eIF2gamma cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eIF2gamma
Entrez Gene ID	41843
Full Name	Eukaryotic initiation factor 2gamma
Synonyms	51kDa protein,CG43665,CG6476,Dmel\CG43665,eIF-2G,eIF2G,eIF2gamma
General protein information	Preferred Names Eukaryotic initiation factor 2gamma Names CG43665 gene product from transcript CG43665-RD CG43665-PB CG43665-PC CG43665-PD eIF-2gamma-PB eIF-2gamma-PC eIF-2gamma-PD eukaryotic initiation factor 2gamma gamma subunit of the translation initiation factor eIF2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88E6-88E8

mRNA and Protein(s)

mRNA	Protein	Name
NM_169629.2	NP_731993.1	eukaryotic translation initiation factor 2 subunit gamma, isoform B
NM_169630.2	NP_731994.1	eukaryotic translation initiation factor 2 subunit gamma, isoform C
NM_001275658.1	NP_001262587.1	eukaryotic translation initiation factor 2 subunit gamma, isoform D

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EIF2S3	XP_003952163.1
Danio rerio (zebrafish)	eif2s3	NP_997876.1
Rattus norvegicus (Norway rat)	Eif2s3x	NP_001094012.1
Gallus gallus (chicken)	EIF2S3	NP_001006260.1
Caenorhabditis elegans (roundworm)	CELE_Y39G10AR.8	NP_490960.1
Arabidopsis thaliana (thale cress)	EIF2 GAMMA	NP_171913.1
Xenopus tropicalis (tropical clawed frog)	eif2s3	NP_001011456.1
Homo sapiens (human)	EIF2S3	NP_001406.1
Canis lupus familiaris (dog)	EIF2S3	XP_537983.2
Drosophila melanogaster (fruit fly)	eIF-2gamma	NP_731993.1
Oryza sativa (rice)	Os12g0176800	NP_001066299.1
Bos taurus (cattle)	EIF2S3	NP_001039582.1
Mus musculus (house mouse)	Eif2s3x	NP_036140.1
	EIF2S3	XP_001089902.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003597	XP_003436534.1
Oryza sativa (rice)	Os12g0607100	NP_001067233.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A balance between euchromatic (JIL-1) and heterochromatic [SU(var)2-5 and SU(var)3-9] factors regulates position-effect variegation in Drosophila.
Title: A balance between euchromatic (JIL-1) and heterochromatic [SU(var)2-5 and SU(var)3-9] factors regulates position-effect variegation in Drosophila.

the SUUR protein is required for the SU(VAR)3-9 enzyme to bind to the salivary gland polytene chromosomes
Title: The SUUR protein is involved in binding of SU(VAR)3-9 and methylation of H3K9 and H3K27 in chromosomes of Drosophila melanogaster.

Data show that animals with reduced heterochromatin, such as Stat92E transheterozygotes, Su(var)205 heterozygotes, or Su(var)3-9 transheterozygotes, were less viable than wild-type controls.
Title: Unphosphorylated STAT and heterochromatin protect genome stability.

RNA helicase interacts with the well-characterized histone methyltransferase SU(VAR)3-9 via its N-terminus, which provides a potential mechanism for the targeting of H3K9 methylation to highly regulated genes.
Title: The RNA helicase Rm62 cooperates with SU(VAR)3-9 to re-silence active transcription in Drosophila melanogaster.

The genetic interactions between dSETDB1 and Su(var)3-9 mutations emphasize the importance of maintaining the activities of these histone methyltransferases in balance for normal genome function.
Title: Multiple SET methyltransferases are required to maintain normal heterochromatin domains in the genome of Drosophila melanogaster.

