alpha-Man-IIb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	alpha-Man-IIb
Entrez Gene ID	41913
Full Name	alpha-Mannosidase class II b
Synonyms	CG4606,DM-GII.2,Dmel\CG4606,GM,GTD#24/1,Man'ase,ManIIb,alpha-M-II,alpha-Man-II b,dGMIIb
General protein information	Preferred Names alpha-Mannosidase class II b Names CG4606 gene product from transcript CG4606-RA CG4606-PA CG4606-PB CG4606-PC alpha mannosidase IIb alpha-D-mannosidase alpha-Man-IIb-PA alpha-Man-IIb-PB alpha-Man-IIb-PC alpha-mannosidase alpha-mannosidase 2 alpha-mannosidase-IIb golgi alpha-mannosidase IIb mannosidase II
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89A5-89A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_142237.3	NP_650494.2	alpha-Mannosidase class II b, isoform A
NM_001275685.1	NP_001262614.1	alpha-Mannosidase class II b, isoform B
NM_001300412.1	NP_001287341.1	alpha-Mannosidase class II b, isoform C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004032	XP_309585.5
Drosophila melanogaster (fruit fly)	alpha-Man-IIb	NP_650494.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following alpha-Man-IIb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alpha-Man-IIb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05040	NM_142237.3 Latest version!	Drosophila melanogaster alpha-Mannosidase class II b (alpha-Man-IIb), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa05040	NM_001275685.1 Latest version!	Drosophila melanogaster alpha-Mannosidase class II b (alpha-Man-IIb), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa05040	NM_001300412.1 Latest version!	Drosophila melanogaster alpha-Mannosidase class II b (alpha-Man-IIb), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alpha-Man-IIb ( NM_142237.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650494.2 Drosophila melanogaster alpha-Mannosidase class II b (alpha-Man-IIb), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05040
Clone ID Related Accession (Same CDS sequence)	NM_142237.3 , NM_001275685.1 , NM_001300412.1
Accession Version	NM_142237.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha-Mannosidase class II b, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142237.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTCA AGCTATTCCG TCGCGGATCG GCGCGCTGCA TCGGTCTGCT ATCGGCGTTC 
GTAACGATCC TGCTCTGCCT CTACTACATC TCCATTGGCC AGCCCAATCC GACGCCCATT 
TCCGAGACGG GTGCCCCATC GGCGGCGTTA CGGGGATCAC CACTTAGTGG CCTAACTGGT 
TTCAGCGTCG ACGGAGGTTC GTCCTCGATC ATCCACCAAC AGAAGCAGCA ACTTGTGATC 
GCCTCAAACC AAAAGAAATC CTCGGAAACA AAGTCGGCGC AAACATCGGT ATCGCCGGAG 
GAGCAATCAG AGGAGCAGTA CACCAACAGC AATTGGGGCG ACAAGTGCTA CGAACTAATC 
CAGAGCAATA CAAACATAAC CGCTAGCGAG GAGCACAGCA AGTTTGACTT TCAGCCGGAA 
TGGATGCGAT CGAAGGAGTA CTGGGATCGT GGCTTTGAGG AGCGATTTGA TGCGCAGAAG 
AAGGACAAGC AGCGCCCGCC ACTAAAGATC ATAGTGGTGC CGCACTCCCA TAACGATCCT 
GGCTGGCTAA AGACCTTTAC CAACTACTTC CAATCGGATT CTCGGCAGAT TCTTAACCTA 
CTGGTGACCA AGATGCAGGA ATACACGGAC ATGACGTTCA TTTGGTCGGA GATCAGCTTC 
CTTCAGCTGT GGTGGGATCA AGCGCATCCA ACCAAGCAAC GGGCACTCAA GCGTTTAATC 
GACGCTGGTC GCATTGAAAT CACCACCGGT GGATGGGTGA TGACGGATGA GGCGAATGTC 
CACATCTATC CGATGTTAGA TCAGCTCATC GAGGGCCATC AGTGGCTAAA GAACAACCTG 
AATGTCACAC CGAAAGTGGG CTGGTCCATA GATCCATTTG GGCATGGCAG CACGGTGCCT 
TATCTGCTGT CCGGAGCGAA TTTCGAGGGA ACAATTATCC AGCGGATTCA TTACGCCTGG 
AAGCAGTGGT TTGCTCGCCA ACAGTCGGGT GACTTCATCT GGAAACCCTA TTGGAGAAGT 
AGAAAAGACA AGGATAGTGA AGCTGGTAGC CTTCTCACTC ACAACATGCC CTTCGATATA 
TACTCGATTA AGGGATCGTG TGGCCCACAT CCCTTCATAT GTCTGAACTT TGACTTTCGC 
AAAATTCCAG GCGAGTACAC CGAGTACTCC GTTAAGGCGC AGTTCATCAC AGACGACAAT 
CTGGAGTCAA AGGCGCAGTT GCTACTGGAG CAATATGCCA GGACTGCCTC GTTGTTTCCA 
CATAATGTGG CTCTCATTCC AGTCGGTGAT GATTTCCGAT ACAACAAGGA TCGCGAGGTG 
GATCAGCAGT ATCAGAACTA CAAGAAGCTT ATTGATCACA TCATGGCCAA TCGAAGGCTT 
TACAATGCTG ATATCCGATT TGGCACTCCC AGTGATTATT TCGAGGCCAT CAAGGAGCGC 
ATGAAGGATC ATACCCCAGG ATTTCCCACC TTTAAGGGTG ACTTCTTTGT TTATTCCGAT 
ATCTTTTCGG AGGGCAGACC GGCCTATTGG TCCGGTTACT TCACCACGCG TCCGTTCTAC 
AAGCTTCTGA GTTCAGAGCT GGAACATCAG CTGCGATCTG CTGAAATTGT CTTCACCCTG 
GCCTACAACA CAGCTCGACA AGCAAAGCGA GAGAATGCGA TAAAGATATA CGAGAAGAAC 
TACGAGCTTA TTATTAATGC AAGGCGAAAT TTGGGGCTGT TTCAGCACCA CGATGCCATC 
