l(3)L1231 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	l(3)L1231
Entrez Gene ID	41776
Full Name	lethal (3) L1231
Synonyms	BEST:LD14744,CG7832,Dmel\CG7832,LD14744
General protein information	Preferred Names lethal (3) L1231 Names CG7832 gene product from transcript CG7832-RD CG7832-PA CG7832-PC CG7832-PD l(3)L1231-PA l(3)L1231-PC l(3)L1231-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88C10-88C10

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300399.1	NP_001287328.1	lethal (3) L1231, isoform D
NM_001260175.2	NP_001247104.1	lethal (3) L1231, isoform C
NM_142122.4	NP_650379.1	lethal (3) L1231, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following l(3)L1231 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(3)L1231 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04889	NM_001300399.1 Latest version!	Drosophila melanogaster lethal (3) L1231 (l(3)L1231), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa04889	NM_001260175.2 Latest version!	Drosophila melanogaster lethal (3) L1231 (l(3)L1231), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa04889	NM_142122.4 Latest version!	Drosophila melanogaster lethal (3) L1231 (l(3)L1231), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

l(3)L1231 ( NM_001300399.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287328.1 Drosophila melanogaster lethal (3) L1231 (l(3)L1231), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa04889
Clone ID Related Accession (Same CDS sequence)	NM_001300399.1 , NM_001260175.2 , NM_142122.4
Accession Version	NM_001300399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (3) L1231, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATGTC AGCAAGCCTC CAGATTCCAT TCAGCCACCG CTACAGCCAC ATTAGCCAAG 
AGCACAGCCA CCAGAAGGAT TGCCACAGCA GCAGCAGCCG CAACAGCAAC AGCGATAGCC 
GCAGCAACAG CCGCAGCAGC AGCAGCAACG CTGACGCCCG TGGAGAGCAT TGCCGGCAAG 
ACGACGTCCG AGGACTCGGA TCCCTATGCC TTCACCGAGA CTGTGGCCGT CACACCACCC 
ATTCTATTCA ATGCACAGAA ATCGAGAGCC CGCCTAACCG ACAGCAATAG AGGCAGCAAC 
AAGAGGCAGA CGGCAGCAAC AGCTGCGGCC AACAGAAAGG CGAACCTGGT GGCCCAACTG 
AGTGTCACAG AGGCAGCAAA GGCGCAGGCG TCTTTGGCAA GCAACAACAC AACGAATTTC 
CATCATGTCA CGCAATCTCA GAGACAGTCG ACGGCGCTGC AGTTGCAATT GCCACTGCAA 
TCCCAGTCAC AGTCGCAGGC CTCGCCGAAG CGGGCCACCA ACGTGTGCAT AGTCCGCCCG 
CAGCAACAGC AGCTGGAGAA GATAGCCACC TCGGAGTCCT GCCAGTCGCC GGCAGCACCA 
CCACCGCTTT ACGCCCACAC TCCATCGCTG TGGCAGACGC CGCTGCTCAT AGACAATGGG 
CAAAAGCAAC AGCTCCTCCA GCAGCAGCAT CAGCAACCGC AACAGCAACA GTCCGTTGCT 
ATTGCGTTGG TCAGTCCGCC CACATCGCCC GCCTCATTAC CTTCGCCCAC TCTGCCGCCT 
GCCACCGCTG CAGTGACCGC CATGGTGGCA CCGATTTCCG TATCGCCCAA GGGTGGATTA 
CCTTTGCCGC CATCGAAGTT CCATCACACC ACACTGGCGC AACATCTGCA GAAGGTGGAG 
TGCTTGAAAA AAAAGAAATC CTTGCCACTG GCCTGCCAAA ACAACAACAA TAACAGCAAT 
TTGCCGAATA ACAACAATGT GGAGTCGCTT AAGAAACCGG TGGTGCAGGG AACGAGCTAC 
AATCAGACTC ATCCGCCACC GCTGATGGTT TTCAATACGG GAACAGTTGC AGTTCCCGCG 
CAGAGTCCGC AGACTGCTGC TCCACAGAAA CATTCCACCG GCAACAGCGT AGATGACAGC 
GATCTCAACG AGATACCCGT CAATGTTATC TTCAGAAAGC CGCAAGAGGC AGGCGGAGCG 
GCGAAAACAG GTGGACCGGG AGGATTAAGT GCACCTGTTT CGGGAACGCC GCAAACTCGT 
