAGCCC TAGAATGCCC 
AGGCCGCGGT CGGAGGAGAT TCCTCTCGAG GACTTCCAGC ACTCACGGAA CAACAGTCCG 
CACTCTCGGG GAAACCCCTC GCCTGCCTCC CGCTCACACA CACCCATCCG CGTGCTCCAC 
TACGATCAGC TGTCCGGTCA TAGCGGCTCA CCACGTGAAG CGTCCCTGGG GGGAACTAAC 
GCCCTGCTAA GCCAGTTAAA TGGGCTGAAC CGTGAAGTGC TCCCAACGCA GAGCTCCGAG 
GAGACTTCTT TTATTGGGAG CGACGGAAAC GAGGCCACTG GCGGATCGGA GGGCAGGCGA 
CGGGGGGCCA TAGAGACGGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650542.1
CDS	244..3447
Translation	MFRSRSWFGGPWGGRPKNRLSLEHLKYLYSILEKNTTVSESNRGLLVESLRCIAEILIWGDQHDSLVFDFFLEKNMLSYFLHIMRQKSGGSSFVCVQLLQTLNILFENIRNETSLYYLLSNNHVNSIMVHKFDFSDEDVMGYYILFLKTLSLKLNTHTIHFFYNEHTNDFPLYTEAIKFFNHPESMVRIAVRTISLNVYKVQNPSMLRFIRDKTAAPYFSNLVWFIGKHILELDTCVRTDIDHQSNQKLSNLVAEHLDHLHYLSDILLLEIKDLNAVLTEHLLHKLFVPLYIFSLTPAPPPPSLAVVTQNLAAVLNRNVDIDIQEMHNPRVSSIVALFLLSLVFLVVSHAPLVHALAWVILNGDHSVFKEGAAEILNSYVEHREVVVPGFGEPDESLEQALDTVTGQSSSSSYALSEDSGVESSSPATTELDSQADAVEAEQIKLRNITDEEKQLLQKSSSSTKADFAEMAKPFLDTVLHALDCTENDYLALLSLCLIYAMSHNRGIKNEWFEQVLAKSTRGAFSYKTALIEHLLNIITQSSQPSSRIRLITVEIALELLVTFTRPSSDDSRCITAAQQDLLFSARNQSMVVIRNFYKSEDIFLDLFEDEYNEMRKAQLNVEFLCMDSTILLPPTGTPLTGINFTRRLPCGEVEKARRAIRVYFLLRRTCQKFLNEKESLLPLTNVVNLVQVENVLDLNNSDLIACTVVAKDSSKQRRFLVIDALQLILVEPDAKLLGWGVAKFVGFLQDVEVQGDKDDSRCLHITVHRGGVTHNRTPLLSAKFLFDDHIRCMAAKQRLTKGRSKARQKKMYQIAQLIEIPGQMDSPVYAVGGTMVASSSGGSGNSSGSSSRSSHHRPMFSTANRVPGFAAVLRGSNSAGVSRTQMAPNRSIEGIRNESAGRSRRRSPSSTSGSNLRADHSDRERSPSVSMGSHSSSQSRENSQPRSTGNRSRESSPRMPRPRSEEIPLEDFQHSRNNSPHSRGNPSPASRSHTPIRVLHYDQLSGHSGSPREASLGGTNALLSQLNGLNREVLPTQSSEETSFIGSDGNEATGGSEGRRRGAIETV

Target ORF information:

RefSeq Version	NM_142285.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster endosomal maturation defective (ema), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142285.2

ORF Insert Sequence:

ATGTTCCGGA GTCGCAGCTG GTTTGGCGGA CCCTGGGGTG GGCGGCCCAA AAACCGCTTG 