ACTGGTACAT CCAAGGCAGC GGTAATGAGG GACTATGCAA TGCGCCTGTT CGAGAGCACG 
CAAAACATGG TCAAGATGCA GGAATCCTGC CTAGAGCTCC TTCTACAAAA GGGCCAGCCT 
AGACATCACG GTTTCCTGCT CAGTGAATTT GAGCGAGATA ACTTCAACAA GCTGCCGCGC 
AAGATGCCGA TTCAGATGGC CAACGGGGAC GAGTCCACGC CCACTGTTCC GGCGGGAGAA 
GGCGGAGCCG GCGGATCATC TTCCAGCGGT TCGGTAGGCA GCTTTGTCCT GTACAATTCG 
CTAGCTCAGA AGCGGTTGGA AGTCGTCACG CTACGCGTCC AACATCCCAA TGTAAAGATA 
GTCAACGATA AGGGTGTAGA ATTGAATCAC ATCCAGATCA ATCCAGTGTG GAACATCACG 
GATACCTACG AACAGGGTCT GGGCACATCC GTATCCGGCA CTGTGGGTCG CATTCGCACT 
TCGACGCGCC AATTCGAGGT GATGTTCGTG GCGGAGCTGG AACCTTTATC GCTGAGCACT 
TATCGGGTAC AGGTGGACGA GGTCAACTTC AAGCGAAACA TAGCAACTAT ATACTGTGAC 
GATTGCACAG AATCGCAGCC AGCATCAGTG AGTCCGCCCG AAGAAATAGA ATCCTCACCG 
ACGTCAGTAT TTGAGGCGCG TGGCAAGCCA GCGGGCGATA TACAGTTGGA GAATCCTCAC 
ATGCGATTGC TGTTCGACGA GAAGAGCGGT TTTCTGAAGA CCATCACGCG TAAGAACCAG 
AAGAAGGAGT TGCTCAAGCC GCTTCAGTGC AACATCAAGT TTGCCGCTTA TCGCAGCGCC 
CAATTCCACT CTGGTGCATA TCTCTTCAAG ACCGATCCCG AACAAAGTGA GGCCGAGAAA 
GAAGTGCTTG AGGGCTATAC GGACATGCGC ATCATTATTA CCTCGGGTCC CATAGCCAGT 
GATGTGACTG TCATTTATGG ACCATTTTTG GCGCACACAG TGCGCATCTT CAACACGAGA 
ACTCATCTGG ATGCAGCGAT ATATGTTGAA AATGATATAG ATTTCGAGCC TCCACCCAAG 
AATCGGGAGA CGGAGCTGTT TATGCGATTT GTCACCAACA TAGACAATAT AGCTCAAGCT 
CCGATGCAGC GGGATCCGCT TAAGGAGCCG GTCGATGCGG CTACCATGCC GGAGCTTCCC 
GTCTTCTACA GCGATCAAAA CGGTTTCCAG TATCATGAAC GTATCAAAGT GCCGGCTATT 
GGCATCGAGG GCAACTACTT CCCGATCACC TCGGGAGCTT TTATTCAGGA CAGTCGCCTT 
CGCCTCACCC TGTTGACCAC TCATGCCCAA GGAGCAGCCA GTTATGAGCC TGGACAGCTG 
GAGGTTATGC TGGATCGGCG CACCCTGTAC GACGATTATC GGGGAATGGG CGAAGGCGTC 
GTGGACAGCC GTTTGACGCG GCACAAGTTC TGGCTGCTCG TGGAGGACCT CCCGGGTGGT 
CAGCATGTTA CGCAGCCACC GAGTTACAAG GTTCTCAGTC AGCAGGCTCA GCAGCTGGCA 
AATGCCTTGC GTTACCCGCC GAATCTGTAC TTTGTAAGCA ACCTGGAGCA GCCAGAACTG 
GCCGCTGCAT TGCTGCCTTT GGTCAGGCTT TTGCCTAAGG GACCGCTGCC CTGTGACGTA 
CACCTAACCA CCCTGCGTAC CCTATCCGAT CCGGAGTTGC AACTGTTTCC GTCCGCCTCG 
GCGCTGCTAG TTTTGCATCG CCAAGGATTC GATTGCAGTG TAAGCGCTGG CAGGGAATTG 
TCTATGGAGG CCATATGTCC CCTGTCCAGC AAGGGATTGG GTAATGTGCA GCTAGGTCGG 
CTCAGCCTGC ATACAATCGA AGCCACCAGC TTGACGGGGA CGGAGCAGAA ATCGTCCAGG 
GAGAGCTACC ACATACGCTC TCTGGCCGAG ATCAGTCTGG AACCAATGGA GCTACGCACC 
TTTAACCTTA CTTTCCGCTC GGAGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650494.2
CDS	454..4203
Translation	MSFKLFRRGSARCIGLLSAFVTILLCLYYISIGQPNPTPISETGAPSAALRGSPLSGLTGFSVDGGSSSIIHQQKQQLVIASNQKKSSETKSAQTSVSPEEQSEEQYTNSNWGDKCYELIQSNTNITASEEHSKFDFQPEWMRSKEYWDRGFEERFDAQKKDKQRPPLKIIVVPHSHNDPGWLKTFTNYFQSDSRQILNLLVTKMQEYTDMTFIWSEISFLQLWWDQAHPTKQRALKRLIDAGRIEITTGGWVMTDEANVHIYPMLDQLIEGHQWLKNNLNVTPKVGWSIDPFGHGSTVPYLLSGANFEGTIIQRIHYAWKQWFARQQSGDFIWKPYWRSRKDKDSEAGSLLTHNMPFDIYSIKGSCGPHPFICLNFDFRKIPGEYTEYSVKAQFITDDNLESKAQLLLEQYARTASLFPHNVALIPVGDDFRYNKDREVDQQYQNYKKLIDHIMANRRLYNADIRFGTPSDYFEAIKERMKDHTPGFPTFKGDFFVYSDIFSEGRPAYWSGYFTTRPFYKLLSSELEHQLRSAEIVFTLAYNTARQAKRENAIKIYEKNYELIINARRNLGLFQHHDAITGTSKAAVMRDYAMRLFESTQNMVKMQESCLELLLQKGQPRHHGFLLSEFERDNFNKLPRKMPIQMANGDESTPTVPAGEGGAGGSSSSGSVGSFVLYNSLAQKRLEVVTLRVQHPNVKIVNDKGVELNHIQINPVWNITDTYEQGLGTSVSGTVGRIRTSTRQFEVMFVAELEPLSLSTYRVQVDEVNFKRNIATIYCDDCTESQPASVSPPEEIESSPTSVFEARGKPAGDIQLENPHMRLLFDEKSGFLKTITRKNQKKELLKPLQCNIKFAAYRSAQFHSGAYLFKTDPEQSEAEKEVLEGYTDMRIIITSGPIASDVTVIYGPFLAHTVRIFNTRTHLDAAIYVENDIDFEPPPKNRETELFMRFVTNIDNIAQAPMQRDPLKEPVDAATMPELPVFYSDQNGFQYHERIKVPAIGIEGNYFPITSGAFIQDSRLRLTLLTTHAQGAASYEPGQLEVMLDRRTLYDDYRGMGEGVVDSRLTRHKFWLLVEDLPGGQHVTQPPSYKVLSQQAQQLANALRYPPNLYFVSNLEQPELAAALLPLVRLLPKGPLPCDVHLTTLRTLSDPELQLFPSASALLVLHRQGFDCSVSAGRELSMEAICPLSSKGLGNVQLGRLSLHTIEATSLTGTEQKSSRESYHIRSLAEISLEPMELRTFNLTFRSEV

Target ORF information:

RefSeq Version	NM_142237.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha-Mannosidase class II b (alpha-Man-IIb), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142237.3

ORF Insert Sequence:

ATGTCGTTCA AGCTATTCCG TCGCGGATCG GCGCGCTGCA TCGGTCTGCT ATCGGCGTTC 
GTAACGATCC TGCTCTGCCT CTACTACATC TCCATTGGCC AGCCCAATCC GACGCCCATT 
TCCGAGACGG GTGCCCCATC GGCGGCGTTA CGGGGATCAC CACTTAGTGG CCTAACTGGT 
TTCAGCGTCG ACGGAGGTTC GTCCTCGATC ATCCACCAAC AGAAGCAGCA ACTTGTGATC 
GCCTCAAACC AAAAGAAATC CTCGGAAACA AAGTCGGCGC AAACATCGGT ATCGCCGGAG 
