Gyc88E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gyc88E
Entrez Gene ID	41825
Full Name	Guanylyl cyclase at 88E
Synonyms	CG4154,Dmel\CG4154,GYC,Gyc-88E
General protein information	Preferred Names Guanylyl cyclase at 88E Names CG4154 gene product from transcript CG4154-RD CG4154-PD CG4154-PE CG4154-PF Gyc88E-PD Gyc88E-PE Gyc88E-PF guanylyl cyclase at 88E
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88E2-88E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260186.2	NP_001247115.1	guanylyl cyclase at 88E, isoform E
NM_001043246.4	NP_001036711.1	guanylyl cyclase at 88E, isoform D
NM_169616.4	NP_731974.4	guanylyl cyclase at 88E, isoform F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Gyc88E	NP_001036711.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001985	XP_321074.5
Caenorhabditis elegans (roundworm)	gcy-31	NP_001024889.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

biochemical properties of Gyc-88E are unique for guanylate cyclases and suggest a possible function as an oxygen sensor
Title: Ligand binding and inhibition of an oxygen-sensitive soluble guanylate cyclase, Gyc-88E, from Drosophila.

Mutants of NO-dependent cyclase show a specifically-marked behaviour phenotype, but on the other hand they are associated with moderate biochemical defects.
Title: Exploratory behaviour in NO-dependent cyclase mutants of Drosophila shows defects in coincident neuronal signalling.

Gyc-88E and Gyc-89Db are co-expressed in a subset of sensory neurons where they would be ideally situated to act as oxygen sensors
Title: Atypical soluble guanylyl cyclases in Drosophila can function as molecular oxygen sensors.

The following Gyc88E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gyc88E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04933	NM_001043246.4 Latest version!	Drosophila melanogaster guanylyl cyclase at 88E, transcript variant D (Gyc88E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa04933	NM_001260186.2 Latest version!	Drosophila melanogaster guanylyl cyclase at 88E, transcript variant E (Gyc88E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa04934	NM_169616.4 Latest version!	Drosophila melanogaster guanylyl cyclase at 88E, transcript variant F (Gyc88E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gyc88E ( NM_001260186.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247115.1 Drosophila melanogaster guanylyl cyclase at 88E, transcript variant E (Gyc88E), mRNA.

