Cp190 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cp190
Entrez Gene ID	41848
Full Name	Centrosomal protein 190kD
Synonyms	AbS1-4,Bx-63,Bx63,CEN190,CG6384,CP-190,CP-60,CP190,CP190_DROME,Cen185,Cen190,DMAP 190,DMAP-190,DMAP190,Dmap190,Dmel\CG6384,E(mod)4-1,Map190,Rb188,cp190,dmap190
General protein information	Preferred Names Centrosomal protein 190kD Names CG6384 gene product from transcript CG6384-RA CG6384-PA CG6384-PB Cp190-PA Cp190-PB E(mod)4-1 centrosomal protein 190 centrosomal protein 190 kD centrosomal protein 190 kDa centrosomal protein 190kD centrosomal protein 90 centrosomal protein of 190 kDa enhancer of mod(mdg4)4-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88E9-88E11

mRNA and Protein(s)

mRNA	Protein	Name
NM_079635.3	NP_524359.2	centrosomal protein 190kD, isoform A
NM_169632.2	NP_731998.1	centrosomal protein 190kD, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results support the model that CP190 recruits Gcn5 and thereby activates chromatin.
Title: Chromatin binding of Gcn5 in Drosophila is largely mediated by CP190.

Drosophila CTCF-CP190 interactions are less critical for the in vivo functions of the CTCF protein than the N-terminal CTCF - CTCF interaction domain.
Title: Functional role of dimerization and CP190 interacting domains of CTCF protein in Drosophila melanogaster.

As binding of CP190 does not enhance luciferase activity in reporter assays.
Title: Ectopically tethered CP190 induces large-scale chromatin decondensation.

Results show that CP190 binds to CTCF or to other DNA binding insulator factors serving as a binding platform for compexes with enzymatic function, like NURF and dREAM.
Title: A functional insulator screen identifies NURF and dREAM components to be required for enhancer-blocking.

we discuss the emerging role of CP190 in tethering genome, specifically in the perspective of insulator function in Drosophila.
Title: Functional sub-division of the Drosophila genome via chromatin looping: the emerging importance of CP190.

The following Cp190 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cp190 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04960	NM_169632.2 Latest version!	Drosophila melanogaster centrosomal protein 190kD, transcript variant B (Cp190), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa04960	NM_079635.3 Latest version!	Drosophila melanogaster centrosomal protein 190kD, transcript variant A (Cp190), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cp190 ( NM_079635.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524359.2 Drosophila melanogaster centrosomal protein 190kD, transcript variant A (Cp190), mRNA.

