Hel89B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hel89B
Entrez Gene ID	41943
Full Name	Helicase 89B
Synonyms	89B,BTAF1,CG4261,Dmel\CG4261,HEL89B,Helicase89B,Mot1
General protein information	Preferred Names Helicase 89B Names CG4261 gene product from transcript CG4261-RB 89B helicase CG4261-PB CG4261-PC Hel89B-PB Hel89B-PC Helicase89B helicase 89B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89A11-89A11

mRNA and Protein(s)

mRNA	Protein	Name
NM_169693.2	NP_732097.2	helicase 89B, isoform B
NM_001300422.1	NP_001287351.1	helicase 89B, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	BTAF1	NP_003963.1
Danio rerio (zebrafish)	btaf1	XP_005169589.1
Saccharomyces cerevisiae (baker's yeast)	MOT1	NP_015243.1
Gallus gallus (chicken)	BTAF1	XP_421689.3
Eremothecium gossypii	AGOS_AEL256C	NP_984605.2
Magnaporthe oryzae (rice blast fungus)	MGG_04989	XP_003712489.1
Pan troglodytes (chimpanzee)	BTAF1	XP_003312799.1
Canis lupus familiaris (dog)	BTAF1	XP_003640032.1
Bos taurus (cattle)	BTAF1	XP_003587915.2
Arabidopsis thaliana (thale cress)	RGD3	NP_190996.3
Rattus norvegicus (Norway rat)	Btaf1	NP_001178846.1
Drosophila melanogaster (fruit fly)	Hel89B	NP_732097.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001820	XP_321235.5
Schizosaccharomyces pombe (fission yeast)	mot1	NP_596080.2
	BTAF1	XP_002805795.1
Mus musculus (house mouse)	Btaf1	NP_001074175.1
Kluyveromyces lactis	KLLA0E23717g	XP_455023.1
Neurospora crassa	NCU07556	XP_958601.1
Xenopus tropicalis (tropical clawed frog)	btaf1	XP_002939139.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TBP activates TATA-dependent transcription and represses DPE-dependent transcription, whereas Mot1 and NC2 block TBP function and thus repress TATA-dependent transcription and activate DPE-dependent transcription.
Title: TBP, Mot1, and NC2 establish a regulatory circuit that controls DPE-dependent versus TATA-dependent transcription.

The following Hel89B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hel89B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05070	NM_169693.2 Latest version!	Drosophila melanogaster helicase 89B, transcript variant B (Hel89B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa05070	NM_001300422.1 Latest version!	Drosophila melanogaster helicase 89B, transcript variant C (Hel89B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hel89B ( NM_169693.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732097.2 Drosophila melanogaster helicase 89B, transcript variant B (Hel89B), mRNA.

Price & Availability↑

CloneID	OFa05070
Clone ID Related Accession (Same CDS sequence)	NM_169693.2 , NM_001300422.1
Accession Version	NM_169693.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	helicase 89B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_169693.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACATCTA GACTTGATCG GCTGTTCATT CTACTGGAGA CGGGCTCTAC AGCGGCCACT 
CGTCAGGCGG CCGCCAAGCA GATTGGTGAG GTTCAAAAGC TTTATCCACA CGAGCTGCAC 
GCTCTGCTGA ACAGATTGGT GGGCTACCTG CACAGTACAT CCTGGGAGAC ACGGATAGCT 
GCAGCCCAGA CCGTTGAGGC CATATTAAAG CAAGTACCGC CCTGGCAGCC CGAATTCCAG 
GTGCTGCCCA AGAAGGAGCG TTGCGCTACT AATACTGCCA CAGCTGCAGC GGCAGCAGAA 
GAGGACAGTT GCCAGTCAAG TGGTTCCAAC TCGACAACGG CCAGCGAGCG ACTACTTAGC 
TTTGATGAGT TCGATCTGCA GCAAATCCTC CACAAAGGTG CCAGACTCAT CGGCTCCGAG 
GGCAACGAGT TTGATGTGCA GGAGGAGCAA CCGGCTAGTG GAGGCGGGGA GGAGGACAGA 
AGCCTAGCCA GTCGTCTAAG CCGCCAGCGT GCGGTGCTGA ACGACAAACT GGGTCTGACA 
ACGGCCGCCA AACTGGGCGT TAATCTTACC GATATGATAA CAGACGAGGA TGTGGCACTC 
AACAGGAGTG GCAGCACTTA CAATGTCAAC GCAGAAAAGC TTCCAGTTGA GCACTTACTC 
AACATAAAGA CAGCGGCGAA CGGATCTGGT CAAGCTCCAC TTAGTTGCCG TGAGATGAAC 
AGGGCCAAGC GCAAGGCTCG TCAAAATACA AGCGGATGTT CTGTCACTGC AGTTACGCCA 
ACTTGCAGCC GGAGGAACTC CAACAGCAAT CACAGTACTG GCAGCAATCA CAGCAGTAAT 
GGCACCACTG GTGCTGCAAT AGAAGAACCC GAAAAGAAGA AACTTAAGTC AACGGATCGG 
CAGGAAATCT TCTATAGTTT AAATGCTGCT GTGCCAGATG CTACAGGCAC TTGGGTGGAT 
GCCGTCTCCT GGCCACTGGA GAACTTCTGC TCACGTCTCT TCATTGATCT CTTTCACGCC 
AAATGGGAGG TGCGCCATGG GGCGGCAACA GCTCTAAGGG AACTGATTAA TCAGCACGCT 
CAAGGAGCGG GAAAGGCAGT GCTACAGACT CGCGAACAGA TGGATGAAGC ACATAGCCGA 
TGGCTAGAGG ATGCTGCCCT GCGCCTGCTC TGTGTGCTTT GTCTGGATCG GTTTGGTGAC 
TTTGTCTCGG ATCAGGTTGT GGCACCGGTT CGCGAGACCT GCGCCCAAGT GCTGGGTACG 
TTGGTCAAGG AGATGGAGGC GTCCAAGGTT CAACGAATCG TGAACATTCT GCTTCAGCTC 
ATCCAGCACA AGAACGAGTG GGATGTGCGG CATGGTGGAC TACTAGGCCT GAAGTATGTG 
TTTGTGGTGC GTGAGGAGCT GCTGCCCATC TATTTGCCCC AGTCAATTTC AAACATTCTC 
ATCGGCCTGC TGGACGCTGT GGATGATGTC GGAGCGGTGG CTGCCGCAAC CCTGATTCCA 
