Sra-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sra-1
Entrez Gene ID	41861
Full Name	specifically Rac1-associated protein 1
Synonyms	CG4931,CYFIP,CYFIP1/2,DSra-1,Dmel\CG4931,PIR121,PIR121/Sra-1,Sra,Sra-1/CYFIP,Sra1,Sra1/PIR121,cyfip,dSra,sra-1,sra1
General protein information	Preferred Names specifically Rac1-associated protein 1 Names CG4931 gene product from transcript CG4931-RA CG4931-PA Sra-1-PA specifically, Rac-1 associated protein 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88F1-88F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_142190.3	NP_650447.1	specifically Rac1-associated protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CYFIP2	XP_005619311.1
Danio rerio (zebrafish)	cyfip2	NP_001091056.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000195	XP_310939.3
Xenopus tropicalis (tropical clawed frog)	cyfip2	NP_001103523.1
	CYFIP2	XP_002804644.1
Bos taurus (cattle)	CYFIP2	NP_001192779.1
Drosophila melanogaster (fruit fly)	Sra-1	NP_650447.1
Arabidopsis thaliana (thale cress)	PIR121	NP_197342.3
Homo sapiens (human)	CYFIP2	NP_001032409.2
Pan troglodytes (chimpanzee)	CYFIP2	XP_001137208.1
Mus musculus (house mouse)	Cyfip2	NP_001239388.1
Gallus gallus (chicken)	CYFIP2	XP_004944971.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results illustrate a new model to explain the cellular roles of FMRP and CYFIP1 and the molecular significance of their interaction.
Title: New insights into the regulatory function of CYFIP1 in the context of WAVE- and FMRP-containing complexes.

We propose that Cyfip regulates synapse development and endocytosis by inhibiting actin assembly.
Title: Drosophila cyfip regulates synaptic development and endocytosis by suppressing filamentous actin assembly.

Mutations in WAVE/SCAR and in ARP2/3 complex subunits but not in WASP, another major regulator of Actin nucleation, phenocopy CYFIP defects. Thus, the CYFIP-SCAR-ARP2/3 pathway orchestrates specific tissue remodeling processes
Title: CYFIP dependent actin remodeling controls specific aspects of Drosophila eye morphogenesis.

Sra-1 function is mediated through kette. These stabilize each other and directly control the regulation of the cytoskeleton.
Title: Sra-1 interacts with Kette and Wasp and is required for neuronal and bristle development in Drosophila.

CYFIP specifically expressed in the nervous system; mutations affect axons and synapses, much like mutations in dFMR1 and Rho GTPase dRac1; CYFIP acts as a dRac1 effector that antagonizes FMR1 function
Title: CYFIP/Sra-1 controls neuronal connectivity in Drosophila and links the Rac1 GTPase pathway to the fragile X protein.

The following Sra-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sra-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04980	NM_142190.3 Latest version!	Drosophila melanogaster specifically Rac1-associated protein 1 (Sra-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sra-1 ( NM_142190.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650447.1 Drosophila melanogaster specifically Rac1-associated protein 1 (Sra-1), mRNA.