Price & Availability↑

CloneID	OFa04960
Clone ID Related Accession (Same CDS sequence)	NM_079635.3 , NM_169632.2
Accession Version	NM_079635.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	centrosomal protein 190kD, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079635.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTGAAG TCAAGTCCGT GAAAGTGGAC AACTGGGGAG TCTTCTTCCT GCAGAAGCTG 
CAGAACTTCT TTAATAAAAC GGATTACTGC GATTTGACGC TGCAGTTTCG GGACAATTCA 
CAGCTAAAGG TACACCGTCT GGTGCTCAGC GCCTGCACCG ACTACTTCAA TGTACTGGAG 
CAGACCTGCG AGATCGTCGA CGATGCCCTC ATCATGCCCA ACGAGTTCCA GGCGGACGTG 
GTGGTGCCCA TTGTCAACTT CATGTACACA GGCACCCTGG AGTTTGAGCT TAAAATGTAC 
GGCAAGCTGT TGCGCACCGC CAAGGAAATG AACATGACGG TGCTGTTGAA GCTGCTAGAG 
GCGCACCGTC GCACCATGGA GAACGTTAAT CGCCAGCAAA GGCCGCCTAG TCCAAAGGGA 
ATACGCCGGC GCACAGTTGG ACAGCCCAGT TCGGGTCTTC CACAACAGCG CGTCTTGGGT 
CCTTCGCCGC AATCGCGAAA CGTAGCCACA CCCATCGCGC AACGAGCCAA CACACAGCGC 
GGGTCAACTG GCAACACAAT GAGCCGAACA TCAGGTGGCT CAAATCGTTC GCCGTATGGA 
GATAGTAGCA ATGTGAAGCA GGAGCCAACG TCACCATTCG AGCAGCTGCG AAAGGGTTAT 
AACAATAACA AGAGACCAGC ACAGACAAGC CTGCTATCGC CGCCATCCAA GAAGCCCAGT 
TTGGAAGAGG TTAAGGAATT CGCTGAGCAA CAACGGATGC GAAAGCAAAT CGCCGCTGAG 
TACGGAGATA ACGATCCTGA GTACGATGGA GGAATGCTTT ATGATGATGT CCATGCCGGG 
GACGATGATG ACGATGATAT GCCACCGCAG CCATCTACAT CTAAGCAGCA ATCCCCACAG 
GGTACACAGA CCCAACTAGA ACATGGATCC ACCACCATTA TCTTGAAACA GGACTCACCC 
AGCCAGACCC CAACAATAAT TGTCAAGGAC TCCTCCAACG CCAAGCTGAA TCACACCAAG 
ATCATTGCCG AAGTCTTGCG CCAGTATCCG CACATTGTAA AGGGCCACAA AAACATAAAG 
CTAAAGATTA TGCCCAATAC CCCGGCAGCG CCGACCGAAA AATCTGCTCC AGCGACAGTA 
AAACCCCCGG CGAACCAGTC ATCCGCAACT ACATCGCCAC ACAAGAAACT GCACGTTTCT 
TTCAAAGCCG ACAAGAGTAC GCCACTAATC ACAGCACAAC AGAAGGCGGC TAGCTCTCAG 
CAAAAGTCGG GAACCTCGCA GACAACCGGT AACCAGGGAA CCGGGGCGAA TCCACCAGCA 
AATACAGCCG CAGCCCAGAA GAGACGCATT GATAGTAAGA CAATGCACGC CTTGATCGCT 
CAGGGAGCAG AGAATACTAC TGGGCCCTGG CTGTGCCTGA GATGCGGCGT AAACGGACGA 
CCCATTAGCA TTCCGTCGTA CCGAGGCTTC CGACGCCATC TGATCAACAC GCACAAGGAG 
ACAATTGATC CGGCGCTTTG TGAGCATTGC GGGTGGAGGT CTGTCAACAA CCGGGAACTG 
CATTTCCACA TGTATATGGA GCACCAGACC AAGTCGCTGC TGTATACGTT TGCCGAGTGC 
GCGCTATGCA ACCAGTCTTA CCGGACCAAG GGTGAGTTGG AGGCGCACAT AAACGAGGTG 
CACACGGACG ACAACAAGCA GCAGTGCATC TACTGCAACA AGGTGTTCGA GCAAGAGCTG 
CAGCTGTACA GACACATGAA GAGCTACCAC AAGGAGCAGG CTTTGGAGGA TGGCATTATC 
GATGAAACGG ACGAAGAATT CCTCGGCTCG CAAGACGAGG AGGAAGAGGC CGAAGGAGAC 
GAAGAGCAGG AACCAGAGCA GACTGGCAAA GTGCGAATCC TTTCGGACAT AAGTCTACCA 
GCCACCAGTG CCATCACAGT GCAGCAAGCT CAACAGGAGC AACTGCAAGA GGAAGATGTT 
GAACAGGTGC AGCAGGAAGT CAAATTCGTG GGCGCCGATG GCAATGAGGT GGAGCTTACT 