Price & Availability↑

CloneID	OFa04933
Clone ID Related Accession (Same CDS sequence)	NM_001260186.2 , NM_001043246.4
Accession Version	NM_001260186.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 88E, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260186.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACGGAC TGCTGCTGGA GAACCTCTCC GAGTACATCA AGTCCGTTTA CGGCGAGGAG 
AAATGGGAGG ACATCCGGCG ACAGGCTGGC ATCGATTCGC CCTCCTTCAG CGTCCATCAG 
GTCTATCCCG AGAATCTGCT GCAGAAGCTG GCGAAAAAGG CTCAACAGGT TCTGGGCGTT 
TCTGAGCGGG ATTTCATGGA CCAGATGGGC GTCTACTTCG TGGGCTTTGT GGGCCAGTAT 
GGCTACGATC GTGTCCTTTC CGTCCTGGGC CGCCACATGC GCGACTTTCT CAACGGACTG 
GACAACCTGC ATGAGTACCT GAAGTTCTCG TATCCGCGGA TGAGAGCTCC CAGCTTTATC 
TGCGAAAACG AAACGAAGCA GGGTCTGACC CTGCACTATC GTTCCAAGCG TCGAGGATTC 
GTCTACTACA CGATGGGTCA AATCCGAGAG GTGGCACGGT ATTTCTATCA CAAGGAGATG 
CATATTGAGC TCGTTCGGGA AGAGATCCTC TTTGACACGG TCCATGTGAC CTTCCAACTG 
ACCTTCGATA ATCGGGCCTT CACACTTGCA TCCTTGGCCA TGACCCGGGA GGAGAAACAT 
CTGCCCATCA GTGCTCATGT GCTGTTTGAG ATATTTCCCT TTTGCATGGT TTTTGGTGCT 
GATATGGTTG TGCGTAGTAT TGGTAACTCT TTGATGGTGA TTTTACCGGA ACTTCTGGGA 
AAAAAAATTA CGGCCTGGTT CGATCTAGTT CGTCCCTTAA TTGCATTCAA GTTTCAGACT 
ATACTCAATC GTACTAACAA CATTTTCGAG CTGGTCACTG TGGATCCAGT TACGGAGAGA 
TTCGATGTGC AAAACGAGGA CTTACTGCAG CATGAAGATG GCAGCGAACC GGAAAAGTCT 
CTTAGATTAA AAGGTCAAAT GGTTTACATG GAGAACTGGC GCATGATTAT GTTTCTGGGT 
ACTCCCGTAA TGCCGGATCT GACCTCACTA ATAACCACAG GTCTATATAT CAACGATCTG 
TCCATGCACG ACTTCAGCAG AGATCTCATG CTGGCGGGCA CTCAACAATC GGTTGAACTG 
AAGTTGGCAC TGGATCAAGA ACAGCAGAAG TCAAAGAAGC TAGAGGAGTC CATGAGATTG 
TTGGATGAGG AGATGCGTAG AACGGATGAG CTGCTGTATC AGATGATACC CAAACAGGTG 
GCTGATAGGC TCCGACGGGG CGAGAATCCC ATTGATACCT GTGAGATGTT CGATAGCGTT 
TCCATCCTGT TCTCAGACAT CGTTACCTTC ACCGAGATTT GCAGCCGCAT TACTCCGATG 
GAGGTAGTGT CCATGCTGAA CGCTATGTAC TCCATTTTCG ATAAGCTGAC GGAGCGGAAT 
TCAGTGTACA AGGTGGAAAC TATTGGTGAT GCGTATATGG TGGTGGCGGG TGCTCCGGAT 
AAGGATGCCA ATCACGCCGA GCGAGTCTGT GATATGGCCC TGGATATGGT GGATGCGATA 
ACCGATCTAA AGGATCCCTC AACGGGCCAG CACTTGAGGA TACGTGTGGG CGTCCACTCG 
GGCGCAGTGG TGGCGGGCAT CGTGGGCCTT AAGATGCCGC GTTACTGTCT CTTTGGCGAT 
ACGGTCAATA CCGCCTCACG AATGGAGTCC ACCAGCATCG CCATGAAGGT GCACATATCC 
GAGTCTACGA AGGTTCTTAT TGGACCCAAC TACAAGATCA TCGAGCGCGG TGAGATCGAT 
GTAAAGGGCA AGGGCACCAT GGGCACATAC TGGCTGGAGG AACGAGAGAA CCGGCTGCCT 
CTGCAACTAA CCGCTGCTCT GCAAGTTCAT CCGCTCTCAC CTGTCCCACC GACGCCCACA 
CCCAAGACGA AGGCCATCAT GCCTCCTGTA TCCAAGCCGT TGACTCCGAT GATGCCCGTT 
TCAGTTTCCC TGGCAGCTTC CATGCCCACC AGCAACGTTC CTGCTGTGGA TGTGATGGCT 
TCATCATCCA GCATTTCCGG TTTGGCACTA ACAGCCGCTG CAGCGGCCCA CATGTCGCTA 
CATCACCAGG CGGTGGTGGC GGAGGCATTA ACTGGAGCTT CAGTTGAAGT GGCACTACCA 
TCAGTAGCTT CTGGCGCAAC AGGAGCGGCA GCGGGTGGAG GTGCACCGTC GGATGACCGC 