GTTTCTTCCT GGCTGCCGAA GCTATTAAAT CCGGCGCAAG TATCTTCCAT CGTAAAAATG 
TTGTGGGACC TACTATTGGA TCAAGATGAA CTCACGTCTG CCTGCAACAG TTTCATGGGG 
CTGTTAGCGG CCATTCTATG CCTACCAAAC GCTGCTCGTT GGGTAGAGAT GGAGCCGATG 
GCCATTTTGG TACCGCGCCT GTGGCCTTTT CTTTCCCATA GCACTAGTTC GGTACGAAGA 
TCAACGCTGA AGACGCTTAT GACCCTGACG AGTGCGGATA GGGTCAAATC CGAGCCCAAA 
GACGAAAATC AAGTTAAAGA GGAAGCGGAT AAGGAAGAAA TGAAACTTAA CTTTGGAGTC 
AGCGACTGGA AGTGGGAATT GCTGCAGCAG GCCCTGCGTC ACATATATCA ACGCATCCTA 
GTGGAGCCGC AGGCGGATAT CCAGGCATTA GCCCGTCAGG TGTGGTCCAA TCTTATCCAG 
CATGCAGATT TGGGTGCGTT GTTGCATGCT GCCTGTCCAT TCGTGTCGTC CTGGATATGC 
CTTTCAATGC AGCCACCTAG ATTGGCTTTT GATGCAGGGG TCCTGATCAG AGCTGGTGGA 
GATGTAAGCA CGCCAGGAAG CACCTCCCGA AAACGAACGC CCCGCATAGG AGACGAATTG 
GGCGGTAACA TTCTAGCGCA ATCAAACGCC TCATTGAAAT TATACCTTGG TGGTAGCGAA 
GCCACGCCTT TGGATGTCCG GGATGCCAAT TTTATGCGAG CCCGGATTTC CTCATCTCGG 
GCACTGGGTG CACTTTCTCA TTACCTGGTT CAGCCAGCAC CGGGAGTGGT GTATACCCCA 
CAAACGGAGA GTCCCACAGA CTGTTATACA AAAGTACTAT TGGGTCATTT AAATGCCCAC 
TCTGCTGTCC AGCGAATCGT CTGCGGGCTG ATCATTGCGT TTTGGGCGCT AGAAGACGAG 
CCAGTTCGCC CAGGACCGCC CAATCTGCAG GAAAAGTTAC ACCAATGTGT GAGCGAGTAC 
GTCTACTACG ATGAGGTAGC CATCTCACTG ACCCGACTAC TACAAGAGGC ACAAGACTTT 
ATTGCTACGC TGAAGCAGAA CAAAATCCCG ATTAACGATT TCAATAATGC CAAGATCCTT 
ACTCTAGATC AAATCGAGGC AGTGGCAACT ACGCTAAGCG AAAATCTGCG TTCTTATCCC 
CTCAAGCCGA AAGTTTTAGA TATGCTAGAG GAGCGTCGAC GCAGCCTTCA GGCCTCCTAC 
CAGCAGACAA CCAGTGAGCA AAGCGCCTAT CATGTCAGTG CTCAGGCAGC ACTCGCTGGC 
GCCATCTGCG CTCTTCACTG CCTGCCAGAT AAGCTGAATC CGGTGGTCAA GCCATTAATG 
GAGTCCATCA AGCGGGAGCA GTGCCTCCAG CTGCAGCAGC TGTCTGCCGA GTTCCTGGTC 
CACCTTATGG ATCAGGTCTG TGATAGAAAT CCTAGTCCAA ATAGCAAGAT TTTAAACAAC 
CTTTGTACGC TGTTAAGGAG CGATCCCGAA TTCACTCCCA AACTGGTAAT GCCACTAGAA 
ACCCTCAAAC AGACGCCAGT TTCGTCTGAG GTTATTAATA ATTGTGTTTA CTATGGTATC 
CTAACACTGG CACTTCAACA GACAGTGACA ACCACTAACA CGCGAAGCGG TGCGGGCGGT 
GCAACAACTC CCACTGCTAC TACGGCCCCC AGGGGCCCTG GGCGACCGCC CACTGGCGAA 
ATATTGTCCG CAACCAACGC CACTGCTCAC ATTGAGCTTA AGGCGCAGCA AGCCAAGGAG 
GCGGAGGCGA AGCAATGCCG CATACAGCGC CTGGGAGCGG CATGTGCCAT TGAAAAGCTG 
TGTAGGATAT TTGGAGAGCA AATCATTGAA AAGGTGGCTG TTTTCCAACA TCTGATGTTT 
GGCAAGGTGG AGCAGTTCGT AAAAGAAGCG TACGACTGGC GATTGGGCAG CCTTCTTCCA 
GATCTGGGAG TGTGCAATGA GCTTGTCAGC TCTATGCAGC TTATTGAGAC AGCAGCCCCC 
CATCTGCATG TTGCACTGCA TCCTCAGATG TTTGCCCTTC TGCCCTCCCT TGGCTTTATC 
GTTTGCCATC CTCTTAAGGC AGTCCGACAC ATGGCCGCTC GTTGCATCGC CGTGCTGGCC 
GAGATCGATG CTTGCCAGAC TATGCAGTTT GTGGTTCACG ATCTGTTACC GCTGCTTGGG 
AAGATTGAGC AACTTATAGA GCGACAAGGA GCCATAGAGG CTATCGAACG CGTGGTCAGC 
AGGCTGCAGA TAAAAGTGGT GCCCTACATC GTGCTGCTGG TGGTGCCTCT ACTGGGCGCA 
ATGAGCGATC CGGATGAGTC AGTGCGATTG CTTTCCACGC ACTGCTTTGC AAATCTAGTC 
CAGTTAATGC CTCTGGACGG AAAGACGGAG CAATTAAAAA GCGATCCGCT GCAGGCGCGC 
AAAACGCGTG ACCGTGAGTT TCTCGACTAT CTTTTCAATC CAAAGAGCAT TCCCAATTAT 
AAGGTTCCGG TACCAATAAG TGTGGAGCTG CGCTGCTATC AACAAGCCGG CATCAACTGG 
CTGTGGTTCC TGAACAAATA CAATCTACAC GGCATTCTTT GCGACGATAT GGGGTTGGGC 
AAAACTCTGC AAACCATATG CATCCTTGCC GGAGACCACA TGCACCGTCA GACAGCCAAT 
TTGGCGAATC TGCCTAGTCT CGTCATTTGC CCACCAACTT TAACCGGTCA TTGGGTGTAC 
GAGGTGGAGA AATTCCTTGA CCAGGGATCC GTTCTGCGTC CCTTACACTA CTACGGATTC 
CCAGTGGGAA GAGAGAAGCT AAGGAGTGAT ATCGGAACAA AGTGTAACCT GGTTGTGGCA 
TCGTACGACA CTGTGCGCAA GGACATCGAC TTCTTCAGCG GAATACACTT TAACTATTGC 
GTTTTGGATG AAGGCCATAT TATTAAGAAC GGAAAGACAA AGAGCTCTAA GGCAATAAAG 
CGATTAAAGG CAAACCATAG ACTGATCCTC TCCGGCACTC CCATCCAGAA CAATGTGCTC 
GAGTTGTGGT CCTTGTTTGA CTTTTTAATG CCCGGCTTCC TTGGCACTGA GAAGCAGTTC 
GTGCAACGCT TTTCACGTCC CATTCTGTCA TCTCGGGATG CAAAGAGCTC TGCCAAGGAG 
CAGGAAGCTG GAGTGCTGGC CATGGAGGCG CTGCATAGAC AAGTTCTGCC CTTCCTGCTG 
CGCCGTGTTA AGGAGGATGT GCTGAAGGAC CTACCGCCAA AGATCACCCA GGACTTACTT 
TGCGAACTGA GTCCGCTCCA GCTTCGACTG TATGAAGATT TTAGCAATAA ACACCTGAAA 
GACTGTTTAG ACAAACTGGG CGACTCGTCG TCGGCATCCA TGGTGACGGA GAATCTCAGC 
GCCAAGACAC ACATTTTCCA AGCGCTTCGA TACCTGCAGA ACGTATGCAA CCATCCCAAG 
CTGGTGTTGC GTCAGTCTGA GGAGCTCACG AAGGTGACCA GTCAGTTGGC GTTGTCAAAC 
AGCTCATTGG ACGACATTGA GCACTCTGCA AAGCTGCCCG CCTTGAAGCA ACTTCTCCTA 
GACTGCGGCA TTGGTGTGCA GACGGAGTCG GTGAGCCAGC ATCGTGCCCT AATCTTCTGC 
CAGCTGAAGG CGATGCTGGA CATTGTGGAA CAGGATCTAT TGCGCCGCCA TCTGCCCAGC 
GTCACATATC TGCGGCTGGA CGGCAGTGTA CCCGCCTCAC AGCGACAGGA TATAGTCAAT 
AACTTCAACA GCGATCCCAG CATCGATGTC CTGCTCCTGA CCACTATGGT TGGCGGCCTG 
GGTCTAAATC TAACAGGCGC CGATACGGTC ATCTTTGTCG AGCACGACTG GAATCCAATG 
AAGGATCTGC AGGCTATGGA CCGCGCCCAC CGAATCGGCC AAAAGAAAGT AGTAAATGTT 
TACCGGCTGA TCACACGCAA CTCGCTGGAG GAAAAGATCA TGGGGCTGCA AAAGTTTAAG 
ATACTCACCG CCAACACTGT GGTGAGTGCG GAGAACGCTT CGCTGCAGAC AATGGGCACC 