GATGAGCAGC GCAAAGAGAT TCTCTCACAG CTTAACCAGC AGCAAGCAGG CGCCACTGCC 
GGCGGTGTGG TAATGGTGCT CAGTGAGCCA GAAGCGGAGC ACGTCAAGCA GGAGACCGAT 
GAAAAATCCC TGGCCGGCAC CGAAGAGGAA TATGATGACA GTCAAATCTA TAGCGAACTG 
GGTGCTGCCG ACTCTGTGGA GAGCGCCAAA AAGAACATTG CTGATGAAAG CAAAGAGTCC 
ATTGACAACC TGGAATGGGC CGAAAACCTT ATAGCCGAGT CTGAAGAACA GAGCAACAAG 
GAGCCAAAAT CGGATAAGCC GCGAGATGAC ATTAGTGAAA AGCTAAAGGA GCTTACCGGC 
GACTGGACGG AGGATGAGAA CGATGACGAT GTCGACGATA AGCCCGCTAC CGCAGAGCTT 
GCATCCGAAC TAGCCAACAA GGACCCTGAG CCAACAGTAC ACGAGGAAGA AGATGATATT 
GACTTGGCTC TCCAGTCTCT ACACAAGGGA CCCGAAGAGG CAACTGAAGA AAAGGCAAGT 
GAGGAGTCTG TCACCAGTGC GGACGATGCA GTTGACGCAG TCCCCAATAT AAATAGTCAG 
CCCGAGAAAA TGGATGTGGA CTCAGAAGCG GCGGACGAGA AGGCTTCGAA GGCCGAGGTC 
CAGATCAAGA AAGAGGCTGA GCTTGAAAAC GATCAAGAGG AATTTATCAA AGAGGATTCA 
CCAATTCCGC ATTCCGATTC CGTGGCGGAA TTAAGAGAGG CGGTAACTGC CTCTGAGGGC 
GAGGATGATG TGCATCTAGA GGCCGATAAC ATACGCAAAG AGCTGCTAGA TGAGTTAATT 
GCCGAGGCCG AGAAACCTGA TCAGGAGAAG GACATTGTTC AATCTGAAGA GAATGCGACG 
ACAGAGGCGT TAGATAGGTC AGTAACGGAC GAGGATGACC TGGTGCCACC TACGCAGGTA 
TCTACCGAAC AAATGGAGAT TGATGAACCA GCAGCGGAAA AAGCAGCCGA AAATAATGAA 
GATACTAGAA CGGCAGACGA GAAGGAAGCC GTCGAGGACA AACCAAACCA GACACAGGAT 
GTAACCACTG CAGAAAAGCC GACACTAGAA AGTGCAAAAG CAGGTGATGA AGCAACGTCA 
GGTGAAGCAG CCAGCGTGGA CAAGGTCAAG AGCCTCATTA GCGAATGGGG TGACGACGAT 
GAGGACGAGG ATGAGAATGG GGTTAGTGCG GCGGCGAAGG AGGAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524359.2
CDS	213..3503
Translation	MGEVKSVKVDNWGVFFLQKLQNFFNKTDYCDLTLQFRDNSQLKVHRLVLSACTDYFNVLEQTCEIVDDALIMPNEFQADVVVPIVNFMYTGTLEFELKMYGKLLRTAKEMNMTVLLKLLEAHRRTMENVNRQQRPPSPKGIRRRTVGQPSSGLPQQRVLGPSPQSRNVATPIAQRANTQRGSTGNTMSRTSGGSNRSPYGDSSNVKQEPTSPFEQLRKGYNNNKRPAQTSLLSPPSKKPSLEEVKEFAEQQRMRKQIAAEYGDNDPEYDGGMLYDDVHAGDDDDDDMPPQPSTSKQQSPQGTQTQLEHGSTTIILKQDSPSQTPTIIVKDSSNAKLNHTKIIAEVLRQYPHIVKGHKNIKLKIMPNTPAAPTEKSAPATVKPPANQSSATTSPHKKLHVSFKADKSTPLITAQQKAASSQQKSGTSQTTGNQGTGANPPANTAAAQKRRIDSKTMHALIAQGAENTTGPWLCLRCGVNGRPISIPSYRGFRRHLINTHKETIDPALCEHCGWRSVNNRELHFHMYMEHQTKSLLYTFAECALCNQSYRTKGELEAHINEVHTDDNKQQCIYCNKVFEQELQLYRHMKSYHKEQALEDGIIDETDEEFLGSQDEEEEAEGDEEQEPEQTGKVRILSDISLPATSAITVQQAQQEQLQEEDVEQVQQEVKFVGADGNEVELTDEQRKEILSQLNQQQAGATAGGVVMVLSEPEAEHVKQETDEKSLAGTEEEYDDSQIYSELGAADSVESAKKNIADESKESIDNLEWAENLIAESEEQSNKEPKSDKPRDDISEKLKELTGDWTEDENDDDVDDKPATAELASELANKDPEPTVHEEEDDIDLALQSLHKGPEEATEEKASEESVTSADDAVDAVPNINSQPEKMDVDSEAADEKASKAEVQIKKEAELENDQEEFIKEDSPIPHSDSVAELREAVTASEGEDDVHLEADNIRKELLDELIAEAEKPDQEKDIVQSEENATTEALDRSVTDEDDLVPPTQVSTEQMEIDEPAAEKAAENNEDTRTADEKEAVEDKPNQTQDVTTAEKPTLESAKAGDEATSGEAASVDKVKSLISEWGDDDEDEDENGVSAAAKEEL

