Akt1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Akt1
Entrez Gene ID	41957
Full Name	CG4006 gene product from transcript CG4006-RE
Synonyms	AKT,AKT/PKB,AKT1,Akt,Akt/PKB,Akt1,PKB,CG4006,D-Akt,DAKT1,DAKT1/PKB,DAkt,DAkt1,DPKB,DRAC-PK,DRAC-PK66,DRAC-PK85,Dakt,Dakt1,Dmel\CG4006,Dpkb,PKB,PKB/AKT,PKB/Akt,PKB/dAKT,RAC,RacPK,akt,akt1,dAKT,dAKT/dPKB,dAKT1,dAkt,dAkt/PKB,dAkt1,dPKB,dakt,dakt1,l(3)04226,l(3)89Bq,p-Akt,pAkt
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89B3-89B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_169706.2	NP_732114.1	Akt1, isoform A
NM_169705.2	NP_732113.3	Akt1, isoform C
NM_001300425.1	NP_001287354.1	Akt1, isoform E
NM_001300424.1	NP_001287353.1	Akt1, isoform D
NM_169707.2	NP_732115.1	Akt1, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	AKT2	NP_001617.1
Mus musculus (house mouse)	Akt2	NP_001103678.1
Bos taurus (cattle)	AKT2	NP_001193075.1
Danio rerio (zebrafish)	akt2	NP_937789.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002161	XP_308030.5
Rattus norvegicus (Norway rat)	Akt2	NP_058789.1
Pan troglodytes (chimpanzee)	AKT2	XP_001143709.2
	AKT2	XP_001093461.1
Canis lupus familiaris (dog)	AKT2	NP_001012340.1
Xenopus tropicalis (tropical clawed frog)	akt2	NP_001116936.1
Drosophila melanogaster (fruit fly)	Akt1	NP_732114.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings demonstrated that lovastatin restored LRRK2-G2019S neurite degeneration by augmenting Akt/NRF2 pathway and inhibiting downstream GSK3b activity, which decreased phospho-tau levels. We suggested that lovastatin is a potential disease-modifying agent for LRRK2-G2019S parkinsonism.
Title: Lovastatin protects neurite degeneration in LRRK2-G2019S parkinsonism through activating the Akt/Nrf pathway and inhibiting GSK3? activity.

subtle manipulation of foxo through Akt1 can enhance survival during adverse nutrient conditions in Drosophila.
Title: Enhanced survival of Drosophila Akt1 hypomorphs during amino-acid starvation requires foxo.

The metabolic defects of cycG mutant animals are abrogated by a concomitant loss of Wdb, CycG presumably influences Akt1 activity at the PP2A nexus; Well rounded (Wrd), another B' subunit of PP2A in Drosophila, binds CycG similar to Wdb, and that its loss ameliorates some, but not all, of the metabolic defects of cycG mutants.
Title: A triangular connection between Cyclin G, PP2A and Akt1 in the regulation of growth and metabolism in Drosophila.

The developmental delay of these novel Akt1 hypomorphs results in a latent phenotype uncovered by generation of somatic clones
Title: Compensatory growth in novel Drosophila Akt1 mutants.

AKT1 and caspase-dependent regulation of Acn stability adjusts basal autophagy levels.
Title: Acinus integrates AKT1 and subapoptotic caspase activities to regulate basal autophagy.

The following Akt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05087	NM_169706.2 Latest version!	Drosophila melanogaster Akt1, transcript variant A (Akt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05087	NM_169707.2 Latest version!	Drosophila melanogaster Akt1, transcript variant B (Akt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05087	NM_001300425.1 Latest version!	Drosophila melanogaster Akt1, transcript variant E (Akt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05087	NM_001300424.1 Latest version!	Drosophila melanogaster Akt1, transcript variant D (Akt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05088	NM_169705.2 Latest version!	Drosophila melanogaster Akt1, transcript variant C (Akt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akt1 ( NM_169706.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732114.1 Drosophila melanogaster Akt1, transcript variant A (Akt1), mRNA.