CCAGCTGAAG TGAAAATGGT GACTCCTCTC ACGCCGCCCA CTCCACCAGA GATGAGCGCA 
CCGCCCCTGC TAGCGCAAAT GCAACCCCCG CAGATACCCA CGTCTTGTGT TCCAGCTTTA 
GCTCCCAGCT TCAAAGTGTC GTCACCAGCA GTTCTCAGCC CGAAGGTGAT CTCACCAGCT 
CCTGCAAGCC CAAAGCTCTT GTGTCCGACA GCACCCGCTT CAACGAACTC ACTGCAAATT 
GCGCCAAAAG CTGTTCCAGC CACTACAACC TCATCTGCAC CAGCATTAGC CACGAAATCA 
GAACAAATGT CTTCCAAAGT GGCCAATTTA AACGCCTTCA ACCGTCAAGC ACCCATAGCT 
TCAAAGGGCG TTAGAAATGG CATCACTAAC AACAATAACA ACAGAAACAG CAATGTCGTT 
GCGAAGAAAT CGTCACCAAC TTCGATGCCG CCTCCAAAGG ATCCGATTGC ACCGATTGCA 
GCAAACGAGT TGACGGATTC CGAACATCGT CAGCGACGCC GCAAGCCCGC CTCCGCCTGC 
AGACGCAGCT TGGACACTCT GAGTGAGAAC GAGTCCTTTT CAGTGGATTC GCCCTACTGC 
CTACAACAGC ATTGGCTGCA CTCCGGCTTT AATAATAAGT CCCACGACGC GCCACTATCG 
CAAAGTAACC GGCGTGAGGA TCGAATTGCT GTTCGTAAAG GAGCCCTGAG GCGACAAGCG 
CTGCAGCTAC TGTCTACGCG CTCCCTGCAG GAGCTTCCCA TGCGAGCGGC CAAACAGCGG 
CTGCAGTGCG TCCAGAACAT GCTGATCAAG TACCAAGATC ACGCTGGCCA GCAGCAGGGA 
ATTGGAATTG GCAACCACTG TCTGGTGGCC AGCTGCAAGC AGCCCACGCT TAGTATGGCA 
GCGCACTGCG AGCGTCACAT CGTGAACAAC AGCACCCAGC AGCTTTTCCA GCCTTGCGTC 
GCCTGGCGGA TGGATGGAAC CGCTTGCCAG GCTCCTGTCT TCGATGTGCT GCACACATTG 
GCGCTCTGCA AGGTGCACAG CCACCTGCGT TCTGGTATGG ATGGAGCCCG TCCAGCATCG 
AAACAGCCGC CCGTTAGTTT ACCAGTCGCT GGAGTGGCGA CTCTTTATGT GCCTGTGAAG 
CAGCAGCGAA AGCGCAAGGC TAATACGAAT GCCGTGGCTC GTCCTCAGAA ACGTGGTAGG 
AAGCCGGCGA ATGAACCGAT TGCTAACCAG ATCAGCAGCC AGATAAACAA ACTGATACCT 
GGGGCAGGAA TGCAGCGTAA AAGCAGCACC ACATCGCTTG AGTCCATTGC CAGTAATTCG 
CAATCGTCGG CCACCTCACA CTCACAGCCG CCTTACAAGC CCGGAAATGT GTTGGCCGCT 
GTACCTGCAG CACAGAACTT GTCCCAACGG TCCATTCCTC CAGCGCTGGC TCCGCTCAGC 
AGTGATTTTC AACCCAACCA TCAGCAGCAG GAACCGCTTT TGGTTCCCAA GTTGGAGGTG 
GATTCATTGT TTAAATTTGA TGCCGATCAA CAGCAAAATC AGCAGCAGCA ATCGACTTTG 
GACCCCAGCT TACTTTCTTT GGACATTGCG AATATCAAGG CCGAGGAGAT TAGCCAAATT 
GTAGCACAAT TGGCGGCGGC CGGTGGTGAT TTACAAAATC CCAACAATAA TAATAACAAC 
AACATAGGAA TCCATAATAA CAATAGCGTG CACTTCAACA ACAACAACAA CAATAGTATG 
AACTACAGCA ACAATAACAA TAACAGTTTC CCCTCATTCA ACACGGCCTT TGGCAACGCC 
ATGGGCCAGC CAAGCAACAC AATTACGCAC AATGGCCACG CTGTTCCGGC AGTGCTGGCC 
AATACGGCCG ATCTTCTTGG CCAGGACATG TTTGGTATTT GCGAGAACAG CTCGGCATAC 
GCCAGTTCCG AGGATACCGG ATTGGGCGGT CTCAGCGAGT CGGAGCTGAT AGGCACTAAC 
GATGCTGATG ATATAGCATT GAATGGCGCC CACTTGCTGG AGGAGCACGA TCTGGTAAAT 
GTGTTCGACA CGCTGTCAGA CGATGCCTTC AACGAGCTGT TCCAATCCGT GCAACAAGCC 
GAGTGCGAGG CTATGGACCG GGCTTTGGAC CGGGCCTTAC AGCAGACAAT GGGCGGCTCG 
GCGGACAGCG CCTTTCTTAA CGATTTCCTG GACGTCGGCG ACGATCTGCT GGCCGATGCT 
GTGATGCACT CACCAAACAC GTCCGGCATC GATGCTCCTC CCCTCTTTGG GGACAGCAGC 
AGCGGCGGTG GCAATAGCAG CAGCAACGGC GCCTCCGACA TCCGGGGCTT GGTGCAGACC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287328.1
CDS	142..3744