Target ORF information:

RefSeq Version	NM_079635.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster centrosomal protein 190kD, transcript variant A (Cp190), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079635.3

ORF Insert Sequence:

ATGGGTGAAG TCAAGTCCGT GAAAGTGGAC AACTGGGGAG TCTTCTTCCT GCAGAAGCTG 
CAGAACTTCT TTAATAAAAC GGATTACTGC GATTTGACGC TGCAGTTTCG GGACAATTCA 
CAGCTAAAGG TACACCGTCT GGTGCTCAGC GCCTGCACCG ACTACTTCAA TGTACTGGAG 
CAGACCTGCG AGATCGTCGA CGATGCCCTC ATCATGCCCA ACGAGTTCCA GGCGGACGTG 
GTGGTGCCCA TTGTCAACTT CATGTACACA GGCACCCTGG AGTTTGAGCT TAAAATGTAC 
GGCAAGCTGT TGCGCACCGC CAAGGAAATG AACATGACGG TGCTGTTGAA GCTGCTAGAG 
GCGCACCGTC GCACCATGGA GAACGTTAAT CGCCAGCAAA GGCCGCCTAG TCCAAAGGGA 
ATACGCCGGC GCACAGTTGG ACAGCCCAGT TCGGGTCTTC CACAACAGCG CGTCTTGGGT 
CCTTCGCCGC AATCGCGAAA CGTAGCCACA CCCATCGCGC AACGAGCCAA CACACAGCGC 
GGGTCAACTG GCAACACAAT GAGCCGAACA TCAGGTGGCT CAAATCGTTC GCCGTATGGA 
GATAGTAGCA ATGTGAAGCA GGAGCCAACG TCACCATTCG AGCAGCTGCG AAAGGGTTAT 
AACAATAACA AGAGACCAGC ACAGACAAGC CTGCTATCGC CGCCATCCAA GAAGCCCAGT 
TTGGAAGAGG TTAAGGAATT CGCTGAGCAA CAACGGATGC GAAAGCAAAT CGCCGCTGAG 
TACGGAGATA ACGATCCTGA GTACGATGGA GGAATGCTTT ATGATGATGT CCATGCCGGG 
GACGATGATG ACGATGATAT GCCACCGCAG CCATCTACAT CTAAGCAGCA ATCCCCACAG 
GGTACACAGA CCCAACTAGA ACATGGATCC ACCACCATTA TCTTGAAACA GGACTCACCC 
AGCCAGACCC CAACAATAAT TGTCAAGGAC TCCTCCAACG CCAAGCTGAA TCACACCAAG 
ATCATTGCCG AAGTCTTGCG CCAGTATCCG CACATTGTAA AGGGCCACAA AAACATAAAG 
CTAAAGATTA TGCCCAATAC CCCGGCAGCG CCGACCGAAA AATCTGCTCC AGCGACAGTA 
AAACCCCCGG CGAACCAGTC ATCCGCAACT ACATCGCCAC ACAAGAAACT GCACGTTTCT 
TTCAAAGCCG ACAAGAGTAC GCCACTAATC ACAGCACAAC AGAAGGCGGC TAGCTCTCAG 
CAAAAGTCGG GAACCTCGCA GACAACCGGT AACCAGGGAA CCGGGGCGAA TCCACCAGCA 
AATACAGCCG CAGCCCAGAA GAGACGCATT GATAGTAAGA CAATGCACGC CTTGATCGCT 
CAGGGAGCAG AGAATACTAC TGGGCCCTGG CTGTGCCTGA GATGCGGCGT AAACGGACGA 
CCCATTAGCA TTCCGTCGTA CCGAGGCTTC CGACGCCATC TGATCAACAC GCACAAGGAG 
ACAATTGATC CGGCGCTTTG TGAGCATTGC GGGTGGAGGT CTGTCAACAA CCGGGAACTG 
CATTTCCACA TGTATATGGA GCACCAGACC AAGTCGCTGC TGTATACGTT TGCCGAGTGC 