Price & Availability↑

CloneID	OFa05087
Clone ID Related Accession (Same CDS sequence)	NM_169706.2 , NM_001300425.1 , NM_001300424.1 , NM_169707.2
Accession Version	NM_169706.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Akt1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169706.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAATAA ACACAACTTT CGACCTCAGC TCGCCCAGCG TTACATCGGG TCATGCGCTT 
ACGGAACAGA CTCAGGTCGT AAAGGAGGGG TGGCTGATGA AGAGGGGCGA GCACATAAAG 
AACTGGCGCC AGCGCTACTT CGTGCTCCAC TCCGATGGAA GACTGATGGG TTACCGCAGC 
AAGCCGGCAG ATAGTGCCAG CACGCCATCG GACTTCCTGC TCAACAACTT TACGGTGCGC 
GGCTGCCAGA TCATGACCGT CGATCGGCCC AAGCCATTCA CCTTCATCAT CCGCGGCCTG 
CAATGGACCA CGGTAATCGA AAGGACATTT GCCGTCGAAT CAGAATTAGA GCGCCAGCAG 
TGGACGGAGG CCATTCGCAA CGTATCCAGC CGGCTCATAG ATGTGGGTGA GGTGGCTATG 
ACGCCATCTG AACAGACAGA CATGACAGAC GTGGACATGG CCACCATTGC CGAGGACGAG 
CTTTCCGAGC AGTTCTCCGT ACAGGGAACG ACTTGTAATA GCAGCGGCGT TAAGAAAGTG 
ACTTTGGAGA ATTTTGAGTT CCTCAAGGTG CTCGGCAAGG GCACCTTTGG TAAGGTTATT 
TTGTGTCGCG AGAAGGCTAC CGCCAAGCTG TACGCGATCA AAATTCTTAA AAAGGAAGTT 
ATTATCCAAA AGGACGAGGT GGCCCATACC CTGACCGAGA GTCGTGTGCT CAAGTCCACA 
AATCATCCGT TCCTCATTTC ACTCAAATAT TCGTTTCAAA CCAACGACCG CCTGTGTTTT 
GTGATGCAGT ACGTGAACGG TGGCGAGCTC TTTTGGCATT TAAGCCACGA ACGCATATTT 
ACTGAGGATC GAACACGTTT CTATGGGGCA GAGATCATTT CTGCTCTGGG CTATCTGCAT 
TCACAGGGCA TAATTTATCG CGACTTAAAG CTGGAAAATC TTTTGCTGGA CAAAGATGGT 
CACATAAAGG TCGCAGACTT CGGTCTGTGC AAGGAGGACA TCACCTACGG CCGCACAACG 
AAAACCTTCT GCGGTACACC GGAATACCTA GCTCCGGAAG TATTAGATGA CAATGATTAT 
GGCCAAGCGG TGGACTGGTG GGGCACTGGC GTGGTCATGT ACGAGATGAT CTGTGGTCGT 
CTTCCGTTTT ACAATCGTGA TCATGATGTG CTCTTTACAT TGATTTTGGT GGAGGAGGTA 
AAATTCCCGC GTAATATCAC AGATGAGGCG AAAAATCTGT TAGCAGGCCT TCTAGCTAAG 
GATCCCAAAA AGCGTTTGGG AGGTGGAAAG GATGATGTCA AGGAGATACA AGCACATCCA 
TTCTTTGCGA GTATTAACTG GACAGATCTA GTGTTGAAAA AAATACCGCC GCCTTTCAAG 
CCTCAGGTGA CATCCGATAC GGATACAAGG TACTTCGACA AGGAGTTTAC GGGAGAGAGT 
GTGGAGTTGA CGCCGCCGGA TCCCACTGGC CCGTTGGGCT CCATAGCCGA AGAGCCGCTT 
TTCCCGCAGT TCAGCTACCA AGGAGACATG GCCTCCACGT TGGGCACCTC GTCGCACATT 
AGTACTTCCA CAAGTCTCGC ATCGATGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732114.1
CDS	721..2313
Translation	MSINTTFDLSSPSVTSGHALTEQTQVVKEGWLMKRGEHIKNWRQRYFVLHSDGRLMGYRSKPADSASTPSDFLLNNFTVRGCQIMTVDRPKPFTFIIRGLQWTTVIERTFAVESELERQQWTEAIRNVSSRLIDVGEVAMTPSEQTDMTDVDMATIAEDELSEQFSVQGTTCNSSGVKKVTLENFEFLKVLGKGTFGKVILCREKATAKLYAIKILKKEVIIQKDEVAHTLTESRVLKSTNHPFLISLKYSFQTNDRLCFVMQYVNGGELFWHLSHERIFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFGLCKEDITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKRLGGGKDDVKEIQAHPFFASINWTDLVLKKIPPPFKPQVTSDTDTRYFDKEFTGESVELTPPDPTGPLGSIAEEPLFPQFSYQGDMASTLGTSSHISTSTSLASMQ

Target ORF information:

RefSeq Version	NM_169706.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Akt1, transcript variant A (Akt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169706.2

ORF Insert Sequence:

ATGTCAATAA ACACAACTTT CGACCTCAGC TCGCCCAGCG TTACATCGGG TCATGCGCTT 
ACGGAACAGA CTCAGGTCGT AAAGGAGGGG TGGCTGATGA AGAGGGGCGA GCACATAAAG 
AACTGGCGCC AGCGCTACTT CGTGCTCCAC TCCGATGGAA GACTGATGGG TTACCGCAGC 
AAGCCGGCAG ATAGTGCCAG CACGCCATCG GACTTCCTGC TCAACAACTT TACGGTGCGC 
GGCTGCCAGA TCATGACCGT CGATCGGCCC AAGCCATTCA CCTTCATCAT CCGCGGCCTG 
CAATGGACCA CGGTAATCGA AAGGACATTT GCCGTCGAAT CAGAATTAGA GCGCCAGCAG 
TGGACGGAGG CCATTCGCAA CGTATCCAGC CGGCTCATAG ATGTGGGTGA GGTGGCTATG 
ACGCCATCTG AACAGACAGA CATGACAGAC GTGGACATGG CCACCATTGC CGAGGACGAG 
CTTTCCGAGC AGTTCTCCGT ACAGGGAACG ACTTGTAATA GCAGCGGCGT TAAGAAAGTG 
ACTTTGGAGA ATTTTGAGTT CCTCAAGGTG CTCGGCAAGG GCACCTTTGG TAAGGTTATT 
TTGTGTCGCG AGAAGGCTAC CGCCAAGCTG TACGCGATCA AAATTCTTAA AAAGGAAGTT 
ATTATCCAAA AGGACGAGGT GGCCCATACC CTGACCGAGA GTCGTGTGCT CAAGTCCACA 
AATCATCCGT TCCTCATTTC ACTCAAATAT TCGTTTCAAA CCAACGACCG CCTGTGTTTT 
GTGATGCAGT ACGTGAACGG TGGCGAGCTC TTTTGGCATT TAAGCCACGA ACGCATATTT 
ACTGAGGATC GAACACGTTT CTATGGGGCA GAGATCATTT CTGCTCTGGG CTATCTGCAT 
TCACAGGGCA TAATTTATCG CGACTTAAAG CTGGAAAATC TTTTGCTGGA CAAAGATGGT 
CACATAAAGG TCGCAGACTT CGGTCTGTGC AAGGAGGACA TCACCTACGG CCGCACAACG 
AAAACCTTCT GCGGTACACC GGAATACCTA GCTCCGGAAG TATTAGATGA CAATGATTAT 
GGCCAAGCGG TGGACTGGTG GGGCACTGGC GTGGTCATGT ACGAGATGAT CTGTGGTCGT 
CTTCCGTTTT ACAATCGTGA TCATGATGTG CTCTTTACAT TGATTTTGGT GGAGGAGGTA 
AAATTCCCGC GTAATATCAC AGATGAGGCG AAAAATCTGT TAGCAGGCCT TCTAGCTAAG 
GATCCCAAAA AGCGTTTGGG AGGTGGAAAG GATGATGTCA AGGAGATACA AGCACATCCA 
TTCTTTGCGA GTATTAACTG GACAGATCTA GTGTTGAAAA AAATACCGCC GCCTTTCAAG 
CCTCAGGTGA CATCCGATAC GGATACAAGG TACTTCGACA AGGAGTTTAC GGGAGAGAGT 
GTGGAGTTGA CGCCGCCGGA TCCCACTGGC CCGTTGGGCT CCATAGCCGA AGAGCCGCTT 
TTCCCGCAGT TCAGCTACCA AGGAGACATG GCCTCCACGT TGGGCACCTC GTCGCACATT 
AGTACTTCCA CAAGTCTCGC ATCGATGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akt1 ( NM_001300425.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287354.1 Drosophila melanogaster Akt1, transcript variant E (Akt1), mRNA.