The following eIF2gamma gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIF2gamma cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04952	NM_001275658.1 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 2 subunit gamma (eIF2gamma), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04952	NM_169629.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 2 subunit gamma (eIF2gamma), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04953	NM_169630.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 2 subunit gamma (eIF2gamma), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eIF2gamma ( NM_169629.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731993.1 Drosophila melanogaster eukaryotic translation initiation factor 2 subunit gamma (eIF2gamma), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04952
Clone ID Related Accession (Same CDS sequence)	NM_169629.2 , NM_001275658.1
Accession Version	NM_169629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 2 subunit gamma, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169629.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGG CTGAAGCCCA GATCGGTGTG AACAGGAACT TGCAGAAGCA GGACCTCAGC 
AACTTGGACG TCTCTAAACT AACGCCTCTA TCACCGGAGG TCATCTCCCG ACAGGCGACC 
ATCAACATCG GCACCATCGG TCATGTGGCT CACGGCAAAT CCACGGTGGT CAAAGCCATA 
TCCGGCGTGC AGACTGTGCG CTTCAAGAAC GAACTGGAGC GCAACATCAC AATCAAGCTG 
GGTTATGCCA ACGCCAAAAT CTACAAGTGT GACAACCCCA AGTGCCCGCG CCCGGCCAGC 
TTCGTATCGG ATGCATCCAG CAAGGACGAC AGCTTGCCCT GCACTCGGCT CAACTGCTCC 
GGCAACTTCA GACTGGTGCG ACATGTGAGC TTCGTGGATT GCCCCGGTCA CGACATTCTT 
ATGGCTACCA TGTTGAACGG TGCTGCTGTG ATGGACGCTG CTCTGCTGCT CATTGCTGGC 
AATGAATCCT GTCCACAACC GCAAACCTCT GAGCATTTGG CCGCCATTGA GATTATGAAG 
CTGAAACAGA TTCTGATCTT GCAAAACAAG ATCGATCTGA TCAAGGAGAG CCAGGCGAAA 
GAGCAGTACG AGGAGATCAC CAAATTCGTC CAGGGCACCG TAGCTGAGGG CGCTCCGATC 
ATTCCGATCT CGGCCCAGCT TAAGTACAAC ATCGATGTGC TCTGCGAGTA CATTGTGAAC 
AAGATACCAG TGCCGCCGCG TGACTTCAAT GCCCCGCCCC GTCTGATCGT CATCCGATCC 
TTCGACGTGA ACAAGCCAGG CTGTGAGGTG GCAGACCTTA AGGGCGGTGT GGCCGGTGGC 
TCCATTCTGA GCGGCGTGCT GAAGGTAGGC CAAGAGATCG AGGTGCGTCC GGGCGTTGTG 
ACCAAGGATA GCGATGGCAA CATCACCTGC CGTCCAATCT TCTCCCGTAT CGTCTCGCTC 
TTCGCCGAAC AGAATGAGCT GCAGTACGCC GTGCCCGGCG GTCTGATTGG TGTGGGCACC 
AAGATCGATC CTACGCTGTG CCGTGCCGAT CGTTTGGTCG GTCAGGTGCT AGGCGCCGTT 
GGCCAGCTGC CGGACATCTA CCAGGAGCTG GAAATCTCGT ATTACCTGCT GCGTCGCCTT 
CTCGGCGTCC GTACCGATGG TGACAAGAAG GGAGCGCGCG TTGAGAAGCT GCAGAAGAAC 
GAAATCCTGT TGGTTAATAT CGGATCCCTC AGCACTGGTG GCCGCATCTC GGCCACCAAA 
GGTGATTTGG CCAAGATCGT TCTTACGACG CCTGTGTGCA CGGAGAAGGG CGAGAAGATT 
GCACTCAGCA GACGTGTGGA GAACCATTGG CGTTTGATTG GCTGGGGTCA GATCTTCGGA 
GGCAAGACAA TCACACCCGT TCTGGACAGC CAGGTGGCAA AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731993.1
CDS	112..1539
Translation	MATAEAQIGVNRNLQKQDLSNLDVSKLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRFKNELERNITIKLGYANAKIYKCDNPKCPRPASFVSDASSKDDSLPCTRLNCSGNFRLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKQILILQNKIDLIKESQAKEQYEEITKFVQGTVAEGAPIIPISAQLKYNIDVLCEYIVNKIPVPPRDFNAPPRLIVIRSFDVNKPGCEVADLKGGVAGGSILSGVLKVGQEIEVRPGVVTKDSDGNITCRPIFSRIVSLFAEQNELQYAVPGGLIGVGTKIDPTLCRADRLVGQVLGAVGQLPDIYQELEISYYLLRRLLGVRTDGDKKGARVEKLQKNEILLVNIGSLSTGGRISATKGDLAKIVLTTPVCTEKGEKIALSRRVENHWRLIGWGQIFGGKTITPVLDSQVAKK