AACAGTCGCA TCTACTCCCC CGTGACCTTC AAGGATGTGG CTCGACGAAG CGTAGCCAAT 
TCTCCGGTAA GGAGCTGTGC TCAACCGGAT CAGGAGAGAC GTCGCGAGTC CCGCTCCAAT 
TCCACGGGCC ACGTGTTTAT GCGAACTCCT TCGGAAATTT TCGGCTCTCT CATCCTGGAC 
ACCGAGGAGT TTCTCGAGGA TCTGCAAATC TCACGTTCCT CCCTGGCAAA CAATAACAAC 
AATCAGAGCC CCTGCGGTTT TAGCCCAACT CCTCCATTCC GGATTGGCAG CGCACCGCCC 
AAGCCGAGAC CTAGTAATCC GGATAAATTT ACACCGGAGG AACTGGCCGC CATGGATCAA 
CTGACGCCTC CGTCAACGGC ACCAGCCAGA GAAACGGCCA GCTGCAGCAG TGCATCATTG 
GATCGCGACA AGGCGACCAA GCTGAAAAAA ATCACTTTCT CCAACAGCAG CAGTCTGGAT 
GCAACCACTC CAACTGCGTT GGCTGCTGTG GTATGTCCAA TGAGAACCAA GTCACCGCCA 
ATGGCAGTGG CTCCAGTGAT GCACATGGTA CAGGCTAGCA CCAGCAAAGG CGACGGCCAA 
CGACCAGGCT CGAAGGATTC AGTATCATCC ATTTCCCTGC ACTCACCTCC TCCGCATCGA 
TCTAACTCGG CACCAGCCAG ACCGCACTCA ATGTCGAAGG CGGCGCGAAA GGCCTTCCTG 
GCCGCCAAAC AGACCAAGGC AATGGAAAAG CTGGACAAGA TGATCGAAGA GGTTCAAGAA 
GTGGAGTCTC AGTCGGTGGC AAAGGCGGCC AACATGCGTC TGGCCCTTTA CGGCCACGAT 
GGAGGTGGTG ATTTGGCTGC CGGTGGGTGT CCACTGTTTC TGCCTCCGCC GCCGCAGCAG 
CAGCAGCAGC AGCAACAGAG CCTGATGCCC AGCTCCATCT CAGACTCCGG CCTCTGCAGT 
CATGGTCATA GTCATGCACC CAGCTGTCAT CATATGGATC CGAAGATGAC CAACAGCCAG 
AGTTTCCAGC ATTCTCCACG TGGCGGAATA ACCCATCAGT GCTGCAGCGG CTTTGGACAT 
GGCAATGGAC GTCATTCGCA CCGGATGCAC TCGAATGCTT GCCGGATTCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247115.1
CDS	361..3654
Translation	MYGLLLENLSEYIKSVYGEEKWEDIRRQAGIDSPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQYGYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEMHIELVREEILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLPISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLGKKITAWFDLVRPLIAFKFQTILNRTNNIFELVTVDPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLGTPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKKLEESMRLLDEEMRRTDELLYQMIPKQVADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPDKDANHAERVCDMALDMVDAITDLKDPSTGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHISESTKVLIGPNYKIIERGEIDVKGKGTMGTYWLEERENRLPLQLTAALQVHPLSPVPPTPTPKTKAIMPPVSKPLTPMMPVSVSLAASMPTSNVPAVDVMASSSSISGLALTAAAAAHMSLHHQAVVAEALTGASVEVALPSVASGATGAAAGGGAPSDDRNSRIYSPVTFKDVARRSVANSPVRSCAQPDQERRRESRSNSTGHVFMRTPSEIFGSLILDTEEFLEDLQISRSSLANNNNNQSPCGFSPTPPFRIGSAPPKPRPSNPDKFTPEELAAMDQLTPPSTAPARETASCSSASLDRDKATKLKKITFSNSSSLDATTPTALAAVVCPMRTKSPPMAVAPVMHMVQASTSKGDGQRPGSKDSVSSISLHSPPPHRSNSAPARPHSMSKAARKAFLAAKQTKAMEKLDKMIEEVQEVESQSVAKAANMRLALYGHDGGGDLAAGGCPLFLPPPPQQQQQQQQSLMPSSISDSGLCSHGHSHAPSCHHMDPKMTNSQSFQHSPRGGITHQCCSGFGHGNGRHSHRMHSNACRIL

Target ORF information:

RefSeq Version	NM_001260186.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 88E, transcript variant E (Gyc88E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260186.2

ORF Insert Sequence:

ATGTACGGAC TGCTGCTGGA GAACCTCTCC GAGTACATCA AGTCCGTTTA CGGCGAGGAG 
AAATGGGAGG ACATCCGGCG ACAGGCTGGC ATCGATTCGC CCTCCTTCAG CGTCCATCAG 
GTCTATCCCG AGAATCTGCT GCAGAAGCTG GCGAAAAAGG CTCAACAGGT TCTGGGCGTT 
TCTGAGCGGG ATTTCATGGA CCAGATGGGC GTCTACTTCG TGGGCTTTGT GGGCCAGTAT 
GGCTACGATC GTGTCCTTTC CGTCCTGGGC CGCCACATGC GCGACTTTCT CAACGGACTG 
GACAACCTGC ATGAGTACCT GAAGTTCTCG TATCCGCGGA TGAGAGCTCC CAGCTTTATC 
TGCGAAAACG AAACGAAGCA GGGTCTGACC CTGCACTATC GTTCCAAGCG TCGAGGATTC 
GTCTACTACA CGATGGGTCA AATCCGAGAG GTGGCACGGT ATTTCTATCA CAAGGAGATG 
CATATTGAGC TCGTTCGGGA AGAGATCCTC TTTGACACGG TCCATGTGAC CTTCCAACTG 
ACCTTCGATA ATCGGGCCTT CACACTTGCA TCCTTGGCCA TGACCCGGGA GGAGAAACAT 
CTGCCCATCA GTGCTCATGT GCTGTTTGAG ATATTTCCCT TTTGCATGGT TTTTGGTGCT 
GATATGGTTG TGCGTAGTAT TGGTAACTCT TTGATGGTGA TTTTACCGGA ACTTCTGGGA 
AAAAAAATTA CGGCCTGGTT CGATCTAGTT CGTCCCTTAA TTGCATTCAA GTTTCAGACT 
ATACTCAATC GTACTAACAA CATTTTCGAG CTGGTCACTG TGGATCCAGT TACGGAGAGA 
TTCGATGTGC AAAACGAGGA CTTACTGCAG CATGAAGATG GCAGCGAACC GGAAAAGTCT 
CTTAGATTAA AAGGTCAAAT GGTTTACATG GAGAACTGGC GCATGATTAT GTTTCTGGGT 
ACTCCCGTAA TGCCGGATCT GACCTCACTA ATAACCACAG GTCTATATAT CAACGATCTG 
TCCATGCACG ACTTCAGCAG AGATCTCATG CTGGCGGGCA CTCAACAATC GGTTGAACTG 
AAGTTGGCAC TGGATCAAGA ACAGCAGAAG TCAAAGAAGC TAGAGGAGTC CATGAGATTG 
TTGGATGAGG AGATGCGTAG AACGGATGAG CTGCTGTATC AGATGATACC CAAACAGGTG 
GCTGATAGGC TCCGACGGGG CGAGAATCCC ATTGATACCT GTGAGATGTT CGATAGCGTT 
TCCATCCTGT TCTCAGACAT CGTTACCTTC ACCGAGATTT GCAGCCGCAT TACTCCGATG 
GAGGTAGTGT CCATGCTGAA CGCTATGTAC TCCATTTTCG ATAAGCTGAC GGAGCGGAAT 
TCAGTGTACA AGGTGGAAAC TATTGGTGAT GCGTATATGG TGGTGGCGGG TGCTCCGGAT 
AAGGATGCCA ATCACGCCGA GCGAGTCTGT GATATGGCCC TGGATATGGT GGATGCGATA 
ACCGATCTAA AGGATCCCTC AACGGGCCAG CACTTGAGGA TACGTGTGGG CGTCCACTCG 
GGCGCAGTGG TGGCGGGCAT CGTGGGCCTT AAGATGCCGC GTTACTGTCT CTTTGGCGAT 
ACGGTCAATA CCGCCTCACG AATGGAGTCC ACCAGCATCG CCATGAAGGT GCACATATCC 
GAGTCTACGA AGGTTCTTAT TGGACCCAAC TACAAGATCA TCGAGCGCGG TGAGATCGAT 
GTAAAGGGCA AGGGCACCAT GGGCACATAC TGGCTGGAGG AACGAGAGAA CCGGCTGCCT 
CTGCAACTAA CCGCTGCTCT GCAAGTTCAT CCGCTCTCAC CTGTCCCACC GACGCCCACA 
CCCAAGACGA AGGCCATCAT GCCTCCTGTA TCCAAGCCGT TGACTCCGAT GATGCCCGTT 
TCAGTTTCCC TGGCAGCTTC CATGCCCACC AGCAACGTTC CTGCTGTGGA TGTGATGGCT 
TCATCATCCA GCATTTCCGG TTTGGCACTA ACAGCCGCTG CAGCGGCCCA CATGTCGCTA 
CATCACCAGG CGGTGGTGGC GGAGGCATTA ACTGGAGCTT CAGTTGAAGT GGCACTACCA 
TCAGTAGCTT CTGGCGCAAC AGGAGCGGCA GCGGGTGGAG GTGCACCGTC GGATGACCGC 
AACAGTCGCA TCTACTCCCC CGTGACCTTC AAGGATGTGG CTCGACGAAG CGTAGCCAAT 
TCTCCGGTAA GGAGCTGTGC TCAACCGGAT CAGGAGAGAC GTCGCGAGTC CCGCTCCAAT 
TCCACGGGCC ACGTGTTTAT GCGAACTCCT TCGGAAATTT TCGGCTCTCT CATCCTGGAC 
ACCGAGGAGT TTCTCGAGGA TCTGCAAATC TCACGTTCCT CCCTGGCAAA CAATAACAAC 
AATCAGAGCC CCTGCGGTTT TAGCCCAACT CCTCCATTCC GGATTGGCAG CGCACCGCCC 
AAGCCGAGAC CTAGTAATCC GGATAAATTT ACACCGGAGG AACTGGCCGC CATGGATCAA 
CTGACGCCTC CGTCAACGGC ACCAGCCAGA GAAACGGCCA GCTGCAGCAG TGCATCATTG 
GATCGCGACA AGGCGACCAA GCTGAAAAAA ATCACTTTCT CCAACAGCAG CAGTCTGGAT 
GCAACCACTC CAACTGCGTT GGCTGCTGTG GTATGTCCAA TGAGAACCAA GTCACCGCCA 
ATGGCAGTGG CTCCAGTGAT GCACATGGTA CAGGCTAGCA CCAGCAAAGG CGACGGCCAA 
CGACCAGGCT CGAAGGATTC AGTATCATCC ATTTCCCTGC ACTCACCTCC TCCGCATCGA 
TCTAACTCGG CACCAGCCAG ACCGCACTCA ATGTCGAAGG CGGCGCGAAA GGCCTTCCTG 
GCCGCCAAAC AGACCAAGGC AATGGAAAAG CTGGACAAGA TGATCGAAGA GGTTCAAGAA 
GTGGAGTCTC AGTCGGTGGC AAAGGCGGCC AACATGCGTC TGGCCCTTTA CGGCCACGAT 
GGAGGTGGTG ATTTGGCTGC CGGTGGGTGT CCACTGTTTC TGCCTCCGCC GCCGCAGCAG 
CAGCAGCAGC AGCAACAGAG CCTGATGCCC AGCTCCATCT CAGACTCCGG CCTCTGCAGT 
CATGGTCATA GTCATGCACC CAGCTGTCAT CATATGGATC CGAAGATGAC CAACAGCCAG 
AGTTTCCAGC ATTCTCCACG TGGCGGAATA ACCCATCAGT GCTGCAGCGG CTTTGGACAT 
GGCAATGGAC GTCATTCGCA CCGGATGCAC TCGAATGCTT GCCGGATTCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gyc88E ( NM_001043246.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036711.1 Drosophila melanogaster guanylyl cyclase at 88E, transcript variant D (Gyc88E), mRNA.