Price & Availability↑

CloneID	OFa04980
Clone ID Related Accession (Same CDS sequence)	NM_142190.3
Accession Version	NM_142190.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	specifically Rac1-associated protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142190.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGA AGATTACGCT AGCCGACGCG CTGTCCAACG TGGAGGTGTT GGACGAGCTA 
TCCCTGCCGG ACGAGCAGCC CTGCATCGAG GCGCAGCCCT GCTCGATTAT CTACAAGGCA 
AACTTCGATA CGAATTTCGA GGATCGCAAT GGATTTGTCA CGGGTATCGC CAAGTACATC 
GAGGAGGCCA CCACCCATGC CAACTTGAAT GTGCTCCTGG ACGAGGGGCA GAAACACGCA 
GTAATGCTCT ACACCTGGCG CTGCTGCTCG CGCGCCATTC CGCAGCCCAA GTCCAATGAG 
CAGCCGAATC GCGTGGAGAT CTACGAGAAG ACGGTCGAGG TGCTAGCCCC GGAGGTGAAC 
AAGCTGCTCA ACTTCATGTA CTTCCAACGC AAGGCCATCG AGGCCTTCTC CGGCGAGGTG 
AAGCGCTTGT GCCACGCCGA GAAGCGCAAG GACTTCGTGT CCGAGGCGTA CCTATTGACG 
CTAGGCAAGT TTATCAACAT GTTTGCCGTG CTGGACGAGC TGAAGAACAT GAAGTCCAGC 
GTGAAGAACG ATTACTCCAC CTACAGGCGA GCTGCGCAGT TCCTCAAAGT GATGTCGGAC 
TCGCACACCT TGCAGGAGTC GCAGAATCTG TCCATGTTCC TGGCCACGCA GAACAAGATT 
CGCGACACGG TCAAGGACAC GCTGGAGAAG ATCGTGGGCT ACGAGGATCT GCTCTCAGAT 
GTGGTCAACA TTTGCGTGCA TATGTTCGAG ACAAAGATGT ACTTGACCCC TGAGGAGAAG 
CACATGCTGG TAAAAGTTAT GGGCTTTGGC CTGTTCCTTA TGGACAGCGA TGCGTGCAAT 
ATCAACAAAC TTGATCAAAA GAAGAAGATA CGGCTGGATC GCATCGATCG CATCTTTAAG 
AACCTGGAAG TGGTGCCCCT CTTCGGCGAC ATGCAGATTG CTCCCTTCAA CTACATAAAG 
CGCAGCAAGC ACTTCGATTC CAGCAAGTGG CCGCTGTCCA GCTCAAACGC CATCAGCCCA 
CAGGCGGATC TAATGGTGCA TTTGCCGCAG ATACGCGAGG ATCACGTCAA GTACATTTCG 
GAGCTGGCGC GATACACAAA CGAGGTGACC ACCACCGTCA AGGAGAATCC CTCTGATGCC 
GAGAACCGGA TTACGGCCGA CCTGGCTCTG CGCGGCCTTC AATTGCTGTC CGAGTGGACC 
AGTGTGGTCA CCGAGTTGTA CTCCTGGAAG CTCCTGCATC CGACTGATCA TCACCAGAAC 
AAGGAGTGCC CCGTGGAGGC TGAGGAGTAC GAGCGGGCCA CCCGCTACAA TTACACCTCG 
GAAGAGAAGT TCGCGCTGAT CGAGGTCATC GCCATGATAA AGGGACTGCA GGTGCTAATG 
GCGCGCATTG AAACCGTATT GTGCGAGGCT ATCCGGCGTA ACATCTATTC CGAGCTGCAG 
GACTTCGTGC AGCTTTCGCT TCGTGAACCG CTGCGAAAGG CCGTAAAAAA TAAGAAGGAT 