TCACAGATCT TTGACCTCTT CAACGGAGGG AAAGACAAGG GAGCAGAATC TGGATCATCA 
GCGGTGCAAG GAACAGCATC GGGCGGCATG TCCATGAACA CCATTATCGA GAACTTGCCG 
GAACTCTGGT CCGAGCATCA GTACGAGGAA GAGTACGACT TGGGCAACTT TGTGCAGGCA 
CTGAAAAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732097.2
CDS	584..6355
Translation	MTSRLDRLFILLETGSTAATRQAAAKQIGEVQKLYPHELHALLNRLVGYLHSTSWETRIAAAQTVEAILKQVPPWQPEFQVLPKKERCATNTATAAAAAEEDSCQSSGSNSTTASERLLSFDEFDLQQILHKGARLIGSEGNEFDVQEEQPASGGGEEDRSLASRLSRQRAVLNDKLGLTTAAKLGVNLTDMITDEDVALNRSGSTYNVNAEKLPVEHLLNIKTAANGSGQAPLSCREMNRAKRKARQNTSGCSVTAVTPTCSRRNSNSNHSTGSNHSSNGTTGAAIEEPEKKKLKSTDRQEIFYSLNAAVPDATGTWVDAVSWPLENFCSRLFIDLFHAKWEVRHGAATALRELINQHAQGAGKAVLQTREQMDEAHSRWLEDAALRLLCVLCLDRFGDFVSDQVVAPVRETCAQVLGTLVKEMEASKVQRIVNILLQLIQHKNEWDVRHGGLLGLKYVFVVREELLPIYLPQSISNILIGLLDAVDDVGAVAAATLIPVSSWLPKLLNPAQVSSIVKMLWDLLLDQDELTSACNSFMGLLAAILCLPNAARWVEMEPMAILVPRLWPFLSHSTSSVRRSTLKTLMTLTSADRVKSEPKDENQVKEEADKEEMKLNFGVSDWKWELLQQALRHIYQRILVEPQADIQALARQVWSNLIQHADLGALLHAACPFVSSWICLSMQPPRLAFDAGVLIRAGGDVSTPGSTSRKRTPRIGDELGGNILAQSNASLKLYLGGSEATPLDVRDANFMRARISSSRALGALSHYLVQPAPGVVYTPQTESPTDCYTKVLLGHLNAHSAVQRIVCGLIIAFWALEDEPVRPGPPNLQEKLHQCVSEYVYYDEVAISLTRLLQEAQDFIATLKQNKIPINDFNNAKILTLDQIEAVATTLSENLRSYPLKPKVLDMLEERRRSLQASYQQTTSEQSAYHVSAQAALAGAICALHCLPDKLNPVVKPLMESIKREQCLQLQQLSAEFLVHLMDQVCDRNPSPNSKILNNLCTLLRSDPEFTPKLVMPLETLKQTPVSSEVINNCVYYGILTLALQQTVTTTNTRSGAGGATTPTATTAPRGPGRPPTGEILSATNATAHIELKAQQAKEAEAKQCRIQRLGAACAIEKLCRIFGEQIIEKVAVFQHLMFGKVEQFVKEAYDWRLGSLLPDLGVCNELVSSMQLIETAAPHLHVALHPQMFALLPSLGFIVCHPLKAVRHMAARCIAVLAEIDACQTMQFVVHDLLPLLGKIEQLIERQGAIEAIERVVSRLQIKVVPYIVLLVVPLLGAMSDPDESVRLLSTHCFANLVQLMPLDGKTEQLKSDPLQARKTRDREFLDYLFNPKSIPNYKVPVPISVELRCYQQAGINWLWFLNKYNLHGILCDDMGLGKTLQTICILAGDHMHRQTANLANLPSLVICPPTLTGHWVYEVEKFLDQGSVLRPLHYYGFPVGREKLRSDIGTKCNLVVASYDTVRKDIDFFSGIHFNYCVLDEGHIIKNGKTKSSKAIKRLKANHRLILSGTPIQNNVLELWSLFDFLMPGFLGTEKQFVQRFSRPILSSRDAKSSAKEQEAGVLAMEALHRQVLPFLLRRVKEDVLKDLPPKITQDLLCELSPLQLRLYEDFSNKHLKDCLDKLGDSSSASMVTENLSAKTHIFQALRYLQNVCNHPKLVLRQSEELTKVTSQLALSNSSLDDIEHSAKLPALKQLLLDCGIGVQTESVSQHRALIFCQLKAMLDIVEQDLLRRHLPSVTYLRLDGSVPASQRQDIVNNFNSDPSIDVLLLTTMVGGLGLNLTGADTVIFVEHDWNPMKDLQAMDRAHRIGQKKVVNVYRLITRNSLEEKIMGLQKFKILTANTVVSAENASLQTMGTSQIFDLFNGGKDKGAESGSSAVQGTASGGMSMNTIIENLPELWSEHQYEEEYDLGNFVQALKK

Target ORF information:

RefSeq Version	NM_169693.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster helicase 89B, transcript variant B (Hel89B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169693.2

ORF Insert Sequence:

ATGACATCTA GACTTGATCG GCTGTTCATT CTACTGGAGA CGGGCTCTAC AGCGGCCACT 
CGTCAGGCGG CCGCCAAGCA GATTGGTGAG GTTCAAAAGC TTTATCCACA CGAGCTGCAC 
GCTCTGCTGA ACAGATTGGT GGGCTACCTG CACAGTACAT CCTGGGAGAC ACGGATAGCT 
GCAGCCCAGA CCGTTGAGGC CATATTAAAG CAAGTACCGC CCTGGCAGCC CGAATTCCAG 
GTGCTGCCCA AGAAGGAGCG TTGCGCTACT AATACTGCCA CAGCTGCAGC GGCAGCAGAA 
GAGGACAGTT GCCAGTCAAG TGGTTCCAAC TCGACAACGG CCAGCGAGCG ACTACTTAGC 
TTTGATGAGT TCGATCTGCA GCAAATCCTC CACAAAGGTG CCAGACTCAT CGGCTCCGAG 
GGCAACGAGT TTGATGTGCA GGAGGAGCAA CCGGCTAGTG GAGGCGGGGA GGAGGACAGA 
AGCCTAGCCA GTCGTCTAAG CCGCCAGCGT GCGGTGCTGA ACGACAAACT GGGTCTGACA 
ACGGCCGCCA AACTGGGCGT TAATCTTACC GATATGATAA CAGACGAGGA TGTGGCACTC 
AACAGGAGTG GCAGCACTTA CAATGTCAAC GCAGAAAAGC TTCCAGTTGA GCACTTACTC 
AACATAAAGA CAGCGGCGAA CGGATCTGGT CAAGCTCCAC TTAGTTGCCG TGAGATGAAC 
AGGGCCAAGC GCAAGGCTCG TCAAAATACA AGCGGATGTT CTGTCACTGC AGTTACGCCA 
ACTTGCAGCC GGAGGAACTC CAACAGCAAT CACAGTACTG GCAGCAATCA CAGCAGTAAT 
GGCACCACTG GTGCTGCAAT AGAAGAACCC GAAAAGAAGA AACTTAAGTC AACGGATCGG 
CAGGAAATCT TCTATAGTTT AAATGCTGCT GTGCCAGATG CTACAGGCAC TTGGGTGGAT 
GCCGTCTCCT GGCCACTGGA GAACTTCTGC TCACGTCTCT TCATTGATCT CTTTCACGCC 
AAATGGGAGG TGCGCCATGG GGCGGCAACA GCTCTAAGGG AACTGATTAA TCAGCACGCT 
CAAGGAGCGG GAAAGGCAGT GCTACAGACT CGCGAACAGA TGGATGAAGC ACATAGCCGA 
TGGCTAGAGG ATGCTGCCCT GCGCCTGCTC TGTGTGCTTT GTCTGGATCG GTTTGGTGAC 
TTTGTCTCGG ATCAGGTTGT GGCACCGGTT CGCGAGACCT GCGCCCAAGT GCTGGGTACG 
TTGGTCAAGG AGATGGAGGC GTCCAAGGTT CAACGAATCG TGAACATTCT GCTTCAGCTC 
ATCCAGCACA AGAACGAGTG GGATGTGCGG CATGGTGGAC TACTAGGCCT GAAGTATGTG 
TTTGTGGTGC GTGAGGAGCT GCTGCCCATC TATTTGCCCC AGTCAATTTC AAACATTCTC 
ATCGGCCTGC TGGACGCTGT GGATGATGTC GGAGCGGTGG CTGCCGCAAC CCTGATTCCA 
GTTTCTTCCT GGCTGCCGAA GCTATTAAAT CCGGCGCAAG TATCTTCCAT CGTAAAAATG 
TTGTGGGACC TACTATTGGA TCAAGATGAA CTCACGTCTG CCTGCAACAG TTTCATGGGG 
CTGTTAGCGG CCATTCTATG CCTACCAAAC GCTGCTCGTT GGGTAGAGAT GGAGCCGATG 
GCCATTTTGG TACCGCGCCT GTGGCCTTTT CTTTCCCATA GCACTAGTTC GGTACGAAGA 
TCAACGCTGA AGACGCTTAT GACCCTGACG AGTGCGGATA GGGTCAAATC CGAGCCCAAA 
GACGAAAATC AAGTTAAAGA GGAAGCGGAT AAGGAAGAAA TGAAACTTAA CTTTGGAGTC 