GAGCAATCAG AGGAGCAGTA CACCAACAGC AATTGGGGCG ACAAGTGCTA CGAACTAATC 
CAGAGCAATA CAAACATAAC CGCTAGCGAG GAGCACAGCA AGTTTGACTT TCAGCCGGAA 
TGGATGCGAT CGAAGGAGTA CTGGGATCGT GGCTTTGAGG AGCGATTTGA TGCGCAGAAG 
AAGGACAAGC AGCGCCCGCC ACTAAAGATC ATAGTGGTGC CGCACTCCCA TAACGATCCT 
GGCTGGCTAA AGACCTTTAC CAACTACTTC CAATCGGATT CTCGGCAGAT TCTTAACCTA 
CTGGTGACCA AGATGCAGGA ATACACGGAC ATGACGTTCA TTTGGTCGGA GATCAGCTTC 
CTTCAGCTGT GGTGGGATCA AGCGCATCCA ACCAAGCAAC GGGCACTCAA GCGTTTAATC 
GACGCTGGTC GCATTGAAAT CACCACCGGT GGATGGGTGA TGACGGATGA GGCGAATGTC 
CACATCTATC CGATGTTAGA TCAGCTCATC GAGGGCCATC AGTGGCTAAA GAACAACCTG 
AATGTCACAC CGAAAGTGGG CTGGTCCATA GATCCATTTG GGCATGGCAG CACGGTGCCT 
TATCTGCTGT CCGGAGCGAA TTTCGAGGGA ACAATTATCC AGCGGATTCA TTACGCCTGG 
AAGCAGTGGT TTGCTCGCCA ACAGTCGGGT GACTTCATCT GGAAACCCTA TTGGAGAAGT 
AGAAAAGACA AGGATAGTGA AGCTGGTAGC CTTCTCACTC ACAACATGCC CTTCGATATA 
TACTCGATTA AGGGATCGTG TGGCCCACAT CCCTTCATAT GTCTGAACTT TGACTTTCGC 
AAAATTCCAG GCGAGTACAC CGAGTACTCC GTTAAGGCGC AGTTCATCAC AGACGACAAT 
CTGGAGTCAA AGGCGCAGTT GCTACTGGAG CAATATGCCA GGACTGCCTC GTTGTTTCCA 
CATAATGTGG CTCTCATTCC AGTCGGTGAT GATTTCCGAT ACAACAAGGA TCGCGAGGTG 
GATCAGCAGT ATCAGAACTA CAAGAAGCTT ATTGATCACA TCATGGCCAA TCGAAGGCTT 
TACAATGCTG ATATCCGATT TGGCACTCCC AGTGATTATT TCGAGGCCAT CAAGGAGCGC 
ATGAAGGATC ATACCCCAGG ATTTCCCACC TTTAAGGGTG ACTTCTTTGT TTATTCCGAT 
ATCTTTTCGG AGGGCAGACC GGCCTATTGG TCCGGTTACT TCACCACGCG TCCGTTCTAC 
AAGCTTCTGA GTTCAGAGCT GGAACATCAG CTGCGATCTG CTGAAATTGT CTTCACCCTG 
GCCTACAACA CAGCTCGACA AGCAAAGCGA GAGAATGCGA TAAAGATATA CGAGAAGAAC 
TACGAGCTTA TTATTAATGC AAGGCGAAAT TTGGGGCTGT TTCAGCACCA CGATGCCATC 
ACTGGTACAT CCAAGGCAGC GGTAATGAGG GACTATGCAA TGCGCCTGTT CGAGAGCACG 
CAAAACATGG TCAAGATGCA GGAATCCTGC CTAGAGCTCC TTCTACAAAA GGGCCAGCCT 
AGACATCACG GTTTCCTGCT CAGTGAATTT GAGCGAGATA ACTTCAACAA GCTGCCGCGC 
AAGATGCCGA TTCAGATGGC CAACGGGGAC GAGTCCACGC CCACTGTTCC GGCGGGAGAA 
GGCGGAGCCG GCGGATCATC TTCCAGCGGT TCGGTAGGCA GCTTTGTCCT GTACAATTCG 
CTAGCTCAGA AGCGGTTGGA AGTCGTCACG CTACGCGTCC AACATCCCAA TGTAAAGATA 
GTCAACGATA AGGGTGTAGA ATTGAATCAC ATCCAGATCA ATCCAGTGTG GAACATCACG 
GATACCTACG AACAGGGTCT GGGCACATCC GTATCCGGCA CTGTGGGTCG CATTCGCACT 
TCGACGCGCC AATTCGAGGT GATGTTCGTG GCGGAGCTGG AACCTTTATC GCTGAGCACT 
TATCGGGTAC AGGTGGACGA GGTCAACTTC AAGCGAAACA TAGCAACTAT ATACTGTGAC 
GATTGCACAG AATCGCAGCC AGCATCAGTG AGTCCGCCCG AAGAAATAGA ATCCTCACCG 
ACGTCAGTAT TTGAGGCGCG TGGCAAGCCA GCGGGCGATA TACAGTTGGA GAATCCTCAC 
ATGCGATTGC TGTTCGACGA GAAGAGCGGT TTTCTGAAGA CCATCACGCG TAAGAACCAG 
AAGAAGGAGT TGCTCAAGCC GCTTCAGTGC AACATCAAGT TTGCCGCTTA TCGCAGCGCC 
CAATTCCACT CTGGTGCATA TCTCTTCAAG ACCGATCCCG AACAAAGTGA GGCCGAGAAA 
GAAGTGCTTG AGGGCTATAC GGACATGCGC ATCATTATTA CCTCGGGTCC CATAGCCAGT 
GATGTGACTG TCATTTATGG ACCATTTTTG GCGCACACAG TGCGCATCTT CAACACGAGA 
ACTCATCTGG ATGCAGCGAT ATATGTTGAA AATGATATAG ATTTCGAGCC TCCACCCAAG 
AATCGGGAGA CGGAGCTGTT TATGCGATTT GTCACCAACA TAGACAATAT AGCTCAAGCT 
CCGATGCAGC GGGATCCGCT TAAGGAGCCG GTCGATGCGG CTACCATGCC GGAGCTTCCC 
GTCTTCTACA GCGATCAAAA CGGTTTCCAG TATCATGAAC GTATCAAAGT GCCGGCTATT 
GGCATCGAGG GCAACTACTT CCCGATCACC TCGGGAGCTT TTATTCAGGA CAGTCGCCTT 
CGCCTCACCC TGTTGACCAC TCATGCCCAA GGAGCAGCCA GTTATGAGCC TGGACAGCTG 
GAGGTTATGC TGGATCGGCG CACCCTGTAC GACGATTATC GGGGAATGGG CGAAGGCGTC 
GTGGACAGCC GTTTGACGCG GCACAAGTTC TGGCTGCTCG TGGAGGACCT CCCGGGTGGT 
CAGCATGTTA CGCAGCCACC GAGTTACAAG GTTCTCAGTC AGCAGGCTCA GCAGCTGGCA 
AATGCCTTGC GTTACCCGCC GAATCTGTAC TTTGTAAGCA ACCTGGAGCA GCCAGAACTG 
GCCGCTGCAT TGCTGCCTTT GGTCAGGCTT TTGCCTAAGG GACCGCTGCC CTGTGACGTA 
CACCTAACCA CCCTGCGTAC CCTATCCGAT CCGGAGTTGC AACTGTTTCC GTCCGCCTCG 
GCGCTGCTAG TTTTGCATCG CCAAGGATTC GATTGCAGTG TAAGCGCTGG CAGGGAATTG 
TCTATGGAGG CCATATGTCC CCTGTCCAGC AAGGGATTGG GTAATGTGCA GCTAGGTCGG 
CTCAGCCTGC ATACAATCGA AGCCACCAGC TTGACGGGGA CGGAGCAGAA ATCGTCCAGG 
GAGAGCTACC ACATACGCTC TCTGGCCGAG ATCAGTCTGG AACCAATGGA GCTACGCACC 
TTTAACCTTA CTTTCCGCTC GGAGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alpha-Man-IIb ( NM_001275685.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262614.1 Drosophila melanogaster alpha-Mannosidase class II b (alpha-Man-IIb), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05040
Clone ID Related Accession (Same CDS sequence)	NM_142237.3 , NM_001275685.1 , NM_001300412.1