GCGCTATGCA ACCAGTCTTA CCGGACCAAG GGTGAGTTGG AGGCGCACAT AAACGAGGTG 
CACACGGACG ACAACAAGCA GCAGTGCATC TACTGCAACA AGGTGTTCGA GCAAGAGCTG 
CAGCTGTACA GACACATGAA GAGCTACCAC AAGGAGCAGG CTTTGGAGGA TGGCATTATC 
GATGAAACGG ACGAAGAATT CCTCGGCTCG CAAGACGAGG AGGAAGAGGC CGAAGGAGAC 
GAAGAGCAGG AACCAGAGCA GACTGGCAAA GTGCGAATCC TTTCGGACAT AAGTCTACCA 
GCCACCAGTG CCATCACAGT GCAGCAAGCT CAACAGGAGC AACTGCAAGA GGAAGATGTT 
GAACAGGTGC AGCAGGAAGT CAAATTCGTG GGCGCCGATG GCAATGAGGT GGAGCTTACT 
GATGAGCAGC GCAAAGAGAT TCTCTCACAG CTTAACCAGC AGCAAGCAGG CGCCACTGCC 
GGCGGTGTGG TAATGGTGCT CAGTGAGCCA GAAGCGGAGC ACGTCAAGCA GGAGACCGAT 
GAAAAATCCC TGGCCGGCAC CGAAGAGGAA TATGATGACA GTCAAATCTA TAGCGAACTG 
GGTGCTGCCG ACTCTGTGGA GAGCGCCAAA AAGAACATTG CTGATGAAAG CAAAGAGTCC 
ATTGACAACC TGGAATGGGC CGAAAACCTT ATAGCCGAGT CTGAAGAACA GAGCAACAAG 
GAGCCAAAAT CGGATAAGCC GCGAGATGAC ATTAGTGAAA AGCTAAAGGA GCTTACCGGC 
GACTGGACGG AGGATGAGAA CGATGACGAT GTCGACGATA AGCCCGCTAC CGCAGAGCTT 
GCATCCGAAC TAGCCAACAA GGACCCTGAG CCAACAGTAC ACGAGGAAGA AGATGATATT 
GACTTGGCTC TCCAGTCTCT ACACAAGGGA CCCGAAGAGG CAACTGAAGA AAAGGCAAGT 
GAGGAGTCTG TCACCAGTGC GGACGATGCA GTTGACGCAG TCCCCAATAT AAATAGTCAG 
CCCGAGAAAA TGGATGTGGA CTCAGAAGCG GCGGACGAGA AGGCTTCGAA GGCCGAGGTC 
CAGATCAAGA AAGAGGCTGA GCTTGAAAAC GATCAAGAGG AATTTATCAA AGAGGATTCA 
CCAATTCCGC ATTCCGATTC CGTGGCGGAA TTAAGAGAGG CGGTAACTGC CTCTGAGGGC 
GAGGATGATG TGCATCTAGA GGCCGATAAC ATACGCAAAG AGCTGCTAGA TGAGTTAATT 
GCCGAGGCCG AGAAACCTGA TCAGGAGAAG GACATTGTTC AATCTGAAGA GAATGCGACG 
ACAGAGGCGT TAGATAGGTC AGTAACGGAC GAGGATGACC TGGTGCCACC TACGCAGGTA 
TCTACCGAAC AAATGGAGAT TGATGAACCA GCAGCGGAAA AAGCAGCCGA AAATAATGAA 
GATACTAGAA CGGCAGACGA GAAGGAAGCC GTCGAGGACA AACCAAACCA GACACAGGAT 
GTAACCACTG CAGAAAAGCC GACACTAGAA AGTGCAAAAG CAGGTGATGA AGCAACGTCA 
GGTGAAGCAG CCAGCGTGGA CAAGGTCAAG AGCCTCATTA GCGAATGGGG TGACGACGAT 
GAGGACGAGG ATGAGAATGG GGTTAGTGCG GCGGCGAAGG AGGAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cp190 ( NM_169632.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731998.1 Drosophila melanogaster centrosomal protein 190kD, transcript variant B (Cp190), mRNA.