AGCGACTGGA AGTGGGAATT GCTGCAGCAG GCCCTGCGTC ACATATATCA ACGCATCCTA 
GTGGAGCCGC AGGCGGATAT CCAGGCATTA GCCCGTCAGG TGTGGTCCAA TCTTATCCAG 
CATGCAGATT TGGGTGCGTT GTTGCATGCT GCCTGTCCAT TCGTGTCGTC CTGGATATGC 
CTTTCAATGC AGCCACCTAG ATTGGCTTTT GATGCAGGGG TCCTGATCAG AGCTGGTGGA 
GATGTAAGCA CGCCAGGAAG CACCTCCCGA AAACGAACGC CCCGCATAGG AGACGAATTG 
GGCGGTAACA TTCTAGCGCA ATCAAACGCC TCATTGAAAT TATACCTTGG TGGTAGCGAA 
GCCACGCCTT TGGATGTCCG GGATGCCAAT TTTATGCGAG CCCGGATTTC CTCATCTCGG 
GCACTGGGTG CACTTTCTCA TTACCTGGTT CAGCCAGCAC CGGGAGTGGT GTATACCCCA 
CAAACGGAGA GTCCCACAGA CTGTTATACA AAAGTACTAT TGGGTCATTT AAATGCCCAC 
TCTGCTGTCC AGCGAATCGT CTGCGGGCTG ATCATTGCGT TTTGGGCGCT AGAAGACGAG 
CCAGTTCGCC CAGGACCGCC CAATCTGCAG GAAAAGTTAC ACCAATGTGT GAGCGAGTAC 
GTCTACTACG ATGAGGTAGC CATCTCACTG ACCCGACTAC TACAAGAGGC ACAAGACTTT 
ATTGCTACGC TGAAGCAGAA CAAAATCCCG ATTAACGATT TCAATAATGC CAAGATCCTT 
ACTCTAGATC AAATCGAGGC AGTGGCAACT ACGCTAAGCG AAAATCTGCG TTCTTATCCC 
CTCAAGCCGA AAGTTTTAGA TATGCTAGAG GAGCGTCGAC GCAGCCTTCA GGCCTCCTAC 
CAGCAGACAA CCAGTGAGCA AAGCGCCTAT CATGTCAGTG CTCAGGCAGC ACTCGCTGGC 
GCCATCTGCG CTCTTCACTG CCTGCCAGAT AAGCTGAATC CGGTGGTCAA GCCATTAATG 
GAGTCCATCA AGCGGGAGCA GTGCCTCCAG CTGCAGCAGC TGTCTGCCGA GTTCCTGGTC 
CACCTTATGG ATCAGGTCTG TGATAGAAAT CCTAGTCCAA ATAGCAAGAT TTTAAACAAC 
CTTTGTACGC TGTTAAGGAG CGATCCCGAA TTCACTCCCA AACTGGTAAT GCCACTAGAA 
ACCCTCAAAC AGACGCCAGT TTCGTCTGAG GTTATTAATA ATTGTGTTTA CTATGGTATC 
CTAACACTGG CACTTCAACA GACAGTGACA ACCACTAACA CGCGAAGCGG TGCGGGCGGT 
GCAACAACTC CCACTGCTAC TACGGCCCCC AGGGGCCCTG GGCGACCGCC CACTGGCGAA 
ATATTGTCCG CAACCAACGC CACTGCTCAC ATTGAGCTTA AGGCGCAGCA AGCCAAGGAG 
GCGGAGGCGA AGCAATGCCG CATACAGCGC CTGGGAGCGG CATGTGCCAT TGAAAAGCTG 
TGTAGGATAT TTGGAGAGCA AATCATTGAA AAGGTGGCTG TTTTCCAACA TCTGATGTTT 
GGCAAGGTGG AGCAGTTCGT AAAAGAAGCG TACGACTGGC GATTGGGCAG CCTTCTTCCA 
GATCTGGGAG TGTGCAATGA GCTTGTCAGC TCTATGCAGC TTATTGAGAC AGCAGCCCCC 
CATCTGCATG TTGCACTGCA TCCTCAGATG TTTGCCCTTC TGCCCTCCCT TGGCTTTATC 
GTTTGCCATC CTCTTAAGGC AGTCCGACAC ATGGCCGCTC GTTGCATCGC CGTGCTGGCC 
GAGATCGATG CTTGCCAGAC TATGCAGTTT GTGGTTCACG ATCTGTTACC GCTGCTTGGG 
AAGATTGAGC AACTTATAGA GCGACAAGGA GCCATAGAGG CTATCGAACG CGTGGTCAGC 
AGGCTGCAGA TAAAAGTGGT GCCCTACATC GTGCTGCTGG TGGTGCCTCT ACTGGGCGCA 
ATGAGCGATC CGGATGAGTC AGTGCGATTG CTTTCCACGC ACTGCTTTGC AAATCTAGTC 
CAGTTAATGC CTCTGGACGG AAAGACGGAG CAATTAAAAA GCGATCCGCT GCAGGCGCGC 
AAAACGCGTG ACCGTGAGTT TCTCGACTAT CTTTTCAATC CAAAGAGCAT TCCCAATTAT 
AAGGTTCCGG TACCAATAAG TGTGGAGCTG CGCTGCTATC AACAAGCCGG CATCAACTGG 
CTGTGGTTCC TGAACAAATA CAATCTACAC GGCATTCTTT GCGACGATAT GGGGTTGGGC 
AAAACTCTGC AAACCATATG CATCCTTGCC GGAGACCACA TGCACCGTCA GACAGCCAAT 
TTGGCGAATC TGCCTAGTCT CGTCATTTGC CCACCAACTT TAACCGGTCA TTGGGTGTAC 
GAGGTGGAGA AATTCCTTGA CCAGGGATCC GTTCTGCGTC CCTTACACTA CTACGGATTC 
CCAGTGGGAA GAGAGAAGCT AAGGAGTGAT ATCGGAACAA AGTGTAACCT GGTTGTGGCA 
TCGTACGACA CTGTGCGCAA GGACATCGAC TTCTTCAGCG GAATACACTT TAACTATTGC 
GTTTTGGATG AAGGCCATAT TATTAAGAAC GGAAAGACAA AGAGCTCTAA GGCAATAAAG 
CGATTAAAGG CAAACCATAG ACTGATCCTC TCCGGCACTC CCATCCAGAA CAATGTGCTC 
GAGTTGTGGT CCTTGTTTGA CTTTTTAATG CCCGGCTTCC TTGGCACTGA GAAGCAGTTC 
GTGCAACGCT TTTCACGTCC CATTCTGTCA TCTCGGGATG CAAAGAGCTC TGCCAAGGAG 
CAGGAAGCTG GAGTGCTGGC CATGGAGGCG CTGCATAGAC AAGTTCTGCC CTTCCTGCTG 
CGCCGTGTTA AGGAGGATGT GCTGAAGGAC CTACCGCCAA AGATCACCCA GGACTTACTT 
TGCGAACTGA GTCCGCTCCA GCTTCGACTG TATGAAGATT TTAGCAATAA ACACCTGAAA 
GACTGTTTAG ACAAACTGGG CGACTCGTCG TCGGCATCCA TGGTGACGGA GAATCTCAGC 
GCCAAGACAC ACATTTTCCA AGCGCTTCGA TACCTGCAGA ACGTATGCAA CCATCCCAAG 
CTGGTGTTGC GTCAGTCTGA GGAGCTCACG AAGGTGACCA GTCAGTTGGC GTTGTCAAAC 
AGCTCATTGG ACGACATTGA GCACTCTGCA AAGCTGCCCG CCTTGAAGCA ACTTCTCCTA 
GACTGCGGCA TTGGTGTGCA GACGGAGTCG GTGAGCCAGC ATCGTGCCCT AATCTTCTGC 
CAGCTGAAGG CGATGCTGGA CATTGTGGAA CAGGATCTAT TGCGCCGCCA TCTGCCCAGC 
GTCACATATC TGCGGCTGGA CGGCAGTGTA CCCGCCTCAC AGCGACAGGA TATAGTCAAT 
AACTTCAACA GCGATCCCAG CATCGATGTC CTGCTCCTGA CCACTATGGT TGGCGGCCTG 
GGTCTAAATC TAACAGGCGC CGATACGGTC ATCTTTGTCG AGCACGACTG GAATCCAATG 
AAGGATCTGC AGGCTATGGA CCGCGCCCAC CGAATCGGCC AAAAGAAAGT AGTAAATGTT 
TACCGGCTGA TCACACGCAA CTCGCTGGAG GAAAAGATCA TGGGGCTGCA AAAGTTTAAG 
ATACTCACCG CCAACACTGT GGTGAGTGCG GAGAACGCTT CGCTGCAGAC AATGGGCACC 
TCACAGATCT TTGACCTCTT CAACGGAGGG AAAGACAAGG GAGCAGAATC TGGATCATCA 
GCGGTGCAAG GAACAGCATC GGGCGGCATG TCCATGAACA CCATTATCGA GAACTTGCCG 
GAACTCTGGT CCGAGCATCA GTACGAGGAA GAGTACGACT TGGGCAACTT TGTGCAGGCA 
CTGAAAAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hel89B ( NM_001300422.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287351.1 Drosophila melanogaster helicase 89B, transcript variant C (Hel89B), mRNA.