Accession Version	NM_001275685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha-Mannosidase class II b, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTCA AGCTATTCCG TCGCGGATCG GCGCGCTGCA TCGGTCTGCT ATCGGCGTTC 
GTAACGATCC TGCTCTGCCT CTACTACATC TCCATTGGCC AGCCCAATCC GACGCCCATT 
TCCGAGACGG GTGCCCCATC GGCGGCGTTA CGGGGATCAC CACTTAGTGG CCTAACTGGT 
TTCAGCGTCG ACGGAGGTTC GTCCTCGATC ATCCACCAAC AGAAGCAGCA ACTTGTGATC 
GCCTCAAACC AAAAGAAATC CTCGGAAACA AAGTCGGCGC AAACATCGGT ATCGCCGGAG 
GAGCAATCAG AGGAGCAGTA CACCAACAGC AATTGGGGCG ACAAGTGCTA CGAACTAATC 
CAGAGCAATA CAAACATAAC CGCTAGCGAG GAGCACAGCA AGTTTGACTT TCAGCCGGAA 
TGGATGCGAT CGAAGGAGTA CTGGGATCGT GGCTTTGAGG AGCGATTTGA TGCGCAGAAG 
AAGGACAAGC AGCGCCCGCC ACTAAAGATC ATAGTGGTGC CGCACTCCCA TAACGATCCT 
GGCTGGCTAA AGACCTTTAC CAACTACTTC CAATCGGATT CTCGGCAGAT TCTTAACCTA 
CTGGTGACCA AGATGCAGGA ATACACGGAC ATGACGTTCA TTTGGTCGGA GATCAGCTTC 
CTTCAGCTGT GGTGGGATCA AGCGCATCCA ACCAAGCAAC GGGCACTCAA GCGTTTAATC 
GACGCTGGTC GCATTGAAAT CACCACCGGT GGATGGGTGA TGACGGATGA GGCGAATGTC 
CACATCTATC CGATGTTAGA TCAGCTCATC GAGGGCCATC AGTGGCTAAA GAACAACCTG 
AATGTCACAC CGAAAGTGGG CTGGTCCATA GATCCATTTG GGCATGGCAG CACGGTGCCT 
TATCTGCTGT CCGGAGCGAA TTTCGAGGGA ACAATTATCC AGCGGATTCA TTACGCCTGG 
AAGCAGTGGT TTGCTCGCCA ACAGTCGGGT GACTTCATCT GGAAACCCTA TTGGAGAAGT 
AGAAAAGACA AGGATAGTGA AGCTGGTAGC CTTCTCACTC ACAACATGCC CTTCGATATA 
TACTCGATTA AGGGATCGTG TGGCCCACAT CCCTTCATAT GTCTGAACTT TGACTTTCGC 
AAAATTCCAG GCGAGTACAC CGAGTACTCC GTTAAGGCGC AGTTCATCAC AGACGACAAT 
CTGGAGTCAA AGGCGCAGTT GCTACTGGAG CAATATGCCA GGACTGCCTC GTTGTTTCCA 
CATAATGTGG CTCTCATTCC AGTCGGTGAT GATTTCCGAT ACAACAAGGA TCGCGAGGTG 
GATCAGCAGT ATCAGAACTA CAAGAAGCTT ATTGATCACA TCATGGCCAA TCGAAGGCTT 
TACAATGCTG ATATCCGATT TGGCACTCCC AGTGATTATT TCGAGGCCAT CAAGGAGCGC 
ATGAAGGATC ATACCCCAGG ATTTCCCACC TTTAAGGGTG ACTTCTTTGT TTATTCCGAT 
ATCTTTTCGG AGGGCAGACC GGCCTATTGG TCCGGTTACT TCACCACGCG TCCGTTCTAC 
AAGCTTCTGA GTTCAGAGCT GGAACATCAG CTGCGATCTG CTGAAATTGT CTTCACCCTG 
GCCTACAACA CAGCTCGACA AGCAAAGCGA GAGAATGCGA TAAAGATATA CGAGAAGAAC 
TACGAGCTTA TTATTAATGC AAGGCGAAAT TTGGGGCTGT TTCAGCACCA CGATGCCATC 
ACTGGTACAT CCAAGGCAGC GGTAATGAGG GACTATGCAA TGCGCCTGTT CGAGAGCACG 
CAAAACATGG TCAAGATGCA GGAATCCTGC CTAGAGCTCC TTCTACAAAA GGGCCAGCCT 
AGACATCACG GTTTCCTGCT CAGTGAATTT GAGCGAGATA ACTTCAACAA GCTGCCGCGC 
AAGATGCCGA TTCAGATGGC CAACGGGGAC GAGTCCACGC CCACTGTTCC GGCGGGAGAA 
GGCGGAGCCG GCGGATCATC TTCCAGCGGT TCGGTAGGCA GCTTTGTCCT GTACAATTCG 
CTAGCTCAGA AGCGGTTGGA AGTCGTCACG CTACGCGTCC AACATCCCAA TGTAAAGATA 
GTCAACGATA AGGGTGTAGA ATTGAATCAC ATCCAGATCA ATCCAGTGTG GAACATCACG 
GATACCTACG AACAGGGTCT GGGCACATCC GTATCCGGCA CTGTGGGTCG CATTCGCACT 
TCGACGCGCC AATTCGAGGT GATGTTCGTG GCGGAGCTGG AACCTTTATC GCTGAGCACT 
TATCGGGTAC AGGTGGACGA GGTCAACTTC AAGCGAAACA TAGCAACTAT ATACTGTGAC 
GATTGCACAG AATCGCAGCC AGCATCAGTG AGTCCGCCCG AAGAAATAGA ATCCTCACCG 
ACGTCAGTAT TTGAGGCGCG TGGCAAGCCA GCGGGCGATA TACAGTTGGA GAATCCTCAC 
ATGCGATTGC TGTTCGACGA GAAGAGCGGT TTTCTGAAGA CCATCACGCG TAAGAACCAG 
AAGAAGGAGT TGCTCAAGCC GCTTCAGTGC AACATCAAGT TTGCCGCTTA TCGCAGCGCC 
CAATTCCACT CTGGTGCATA TCTCTTCAAG ACCGATCCCG AACAAAGTGA GGCCGAGAAA 
GAAGTGCTTG AGGGCTATAC GGACATGCGC ATCATTATTA CCTCGGGTCC CATAGCCAGT 
GATGTGACTG TCATTTATGG ACCATTTTTG GCGCACACAG TGCGCATCTT CAACACGAGA 
ACTCATCTGG ATGCAGCGAT ATATGTTGAA AATGATATAG ATTTCGAGCC TCCACCCAAG 
AATCGGGAGA CGGAGCTGTT TATGCGATTT GTCACCAACA TAGACAATAT AGCTCAAGCT 
CCGATGCAGC GGGATCCGCT TAAGGAGCCG GTCGATGCGG CTACCATGCC GGAGCTTCCC 
GTCTTCTACA GCGATCAAAA CGGTTTCCAG TATCATGAAC GTATCAAAGT GCCGGCTATT 
GGCATCGAGG GCAACTACTT CCCGATCACC TCGGGAGCTT TTATTCAGGA CAGTCGCCTT 
CGCCTCACCC TGTTGACCAC TCATGCCCAA GGAGCAGCCA GTTATGAGCC TGGACAGCTG 
GAGGTTATGC TGGATCGGCG CACCCTGTAC GACGATTATC GGGGAATGGG CGAAGGCGTC 
GTGGACAGCC GTTTGACGCG GCACAAGTTC TGGCTGCTCG TGGAGGACCT CCCGGGTGGT 
CAGCATGTTA CGCAGCCACC GAGTTACAAG GTTCTCAGTC AGCAGGCTCA GCAGCTGGCA 
AATGCCTTGC GTTACCCGCC GAATCTGTAC TTTGTAAGCA ACCTGGAGCA GCCAGAACTG 
GCCGCTGCAT TGCTGCCTTT GGTCAGGCTT TTGCCTAAGG GACCGCTGCC CTGTGACGTA 
CACCTAACCA CCCTGCGTAC CCTATCCGAT CCGGAGTTGC AACTGTTTCC GTCCGCCTCG 
GCGCTGCTAG TTTTGCATCG CCAAGGATTC GATTGCAGTG TAAGCGCTGG CAGGGAATTG 