Translation	MSCQQASRFHSATATATLAKSTATRRIATAAAAATATAIAAATAAAAAATLTPVESIAGKTTSEDSDPYAFTETVAVTPPILFNAQKSRARLTDSNRGSNKRQTAATAAANRKANLVAQLSVTEAAKAQASLASNNTTNFHHVTQSQRQSTALQLQLPLQSQSQSQASPKRATNVCIVRPQQQQLEKIATSESCQSPAAPPPLYAHTPSLWQTPLLIDNGQKQQLLQQQHQQPQQQQSVAIALVSPPTSPASLPSPTLPPATAAVTAMVAPISVSPKGGLPLPPSKFHHTTLAQHLQKVECLKKKKSLPLACQNNNNNSNLPNNNNVESLKKPVVQGTSYNQTHPPPLMVFNTGTVAVPAQSPQTAAPQKHSTGNSVDDSDLNEIPVNVIFRKPQEAGGAAKTGGPGGLSAPVSGTPQTRPAEVKMVTPLTPPTPPEMSAPPLLAQMQPPQIPTSCVPALAPSFKVSSPAVLSPKVISPAPASPKLLCPTAPASTNSLQIAPKAVPATTTSSAPALATKSEQMSSKVANLNAFNRQAPIASKGVRNGITNNNNNRNSNVVAKKSSPTSMPPPKDPIAPIAANELTDSEHRQRRRKPASACRRSLDTLSENESFSVDSPYCLQQHWLHSGFNNKSHDAPLSQSNRREDRIAVRKGALRRQALQLLSTRSLQELPMRAAKQRLQCVQNMLIKYQDHAGQQQGIGIGNHCLVASCKQPTLSMAAHCERHIVNNSTQQLFQPCVAWRMDGTACQAPVFDVLHTLALCKVHSHLRSGMDGARPASKQPPVSLPVAGVATLYVPVKQQRKRKANTNAVARPQKRGRKPANEPIANQISSQINKLIPGAGMQRKSSTTSLESIASNSQSSATSHSQPPYKPGNVLAAVPAAQNLSQRSIPPALAPLSSDFQPNHQQQEPLLVPKLEVDSLFKFDADQQQNQQQQSTLDPSLLSLDIANIKAEEISQIVAQLAAAGGDLQNPNNNNNNNIGIHNNNSVHFNNNNNNSMNYSNNNNNSFPSFNTAFGNAMGQPSNTITHNGHAVPAVLANTADLLGQDMFGICENSSAYASSEDTGLGGLSESELIGTNDADDIALNGAHLLEEHDLVNVFDTLSDDAFNELFQSVQQAECEAMDRALDRALQQTMGGSADSAFLNDFLDVGDDLLADAVMHSPNTSGIDAPPLFGDSSSGGGNSSSNGASDIRGLVQT

Target ORF information:

RefSeq Version	NM_001300399.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (3) L1231 (l(3)L1231), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300399.1

ORF Insert Sequence:

ATGTCATGTC AGCAAGCCTC CAGATTCCAT TCAGCCACCG CTACAGCCAC ATTAGCCAAG 
AGCACAGCCA CCAGAAGGAT TGCCACAGCA GCAGCAGCCG CAACAGCAAC AGCGATAGCC 
GCAGCAACAG CCGCAGCAGC AGCAGCAACG CTGACGCCCG TGGAGAGCAT TGCCGGCAAG 
ACGACGTCCG AGGACTCGGA TCCCTATGCC TTCACCGAGA CTGTGGCCGT CACACCACCC 
ATTCTATTCA ATGCACAGAA ATCGAGAGCC CGCCTAACCG ACAGCAATAG AGGCAGCAAC 
AAGAGGCAGA CGGCAGCAAC AGCTGCGGCC AACAGAAAGG CGAACCTGGT GGCCCAACTG 
AGTGTCACAG AGGCAGCAAA GGCGCAGGCG TCTTTGGCAA GCAACAACAC AACGAATTTC 
CATCATGTCA CGCAATCTCA GAGACAGTCG ACGGCGCTGC AGTTGCAATT GCCACTGCAA 
TCCCAGTCAC AGTCGCAGGC CTCGCCGAAG CGGGCCACCA ACGTGTGCAT AGTCCGCCCG 
CAGCAACAGC AGCTGGAGAA GATAGCCACC TCGGAGTCCT GCCAGTCGCC GGCAGCACCA 
CCACCGCTTT ACGCCCACAC TCCATCGCTG TGGCAGACGC CGCTGCTCAT AGACAATGGG 
CAAAAGCAAC AGCTCCTCCA GCAGCAGCAT CAGCAACCGC AACAGCAACA GTCCGTTGCT 
ATTGCGTTGG TCAGTCCGCC CACATCGCCC GCCTCATTAC CTTCGCCCAC TCTGCCGCCT 
GCCACCGCTG CAGTGACCGC CATGGTGGCA CCGATTTCCG TATCGCCCAA GGGTGGATTA 
CCTTTGCCGC CATCGAAGTT CCATCACACC ACACTGGCGC AACATCTGCA GAAGGTGGAG 
TGCTTGAAAA AAAAGAAATC CTTGCCACTG GCCTGCCAAA ACAACAACAA TAACAGCAAT 
TTGCCGAATA ACAACAATGT GGAGTCGCTT AAGAAACCGG TGGTGCAGGG AACGAGCTAC 
AATCAGACTC ATCCGCCACC GCTGATGGTT TTCAATACGG GAACAGTTGC AGTTCCCGCG 
CAGAGTCCGC AGACTGCTGC TCCACAGAAA CATTCCACCG GCAACAGCGT AGATGACAGC 
GATCTCAACG AGATACCCGT CAATGTTATC TTCAGAAAGC CGCAAGAGGC AGGCGGAGCG 
GCGAAAACAG GTGGACCGGG AGGATTAAGT GCACCTGTTT CGGGAACGCC GCAAACTCGT 
CCAGCTGAAG TGAAAATGGT GACTCCTCTC ACGCCGCCCA CTCCACCAGA GATGAGCGCA 
CCGCCCCTGC TAGCGCAAAT GCAACCCCCG CAGATACCCA CGTCTTGTGT TCCAGCTTTA 
GCTCCCAGCT TCAAAGTGTC GTCACCAGCA GTTCTCAGCC CGAAGGTGAT CTCACCAGCT 
CCTGCAAGCC CAAAGCTCTT GTGTCCGACA GCACCCGCTT CAACGAACTC ACTGCAAATT 
GCGCCAAAAG CTGTTCCAGC CACTACAACC TCATCTGCAC CAGCATTAGC CACGAAATCA 
GAACAAATGT CTTCCAAAGT GGCCAATTTA AACGCCTTCA ACCGTCAAGC ACCCATAGCT 
TCAAAGGGCG TTAGAAATGG CATCACTAAC AACAATAACA ACAGAAACAG CAATGTCGTT 
GCGAAGAAAT CGTCACCAAC TTCGATGCCG CCTCCAAAGG ATCCGATTGC ACCGATTGCA 
GCAAACGAGT TGACGGATTC CGAACATCGT CAGCGACGCC GCAAGCCCGC CTCCGCCTGC 
