Caf1-55 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Caf1-55
Entrez Gene ID	41836
Full Name	Chromatin assembly factor 1, p55 subunit
Synonyms	154659_at,55,CAF-1,CAF1,CAF1-55,CAF1-p55,CAF1p55,CG4236,Caf-1,Caf1,Caf1 (p55),Caf1/p55,Caf1p55,Dmel\CG4236,MSI1/RbAp48/CAC3/LIN-53,NURF,NURF 55,NURF-55,NURF55,Nurf,Nurf 55,Nurf-55,Nurf55,P55,RBAP48,RbAp46/48,RbAp48,S(ls)3,caf-1,caf1,caf1 p55,d-CAF1,dCAF-1,dCAF-1 p55,dCAF-1-p55,dCAF1,dCAF1-p55,dNURF,p55,p55 CAF1,p55/NURF-55,p55CAF1
General protein information	Preferred Names Chromatin assembly factor 1, p55 subunit Names CG4236 gene product from transcript CG4236-RA CG4236-PA CG4236-PB Caf1-55-PA Caf1-55-PB Nurf55/CAF1 chromatin assembly Factor-1 chromatin assembly factor 1 chromatin assembly factor 1 subunit chromatin assembly factor 1, p55 subunit chromatin associated factor-1 subunit nucleosome remodeling factor nucleosome remodeling factor - 55kD p55
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88E3-88E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_079630.4	NP_524354.1	chromatin assembly factor 1, p55 subunit, isoform A
NM_001170146.2	NP_001163617.1	chromatin assembly factor 1, p55 subunit, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rbbp4	NP_033056.2
Gallus gallus (chicken)	RBBP4	NP_990183.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003228	XP_312936.3
Homo sapiens (human)	RBBP4	NP_005601.1
Bos taurus (cattle)	RBBP4	NP_001070481.1
Danio rerio (zebrafish)	rbb4	NP_997760.1
Schizosaccharomyces pombe (fission yeast)	mis16	NP_587881.1
Neurospora crassa	NCU06679	XP_960994.2
Arabidopsis thaliana (thale cress)	MSI1	NP_200631.1
Pan troglodytes (chimpanzee)	RBBP4	NP_001233303.1
Drosophila melanogaster (fruit fly)	Caf1	NP_001163617.1
Magnaporthe oryzae (rice blast fungus)	MGG_07323	XP_003715557.1
Oryza sativa (rice)	Os03g0640100	NP_001050741.1
Canis lupus familiaris (dog)	RBBP4	XP_864445.1
Rattus norvegicus (Norway rat)	Rbbp4	NP_001101382.1
Xenopus tropicalis (tropical clawed frog)	rbbp4	NP_001011394.2
	RBBP4	XP_001104493.1
Caenorhabditis elegans (roundworm)	lin-53	NP_492552.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CCR4 and CAF1, the two deadenylases in the CCR4-NOT complex, can remove 3' terminal non-A residues in an exonucleolytic manner.
Title: CCR4 and CAF1 deadenylases have an intrinsic activity to remove the post-poly(A) sequence.

CAF-1 promotes Notch signaling through epigenetic control of target gene expression during Drosophila development.
Title: CAF-1 promotes Notch signaling through epigenetic control of target gene expression during Drosophila development.

CAF1-p180 and CAF1-p75 subunits mediate assembly of two different forms of chromatin.
Title: Subunits of the histone chaperone CAF1 also mediate assembly of protamine-based chromatin.

these studies suggest that p55 is not crucial for PRC2-mediated gene silencing in vivo, and the vital function of p55 is probably not dependent on its interaction with histone H4.
Title: The biological function of the WD40 repeat-containing protein p55/Caf1 in Drosophila.

Chromatin-modifying complex component Nurf55/p55 associates with histones H3 and H4 and polycomb repressive complex 2 subunit Su(z)12 through partially overlapping binding sites.
Title: Chromatin-modifying complex component Nurf55/p55 associates with histones H3 and H4 and polycomb repressive complex 2 subunit Su(z)12 through partially overlapping binding sites.

The following Caf1-55 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Caf1-55 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04943	NM_079630.4 Latest version!	Drosophila melanogaster chromatin assembly factor 1, p55 subunit, transcript variant A (Caf1-55), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07795	NM_001170146.2 Latest version!	Drosophila melanogaster chromatin assembly factor 1, p55 subunit, transcript variant B (Caf1-55), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Caf1-55 ( NM_079630.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524354.1 Drosophila melanogaster chromatin assembly factor 1, p55 subunit, transcript variant A (Caf1-55), mRNA.