CTTATCCGGA GCATCATCAT GTCGGTGCGA GAGACATCGG CGGACTGGCA AAAGGGCTAT 
GAGCCCACCG ACGATCCAGT GGCCAAGGGC AAGAAGGATC CCGACGGCGG CTTCCGCATC 
CAGGTGCCTC GCCTCAATGT GGGACCTTCC TCCACTCAGC TATACATGGT GCGCACCATG 
CTGGAGTCTC TGATTGCCGA CAAAAGCGGC GGCAAGCGCA CTTTGCGCAA GGACATCGAC 
GGAAACTGTT TGCTGCAGAT CGACACGTTC CACAAGACAT CGTTCTACTG GAGCTACTTG 
CTCAACTTCA GCGACACGCT TCAGAAGTGC TGCGATCTCT CGCAGCTGTG GTATCGCGAG 
TTCTACTTGG AGATGACCAT GGGTCGTAAG GTTAACAAGT GCTTGGTGCG GCATCAGCAC 
AACGAGGAGT GCAAGGATCT GATCACCATG GAGAAGCGCA TTCAGTTTCC GATTGAAATG 
TCCATGCCCT GGATCCTAAC CGATCACATT CTGCAAACGA AGGAGCCCTC AATGATGGAA 
TTTGTCTTGT ATCCCCTGGA CTTGTACAAC GACTCGGCGT ACTACGCACT CACTGTCTTC 
CGAAAGCAGT TCCTGTACGA CGAAGTGGAG GCCGAAGTCA ATCTGTGCTT CGATCAGTTT 
GTCTACAAGC TGAGCGAGCA GATCTTTGCC CATTACAAGC AATTGGCGGG CAGCATCTTC 
TTGGACAAGC GATTCCGTTT GGAGTGCGAG GTACTCGGTT TCAACTTCCA GTCGTATCCC 
CGCAATAATC GTTATGAGAC CTTGTTAAAA CAACGACATG TCCAACTTTT GGGCCGTTCT 
ATTGACTTGA ACAAACTGAT CACACAACGC ATAAACGCCA ACATGCACAA GAGCATTGAG 
TTGGCCATCA GTCGTTTCGA GGGCAACGAT ATAACCGGCA TTGTGGAACT CGAAGGCCTG 
CTAGAGGCGA ATCGAATTTG CCACAAGTTG CTAAGCAAGT ACTTGGCCCT GGATAACTTT 
GACGGCATGG TAAAGGAGGC TAACCACAAT GTACTGGCGC CTTACGGCAG AATAACACTG 
CACGTCTTTG TGGAATTGAA CTACGACTTT CTGGTCAATT ACTGCTATAA TGCAGCCACG 
AATCGTTTCA TTCGAACCAA AGTGAATCTT TCGTCGTCAC AGGCTATTCA GCGTGAGAAG 
CCGCCACAAA TGTCCCATTA CTATCTGTGG GGTTCCAAGC AGCTGAATGC TGCCTATTCC 
ACACAGTATG GCCAGTACAC TGGCTTTGTA GGTTCACCGC ATTTCCATGC CATGTGCCGC 
CTGCTGGGCT ATCAGGGCAT AGCAGTCGTC ATGGACATTA TACTGAAGGA CATTGTTAAG 
CCACTGATCC AGGGCTCGCT GCTGCAGTTC ACCAAGACGC TGATGATTGC CATGCCCAAG 
TCATGCAAAC TACCCCGTTG CGAATATGGT TCGCCTGGAG TGCTCAGCTA CTATCAGGCT 
CATCTTACAG ACATCGTGCA ATATCCGGAC GCGAAGACAG AGCTATTTCA GTCTTTCCGG 
GAGTTTGGCA ACAGCATTAT CTTTTGCCTG CTGATCGAAC AGGCGCTCTC GCAGGAGGAG 
GTGTGCGATC TACTGCACGC CGCCCTCTTC CAGAACATCT TTCCCAGACC TTTTTGCAAA 
GAGAATGAAA AACCGGAGGC TAAGCAAAAG CGTCTTGAAG CACAGTTTGC CAATCTGCAG 
ATAGTTTCAA ACGTGGAAAA GATTGGCACT GCAAAGCAAG CAATGATAGC ACGAGAGGGA 