Price & Availability↑

CloneID	OFa04960
Clone ID Related Accession (Same CDS sequence)	NM_079635.3 , NM_169632.2
Accession Version	NM_169632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	centrosomal protein 190kD, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169632.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTGAAG TCAAGTCCGT GAAAGTGGAC AACTGGGGAG TCTTCTTCCT GCAGAAGCTG 
CAGAACTTCT TTAATAAAAC GGATTACTGC GATTTGACGC TGCAGTTTCG GGACAATTCA 
CAGCTAAAGG TACACCGTCT GGTGCTCAGC GCCTGCACCG ACTACTTCAA TGTACTGGAG 
CAGACCTGCG AGATCGTCGA CGATGCCCTC ATCATGCCCA ACGAGTTCCA GGCGGACGTG 
GTGGTGCCCA TTGTCAACTT CATGTACACA GGCACCCTGG AGTTTGAGCT TAAAATGTAC 
GGCAAGCTGT TGCGCACCGC CAAGGAAATG AACATGACGG TGCTGTTGAA GCTGCTAGAG 
GCGCACCGTC GCACCATGGA GAACGTTAAT CGCCAGCAAA GGCCGCCTAG TCCAAAGGGA 
ATACGCCGGC GCACAGTTGG ACAGCCCAGT TCGGGTCTTC CACAACAGCG CGTCTTGGGT 
CCTTCGCCGC AATCGCGAAA CGTAGCCACA CCCATCGCGC AACGAGCCAA CACACAGCGC 
GGGTCAACTG GCAACACAAT GAGCCGAACA TCAGGTGGCT CAAATCGTTC GCCGTATGGA 
GATAGTAGCA ATGTGAAGCA GGAGCCAACG TCACCATTCG AGCAGCTGCG AAAGGGTTAT 
AACAATAACA AGAGACCAGC ACAGACAAGC CTGCTATCGC CGCCATCCAA GAAGCCCAGT 
TTGGAAGAGG TTAAGGAATT CGCTGAGCAA CAACGGATGC GAAAGCAAAT CGCCGCTGAG 
TACGGAGATA ACGATCCTGA GTACGATGGA GGAATGCTTT ATGATGATGT CCATGCCGGG 
GACGATGATG ACGATGATAT GCCACCGCAG CCATCTACAT CTAAGCAGCA ATCCCCACAG 
GGTACACAGA CCCAACTAGA ACATGGATCC ACCACCATTA TCTTGAAACA GGACTCACCC 
AGCCAGACCC CAACAATAAT TGTCAAGGAC TCCTCCAACG CCAAGCTGAA TCACACCAAG 
ATCATTGCCG AAGTCTTGCG CCAGTATCCG CACATTGTAA AGGGCCACAA AAACATAAAG 
CTAAAGATTA TGCCCAATAC CCCGGCAGCG CCGACCGAAA AATCTGCTCC AGCGACAGTA 
AAACCCCCGG CGAACCAGTC ATCCGCAACT ACATCGCCAC ACAAGAAACT GCACGTTTCT 
TTCAAAGCCG ACAAGAGTAC GCCACTAATC ACAGCACAAC AGAAGGCGGC TAGCTCTCAG 
CAAAAGTCGG GAACCTCGCA GACAACCGGT AACCAGGGAA CCGGGGCGAA TCCACCAGCA 
AATACAGCCG CAGCCCAGAA GAGACGCATT GATAGTAAGA CAATGCACGC CTTGATCGCT 
CAGGGAGCAG AGAATACTAC TGGGCCCTGG CTGTGCCTGA GATGCGGCGT AAACGGACGA 
CCCATTAGCA TTCCGTCGTA CCGAGGCTTC CGACGCCATC TGATCAACAC GCACAAGGAG 
ACAATTGATC CGGCGCTTTG TGAGCATTGC GGGTGGAGGT CTGTCAACAA CCGGGAACTG 
CATTTCCACA TGTATATGGA GCACCAGACC AAGTCGCTGC TGTATACGTT TGCCGAGTGC 
GCGCTATGCA ACCAGTCTTA CCGGACCAAG GGTGAGTTGG AGGCGCACAT AAACGAGGTG 
CACACGGACG ACAACAAGCA GCAGTGCATC TACTGCAACA AGGTGTTCGA GCAAGAGCTG 
CAGCTGTACA GACACATGAA GAGCTACCAC AAGGAGCAGG CTTTGGAGGA TGGCATTATC 
GATGAAACGG ACGAAGAATT CCTCGGCTCG CAAGACGAGG AGGAAGAGGC CGAAGGAGAC 
GAAGAGCAGG AACCAGAGCA GACTGGCAAA GTGCGAATCC TTTCGGACAT AAGTCTACCA 
GCCACCAGTG CCATCACAGT GCAGCAAGCT CAACAGGAGC AACTGCAAGA GGAAGATGTT 
GAACAGGTGC AGCAGGAAGT CAAATTCGTG GGCGCCGATG GCAATGAGGT GGAGCTTACT 