Price & Availability↑

CloneID	OFa05087
Clone ID Related Accession (Same CDS sequence)	NM_169706.2 , NM_001300425.1 , NM_001300424.1 , NM_169707.2
Accession Version	NM_001300425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Akt1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAATAA ACACAACTTT CGACCTCAGC TCGCCCAGCG TTACATCGGG TCATGCGCTT 
ACGGAACAGA CTCAGGTCGT AAAGGAGGGG TGGCTGATGA AGAGGGGCGA GCACATAAAG 
AACTGGCGCC AGCGCTACTT CGTGCTCCAC TCCGATGGAA GACTGATGGG TTACCGCAGC 
AAGCCGGCAG ATAGTGCCAG CACGCCATCG GACTTCCTGC TCAACAACTT TACGGTGCGC 
GGCTGCCAGA TCATGACCGT CGATCGGCCC AAGCCATTCA CCTTCATCAT CCGCGGCCTG 
CAATGGACCA CGGTAATCGA AAGGACATTT GCCGTCGAAT CAGAATTAGA GCGCCAGCAG 
TGGACGGAGG CCATTCGCAA CGTATCCAGC CGGCTCATAG ATGTGGGTGA GGTGGCTATG 
ACGCCATCTG AACAGACAGA CATGACAGAC GTGGACATGG CCACCATTGC CGAGGACGAG 
CTTTCCGAGC AGTTCTCCGT ACAGGGAACG ACTTGTAATA GCAGCGGCGT TAAGAAAGTG 
ACTTTGGAGA ATTTTGAGTT CCTCAAGGTG CTCGGCAAGG GCACCTTTGG TAAGGTTATT 
TTGTGTCGCG AGAAGGCTAC CGCCAAGCTG TACGCGATCA AAATTCTTAA AAAGGAAGTT 
ATTATCCAAA AGGACGAGGT GGCCCATACC CTGACCGAGA GTCGTGTGCT CAAGTCCACA 
AATCATCCGT TCCTCATTTC ACTCAAATAT TCGTTTCAAA CCAACGACCG CCTGTGTTTT 
GTGATGCAGT ACGTGAACGG TGGCGAGCTC TTTTGGCATT TAAGCCACGA ACGCATATTT 
ACTGAGGATC GAACACGTTT CTATGGGGCA GAGATCATTT CTGCTCTGGG CTATCTGCAT 
TCACAGGGCA TAATTTATCG CGACTTAAAG CTGGAAAATC TTTTGCTGGA CAAAGATGGT 
CACATAAAGG TCGCAGACTT CGGTCTGTGC AAGGAGGACA TCACCTACGG CCGCACAACG 
AAAACCTTCT GCGGTACACC GGAATACCTA GCTCCGGAAG TATTAGATGA CAATGATTAT 
GGCCAAGCGG TGGACTGGTG GGGCACTGGC GTGGTCATGT ACGAGATGAT CTGTGGTCGT 
CTTCCGTTTT ACAATCGTGA TCATGATGTG CTCTTTACAT TGATTTTGGT GGAGGAGGTA 
AAATTCCCGC GTAATATCAC AGATGAGGCG AAAAATCTGT TAGCAGGCCT TCTAGCTAAG 
GATCCCAAAA AGCGTTTGGG AGGTGGAAAG GATGATGTCA AGGAGATACA AGCACATCCA 
TTCTTTGCGA GTATTAACTG GACAGATCTA GTGTTGAAAA AAATACCGCC GCCTTTCAAG 
CCTCAGGTGA CATCCGATAC GGATACAAGG TACTTCGACA AGGAGTTTAC GGGAGAGAGT 
GTGGAGTTGA CGCCGCCGGA TCCCACTGGC CCGTTGGGCT CCATAGCCGA AGAGCCGCTT 
TTCCCGCAGT TCAGCTACCA AGGAGACATG GCCTCCACGT TGGGCACCTC GTCGCACATT 
AGTACTTCCA CAAGTCTCGC ATCGATGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287354.1
CDS	721..2313
Translation	MSINTTFDLSSPSVTSGHALTEQTQVVKEGWLMKRGEHIKNWRQRYFVLHSDGRLMGYRSKPADSASTPSDFLLNNFTVRGCQIMTVDRPKPFTFIIRGLQWTTVIERTFAVESELERQQWTEAIRNVSSRLIDVGEVAMTPSEQTDMTDVDMATIAEDELSEQFSVQGTTCNSSGVKKVTLENFEFLKVLGKGTFGKVILCREKATAKLYAIKILKKEVIIQKDEVAHTLTESRVLKSTNHPFLISLKYSFQTNDRLCFVMQYVNGGELFWHLSHERIFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFGLCKEDITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKRLGGGKDDVKEIQAHPFFASINWTDLVLKKIPPPFKPQVTSDTDTRYFDKEFTGESVELTPPDPTGPLGSIAEEPLFPQFSYQGDMASTLGTSSHISTSTSLASMQ

Target ORF information:

RefSeq Version	NM_001300425.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Akt1, transcript variant E (Akt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300425.1

ORF Insert Sequence:

ATGTCAATAA ACACAACTTT CGACCTCAGC TCGCCCAGCG TTACATCGGG TCATGCGCTT 
ACGGAACAGA CTCAGGTCGT AAAGGAGGGG TGGCTGATGA AGAGGGGCGA GCACATAAAG 
AACTGGCGCC AGCGCTACTT CGTGCTCCAC TCCGATGGAA GACTGATGGG TTACCGCAGC 
AAGCCGGCAG ATAGTGCCAG CACGCCATCG GACTTCCTGC TCAACAACTT TACGGTGCGC 
GGCTGCCAGA TCATGACCGT CGATCGGCCC AAGCCATTCA CCTTCATCAT CCGCGGCCTG 
CAATGGACCA CGGTAATCGA AAGGACATTT GCCGTCGAAT CAGAATTAGA GCGCCAGCAG 
TGGACGGAGG CCATTCGCAA CGTATCCAGC CGGCTCATAG ATGTGGGTGA GGTGGCTATG 
ACGCCATCTG AACAGACAGA CATGACAGAC GTGGACATGG CCACCATTGC CGAGGACGAG 
CTTTCCGAGC AGTTCTCCGT ACAGGGAACG ACTTGTAATA GCAGCGGCGT TAAGAAAGTG 
ACTTTGGAGA ATTTTGAGTT CCTCAAGGTG CTCGGCAAGG GCACCTTTGG TAAGGTTATT 
TTGTGTCGCG AGAAGGCTAC CGCCAAGCTG TACGCGATCA AAATTCTTAA AAAGGAAGTT 
ATTATCCAAA AGGACGAGGT GGCCCATACC CTGACCGAGA GTCGTGTGCT CAAGTCCACA 
AATCATCCGT TCCTCATTTC ACTCAAATAT TCGTTTCAAA CCAACGACCG CCTGTGTTTT 
GTGATGCAGT ACGTGAACGG TGGCGAGCTC TTTTGGCATT TAAGCCACGA ACGCATATTT 
ACTGAGGATC GAACACGTTT CTATGGGGCA GAGATCATTT CTGCTCTGGG CTATCTGCAT 
TCACAGGGCA TAATTTATCG CGACTTAAAG CTGGAAAATC TTTTGCTGGA CAAAGATGGT 
CACATAAAGG TCGCAGACTT CGGTCTGTGC AAGGAGGACA TCACCTACGG CCGCACAACG 
AAAACCTTCT GCGGTACACC GGAATACCTA GCTCCGGAAG TATTAGATGA CAATGATTAT 
GGCCAAGCGG TGGACTGGTG GGGCACTGGC GTGGTCATGT ACGAGATGAT CTGTGGTCGT 
CTTCCGTTTT ACAATCGTGA TCATGATGTG CTCTTTACAT TGATTTTGGT GGAGGAGGTA 
AAATTCCCGC GTAATATCAC AGATGAGGCG AAAAATCTGT TAGCAGGCCT TCTAGCTAAG 
GATCCCAAAA AGCGTTTGGG AGGTGGAAAG GATGATGTCA AGGAGATACA AGCACATCCA 
TTCTTTGCGA GTATTAACTG GACAGATCTA GTGTTGAAAA AAATACCGCC GCCTTTCAAG 
CCTCAGGTGA CATCCGATAC GGATACAAGG TACTTCGACA AGGAGTTTAC GGGAGAGAGT 
GTGGAGTTGA CGCCGCCGGA TCCCACTGGC CCGTTGGGCT CCATAGCCGA AGAGCCGCTT 
TTCCCGCAGT TCAGCTACCA AGGAGACATG GCCTCCACGT TGGGCACCTC GTCGCACATT 
AGTACTTCCA CAAGTCTCGC ATCGATGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akt1 ( NM_001300424.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287353.1 Drosophila melanogaster Akt1, transcript variant D (Akt1), mRNA.

Price & Availability↑

CloneID	OFa05087
Clone ID Related Accession (Same CDS sequence)	NM_169706.2 , NM_001300425.1 , NM_001300424.1 , NM_169707.2
Accession Version	NM_001300424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Akt1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAATAA ACACAACTTT CGACCTCAGC TCGCCCAGCG TTACATCGGG TCATGCGCTT 
ACGGAACAGA CTCAGGTCGT AAAGGAGGGG TGGCTGATGA AGAGGGGCGA GCACATAAAG 
AACTGGCGCC AGCGCTACTT CGTGCTCCAC TCCGATGGAA GACTGATGGG TTACCGCAGC 
AAGCCGGCAG ATAGTGCCAG CACGCCATCG GACTTCCTGC TCAACAACTT TACGGTGCGC 
GGCTGCCAGA TCATGACCGT CGATCGGCCC AAGCCATTCA CCTTCATCAT CCGCGGCCTG 
CAATGGACCA CGGTAATCGA AAGGACATTT GCCGTCGAAT CAGAATTAGA GCGCCAGCAG 
TGGACGGAGG CCATTCGCAA CGTATCCAGC CGGCTCATAG ATGTGGGTGA GGTGGCTATG 
ACGCCATCTG AACAGACAGA CATGACAGAC GTGGACATGG CCACCATTGC CGAGGACGAG 
CTTTCCGAGC AGTTCTCCGT ACAGGGAACG ACTTGTAATA GCAGCGGCGT TAAGAAAGTG 
ACTTTGGAGA ATTTTGAGTT CCTCAAGGTG CTCGGCAAGG GCACCTTTGG TAAGGTTATT 
TTGTGTCGCG AGAAGGCTAC CGCCAAGCTG TACGCGATCA AAATTCTTAA AAAGGAAGTT 
ATTATCCAAA AGGACGAGGT GGCCCATACC CTGACCGAGA GTCGTGTGCT CAAGTCCACA 
AATCATCCGT TCCTCATTTC ACTCAAATAT TCGTTTCAAA CCAACGACCG CCTGTGTTTT 
GTGATGCAGT ACGTGAACGG TGGCGAGCTC TTTTGGCATT TAAGCCACGA ACGCATATTT 
ACTGAGGATC GAACACGTTT CTATGGGGCA GAGATCATTT CTGCTCTGGG CTATCTGCAT 
TCACAGGGCA TAATTTATCG CGACTTAAAG CTGGAAAATC TTTTGCTGGA CAAAGATGGT 
CACATAAAGG TCGCAGACTT CGGTCTGTGC AAGGAGGACA TCACCTACGG CCGCACAACG 
AAAACCTTCT GCGGTACACC GGAATACCTA GCTCCGGAAG TATTAGATGA CAATGATTAT 
GGCCAAGCGG TGGACTGGTG GGGCACTGGC GTGGTCATGT ACGAGATGAT CTGTGGTCGT 
CTTCCGTTTT ACAATCGTGA TCATGATGTG CTCTTTACAT TGATTTTGGT GGAGGAGGTA 
AAATTCCCGC GTAATATCAC AGATGAGGCG AAAAATCTGT TAGCAGGCCT TCTAGCTAAG 
GATCCCAAAA AGCGTTTGGG AGGTGGAAAG GATGATGTCA AGGAGATACA AGCACATCCA 
TTCTTTGCGA GTATTAACTG GACAGATCTA GTGTTGAAAA AAATACCGCC GCCTTTCAAG 
CCTCAGGTGA CATCCGATAC GGATACAAGG TACTTCGACA AGGAGTTTAC GGGAGAGAGT 
GTGGAGTTGA CGCCGCCGGA TCCCACTGGC CCGTTGGGCT CCATAGCCGA AGAGCCGCTT 
TTCCCGCAGT TCAGCTACCA AGGAGACATG GCCTCCACGT TGGGCACCTC GTCGCACATT 
AGTACTTCCA CAAGTCTCGC ATCGATGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287353.1
CDS	308..1900
Translation	MSINTTFDLSSPSVTSGHALTEQTQVVKEGWLMKRGEHIKNWRQRYFVLHSDGRLMGYRSKPADSASTPSDFLLNNFTVRGCQIMTVDRPKPFTFIIRGLQWTTVIERTFAVESELERQQWTEAIRNVSSRLIDVGEVAMTPSEQTDMTDVDMATIAEDELSEQFSVQGTTCNSSGVKKVTLENFEFLKVLGKGTFGKVILCREKATAKLYAIKILKKEVIIQKDEVAHTLTESRVLKSTNHPFLISLKYSFQTNDRLCFVMQYVNGGELFWHLSHERIFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFGLCKEDITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKRLGGGKDDVKEIQAHPFFASINWTDLVLKKIPPPFKPQVTSDTDTRYFDKEFTGESVELTPPDPTGPLGSIAEEPLFPQFSYQGDMASTLGTSSHISTSTSLASMQ