GATTTGCTGA CTCGTGAGCG TCTTTGCTGT GGCCTCAGCA TTTTTGAAGT TATTCTCAAC 
CGCGTGAAGA GCTATTTGGA CGACCCTGTG TGGTGTGGTC CTCCACCGGC CAACGGGATT 
ATCCACGTGG ATGAGTGCTC GGAGTTCCAT CGCCTTTGGT CGGCTCTGCA GTTCGTTTAT 
TGCATTCCAG TCAGGGGAAC GGAGTACACC ATCGAGGAGC TCTTCGGCGA AGGTCTGAAC 
TGGGCTGGCT GCGTCATGAT CGTACTGCTG GGACAGCAGC GTCGCTTCGA GGCTCTGGAC 
TTTTGCTACC ACATTCTGCG GGTACAGCGC GTCGATGGCA AGGATGAGGA CGTAAAGGGC 
ATTCAATTGA AGCGAATGGT GGATCGGATT CGTCGGTTCC AAGTATTAAA CTCACAAATC 
TTCTCCATTC TCAACAAGTA TCTAAAGGGC GGCGATGGCG AGGGCTCAAA TGTGGAGCAC 
GTGCGGTGTT TTCCACCGCC TCAGCATCCC TCGGTCATAT CGTCCTCGTC GCACTACCAG 
GATCCCCAGA AGCTGCGGCA GAGCATAAAC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650447.1
CDS	124..3999
Translation	MTEKITLADALSNVEVLDELSLPDEQPCIEAQPCSIIYKANFDTNFEDRNGFVTGIAKYIEEATTHANLNVLLDEGQKHAVMLYTWRCCSRAIPQPKSNEQPNRVEIYEKTVEVLAPEVNKLLNFMYFQRKAIEAFSGEVKRLCHAEKRKDFVSEAYLLTLGKFINMFAVLDELKNMKSSVKNDYSTYRRAAQFLKVMSDSHTLQESQNLSMFLATQNKIRDTVKDTLEKIVGYEDLLSDVVNICVHMFETKMYLTPEEKHMLVKVMGFGLFLMDSDACNINKLDQKKKIRLDRIDRIFKNLEVVPLFGDMQIAPFNYIKRSKHFDSSKWPLSSSNAISPQADLMVHLPQIREDHVKYISELARYTNEVTTTVKENPSDAENRITADLALRGLQLLSEWTSVVTELYSWKLLHPTDHHQNKECPVEAEEYERATRYNYTSEEKFALIEVIAMIKGLQVLMARIETVLCEAIRRNIYSELQDFVQLSLREPLRKAVKNKKDLIRSIIMSVRETSADWQKGYEPTDDPVAKGKKDPDGGFRIQVPRLNVGPSSTQLYMVRTMLESLIADKSGGKRTLRKDIDGNCLLQIDTFHKTSFYWSYLLNFSDTLQKCCDLSQLWYREFYLEMTMGRKVNKCLVRHQHNEECKDLITMEKRIQFPIEMSMPWILTDHILQTKEPSMMEFVLYPLDLYNDSAYYALTVFRKQFLYDEVEAEVNLCFDQFVYKLSEQIFAHYKQLAGSIFLDKRFRLECEVLGFNFQSYPRNNRYETLLKQRHVQLLGRSIDLNKLITQRINANMHKSIELAISRFEGNDITGIVELEGLLEANRICHKLLSKYLALDNFDGMVKEANHNVLAPYGRITLHVFVELNYDFLVNYCYNAATNRFIRTKVNLSSSQAIQREKPPQMSHYYLWGSKQLNAAYSTQYGQYTGFVGSPHFHAMCRLLGYQGIAVVMDIILKDIVKPLIQGSLLQFTKTLMIAMPKSCKLPRCEYGSPGVLSYYQAHLTDIVQYPDAKTELFQSFREFGNSIIFCLLIEQALSQEEVCDLLHAALFQNIFPRPFCKENEKPEAKQKRLEAQFANLQIVSNVEKIGTAKQAMIAREGDLLTRERLCCGLSIFEVILNRVKSYLDDPVWCGPPPANGIIHVDECSEFHRLWSALQFVYCIPVRGTEYTIEELFGEGLNWAGCVMIVLLGQQRRFEALDFCYHILRVQRVDGKDEDVKGIQLKRMVDRIRRFQVLNSQIFSILNKYLKGGDGEGSNVEHVRCFPPPQHPSVISSSSHYQDPQKLRQSINN