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CloneID	OFa05070
Clone ID Related Accession (Same CDS sequence)	NM_169693.2 , NM_001300422.1
Accession Version	NM_001300422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	helicase 89B, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACATCTA GACTTGATCG GCTGTTCATT CTACTGGAGA CGGGCTCTAC AGCGGCCACT 
CGTCAGGCGG CCGCCAAGCA GATTGGTGAG GTTCAAAAGC TTTATCCACA CGAGCTGCAC 
GCTCTGCTGA ACAGATTGGT GGGCTACCTG CACAGTACAT CCTGGGAGAC ACGGATAGCT 
GCAGCCCAGA CCGTTGAGGC CATATTAAAG CAAGTACCGC CCTGGCAGCC CGAATTCCAG 
GTGCTGCCCA AGAAGGAGCG TTGCGCTACT AATACTGCCA CAGCTGCAGC GGCAGCAGAA 
GAGGACAGTT GCCAGTCAAG TGGTTCCAAC TCGACAACGG CCAGCGAGCG ACTACTTAGC 
TTTGATGAGT TCGATCTGCA GCAAATCCTC CACAAAGGTG CCAGACTCAT CGGCTCCGAG 
GGCAACGAGT TTGATGTGCA GGAGGAGCAA CCGGCTAGTG GAGGCGGGGA GGAGGACAGA 
AGCCTAGCCA GTCGTCTAAG CCGCCAGCGT GCGGTGCTGA ACGACAAACT GGGTCTGACA 
ACGGCCGCCA AACTGGGCGT TAATCTTACC GATATGATAA CAGACGAGGA TGTGGCACTC 
AACAGGAGTG GCAGCACTTA CAATGTCAAC GCAGAAAAGC TTCCAGTTGA GCACTTACTC 
AACATAAAGA CAGCGGCGAA CGGATCTGGT CAAGCTCCAC TTAGTTGCCG TGAGATGAAC 
AGGGCCAAGC GCAAGGCTCG TCAAAATACA AGCGGATGTT CTGTCACTGC AGTTACGCCA 
ACTTGCAGCC GGAGGAACTC CAACAGCAAT CACAGTACTG GCAGCAATCA CAGCAGTAAT 
GGCACCACTG GTGCTGCAAT AGAAGAACCC GAAAAGAAGA AACTTAAGTC AACGGATCGG 
CAGGAAATCT TCTATAGTTT AAATGCTGCT GTGCCAGATG CTACAGGCAC TTGGGTGGAT 
GCCGTCTCCT GGCCACTGGA GAACTTCTGC TCACGTCTCT TCATTGATCT CTTTCACGCC 
AAATGGGAGG TGCGCCATGG GGCGGCAACA GCTCTAAGGG AACTGATTAA TCAGCACGCT 
CAAGGAGCGG GAAAGGCAGT GCTACAGACT CGCGAACAGA TGGATGAAGC ACATAGCCGA 
TGGCTAGAGG ATGCTGCCCT GCGCCTGCTC TGTGTGCTTT GTCTGGATCG GTTTGGTGAC 
TTTGTCTCGG ATCAGGTTGT GGCACCGGTT CGCGAGACCT GCGCCCAAGT GCTGGGTACG 
TTGGTCAAGG AGATGGAGGC GTCCAAGGTT CAACGAATCG TGAACATTCT GCTTCAGCTC 
ATCCAGCACA AGAACGAGTG GGATGTGCGG CATGGTGGAC TACTAGGCCT GAAGTATGTG 
TTTGTGGTGC GTGAGGAGCT GCTGCCCATC TATTTGCCCC AGTCAATTTC AAACATTCTC 
ATCGGCCTGC TGGACGCTGT GGATGATGTC GGAGCGGTGG CTGCCGCAAC CCTGATTCCA 
GTTTCTTCCT GGCTGCCGAA GCTATTAAAT CCGGCGCAAG TATCTTCCAT CGTAAAAATG 
TTGTGGGACC TACTATTGGA TCAAGATGAA CTCACGTCTG CCTGCAACAG TTTCATGGGG 
CTGTTAGCGG CCATTCTATG CCTACCAAAC GCTGCTCGTT GGGTAGAGAT GGAGCCGATG 
GCCATTTTGG TACCGCGCCT GTGGCCTTTT CTTTCCCATA GCACTAGTTC GGTACGAAGA 
TCAACGCTGA AGACGCTTAT GACCCTGACG AGTGCGGATA GGGTCAAATC CGAGCCCAAA 
GACGAAAATC AAGTTAAAGA GGAAGCGGAT AAGGAAGAAA TGAAACTTAA CTTTGGAGTC 
AGCGACTGGA AGTGGGAATT GCTGCAGCAG GCCCTGCGTC ACATATATCA ACGCATCCTA 
GTGGAGCCGC AGGCGGATAT CCAGGCATTA GCCCGTCAGG TGTGGTCCAA TCTTATCCAG 
CATGCAGATT TGGGTGCGTT GTTGCATGCT GCCTGTCCAT TCGTGTCGTC CTGGATATGC 
CTTTCAATGC AGCCACCTAG ATTGGCTTTT GATGCAGGGG TCCTGATCAG AGCTGGTGGA 
GATGTAAGCA CGCCAGGAAG CACCTCCCGA AAACGAACGC CCCGCATAGG AGACGAATTG 
GGCGGTAACA TTCTAGCGCA ATCAAACGCC TCATTGAAAT TATACCTTGG TGGTAGCGAA 
GCCACGCCTT TGGATGTCCG GGATGCCAAT TTTATGCGAG CCCGGATTTC CTCATCTCGG 
GCACTGGGTG CACTTTCTCA TTACCTGGTT CAGCCAGCAC CGGGAGTGGT GTATACCCCA 
CAAACGGAGA GTCCCACAGA CTGTTATACA AAAGTACTAT TGGGTCATTT AAATGCCCAC 
TCTGCTGTCC AGCGAATCGT CTGCGGGCTG ATCATTGCGT TTTGGGCGCT AGAAGACGAG 
CCAGTTCGCC CAGGACCGCC CAATCTGCAG GAAAAGTTAC ACCAATGTGT GAGCGAGTAC 
GTCTACTACG ATGAGGTAGC CATCTCACTG ACCCGACTAC TACAAGAGGC ACAAGACTTT 
ATTGCTACGC TGAAGCAGAA CAAAATCCCG ATTAACGATT TCAATAATGC CAAGATCCTT 
ACTCTAGATC AAATCGAGGC AGTGGCAACT ACGCTAAGCG AAAATCTGCG TTCTTATCCC 
CTCAAGCCGA AAGTTTTAGA TATGCTAGAG GAGCGTCGAC GCAGCCTTCA GGCCTCCTAC 
CAGCAGACAA CCAGTGAGCA AAGCGCCTAT CATGTCAGTG CTCAGGCAGC ACTCGCTGGC 
GCCATCTGCG CTCTTCACTG CCTGCCAGAT AAGCTGAATC CGGTGGTCAA GCCATTAATG 
GAGTCCATCA AGCGGGAGCA GTGCCTCCAG CTGCAGCAGC TGTCTGCCGA GTTCCTGGTC 
CACCTTATGG ATCAGGTCTG TGATAGAAAT CCTAGTCCAA ATAGCAAGAT TTTAAACAAC 
CTTTGTACGC TGTTAAGGAG CGATCCCGAA TTCACTCCCA AACTGGTAAT GCCACTAGAA 
ACCCTCAAAC AGACGCCAGT TTCGTCTGAG GTTATTAATA ATTGTGTTTA CTATGGTATC 
CTAACACTGG CACTTCAACA GACAGTGACA ACCACTAACA CGCGAAGCGG TGCGGGCGGT 
GCAACAACTC CCACTGCTAC TACGGCCCCC AGGGGCCCTG GGCGACCGCC CACTGGCGAA 