TCGCTGGAGC ACCTTAAGTA CCTGTACAGC ATTCTGGAGA AGAACACCAC CGTGTCGGAA 
AGTAATCGTG GCCTGCTGGT GGAGTCGCTG CGCTGCATCG CAGAGATCCT GATTTGGGGC 
GACCAGCATG ACTCGCTGGT GTTTGACTTC TTCTTGGAGA AGAATATGCT ATCGTATTTC 
CTGCACATAA TGCGACAAAA GAGTGGAGGC TCGAGTTTCG TCTGCGTGCA GCTCCTTCAG 
ACTCTCAACA TCCTCTTTGA GAACATACGC AACGAGACGT CCCTGTATTA TCTGTTGAGC 
AACAACCATG TAAATTCTAT CATGGTGCAC AAGTTTGACT TCTCCGACGA GGATGTGATG 
GGCTATTATA TACTTTTTTT AAAGACGCTA AGTCTAAAGT TAAACACTCA CACAATACAC 
TTCTTTTACA ATGAGCACAC AAATGACTTT CCCTTGTATA CAGAGGCTAT TAAGTTCTTT 
AATCATCCCG AGTCAATGGT GAGAATTGCA GTGCGCACCA TTTCCTTAAA TGTCTACAAG 
GTTCAAAATC CAAGCATGTT GCGTTTTATT CGAGACAAAA CGGCTGCTCC ATATTTCAGT 
AATCTGGTCT GGTTTATCGG CAAACACATT CTTGAACTAG ACACTTGTGT TCGCACAGAT 
ATAGACCATC AATCGAATCA AAAGCTGTCC AATTTGGTTG CCGAGCATCT GGATCATTTG 
CATTATTTGA GTGACATTCT TCTACTGGAA ATCAAGGATC TGAACGCGGT GTTAACCGAG 
CACTTGCTGC ATAAGCTATT CGTGCCTCTG TACATATTCT CACTTACACC AGCTCCGCCC 
CCGCCCTCAC TGGCAGTGGT TACCCAGAAT CTAGCTGCTG TGCTTAATCG CAATGTGGAC 
ATCGACATAC AGGAGATGCA CAATCCGCGA GTGAGCTCCA TCGTGGCCCT TTTCCTGCTC 
TCATTGGTCT TCCTGGTGGT TTCGCATGCC CCTCTGGTGC ACGCTCTGGC CTGGGTTATC 
CTCAATGGTG ACCACAGCGT TTTTAAGGAG GGTGCAGCCG AGATTCTTAA TTCATACGTT 
GAGCACCGCG AGGTGGTTGT GCCTGGCTTC GGCGAGCCCG ACGAGAGTCT CGAACAGGCC 
TTGGATACAG TCACCGGTCA GTCCAGCTCG TCCAGCTATG CCCTTAGCGA GGACAGTGGC 
GTGGAGTCCT CATCCCCAGC CACCACAGAA CTGGACTCTC AAGCAGATGC GGTTGAAGCG 
GAGCAGATTA AACTGCGCAA CATAACTGAC GAGGAAAAGC AGCTGCTGCA GAAGTCATCT 
TCCTCAACGA AGGCTGACTT TGCTGAGATG GCCAAACCGT TTTTAGACAC GGTCCTGCAT 
GCTTTGGACT GCACCGAAAA CGATTACCTT GCCCTGCTGT CGCTGTGCCT CATATATGCT 
ATGTCGCACA ATAGAGGCAT CAAGAATGAG TGGTTCGAGC AGGTTCTTGC AAAATCAACC 
CGTGGCGCCT TCAGTTATAA GACAGCCTTG ATTGAGCATC TGTTAAACAT CATCACCCAG 
TCCAGTCAGC CATCGTCCCG CATTCGGTTG ATTACCGTGG AGATTGCCTT GGAGTTACTG 
GTCACTTTCA CCCGTCCCAG CTCGGATGAT TCGCGCTGCA TAACCGCCGC ACAACAGGAT 