Price & Availability↑

CloneID	OFa04943
Clone ID Related Accession (Same CDS sequence)	NM_079630.4
Accession Version	NM_079630.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chromatin assembly factor 1, p55 subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079630.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGGATC GCAGCGATAA TGCAGCGGAA TCCTTCGACG ACGCCGTCGA GGAGCGCGTG 
ATCAATGAGG AGTATAAGAT TTGGAAAAAG AACACGCCGT TCCTGTACGA TCTGGTCATG 
ACGCATGCCC TGGAATGGCC CTCGTTGACT GCCCAGTGGC TGCCGGACGT GACCAAGCAG 
GATGGCAAGG ACTACTCGGT ACATCGTCTT ATACTGGGCA CACACACATC TGACGAGCAG 
AACCATCTAC TCATCGCCAG CGTTCAGTTG CCCAGCGAGG ATGCCCAGTT CGATGGTTCG 
CACTACGATA ACGAGAAGGG CGAGTTTGGA GGCTTTGGAT CGGTGTGCGG CAAAATCGAA 
ATCGAGATTA AGATCAACCA CGAGGGCGAA GTGAACAGGG CCCGCTACAT GCCCCAGAAC 
GCCTGTGTAA TCGCCACCAA GACGCCGTCG AGTGATGTCC TGGTCTTCGA CTACACGAAG 
CATCCCAGCA AGCCGGAACC GTCTGGCGAG TGCCAGCCTG ACCTGCGGCT GCGCGGTCAT 
CAAAAGGAAG GCTACGGACT CTCTTGGAAT CCCAACCTCA ATGGCTATTT GCTGTCAGCC 
TCTGATGATC ACACCATCTG CTTGTGGGAC ATCAATGCCA CACCCAAGGA GCATAGGGTT 
ATTGATGCCA AGAACATCTT CACCGGCCAC ACCGCCGTTG TTGAGGACGT GGCTTGGCAT 
CTGCTACATG AGTCTCTGTT CGGATCGGTG GCTGACGACC AGAAGCTGAT GATCTGGGAC 
ACGCGCAACA ACAACACCTC GAAGCCCTCG CATACAGTGG ACGCTCACAC AGCGGAAGTC 
AATTGCTTGA GCTTTAATCC CTACTCGGAG TTCATTCTGG CCACGGGCTC CGCTGACAAG 
ACTGTAGCTC TATGGGATCT GCGCAACCTC AAGCTAAAAC TACATTCCTT CGAGTCGCAC 
AAGGACGAGA TCTTCCAAGT GCAGTGGTCG CCGCATAACG AGACTATTCT CGCCTCCTCC 
GGCACTGACC GCCGCCTACA CGTCTGGGAT TTGTCCAAAA TCGGCGAGGA GCAGAGTACG 
GAGGATGCTG AGGACGGACC ACCCGAGCTG CTCTTCATCC ATGGCGGTCA CACTGCCAAG 
ATTAGCGATT TCTCGTGGAA CCCCAATGAG CCATGGATCA TTTGCTCCGT GTCCGAGGAC 
AATATTATGC AGGTCTGGCA GATGGCCGAG AATGTTTACA ACGACGAGGA GCCAGAGATT 
CCCGCCTCCG AGTTAGAAAC CAATACCGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524354.1
CDS	318..1610
Translation	MVDRSDNAAESFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTKQDGKDYSVHRLILGTHTSDEQNHLLIASVQLPSEDAQFDGSHYDNEKGEFGGFGSVCGKIEIEIKINHEGEVNRARYMPQNACVIATKTPSSDVLVFDYTKHPSKPEPSGECQPDLRLRGHQKEGYGLSWNPNLNGYLLSASDDHTICLWDINATPKEHRVIDAKNIFTGHTAVVEDVAWHLLHESLFGSVADDQKLMIWDTRNNNTSKPSHTVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLHVWDLSKIGEEQSTEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWIICSVSEDNIMQVWQMAENVYNDEEPEIPASELETNTA

Target ORF information:

RefSeq Version	NM_079630.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chromatin assembly factor 1, p55 subunit, transcript variant A (Caf1-55), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079630.4

ORF Insert Sequence:

ATGGTGGATC GCAGCGATAA TGCAGCGGAA TCCTTCGACG ACGCCGTCGA GGAGCGCGTG 
ATCAATGAGG AGTATAAGAT TTGGAAAAAG AACACGCCGT TCCTGTACGA TCTGGTCATG 
ACGCATGCCC TGGAATGGCC CTCGTTGACT GCCCAGTGGC TGCCGGACGT GACCAAGCAG 
GATGGCAAGG ACTACTCGGT ACATCGTCTT ATACTGGGCA CACACACATC TGACGAGCAG 
AACCATCTAC TCATCGCCAG CGTTCAGTTG CCCAGCGAGG ATGCCCAGTT CGATGGTTCG 
CACTACGATA ACGAGAAGGG CGAGTTTGGA GGCTTTGGAT CGGTGTGCGG CAAAATCGAA 
ATCGAGATTA AGATCAACCA CGAGGGCGAA GTGAACAGGG CCCGCTACAT GCCCCAGAAC 
GCCTGTGTAA TCGCCACCAA GACGCCGTCG AGTGATGTCC TGGTCTTCGA CTACACGAAG 
CATCCCAGCA AGCCGGAACC GTCTGGCGAG TGCCAGCCTG ACCTGCGGCT GCGCGGTCAT 
CAAAAGGAAG GCTACGGACT CTCTTGGAAT CCCAACCTCA ATGGCTATTT GCTGTCAGCC 
TCTGATGATC ACACCATCTG CTTGTGGGAC ATCAATGCCA CACCCAAGGA GCATAGGGTT 
ATTGATGCCA AGAACATCTT CACCGGCCAC ACCGCCGTTG TTGAGGACGT GGCTTGGCAT 
CTGCTACATG AGTCTCTGTT CGGATCGGTG GCTGACGACC AGAAGCTGAT GATCTGGGAC 
ACGCGCAACA ACAACACCTC GAAGCCCTCG CATACAGTGG ACGCTCACAC AGCGGAAGTC 
AATTGCTTGA GCTTTAATCC CTACTCGGAG TTCATTCTGG CCACGGGCTC CGCTGACAAG 
ACTGTAGCTC TATGGGATCT GCGCAACCTC AAGCTAAAAC TACATTCCTT CGAGTCGCAC 
AAGGACGAGA TCTTCCAAGT GCAGTGGTCG CCGCATAACG AGACTATTCT CGCCTCCTCC 
GGCACTGACC GCCGCCTACA CGTCTGGGAT TTGTCCAAAA TCGGCGAGGA GCAGAGTACG 
GAGGATGCTG AGGACGGACC ACCCGAGCTG CTCTTCATCC ATGGCGGTCA CACTGCCAAG 
ATTAGCGATT TCTCGTGGAA CCCCAATGAG CCATGGATCA TTTGCTCCGT GTCCGAGGAC 
AATATTATGC AGGTCTGGCA GATGGCCGAG AATGTTTACA ACGACGAGGA GCCAGAGATT 
CCCGCCTCCG AGTTAGAAAC CAATACCGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Caf1-55 ( NM_001170146.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163617.1 Drosophila melanogaster chromatin assembly factor 1, p55 subunit, transcript variant B (Caf1-55), mRNA.

Price & Availability↑

CloneID	OFa07795
Clone ID Related Accession (Same CDS sequence)	NM_001170146.2
Accession Version	NM_001170146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chromatin assembly factor 1, p55 subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170146.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGGATC GCAGCGATAA TGCGGAATCC TTCGACGACG CCGTCGAGGA GCGCGTGATC 
AATGAGGAGT ATAAGATTTG GAAAAAGAAC ACGCCGTTCC TGTACGATCT GGTCATGACG 
CATGCCCTGG AATGGCCCTC GTTGACTGCC CAGTGGCTGC CGGACGTGAC CAAGCAGGAT 
GGCAAGGACT ACTCGGTACA TCGTCTTATA CTGGGCACAC ACACATCTGA CGAGCAGAAC 
CATCTACTCA TCGCCAGCGT TCAGTTGCCC AGCGAGGATG CCCAGTTCGA TGGTTCGCAC 
TACGATAACG AGAAGGGCGA GTTTGGAGGC TTTGGATCGG TGTGCGGCAA AATCGAAATC 
GAGATTAAGA TCAACCACGA GGGCGAAGTG AACAGGGCCC GCTACATGCC CCAGAACGCC 
TGTGTAATCG CCACCAAGAC GCCGTCGAGT GATGTCCTGG TCTTCGACTA CACGAAGCAT 
CCCAGCAAGC CGGAACCGTC TGGCGAGTGC CAGCCTGACC TGCGGCTGCG CGGTCATCAA 
AAGGAAGGCT ACGGACTCTC TTGGAATCCC AACCTCAATG GCTATTTGCT GTCAGCCTCT 
GATGATCACA CCATCTGCTT GTGGGACATC AATGCCACAC CCAAGGAGCA TAGGGTTATT 
GATGCCAAGA ACATCTTCAC CGGCCACACC GCCGTTGTTG AGGACGTGGC TTGGCATCTG 
CTACATGAGT CTCTGTTCGG ATCGGTGGCT GACGACCAGA AGCTGATGAT CTGGGACACG 
CGCAACAACA ACACCTCGAA GCCCTCGCAT ACAGTGGACG CTCACACAGC GGAAGTCAAT 
TGCTTGAGCT TTAATCCCTA CTCGGAGTTC ATTCTGGCCA CGGGCTCCGC TGACAAGACT 
GTAGCTCTAT GGGATCTGCG CAACCTCAAG CTAAAACTAC ATTCCTTCGA GTCGCACAAG 
GACGAGATCT TCCAAGTGCA GTGGTCGCCG CATAACGAGA CTATTCTCGC CTCCTCCGGC 
ACTGACCGCC GCCTACACGT CTGGGATTTG TCCAAAATCG GCGAGGAGCA GAGTACGGAG 
GATGCTGAGG ACGGACCACC CGAGCTGCTC TTCATCCATG GCGGTCACAC TGCCAAGATT 
AGCGATTTCT CGTGGAACCC CAATGAGCCA TGGATCATTT GCTCCGTGTC CGAGGACAAT 
ATTATGCAGG TCTGGCAGAT GGCCGAGAAT GTTTACAACG ACGAGGAGCC AGAGATTCCC 
GCCTCCGAGT TAGAAACCAA TACCGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163617.1
CDS	318..1607
Translation	MVDRSDNAESFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTKQDGKDYSVHRLILGTHTSDEQNHLLIASVQLPSEDAQFDGSHYDNEKGEFGGFGSVCGKIEIEIKINHEGEVNRARYMPQNACVIATKTPSSDVLVFDYTKHPSKPEPSGECQPDLRLRGHQKEGYGLSWNPNLNGYLLSASDDHTICLWDINATPKEHRVIDAKNIFTGHTAVVEDVAWHLLHESLFGSVADDQKLMIWDTRNNNTSKPSHTVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLHVWDLSKIGEEQSTEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWIICSVSEDNIMQVWQMAENVYNDEEPEIPASELETNTA