Price & Availability↑

CloneID	OFa04933
Clone ID Related Accession (Same CDS sequence)	NM_001260186.2 , NM_001043246.4
Accession Version	NM_001043246.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 88E, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001043246.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACGGAC TGCTGCTGGA GAACCTCTCC GAGTACATCA AGTCCGTTTA CGGCGAGGAG 
AAATGGGAGG ACATCCGGCG ACAGGCTGGC ATCGATTCGC CCTCCTTCAG CGTCCATCAG 
GTCTATCCCG AGAATCTGCT GCAGAAGCTG GCGAAAAAGG CTCAACAGGT TCTGGGCGTT 
TCTGAGCGGG ATTTCATGGA CCAGATGGGC GTCTACTTCG TGGGCTTTGT GGGCCAGTAT 
GGCTACGATC GTGTCCTTTC CGTCCTGGGC CGCCACATGC GCGACTTTCT CAACGGACTG 
GACAACCTGC ATGAGTACCT GAAGTTCTCG TATCCGCGGA TGAGAGCTCC CAGCTTTATC 
TGCGAAAACG AAACGAAGCA GGGTCTGACC CTGCACTATC GTTCCAAGCG TCGAGGATTC 
GTCTACTACA CGATGGGTCA AATCCGAGAG GTGGCACGGT ATTTCTATCA CAAGGAGATG 
CATATTGAGC TCGTTCGGGA AGAGATCCTC TTTGACACGG TCCATGTGAC CTTCCAACTG 
ACCTTCGATA ATCGGGCCTT CACACTTGCA TCCTTGGCCA TGACCCGGGA GGAGAAACAT 
CTGCCCATCA GTGCTCATGT GCTGTTTGAG ATATTTCCCT TTTGCATGGT TTTTGGTGCT 
GATATGGTTG TGCGTAGTAT TGGTAACTCT TTGATGGTGA TTTTACCGGA ACTTCTGGGA 
AAAAAAATTA CGGCCTGGTT CGATCTAGTT CGTCCCTTAA TTGCATTCAA GTTTCAGACT 
ATACTCAATC GTACTAACAA CATTTTCGAG CTGGTCACTG TGGATCCAGT TACGGAGAGA 
TTCGATGTGC AAAACGAGGA CTTACTGCAG CATGAAGATG GCAGCGAACC GGAAAAGTCT 
CTTAGATTAA AAGGTCAAAT GGTTTACATG GAGAACTGGC GCATGATTAT GTTTCTGGGT 
ACTCCCGTAA TGCCGGATCT GACCTCACTA ATAACCACAG GTCTATATAT CAACGATCTG 
TCCATGCACG ACTTCAGCAG AGATCTCATG CTGGCGGGCA CTCAACAATC GGTTGAACTG 
AAGTTGGCAC TGGATCAAGA ACAGCAGAAG TCAAAGAAGC TAGAGGAGTC CATGAGATTG 
TTGGATGAGG AGATGCGTAG AACGGATGAG CTGCTGTATC AGATGATACC CAAACAGGTG 
GCTGATAGGC TCCGACGGGG CGAGAATCCC ATTGATACCT GTGAGATGTT CGATAGCGTT 
TCCATCCTGT TCTCAGACAT CGTTACCTTC ACCGAGATTT GCAGCCGCAT TACTCCGATG 
GAGGTAGTGT CCATGCTGAA CGCTATGTAC TCCATTTTCG ATAAGCTGAC GGAGCGGAAT 
TCAGTGTACA AGGTGGAAAC TATTGGTGAT GCGTATATGG TGGTGGCGGG TGCTCCGGAT 
AAGGATGCCA ATCACGCCGA GCGAGTCTGT GATATGGCCC TGGATATGGT GGATGCGATA 
ACCGATCTAA AGGATCCCTC AACGGGCCAG CACTTGAGGA TACGTGTGGG CGTCCACTCG 
GGCGCAGTGG TGGCGGGCAT CGTGGGCCTT AAGATGCCGC GTTACTGTCT CTTTGGCGAT 
ACGGTCAATA CCGCCTCACG AATGGAGTCC ACCAGCATCG CCATGAAGGT GCACATATCC 
GAGTCTACGA AGGTTCTTAT TGGACCCAAC TACAAGATCA TCGAGCGCGG TGAGATCGAT 
GTAAAGGGCA AGGGCACCAT GGGCACATAC TGGCTGGAGG AACGAGAGAA CCGGCTGCCT 
CTGCAACTAA CCGCTGCTCT GCAAGTTCAT CCGCTCTCAC CTGTCCCACC GACGCCCACA 
CCCAAGACGA AGGCCATCAT GCCTCCTGTA TCCAAGCCGT TGACTCCGAT GATGCCCGTT 
TCAGTTTCCC TGGCAGCTTC CATGCCCACC AGCAACGTTC CTGCTGTGGA TGTGATGGCT 
TCATCATCCA GCATTTCCGG TTTGGCACTA ACAGCCGCTG CAGCGGCCCA CATGTCGCTA 
CATCACCAGG CGGTGGTGGC GGAGGCATTA ACTGGAGCTT CAGTTGAAGT GGCACTACCA 
TCAGTAGCTT CTGGCGCAAC AGGAGCGGCA GCGGGTGGAG GTGCACCGTC GGATGACCGC 
AACAGTCGCA TCTACTCCCC CGTGACCTTC AAGGATGTGG CTCGACGAAG CGTAGCCAAT 
TCTCCGGTAA GGAGCTGTGC TCAACCGGAT CAGGAGAGAC GTCGCGAGTC CCGCTCCAAT 
TCCACGGGCC ACGTGTTTAT GCGAACTCCT TCGGAAATTT TCGGCTCTCT CATCCTGGAC 
ACCGAGGAGT TTCTCGAGGA TCTGCAAATC TCACGTTCCT CCCTGGCAAA CAATAACAAC 
AATCAGAGCC CCTGCGGTTT TAGCCCAACT CCTCCATTCC GGATTGGCAG CGCACCGCCC 
AAGCCGAGAC CTAGTAATCC GGATAAATTT ACACCGGAGG AACTGGCCGC CATGGATCAA 
CTGACGCCTC CGTCAACGGC ACCAGCCAGA GAAACGGCCA GCTGCAGCAG TGCATCATTG 
GATCGCGACA AGGCGACCAA GCTGAAAAAA ATCACTTTCT CCAACAGCAG CAGTCTGGAT 
GCAACCACTC CAACTGCGTT GGCTGCTGTG GTATGTCCAA TGAGAACCAA GTCACCGCCA 
ATGGCAGTGG CTCCAGTGAT GCACATGGTA CAGGCTAGCA CCAGCAAAGG CGACGGCCAA 
CGACCAGGCT CGAAGGATTC AGTATCATCC ATTTCCCTGC ACTCACCTCC TCCGCATCGA 
TCTAACTCGG CACCAGCCAG ACCGCACTCA ATGTCGAAGG CGGCGCGAAA GGCCTTCCTG 
GCCGCCAAAC AGACCAAGGC AATGGAAAAG CTGGACAAGA TGATCGAAGA GGTTCAAGAA 
GTGGAGTCTC AGTCGGTGGC AAAGGCGGCC AACATGCGTC TGGCCCTTTA CGGCCACGAT 
GGAGGTGGTG ATTTGGCTGC CGGTGGGTGT CCACTGTTTC TGCCTCCGCC GCCGCAGCAG 
CAGCAGCAGC AGCAACAGAG CCTGATGCCC AGCTCCATCT CAGACTCCGG CCTCTGCAGT 
CATGGTCATA GTCATGCACC CAGCTGTCAT CATATGGATC CGAAGATGAC CAACAGCCAG 
AGTTTCCAGC ATTCTCCACG TGGCGGAATA ACCCATCAGT GCTGCAGCGG CTTTGGACAT 
GGCAATGGAC GTCATTCGCA CCGGATGCAC TCGAATGCTT GCCGGATTCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036711.1
CDS	455..3748
Translation	MYGLLLENLSEYIKSVYGEEKWEDIRRQAGIDSPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQYGYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEMHIELVREEILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLPISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLGKKITAWFDLVRPLIAFKFQTILNRTNNIFELVTVDPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLGTPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKKLEESMRLLDEEMRRTDELLYQMIPKQVADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPDKDANHAERVCDMALDMVDAITDLKDPSTGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHISESTKVLIGPNYKIIERGEIDVKGKGTMGTYWLEERENRLPLQLTAALQVHPLSPVPPTPTPKTKAIMPPVSKPLTPMMPVSVSLAASMPTSNVPAVDVMASSSSISGLALTAAAAAHMSLHHQAVVAEALTGASVEVALPSVASGATGAAAGGGAPSDDRNSRIYSPVTFKDVARRSVANSPVRSCAQPDQERRRESRSNSTGHVFMRTPSEIFGSLILDTEEFLEDLQISRSSLANNNNNQSPCGFSPTPPFRIGSAPPKPRPSNPDKFTPEELAAMDQLTPPSTAPARETASCSSASLDRDKATKLKKITFSNSSSLDATTPTALAAVVCPMRTKSPPMAVAPVMHMVQASTSKGDGQRPGSKDSVSSISLHSPPPHRSNSAPARPHSMSKAARKAFLAAKQTKAMEKLDKMIEEVQEVESQSVAKAANMRLALYGHDGGGDLAAGGCPLFLPPPPQQQQQQQQSLMPSSISDSGLCSHGHSHAPSCHHMDPKMTNSQSFQHSPRGGITHQCCSGFGHGNGRHSHRMHSNACRIL