CTGCTCTTCA GCGCCCGCAA TCAGTCGATG GTGGTGATCC GTAACTTCTA CAAGTCGGAG 
GACATCTTCC TAGACCTGTT CGAAGACGAA TACAACGAGA TGCGAAAGGC GCAACTGAAC 
GTGGAATTCC TGTGCATGGA CTCCACCATT CTACTGCCTC CCACGGGAAC GCCACTTACT 
GGAATCAACT TTACGCGACG ACTTCCCTGC GGGGAGGTGG AAAAGGCACG CCGAGCCATT 
CGCGTTTACT TCCTGCTGCG TCGCACGTGC CAAAAGTTTT TGAATGAGAA GGAATCGCTA 
TTGCCGCTTA CAAATGTAGT CAATCTGGTT CAGGTGGAGA ACGTACTCGA TCTGAACAAT 
AGTGATCTAA TAGCCTGCAC CGTTGTGGCC AAGGACAGCA GCAAGCAAAG ACGCTTTCTG 
GTCATTGACG CATTGCAGCT AATTCTCGTC GAGCCGGATG CAAAGCTGCT TGGCTGGGGC 
GTGGCAAAGT TCGTTGGCTT TCTGCAGGAC GTAGAGGTGC AGGGTGATAA GGATGACTCG 
CGCTGCTTGC ACATCACCGT GCACCGGGGC GGAGTCACTC ACAACCGCAC TCCGTTGCTC 
TCGGCTAAGT TCCTGTTCGA CGATCACATC CGTTGCATGG CTGCCAAGCA GAGGTTGACA 
AAGGGTCGTA GCAAGGCGCG ACAAAAGAAG ATGTACCAGA TTGCGCAACT CATCGAGATT 
CCTGGACAGA TGGACTCTCC CGTCTACGCT GTTGGTGGCA CAATGGTCGC ATCCAGTAGC 
GGCGGAAGTG GTAACAGCAG TGGCAGCAGT TCGCGCAGTA GTCACCACCG TCCCATGTTC 
TCCACGGCCA ATCGAGTGCC GGGATTTGCA GCGGTGCTGC GTGGAAGCAA TAGCGCTGGG 
GTTAGTCGAA CCCAGATGGC CCCGAATCGA TCCATCGAAG GAATTAGAAA TGAAAGCGCT 
GGCCGCTCCC GACGCCGCAG TCCCAGCTCA ACTTCGGGCT CGAATCTTAG GGCCGATCAC 
TCGGATCGGG AGCGATCGCC CAGTGTTAGC ATGGGCAGCC ACAGCTCCTC GCAGTCTCGG 
GAAAACTCCC AGCCCAGAAG TACTGGAAAT CGGTCGCGGG AGAGCAGCCC TAGAATGCCC 
AGGCCGCGGT CGGAGGAGAT TCCTCTCGAG GACTTCCAGC ACTCACGGAA CAACAGTCCG 
CACTCTCGGG GAAACCCCTC GCCTGCCTCC CGCTCACACA CACCCATCCG CGTGCTCCAC 
TACGATCAGC TGTCCGGTCA TAGCGGCTCA CCACGTGAAG CGTCCCTGGG GGGAACTAAC 
GCCCTGCTAA GCCAGTTAAA TGGGCTGAAC CGTGAAGTGC TCCCAACGCA GAGCTCCGAG 
GAGACTTCTT TTATTGGGAG CGACGGAAAC GAGGCCACTG GCGGATCGGA GGGCAGGCGA 
CGGGGGGCCA TAGAGACGGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ema ( NM_001275706.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262635.1 Drosophila melanogaster endosomal maturation defective (ema), transcript variant C, mRNA.

