eff cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eff
Entrez Gene ID	41785
Full Name	effete
Synonyms	3527,6535,CG7425,Dmel\CG7425,E(Pc)88D,EFF,Eff,SS3-3,UBC4,UBCD-1,UBCD1,Ubc-D1,UbcD,UbcD1,UbcH5,anon-WO0118547.343,fs(3)01295,l(3)01462,l(3)j2C8,l(3)s1782,mei68,ms(3)01295,ms(3)1,ms(3)3,ms(3)88D,ms(3)neo2,ms(3)ry1,ms(3)ry3,ubcD1
General protein information	Preferred Names effete Names CG7425 gene product from transcript CG7425-RC CG7425-PA CG7425-PB CG7425-PC eff-PA eff-PB eff-PC effette meiotic 68 suppressor of GMR-sina 3-3 ubiquitin conjugating enzyme 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88D2-88D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_169601.2	NP_731941.1	effete, isoform A
NM_001275649.1	NP_001262578.1	effete, isoform B
NM_001275650.1	NP_001262579.1	effete, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE2D2	NP_003330.1
Pan troglodytes (chimpanzee)	UBE2D2	XP_517968.1
Rattus norvegicus (Norway rat)	Ube2d2	NP_001032369.1
Kluyveromyces lactis	KLLA0E12563g	XP_454516.1
Magnaporthe oryzae (rice blast fungus)	MGG_06562	XP_003716978.1
Neurospora crassa	NCU02289	XP_959933.2
	LOC694667	XP_002804584.1
Canis lupus familiaris (dog)	UBE2D2	XP_855983.1
Bos taurus (cattle)	UBE2D2	NP_001039961.1
Mus musculus (house mouse)	Ube2d2a	NP_064296.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000145	XP_310998.2
Caenorhabditis elegans (roundworm)	let-70	NP_502065.1
Oryza sativa (rice)	Os09g0293400	NP_001062791.1
Xenopus tropicalis (tropical clawed frog)	ube2d3	NP_001016064.1
Gallus gallus (chicken)	UBE2D2	XP_414470.4
Danio rerio (zebrafish)	zgc:77149	NP_957253.1
Saccharomyces cerevisiae (baker's yeast)	UBC4	NP_009638.1
Eremothecium gossypii	AGOS_AER173C	NP_985031.1
Schizosaccharomyces pombe (fission yeast)	ubc4	NP_595283.1
Drosophila melanogaster (fruit fly)	eff	NP_001262578.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

eff regulates both telomere position effect and position effect variegation.
Title: Effete, a Drosophila chromatin-associated ubiquitin-conjugating enzyme that affects telomeric and heterochromatic position effect variegation.

Effete/APC-mediated degradation of Cyclin A is essential for the maintenance of germline stem cells
Title: Effete-mediated degradation of Cyclin A is essential for the maintenance of germline stem cells in Drosophila.

the UbcD1 substrate(s) binds chromosomal termini in a sequence-independent manner
Title: The role of HeT-A and TART retrotransposons in Drosophila telomere capping.

Regulation of Drosophila IAP1 degradation and apoptosis by reaper and ubcD1.
Title: Regulation of Drosophila IAP1 degradation and apoptosis by reaper and ubcD1.

The following eff gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eff cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04897	NM_001275650.1 Latest version!	Drosophila melanogaster effete (eff), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa04897	NM_001275649.1 Latest version!	Drosophila melanogaster effete (eff), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa04897	NM_169601.2 Latest version!	Drosophila melanogaster effete (eff), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eff ( NM_169601.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731941.1 Drosophila melanogaster effete (eff), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04897
Clone ID Related Accession (Same CDS sequence)	NM_169601.2 , NM_001275649.1 , NM_001275650.1
Accession Version	NM_169601.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	effete, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169601.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTTAA AAAGAATCAA TAAGGAACTG CAAGATCTGG GCAGAGATCC ACCTGCACAA 
TGTTCAGCCG GTCCAGTTGG AGATGATTTA TTTCACTGGC AAGCTACAAT AATGGGCCCG 
CCGGACAGCC CTTATCAAGG AGGTGTATTC TTCTTAACTA TACATTTTCC AACAGACTAT 
CCCTTTAAAC CACCCAAAGT GGCTTTTACA ACGCGCATAT ACCATCCAAA CATCAACAGC 
AATGGATCGA TTTGTCTCGA TATATTAAGA TCTCAGTGGT CGCCAGCATT AACTATTTCA 
AAAGTTTTAT TATCAATTTG CTCTCTACTC TGTGATCCCA ATCCAGACGA TCCTCTTGTT 
CCAGAGATTG CCAGAATATA TAAAACCGAT CGGGAAAAAT ACAATGAGCT GGCACGAGAG 
TGGACTAGAA AGTATGCTAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731941.1
CDS	851..1294
Translation	MALKRINKELQDLGRDPPAQCSAGPVGDDLFHWQATIMGPPDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNELAREWTRKYAM

Target ORF information:

RefSeq Version	NM_169601.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster effete (eff), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169601.2

ORF Insert Sequence:

ATGGCGTTAA AAAGAATCAA TAAGGAACTG CAAGATCTGG GCAGAGATCC ACCTGCACAA 
TGTTCAGCCG GTCCAGTTGG AGATGATTTA TTTCACTGGC AAGCTACAAT AATGGGCCCG 
CCGGACAGCC CTTATCAAGG AGGTGTATTC TTCTTAACTA TACATTTTCC AACAGACTAT 
CCCTTTAAAC CACCCAAAGT GGCTTTTACA ACGCGCATAT ACCATCCAAA CATCAACAGC 
AATGGATCGA TTTGTCTCGA TATATTAAGA TCTCAGTGGT CGCCAGCATT AACTATTTCA 
AAAGTTTTAT TATCAATTTG CTCTCTACTC TGTGATCCCA ATCCAGACGA TCCTCTTGTT 
CCAGAGATTG CCAGAATATA TAAAACCGAT CGGGAAAAAT ACAATGAGCT GGCACGAGAG 
TGGACTAGAA AGTATGCTAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eff ( NM_001275649.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262578.1 Drosophila melanogaster effete (eff), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04897
Clone ID Related Accession (Same CDS sequence)	NM_169601.2 , NM_001275649.1 , NM_001275650.1
Accession Version	NM_001275649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	effete, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTTAA AAAGAATCAA TAAGGAACTG CAAGATCTGG GCAGAGATCC ACCTGCACAA 
TGTTCAGCCG GTCCAGTTGG AGATGATTTA TTTCACTGGC AAGCTACAAT AATGGGCCCG 
CCGGACAGCC CTTATCAAGG AGGTGTATTC TTCTTAACTA TACATTTTCC AACAGACTAT 
CCCTTTAAAC CACCCAAAGT GGCTTTTACA ACGCGCATAT ACCATCCAAA CATCAACAGC 
AATGGATCGA TTTGTCTCGA TATATTAAGA TCTCAGTGGT CGCCAGCATT AACTATTTCA 
AAAGTTTTAT TATCAATTTG CTCTCTACTC TGTGATCCCA ATCCAGACGA TCCTCTTGTT 
CCAGAGATTG CCAGAATATA TAAAACCGAT CGGGAAAAAT ACAATGAGCT GGCACGAGAG 
TGGACTAGAA AGTATGCTAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262578.1
CDS	389..832
Translation	MALKRINKELQDLGRDPPAQCSAGPVGDDLFHWQATIMGPPDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNELAREWTRKYAM

Target ORF information:

RefSeq Version	NM_001275649.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster effete (eff), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275649.1

ORF Insert Sequence:

ATGGCGTTAA AAAGAATCAA TAAGGAACTG CAAGATCTGG GCAGAGATCC ACCTGCACAA 
TGTTCAGCCG GTCCAGTTGG AGATGATTTA TTTCACTGGC AAGCTACAAT AATGGGCCCG 
CCGGACAGCC CTTATCAAGG AGGTGTATTC TTCTTAACTA TACATTTTCC AACAGACTAT 
CCCTTTAAAC CACCCAAAGT GGCTTTTACA ACGCGCATAT ACCATCCAAA CATCAACAGC 
AATGGATCGA TTTGTCTCGA TATATTAAGA TCTCAGTGGT CGCCAGCATT AACTATTTCA 
AAAGTTTTAT TATCAATTTG CTCTCTACTC TGTGATCCCA ATCCAGACGA TCCTCTTGTT 
CCAGAGATTG CCAGAATATA TAAAACCGAT CGGGAAAAAT ACAATGAGCT GGCACGAGAG 
TGGACTAGAA AGTATGCTAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eff ( NM_001275650.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262579.1 Drosophila melanogaster effete (eff), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04897
Clone ID Related Accession (Same CDS sequence)	NM_169601.2 , NM_001275649.1 , NM_001275650.1
Accession Version	NM_001275650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	effete, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTTAA AAAGAATCAA TAAGGAACTG CAAGATCTGG GCAGAGATCC ACCTGCACAA 
TGTTCAGCCG GTCCAGTTGG AGATGATTTA TTTCACTGGC AAGCTACAAT AATGGGCCCG 
CCGGACAGCC CTTATCAAGG AGGTGTATTC TTCTTAACTA TACATTTTCC AACAGACTAT 
CCCTTTAAAC CACCCAAAGT GGCTTTTACA ACGCGCATAT ACCATCCAAA CATCAACAGC 
AATGGATCGA TTTGTCTCGA TATATTAAGA TCTCAGTGGT CGCCAGCATT AACTATTTCA 
AAAGTTTTAT TATCAATTTG CTCTCTACTC TGTGATCCCA ATCCAGACGA TCCTCTTGTT 
CCAGAGATTG CCAGAATATA TAAAACCGAT CGGGAAAAAT ACAATGAGCT GGCACGAGAG 
TGGACTAGAA AGTATGCTAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262579.1
CDS	389..832
Translation	MALKRINKELQDLGRDPPAQCSAGPVGDDLFHWQATIMGPPDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNELAREWTRKYAM

Target ORF information:

RefSeq Version	NM_001275650.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster effete (eff), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275650.1

ORF Insert Sequence:

ATGGCGTTAA AAAGAATCAA TAAGGAACTG CAAGATCTGG GCAGAGATCC ACCTGCACAA 
TGTTCAGCCG GTCCAGTTGG AGATGATTTA TTTCACTGGC AAGCTACAAT AATGGGCCCG 
CCGGACAGCC CTTATCAAGG AGGTGTATTC TTCTTAACTA TACATTTTCC AACAGACTAT 
CCCTTTAAAC CACCCAAAGT GGCTTTTACA ACGCGCATAT ACCATCCAAA CATCAACAGC 
AATGGATCGA TTTGTCTCGA TATATTAAGA TCTCAGTGGT CGCCAGCATT AACTATTTCA 
AAAGTTTTAT TATCAATTTG CTCTCTACTC TGTGATCCCA ATCCAGACGA TCCTCTTGTT 
CCAGAGATTG CCAGAATATA TAAAACCGAT CGGGAAAAAT ACAATGAGCT GGCACGAGAG 
TGGACTAGAA AGTATGCTAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eff cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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