Target ORF information:

RefSeq Version	NM_142190.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster specifically Rac1-associated protein 1 (Sra-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142190.3

ORF Insert Sequence:

ATGACGGAGA AGATTACGCT AGCCGACGCG CTGTCCAACG TGGAGGTGTT GGACGAGCTA 
TCCCTGCCGG ACGAGCAGCC CTGCATCGAG GCGCAGCCCT GCTCGATTAT CTACAAGGCA 
AACTTCGATA CGAATTTCGA GGATCGCAAT GGATTTGTCA CGGGTATCGC CAAGTACATC 
GAGGAGGCCA CCACCCATGC CAACTTGAAT GTGCTCCTGG ACGAGGGGCA GAAACACGCA 
GTAATGCTCT ACACCTGGCG CTGCTGCTCG CGCGCCATTC CGCAGCCCAA GTCCAATGAG 
CAGCCGAATC GCGTGGAGAT CTACGAGAAG ACGGTCGAGG TGCTAGCCCC GGAGGTGAAC 
AAGCTGCTCA ACTTCATGTA CTTCCAACGC AAGGCCATCG AGGCCTTCTC CGGCGAGGTG 
AAGCGCTTGT GCCACGCCGA GAAGCGCAAG GACTTCGTGT CCGAGGCGTA CCTATTGACG 
CTAGGCAAGT TTATCAACAT GTTTGCCGTG CTGGACGAGC TGAAGAACAT GAAGTCCAGC 
GTGAAGAACG ATTACTCCAC CTACAGGCGA GCTGCGCAGT TCCTCAAAGT GATGTCGGAC 
TCGCACACCT TGCAGGAGTC GCAGAATCTG TCCATGTTCC TGGCCACGCA GAACAAGATT 
CGCGACACGG TCAAGGACAC GCTGGAGAAG ATCGTGGGCT ACGAGGATCT GCTCTCAGAT 
GTGGTCAACA TTTGCGTGCA TATGTTCGAG ACAAAGATGT ACTTGACCCC TGAGGAGAAG 
CACATGCTGG TAAAAGTTAT GGGCTTTGGC CTGTTCCTTA TGGACAGCGA TGCGTGCAAT 
ATCAACAAAC TTGATCAAAA GAAGAAGATA CGGCTGGATC GCATCGATCG CATCTTTAAG 
AACCTGGAAG TGGTGCCCCT CTTCGGCGAC ATGCAGATTG CTCCCTTCAA CTACATAAAG 
CGCAGCAAGC ACTTCGATTC CAGCAAGTGG CCGCTGTCCA GCTCAAACGC CATCAGCCCA 
CAGGCGGATC TAATGGTGCA TTTGCCGCAG ATACGCGAGG ATCACGTCAA GTACATTTCG 
GAGCTGGCGC GATACACAAA CGAGGTGACC ACCACCGTCA AGGAGAATCC CTCTGATGCC 
GAGAACCGGA TTACGGCCGA CCTGGCTCTG CGCGGCCTTC AATTGCTGTC CGAGTGGACC 
AGTGTGGTCA CCGAGTTGTA CTCCTGGAAG CTCCTGCATC CGACTGATCA TCACCAGAAC 
AAGGAGTGCC CCGTGGAGGC TGAGGAGTAC GAGCGGGCCA CCCGCTACAA TTACACCTCG 
GAAGAGAAGT TCGCGCTGAT CGAGGTCATC GCCATGATAA AGGGACTGCA GGTGCTAATG 
GCGCGCATTG AAACCGTATT GTGCGAGGCT ATCCGGCGTA ACATCTATTC CGAGCTGCAG 
GACTTCGTGC AGCTTTCGCT TCGTGAACCG CTGCGAAAGG CCGTAAAAAA TAAGAAGGAT 
CTTATCCGGA GCATCATCAT GTCGGTGCGA GAGACATCGG CGGACTGGCA AAAGGGCTAT 
GAGCCCACCG ACGATCCAGT GGCCAAGGGC AAGAAGGATC CCGACGGCGG CTTCCGCATC 
CAGGTGCCTC GCCTCAATGT GGGACCTTCC TCCACTCAGC TATACATGGT GCGCACCATG 
CTGGAGTCTC TGATTGCCGA CAAAAGCGGC GGCAAGCGCA CTTTGCGCAA GGACATCGAC 
GGAAACTGTT TGCTGCAGAT CGACACGTTC CACAAGACAT CGTTCTACTG GAGCTACTTG 
CTCAACTTCA GCGACACGCT TCAGAAGTGC TGCGATCTCT CGCAGCTGTG GTATCGCGAG 
TTCTACTTGG AGATGACCAT GGGTCGTAAG GTTAACAAGT GCTTGGTGCG GCATCAGCAC 