AGACGCAGCT TGGACACTCT GAGTGAGAAC GAGTCCTTTT CAGTGGATTC GCCCTACTGC 
CTACAACAGC ATTGGCTGCA CTCCGGCTTT AATAATAAGT CCCACGACGC GCCACTATCG 
CAAAGTAACC GGCGTGAGGA TCGAATTGCT GTTCGTAAAG GAGCCCTGAG GCGACAAGCG 
CTGCAGCTAC TGTCTACGCG CTCCCTGCAG GAGCTTCCCA TGCGAGCGGC CAAACAGCGG 
CTGCAGTGCG TCCAGAACAT GCTGATCAAG TACCAAGATC ACGCTGGCCA GCAGCAGGGA 
ATTGGAATTG GCAACCACTG TCTGGTGGCC AGCTGCAAGC AGCCCACGCT TAGTATGGCA 
GCGCACTGCG AGCGTCACAT CGTGAACAAC AGCACCCAGC AGCTTTTCCA GCCTTGCGTC 
GCCTGGCGGA TGGATGGAAC CGCTTGCCAG GCTCCTGTCT TCGATGTGCT GCACACATTG 
GCGCTCTGCA AGGTGCACAG CCACCTGCGT TCTGGTATGG ATGGAGCCCG TCCAGCATCG 
AAACAGCCGC CCGTTAGTTT ACCAGTCGCT GGAGTGGCGA CTCTTTATGT GCCTGTGAAG 
CAGCAGCGAA AGCGCAAGGC TAATACGAAT GCCGTGGCTC GTCCTCAGAA ACGTGGTAGG 
AAGCCGGCGA ATGAACCGAT TGCTAACCAG ATCAGCAGCC AGATAAACAA ACTGATACCT 
GGGGCAGGAA TGCAGCGTAA AAGCAGCACC ACATCGCTTG AGTCCATTGC CAGTAATTCG 
CAATCGTCGG CCACCTCACA CTCACAGCCG CCTTACAAGC CCGGAAATGT GTTGGCCGCT 
GTACCTGCAG CACAGAACTT GTCCCAACGG TCCATTCCTC CAGCGCTGGC TCCGCTCAGC 
AGTGATTTTC AACCCAACCA TCAGCAGCAG GAACCGCTTT TGGTTCCCAA GTTGGAGGTG 
GATTCATTGT TTAAATTTGA TGCCGATCAA CAGCAAAATC AGCAGCAGCA ATCGACTTTG 
GACCCCAGCT TACTTTCTTT GGACATTGCG AATATCAAGG CCGAGGAGAT TAGCCAAATT 
GTAGCACAAT TGGCGGCGGC CGGTGGTGAT TTACAAAATC CCAACAATAA TAATAACAAC 
AACATAGGAA TCCATAATAA CAATAGCGTG CACTTCAACA ACAACAACAA CAATAGTATG 
AACTACAGCA ACAATAACAA TAACAGTTTC CCCTCATTCA ACACGGCCTT TGGCAACGCC 
ATGGGCCAGC CAAGCAACAC AATTACGCAC AATGGCCACG CTGTTCCGGC AGTGCTGGCC 
AATACGGCCG ATCTTCTTGG CCAGGACATG TTTGGTATTT GCGAGAACAG CTCGGCATAC 
GCCAGTTCCG AGGATACCGG ATTGGGCGGT CTCAGCGAGT CGGAGCTGAT AGGCACTAAC 
GATGCTGATG ATATAGCATT GAATGGCGCC CACTTGCTGG AGGAGCACGA TCTGGTAAAT 
GTGTTCGACA CGCTGTCAGA CGATGCCTTC AACGAGCTGT TCCAATCCGT GCAACAAGCC 
GAGTGCGAGG CTATGGACCG GGCTTTGGAC CGGGCCTTAC AGCAGACAAT GGGCGGCTCG 
GCGGACAGCG CCTTTCTTAA CGATTTCCTG GACGTCGGCG ACGATCTGCT GGCCGATGCT 
GTGATGCACT CACCAAACAC GTCCGGCATC GATGCTCCTC CCCTCTTTGG GGACAGCAGC 
AGCGGCGGTG GCAATAGCAG CAGCAACGGC GCCTCCGACA TCCGGGGCTT GGTGCAGACC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

l(3)L1231 ( NM_001260175.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247104.1 Drosophila melanogaster lethal (3) L1231 (l(3)L1231), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04889
Clone ID Related Accession (Same CDS sequence)	NM_001300399.1 , NM_001260175.2 , NM_142122.4
Accession Version	NM_001260175.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (3) L1231, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260175.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATGTC AGCAAGCCTC CAGATTCCAT TCAGCCACCG CTACAGCCAC ATTAGCCAAG 
AGCACAGCCA CCAGAAGGAT TGCCACAGCA GCAGCAGCCG CAACAGCAAC AGCGATAGCC 
GCAGCAACAG CCGCAGCAGC AGCAGCAACG CTGACGCCCG TGGAGAGCAT TGCCGGCAAG 
ACGACGTCCG AGGACTCGGA TCCCTATGCC TTCACCGAGA CTGTGGCCGT CACACCACCC 
ATTCTATTCA ATGCACAGAA ATCGAGAGCC CGCCTAACCG ACAGCAATAG AGGCAGCAAC 
AAGAGGCAGA CGGCAGCAAC AGCTGCGGCC AACAGAAAGG CGAACCTGGT GGCCCAACTG 
AGTGTCACAG AGGCAGCAAA GGCGCAGGCG TCTTTGGCAA GCAACAACAC AACGAATTTC 
CATCATGTCA CGCAATCTCA GAGACAGTCG ACGGCGCTGC AGTTGCAATT GCCACTGCAA 