GATGAGCAGC GCAAAGAGAT TCTCTCACAG CTTAACCAGC AGCAAGCAGG CGCCACTGCC 
GGCGGTGTGG TAATGGTGCT CAGTGAGCCA GAAGCGGAGC ACGTCAAGCA GGAGACCGAT 
GAAAAATCCC TGGCCGGCAC CGAAGAGGAA TATGATGACA GTCAAATCTA TAGCGAACTG 
GGTGCTGCCG ACTCTGTGGA GAGCGCCAAA AAGAACATTG CTGATGAAAG CAAAGAGTCC 
ATTGACAACC TGGAATGGGC CGAAAACCTT ATAGCCGAGT CTGAAGAACA GAGCAACAAG 
GAGCCAAAAT CGGATAAGCC GCGAGATGAC ATTAGTGAAA AGCTAAAGGA GCTTACCGGC 
GACTGGACGG AGGATGAGAA CGATGACGAT GTCGACGATA AGCCCGCTAC CGCAGAGCTT 
GCATCCGAAC TAGCCAACAA GGACCCTGAG CCAACAGTAC ACGAGGAAGA AGATGATATT 
GACTTGGCTC TCCAGTCTCT ACACAAGGGA CCCGAAGAGG CAACTGAAGA AAAGGCAAGT 
GAGGAGTCTG TCACCAGTGC GGACGATGCA GTTGACGCAG TCCCCAATAT AAATAGTCAG 
CCCGAGAAAA TGGATGTGGA CTCAGAAGCG GCGGACGAGA AGGCTTCGAA GGCCGAGGTC 
CAGATCAAGA AAGAGGCTGA GCTTGAAAAC GATCAAGAGG AATTTATCAA AGAGGATTCA 
CCAATTCCGC ATTCCGATTC CGTGGCGGAA TTAAGAGAGG CGGTAACTGC CTCTGAGGGC 
GAGGATGATG TGCATCTAGA GGCCGATAAC ATACGCAAAG AGCTGCTAGA TGAGTTAATT 
GCCGAGGCCG AGAAACCTGA TCAGGAGAAG GACATTGTTC AATCTGAAGA GAATGCGACG 
ACAGAGGCGT TAGATAGGTC AGTAACGGAC GAGGATGACC TGGTGCCACC TACGCAGGTA 
TCTACCGAAC AAATGGAGAT TGATGAACCA GCAGCGGAAA AAGCAGCCGA AAATAATGAA 
GATACTAGAA CGGCAGACGA GAAGGAAGCC GTCGAGGACA AACCAAACCA GACACAGGAT 
GTAACCACTG CAGAAAAGCC GACACTAGAA AGTGCAAAAG CAGGTGATGA AGCAACGTCA 
GGTGAAGCAG CCAGCGTGGA CAAGGTCAAG AGCCTCATTA GCGAATGGGG TGACGACGAT 
GAGGACGAGG ATGAGAATGG GGTTAGTGCG GCGGCGAAGG AGGAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731998.1
CDS	275..3565
Translation	MGEVKSVKVDNWGVFFLQKLQNFFNKTDYCDLTLQFRDNSQLKVHRLVLSACTDYFNVLEQTCEIVDDALIMPNEFQADVVVPIVNFMYTGTLEFELKMYGKLLRTAKEMNMTVLLKLLEAHRRTMENVNRQQRPPSPKGIRRRTVGQPSSGLPQQRVLGPSPQSRNVATPIAQRANTQRGSTGNTMSRTSGGSNRSPYGDSSNVKQEPTSPFEQLRKGYNNNKRPAQTSLLSPPSKKPSLEEVKEFAEQQRMRKQIAAEYGDNDPEYDGGMLYDDVHAGDDDDDDMPPQPSTSKQQSPQGTQTQLEHGSTTIILKQDSPSQTPTIIVKDSSNAKLNHTKIIAEVLRQYPHIVKGHKNIKLKIMPNTPAAPTEKSAPATVKPPANQSSATTSPHKKLHVSFKADKSTPLITAQQKAASSQQKSGTSQTTGNQGTGANPPANTAAAQKRRIDSKTMHALIAQGAENTTGPWLCLRCGVNGRPISIPSYRGFRRHLINTHKETIDPALCEHCGWRSVNNRELHFHMYMEHQTKSLLYTFAECALCNQSYRTKGELEAHINEVHTDDNKQQCIYCNKVFEQELQLYRHMKSYHKEQALEDGIIDETDEEFLGSQDEEEEAEGDEEQEPEQTGKVRILSDISLPATSAITVQQAQQEQLQEEDVEQVQQEVKFVGADGNEVELTDEQRKEILSQLNQQQAGATAGGVVMVLSEPEAEHVKQETDEKSLAGTEEEYDDSQIYSELGAADSVESAKKNIADESKESIDNLEWAENLIAESEEQSNKEPKSDKPRDDISEKLKELTGDWTEDENDDDVDDKPATAELASELANKDPEPTVHEEEDDIDLALQSLHKGPEEATEEKASEESVTSADDAVDAVPNINSQPEKMDVDSEAADEKASKAEVQIKKEAELENDQEEFIKEDSPIPHSDSVAELREAVTASEGEDDVHLEADNIRKELLDELIAEAEKPDQEKDIVQSEENATTEALDRSVTDEDDLVPPTQVSTEQMEIDEPAAEKAAENNEDTRTADEKEAVEDKPNQTQDVTTAEKPTLESAKAGDEATSGEAASVDKVKSLISEWGDDDEDEDENGVSAAAKEEL