Target ORF information:

RefSeq Version	NM_001300424.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Akt1, transcript variant D (Akt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300424.1

ORF Insert Sequence:

ATGTCAATAA ACACAACTTT CGACCTCAGC TCGCCCAGCG TTACATCGGG TCATGCGCTT 
ACGGAACAGA CTCAGGTCGT AAAGGAGGGG TGGCTGATGA AGAGGGGCGA GCACATAAAG 
AACTGGCGCC AGCGCTACTT CGTGCTCCAC TCCGATGGAA GACTGATGGG TTACCGCAGC 
AAGCCGGCAG ATAGTGCCAG CACGCCATCG GACTTCCTGC TCAACAACTT TACGGTGCGC 
GGCTGCCAGA TCATGACCGT CGATCGGCCC AAGCCATTCA CCTTCATCAT CCGCGGCCTG 
CAATGGACCA CGGTAATCGA AAGGACATTT GCCGTCGAAT CAGAATTAGA GCGCCAGCAG 
TGGACGGAGG CCATTCGCAA CGTATCCAGC CGGCTCATAG ATGTGGGTGA GGTGGCTATG 
ACGCCATCTG AACAGACAGA CATGACAGAC GTGGACATGG CCACCATTGC CGAGGACGAG 
CTTTCCGAGC AGTTCTCCGT ACAGGGAACG ACTTGTAATA GCAGCGGCGT TAAGAAAGTG 
ACTTTGGAGA ATTTTGAGTT CCTCAAGGTG CTCGGCAAGG GCACCTTTGG TAAGGTTATT 
TTGTGTCGCG AGAAGGCTAC CGCCAAGCTG TACGCGATCA AAATTCTTAA AAAGGAAGTT 
ATTATCCAAA AGGACGAGGT GGCCCATACC CTGACCGAGA GTCGTGTGCT CAAGTCCACA 
AATCATCCGT TCCTCATTTC ACTCAAATAT TCGTTTCAAA CCAACGACCG CCTGTGTTTT 
GTGATGCAGT ACGTGAACGG TGGCGAGCTC TTTTGGCATT TAAGCCACGA ACGCATATTT 
ACTGAGGATC GAACACGTTT CTATGGGGCA GAGATCATTT CTGCTCTGGG CTATCTGCAT 
TCACAGGGCA TAATTTATCG CGACTTAAAG CTGGAAAATC TTTTGCTGGA CAAAGATGGT 
CACATAAAGG TCGCAGACTT CGGTCTGTGC AAGGAGGACA TCACCTACGG CCGCACAACG 
AAAACCTTCT GCGGTACACC GGAATACCTA GCTCCGGAAG TATTAGATGA CAATGATTAT 
GGCCAAGCGG TGGACTGGTG GGGCACTGGC GTGGTCATGT ACGAGATGAT CTGTGGTCGT 
CTTCCGTTTT ACAATCGTGA TCATGATGTG CTCTTTACAT TGATTTTGGT GGAGGAGGTA 
AAATTCCCGC GTAATATCAC AGATGAGGCG AAAAATCTGT TAGCAGGCCT TCTAGCTAAG 
GATCCCAAAA AGCGTTTGGG AGGTGGAAAG GATGATGTCA AGGAGATACA AGCACATCCA 
TTCTTTGCGA GTATTAACTG GACAGATCTA GTGTTGAAAA AAATACCGCC GCCTTTCAAG 
CCTCAGGTGA CATCCGATAC GGATACAAGG TACTTCGACA AGGAGTTTAC GGGAGAGAGT 
GTGGAGTTGA CGCCGCCGGA TCCCACTGGC CCGTTGGGCT CCATAGCCGA AGAGCCGCTT 
TTCCCGCAGT TCAGCTACCA AGGAGACATG GCCTCCACGT TGGGCACCTC GTCGCACATT 
AGTACTTCCA CAAGTCTCGC ATCGATGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akt1 ( NM_169707.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732115.1 Drosophila melanogaster Akt1, transcript variant B (Akt1), mRNA.