Target ORF information:

RefSeq Version	NM_001043246.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 88E, transcript variant D (Gyc88E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043246.4

ORF Insert Sequence:

ATGTACGGAC TGCTGCTGGA GAACCTCTCC GAGTACATCA AGTCCGTTTA CGGCGAGGAG 
AAATGGGAGG ACATCCGGCG ACAGGCTGGC ATCGATTCGC CCTCCTTCAG CGTCCATCAG 
GTCTATCCCG AGAATCTGCT GCAGAAGCTG GCGAAAAAGG CTCAACAGGT TCTGGGCGTT 
TCTGAGCGGG ATTTCATGGA CCAGATGGGC GTCTACTTCG TGGGCTTTGT GGGCCAGTAT 
GGCTACGATC GTGTCCTTTC CGTCCTGGGC CGCCACATGC GCGACTTTCT CAACGGACTG 
GACAACCTGC ATGAGTACCT GAAGTTCTCG TATCCGCGGA TGAGAGCTCC CAGCTTTATC 
TGCGAAAACG AAACGAAGCA GGGTCTGACC CTGCACTATC GTTCCAAGCG TCGAGGATTC 
GTCTACTACA CGATGGGTCA AATCCGAGAG GTGGCACGGT ATTTCTATCA CAAGGAGATG 
CATATTGAGC TCGTTCGGGA AGAGATCCTC TTTGACACGG TCCATGTGAC CTTCCAACTG 
ACCTTCGATA ATCGGGCCTT CACACTTGCA TCCTTGGCCA TGACCCGGGA GGAGAAACAT 
CTGCCCATCA GTGCTCATGT GCTGTTTGAG ATATTTCCCT TTTGCATGGT TTTTGGTGCT 
GATATGGTTG TGCGTAGTAT TGGTAACTCT TTGATGGTGA TTTTACCGGA ACTTCTGGGA 
AAAAAAATTA CGGCCTGGTT CGATCTAGTT CGTCCCTTAA TTGCATTCAA GTTTCAGACT 
ATACTCAATC GTACTAACAA CATTTTCGAG CTGGTCACTG TGGATCCAGT TACGGAGAGA 
TTCGATGTGC AAAACGAGGA CTTACTGCAG CATGAAGATG GCAGCGAACC GGAAAAGTCT 
CTTAGATTAA AAGGTCAAAT GGTTTACATG GAGAACTGGC GCATGATTAT GTTTCTGGGT 
ACTCCCGTAA TGCCGGATCT GACCTCACTA ATAACCACAG GTCTATATAT CAACGATCTG 
TCCATGCACG ACTTCAGCAG AGATCTCATG CTGGCGGGCA CTCAACAATC GGTTGAACTG 
AAGTTGGCAC TGGATCAAGA ACAGCAGAAG TCAAAGAAGC TAGAGGAGTC CATGAGATTG 
TTGGATGAGG AGATGCGTAG AACGGATGAG CTGCTGTATC AGATGATACC CAAACAGGTG 
GCTGATAGGC TCCGACGGGG CGAGAATCCC ATTGATACCT GTGAGATGTT CGATAGCGTT 
TCCATCCTGT TCTCAGACAT CGTTACCTTC ACCGAGATTT GCAGCCGCAT TACTCCGATG 
GAGGTAGTGT CCATGCTGAA CGCTATGTAC TCCATTTTCG ATAAGCTGAC GGAGCGGAAT 
TCAGTGTACA AGGTGGAAAC TATTGGTGAT GCGTATATGG TGGTGGCGGG TGCTCCGGAT 
AAGGATGCCA ATCACGCCGA GCGAGTCTGT GATATGGCCC TGGATATGGT GGATGCGATA 
ACCGATCTAA AGGATCCCTC AACGGGCCAG CACTTGAGGA TACGTGTGGG CGTCCACTCG 
GGCGCAGTGG TGGCGGGCAT CGTGGGCCTT AAGATGCCGC GTTACTGTCT CTTTGGCGAT 
ACGGTCAATA CCGCCTCACG AATGGAGTCC ACCAGCATCG CCATGAAGGT GCACATATCC 
GAGTCTACGA AGGTTCTTAT TGGACCCAAC TACAAGATCA TCGAGCGCGG TGAGATCGAT 
GTAAAGGGCA AGGGCACCAT GGGCACATAC TGGCTGGAGG AACGAGAGAA CCGGCTGCCT 
CTGCAACTAA CCGCTGCTCT GCAAGTTCAT CCGCTCTCAC CTGTCCCACC GACGCCCACA 
CCCAAGACGA AGGCCATCAT GCCTCCTGTA TCCAAGCCGT TGACTCCGAT GATGCCCGTT 
TCAGTTTCCC TGGCAGCTTC CATGCCCACC AGCAACGTTC CTGCTGTGGA TGTGATGGCT 
TCATCATCCA GCATTTCCGG TTTGGCACTA ACAGCCGCTG CAGCGGCCCA CATGTCGCTA 
CATCACCAGG CGGTGGTGGC GGAGGCATTA ACTGGAGCTT CAGTTGAAGT GGCACTACCA 
TCAGTAGCTT CTGGCGCAAC AGGAGCGGCA GCGGGTGGAG GTGCACCGTC GGATGACCGC 
AACAGTCGCA TCTACTCCCC CGTGACCTTC AAGGATGTGG CTCGACGAAG CGTAGCCAAT 
TCTCCGGTAA GGAGCTGTGC TCAACCGGAT CAGGAGAGAC GTCGCGAGTC CCGCTCCAAT 
TCCACGGGCC ACGTGTTTAT GCGAACTCCT TCGGAAATTT TCGGCTCTCT CATCCTGGAC 
ACCGAGGAGT TTCTCGAGGA TCTGCAAATC TCACGTTCCT CCCTGGCAAA CAATAACAAC 
AATCAGAGCC CCTGCGGTTT TAGCCCAACT CCTCCATTCC GGATTGGCAG CGCACCGCCC 
AAGCCGAGAC CTAGTAATCC GGATAAATTT ACACCGGAGG AACTGGCCGC CATGGATCAA 
CTGACGCCTC CGTCAACGGC ACCAGCCAGA GAAACGGCCA GCTGCAGCAG TGCATCATTG 
GATCGCGACA AGGCGACCAA GCTGAAAAAA ATCACTTTCT CCAACAGCAG CAGTCTGGAT 
GCAACCACTC CAACTGCGTT GGCTGCTGTG GTATGTCCAA TGAGAACCAA GTCACCGCCA 
ATGGCAGTGG CTCCAGTGAT GCACATGGTA CAGGCTAGCA CCAGCAAAGG CGACGGCCAA 
CGACCAGGCT CGAAGGATTC AGTATCATCC ATTTCCCTGC ACTCACCTCC TCCGCATCGA 
TCTAACTCGG CACCAGCCAG ACCGCACTCA ATGTCGAAGG CGGCGCGAAA GGCCTTCCTG 
GCCGCCAAAC AGACCAAGGC AATGGAAAAG CTGGACAAGA TGATCGAAGA GGTTCAAGAA 
GTGGAGTCTC AGTCGGTGGC AAAGGCGGCC AACATGCGTC TGGCCCTTTA CGGCCACGAT 
GGAGGTGGTG ATTTGGCTGC CGGTGGGTGT CCACTGTTTC TGCCTCCGCC GCCGCAGCAG 
CAGCAGCAGC AGCAACAGAG CCTGATGCCC AGCTCCATCT CAGACTCCGG CCTCTGCAGT 
CATGGTCATA GTCATGCACC CAGCTGTCAT CATATGGATC CGAAGATGAC CAACAGCCAG 
AGTTTCCAGC ATTCTCCACG TGGCGGAATA ACCCATCAGT GCTGCAGCGG CTTTGGACAT 
GGCAATGGAC GTCATTCGCA CCGGATGCAC TCGAATGCTT GCCGGATTCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gyc88E ( NM_169616.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731974.4 Drosophila melanogaster guanylyl cyclase at 88E, transcript variant F (Gyc88E), mRNA.