Target ORF information:

RefSeq Version	NM_169629.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 2 subunit gamma (eIF2gamma), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169629.2

ORF Insert Sequence:

ATGGCCACGG CTGAAGCCCA GATCGGTGTG AACAGGAACT TGCAGAAGCA GGACCTCAGC 
AACTTGGACG TCTCTAAACT AACGCCTCTA TCACCGGAGG TCATCTCCCG ACAGGCGACC 
ATCAACATCG GCACCATCGG TCATGTGGCT CACGGCAAAT CCACGGTGGT CAAAGCCATA 
TCCGGCGTGC AGACTGTGCG CTTCAAGAAC GAACTGGAGC GCAACATCAC AATCAAGCTG 
GGTTATGCCA ACGCCAAAAT CTACAAGTGT GACAACCCCA AGTGCCCGCG CCCGGCCAGC 
TTCGTATCGG ATGCATCCAG CAAGGACGAC AGCTTGCCCT GCACTCGGCT CAACTGCTCC 
GGCAACTTCA GACTGGTGCG ACATGTGAGC TTCGTGGATT GCCCCGGTCA CGACATTCTT 
ATGGCTACCA TGTTGAACGG TGCTGCTGTG ATGGACGCTG CTCTGCTGCT CATTGCTGGC 
AATGAATCCT GTCCACAACC GCAAACCTCT GAGCATTTGG CCGCCATTGA GATTATGAAG 
CTGAAACAGA TTCTGATCTT GCAAAACAAG ATCGATCTGA TCAAGGAGAG CCAGGCGAAA 
GAGCAGTACG AGGAGATCAC CAAATTCGTC CAGGGCACCG TAGCTGAGGG CGCTCCGATC 
ATTCCGATCT CGGCCCAGCT TAAGTACAAC ATCGATGTGC TCTGCGAGTA CATTGTGAAC 
AAGATACCAG TGCCGCCGCG TGACTTCAAT GCCCCGCCCC GTCTGATCGT CATCCGATCC 
TTCGACGTGA ACAAGCCAGG CTGTGAGGTG GCAGACCTTA AGGGCGGTGT GGCCGGTGGC 
TCCATTCTGA GCGGCGTGCT GAAGGTAGGC CAAGAGATCG AGGTGCGTCC GGGCGTTGTG 
ACCAAGGATA GCGATGGCAA CATCACCTGC CGTCCAATCT TCTCCCGTAT CGTCTCGCTC 
TTCGCCGAAC AGAATGAGCT GCAGTACGCC GTGCCCGGCG GTCTGATTGG TGTGGGCACC 
AAGATCGATC CTACGCTGTG CCGTGCCGAT CGTTTGGTCG GTCAGGTGCT AGGCGCCGTT 
GGCCAGCTGC CGGACATCTA CCAGGAGCTG GAAATCTCGT ATTACCTGCT GCGTCGCCTT 
CTCGGCGTCC GTACCGATGG TGACAAGAAG GGAGCGCGCG TTGAGAAGCT GCAGAAGAAC 
GAAATCCTGT TGGTTAATAT CGGATCCCTC AGCACTGGTG GCCGCATCTC GGCCACCAAA 
GGTGATTTGG CCAAGATCGT TCTTACGACG CCTGTGTGCA CGGAGAAGGG CGAGAAGATT 
GCACTCAGCA GACGTGTGGA GAACCATTGG CGTTTGATTG GCTGGGGTCA GATCTTCGGA 
GGCAAGACAA TCACACCCGT TCTGGACAGC CAGGTGGCAA AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF2gamma ( NM_001275658.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262587.1 Drosophila melanogaster eukaryotic translation initiation factor 2 subunit gamma (eIF2gamma), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa04952
Clone ID Related Accession (Same CDS sequence)	NM_169629.2 , NM_001275658.1