TCTATGGAGG CCATATGTCC CCTGTCCAGC AAGGGATTGG GTAATGTGCA GCTAGGTCGG 
CTCAGCCTGC ATACAATCGA AGCCACCAGC TTGACGGGGA CGGAGCAGAA ATCGTCCAGG 
GAGAGCTACC ACATACGCTC TCTGGCCGAG ATCAGTCTGG AACCAATGGA GCTACGCACC 
TTTAACCTTA CTTTCCGCTC GGAGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262614.1
CDS	556..4305
Translation	MSFKLFRRGSARCIGLLSAFVTILLCLYYISIGQPNPTPISETGAPSAALRGSPLSGLTGFSVDGGSSSIIHQQKQQLVIASNQKKSSETKSAQTSVSPEEQSEEQYTNSNWGDKCYELIQSNTNITASEEHSKFDFQPEWMRSKEYWDRGFEERFDAQKKDKQRPPLKIIVVPHSHNDPGWLKTFTNYFQSDSRQILNLLVTKMQEYTDMTFIWSEISFLQLWWDQAHPTKQRALKRLIDAGRIEITTGGWVMTDEANVHIYPMLDQLIEGHQWLKNNLNVTPKVGWSIDPFGHGSTVPYLLSGANFEGTIIQRIHYAWKQWFARQQSGDFIWKPYWRSRKDKDSEAGSLLTHNMPFDIYSIKGSCGPHPFICLNFDFRKIPGEYTEYSVKAQFITDDNLESKAQLLLEQYARTASLFPHNVALIPVGDDFRYNKDREVDQQYQNYKKLIDHIMANRRLYNADIRFGTPSDYFEAIKERMKDHTPGFPTFKGDFFVYSDIFSEGRPAYWSGYFTTRPFYKLLSSELEHQLRSAEIVFTLAYNTARQAKRENAIKIYEKNYELIINARRNLGLFQHHDAITGTSKAAVMRDYAMRLFESTQNMVKMQESCLELLLQKGQPRHHGFLLSEFERDNFNKLPRKMPIQMANGDESTPTVPAGEGGAGGSSSSGSVGSFVLYNSLAQKRLEVVTLRVQHPNVKIVNDKGVELNHIQINPVWNITDTYEQGLGTSVSGTVGRIRTSTRQFEVMFVAELEPLSLSTYRVQVDEVNFKRNIATIYCDDCTESQPASVSPPEEIESSPTSVFEARGKPAGDIQLENPHMRLLFDEKSGFLKTITRKNQKKELLKPLQCNIKFAAYRSAQFHSGAYLFKTDPEQSEAEKEVLEGYTDMRIIITSGPIASDVTVIYGPFLAHTVRIFNTRTHLDAAIYVENDIDFEPPPKNRETELFMRFVTNIDNIAQAPMQRDPLKEPVDAATMPELPVFYSDQNGFQYHERIKVPAIGIEGNYFPITSGAFIQDSRLRLTLLTTHAQGAASYEPGQLEVMLDRRTLYDDYRGMGEGVVDSRLTRHKFWLLVEDLPGGQHVTQPPSYKVLSQQAQQLANALRYPPNLYFVSNLEQPELAAALLPLVRLLPKGPLPCDVHLTTLRTLSDPELQLFPSASALLVLHRQGFDCSVSAGRELSMEAICPLSSKGLGNVQLGRLSLHTIEATSLTGTEQKSSRESYHIRSLAEISLEPMELRTFNLTFRSEV

Target ORF information:

RefSeq Version	NM_001275685.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha-Mannosidase class II b (alpha-Man-IIb), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275685.1

ORF Insert Sequence:

ATGTCGTTCA AGCTATTCCG TCGCGGATCG GCGCGCTGCA TCGGTCTGCT ATCGGCGTTC 
GTAACGATCC TGCTCTGCCT CTACTACATC TCCATTGGCC AGCCCAATCC GACGCCCATT 
TCCGAGACGG GTGCCCCATC GGCGGCGTTA CGGGGATCAC CACTTAGTGG CCTAACTGGT 
TTCAGCGTCG ACGGAGGTTC GTCCTCGATC ATCCACCAAC AGAAGCAGCA ACTTGTGATC 
GCCTCAAACC AAAAGAAATC CTCGGAAACA AAGTCGGCGC AAACATCGGT ATCGCCGGAG 
GAGCAATCAG AGGAGCAGTA CACCAACAGC AATTGGGGCG ACAAGTGCTA CGAACTAATC 
CAGAGCAATA CAAACATAAC CGCTAGCGAG GAGCACAGCA AGTTTGACTT TCAGCCGGAA 
TGGATGCGAT CGAAGGAGTA CTGGGATCGT GGCTTTGAGG AGCGATTTGA TGCGCAGAAG 
AAGGACAAGC AGCGCCCGCC ACTAAAGATC ATAGTGGTGC CGCACTCCCA TAACGATCCT 
GGCTGGCTAA AGACCTTTAC CAACTACTTC CAATCGGATT CTCGGCAGAT TCTTAACCTA 
CTGGTGACCA AGATGCAGGA ATACACGGAC ATGACGTTCA TTTGGTCGGA GATCAGCTTC 
CTTCAGCTGT GGTGGGATCA AGCGCATCCA ACCAAGCAAC GGGCACTCAA GCGTTTAATC 
GACGCTGGTC GCATTGAAAT CACCACCGGT GGATGGGTGA TGACGGATGA GGCGAATGTC 
CACATCTATC CGATGTTAGA TCAGCTCATC GAGGGCCATC AGTGGCTAAA GAACAACCTG 
AATGTCACAC CGAAAGTGGG CTGGTCCATA GATCCATTTG GGCATGGCAG CACGGTGCCT 
TATCTGCTGT CCGGAGCGAA TTTCGAGGGA ACAATTATCC AGCGGATTCA TTACGCCTGG 
AAGCAGTGGT TTGCTCGCCA ACAGTCGGGT GACTTCATCT GGAAACCCTA TTGGAGAAGT 
AGAAAAGACA AGGATAGTGA AGCTGGTAGC CTTCTCACTC ACAACATGCC CTTCGATATA 
TACTCGATTA AGGGATCGTG TGGCCCACAT CCCTTCATAT GTCTGAACTT TGACTTTCGC 
AAAATTCCAG GCGAGTACAC CGAGTACTCC GTTAAGGCGC AGTTCATCAC AGACGACAAT 
CTGGAGTCAA AGGCGCAGTT GCTACTGGAG CAATATGCCA GGACTGCCTC GTTGTTTCCA 
CATAATGTGG CTCTCATTCC AGTCGGTGAT GATTTCCGAT ACAACAAGGA TCGCGAGGTG 
GATCAGCAGT ATCAGAACTA CAAGAAGCTT ATTGATCACA TCATGGCCAA TCGAAGGCTT 
TACAATGCTG ATATCCGATT TGGCACTCCC AGTGATTATT TCGAGGCCAT CAAGGAGCGC 
ATGAAGGATC ATACCCCAGG ATTTCCCACC TTTAAGGGTG ACTTCTTTGT TTATTCCGAT 
ATCTTTTCGG AGGGCAGACC GGCCTATTGG TCCGGTTACT TCACCACGCG TCCGTTCTAC 
AAGCTTCTGA GTTCAGAGCT GGAACATCAG CTGCGATCTG CTGAAATTGT CTTCACCCTG 
GCCTACAACA CAGCTCGACA AGCAAAGCGA GAGAATGCGA TAAAGATATA CGAGAAGAAC 
TACGAGCTTA TTATTAATGC AAGGCGAAAT TTGGGGCTGT TTCAGCACCA CGATGCCATC 
ACTGGTACAT CCAAGGCAGC GGTAATGAGG GACTATGCAA TGCGCCTGTT CGAGAGCACG 
CAAAACATGG TCAAGATGCA GGAATCCTGC CTAGAGCTCC TTCTACAAAA GGGCCAGCCT 
AGACATCACG GTTTCCTGCT CAGTGAATTT GAGCGAGATA ACTTCAACAA GCTGCCGCGC 
AAGATGCCGA TTCAGATGGC CAACGGGGAC GAGTCCACGC CCACTGTTCC GGCGGGAGAA 
GGCGGAGCCG GCGGATCATC TTCCAGCGGT TCGGTAGGCA GCTTTGTCCT GTACAATTCG 
CTAGCTCAGA AGCGGTTGGA AGTCGTCACG CTACGCGTCC AACATCCCAA TGTAAAGATA 
GTCAACGATA AGGGTGTAGA ATTGAATCAC ATCCAGATCA ATCCAGTGTG GAACATCACG 