AACGAGGAGT GCAAGGATCT GATCACCATG GAGAAGCGCA TTCAGTTTCC GATTGAAATG 
TCCATGCCCT GGATCCTAAC CGATCACATT CTGCAAACGA AGGAGCCCTC AATGATGGAA 
TTTGTCTTGT ATCCCCTGGA CTTGTACAAC GACTCGGCGT ACTACGCACT CACTGTCTTC 
CGAAAGCAGT TCCTGTACGA CGAAGTGGAG GCCGAAGTCA ATCTGTGCTT CGATCAGTTT 
GTCTACAAGC TGAGCGAGCA GATCTTTGCC CATTACAAGC AATTGGCGGG CAGCATCTTC 
TTGGACAAGC GATTCCGTTT GGAGTGCGAG GTACTCGGTT TCAACTTCCA GTCGTATCCC 
CGCAATAATC GTTATGAGAC CTTGTTAAAA CAACGACATG TCCAACTTTT GGGCCGTTCT 
ATTGACTTGA ACAAACTGAT CACACAACGC ATAAACGCCA ACATGCACAA GAGCATTGAG 
TTGGCCATCA GTCGTTTCGA GGGCAACGAT ATAACCGGCA TTGTGGAACT CGAAGGCCTG 
CTAGAGGCGA ATCGAATTTG CCACAAGTTG CTAAGCAAGT ACTTGGCCCT GGATAACTTT 
GACGGCATGG TAAAGGAGGC TAACCACAAT GTACTGGCGC CTTACGGCAG AATAACACTG 
CACGTCTTTG TGGAATTGAA CTACGACTTT CTGGTCAATT ACTGCTATAA TGCAGCCACG 
AATCGTTTCA TTCGAACCAA AGTGAATCTT TCGTCGTCAC AGGCTATTCA GCGTGAGAAG 
CCGCCACAAA TGTCCCATTA CTATCTGTGG GGTTCCAAGC AGCTGAATGC TGCCTATTCC 
ACACAGTATG GCCAGTACAC TGGCTTTGTA GGTTCACCGC ATTTCCATGC CATGTGCCGC 
CTGCTGGGCT ATCAGGGCAT AGCAGTCGTC ATGGACATTA TACTGAAGGA CATTGTTAAG 
CCACTGATCC AGGGCTCGCT GCTGCAGTTC ACCAAGACGC TGATGATTGC CATGCCCAAG 
TCATGCAAAC TACCCCGTTG CGAATATGGT TCGCCTGGAG TGCTCAGCTA CTATCAGGCT 
CATCTTACAG ACATCGTGCA ATATCCGGAC GCGAAGACAG AGCTATTTCA GTCTTTCCGG 
GAGTTTGGCA ACAGCATTAT CTTTTGCCTG CTGATCGAAC AGGCGCTCTC GCAGGAGGAG 
GTGTGCGATC TACTGCACGC CGCCCTCTTC CAGAACATCT TTCCCAGACC TTTTTGCAAA 
GAGAATGAAA AACCGGAGGC TAAGCAAAAG CGTCTTGAAG CACAGTTTGC CAATCTGCAG 
ATAGTTTCAA ACGTGGAAAA GATTGGCACT GCAAAGCAAG CAATGATAGC ACGAGAGGGA 
GATTTGCTGA CTCGTGAGCG TCTTTGCTGT GGCCTCAGCA TTTTTGAAGT TATTCTCAAC 
CGCGTGAAGA GCTATTTGGA CGACCCTGTG TGGTGTGGTC CTCCACCGGC CAACGGGATT 
ATCCACGTGG ATGAGTGCTC GGAGTTCCAT CGCCTTTGGT CGGCTCTGCA GTTCGTTTAT 
TGCATTCCAG TCAGGGGAAC GGAGTACACC ATCGAGGAGC TCTTCGGCGA AGGTCTGAAC 
TGGGCTGGCT GCGTCATGAT CGTACTGCTG GGACAGCAGC GTCGCTTCGA GGCTCTGGAC 
TTTTGCTACC ACATTCTGCG GGTACAGCGC GTCGATGGCA AGGATGAGGA CGTAAAGGGC 
ATTCAATTGA AGCGAATGGT GGATCGGATT CGTCGGTTCC AAGTATTAAA CTCACAAATC 
TTCTCCATTC TCAACAAGTA TCTAAAGGGC GGCGATGGCG AGGGCTCAAA TGTGGAGCAC 
GTGCGGTGTT TTCCACCGCC TCAGCATCCC TCGGTCATAT CGTCCTCGTC GCACTACCAG 
GATCCCCAGA AGCTGCGGCA GAGCATAAAC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sra-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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