Accession Version	NM_001275658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 2 subunit gamma, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGG CTGAAGCCCA GATCGGTGTG AACAGGAACT TGCAGAAGCA GGACCTCAGC 
AACTTGGACG TCTCTAAACT AACGCCTCTA TCACCGGAGG TCATCTCCCG ACAGGCGACC 
ATCAACATCG GCACCATCGG TCATGTGGCT CACGGCAAAT CCACGGTGGT CAAAGCCATA 
TCCGGCGTGC AGACTGTGCG CTTCAAGAAC GAACTGGAGC GCAACATCAC AATCAAGCTG 
GGTTATGCCA ACGCCAAAAT CTACAAGTGT GACAACCCCA AGTGCCCGCG CCCGGCCAGC 
TTCGTATCGG ATGCATCCAG CAAGGACGAC AGCTTGCCCT GCACTCGGCT CAACTGCTCC 
GGCAACTTCA GACTGGTGCG ACATGTGAGC TTCGTGGATT GCCCCGGTCA CGACATTCTT 
ATGGCTACCA TGTTGAACGG TGCTGCTGTG ATGGACGCTG CTCTGCTGCT CATTGCTGGC 
AATGAATCCT GTCCACAACC GCAAACCTCT GAGCATTTGG CCGCCATTGA GATTATGAAG 
CTGAAACAGA TTCTGATCTT GCAAAACAAG ATCGATCTGA TCAAGGAGAG CCAGGCGAAA 
GAGCAGTACG AGGAGATCAC CAAATTCGTC CAGGGCACCG TAGCTGAGGG CGCTCCGATC 
ATTCCGATCT CGGCCCAGCT TAAGTACAAC ATCGATGTGC TCTGCGAGTA CATTGTGAAC 
AAGATACCAG TGCCGCCGCG TGACTTCAAT GCCCCGCCCC GTCTGATCGT CATCCGATCC 
TTCGACGTGA ACAAGCCAGG CTGTGAGGTG GCAGACCTTA AGGGCGGTGT GGCCGGTGGC 
TCCATTCTGA GCGGCGTGCT GAAGGTAGGC CAAGAGATCG AGGTGCGTCC GGGCGTTGTG 
ACCAAGGATA GCGATGGCAA CATCACCTGC CGTCCAATCT TCTCCCGTAT CGTCTCGCTC 
TTCGCCGAAC AGAATGAGCT GCAGTACGCC GTGCCCGGCG GTCTGATTGG TGTGGGCACC 
AAGATCGATC CTACGCTGTG CCGTGCCGAT CGTTTGGTCG GTCAGGTGCT AGGCGCCGTT 
GGCCAGCTGC CGGACATCTA CCAGGAGCTG GAAATCTCGT ATTACCTGCT GCGTCGCCTT 
CTCGGCGTCC GTACCGATGG TGACAAGAAG GGAGCGCGCG TTGAGAAGCT GCAGAAGAAC 
GAAATCCTGT TGGTTAATAT CGGATCCCTC AGCACTGGTG GCCGCATCTC GGCCACCAAA 
GGTGATTTGG CCAAGATCGT TCTTACGACG CCTGTGTGCA CGGAGAAGGG CGAGAAGATT 
GCACTCAGCA GACGTGTGGA GAACCATTGG CGTTTGATTG GCTGGGGTCA GATCTTCGGA 
GGCAAGACAA TCACACCCGT TCTGGACAGC CAGGTGGCAA AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262587.1
CDS	112..1539
Translation	MATAEAQIGVNRNLQKQDLSNLDVSKLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRFKNELERNITIKLGYANAKIYKCDNPKCPRPASFVSDASSKDDSLPCTRLNCSGNFRLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKQILILQNKIDLIKESQAKEQYEEITKFVQGTVAEGAPIIPISAQLKYNIDVLCEYIVNKIPVPPRDFNAPPRLIVIRSFDVNKPGCEVADLKGGVAGGSILSGVLKVGQEIEVRPGVVTKDSDGNITCRPIFSRIVSLFAEQNELQYAVPGGLIGVGTKIDPTLCRADRLVGQVLGAVGQLPDIYQELEISYYLLRRLLGVRTDGDKKGARVEKLQKNEILLVNIGSLSTGGRISATKGDLAKIVLTTPVCTEKGEKIALSRRVENHWRLIGWGQIFGGKTITPVLDSQVAKK

