Trax cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trax
Entrez Gene ID	41871
Full Name	Translin associated factor X
Synonyms	CG5063,Dmel\CG5063,trax
General protein information	Preferred Names Translin associated factor X Names CG5063 gene product from transcript CG5063-RC CG5063-PB CG5063-PC Translin-associated factor X Trax-PB Trax-PC translin associated factor X
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88F1-88F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_169650.3	NP_732021.2	translin associated factor X, isoform B
NM_142197.5	NP_650454.2	translin associated factor X, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TSNAX	XP_514271.3
Canis lupus familiaris (dog)	TSNAX	XP_536345.2
Drosophila melanogaster (fruit fly)	Trax	NP_732021.2
Magnaporthe oryzae (rice blast fungus)	MGG_09242	XP_003709868.1
Rattus norvegicus (Norway rat)	Tsnax	NP_071598.1
Bos taurus (cattle)	TSNAX	NP_001069474.2
Danio rerio (zebrafish)	tsnax	NP_001025275.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004585	XP_315244.4
Homo sapiens (human)	TSNAX	NP_005990.1
Xenopus tropicalis (tropical clawed frog)	tsnax	NP_001006778.1
	TSNAX	XP_001104647.1
Mus musculus (house mouse)	Tsnax	NP_058605.1
Gallus gallus (chicken)	TSNAX	NP_001006403.1
Neurospora crassa	NCU06059	XP_959822.1
Arabidopsis thaliana (thale cress)	AT2G03780	NP_178473.2
Oryza sativa (rice)	Os01g0265900	NP_001042673.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These studies establish that Trax adopts the translin fold, possesses catalytic centers essential for C3PO's endoRNase activity and interacts extensively with translin to form an octameric assembly.
Title: Multimeric assembly and biochemical characterization of the Trax-translin endonuclease complex.

an RNAi regulator that we term C3PO (component 3 promoter of RISC), a complex of Translin and Trax was purified; C3PO is a Mg2+-dependent endoribonuclease that promotes RISC activation by removing siRNA passenger strand cleavage products
Title: C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation.

A translin null mutant was generated and a trax nonsense mutation was isolated. Translin is essential for stabilizing its interaction with trax.
Title: Functional characterization of Drosophila Translin and Trax.

The following Trax gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trax cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04992	NM_169650.3 Latest version!	Drosophila melanogaster translin associated factor X, transcript variant B (Trax), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa04992	NM_142197.5 Latest version!	Drosophila melanogaster translin associated factor X, transcript variant C (Trax), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trax ( NM_169650.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732021.2 Drosophila melanogaster translin associated factor X, transcript variant B (Trax), mRNA.

Price & Availability↑

CloneID	OFa04992
Clone ID Related Accession (Same CDS sequence)	NM_169650.3 , NM_142197.5
Accession Version	NM_169650.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	translin associated factor X, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169650.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAAA ACGGAGGTGC TGGTCACAGG AACACTGCTC CCAGGAAGCG CCAAATACCG 
GCTGCCCAGC TGGATGAAGA CAGTCCCATT GTCCAACAAT TTCGAATTTA TTCTAATGAA 
TTGATCATGA AACACGATCG CCACGAGCGA ATTGTCAAGC TGAGCCGTGA CATTACCATC 
GAGTCCAAGC GTATCATCTT CCTGCTGCAC TCCATTGACT CGCGTAAGCA GAATAAGGAG 
AAGGTCCTGG AGGAGGCACG TCAGCGACTG AACAAGCTGA TCGCGGTTAA CTTTAGGGCT 
GTGGCCCTGG AACTGCGCGA CCAGGATGTC TACCAGTTCC GCAGCTCCTA CTCTCCAGGA 
TTGCAGGAGT TTATCGAGGC CTACACCTAC ATGGAGTACC TGTGCCACGA GGATGCTGAG 
GGCGAGAACG AAACCAAGAG CGTTTCAGAT TGGCAGGCCA TTCAGGCGGT GATGCAGTAC 
GTCGAGGAGA GCAGCCAGCC AAAGGAAGAA CCAACTGAAG GCGAAGATGT TCAGGCTATA 
GCTCAGGTTG AGAGCCCAAA GAAGTTCCAG TTTTTCGTAG ACCCCACGGA ATATATACTT 
GGACTTTCGG ATCTTACTGG TGAACTGATG CGCCGCTGCA TAAATTCGCT AGGCAGTGGA 
GATACAGACA CCTGTTTGGA CACCTGCAAG GCCCTGCAGC ATTTCTACAG CGGTTACATC 
AGTCTAAACT GTCAGAGAGC CCGTGAACTA TGGCGTAAGA TCACTACCAT GAAGCAGAGC 
GTTCTTAAGG CCGAGAATGT TTGCTACAAT GTAAAAGTTC GCGGCGGAGA GGCTGCAAAA 
TGGGGCGCCA CTTTTGATCA AAAGCCAGCC GATGAAGTTG ACGAGGGCTT CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732021.2
CDS	134..1030
Translation	MPKNGGAGHRNTAPRKRQIPAAQLDEDSPIVQQFRIYSNELIMKHDRHERIVKLSRDITIESKRIIFLLHSIDSRKQNKEKVLEEARQRLNKLIAVNFRAVALELRDQDVYQFRSSYSPGLQEFIEAYTYMEYLCHEDAEGENETKSVSDWQAIQAVMQYVEESSQPKEEPTEGEDVQAIAQVESPKKFQFFVDPTEYILGLSDLTGELMRRCINSLGSGDTDTCLDTCKALQHFYSGYISLNCQRARELWRKITTMKQSVLKAENVCYNVKVRGGEAAKWGATFDQKPADEVDEGFY