Target ORF information:

RefSeq Version	NM_169632.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster centrosomal protein 190kD, transcript variant B (Cp190), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169632.2

ORF Insert Sequence:

ATGGGTGAAG TCAAGTCCGT GAAAGTGGAC AACTGGGGAG TCTTCTTCCT GCAGAAGCTG 
CAGAACTTCT TTAATAAAAC GGATTACTGC GATTTGACGC TGCAGTTTCG GGACAATTCA 
CAGCTAAAGG TACACCGTCT GGTGCTCAGC GCCTGCACCG ACTACTTCAA TGTACTGGAG 
CAGACCTGCG AGATCGTCGA CGATGCCCTC ATCATGCCCA ACGAGTTCCA GGCGGACGTG 
GTGGTGCCCA TTGTCAACTT CATGTACACA GGCACCCTGG AGTTTGAGCT TAAAATGTAC 
GGCAAGCTGT TGCGCACCGC CAAGGAAATG AACATGACGG TGCTGTTGAA GCTGCTAGAG 
GCGCACCGTC GCACCATGGA GAACGTTAAT CGCCAGCAAA GGCCGCCTAG TCCAAAGGGA 
ATACGCCGGC GCACAGTTGG ACAGCCCAGT TCGGGTCTTC CACAACAGCG CGTCTTGGGT 
CCTTCGCCGC AATCGCGAAA CGTAGCCACA CCCATCGCGC AACGAGCCAA CACACAGCGC 
GGGTCAACTG GCAACACAAT GAGCCGAACA TCAGGTGGCT CAAATCGTTC GCCGTATGGA 
GATAGTAGCA ATGTGAAGCA GGAGCCAACG TCACCATTCG AGCAGCTGCG AAAGGGTTAT 
AACAATAACA AGAGACCAGC ACAGACAAGC CTGCTATCGC CGCCATCCAA GAAGCCCAGT 
TTGGAAGAGG TTAAGGAATT CGCTGAGCAA CAACGGATGC GAAAGCAAAT CGCCGCTGAG 
TACGGAGATA ACGATCCTGA GTACGATGGA GGAATGCTTT ATGATGATGT CCATGCCGGG 
GACGATGATG ACGATGATAT GCCACCGCAG CCATCTACAT CTAAGCAGCA ATCCCCACAG 
GGTACACAGA CCCAACTAGA ACATGGATCC ACCACCATTA TCTTGAAACA GGACTCACCC 
AGCCAGACCC CAACAATAAT TGTCAAGGAC TCCTCCAACG CCAAGCTGAA TCACACCAAG 
ATCATTGCCG AAGTCTTGCG CCAGTATCCG CACATTGTAA AGGGCCACAA AAACATAAAG 
CTAAAGATTA TGCCCAATAC CCCGGCAGCG CCGACCGAAA AATCTGCTCC AGCGACAGTA 
AAACCCCCGG CGAACCAGTC ATCCGCAACT ACATCGCCAC ACAAGAAACT GCACGTTTCT 
TTCAAAGCCG ACAAGAGTAC GCCACTAATC ACAGCACAAC AGAAGGCGGC TAGCTCTCAG 
CAAAAGTCGG GAACCTCGCA GACAACCGGT AACCAGGGAA CCGGGGCGAA TCCACCAGCA 
AATACAGCCG CAGCCCAGAA GAGACGCATT GATAGTAAGA CAATGCACGC CTTGATCGCT 
CAGGGAGCAG AGAATACTAC TGGGCCCTGG CTGTGCCTGA GATGCGGCGT AAACGGACGA 
CCCATTAGCA TTCCGTCGTA CCGAGGCTTC CGACGCCATC TGATCAACAC GCACAAGGAG 
ACAATTGATC CGGCGCTTTG TGAGCATTGC GGGTGGAGGT CTGTCAACAA CCGGGAACTG 
CATTTCCACA TGTATATGGA GCACCAGACC AAGTCGCTGC TGTATACGTT TGCCGAGTGC 
GCGCTATGCA ACCAGTCTTA CCGGACCAAG GGTGAGTTGG AGGCGCACAT AAACGAGGTG 
CACACGGACG ACAACAAGCA GCAGTGCATC TACTGCAACA AGGTGTTCGA GCAAGAGCTG 
CAGCTGTACA GACACATGAA GAGCTACCAC AAGGAGCAGG CTTTGGAGGA TGGCATTATC 
GATGAAACGG ACGAAGAATT CCTCGGCTCG CAAGACGAGG AGGAAGAGGC CGAAGGAGAC 
GAAGAGCAGG AACCAGAGCA GACTGGCAAA GTGCGAATCC TTTCGGACAT AAGTCTACCA 
GCCACCAGTG CCATCACAGT GCAGCAAGCT CAACAGGAGC AACTGCAAGA GGAAGATGTT 
GAACAGGTGC AGCAGGAAGT CAAATTCGTG GGCGCCGATG GCAATGAGGT GGAGCTTACT 
GATGAGCAGC GCAAAGAGAT TCTCTCACAG CTTAACCAGC AGCAAGCAGG CGCCACTGCC 
GGCGGTGTGG TAATGGTGCT CAGTGAGCCA GAAGCGGAGC ACGTCAAGCA GGAGACCGAT 
GAAAAATCCC TGGCCGGCAC CGAAGAGGAA TATGATGACA GTCAAATCTA TAGCGAACTG 
GGTGCTGCCG ACTCTGTGGA GAGCGCCAAA AAGAACATTG CTGATGAAAG CAAAGAGTCC 
ATTGACAACC TGGAATGGGC CGAAAACCTT ATAGCCGAGT CTGAAGAACA GAGCAACAAG 
GAGCCAAAAT CGGATAAGCC GCGAGATGAC ATTAGTGAAA AGCTAAAGGA GCTTACCGGC 
GACTGGACGG AGGATGAGAA CGATGACGAT GTCGACGATA AGCCCGCTAC CGCAGAGCTT 
GCATCCGAAC TAGCCAACAA GGACCCTGAG CCAACAGTAC ACGAGGAAGA AGATGATATT 
GACTTGGCTC TCCAGTCTCT ACACAAGGGA CCCGAAGAGG CAACTGAAGA AAAGGCAAGT 
GAGGAGTCTG TCACCAGTGC GGACGATGCA GTTGACGCAG TCCCCAATAT AAATAGTCAG 
CCCGAGAAAA TGGATGTGGA CTCAGAAGCG GCGGACGAGA AGGCTTCGAA GGCCGAGGTC 
CAGATCAAGA AAGAGGCTGA GCTTGAAAAC GATCAAGAGG AATTTATCAA AGAGGATTCA 
CCAATTCCGC ATTCCGATTC CGTGGCGGAA TTAAGAGAGG CGGTAACTGC CTCTGAGGGC 
GAGGATGATG TGCATCTAGA GGCCGATAAC ATACGCAAAG AGCTGCTAGA TGAGTTAATT 
GCCGAGGCCG AGAAACCTGA TCAGGAGAAG GACATTGTTC AATCTGAAGA GAATGCGACG 
ACAGAGGCGT TAGATAGGTC AGTAACGGAC GAGGATGACC TGGTGCCACC TACGCAGGTA 
TCTACCGAAC AAATGGAGAT TGATGAACCA GCAGCGGAAA AAGCAGCCGA AAATAATGAA 
GATACTAGAA CGGCAGACGA GAAGGAAGCC GTCGAGGACA AACCAAACCA GACACAGGAT 
GTAACCACTG CAGAAAAGCC GACACTAGAA AGTGCAAAAG CAGGTGATGA AGCAACGTCA 
GGTGAAGCAG CCAGCGTGGA CAAGGTCAAG AGCCTCATTA GCGAATGGGG TGACGACGAT 
GAGGACGAGG ATGAGAATGG GGTTAGTGCG GCGGCGAAGG AGGAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cp190 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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