ATATTGTCCG CAACCAACGC CACTGCTCAC ATTGAGCTTA AGGCGCAGCA AGCCAAGGAG 
GCGGAGGCGA AGCAATGCCG CATACAGCGC CTGGGAGCGG CATGTGCCAT TGAAAAGCTG 
TGTAGGATAT TTGGAGAGCA AATCATTGAA AAGGTGGCTG TTTTCCAACA TCTGATGTTT 
GGCAAGGTGG AGCAGTTCGT AAAAGAAGCG TACGACTGGC GATTGGGCAG CCTTCTTCCA 
GATCTGGGAG TGTGCAATGA GCTTGTCAGC TCTATGCAGC TTATTGAGAC AGCAGCCCCC 
CATCTGCATG TTGCACTGCA TCCTCAGATG TTTGCCCTTC TGCCCTCCCT TGGCTTTATC 
GTTTGCCATC CTCTTAAGGC AGTCCGACAC ATGGCCGCTC GTTGCATCGC CGTGCTGGCC 
GAGATCGATG CTTGCCAGAC TATGCAGTTT GTGGTTCACG ATCTGTTACC GCTGCTTGGG 
AAGATTGAGC AACTTATAGA GCGACAAGGA GCCATAGAGG CTATCGAACG CGTGGTCAGC 
AGGCTGCAGA TAAAAGTGGT GCCCTACATC GTGCTGCTGG TGGTGCCTCT ACTGGGCGCA 
ATGAGCGATC CGGATGAGTC AGTGCGATTG CTTTCCACGC ACTGCTTTGC AAATCTAGTC 
CAGTTAATGC CTCTGGACGG AAAGACGGAG CAATTAAAAA GCGATCCGCT GCAGGCGCGC 
AAAACGCGTG ACCGTGAGTT TCTCGACTAT CTTTTCAATC CAAAGAGCAT TCCCAATTAT 
AAGGTTCCGG TACCAATAAG TGTGGAGCTG CGCTGCTATC AACAAGCCGG CATCAACTGG 
CTGTGGTTCC TGAACAAATA CAATCTACAC GGCATTCTTT GCGACGATAT GGGGTTGGGC 
AAAACTCTGC AAACCATATG CATCCTTGCC GGAGACCACA TGCACCGTCA GACAGCCAAT 
TTGGCGAATC TGCCTAGTCT CGTCATTTGC CCACCAACTT TAACCGGTCA TTGGGTGTAC 
GAGGTGGAGA AATTCCTTGA CCAGGGATCC GTTCTGCGTC CCTTACACTA CTACGGATTC 
CCAGTGGGAA GAGAGAAGCT AAGGAGTGAT ATCGGAACAA AGTGTAACCT GGTTGTGGCA 
TCGTACGACA CTGTGCGCAA GGACATCGAC TTCTTCAGCG GAATACACTT TAACTATTGC 
GTTTTGGATG AAGGCCATAT TATTAAGAAC GGAAAGACAA AGAGCTCTAA GGCAATAAAG 
CGATTAAAGG CAAACCATAG ACTGATCCTC TCCGGCACTC CCATCCAGAA CAATGTGCTC 
GAGTTGTGGT CCTTGTTTGA CTTTTTAATG CCCGGCTTCC TTGGCACTGA GAAGCAGTTC 
GTGCAACGCT TTTCACGTCC CATTCTGTCA TCTCGGGATG CAAAGAGCTC TGCCAAGGAG 
CAGGAAGCTG GAGTGCTGGC CATGGAGGCG CTGCATAGAC AAGTTCTGCC CTTCCTGCTG 
CGCCGTGTTA AGGAGGATGT GCTGAAGGAC CTACCGCCAA AGATCACCCA GGACTTACTT 
TGCGAACTGA GTCCGCTCCA GCTTCGACTG TATGAAGATT TTAGCAATAA ACACCTGAAA 
GACTGTTTAG ACAAACTGGG CGACTCGTCG TCGGCATCCA TGGTGACGGA GAATCTCAGC 
GCCAAGACAC ACATTTTCCA AGCGCTTCGA TACCTGCAGA ACGTATGCAA CCATCCCAAG 
CTGGTGTTGC GTCAGTCTGA GGAGCTCACG AAGGTGACCA GTCAGTTGGC GTTGTCAAAC 
AGCTCATTGG ACGACATTGA GCACTCTGCA AAGCTGCCCG CCTTGAAGCA ACTTCTCCTA 
GACTGCGGCA TTGGTGTGCA GACGGAGTCG GTGAGCCAGC ATCGTGCCCT AATCTTCTGC 
CAGCTGAAGG CGATGCTGGA CATTGTGGAA CAGGATCTAT TGCGCCGCCA TCTGCCCAGC 
GTCACATATC TGCGGCTGGA CGGCAGTGTA CCCGCCTCAC AGCGACAGGA TATAGTCAAT 
AACTTCAACA GCGATCCCAG CATCGATGTC CTGCTCCTGA CCACTATGGT TGGCGGCCTG 
GGTCTAAATC TAACAGGCGC CGATACGGTC ATCTTTGTCG AGCACGACTG GAATCCAATG 
AAGGATCTGC AGGCTATGGA CCGCGCCCAC CGAATCGGCC AAAAGAAAGT AGTAAATGTT 
TACCGGCTGA TCACACGCAA CTCGCTGGAG GAAAAGATCA TGGGGCTGCA AAAGTTTAAG 
ATACTCACCG CCAACACTGT GGTGAGTGCG GAGAACGCTT CGCTGCAGAC AATGGGCACC 
TCACAGATCT TTGACCTCTT CAACGGAGGG AAAGACAAGG GAGCAGAATC TGGATCATCA 
GCGGTGCAAG GAACAGCATC GGGCGGCATG TCCATGAACA CCATTATCGA GAACTTGCCG 
GAACTCTGGT CCGAGCATCA GTACGAGGAA GAGTACGACT TGGGCAACTT TGTGCAGGCA 
CTGAAAAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287351.1
CDS	157..5928
Translation	MTSRLDRLFILLETGSTAATRQAAAKQIGEVQKLYPHELHALLNRLVGYLHSTSWETRIAAAQTVEAILKQVPPWQPEFQVLPKKERCATNTATAAAAAEEDSCQSSGSNSTTASERLLSFDEFDLQQILHKGARLIGSEGNEFDVQEEQPASGGGEEDRSLASRLSRQRAVLNDKLGLTTAAKLGVNLTDMITDEDVALNRSGSTYNVNAEKLPVEHLLNIKTAANGSGQAPLSCREMNRAKRKARQNTSGCSVTAVTPTCSRRNSNSNHSTGSNHSSNGTTGAAIEEPEKKKLKSTDRQEIFYSLNAAVPDATGTWVDAVSWPLENFCSRLFIDLFHAKWEVRHGAATALRELINQHAQGAGKAVLQTREQMDEAHSRWLEDAALRLLCVLCLDRFGDFVSDQVVAPVRETCAQVLGTLVKEMEASKVQRIVNILLQLIQHKNEWDVRHGGLLGLKYVFVVREELLPIYLPQSISNILIGLLDAVDDVGAVAAATLIPVSSWLPKLLNPAQVSSIVKMLWDLLLDQDELTSACNSFMGLLAAILCLPNAARWVEMEPMAILVPRLWPFLSHSTSSVRRSTLKTLMTLTSADRVKSEPKDENQVKEEADKEEMKLNFGVSDWKWELLQQALRHIYQRILVEPQADIQALARQVWSNLIQHADLGALLHAACPFVSSWICLSMQPPRLAFDAGVLIRAGGDVSTPGSTSRKRTPRIGDELGGNILAQSNASLKLYLGGSEATPLDVRDANFMRARISSSRALGALSHYLVQPAPGVVYTPQTESPTDCYTKVLLGHLNAHSAVQRIVCGLIIAFWALEDEPVRPGPPNLQEKLHQCVSEYVYYDEVAISLTRLLQEAQDFIATLKQNKIPINDFNNAKILTLDQIEAVATTLSENLRSYPLKPKVLDMLEERRRSLQASYQQTTSEQSAYHVSAQAALAGAICALHCLPDKLNPVVKPLMESIKREQCLQLQQLSAEFLVHLMDQVCDRNPSPNSKILNNLCTLLRSDPEFTPKLVMPLETLKQTPVSSEVINNCVYYGILTLALQQTVTTTNTRSGAGGATTPTATTAPRGPGRPPTGEILSATNATAHIELKAQQAKEAEAKQCRIQRLGAACAIEKLCRIFGEQIIEKVAVFQHLMFGKVEQFVKEAYDWRLGSLLPDLGVCNELVSSMQLIETAAPHLHVALHPQMFALLPSLGFIVCHPLKAVRHMAARCIAVLAEIDACQTMQFVVHDLLPLLGKIEQLIERQGAIEAIERVVSRLQIKVVPYIVLLVVPLLGAMSDPDESVRLLSTHCFANLVQLMPLDGKTEQLKSDPLQARKTRDREFLDYLFNPKSIPNYKVPVPISVELRCYQQAGINWLWFLNKYNLHGILCDDMGLGKTLQTICILAGDHMHRQTANLANLPSLVICPPTLTGHWVYEVEKFLDQGSVLRPLHYYGFPVGREKLRSDIGTKCNLVVASYDTVRKDIDFFSGIHFNYCVLDEGHIIKNGKTKSSKAIKRLKANHRLILSGTPIQNNVLELWSLFDFLMPGFLGTEKQFVQRFSRPILSSRDAKSSAKEQEAGVLAMEALHRQVLPFLLRRVKEDVLKDLPPKITQDLLCELSPLQLRLYEDFSNKHLKDCLDKLGDSSSASMVTENLSAKTHIFQALRYLQNVCNHPKLVLRQSEELTKVTSQLALSNSSLDDIEHSAKLPALKQLLLDCGIGVQTESVSQHRALIFCQLKAMLDIVEQDLLRRHLPSVTYLRLDGSVPASQRQDIVNNFNSDPSIDVLLLTTMVGGLGLNLTGADTVIFVEHDWNPMKDLQAMDRAHRIGQKKVVNVYRLITRNSLEEKIMGLQKFKILTANTVVSAENASLQTMGTSQIFDLFNGGKDKGAESGSSAVQGTASGGMSMNTIIENLPELWSEHQYEEEYDLGNFVQALKK

Target ORF information:

RefSeq Version	NM_001300422.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster helicase 89B, transcript variant C (Hel89B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300422.1

ORF Insert Sequence:

ATGACATCTA GACTTGATCG GCTGTTCATT CTACTGGAGA CGGGCTCTAC AGCGGCCACT 
CGTCAGGCGG CCGCCAAGCA GATTGGTGAG GTTCAAAAGC TTTATCCACA CGAGCTGCAC 
GCTCTGCTGA ACAGATTGGT GGGCTACCTG CACAGTACAT CCTGGGAGAC ACGGATAGCT 
GCAGCCCAGA CCGTTGAGGC CATATTAAAG CAAGTACCGC CCTGGCAGCC CGAATTCCAG 
GTGCTGCCCA AGAAGGAGCG TTGCGCTACT AATACTGCCA CAGCTGCAGC GGCAGCAGAA 
GAGGACAGTT GCCAGTCAAG TGGTTCCAAC TCGACAACGG CCAGCGAGCG ACTACTTAGC 
TTTGATGAGT TCGATCTGCA GCAAATCCTC CACAAAGGTG CCAGACTCAT CGGCTCCGAG 
GGCAACGAGT TTGATGTGCA GGAGGAGCAA CCGGCTAGTG GAGGCGGGGA GGAGGACAGA 
AGCCTAGCCA GTCGTCTAAG CCGCCAGCGT GCGGTGCTGA ACGACAAACT GGGTCTGACA 
ACGGCCGCCA AACTGGGCGT TAATCTTACC GATATGATAA CAGACGAGGA TGTGGCACTC 
AACAGGAGTG GCAGCACTTA CAATGTCAAC GCAGAAAAGC TTCCAGTTGA GCACTTACTC 
AACATAAAGA CAGCGGCGAA CGGATCTGGT CAAGCTCCAC TTAGTTGCCG TGAGATGAAC 
AGGGCCAAGC GCAAGGCTCG TCAAAATACA AGCGGATGTT CTGTCACTGC AGTTACGCCA 
ACTTGCAGCC GGAGGAACTC CAACAGCAAT CACAGTACTG GCAGCAATCA CAGCAGTAAT 
GGCACCACTG GTGCTGCAAT AGAAGAACCC GAAAAGAAGA AACTTAAGTC AACGGATCGG 
CAGGAAATCT TCTATAGTTT AAATGCTGCT GTGCCAGATG CTACAGGCAC TTGGGTGGAT 
GCCGTCTCCT GGCCACTGGA GAACTTCTGC TCACGTCTCT TCATTGATCT CTTTCACGCC 
AAATGGGAGG TGCGCCATGG GGCGGCAACA GCTCTAAGGG AACTGATTAA TCAGCACGCT 
CAAGGAGCGG GAAAGGCAGT GCTACAGACT CGCGAACAGA TGGATGAAGC ACATAGCCGA 
TGGCTAGAGG ATGCTGCCCT GCGCCTGCTC TGTGTGCTTT GTCTGGATCG GTTTGGTGAC 
TTTGTCTCGG ATCAGGTTGT GGCACCGGTT CGCGAGACCT GCGCCCAAGT GCTGGGTACG 
TTGGTCAAGG AGATGGAGGC GTCCAAGGTT CAACGAATCG TGAACATTCT GCTTCAGCTC 
ATCCAGCACA AGAACGAGTG GGATGTGCGG CATGGTGGAC TACTAGGCCT GAAGTATGTG 
TTTGTGGTGC GTGAGGAGCT GCTGCCCATC TATTTGCCCC AGTCAATTTC AAACATTCTC 
ATCGGCCTGC TGGACGCTGT GGATGATGTC GGAGCGGTGG CTGCCGCAAC CCTGATTCCA 
GTTTCTTCCT GGCTGCCGAA GCTATTAAAT CCGGCGCAAG TATCTTCCAT CGTAAAAATG 
TTGTGGGACC TACTATTGGA TCAAGATGAA CTCACGTCTG CCTGCAACAG TTTCATGGGG 
CTGTTAGCGG CCATTCTATG CCTACCAAAC GCTGCTCGTT GGGTAGAGAT GGAGCCGATG 
GCCATTTTGG TACCGCGCCT GTGGCCTTTT CTTTCCCATA GCACTAGTTC GGTACGAAGA 
TCAACGCTGA AGACGCTTAT GACCCTGACG AGTGCGGATA GGGTCAAATC CGAGCCCAAA 
GACGAAAATC AAGTTAAAGA GGAAGCGGAT AAGGAAGAAA TGAAACTTAA CTTTGGAGTC 
AGCGACTGGA AGTGGGAATT GCTGCAGCAG GCCCTGCGTC ACATATATCA ACGCATCCTA 
GTGGAGCCGC AGGCGGATAT CCAGGCATTA GCCCGTCAGG TGTGGTCCAA TCTTATCCAG 
CATGCAGATT TGGGTGCGTT GTTGCATGCT GCCTGTCCAT TCGTGTCGTC CTGGATATGC 
CTTTCAATGC AGCCACCTAG ATTGGCTTTT GATGCAGGGG TCCTGATCAG AGCTGGTGGA 
GATGTAAGCA CGCCAGGAAG CACCTCCCGA AAACGAACGC CCCGCATAGG AGACGAATTG 
GGCGGTAACA TTCTAGCGCA ATCAAACGCC TCATTGAAAT TATACCTTGG TGGTAGCGAA 
GCCACGCCTT TGGATGTCCG GGATGCCAAT TTTATGCGAG CCCGGATTTC CTCATCTCGG 
GCACTGGGTG CACTTTCTCA TTACCTGGTT CAGCCAGCAC CGGGAGTGGT GTATACCCCA 
CAAACGGAGA GTCCCACAGA CTGTTATACA AAAGTACTAT TGGGTCATTT AAATGCCCAC 