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CloneID	OFa05087
Clone ID Related Accession (Same CDS sequence)	NM_169706.2 , NM_001300425.1 , NM_001300424.1 , NM_169707.2
Accession Version	NM_169707.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Akt1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169707.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAATAA ACACAACTTT CGACCTCAGC TCGCCCAGCG TTACATCGGG TCATGCGCTT 
ACGGAACAGA CTCAGGTCGT AAAGGAGGGG TGGCTGATGA AGAGGGGCGA GCACATAAAG 
AACTGGCGCC AGCGCTACTT CGTGCTCCAC TCCGATGGAA GACTGATGGG TTACCGCAGC 
AAGCCGGCAG ATAGTGCCAG CACGCCATCG GACTTCCTGC TCAACAACTT TACGGTGCGC 
GGCTGCCAGA TCATGACCGT CGATCGGCCC AAGCCATTCA CCTTCATCAT CCGCGGCCTG 
CAATGGACCA CGGTAATCGA AAGGACATTT GCCGTCGAAT CAGAATTAGA GCGCCAGCAG 
TGGACGGAGG CCATTCGCAA CGTATCCAGC CGGCTCATAG ATGTGGGTGA GGTGGCTATG 
ACGCCATCTG AACAGACAGA CATGACAGAC GTGGACATGG CCACCATTGC CGAGGACGAG 
CTTTCCGAGC AGTTCTCCGT ACAGGGAACG ACTTGTAATA GCAGCGGCGT TAAGAAAGTG 
ACTTTGGAGA ATTTTGAGTT CCTCAAGGTG CTCGGCAAGG GCACCTTTGG TAAGGTTATT 
TTGTGTCGCG AGAAGGCTAC CGCCAAGCTG TACGCGATCA AAATTCTTAA AAAGGAAGTT 
ATTATCCAAA AGGACGAGGT GGCCCATACC CTGACCGAGA GTCGTGTGCT CAAGTCCACA 
AATCATCCGT TCCTCATTTC ACTCAAATAT TCGTTTCAAA CCAACGACCG CCTGTGTTTT 
GTGATGCAGT ACGTGAACGG TGGCGAGCTC TTTTGGCATT TAAGCCACGA ACGCATATTT 
ACTGAGGATC GAACACGTTT CTATGGGGCA GAGATCATTT CTGCTCTGGG CTATCTGCAT 
TCACAGGGCA TAATTTATCG CGACTTAAAG CTGGAAAATC TTTTGCTGGA CAAAGATGGT 
CACATAAAGG TCGCAGACTT CGGTCTGTGC AAGGAGGACA TCACCTACGG CCGCACAACG 
AAAACCTTCT GCGGTACACC GGAATACCTA GCTCCGGAAG TATTAGATGA CAATGATTAT 
GGCCAAGCGG TGGACTGGTG GGGCACTGGC GTGGTCATGT ACGAGATGAT CTGTGGTCGT 
CTTCCGTTTT ACAATCGTGA TCATGATGTG CTCTTTACAT TGATTTTGGT GGAGGAGGTA 
AAATTCCCGC GTAATATCAC AGATGAGGCG AAAAATCTGT TAGCAGGCCT TCTAGCTAAG 
GATCCCAAAA AGCGTTTGGG AGGTGGAAAG GATGATGTCA AGGAGATACA AGCACATCCA 
TTCTTTGCGA GTATTAACTG GACAGATCTA GTGTTGAAAA AAATACCGCC GCCTTTCAAG 
CCTCAGGTGA CATCCGATAC GGATACAAGG TACTTCGACA AGGAGTTTAC GGGAGAGAGT 
GTGGAGTTGA CGCCGCCGGA TCCCACTGGC CCGTTGGGCT CCATAGCCGA AGAGCCGCTT 
TTCCCGCAGT TCAGCTACCA AGGAGACATG GCCTCCACGT TGGGCACCTC GTCGCACATT 
AGTACTTCCA CAAGTCTCGC ATCGATGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732115.1
CDS	656..2248
Translation	MSINTTFDLSSPSVTSGHALTEQTQVVKEGWLMKRGEHIKNWRQRYFVLHSDGRLMGYRSKPADSASTPSDFLLNNFTVRGCQIMTVDRPKPFTFIIRGLQWTTVIERTFAVESELERQQWTEAIRNVSSRLIDVGEVAMTPSEQTDMTDVDMATIAEDELSEQFSVQGTTCNSSGVKKVTLENFEFLKVLGKGTFGKVILCREKATAKLYAIKILKKEVIIQKDEVAHTLTESRVLKSTNHPFLISLKYSFQTNDRLCFVMQYVNGGELFWHLSHERIFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFGLCKEDITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKRLGGGKDDVKEIQAHPFFASINWTDLVLKKIPPPFKPQVTSDTDTRYFDKEFTGESVELTPPDPTGPLGSIAEEPLFPQFSYQGDMASTLGTSSHISTSTSLASMQ

Target ORF information:

RefSeq Version	NM_169707.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Akt1, transcript variant B (Akt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169707.2

ORF Insert Sequence:

ATGTCAATAA ACACAACTTT CGACCTCAGC TCGCCCAGCG TTACATCGGG TCATGCGCTT 
ACGGAACAGA CTCAGGTCGT AAAGGAGGGG TGGCTGATGA AGAGGGGCGA GCACATAAAG 
AACTGGCGCC AGCGCTACTT CGTGCTCCAC TCCGATGGAA GACTGATGGG TTACCGCAGC 
AAGCCGGCAG ATAGTGCCAG CACGCCATCG GACTTCCTGC TCAACAACTT TACGGTGCGC 
GGCTGCCAGA TCATGACCGT CGATCGGCCC AAGCCATTCA CCTTCATCAT CCGCGGCCTG 
CAATGGACCA CGGTAATCGA AAGGACATTT GCCGTCGAAT CAGAATTAGA GCGCCAGCAG 
TGGACGGAGG CCATTCGCAA CGTATCCAGC CGGCTCATAG ATGTGGGTGA GGTGGCTATG 
ACGCCATCTG AACAGACAGA CATGACAGAC GTGGACATGG CCACCATTGC CGAGGACGAG 
CTTTCCGAGC AGTTCTCCGT ACAGGGAACG ACTTGTAATA GCAGCGGCGT TAAGAAAGTG 
ACTTTGGAGA ATTTTGAGTT CCTCAAGGTG CTCGGCAAGG GCACCTTTGG TAAGGTTATT 
TTGTGTCGCG AGAAGGCTAC CGCCAAGCTG TACGCGATCA AAATTCTTAA AAAGGAAGTT 
ATTATCCAAA AGGACGAGGT GGCCCATACC CTGACCGAGA GTCGTGTGCT CAAGTCCACA 
AATCATCCGT TCCTCATTTC ACTCAAATAT TCGTTTCAAA CCAACGACCG CCTGTGTTTT 
GTGATGCAGT ACGTGAACGG TGGCGAGCTC TTTTGGCATT TAAGCCACGA ACGCATATTT 
ACTGAGGATC GAACACGTTT CTATGGGGCA GAGATCATTT CTGCTCTGGG CTATCTGCAT 
TCACAGGGCA TAATTTATCG CGACTTAAAG CTGGAAAATC TTTTGCTGGA CAAAGATGGT 
CACATAAAGG TCGCAGACTT CGGTCTGTGC AAGGAGGACA TCACCTACGG CCGCACAACG 
AAAACCTTCT GCGGTACACC GGAATACCTA GCTCCGGAAG TATTAGATGA CAATGATTAT 
GGCCAAGCGG TGGACTGGTG GGGCACTGGC GTGGTCATGT ACGAGATGAT CTGTGGTCGT 
CTTCCGTTTT ACAATCGTGA TCATGATGTG CTCTTTACAT TGATTTTGGT GGAGGAGGTA 
AAATTCCCGC GTAATATCAC AGATGAGGCG AAAAATCTGT TAGCAGGCCT TCTAGCTAAG 
GATCCCAAAA AGCGTTTGGG AGGTGGAAAG GATGATGTCA AGGAGATACA AGCACATCCA 
TTCTTTGCGA GTATTAACTG GACAGATCTA GTGTTGAAAA AAATACCGCC GCCTTTCAAG 
CCTCAGGTGA CATCCGATAC GGATACAAGG TACTTCGACA AGGAGTTTAC GGGAGAGAGT 
GTGGAGTTGA CGCCGCCGGA TCCCACTGGC CCGTTGGGCT CCATAGCCGA AGAGCCGCTT 
TTCCCGCAGT TCAGCTACCA AGGAGACATG GCCTCCACGT TGGGCACCTC GTCGCACATT 
AGTACTTCCA CAAGTCTCGC ATCGATGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akt1 ( NM_169705.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732113.3 Drosophila melanogaster Akt1, transcript variant C (Akt1), mRNA.

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CloneID	OFa05088
Clone ID Related Accession (Same CDS sequence)	NM_169705.2
Accession Version	NM_169705.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Akt1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169705.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ACGAATTACC TACCGTTTGT TCTTCAGCGG CGATCGACTG TGGTTGCCAG CGCCCCAGCG 
CCAGGATCTG CCTCTAGGAT CCCGGAATCG CCCACCACCA CAGGGAGCAA CATCATTAAC 
ATCATCTACA GCCAATCCAC CCATCCAAAC AGCAGCCCCA CCAGCGGCAG TGCAGAGAAA 
TTCAGCTGGC AGCAATCCTG GCCAAGCAGG ACCTCCGCGG CCCCTACACA CGATTCCGGA 
ACCATGTCAA TAAACACAAC TTTCGACCTC AGCTCGCCCA GCGTTACATC GGGTCATGCG 
CTTACGGAAC AGACTCAGGT CGTAAAGGAG GGGTGGCTGA TGAAGAGGGG CGAGCACATA 
AAGAACTGGC GCCAGCGCTA CTTCGTGCTC CACTCCGATG GAAGACTGAT GGGTTACCGC 
AGCAAGCCGG CAGATAGTGC CAGCACGCCA TCGGACTTCC TGCTCAACAA CTTTACGGTG 
CGCGGCTGCC AGATCATGAC CGTCGATCGG CCCAAGCCAT TCACCTTCAT CATCCGCGGC 
CTGCAATGGA CCACGGTAAT CGAAAGGACA TTTGCCGTCG AATCAGAATT AGAGCGCCAG 
CAGTGGACGG AGGCCATTCG CAACGTATCC AGCCGGCTCA TAGATGTGGG TGAGGTGGCT 
ATGACGCCAT CTGAACAGAC AGACATGACA GACGTGGACA TGGCCACCAT TGCCGAGGAC 
GAGCTTTCCG AGCAGTTCTC CGTACAGGGA ACGACTTGTA ATAGCAGCGG CGTTAAGAAA 
GTGACTTTGG AGAATTTTGA GTTCCTCAAG GTGCTCGGCA AGGGCACCTT TGGTAAGGTT 
ATTTTGTGTC GCGAGAAGGC TACCGCCAAG CTGTACGCGA TCAAAATTCT TAAAAAGGAA 
GTTATTATCC AAAAGGACGA GGTGGCCCAT ACCCTGACCG AGAGTCGTGT GCTCAAGTCC 
ACAAATCATC CGTTCCTCAT TTCACTCAAA TATTCGTTTC AAACCAACGA CCGCCTGTGT 
TTTGTGATGC AGTACGTGAA CGGTGGCGAG CTCTTTTGGC ATTTAAGCCA CGAACGCATA 
TTTACTGAGG ATCGAACACG TTTCTATGGG GCAGAGATCA TTTCTGCTCT GGGCTATCTG 
CATTCACAGG GCATAATTTA TCGCGACTTA AAGCTGGAAA ATCTTTTGCT GGACAAAGAT 
GGTCACATAA AGGTCGCAGA CTTCGGTCTG TGCAAGGAGG ACATCACCTA CGGCCGCACA 
ACGAAAACCT TCTGCGGTAC ACCGGAATAC CTAGCTCCGG AAGTATTAGA TGACAATGAT 
TATGGCCAAG CGGTGGACTG GTGGGGCACT GGCGTGGTCA TGTACGAGAT GATCTGTGGT 
CGTCTTCCGT TTTACAATCG TGATCATGAT GTGCTCTTTA CATTGATTTT GGTGGAGGAG 
GTAAAATTCC CGCGTAATAT CACAGATGAG GCGAAAAATC TGTTAGCAGG CCTTCTAGCT 
AAGGATCCCA AAAAGCGTTT GGGAGGTGGA AAGGATGATG TCAAGGAGAT ACAAGCACAT 
CCATTCTTTG CGAGTATTAA CTGGACAGAT CTAGTGTTGA AAAAAATACC GCCGCCTTTC 
AAGCCTCAGG TGACATCCGA TACGGATACA AGGTACTTCG ACAAGGAGTT TACGGGAGAG 
AGTGTGGAGT TGACGCCGCC GGATCCCACT GGCCCGTTGG GCTCCATAGC CGAAGAGCCG 
CTTTTCCCGC AGTTCAGCTA CCAAGGAGAC ATGGCCTCCA CGTTGGGCAC CTCGTCGCAC 
ATTAGTACTT CCACAAGTCT CGCATCGATG CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732113.3
CDS	413..2248
Translation	TNYLPFVLQRRSTVVASAPAPGSASRIPESPTTTGSNIINIIYSQSTHPNSSPTSGSAEKFSWQQSWPSRTSAAPTHDSGTMSINTTFDLSSPSVTSGHALTEQTQVVKEGWLMKRGEHIKNWRQRYFVLHSDGRLMGYRSKPADSASTPSDFLLNNFTVRGCQIMTVDRPKPFTFIIRGLQWTTVIERTFAVESELERQQWTEAIRNVSSRLIDVGEVAMTPSEQTDMTDVDMATIAEDELSEQFSVQGTTCNSSGVKKVTLENFEFLKVLGKGTFGKVILCREKATAKLYAIKILKKEVIIQKDEVAHTLTESRVLKSTNHPFLISLKYSFQTNDRLCFVMQYVNGGELFWHLSHERIFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFGLCKEDITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKRLGGGKDDVKEIQAHPFFASINWTDLVLKKIPPPFKPQVTSDTDTRYFDKEFTGESVELTPPDPTGPLGSIAEEPLFPQFSYQGDMASTLGTSSHISTSTSLASMQ