Target ORF information:

RefSeq Version	NM_001275658.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 2 subunit gamma (eIF2gamma), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275658.1

ORF Insert Sequence:

ATGGCCACGG CTGAAGCCCA GATCGGTGTG AACAGGAACT TGCAGAAGCA GGACCTCAGC 
AACTTGGACG TCTCTAAACT AACGCCTCTA TCACCGGAGG TCATCTCCCG ACAGGCGACC 
ATCAACATCG GCACCATCGG TCATGTGGCT CACGGCAAAT CCACGGTGGT CAAAGCCATA 
TCCGGCGTGC AGACTGTGCG CTTCAAGAAC GAACTGGAGC GCAACATCAC AATCAAGCTG 
GGTTATGCCA ACGCCAAAAT CTACAAGTGT GACAACCCCA AGTGCCCGCG CCCGGCCAGC 
TTCGTATCGG ATGCATCCAG CAAGGACGAC AGCTTGCCCT GCACTCGGCT CAACTGCTCC 
GGCAACTTCA GACTGGTGCG ACATGTGAGC TTCGTGGATT GCCCCGGTCA CGACATTCTT 
ATGGCTACCA TGTTGAACGG TGCTGCTGTG ATGGACGCTG CTCTGCTGCT CATTGCTGGC 
AATGAATCCT GTCCACAACC GCAAACCTCT GAGCATTTGG CCGCCATTGA GATTATGAAG 
CTGAAACAGA TTCTGATCTT GCAAAACAAG ATCGATCTGA TCAAGGAGAG CCAGGCGAAA 
GAGCAGTACG AGGAGATCAC CAAATTCGTC CAGGGCACCG TAGCTGAGGG CGCTCCGATC 
ATTCCGATCT CGGCCCAGCT TAAGTACAAC ATCGATGTGC TCTGCGAGTA CATTGTGAAC 
AAGATACCAG TGCCGCCGCG TGACTTCAAT GCCCCGCCCC GTCTGATCGT CATCCGATCC 
TTCGACGTGA ACAAGCCAGG CTGTGAGGTG GCAGACCTTA AGGGCGGTGT GGCCGGTGGC 
TCCATTCTGA GCGGCGTGCT GAAGGTAGGC CAAGAGATCG AGGTGCGTCC GGGCGTTGTG 
ACCAAGGATA GCGATGGCAA CATCACCTGC CGTCCAATCT TCTCCCGTAT CGTCTCGCTC 
TTCGCCGAAC AGAATGAGCT GCAGTACGCC GTGCCCGGCG GTCTGATTGG TGTGGGCACC 
AAGATCGATC CTACGCTGTG CCGTGCCGAT CGTTTGGTCG GTCAGGTGCT AGGCGCCGTT 
GGCCAGCTGC CGGACATCTA CCAGGAGCTG GAAATCTCGT ATTACCTGCT GCGTCGCCTT 
CTCGGCGTCC GTACCGATGG TGACAAGAAG GGAGCGCGCG TTGAGAAGCT GCAGAAGAAC 
GAAATCCTGT TGGTTAATAT CGGATCCCTC AGCACTGGTG GCCGCATCTC GGCCACCAAA 
GGTGATTTGG CCAAGATCGT TCTTACGACG CCTGTGTGCA CGGAGAAGGG CGAGAAGATT 
GCACTCAGCA GACGTGTGGA GAACCATTGG CGTTTGATTG GCTGGGGTCA GATCTTCGGA 
GGCAAGACAA TCACACCCGT TCTGGACAGC CAGGTGGCAA AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF2gamma ( NM_169630.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731994.1 Drosophila melanogaster eukaryotic translation initiation factor 2 subunit gamma (eIF2gamma), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04953
Clone ID Related Accession (Same CDS sequence)	NM_169630.2