TCTGCTGTCC AGCGAATCGT CTGCGGGCTG ATCATTGCGT TTTGGGCGCT AGAAGACGAG 
CCAGTTCGCC CAGGACCGCC CAATCTGCAG GAAAAGTTAC ACCAATGTGT GAGCGAGTAC 
GTCTACTACG ATGAGGTAGC CATCTCACTG ACCCGACTAC TACAAGAGGC ACAAGACTTT 
ATTGCTACGC TGAAGCAGAA CAAAATCCCG ATTAACGATT TCAATAATGC CAAGATCCTT 
ACTCTAGATC AAATCGAGGC AGTGGCAACT ACGCTAAGCG AAAATCTGCG TTCTTATCCC 
CTCAAGCCGA AAGTTTTAGA TATGCTAGAG GAGCGTCGAC GCAGCCTTCA GGCCTCCTAC 
CAGCAGACAA CCAGTGAGCA AAGCGCCTAT CATGTCAGTG CTCAGGCAGC ACTCGCTGGC 
GCCATCTGCG CTCTTCACTG CCTGCCAGAT AAGCTGAATC CGGTGGTCAA GCCATTAATG 
GAGTCCATCA AGCGGGAGCA GTGCCTCCAG CTGCAGCAGC TGTCTGCCGA GTTCCTGGTC 
CACCTTATGG ATCAGGTCTG TGATAGAAAT CCTAGTCCAA ATAGCAAGAT TTTAAACAAC 
CTTTGTACGC TGTTAAGGAG CGATCCCGAA TTCACTCCCA AACTGGTAAT GCCACTAGAA 
ACCCTCAAAC AGACGCCAGT TTCGTCTGAG GTTATTAATA ATTGTGTTTA CTATGGTATC 
CTAACACTGG CACTTCAACA GACAGTGACA ACCACTAACA CGCGAAGCGG TGCGGGCGGT 
GCAACAACTC CCACTGCTAC TACGGCCCCC AGGGGCCCTG GGCGACCGCC CACTGGCGAA 
ATATTGTCCG CAACCAACGC CACTGCTCAC ATTGAGCTTA AGGCGCAGCA AGCCAAGGAG 
GCGGAGGCGA AGCAATGCCG CATACAGCGC CTGGGAGCGG CATGTGCCAT TGAAAAGCTG 
TGTAGGATAT TTGGAGAGCA AATCATTGAA AAGGTGGCTG TTTTCCAACA TCTGATGTTT 
GGCAAGGTGG AGCAGTTCGT AAAAGAAGCG TACGACTGGC GATTGGGCAG CCTTCTTCCA 
GATCTGGGAG TGTGCAATGA GCTTGTCAGC TCTATGCAGC TTATTGAGAC AGCAGCCCCC 
CATCTGCATG TTGCACTGCA TCCTCAGATG TTTGCCCTTC TGCCCTCCCT TGGCTTTATC 
GTTTGCCATC CTCTTAAGGC AGTCCGACAC ATGGCCGCTC GTTGCATCGC CGTGCTGGCC 
GAGATCGATG CTTGCCAGAC TATGCAGTTT GTGGTTCACG ATCTGTTACC GCTGCTTGGG 
AAGATTGAGC AACTTATAGA GCGACAAGGA GCCATAGAGG CTATCGAACG CGTGGTCAGC 
AGGCTGCAGA TAAAAGTGGT GCCCTACATC GTGCTGCTGG TGGTGCCTCT ACTGGGCGCA 
ATGAGCGATC CGGATGAGTC AGTGCGATTG CTTTCCACGC ACTGCTTTGC AAATCTAGTC 
CAGTTAATGC CTCTGGACGG AAAGACGGAG CAATTAAAAA GCGATCCGCT GCAGGCGCGC 
AAAACGCGTG ACCGTGAGTT TCTCGACTAT CTTTTCAATC CAAAGAGCAT TCCCAATTAT 
AAGGTTCCGG TACCAATAAG TGTGGAGCTG CGCTGCTATC AACAAGCCGG CATCAACTGG 
CTGTGGTTCC TGAACAAATA CAATCTACAC GGCATTCTTT GCGACGATAT GGGGTTGGGC 
AAAACTCTGC AAACCATATG CATCCTTGCC GGAGACCACA TGCACCGTCA GACAGCCAAT 
TTGGCGAATC TGCCTAGTCT CGTCATTTGC CCACCAACTT TAACCGGTCA TTGGGTGTAC 
GAGGTGGAGA AATTCCTTGA CCAGGGATCC GTTCTGCGTC CCTTACACTA CTACGGATTC 
CCAGTGGGAA GAGAGAAGCT AAGGAGTGAT ATCGGAACAA AGTGTAACCT GGTTGTGGCA 
TCGTACGACA CTGTGCGCAA GGACATCGAC TTCTTCAGCG GAATACACTT TAACTATTGC 
GTTTTGGATG AAGGCCATAT TATTAAGAAC GGAAAGACAA AGAGCTCTAA GGCAATAAAG 
CGATTAAAGG CAAACCATAG ACTGATCCTC TCCGGCACTC CCATCCAGAA CAATGTGCTC 
GAGTTGTGGT CCTTGTTTGA CTTTTTAATG CCCGGCTTCC TTGGCACTGA GAAGCAGTTC 
GTGCAACGCT TTTCACGTCC CATTCTGTCA TCTCGGGATG CAAAGAGCTC TGCCAAGGAG 
CAGGAAGCTG GAGTGCTGGC CATGGAGGCG CTGCATAGAC AAGTTCTGCC CTTCCTGCTG 
CGCCGTGTTA AGGAGGATGT GCTGAAGGAC CTACCGCCAA AGATCACCCA GGACTTACTT 
TGCGAACTGA GTCCGCTCCA GCTTCGACTG TATGAAGATT TTAGCAATAA ACACCTGAAA 
GACTGTTTAG ACAAACTGGG CGACTCGTCG TCGGCATCCA TGGTGACGGA GAATCTCAGC 
GCCAAGACAC ACATTTTCCA AGCGCTTCGA TACCTGCAGA ACGTATGCAA CCATCCCAAG 
CTGGTGTTGC GTCAGTCTGA GGAGCTCACG AAGGTGACCA GTCAGTTGGC GTTGTCAAAC 
AGCTCATTGG ACGACATTGA GCACTCTGCA AAGCTGCCCG CCTTGAAGCA ACTTCTCCTA 
GACTGCGGCA TTGGTGTGCA GACGGAGTCG GTGAGCCAGC ATCGTGCCCT AATCTTCTGC 
CAGCTGAAGG CGATGCTGGA CATTGTGGAA CAGGATCTAT TGCGCCGCCA TCTGCCCAGC 
GTCACATATC TGCGGCTGGA CGGCAGTGTA CCCGCCTCAC AGCGACAGGA TATAGTCAAT 
AACTTCAACA GCGATCCCAG CATCGATGTC CTGCTCCTGA CCACTATGGT TGGCGGCCTG 
GGTCTAAATC TAACAGGCGC CGATACGGTC ATCTTTGTCG AGCACGACTG GAATCCAATG 
AAGGATCTGC AGGCTATGGA CCGCGCCCAC CGAATCGGCC AAAAGAAAGT AGTAAATGTT 
TACCGGCTGA TCACACGCAA CTCGCTGGAG GAAAAGATCA TGGGGCTGCA AAAGTTTAAG 
ATACTCACCG CCAACACTGT GGTGAGTGCG GAGAACGCTT CGCTGCAGAC AATGGGCACC 
TCACAGATCT TTGACCTCTT CAACGGAGGG AAAGACAAGG GAGCAGAATC TGGATCATCA 
GCGGTGCAAG GAACAGCATC GGGCGGCATG TCCATGAACA CCATTATCGA GAACTTGCCG 
GAACTCTGGT CCGAGCATCA GTACGAGGAA GAGTACGACT TGGGCAACTT TGTGCAGGCA 
CTGAAAAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hel89B cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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