Target ORF information:

RefSeq Version	NM_169650.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster translin associated factor X, transcript variant B (Trax), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169650.3

ORF Insert Sequence:

ATGCCGAAAA ACGGAGGTGC TGGTCACAGG AACACTGCTC CCAGGAAGCG CCAAATACCG 
GCTGCCCAGC TGGATGAAGA CAGTCCCATT GTCCAACAAT TTCGAATTTA TTCTAATGAA 
TTGATCATGA AACACGATCG CCACGAGCGA ATTGTCAAGC TGAGCCGTGA CATTACCATC 
GAGTCCAAGC GTATCATCTT CCTGCTGCAC TCCATTGACT CGCGTAAGCA GAATAAGGAG 
AAGGTCCTGG AGGAGGCACG TCAGCGACTG AACAAGCTGA TCGCGGTTAA CTTTAGGGCT 
GTGGCCCTGG AACTGCGCGA CCAGGATGTC TACCAGTTCC GCAGCTCCTA CTCTCCAGGA 
TTGCAGGAGT TTATCGAGGC CTACACCTAC ATGGAGTACC TGTGCCACGA GGATGCTGAG 
GGCGAGAACG AAACCAAGAG CGTTTCAGAT TGGCAGGCCA TTCAGGCGGT GATGCAGTAC 
GTCGAGGAGA GCAGCCAGCC AAAGGAAGAA CCAACTGAAG GCGAAGATGT TCAGGCTATA 
GCTCAGGTTG AGAGCCCAAA GAAGTTCCAG TTTTTCGTAG ACCCCACGGA ATATATACTT 
GGACTTTCGG ATCTTACTGG TGAACTGATG CGCCGCTGCA TAAATTCGCT AGGCAGTGGA 
GATACAGACA CCTGTTTGGA CACCTGCAAG GCCCTGCAGC ATTTCTACAG CGGTTACATC 
AGTCTAAACT GTCAGAGAGC CCGTGAACTA TGGCGTAAGA TCACTACCAT GAAGCAGAGC 
GTTCTTAAGG CCGAGAATGT TTGCTACAAT GTAAAAGTTC GCGGCGGAGA GGCTGCAAAA 
TGGGGCGCCA CTTTTGATCA AAAGCCAGCC GATGAAGTTG ACGAGGGCTT CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trax ( NM_142197.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650454.2 Drosophila melanogaster translin associated factor X, transcript variant C (Trax), mRNA.