TCCCAGTCAC AGTCGCAGGC CTCGCCGAAG CGGGCCACCA ACGTGTGCAT AGTCCGCCCG 
CAGCAACAGC AGCTGGAGAA GATAGCCACC TCGGAGTCCT GCCAGTCGCC GGCAGCACCA 
CCACCGCTTT ACGCCCACAC TCCATCGCTG TGGCAGACGC CGCTGCTCAT AGACAATGGG 
CAAAAGCAAC AGCTCCTCCA GCAGCAGCAT CAGCAACCGC AACAGCAACA GTCCGTTGCT 
ATTGCGTTGG TCAGTCCGCC CACATCGCCC GCCTCATTAC CTTCGCCCAC TCTGCCGCCT 
GCCACCGCTG CAGTGACCGC CATGGTGGCA CCGATTTCCG TATCGCCCAA GGGTGGATTA 
CCTTTGCCGC CATCGAAGTT CCATCACACC ACACTGGCGC AACATCTGCA GAAGGTGGAG 
TGCTTGAAAA AAAAGAAATC CTTGCCACTG GCCTGCCAAA ACAACAACAA TAACAGCAAT 
TTGCCGAATA ACAACAATGT GGAGTCGCTT AAGAAACCGG TGGTGCAGGG AACGAGCTAC 
AATCAGACTC ATCCGCCACC GCTGATGGTT TTCAATACGG GAACAGTTGC AGTTCCCGCG 
CAGAGTCCGC AGACTGCTGC TCCACAGAAA CATTCCACCG GCAACAGCGT AGATGACAGC 
GATCTCAACG AGATACCCGT CAATGTTATC TTCAGAAAGC CGCAAGAGGC AGGCGGAGCG 
GCGAAAACAG GTGGACCGGG AGGATTAAGT GCACCTGTTT CGGGAACGCC GCAAACTCGT 
CCAGCTGAAG TGAAAATGGT GACTCCTCTC ACGCCGCCCA CTCCACCAGA GATGAGCGCA 
CCGCCCCTGC TAGCGCAAAT GCAACCCCCG CAGATACCCA CGTCTTGTGT TCCAGCTTTA 
GCTCCCAGCT TCAAAGTGTC GTCACCAGCA GTTCTCAGCC CGAAGGTGAT CTCACCAGCT 
CCTGCAAGCC CAAAGCTCTT GTGTCCGACA GCACCCGCTT CAACGAACTC ACTGCAAATT 
GCGCCAAAAG CTGTTCCAGC CACTACAACC TCATCTGCAC CAGCATTAGC CACGAAATCA 
GAACAAATGT CTTCCAAAGT GGCCAATTTA AACGCCTTCA ACCGTCAAGC ACCCATAGCT 
TCAAAGGGCG TTAGAAATGG CATCACTAAC AACAATAACA ACAGAAACAG CAATGTCGTT 
GCGAAGAAAT CGTCACCAAC TTCGATGCCG CCTCCAAAGG ATCCGATTGC ACCGATTGCA 
GCAAACGAGT TGACGGATTC CGAACATCGT CAGCGACGCC GCAAGCCCGC CTCCGCCTGC 
AGACGCAGCT TGGACACTCT GAGTGAGAAC GAGTCCTTTT CAGTGGATTC GCCCTACTGC 
CTACAACAGC ATTGGCTGCA CTCCGGCTTT AATAATAAGT CCCACGACGC GCCACTATCG 
CAAAGTAACC GGCGTGAGGA TCGAATTGCT GTTCGTAAAG GAGCCCTGAG GCGACAAGCG 
CTGCAGCTAC TGTCTACGCG CTCCCTGCAG GAGCTTCCCA TGCGAGCGGC CAAACAGCGG 
CTGCAGTGCG TCCAGAACAT GCTGATCAAG TACCAAGATC ACGCTGGCCA GCAGCAGGGA 
ATTGGAATTG GCAACCACTG TCTGGTGGCC AGCTGCAAGC AGCCCACGCT TAGTATGGCA 
GCGCACTGCG AGCGTCACAT CGTGAACAAC AGCACCCAGC AGCTTTTCCA GCCTTGCGTC 
GCCTGGCGGA TGGATGGAAC CGCTTGCCAG GCTCCTGTCT TCGATGTGCT GCACACATTG 
GCGCTCTGCA AGGTGCACAG CCACCTGCGT TCTGGTATGG ATGGAGCCCG TCCAGCATCG 
AAACAGCCGC CCGTTAGTTT ACCAGTCGCT GGAGTGGCGA CTCTTTATGT GCCTGTGAAG 
CAGCAGCGAA AGCGCAAGGC TAATACGAAT GCCGTGGCTC GTCCTCAGAA ACGTGGTAGG 
AAGCCGGCGA ATGAACCGAT TGCTAACCAG ATCAGCAGCC AGATAAACAA ACTGATACCT 
GGGGCAGGAA TGCAGCGTAA AAGCAGCACC ACATCGCTTG AGTCCATTGC CAGTAATTCG 
CAATCGTCGG CCACCTCACA CTCACAGCCG CCTTACAAGC CCGGAAATGT GTTGGCCGCT 
GTACCTGCAG CACAGAACTT GTCCCAACGG TCCATTCCTC CAGCGCTGGC TCCGCTCAGC 
AGTGATTTTC AACCCAACCA TCAGCAGCAG GAACCGCTTT TGGTTCCCAA GTTGGAGGTG 
GATTCATTGT TTAAATTTGA TGCCGATCAA CAGCAAAATC AGCAGCAGCA ATCGACTTTG 
GACCCCAGCT TACTTTCTTT GGACATTGCG AATATCAAGG CCGAGGAGAT TAGCCAAATT 
GTAGCACAAT TGGCGGCGGC CGGTGGTGAT TTACAAAATC CCAACAATAA TAATAACAAC 
AACATAGGAA TCCATAATAA CAATAGCGTG CACTTCAACA ACAACAACAA CAATAGTATG 
AACTACAGCA ACAATAACAA TAACAGTTTC CCCTCATTCA ACACGGCCTT TGGCAACGCC 
ATGGGCCAGC CAAGCAACAC AATTACGCAC AATGGCCACG CTGTTCCGGC AGTGCTGGCC 
AATACGGCCG ATCTTCTTGG CCAGGACATG TTTGGTATTT GCGAGAACAG CTCGGCATAC 
GCCAGTTCCG AGGATACCGG ATTGGGCGGT CTCAGCGAGT CGGAGCTGAT AGGCACTAAC 