Target ORF information:

RefSeq Version	NM_001170146.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chromatin assembly factor 1, p55 subunit, transcript variant B (Caf1-55), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170146.2

ORF Insert Sequence:

ATGGTGGATC GCAGCGATAA TGCGGAATCC TTCGACGACG CCGTCGAGGA GCGCGTGATC 
AATGAGGAGT ATAAGATTTG GAAAAAGAAC ACGCCGTTCC TGTACGATCT GGTCATGACG 
CATGCCCTGG AATGGCCCTC GTTGACTGCC CAGTGGCTGC CGGACGTGAC CAAGCAGGAT 
GGCAAGGACT ACTCGGTACA TCGTCTTATA CTGGGCACAC ACACATCTGA CGAGCAGAAC 
CATCTACTCA TCGCCAGCGT TCAGTTGCCC AGCGAGGATG CCCAGTTCGA TGGTTCGCAC 
TACGATAACG AGAAGGGCGA GTTTGGAGGC TTTGGATCGG TGTGCGGCAA AATCGAAATC 
GAGATTAAGA TCAACCACGA GGGCGAAGTG AACAGGGCCC GCTACATGCC CCAGAACGCC 
TGTGTAATCG CCACCAAGAC GCCGTCGAGT GATGTCCTGG TCTTCGACTA CACGAAGCAT 
CCCAGCAAGC CGGAACCGTC TGGCGAGTGC CAGCCTGACC TGCGGCTGCG CGGTCATCAA 
AAGGAAGGCT ACGGACTCTC TTGGAATCCC AACCTCAATG GCTATTTGCT GTCAGCCTCT 
GATGATCACA CCATCTGCTT GTGGGACATC AATGCCACAC CCAAGGAGCA TAGGGTTATT 
GATGCCAAGA ACATCTTCAC CGGCCACACC GCCGTTGTTG AGGACGTGGC TTGGCATCTG 
CTACATGAGT CTCTGTTCGG ATCGGTGGCT GACGACCAGA AGCTGATGAT CTGGGACACG 
CGCAACAACA ACACCTCGAA GCCCTCGCAT ACAGTGGACG CTCACACAGC GGAAGTCAAT 
TGCTTGAGCT TTAATCCCTA CTCGGAGTTC ATTCTGGCCA CGGGCTCCGC TGACAAGACT 
GTAGCTCTAT GGGATCTGCG CAACCTCAAG CTAAAACTAC ATTCCTTCGA GTCGCACAAG 
GACGAGATCT TCCAAGTGCA GTGGTCGCCG CATAACGAGA CTATTCTCGC CTCCTCCGGC 
ACTGACCGCC GCCTACACGT CTGGGATTTG TCCAAAATCG GCGAGGAGCA GAGTACGGAG 
GATGCTGAGG ACGGACCACC CGAGCTGCTC TTCATCCATG GCGGTCACAC TGCCAAGATT 
AGCGATTTCT CGTGGAACCC CAATGAGCCA TGGATCATTT GCTCCGTGTC CGAGGACAAT 
ATTATGCAGG TCTGGCAGAT GGCCGAGAAT GTTTACAACG ACGAGGAGCC AGAGATTCCC 
GCCTCCGAGT TAGAAACCAA TACCGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Caf1-55 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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