Accession Version	NM_169630.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 2 subunit gamma, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169630.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCTGA GAGGGGATGT TTTGTTAGGG GGTGTGGCGG CGGACGTCTC TAAACTAACG 
CCTCTATCAC CGGAGGTCAT CTCCCGACAG GCGACCATCA ACATCGGCAC CATCGGTCAT 
GTGGCTCACG GCAAATCCAC GGTGGTCAAA GCCATATCCG GCGTGCAGAC TGTGCGCTTC 
AAGAACGAAC TGGAGCGCAA CATCACAATC AAGCTGGGTT ATGCCAACGC CAAAATCTAC 
AAGTGTGACA ACCCCAAGTG CCCGCGCCCG GCCAGCTTCG TATCGGATGC ATCCAGCAAG 
GACGACAGCT TGCCCTGCAC TCGGCTCAAC TGCTCCGGCA ACTTCAGACT GGTGCGACAT 
GTGAGCTTCG TGGATTGCCC CGGTCACGAC ATTCTTATGG CTACCATGTT GAACGGTGCT 
GCTGTGATGG ACGCTGCTCT GCTGCTCATT GCTGGCAATG AATCCTGTCC ACAACCGCAA 
ACCTCTGAGC ATTTGGCCGC CATTGAGATT ATGAAGCTGA AACAGATTCT GATCTTGCAA 
AACAAGATCG ATCTGATCAA GGAGAGCCAG GCGAAAGAGC AGTACGAGGA GATCACCAAA 
TTCGTCCAGG GCACCGTAGC TGAGGGCGCT CCGATCATTC CGATCTCGGC CCAGCTTAAG 
TACAACATCG ATGTGCTCTG CGAGTACATT GTGAACAAGA TACCAGTGCC GCCGCGTGAC 
TTCAATGCCC CGCCCCGTCT GATCGTCATC CGATCCTTCG ACGTGAACAA GCCAGGCTGT 
GAGGTGGCAG ACCTTAAGGG CGGTGTGGCC GGTGGCTCCA TTCTGAGCGG CGTGCTGAAG 
GTAGGCCAAG AGATCGAGGT GCGTCCGGGC GTTGTGACCA AGGATAGCGA TGGCAACATC 
ACCTGCCGTC CAATCTTCTC CCGTATCGTC TCGCTCTTCG CCGAACAGAA TGAGCTGCAG 
TACGCCGTGC CCGGCGGTCT GATTGGTGTG GGCACCAAGA TCGATCCTAC GCTGTGCCGT 
GCCGATCGTT TGGTCGGTCA GGTGCTAGGC GCCGTTGGCC AGCTGCCGGA CATCTACCAG 
GAGCTGGAAA TCTCGTATTA CCTGCTGCGT CGCCTTCTCG GCGTCCGTAC CGATGGTGAC 
AAGAAGGGAG CGCGCGTTGA GAAGCTGCAG AAGAACGAAA TCCTGTTGGT TAATATCGGA 
TCCCTCAGCA CTGGTGGCCG CATCTCGGCC ACCAAAGGTG ATTTGGCCAA GATCGTTCTT 
ACGACGCCTG TGTGCACGGA GAAGGGCGAG AAGATTGCAC TCAGCAGACG TGTGGAGAAC 
CATTGGCGTT TGATTGGCTG GGGTCAGATC TTCGGAGGCA AGACAATCAC ACCCGTTCTG 
GACAGCCAGG TGGCAAAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731994.1
CDS	100..1503
Translation	MHLRGDVLLGGVAADVSKLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRFKNELERNITIKLGYANAKIYKCDNPKCPRPASFVSDASSKDDSLPCTRLNCSGNFRLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKQILILQNKIDLIKESQAKEQYEEITKFVQGTVAEGAPIIPISAQLKYNIDVLCEYIVNKIPVPPRDFNAPPRLIVIRSFDVNKPGCEVADLKGGVAGGSILSGVLKVGQEIEVRPGVVTKDSDGNITCRPIFSRIVSLFAEQNELQYAVPGGLIGVGTKIDPTLCRADRLVGQVLGAVGQLPDIYQELEISYYLLRRLLGVRTDGDKKGARVEKLQKNEILLVNIGSLSTGGRISATKGDLAKIVLTTPVCTEKGEKIALSRRVENHWRLIGWGQIFGGKTITPVLDSQVAKK