GATGCTGATG ATATAGCATT GAATGGCGCC CACTTGCTGG AGGAGCACGA TCTGGTAAAT 
GTGTTCGACA CGCTGTCAGA CGATGCCTTC AACGAGCTGT TCCAATCCGT GCAACAAGCC 
GAGTGCGAGG CTATGGACCG GGCTTTGGAC CGGGCCTTAC AGCAGACAAT GGGCGGCTCG 
GCGGACAGCG CCTTTCTTAA CGATTTCCTG GACGTCGGCG ACGATCTGCT GGCCGATGCT 
GTGATGCACT CACCAAACAC GTCCGGCATC GATGCTCCTC CCCTCTTTGG GGACAGCAGC 
AGCGGCGGTG GCAATAGCAG CAGCAACGGC GCCTCCGACA TCCGGGGCTT GGTGCAGACC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247104.1
CDS	142..3744
Translation	MSCQQASRFHSATATATLAKSTATRRIATAAAAATATAIAAATAAAAAATLTPVESIAGKTTSEDSDPYAFTETVAVTPPILFNAQKSRARLTDSNRGSNKRQTAATAAANRKANLVAQLSVTEAAKAQASLASNNTTNFHHVTQSQRQSTALQLQLPLQSQSQSQASPKRATNVCIVRPQQQQLEKIATSESCQSPAAPPPLYAHTPSLWQTPLLIDNGQKQQLLQQQHQQPQQQQSVAIALVSPPTSPASLPSPTLPPATAAVTAMVAPISVSPKGGLPLPPSKFHHTTLAQHLQKVECLKKKKSLPLACQNNNNNSNLPNNNNVESLKKPVVQGTSYNQTHPPPLMVFNTGTVAVPAQSPQTAAPQKHSTGNSVDDSDLNEIPVNVIFRKPQEAGGAAKTGGPGGLSAPVSGTPQTRPAEVKMVTPLTPPTPPEMSAPPLLAQMQPPQIPTSCVPALAPSFKVSSPAVLSPKVISPAPASPKLLCPTAPASTNSLQIAPKAVPATTTSSAPALATKSEQMSSKVANLNAFNRQAPIASKGVRNGITNNNNNRNSNVVAKKSSPTSMPPPKDPIAPIAANELTDSEHRQRRRKPASACRRSLDTLSENESFSVDSPYCLQQHWLHSGFNNKSHDAPLSQSNRREDRIAVRKGALRRQALQLLSTRSLQELPMRAAKQRLQCVQNMLIKYQDHAGQQQGIGIGNHCLVASCKQPTLSMAAHCERHIVNNSTQQLFQPCVAWRMDGTACQAPVFDVLHTLALCKVHSHLRSGMDGARPASKQPPVSLPVAGVATLYVPVKQQRKRKANTNAVARPQKRGRKPANEPIANQISSQINKLIPGAGMQRKSSTTSLESIASNSQSSATSHSQPPYKPGNVLAAVPAAQNLSQRSIPPALAPLSSDFQPNHQQQEPLLVPKLEVDSLFKFDADQQQNQQQQSTLDPSLLSLDIANIKAEEISQIVAQLAAAGGDLQNPNNNNNNNIGIHNNNSVHFNNNNNNSMNYSNNNNNSFPSFNTAFGNAMGQPSNTITHNGHAVPAVLANTADLLGQDMFGICENSSAYASSEDTGLGGLSESELIGTNDADDIALNGAHLLEEHDLVNVFDTLSDDAFNELFQSVQQAECEAMDRALDRALQQTMGGSADSAFLNDFLDVGDDLLADAVMHSPNTSGIDAPPLFGDSSSGGGNSSSNGASDIRGLVQT

Target ORF information:

RefSeq Version	NM_001260175.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (3) L1231 (l(3)L1231), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260175.2

ORF Insert Sequence:

ATGTCATGTC AGCAAGCCTC CAGATTCCAT TCAGCCACCG CTACAGCCAC ATTAGCCAAG 
AGCACAGCCA CCAGAAGGAT TGCCACAGCA GCAGCAGCCG CAACAGCAAC AGCGATAGCC 
GCAGCAACAG CCGCAGCAGC AGCAGCAACG CTGACGCCCG TGGAGAGCAT TGCCGGCAAG 
ACGACGTCCG AGGACTCGGA TCCCTATGCC TTCACCGAGA CTGTGGCCGT CACACCACCC 
ATTCTATTCA ATGCACAGAA ATCGAGAGCC CGCCTAACCG ACAGCAATAG AGGCAGCAAC 
AAGAGGCAGA CGGCAGCAAC AGCTGCGGCC AACAGAAAGG CGAACCTGGT GGCCCAACTG 
AGTGTCACAG AGGCAGCAAA GGCGCAGGCG TCTTTGGCAA GCAACAACAC AACGAATTTC 
CATCATGTCA CGCAATCTCA GAGACAGTCG ACGGCGCTGC AGTTGCAATT GCCACTGCAA 
TCCCAGTCAC AGTCGCAGGC CTCGCCGAAG CGGGCCACCA ACGTGTGCAT AGTCCGCCCG 
CAGCAACAGC AGCTGGAGAA GATAGCCACC TCGGAGTCCT GCCAGTCGCC GGCAGCACCA 
CCACCGCTTT ACGCCCACAC TCCATCGCTG TGGCAGACGC CGCTGCTCAT AGACAATGGG 
CAAAAGCAAC AGCTCCTCCA GCAGCAGCAT CAGCAACCGC AACAGCAACA GTCCGTTGCT 
ATTGCGTTGG TCAGTCCGCC CACATCGCCC GCCTCATTAC CTTCGCCCAC TCTGCCGCCT 
GCCACCGCTG CAGTGACCGC CATGGTGGCA CCGATTTCCG TATCGCCCAA GGGTGGATTA 
CCTTTGCCGC CATCGAAGTT CCATCACACC ACACTGGCGC AACATCTGCA GAAGGTGGAG 
TGCTTGAAAA AAAAGAAATC CTTGCCACTG GCCTGCCAAA ACAACAACAA TAACAGCAAT 
TTGCCGAATA ACAACAATGT GGAGTCGCTT AAGAAACCGG TGGTGCAGGG AACGAGCTAC 