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CloneID	OFa07929
Clone ID Related Accession (Same CDS sequence)	NM_001275706.1
Accession Version	NM_001275706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	endosomal maturation defective, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCGGA GTCGCAGCTG GTTTGGCGGA CCCTGGGGTG GGCGGCCCAA AAACCGCTTG 
TCGCTGGAGC ACCTTAAGTA CCTGTACAGC ATTCTGGAGA AGAACACCAC CGTGTCGGAA 
AGTAATCGTG GCCTGCTGGT GGAGTCGCTG CGCTGCATCG CAGAGATCCT GATTTGGGGC 
GACCAGCATG ACTCGCTGGT GTTTGACTTC TTCTTGGAGA AGAATATGCT ATCGTATTTC 
CTGCACATAA TGCGACAAAA GAGTGGAGGC TCGAGTTTCG TCTGCGTGCA GCTCCTTCAG 
ACTCTCAACA TCCTCTTTGA GAACATACGC AACGAGACGT CCCTGTATTA TCTGTTGAGC 
AACAACCATG TAAATTCTAT CATGGTGCAC AAGTTTGACT TCTCCGACGA GGATGTGATG 
GGCTATTATA TACTTTTTTT AAAGACGCTA AGTCTAAAGT TAAACACTCA CACAATACAC 
TTCTTTTACA ATGAGCACAC AAATGACTTT CCCTTGTATA CAGAGGCTAT TAAGTTCTTT 
AATCATCCCG AGTCAATGGT GAGAATTGCA GTGCGCACCA TTTCCTTAAA TGTCTACAAG 
GTTCAAAATC CAAGCATGTT GCGTTTTATT CGAGACAAAA CGGCTGCTCC ATATTTCAGT 
AATCTGGTCT GGTTTATCGG CAAACACATT CTTGAACTAG ACACTTGTGT TCGCACAGAT 
ATAGACCATC AATCGAATCA AAAGCTGTCC AATTTGGTTG CCGAGCATCT GGATCATTTG 
CATTATTTGA GTGACATTCT TCTACTGGAA ATCAAGGATC TGAACGCGGT GTTAACCGAG 
CACTTGCTGC ATAAGCTATT CGTGCCTCTG TACATATTCT CACTTACACC AGCTCCGCCC 
CCGCCCTCAC TGGCAGTGGT TACCCAGAAT CTAGCTGCTG TGCTTAATCG CAATGTGGAC 
ATCGACATAC AGGAGATGCA CAATCCGCGA GTGAGCTCCA TCGTGGCCCT TTTCCTGCTC 
TCATTGGTCT TCCTGGTGGT TTCGCATGCC CCTCTGGTGC ACGCTCTGGC CTGGGTTATC 
CTCAATGGTG ACCACAGCGT TTTTAAGGAG GGTGCAGCCG AGATTCTTAA TTCATACGTT 
GAGCACCGCG AGGTGGTTGT GCCTGGCTTC GGCGAGCCCG ACGAGAGTCT CGAACAGGCC 
TTGGATACAG TCACCGGTCA GTCCAGCTCG TCCAGCTATG CCCTTAGCGA GGACAGTGGC 
GTGGAGTCCT CATCCCCAGC CACCACAGAA CTGGACTCTC AAGCAGATGC GGTTGAAGCG 
GAGCAGATTA AACTGCGCAA CATAACTGAC GAGGAAAAGC AGCTGCTGCA GAAGTCATCT 
TCCTCAACGA AGGCTGACTT TGCTGAGATG GCCAAACCGT TTTTAGACAC GGTCCTGCAT 
GCTTTGGACT GCACCGAAAA CGATTACCTT GCCCTGCTGT CGCTGTGCCT CATATATGCT 
ATGTCGCACA ATAGAGCGTC CCGCATTCGG TTGATTACCG TGGAGATTGC CTTGGAGTTA 
CTGGTCACTT TCACCCGTCC CAGCTCGGAT GATTCGCGCT GCATAACCGC CGCACAACAG 
GATCTGCTCT TCAGCGCCCG CAATCAGTCG ATGGTGGTGA TCCGTAACTT CTACAAGTCG 