Target ORF information:

RefSeq Version	NM_169705.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Akt1, transcript variant C (Akt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169705.2

ORF Insert Sequence:

ACGAATTACC TACCGTTTGT TCTTCAGCGG CGATCGACTG TGGTTGCCAG CGCCCCAGCG 
CCAGGATCTG CCTCTAGGAT CCCGGAATCG CCCACCACCA CAGGGAGCAA CATCATTAAC 
ATCATCTACA GCCAATCCAC CCATCCAAAC AGCAGCCCCA CCAGCGGCAG TGCAGAGAAA 
TTCAGCTGGC AGCAATCCTG GCCAAGCAGG ACCTCCGCGG CCCCTACACA CGATTCCGGA 
ACCATGTCAA TAAACACAAC TTTCGACCTC AGCTCGCCCA GCGTTACATC GGGTCATGCG 
CTTACGGAAC AGACTCAGGT CGTAAAGGAG GGGTGGCTGA TGAAGAGGGG CGAGCACATA 
AAGAACTGGC GCCAGCGCTA CTTCGTGCTC CACTCCGATG GAAGACTGAT GGGTTACCGC 
AGCAAGCCGG CAGATAGTGC CAGCACGCCA TCGGACTTCC TGCTCAACAA CTTTACGGTG 
CGCGGCTGCC AGATCATGAC CGTCGATCGG CCCAAGCCAT TCACCTTCAT CATCCGCGGC 
CTGCAATGGA CCACGGTAAT CGAAAGGACA TTTGCCGTCG AATCAGAATT AGAGCGCCAG 
CAGTGGACGG AGGCCATTCG CAACGTATCC AGCCGGCTCA TAGATGTGGG TGAGGTGGCT 
ATGACGCCAT CTGAACAGAC AGACATGACA GACGTGGACA TGGCCACCAT TGCCGAGGAC 
GAGCTTTCCG AGCAGTTCTC CGTACAGGGA ACGACTTGTA ATAGCAGCGG CGTTAAGAAA 
GTGACTTTGG AGAATTTTGA GTTCCTCAAG GTGCTCGGCA AGGGCACCTT TGGTAAGGTT 
ATTTTGTGTC GCGAGAAGGC TACCGCCAAG CTGTACGCGA TCAAAATTCT TAAAAAGGAA 
GTTATTATCC AAAAGGACGA GGTGGCCCAT ACCCTGACCG AGAGTCGTGT GCTCAAGTCC 
ACAAATCATC CGTTCCTCAT TTCACTCAAA TATTCGTTTC AAACCAACGA CCGCCTGTGT 
TTTGTGATGC AGTACGTGAA CGGTGGCGAG CTCTTTTGGC ATTTAAGCCA CGAACGCATA 
TTTACTGAGG ATCGAACACG TTTCTATGGG GCAGAGATCA TTTCTGCTCT GGGCTATCTG 
CATTCACAGG GCATAATTTA TCGCGACTTA AAGCTGGAAA ATCTTTTGCT GGACAAAGAT 
GGTCACATAA AGGTCGCAGA CTTCGGTCTG TGCAAGGAGG ACATCACCTA CGGCCGCACA 
ACGAAAACCT TCTGCGGTAC ACCGGAATAC CTAGCTCCGG AAGTATTAGA TGACAATGAT 
TATGGCCAAG CGGTGGACTG GTGGGGCACT GGCGTGGTCA TGTACGAGAT GATCTGTGGT 
CGTCTTCCGT TTTACAATCG TGATCATGAT GTGCTCTTTA CATTGATTTT GGTGGAGGAG 
GTAAAATTCC CGCGTAATAT CACAGATGAG GCGAAAAATC TGTTAGCAGG CCTTCTAGCT 
AAGGATCCCA AAAAGCGTTT GGGAGGTGGA AAGGATGATG TCAAGGAGAT ACAAGCACAT 
CCATTCTTTG CGAGTATTAA CTGGACAGAT CTAGTGTTGA AAAAAATACC GCCGCCTTTC 
AAGCCTCAGG TGACATCCGA TACGGATACA AGGTACTTCG ACAAGGAGTT TACGGGAGAG 
AGTGTGGAGT TGACGCCGCC GGATCCCACT GGCCCGTTGG GCTCCATAGC CGAAGAGCCG 
CTTTTCCCGC AGTTCAGCTA CCAAGGAGAC ATGGCCTCCA CGTTGGGCAC CTCGTCGCAC 
ATTAGTACTT CCACAAGTCT CGCATCGATG CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Akt1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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