AATCAGACTC ATCCGCCACC GCTGATGGTT TTCAATACGG GAACAGTTGC AGTTCCCGCG 
CAGAGTCCGC AGACTGCTGC TCCACAGAAA CATTCCACCG GCAACAGCGT AGATGACAGC 
GATCTCAACG AGATACCCGT CAATGTTATC TTCAGAAAGC CGCAAGAGGC AGGCGGAGCG 
GCGAAAACAG GTGGACCGGG AGGATTAAGT GCACCTGTTT CGGGAACGCC GCAAACTCGT 
CCAGCTGAAG TGAAAATGGT GACTCCTCTC ACGCCGCCCA CTCCACCAGA GATGAGCGCA 
CCGCCCCTGC TAGCGCAAAT GCAACCCCCG CAGATACCCA CGTCTTGTGT TCCAGCTTTA 
GCTCCCAGCT TCAAAGTGTC GTCACCAGCA GTTCTCAGCC CGAAGGTGAT CTCACCAGCT 
CCTGCAAGCC CAAAGCTCTT GTGTCCGACA GCACCCGCTT CAACGAACTC ACTGCAAATT 
GCGCCAAAAG CTGTTCCAGC CACTACAACC TCATCTGCAC CAGCATTAGC CACGAAATCA 
GAACAAATGT CTTCCAAAGT GGCCAATTTA AACGCCTTCA ACCGTCAAGC ACCCATAGCT 
TCAAAGGGCG TTAGAAATGG CATCACTAAC AACAATAACA ACAGAAACAG CAATGTCGTT 
GCGAAGAAAT CGTCACCAAC TTCGATGCCG CCTCCAAAGG ATCCGATTGC ACCGATTGCA 
GCAAACGAGT TGACGGATTC CGAACATCGT CAGCGACGCC GCAAGCCCGC CTCCGCCTGC 
AGACGCAGCT TGGACACTCT GAGTGAGAAC GAGTCCTTTT CAGTGGATTC GCCCTACTGC 
CTACAACAGC ATTGGCTGCA CTCCGGCTTT AATAATAAGT CCCACGACGC GCCACTATCG 
CAAAGTAACC GGCGTGAGGA TCGAATTGCT GTTCGTAAAG GAGCCCTGAG GCGACAAGCG 
CTGCAGCTAC TGTCTACGCG CTCCCTGCAG GAGCTTCCCA TGCGAGCGGC CAAACAGCGG 
CTGCAGTGCG TCCAGAACAT GCTGATCAAG TACCAAGATC ACGCTGGCCA GCAGCAGGGA 
ATTGGAATTG GCAACCACTG TCTGGTGGCC AGCTGCAAGC AGCCCACGCT TAGTATGGCA 
GCGCACTGCG AGCGTCACAT CGTGAACAAC AGCACCCAGC AGCTTTTCCA GCCTTGCGTC 
GCCTGGCGGA TGGATGGAAC CGCTTGCCAG GCTCCTGTCT TCGATGTGCT GCACACATTG 
GCGCTCTGCA AGGTGCACAG CCACCTGCGT TCTGGTATGG ATGGAGCCCG TCCAGCATCG 
AAACAGCCGC CCGTTAGTTT ACCAGTCGCT GGAGTGGCGA CTCTTTATGT GCCTGTGAAG 
CAGCAGCGAA AGCGCAAGGC TAATACGAAT GCCGTGGCTC GTCCTCAGAA ACGTGGTAGG 
AAGCCGGCGA ATGAACCGAT TGCTAACCAG ATCAGCAGCC AGATAAACAA ACTGATACCT 
GGGGCAGGAA TGCAGCGTAA AAGCAGCACC ACATCGCTTG AGTCCATTGC CAGTAATTCG 
CAATCGTCGG CCACCTCACA CTCACAGCCG CCTTACAAGC CCGGAAATGT GTTGGCCGCT 
GTACCTGCAG CACAGAACTT GTCCCAACGG TCCATTCCTC CAGCGCTGGC TCCGCTCAGC 
AGTGATTTTC AACCCAACCA TCAGCAGCAG GAACCGCTTT TGGTTCCCAA GTTGGAGGTG 
GATTCATTGT TTAAATTTGA TGCCGATCAA CAGCAAAATC AGCAGCAGCA ATCGACTTTG 
GACCCCAGCT TACTTTCTTT GGACATTGCG AATATCAAGG CCGAGGAGAT TAGCCAAATT 
GTAGCACAAT TGGCGGCGGC CGGTGGTGAT TTACAAAATC CCAACAATAA TAATAACAAC 
AACATAGGAA TCCATAATAA CAATAGCGTG CACTTCAACA ACAACAACAA CAATAGTATG 
AACTACAGCA ACAATAACAA TAACAGTTTC CCCTCATTCA ACACGGCCTT TGGCAACGCC 
ATGGGCCAGC CAAGCAACAC AATTACGCAC AATGGCCACG CTGTTCCGGC AGTGCTGGCC 
AATACGGCCG ATCTTCTTGG CCAGGACATG TTTGGTATTT GCGAGAACAG CTCGGCATAC 
GCCAGTTCCG AGGATACCGG ATTGGGCGGT CTCAGCGAGT CGGAGCTGAT AGGCACTAAC 
GATGCTGATG ATATAGCATT GAATGGCGCC CACTTGCTGG AGGAGCACGA TCTGGTAAAT 
GTGTTCGACA CGCTGTCAGA CGATGCCTTC AACGAGCTGT TCCAATCCGT GCAACAAGCC 
GAGTGCGAGG CTATGGACCG GGCTTTGGAC CGGGCCTTAC AGCAGACAAT GGGCGGCTCG 
GCGGACAGCG CCTTTCTTAA CGATTTCCTG GACGTCGGCG ACGATCTGCT GGCCGATGCT 
GTGATGCACT CACCAAACAC GTCCGGCATC GATGCTCCTC CCCTCTTTGG GGACAGCAGC 
AGCGGCGGTG GCAATAGCAG CAGCAACGGC GCCTCCGACA TCCGGGGCTT GGTGCAGACC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

l(3)L1231 ( NM_142122.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650379.1 Drosophila melanogaster lethal (3) L1231 (l(3)L1231), transcript variant A, mRNA.