GATACCTACG AACAGGGTCT GGGCACATCC GTATCCGGCA CTGTGGGTCG CATTCGCACT 
TCGACGCGCC AATTCGAGGT GATGTTCGTG GCGGAGCTGG AACCTTTATC GCTGAGCACT 
TATCGGGTAC AGGTGGACGA GGTCAACTTC AAGCGAAACA TAGCAACTAT ATACTGTGAC 
GATTGCACAG AATCGCAGCC AGCATCAGTG AGTCCGCCCG AAGAAATAGA ATCCTCACCG 
ACGTCAGTAT TTGAGGCGCG TGGCAAGCCA GCGGGCGATA TACAGTTGGA GAATCCTCAC 
ATGCGATTGC TGTTCGACGA GAAGAGCGGT TTTCTGAAGA CCATCACGCG TAAGAACCAG 
AAGAAGGAGT TGCTCAAGCC GCTTCAGTGC AACATCAAGT TTGCCGCTTA TCGCAGCGCC 
CAATTCCACT CTGGTGCATA TCTCTTCAAG ACCGATCCCG AACAAAGTGA GGCCGAGAAA 
GAAGTGCTTG AGGGCTATAC GGACATGCGC ATCATTATTA CCTCGGGTCC CATAGCCAGT 
GATGTGACTG TCATTTATGG ACCATTTTTG GCGCACACAG TGCGCATCTT CAACACGAGA 
ACTCATCTGG ATGCAGCGAT ATATGTTGAA AATGATATAG ATTTCGAGCC TCCACCCAAG 
AATCGGGAGA CGGAGCTGTT TATGCGATTT GTCACCAACA TAGACAATAT AGCTCAAGCT 
CCGATGCAGC GGGATCCGCT TAAGGAGCCG GTCGATGCGG CTACCATGCC GGAGCTTCCC 
GTCTTCTACA GCGATCAAAA CGGTTTCCAG TATCATGAAC GTATCAAAGT GCCGGCTATT 
GGCATCGAGG GCAACTACTT CCCGATCACC TCGGGAGCTT TTATTCAGGA CAGTCGCCTT 
CGCCTCACCC TGTTGACCAC TCATGCCCAA GGAGCAGCCA GTTATGAGCC TGGACAGCTG 
GAGGTTATGC TGGATCGGCG CACCCTGTAC GACGATTATC GGGGAATGGG CGAAGGCGTC 
GTGGACAGCC GTTTGACGCG GCACAAGTTC TGGCTGCTCG TGGAGGACCT CCCGGGTGGT 
CAGCATGTTA CGCAGCCACC GAGTTACAAG GTTCTCAGTC AGCAGGCTCA GCAGCTGGCA 
AATGCCTTGC GTTACCCGCC GAATCTGTAC TTTGTAAGCA ACCTGGAGCA GCCAGAACTG 
GCCGCTGCAT TGCTGCCTTT GGTCAGGCTT TTGCCTAAGG GACCGCTGCC CTGTGACGTA 
CACCTAACCA CCCTGCGTAC CCTATCCGAT CCGGAGTTGC AACTGTTTCC GTCCGCCTCG 
GCGCTGCTAG TTTTGCATCG CCAAGGATTC GATTGCAGTG TAAGCGCTGG CAGGGAATTG 
TCTATGGAGG CCATATGTCC CCTGTCCAGC AAGGGATTGG GTAATGTGCA GCTAGGTCGG 
CTCAGCCTGC ATACAATCGA AGCCACCAGC TTGACGGGGA CGGAGCAGAA ATCGTCCAGG 
GAGAGCTACC ACATACGCTC TCTGGCCGAG ATCAGTCTGG AACCAATGGA GCTACGCACC 
TTTAACCTTA CTTTCCGCTC GGAGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alpha-Man-IIb ( NM_001300412.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287341.1 Drosophila melanogaster alpha-Mannosidase class II b (alpha-Man-IIb), transcript variant C, mRNA.

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CloneID	OFa05040
Clone ID Related Accession (Same CDS sequence)	NM_142237.3 , NM_001275685.1 , NM_001300412.1
Accession Version	NM_001300412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha-Mannosidase class II b, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTCA AGCTATTCCG TCGCGGATCG GCGCGCTGCA TCGGTCTGCT ATCGGCGTTC 
GTAACGATCC TGCTCTGCCT CTACTACATC TCCATTGGCC AGCCCAATCC GACGCCCATT 
TCCGAGACGG GTGCCCCATC GGCGGCGTTA CGGGGATCAC CACTTAGTGG CCTAACTGGT 
TTCAGCGTCG ACGGAGGTTC GTCCTCGATC ATCCACCAAC AGAAGCAGCA ACTTGTGATC 
GCCTCAAACC AAAAGAAATC CTCGGAAACA AAGTCGGCGC AAACATCGGT ATCGCCGGAG 
GAGCAATCAG AGGAGCAGTA CACCAACAGC AATTGGGGCG ACAAGTGCTA CGAACTAATC 
CAGAGCAATA CAAACATAAC CGCTAGCGAG GAGCACAGCA AGTTTGACTT TCAGCCGGAA 
TGGATGCGAT CGAAGGAGTA CTGGGATCGT GGCTTTGAGG AGCGATTTGA TGCGCAGAAG 
AAGGACAAGC AGCGCCCGCC ACTAAAGATC ATAGTGGTGC CGCACTCCCA TAACGATCCT 
GGCTGGCTAA AGACCTTTAC CAACTACTTC CAATCGGATT CTCGGCAGAT TCTTAACCTA 
CTGGTGACCA AGATGCAGGA ATACACGGAC ATGACGTTCA TTTGGTCGGA GATCAGCTTC 
CTTCAGCTGT GGTGGGATCA AGCGCATCCA ACCAAGCAAC GGGCACTCAA GCGTTTAATC 
GACGCTGGTC GCATTGAAAT CACCACCGGT GGATGGGTGA TGACGGATGA GGCGAATGTC 
CACATCTATC CGATGTTAGA TCAGCTCATC GAGGGCCATC AGTGGCTAAA GAACAACCTG 
AATGTCACAC CGAAAGTGGG CTGGTCCATA GATCCATTTG GGCATGGCAG CACGGTGCCT 
TATCTGCTGT CCGGAGCGAA TTTCGAGGGA ACAATTATCC AGCGGATTCA TTACGCCTGG 
AAGCAGTGGT TTGCTCGCCA ACAGTCGGGT GACTTCATCT GGAAACCCTA TTGGAGAAGT 
AGAAAAGACA AGGATAGTGA AGCTGGTAGC CTTCTCACTC ACAACATGCC CTTCGATATA 
TACTCGATTA AGGGATCGTG TGGCCCACAT CCCTTCATAT GTCTGAACTT TGACTTTCGC 
AAAATTCCAG GCGAGTACAC CGAGTACTCC GTTAAGGCGC AGTTCATCAC AGACGACAAT 
CTGGAGTCAA AGGCGCAGTT GCTACTGGAG CAATATGCCA GGACTGCCTC GTTGTTTCCA 
CATAATGTGG CTCTCATTCC AGTCGGTGAT GATTTCCGAT ACAACAAGGA TCGCGAGGTG 
GATCAGCAGT ATCAGAACTA CAAGAAGCTT ATTGATCACA TCATGGCCAA TCGAAGGCTT 
TACAATGCTG ATATCCGATT TGGCACTCCC AGTGATTATT TCGAGGCCAT CAAGGAGCGC 
ATGAAGGATC ATACCCCAGG ATTTCCCACC TTTAAGGGTG ACTTCTTTGT TTATTCCGAT 
ATCTTTTCGG AGGGCAGACC GGCCTATTGG TCCGGTTACT TCACCACGCG TCCGTTCTAC 
AAGCTTCTGA GTTCAGAGCT GGAACATCAG CTGCGATCTG CTGAAATTGT CTTCACCCTG 