Price & Availability↑

CloneID	OFa04992
Clone ID Related Accession (Same CDS sequence)	NM_169650.3 , NM_142197.5
Accession Version	NM_142197.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	translin associated factor X, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_142197.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAAA ACGGAGGTGC TGGTCACAGG AACACTGCTC CCAGGAAGCG CCAAATACCG 
GCTGCCCAGC TGGATGAAGA CAGTCCCATT GTCCAACAAT TTCGAATTTA TTCTAATGAA 
TTGATCATGA AACACGATCG CCACGAGCGA ATTGTCAAGC TGAGCCGTGA CATTACCATC 
GAGTCCAAGC GTATCATCTT CCTGCTGCAC TCCATTGACT CGCGTAAGCA GAATAAGGAG 
AAGGTCCTGG AGGAGGCACG TCAGCGACTG AACAAGCTGA TCGCGGTTAA CTTTAGGGCT 
GTGGCCCTGG AACTGCGCGA CCAGGATGTC TACCAGTTCC GCAGCTCCTA CTCTCCAGGA 
TTGCAGGAGT TTATCGAGGC CTACACCTAC ATGGAGTACC TGTGCCACGA GGATGCTGAG 
GGCGAGAACG AAACCAAGAG CGTTTCAGAT TGGCAGGCCA TTCAGGCGGT GATGCAGTAC 
GTCGAGGAGA GCAGCCAGCC AAAGGAAGAA CCAACTGAAG GCGAAGATGT TCAGGCTATA 
GCTCAGGTTG AGAGCCCAAA GAAGTTCCAG TTTTTCGTAG ACCCCACGGA ATATATACTT 
GGACTTTCGG ATCTTACTGG TGAACTGATG CGCCGCTGCA TAAATTCGCT AGGCAGTGGA 
GATACAGACA CCTGTTTGGA CACCTGCAAG GCCCTGCAGC ATTTCTACAG CGGTTACATC 
AGTCTAAACT GTCAGAGAGC CCGTGAACTA TGGCGTAAGA TCACTACCAT GAAGCAGAGC 
GTTCTTAAGG CCGAGAATGT TTGCTACAAT GTAAAAGTTC GCGGCGGAGA GGCTGCAAAA 
TGGGGCGCCA CTTTTGATCA AAAGCCAGCC GATGAAGTTG ACGAGGGCTT CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650454.2
CDS	134..1030
Translation	MPKNGGAGHRNTAPRKRQIPAAQLDEDSPIVQQFRIYSNELIMKHDRHERIVKLSRDITIESKRIIFLLHSIDSRKQNKEKVLEEARQRLNKLIAVNFRAVALELRDQDVYQFRSSYSPGLQEFIEAYTYMEYLCHEDAEGENETKSVSDWQAIQAVMQYVEESSQPKEEPTEGEDVQAIAQVESPKKFQFFVDPTEYILGLSDLTGELMRRCINSLGSGDTDTCLDTCKALQHFYSGYISLNCQRARELWRKITTMKQSVLKAENVCYNVKVRGGEAAKWGATFDQKPADEVDEGFY

Target ORF information:

RefSeq Version	NM_142197.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster translin associated factor X, transcript variant C (Trax), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142197.5

ORF Insert Sequence:

ATGCCGAAAA ACGGAGGTGC TGGTCACAGG AACACTGCTC CCAGGAAGCG CCAAATACCG 
GCTGCCCAGC TGGATGAAGA CAGTCCCATT GTCCAACAAT TTCGAATTTA TTCTAATGAA 
TTGATCATGA AACACGATCG CCACGAGCGA ATTGTCAAGC TGAGCCGTGA CATTACCATC 
GAGTCCAAGC GTATCATCTT CCTGCTGCAC TCCATTGACT CGCGTAAGCA GAATAAGGAG 
AAGGTCCTGG AGGAGGCACG TCAGCGACTG AACAAGCTGA TCGCGGTTAA CTTTAGGGCT 
GTGGCCCTGG AACTGCGCGA CCAGGATGTC TACCAGTTCC GCAGCTCCTA CTCTCCAGGA 
TTGCAGGAGT TTATCGAGGC CTACACCTAC ATGGAGTACC TGTGCCACGA GGATGCTGAG 
GGCGAGAACG AAACCAAGAG CGTTTCAGAT TGGCAGGCCA TTCAGGCGGT GATGCAGTAC 
GTCGAGGAGA GCAGCCAGCC AAAGGAAGAA CCAACTGAAG GCGAAGATGT TCAGGCTATA 
GCTCAGGTTG AGAGCCCAAA GAAGTTCCAG TTTTTCGTAG ACCCCACGGA ATATATACTT 
GGACTTTCGG ATCTTACTGG TGAACTGATG CGCCGCTGCA TAAATTCGCT AGGCAGTGGA 
GATACAGACA CCTGTTTGGA CACCTGCAAG GCCCTGCAGC ATTTCTACAG CGGTTACATC 
AGTCTAAACT GTCAGAGAGC CCGTGAACTA TGGCGTAAGA TCACTACCAT GAAGCAGAGC 
GTTCTTAAGG CCGAGAATGT TTGCTACAAT GTAAAAGTTC GCGGCGGAGA GGCTGCAAAA 
TGGGGCGCCA CTTTTGATCA AAAGCCAGCC GATGAAGTTG ACGAGGGCTT CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Trax cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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