Target ORF information:

RefSeq Version	NM_169630.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 2 subunit gamma (eIF2gamma), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169630.2

ORF Insert Sequence:

ATGCATCTGA GAGGGGATGT TTTGTTAGGG GGTGTGGCGG CGGACGTCTC TAAACTAACG 
CCTCTATCAC CGGAGGTCAT CTCCCGACAG GCGACCATCA ACATCGGCAC CATCGGTCAT 
GTGGCTCACG GCAAATCCAC GGTGGTCAAA GCCATATCCG GCGTGCAGAC TGTGCGCTTC 
AAGAACGAAC TGGAGCGCAA CATCACAATC AAGCTGGGTT ATGCCAACGC CAAAATCTAC 
AAGTGTGACA ACCCCAAGTG CCCGCGCCCG GCCAGCTTCG TATCGGATGC ATCCAGCAAG 
GACGACAGCT TGCCCTGCAC TCGGCTCAAC TGCTCCGGCA ACTTCAGACT GGTGCGACAT 
GTGAGCTTCG TGGATTGCCC CGGTCACGAC ATTCTTATGG CTACCATGTT GAACGGTGCT 
GCTGTGATGG ACGCTGCTCT GCTGCTCATT GCTGGCAATG AATCCTGTCC ACAACCGCAA 
ACCTCTGAGC ATTTGGCCGC CATTGAGATT ATGAAGCTGA AACAGATTCT GATCTTGCAA 
AACAAGATCG ATCTGATCAA GGAGAGCCAG GCGAAAGAGC AGTACGAGGA GATCACCAAA 
TTCGTCCAGG GCACCGTAGC TGAGGGCGCT CCGATCATTC CGATCTCGGC CCAGCTTAAG 
TACAACATCG ATGTGCTCTG CGAGTACATT GTGAACAAGA TACCAGTGCC GCCGCGTGAC 
TTCAATGCCC CGCCCCGTCT GATCGTCATC CGATCCTTCG ACGTGAACAA GCCAGGCTGT 
GAGGTGGCAG ACCTTAAGGG CGGTGTGGCC GGTGGCTCCA TTCTGAGCGG CGTGCTGAAG 
GTAGGCCAAG AGATCGAGGT GCGTCCGGGC GTTGTGACCA AGGATAGCGA TGGCAACATC 
ACCTGCCGTC CAATCTTCTC CCGTATCGTC TCGCTCTTCG CCGAACAGAA TGAGCTGCAG 
TACGCCGTGC CCGGCGGTCT GATTGGTGTG GGCACCAAGA TCGATCCTAC GCTGTGCCGT 
GCCGATCGTT TGGTCGGTCA GGTGCTAGGC GCCGTTGGCC AGCTGCCGGA CATCTACCAG 
GAGCTGGAAA TCTCGTATTA CCTGCTGCGT CGCCTTCTCG GCGTCCGTAC CGATGGTGAC 
AAGAAGGGAG CGCGCGTTGA GAAGCTGCAG AAGAACGAAA TCCTGTTGGT TAATATCGGA 
TCCCTCAGCA CTGGTGGCCG CATCTCGGCC ACCAAAGGTG ATTTGGCCAA GATCGTTCTT 
ACGACGCCTG TGTGCACGGA GAAGGGCGAG AAGATTGCAC TCAGCAGACG TGTGGAGAAC 
CATTGGCGTT TGATTGGCTG GGGTCAGATC TTCGGAGGCA AGACAATCAC ACCCGTTCTG 
GACAGCCAGG TGGCAAAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eIF2gamma cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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