GAGGACATCT TCCTAGACCT GTTCGAAGAC GAATACAACG AGATGCGAAA GGCGCAACTG 
AACGTGGAAT TCCTGTGCAT GGACTCCACC ATTCTACTGC CTCCCACGGG AACGCCACTT 
ACTGGAATCA ACTTTACGCG ACGACTTCCC TGCGGGGAGG TGGAAAAGGC ACGCCGAGCC 
ATTCGCGTTT ACTTCCTGCT GCGTCGCACG TGCCAAAAGT TTTTGAATGA GAAGGAATCG 
CTATTGCCGC TTACAAATGT AGTCAATCTG GTTCAGGTGG AGAACGTACT CGATCTGAAC 
AATAGTGATC TAATAGCCTG CACCGTTGTG GCCAAGGACA GCAGCAAGCA AAGACGCTTT 
CTGGTCATTG ACGCATTGCA GCTAATTCTC GTCGAGCCGG ATGCAAAGCT GCTTGGCTGG 
GGCGTGGCAA AGTTCGTTGG CTTTCTGCAG GACGTAGAGG TGCAGGGTGA TAAGGATGAC 
TCGCGCTGCT TGCACATCAC CGTGCACCGG GGCGGAGTCA CTCACAACCG CACTCCGTTG 
CTCTCGGCTA AGTTCCTGTT CGACGATCAC ATCCGTTGCA TGGCTGCCAA GCAGAGGTTG 
ACAAAGGGTC GTAGCAAGGC GCGACAAAAG AAGATGTACC AGATTGCGCA ACTCATCGAG 
ATTCCTGGAC AGATGGACTC TCCCGTCTAC GCTGTTGGTG GCACAATGGT CGCATCCAGT 
AGCGGCGGAA GTGGTAACAG CAGTGGCAGC AGTTCGCGCA GTAGTCACCA CCGTCCCATG 
TTCTCCACGG CCAATCGAGT GCCGGGATTT GCAGCGGTGC TGCGTGGAAG CAATAGCGCT 
GGGGTTAGTC GAACCCAGAT GGCCCCGAAT CGATCCATCG AAGGAATTAG AAATGAAAGC 
GCTGGCCGCT CCCGACGCCG CAGTCCCAGC TCAACTTCGG GCTCGAATCT TAGGGCCGAT 
CACTCGGATC GGGAGCGATC GCCCAGTGTT AGCATGGGCA GCCACAGCTC CTCGCAGTCT 
CGGGAAAACT CCCAGCCCAG AAGTACTGGA AATCGGTCGC GGGAGAGCAG CCCTAGAATG 
CCCAGGCCGC GGTCGGAGGA GATTCCTCTC GAGGACTTCC AGCACTCACG GAACAACAGT 
CCGCACTCTC GGGGAAACCC CTCGCCTGCC TCCCGCTCAC ACACACCCAT CCGCGTGCTC 
CACTACGATC AGCTGTCCGG TCATAGCGGC TCACCACGTG AAGCGTCCCT GGGGGGAACT 
AACGCCCTGC TAAGCCAGTT AAATGGGCTG AACCGTGAAG TGCTCCCAAC GCAGAGCTCC 
GAGGAGACTT CTTTTATTGG GAGCGACGGA AACGAGGCCA CTGGCGGATC GGAGGGCAGG 
CGACGGGGGG CCATAGAGAC GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262635.1
CDS	244..3330
Translation	MFRSRSWFGGPWGGRPKNRLSLEHLKYLYSILEKNTTVSESNRGLLVESLRCIAEILIWGDQHDSLVFDFFLEKNMLSYFLHIMRQKSGGSSFVCVQLLQTLNILFENIRNETSLYYLLSNNHVNSIMVHKFDFSDEDVMGYYILFLKTLSLKLNTHTIHFFYNEHTNDFPLYTEAIKFFNHPESMVRIAVRTISLNVYKVQNPSMLRFIRDKTAAPYFSNLVWFIGKHILELDTCVRTDIDHQSNQKLSNLVAEHLDHLHYLSDILLLEIKDLNAVLTEHLLHKLFVPLYIFSLTPAPPPPSLAVVTQNLAAVLNRNVDIDIQEMHNPRVSSIVALFLLSLVFLVVSHAPLVHALAWVILNGDHSVFKEGAAEILNSYVEHREVVVPGFGEPDESLEQALDTVTGQSSSSSYALSEDSGVESSSPATTELDSQADAVEAEQIKLRNITDEEKQLLQKSSSSTKADFAEMAKPFLDTVLHALDCTENDYLALLSLCLIYAMSHNRASRIRLITVEIALELLVTFTRPSSDDSRCITAAQQDLLFSARNQSMVVIRNFYKSEDIFLDLFEDEYNEMRKAQLNVEFLCMDSTILLPPTGTPLTGINFTRRLPCGEVEKARRAIRVYFLLRRTCQKFLNEKESLLPLTNVVNLVQVENVLDLNNSDLIACTVVAKDSSKQRRFLVIDALQLILVEPDAKLLGWGVAKFVGFLQDVEVQGDKDDSRCLHITVHRGGVTHNRTPLLSAKFLFDDHIRCMAAKQRLTKGRSKARQKKMYQIAQLIEIPGQMDSPVYAVGGTMVASSSGGSGNSSGSSSRSSHHRPMFSTANRVPGFAAVLRGSNSAGVSRTQMAPNRSIEGIRNESAGRSRRRSPSSTSGSNLRADHSDRERSPSVSMGSHSSSQSRENSQPRSTGNRSRESSPRMPRPRSEEIPLEDFQHSRNNSPHSRGNPSPASRSHTPIRVLHYDQLSGHSGSPREASLGGTNALLSQLNGLNREVLPTQSSEETSFIGSDGNEATGGSEGRRRGAIETV

Target ORF information:

RefSeq Version	NM_001275706.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster endosomal maturation defective (ema), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275706.1

ORF Insert Sequence:

ATGTTCCGGA GTCGCAGCTG GTTTGGCGGA CCCTGGGGTG GGCGGCCCAA AAACCGCTTG 
TCGCTGGAGC ACCTTAAGTA CCTGTACAGC ATTCTGGAGA AGAACACCAC CGTGTCGGAA 
AGTAATCGTG GCCTGCTGGT GGAGTCGCTG CGCTGCATCG CAGAGATCCT GATTTGGGGC 
GACCAGCATG ACTCGCTGGT GTTTGACTTC TTCTTGGAGA AGAATATGCT ATCGTATTTC 
CTGCACATAA TGCGACAAAA GAGTGGAGGC TCGAGTTTCG TCTGCGTGCA GCTCCTTCAG 
ACTCTCAACA TCCTCTTTGA GAACATACGC AACGAGACGT CCCTGTATTA TCTGTTGAGC 
AACAACCATG TAAATTCTAT CATGGTGCAC AAGTTTGACT TCTCCGACGA GGATGTGATG 
GGCTATTATA TACTTTTTTT AAAGACGCTA AGTCTAAAGT TAAACACTCA CACAATACAC 
TTCTTTTACA ATGAGCACAC AAATGACTTT CCCTTGTATA CAGAGGCTAT TAAGTTCTTT 
AATCATCCCG AGTCAATGGT GAGAATTGCA GTGCGCACCA TTTCCTTAAA TGTCTACAAG 
GTTCAAAATC CAAGCATGTT GCGTTTTATT CGAGACAAAA CGGCTGCTCC ATATTTCAGT 
AATCTGGTCT GGTTTATCGG CAAACACATT CTTGAACTAG ACACTTGTGT TCGCACAGAT 
ATAGACCATC AATCGAATCA AAAGCTGTCC AATTTGGTTG CCGAGCATCT GGATCATTTG 
CATTATTTGA GTGACATTCT TCTACTGGAA ATCAAGGATC TGAACGCGGT GTTAACCGAG 
CACTTGCTGC ATAAGCTATT CGTGCCTCTG TACATATTCT CACTTACACC AGCTCCGCCC 
CCGCCCTCAC TGGCAGTGGT TACCCAGAAT CTAGCTGCTG TGCTTAATCG CAATGTGGAC 
ATCGACATAC AGGAGATGCA CAATCCGCGA GTGAGCTCCA TCGTGGCCCT TTTCCTGCTC 
TCATTGGTCT TCCTGGTGGT TTCGCATGCC CCTCTGGTGC ACGCTCTGGC CTGGGTTATC 
CTCAATGGTG ACCACAGCGT TTTTAAGGAG GGTGCAGCCG AGATTCTTAA TTCATACGTT 
GAGCACCGCG AGGTGGTTGT GCCTGGCTTC GGCGAGCCCG ACGAGAGTCT CGAACAGGCC 
TTGGATACAG TCACCGGTCA GTCCAGCTCG TCCAGCTATG CCCTTAGCGA GGACAGTGGC 