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CloneID	OFa04934
Clone ID Related Accession (Same CDS sequence)	NM_169616.4
Accession Version	NM_169616.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 88E, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169616.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACGGAC TGCTGCTGGA GAACCTCTCC GAGTACATCA AGTCCGTTTA CGGCGAGGAG 
AAATGGGAGG ACATCCGGCG ACAGGCTGGC ATCGATTCGC CCTCCTTCAG CGTCCATCAG 
GTCTATCCCG AGAATCTGCT GCAGAAGCTG GCGAAAAAGG CTCAACAGGT TCTGGGCGTT 
TCTGAGCGGG ATTTCATGGA CCAGATGGGC GTCTACTTCG TGGGCTTTGT GGGCCAGTAT 
GGCTACGATC GTGTCCTTTC CGTCCTGGGC CGCCACATGC GCGACTTTCT CAACGGACTG 
GACAACCTGC ATGAGTACCT GAAGTTCTCG TATCCGCGGA TGAGAGCTCC CAGCTTTATC 
TGCGAAAACG AAACGAAGCA GGGTCTGACC CTGCACTATC GTTCCAAGCG TCGAGGATTC 
GTCTACTACA CGATGGGTCA AATCCGAGAG GTGGCACGGT ATTTCTATCA CAAGGAGATG 
CATATTGAGC TCGTTCGGGA AGAGATCCTC TTTGACACGG TCCATGTGAC CTTCCAACTG 
ACCTTCGATA ATCGGGCCTT CACACTTGCA TCCTTGGCCA TGACCCGGGA GGAGAAACAT 
CTGCCCATCA GTGCTCATGT GCTGTTTGAG ATATTTCCCT TTTGCATGGT TTTTGGTGCT 
GATATGGTTG TGCGTAGTAT TGGTAACTCT TTGATGGTGA TTTTACCGGA ACTTCTGGGA 
AAAAAAATTA CGGCCTGGTT CGATCTAGTT CGTCCCTTAA TTGCATTCAA GTTTCAGACT 
ATACTCAATC GTACTAACAA CATTTTCGAG CTGGTCACTG TGGATCCAGT TACGGAGAGA 
TTCGATGTGC AAAACGAGGA CTTACTGCAG CATGAAGATG GCAGCGAACC GGAAAAGTCT 
CTTAGATTAA AAGGTCAAAT GGTTTACATG GAGAACTGGC GCATGATTAT GTTTCTGGGT 
ACTCCCGTAA TGCCGGATCT GACCTCACTA ATAACCACAG GTCTATATAT CAACGATCTG 
TCCATGCACG ACTTCAGCAG AGATCTCATG CTGGCGGGCA CTCAACAATC GGTTGAACTG 
AAGTTGGCAC TGGATCAAGA ACAGCAGAAG TCAAAGAAGC TAGAGGAGTC CATGAGATTG 
TTGGATGAGG AGATGCGTAG AACGGATGAG CTGCTGTATC AGATGATACC CAAACAGGTG 
GCTGATAGGC TCCGACGGGG CGAGAATCCC ATTGATACCT GTGAGATGTT CGATAGCGTT 
TCCATCCTGT TCTCAGACAT CGTTACCTTC ACCGAGATTT GCAGCCGCAT TACTCCGATG 
GAGGTAGTGT CCATGCTGAA CGCTATGTAC TCCATTTTCG ATAAGCTGAC GGAGCGGAAT 
TCAGTGTACA AGGTGGAAAC TATTGGTGAT GCGTATATGG TGGTGGCGGG TGCTCCGGAT 
AAGGATGCCA ATCACGCCGA GCGAGTCTGT GATATGGCCC TGGATATGGT GGATGCGATA 
ACCGATCTAA AGGATCCCTC AACGGGCCAG CACTTGAGGA TACGTGTGGG CGTCCACTCG 
GGCGCAGTGG TGGCGGGCAT CGTGGGCCTT AAGATGCCGC GTTACTGTCT CTTTGGCGAT 
ACGGTCAATA CCGCCTCACG AATGGAGTCC ACCAGCATCG CCATGAAGGT GCACATATCC 
GAGTCTACGA AGGTTCTTAT TGGACCCAAC TACAAGATCA TCGAGCGCGG TGAGATCGAT 
GTAAAGGGCA AGGGCACCAT GGGCACATAC TGGCTGGAGG AACGAGAGAA CCGGCTGCCT 
CTGCAACTAA CCGCTGCTCT GCAAGTTCAT CCGCTCTCAC CTGTCCCACC GACGCCCACA 
CCCAAGACGA AGGCCATCAT GCCTCCTGTA TCCAAGCCGT TGACTCCGAT GATGCCCGTT 
TCAGTTTCCC TGGCAGCTTC CATGCCCACC AGCAACGTTC CTGCTGTGGA TGTGATGGCT 
TCATCATCCA GCATTTCCGG TTTGGCACTA ACAGCCGCTG CAGCGGCCCA CATGTCGCTA 
CATCACCAGG CGGTGGTGGC GGAGGCATTA ACTGGAGCTT CAGTTGAAGT GGCACTACCA 
TCAGTAGCTT CTGGCGCAAC AGGAGCGGCA GCGGGTGGAG GTGCACCGTC GGATGACCGC 
AACAGTCGCA TCTACTCCCC CGTGACCTTC AAGGATGTGG CTCGACGAAG CGTAGCCAAT 
TCTCCGGTAA GGAGCTGTGC TCAACCGGAT CAGGAGAGAC GTCGCGAGTC CCGCTCCAAT 
TCCACGGGCC ACGTGTTTAT GCGAACTCCT TCGGAAATTT TCGGCTCTCT CATCCTGGAC 
ACCGAGGAGT TTCTCGAGGA TCTGCAAATC TCACGTTCCT CCCTGGCAAA CAATAACAAC 
AATCAGAGCC CCTGCGGTTT TAGCCCAACT CCTCCATTCC GGATTGGCAG CGCACCGCCC 
AAGCCGAGAC CTAGTAATCC GGATAAATTT ACACCGGAGG AACTGGCCGC CATGGATCAA 
CTGACGCCTC CGTCAACGGC ACCAGCCAGA GAAACGGCCA GCTGCAGCAG TGCATCATTG 
GATCGCGACA AGGCGACCAA GCTGAACAGC AGTCTGGATG CAACCACTCC AACTGCGTTG 
GCTGCTGTGG TATGTCCAAT GAGAACCAAG TCACCGCCAA TGGCAGTGGC TCCAGTGATG 
CACATGGTAC AGGCTAGCAC CAGCAAAGGC GACGGCCAAC GACCAGGCTC GAAGGATTCA 
GTATCATCCA TTTCCCTGCA CTCACCTCCT CCGCATCGAT CTAACTCGGC ACCAGCCAGA 
CCGCACTCAA TGTCGAAGGC GGCGCGAAAG GCCTTCCTGG CCGCCAAACA GACCAAGGCA 
ATGGAAAAGC TGGACAAGAT GATCGAAGAG GTTCAAGAAG TGGAGTCTCA GTCGGTGGCA 
AAGGCGGCCA ACATGCGTCT GGCCCTTTAC GGCCACGATG GAGGTGGTGA TTTGGCTGCC 
GGTGGGTGTC CACTGTTTCT GCCTCCGCCG CCGCAGCAGC AGCAGCAGCA GCAACAGAGC 
CTGATGCCCA GCTCCATCTC AGACTCCGGC CTCTGCAGTC ATGGTCATAG TCATGCACCC 
AGCTGTCATC ATATGGATCC GAAGATGACC AACAGCCAGA GTTTCCAGCA TTCTCCACGT 
GGCGGAATAA CCCATCAGTG CTGCAGCGGC TTTGGACATG GCAATGGACG TCATTCGCAC 
CGGATGCACT CGAATGCTTG CCGGATTCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731974.4
CDS	361..3633
Translation	MYGLLLENLSEYIKSVYGEEKWEDIRRQAGIDSPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQYGYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEMHIELVREEILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLPISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLGKKITAWFDLVRPLIAFKFQTILNRTNNIFELVTVDPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLGTPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKKLEESMRLLDEEMRRTDELLYQMIPKQVADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPDKDANHAERVCDMALDMVDAITDLKDPSTGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHISESTKVLIGPNYKIIERGEIDVKGKGTMGTYWLEERENRLPLQLTAALQVHPLSPVPPTPTPKTKAIMPPVSKPLTPMMPVSVSLAASMPTSNVPAVDVMASSSSISGLALTAAAAAHMSLHHQAVVAEALTGASVEVALPSVASGATGAAAGGGAPSDDRNSRIYSPVTFKDVARRSVANSPVRSCAQPDQERRRESRSNSTGHVFMRTPSEIFGSLILDTEEFLEDLQISRSSLANNNNNQSPCGFSPTPPFRIGSAPPKPRPSNPDKFTPEELAAMDQLTPPSTAPARETASCSSASLDRDKATKLNSSLDATTPTALAAVVCPMRTKSPPMAVAPVMHMVQASTSKGDGQRPGSKDSVSSISLHSPPPHRSNSAPARPHSMSKAARKAFLAAKQTKAMEKLDKMIEEVQEVESQSVAKAANMRLALYGHDGGGDLAAGGCPLFLPPPPQQQQQQQQSLMPSSISDSGLCSHGHSHAPSCHHMDPKMTNSQSFQHSPRGGITHQCCSGFGHGNGRHSHRMHSNACRIL