GCCTACAACA CAGCTCGACA AGCAAAGCGA GAGAATGCGA TAAAGATATA CGAGAAGAAC 
TACGAGCTTA TTATTAATGC AAGGCGAAAT TTGGGGCTGT TTCAGCACCA CGATGCCATC 
ACTGGTACAT CCAAGGCAGC GGTAATGAGG GACTATGCAA TGCGCCTGTT CGAGAGCACG 
CAAAACATGG TCAAGATGCA GGAATCCTGC CTAGAGCTCC TTCTACAAAA GGGCCAGCCT 
AGACATCACG GTTTCCTGCT CAGTGAATTT GAGCGAGATA ACTTCAACAA GCTGCCGCGC 
AAGATGCCGA TTCAGATGGC CAACGGGGAC GAGTCCACGC CCACTGTTCC GGCGGGAGAA 
GGCGGAGCCG GCGGATCATC TTCCAGCGGT TCGGTAGGCA GCTTTGTCCT GTACAATTCG 
CTAGCTCAGA AGCGGTTGGA AGTCGTCACG CTACGCGTCC AACATCCCAA TGTAAAGATA 
GTCAACGATA AGGGTGTAGA ATTGAATCAC ATCCAGATCA ATCCAGTGTG GAACATCACG 
GATACCTACG AACAGGGTCT GGGCACATCC GTATCCGGCA CTGTGGGTCG CATTCGCACT 
TCGACGCGCC AATTCGAGGT GATGTTCGTG GCGGAGCTGG AACCTTTATC GCTGAGCACT 
TATCGGGTAC AGGTGGACGA GGTCAACTTC AAGCGAAACA TAGCAACTAT ATACTGTGAC 
GATTGCACAG AATCGCAGCC AGCATCAGTG AGTCCGCCCG AAGAAATAGA ATCCTCACCG 
ACGTCAGTAT TTGAGGCGCG TGGCAAGCCA GCGGGCGATA TACAGTTGGA GAATCCTCAC 
ATGCGATTGC TGTTCGACGA GAAGAGCGGT TTTCTGAAGA CCATCACGCG TAAGAACCAG 
AAGAAGGAGT TGCTCAAGCC GCTTCAGTGC AACATCAAGT TTGCCGCTTA TCGCAGCGCC 
CAATTCCACT CTGGTGCATA TCTCTTCAAG ACCGATCCCG AACAAAGTGA GGCCGAGAAA 
GAAGTGCTTG AGGGCTATAC GGACATGCGC ATCATTATTA CCTCGGGTCC CATAGCCAGT 
GATGTGACTG TCATTTATGG ACCATTTTTG GCGCACACAG TGCGCATCTT CAACACGAGA 
ACTCATCTGG ATGCAGCGAT ATATGTTGAA AATGATATAG ATTTCGAGCC TCCACCCAAG 
AATCGGGAGA CGGAGCTGTT TATGCGATTT GTCACCAACA TAGACAATAT AGCTCAAGCT 
CCGATGCAGC GGGATCCGCT TAAGGAGCCG GTCGATGCGG CTACCATGCC GGAGCTTCCC 
GTCTTCTACA GCGATCAAAA CGGTTTCCAG TATCATGAAC GTATCAAAGT GCCGGCTATT 
GGCATCGAGG GCAACTACTT CCCGATCACC TCGGGAGCTT TTATTCAGGA CAGTCGCCTT 
CGCCTCACCC TGTTGACCAC TCATGCCCAA GGAGCAGCCA GTTATGAGCC TGGACAGCTG 
GAGGTTATGC TGGATCGGCG CACCCTGTAC GACGATTATC GGGGAATGGG CGAAGGCGTC 
GTGGACAGCC GTTTGACGCG GCACAAGTTC TGGCTGCTCG TGGAGGACCT CCCGGGTGGT 
CAGCATGTTA CGCAGCCACC GAGTTACAAG GTTCTCAGTC AGCAGGCTCA GCAGCTGGCA 
AATGCCTTGC GTTACCCGCC GAATCTGTAC TTTGTAAGCA ACCTGGAGCA GCCAGAACTG 
GCCGCTGCAT TGCTGCCTTT GGTCAGGCTT TTGCCTAAGG GACCGCTGCC CTGTGACGTA 
CACCTAACCA CCCTGCGTAC CCTATCCGAT CCGGAGTTGC AACTGTTTCC GTCCGCCTCG 
GCGCTGCTAG TTTTGCATCG CCAAGGATTC GATTGCAGTG TAAGCGCTGG CAGGGAATTG 
TCTATGGAGG CCATATGTCC CCTGTCCAGC AAGGGATTGG GTAATGTGCA GCTAGGTCGG 
CTCAGCCTGC ATACAATCGA AGCCACCAGC TTGACGGGGA CGGAGCAGAA ATCGTCCAGG 
GAGAGCTACC ACATACGCTC TCTGGCCGAG ATCAGTCTGG AACCAATGGA GCTACGCACC 
TTTAACCTTA CTTTCCGCTC GGAGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287341.1
CDS	281..4030
Translation	MSFKLFRRGSARCIGLLSAFVTILLCLYYISIGQPNPTPISETGAPSAALRGSPLSGLTGFSVDGGSSSIIHQQKQQLVIASNQKKSSETKSAQTSVSPEEQSEEQYTNSNWGDKCYELIQSNTNITASEEHSKFDFQPEWMRSKEYWDRGFEERFDAQKKDKQRPPLKIIVVPHSHNDPGWLKTFTNYFQSDSRQILNLLVTKMQEYTDMTFIWSEISFLQLWWDQAHPTKQRALKRLIDAGRIEITTGGWVMTDEANVHIYPMLDQLIEGHQWLKNNLNVTPKVGWSIDPFGHGSTVPYLLSGANFEGTIIQRIHYAWKQWFARQQSGDFIWKPYWRSRKDKDSEAGSLLTHNMPFDIYSIKGSCGPHPFICLNFDFRKIPGEYTEYSVKAQFITDDNLESKAQLLLEQYARTASLFPHNVALIPVGDDFRYNKDREVDQQYQNYKKLIDHIMANRRLYNADIRFGTPSDYFEAIKERMKDHTPGFPTFKGDFFVYSDIFSEGRPAYWSGYFTTRPFYKLLSSELEHQLRSAEIVFTLAYNTARQAKRENAIKIYEKNYELIINARRNLGLFQHHDAITGTSKAAVMRDYAMRLFESTQNMVKMQESCLELLLQKGQPRHHGFLLSEFERDNFNKLPRKMPIQMANGDESTPTVPAGEGGAGGSSSSGSVGSFVLYNSLAQKRLEVVTLRVQHPNVKIVNDKGVELNHIQINPVWNITDTYEQGLGTSVSGTVGRIRTSTRQFEVMFVAELEPLSLSTYRVQVDEVNFKRNIATIYCDDCTESQPASVSPPEEIESSPTSVFEARGKPAGDIQLENPHMRLLFDEKSGFLKTITRKNQKKELLKPLQCNIKFAAYRSAQFHSGAYLFKTDPEQSEAEKEVLEGYTDMRIIITSGPIASDVTVIYGPFLAHTVRIFNTRTHLDAAIYVENDIDFEPPPKNRETELFMRFVTNIDNIAQAPMQRDPLKEPVDAATMPELPVFYSDQNGFQYHERIKVPAIGIEGNYFPITSGAFIQDSRLRLTLLTTHAQGAASYEPGQLEVMLDRRTLYDDYRGMGEGVVDSRLTRHKFWLLVEDLPGGQHVTQPPSYKVLSQQAQQLANALRYPPNLYFVSNLEQPELAAALLPLVRLLPKGPLPCDVHLTTLRTLSDPELQLFPSASALLVLHRQGFDCSVSAGRELSMEAICPLSSKGLGNVQLGRLSLHTIEATSLTGTEQKSSRESYHIRSLAEISLEPMELRTFNLTFRSEV

Target ORF information:

RefSeq Version	NM_001300412.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha-Mannosidase class II b (alpha-Man-IIb), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300412.1

ORF Insert Sequence:

ATGTCGTTCA AGCTATTCCG TCGCGGATCG GCGCGCTGCA TCGGTCTGCT ATCGGCGTTC 
GTAACGATCC TGCTCTGCCT CTACTACATC TCCATTGGCC AGCCCAATCC GACGCCCATT 
TCCGAGACGG GTGCCCCATC GGCGGCGTTA CGGGGATCAC CACTTAGTGG CCTAACTGGT 
TTCAGCGTCG ACGGAGGTTC GTCCTCGATC ATCCACCAAC AGAAGCAGCA ACTTGTGATC 