GTGGAGTCCT CATCCCCAGC CACCACAGAA CTGGACTCTC AAGCAGATGC GGTTGAAGCG 
GAGCAGATTA AACTGCGCAA CATAACTGAC GAGGAAAAGC AGCTGCTGCA GAAGTCATCT 
TCCTCAACGA AGGCTGACTT TGCTGAGATG GCCAAACCGT TTTTAGACAC GGTCCTGCAT 
GCTTTGGACT GCACCGAAAA CGATTACCTT GCCCTGCTGT CGCTGTGCCT CATATATGCT 
ATGTCGCACA ATAGAGCGTC CCGCATTCGG TTGATTACCG TGGAGATTGC CTTGGAGTTA 
CTGGTCACTT TCACCCGTCC CAGCTCGGAT GATTCGCGCT GCATAACCGC CGCACAACAG 
GATCTGCTCT TCAGCGCCCG CAATCAGTCG ATGGTGGTGA TCCGTAACTT CTACAAGTCG 
GAGGACATCT TCCTAGACCT GTTCGAAGAC GAATACAACG AGATGCGAAA GGCGCAACTG 
AACGTGGAAT TCCTGTGCAT GGACTCCACC ATTCTACTGC CTCCCACGGG AACGCCACTT 
ACTGGAATCA ACTTTACGCG ACGACTTCCC TGCGGGGAGG TGGAAAAGGC ACGCCGAGCC 
ATTCGCGTTT ACTTCCTGCT GCGTCGCACG TGCCAAAAGT TTTTGAATGA GAAGGAATCG 
CTATTGCCGC TTACAAATGT AGTCAATCTG GTTCAGGTGG AGAACGTACT CGATCTGAAC 
AATAGTGATC TAATAGCCTG CACCGTTGTG GCCAAGGACA GCAGCAAGCA AAGACGCTTT 
CTGGTCATTG ACGCATTGCA GCTAATTCTC GTCGAGCCGG ATGCAAAGCT GCTTGGCTGG 
GGCGTGGCAA AGTTCGTTGG CTTTCTGCAG GACGTAGAGG TGCAGGGTGA TAAGGATGAC 
TCGCGCTGCT TGCACATCAC CGTGCACCGG GGCGGAGTCA CTCACAACCG CACTCCGTTG 
CTCTCGGCTA AGTTCCTGTT CGACGATCAC ATCCGTTGCA TGGCTGCCAA GCAGAGGTTG 
ACAAAGGGTC GTAGCAAGGC GCGACAAAAG AAGATGTACC AGATTGCGCA ACTCATCGAG 
ATTCCTGGAC AGATGGACTC TCCCGTCTAC GCTGTTGGTG GCACAATGGT CGCATCCAGT 
AGCGGCGGAA GTGGTAACAG CAGTGGCAGC AGTTCGCGCA GTAGTCACCA CCGTCCCATG 
TTCTCCACGG CCAATCGAGT GCCGGGATTT GCAGCGGTGC TGCGTGGAAG CAATAGCGCT 
GGGGTTAGTC GAACCCAGAT GGCCCCGAAT CGATCCATCG AAGGAATTAG AAATGAAAGC 
GCTGGCCGCT CCCGACGCCG CAGTCCCAGC TCAACTTCGG GCTCGAATCT TAGGGCCGAT 
CACTCGGATC GGGAGCGATC GCCCAGTGTT AGCATGGGCA GCCACAGCTC CTCGCAGTCT 
CGGGAAAACT CCCAGCCCAG AAGTACTGGA AATCGGTCGC GGGAGAGCAG CCCTAGAATG 
CCCAGGCCGC GGTCGGAGGA GATTCCTCTC GAGGACTTCC AGCACTCACG GAACAACAGT 
CCGCACTCTC GGGGAAACCC CTCGCCTGCC TCCCGCTCAC ACACACCCAT CCGCGTGCTC 
CACTACGATC AGCTGTCCGG TCATAGCGGC TCACCACGTG AAGCGTCCCT GGGGGGAACT 
AACGCCCTGC TAAGCCAGTT AAATGGGCTG AACCGTGAAG TGCTCCCAAC GCAGAGCTCC 
GAGGAGACTT CTTTTATTGG GAGCGACGGA AACGAGGCCA CTGGCGGATC GGAGGGCAGG 
CGACGGGGGG CCATAGAGAC GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ema cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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