Target ORF information:

RefSeq Version	NM_169616.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 88E, transcript variant F (Gyc88E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169616.4

ORF Insert Sequence:

ATGTACGGAC TGCTGCTGGA GAACCTCTCC GAGTACATCA AGTCCGTTTA CGGCGAGGAG 
AAATGGGAGG ACATCCGGCG ACAGGCTGGC ATCGATTCGC CCTCCTTCAG CGTCCATCAG 
GTCTATCCCG AGAATCTGCT GCAGAAGCTG GCGAAAAAGG CTCAACAGGT TCTGGGCGTT 
TCTGAGCGGG ATTTCATGGA CCAGATGGGC GTCTACTTCG TGGGCTTTGT GGGCCAGTAT 
GGCTACGATC GTGTCCTTTC CGTCCTGGGC CGCCACATGC GCGACTTTCT CAACGGACTG 
GACAACCTGC ATGAGTACCT GAAGTTCTCG TATCCGCGGA TGAGAGCTCC CAGCTTTATC 
TGCGAAAACG AAACGAAGCA GGGTCTGACC CTGCACTATC GTTCCAAGCG TCGAGGATTC 
GTCTACTACA CGATGGGTCA AATCCGAGAG GTGGCACGGT ATTTCTATCA CAAGGAGATG 
CATATTGAGC TCGTTCGGGA AGAGATCCTC TTTGACACGG TCCATGTGAC CTTCCAACTG 
ACCTTCGATA ATCGGGCCTT CACACTTGCA TCCTTGGCCA TGACCCGGGA GGAGAAACAT 
CTGCCCATCA GTGCTCATGT GCTGTTTGAG ATATTTCCCT TTTGCATGGT TTTTGGTGCT 
GATATGGTTG TGCGTAGTAT TGGTAACTCT TTGATGGTGA TTTTACCGGA ACTTCTGGGA 
AAAAAAATTA CGGCCTGGTT CGATCTAGTT CGTCCCTTAA TTGCATTCAA GTTTCAGACT 
ATACTCAATC GTACTAACAA CATTTTCGAG CTGGTCACTG TGGATCCAGT TACGGAGAGA 
TTCGATGTGC AAAACGAGGA CTTACTGCAG CATGAAGATG GCAGCGAACC GGAAAAGTCT 
CTTAGATTAA AAGGTCAAAT GGTTTACATG GAGAACTGGC GCATGATTAT GTTTCTGGGT 
ACTCCCGTAA TGCCGGATCT GACCTCACTA ATAACCACAG GTCTATATAT CAACGATCTG 
TCCATGCACG ACTTCAGCAG AGATCTCATG CTGGCGGGCA CTCAACAATC GGTTGAACTG 
AAGTTGGCAC TGGATCAAGA ACAGCAGAAG TCAAAGAAGC TAGAGGAGTC CATGAGATTG 
TTGGATGAGG AGATGCGTAG AACGGATGAG CTGCTGTATC AGATGATACC CAAACAGGTG 
GCTGATAGGC TCCGACGGGG CGAGAATCCC ATTGATACCT GTGAGATGTT CGATAGCGTT 
TCCATCCTGT TCTCAGACAT CGTTACCTTC ACCGAGATTT GCAGCCGCAT TACTCCGATG 
GAGGTAGTGT CCATGCTGAA CGCTATGTAC TCCATTTTCG ATAAGCTGAC GGAGCGGAAT 
TCAGTGTACA AGGTGGAAAC TATTGGTGAT GCGTATATGG TGGTGGCGGG TGCTCCGGAT 
AAGGATGCCA ATCACGCCGA GCGAGTCTGT GATATGGCCC TGGATATGGT GGATGCGATA 
ACCGATCTAA AGGATCCCTC AACGGGCCAG CACTTGAGGA TACGTGTGGG CGTCCACTCG 
GGCGCAGTGG TGGCGGGCAT CGTGGGCCTT AAGATGCCGC GTTACTGTCT CTTTGGCGAT 
ACGGTCAATA CCGCCTCACG AATGGAGTCC ACCAGCATCG CCATGAAGGT GCACATATCC 
GAGTCTACGA AGGTTCTTAT TGGACCCAAC TACAAGATCA TCGAGCGCGG TGAGATCGAT 
GTAAAGGGCA AGGGCACCAT GGGCACATAC TGGCTGGAGG AACGAGAGAA CCGGCTGCCT 
CTGCAACTAA CCGCTGCTCT GCAAGTTCAT CCGCTCTCAC CTGTCCCACC GACGCCCACA 
CCCAAGACGA AGGCCATCAT GCCTCCTGTA TCCAAGCCGT TGACTCCGAT GATGCCCGTT 
TCAGTTTCCC TGGCAGCTTC CATGCCCACC AGCAACGTTC CTGCTGTGGA TGTGATGGCT 
TCATCATCCA GCATTTCCGG TTTGGCACTA ACAGCCGCTG CAGCGGCCCA CATGTCGCTA 
CATCACCAGG CGGTGGTGGC GGAGGCATTA ACTGGAGCTT CAGTTGAAGT GGCACTACCA 
TCAGTAGCTT CTGGCGCAAC AGGAGCGGCA GCGGGTGGAG GTGCACCGTC GGATGACCGC 
AACAGTCGCA TCTACTCCCC CGTGACCTTC AAGGATGTGG CTCGACGAAG CGTAGCCAAT 
TCTCCGGTAA GGAGCTGTGC TCAACCGGAT CAGGAGAGAC GTCGCGAGTC CCGCTCCAAT 
TCCACGGGCC ACGTGTTTAT GCGAACTCCT TCGGAAATTT TCGGCTCTCT CATCCTGGAC 
ACCGAGGAGT TTCTCGAGGA TCTGCAAATC TCACGTTCCT CCCTGGCAAA CAATAACAAC 
AATCAGAGCC CCTGCGGTTT TAGCCCAACT CCTCCATTCC GGATTGGCAG CGCACCGCCC 
AAGCCGAGAC CTAGTAATCC GGATAAATTT ACACCGGAGG AACTGGCCGC CATGGATCAA 
CTGACGCCTC CGTCAACGGC ACCAGCCAGA GAAACGGCCA GCTGCAGCAG TGCATCATTG 
GATCGCGACA AGGCGACCAA GCTGAACAGC AGTCTGGATG CAACCACTCC AACTGCGTTG 
GCTGCTGTGG TATGTCCAAT GAGAACCAAG TCACCGCCAA TGGCAGTGGC TCCAGTGATG 
CACATGGTAC AGGCTAGCAC CAGCAAAGGC GACGGCCAAC GACCAGGCTC GAAGGATTCA 
GTATCATCCA TTTCCCTGCA CTCACCTCCT CCGCATCGAT CTAACTCGGC ACCAGCCAGA 
CCGCACTCAA TGTCGAAGGC GGCGCGAAAG GCCTTCCTGG CCGCCAAACA GACCAAGGCA 
ATGGAAAAGC TGGACAAGAT GATCGAAGAG GTTCAAGAAG TGGAGTCTCA GTCGGTGGCA 
AAGGCGGCCA ACATGCGTCT GGCCCTTTAC GGCCACGATG GAGGTGGTGA TTTGGCTGCC 
GGTGGGTGTC CACTGTTTCT GCCTCCGCCG CCGCAGCAGC AGCAGCAGCA GCAACAGAGC 
CTGATGCCCA GCTCCATCTC AGACTCCGGC CTCTGCAGTC ATGGTCATAG TCATGCACCC 
AGCTGTCATC ATATGGATCC GAAGATGACC AACAGCCAGA GTTTCCAGCA TTCTCCACGT 
GGCGGAATAA CCCATCAGTG CTGCAGCGGC TTTGGACATG GCAATGGACG TCATTCGCAC 
CGGATGCACT CGAATGCTTG CCGGATTCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gyc88E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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