GCCTCAAACC AAAAGAAATC CTCGGAAACA AAGTCGGCGC AAACATCGGT ATCGCCGGAG 
GAGCAATCAG AGGAGCAGTA CACCAACAGC AATTGGGGCG ACAAGTGCTA CGAACTAATC 
CAGAGCAATA CAAACATAAC CGCTAGCGAG GAGCACAGCA AGTTTGACTT TCAGCCGGAA 
TGGATGCGAT CGAAGGAGTA CTGGGATCGT GGCTTTGAGG AGCGATTTGA TGCGCAGAAG 
AAGGACAAGC AGCGCCCGCC ACTAAAGATC ATAGTGGTGC CGCACTCCCA TAACGATCCT 
GGCTGGCTAA AGACCTTTAC CAACTACTTC CAATCGGATT CTCGGCAGAT TCTTAACCTA 
CTGGTGACCA AGATGCAGGA ATACACGGAC ATGACGTTCA TTTGGTCGGA GATCAGCTTC 
CTTCAGCTGT GGTGGGATCA AGCGCATCCA ACCAAGCAAC GGGCACTCAA GCGTTTAATC 
GACGCTGGTC GCATTGAAAT CACCACCGGT GGATGGGTGA TGACGGATGA GGCGAATGTC 
CACATCTATC CGATGTTAGA TCAGCTCATC GAGGGCCATC AGTGGCTAAA GAACAACCTG 
AATGTCACAC CGAAAGTGGG CTGGTCCATA GATCCATTTG GGCATGGCAG CACGGTGCCT 
TATCTGCTGT CCGGAGCGAA TTTCGAGGGA ACAATTATCC AGCGGATTCA TTACGCCTGG 
AAGCAGTGGT TTGCTCGCCA ACAGTCGGGT GACTTCATCT GGAAACCCTA TTGGAGAAGT 
AGAAAAGACA AGGATAGTGA AGCTGGTAGC CTTCTCACTC ACAACATGCC CTTCGATATA 
TACTCGATTA AGGGATCGTG TGGCCCACAT CCCTTCATAT GTCTGAACTT TGACTTTCGC 
AAAATTCCAG GCGAGTACAC CGAGTACTCC GTTAAGGCGC AGTTCATCAC AGACGACAAT 
CTGGAGTCAA AGGCGCAGTT GCTACTGGAG CAATATGCCA GGACTGCCTC GTTGTTTCCA 
CATAATGTGG CTCTCATTCC AGTCGGTGAT GATTTCCGAT ACAACAAGGA TCGCGAGGTG 
GATCAGCAGT ATCAGAACTA CAAGAAGCTT ATTGATCACA TCATGGCCAA TCGAAGGCTT 
TACAATGCTG ATATCCGATT TGGCACTCCC AGTGATTATT TCGAGGCCAT CAAGGAGCGC 
ATGAAGGATC ATACCCCAGG ATTTCCCACC TTTAAGGGTG ACTTCTTTGT TTATTCCGAT 
ATCTTTTCGG AGGGCAGACC GGCCTATTGG TCCGGTTACT TCACCACGCG TCCGTTCTAC 
AAGCTTCTGA GTTCAGAGCT GGAACATCAG CTGCGATCTG CTGAAATTGT CTTCACCCTG 
GCCTACAACA CAGCTCGACA AGCAAAGCGA GAGAATGCGA TAAAGATATA CGAGAAGAAC 
TACGAGCTTA TTATTAATGC AAGGCGAAAT TTGGGGCTGT TTCAGCACCA CGATGCCATC 
ACTGGTACAT CCAAGGCAGC GGTAATGAGG GACTATGCAA TGCGCCTGTT CGAGAGCACG 
CAAAACATGG TCAAGATGCA GGAATCCTGC CTAGAGCTCC TTCTACAAAA GGGCCAGCCT 
AGACATCACG GTTTCCTGCT CAGTGAATTT GAGCGAGATA ACTTCAACAA GCTGCCGCGC 
AAGATGCCGA TTCAGATGGC CAACGGGGAC GAGTCCACGC CCACTGTTCC GGCGGGAGAA 
GGCGGAGCCG GCGGATCATC TTCCAGCGGT TCGGTAGGCA GCTTTGTCCT GTACAATTCG 
CTAGCTCAGA AGCGGTTGGA AGTCGTCACG CTACGCGTCC AACATCCCAA TGTAAAGATA 
GTCAACGATA AGGGTGTAGA ATTGAATCAC ATCCAGATCA ATCCAGTGTG GAACATCACG 
GATACCTACG AACAGGGTCT GGGCACATCC GTATCCGGCA CTGTGGGTCG CATTCGCACT 
TCGACGCGCC AATTCGAGGT GATGTTCGTG GCGGAGCTGG AACCTTTATC GCTGAGCACT 
TATCGGGTAC AGGTGGACGA GGTCAACTTC AAGCGAAACA TAGCAACTAT ATACTGTGAC 
GATTGCACAG AATCGCAGCC AGCATCAGTG AGTCCGCCCG AAGAAATAGA ATCCTCACCG 
ACGTCAGTAT TTGAGGCGCG TGGCAAGCCA GCGGGCGATA TACAGTTGGA GAATCCTCAC 
ATGCGATTGC TGTTCGACGA GAAGAGCGGT TTTCTGAAGA CCATCACGCG TAAGAACCAG 
AAGAAGGAGT TGCTCAAGCC GCTTCAGTGC AACATCAAGT TTGCCGCTTA TCGCAGCGCC 
CAATTCCACT CTGGTGCATA TCTCTTCAAG ACCGATCCCG AACAAAGTGA GGCCGAGAAA 
GAAGTGCTTG AGGGCTATAC GGACATGCGC ATCATTATTA CCTCGGGTCC CATAGCCAGT 
GATGTGACTG TCATTTATGG ACCATTTTTG GCGCACACAG TGCGCATCTT CAACACGAGA 
ACTCATCTGG ATGCAGCGAT ATATGTTGAA AATGATATAG ATTTCGAGCC TCCACCCAAG 
AATCGGGAGA CGGAGCTGTT TATGCGATTT GTCACCAACA TAGACAATAT AGCTCAAGCT 
CCGATGCAGC GGGATCCGCT TAAGGAGCCG GTCGATGCGG CTACCATGCC GGAGCTTCCC 
GTCTTCTACA GCGATCAAAA CGGTTTCCAG TATCATGAAC GTATCAAAGT GCCGGCTATT 
GGCATCGAGG GCAACTACTT CCCGATCACC TCGGGAGCTT TTATTCAGGA CAGTCGCCTT 
CGCCTCACCC TGTTGACCAC TCATGCCCAA GGAGCAGCCA GTTATGAGCC TGGACAGCTG 
GAGGTTATGC TGGATCGGCG CACCCTGTAC GACGATTATC GGGGAATGGG CGAAGGCGTC 
GTGGACAGCC GTTTGACGCG GCACAAGTTC TGGCTGCTCG TGGAGGACCT CCCGGGTGGT 
CAGCATGTTA CGCAGCCACC GAGTTACAAG GTTCTCAGTC AGCAGGCTCA GCAGCTGGCA 
AATGCCTTGC GTTACCCGCC GAATCTGTAC TTTGTAAGCA ACCTGGAGCA GCCAGAACTG 
GCCGCTGCAT TGCTGCCTTT GGTCAGGCTT TTGCCTAAGG GACCGCTGCC CTGTGACGTA 
CACCTAACCA CCCTGCGTAC CCTATCCGAT CCGGAGTTGC AACTGTTTCC GTCCGCCTCG 
GCGCTGCTAG TTTTGCATCG CCAAGGATTC GATTGCAGTG TAAGCGCTGG CAGGGAATTG 
TCTATGGAGG CCATATGTCC CCTGTCCAGC AAGGGATTGG GTAATGTGCA GCTAGGTCGG 
CTCAGCCTGC ATACAATCGA AGCCACCAGC TTGACGGGGA CGGAGCAGAA ATCGTCCAGG 
GAGAGCTACC ACATACGCTC TCTGGCCGAG ATCAGTCTGG AACCAATGGA GCTACGCACC 
TTTAACCTTA CTTTCCGCTC GGAGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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alpha-Man-IIb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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