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CloneID	OFa04889
Clone ID Related Accession (Same CDS sequence)	NM_001300399.1 , NM_001260175.2 , NM_142122.4
Accession Version	NM_142122.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (3) L1231, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142122.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATGTC AGCAAGCCTC CAGATTCCAT TCAGCCACCG CTACAGCCAC ATTAGCCAAG 
AGCACAGCCA CCAGAAGGAT TGCCACAGCA GCAGCAGCCG CAACAGCAAC AGCGATAGCC 
GCAGCAACAG CCGCAGCAGC AGCAGCAACG CTGACGCCCG TGGAGAGCAT TGCCGGCAAG 
ACGACGTCCG AGGACTCGGA TCCCTATGCC TTCACCGAGA CTGTGGCCGT CACACCACCC 
ATTCTATTCA ATGCACAGAA ATCGAGAGCC CGCCTAACCG ACAGCAATAG AGGCAGCAAC 
AAGAGGCAGA CGGCAGCAAC AGCTGCGGCC AACAGAAAGG CGAACCTGGT GGCCCAACTG 
AGTGTCACAG AGGCAGCAAA GGCGCAGGCG TCTTTGGCAA GCAACAACAC AACGAATTTC 
CATCATGTCA CGCAATCTCA GAGACAGTCG ACGGCGCTGC AGTTGCAATT GCCACTGCAA 
TCCCAGTCAC AGTCGCAGGC CTCGCCGAAG CGGGCCACCA ACGTGTGCAT AGTCCGCCCG 
CAGCAACAGC AGCTGGAGAA GATAGCCACC TCGGAGTCCT GCCAGTCGCC GGCAGCACCA 
CCACCGCTTT ACGCCCACAC TCCATCGCTG TGGCAGACGC CGCTGCTCAT AGACAATGGG 
CAAAAGCAAC AGCTCCTCCA GCAGCAGCAT CAGCAACCGC AACAGCAACA GTCCGTTGCT 
ATTGCGTTGG TCAGTCCGCC CACATCGCCC GCCTCATTAC CTTCGCCCAC TCTGCCGCCT 
GCCACCGCTG CAGTGACCGC CATGGTGGCA CCGATTTCCG TATCGCCCAA GGGTGGATTA 
CCTTTGCCGC CATCGAAGTT CCATCACACC ACACTGGCGC AACATCTGCA GAAGGTGGAG 
TGCTTGAAAA AAAAGAAATC CTTGCCACTG GCCTGCCAAA ACAACAACAA TAACAGCAAT 
TTGCCGAATA ACAACAATGT GGAGTCGCTT AAGAAACCGG TGGTGCAGGG AACGAGCTAC 
AATCAGACTC ATCCGCCACC GCTGATGGTT TTCAATACGG GAACAGTTGC AGTTCCCGCG 
CAGAGTCCGC AGACTGCTGC TCCACAGAAA CATTCCACCG GCAACAGCGT AGATGACAGC 
GATCTCAACG AGATACCCGT CAATGTTATC TTCAGAAAGC CGCAAGAGGC AGGCGGAGCG 
GCGAAAACAG GTGGACCGGG AGGATTAAGT GCACCTGTTT CGGGAACGCC GCAAACTCGT 
CCAGCTGAAG TGAAAATGGT GACTCCTCTC ACGCCGCCCA CTCCACCAGA GATGAGCGCA 
CCGCCCCTGC TAGCGCAAAT GCAACCCCCG CAGATACCCA CGTCTTGTGT TCCAGCTTTA 
GCTCCCAGCT TCAAAGTGTC GTCACCAGCA GTTCTCAGCC CGAAGGTGAT CTCACCAGCT 
CCTGCAAGCC CAAAGCTCTT GTGTCCGACA GCACCCGCTT CAACGAACTC ACTGCAAATT 
GCGCCAAAAG CTGTTCCAGC CACTACAACC TCATCTGCAC CAGCATTAGC CACGAAATCA 
GAACAAATGT CTTCCAAAGT GGCCAATTTA AACGCCTTCA ACCGTCAAGC ACCCATAGCT 
TCAAAGGGCG TTAGAAATGG CATCACTAAC AACAATAACA ACAGAAACAG CAATGTCGTT 
GCGAAGAAAT CGTCACCAAC TTCGATGCCG CCTCCAAAGG ATCCGATTGC ACCGATTGCA 
GCAAACGAGT TGACGGATTC CGAACATCGT CAGCGACGCC GCAAGCCCGC CTCCGCCTGC 
AGACGCAGCT TGGACACTCT GAGTGAGAAC GAGTCCTTTT CAGTGGATTC GCCCTACTGC 
CTACAACAGC ATTGGCTGCA CTCCGGCTTT AATAATAAGT CCCACGACGC GCCACTATCG 
CAAAGTAACC GGCGTGAGGA TCGAATTGCT GTTCGTAAAG GAGCCCTGAG GCGACAAGCG 
CTGCAGCTAC TGTCTACGCG CTCCCTGCAG GAGCTTCCCA TGCGAGCGGC CAAACAGCGG 
CTGCAGTGCG TCCAGAACAT GCTGATCAAG TACCAAGATC ACGCTGGCCA GCAGCAGGGA 
ATTGGAATTG GCAACCACTG TCTGGTGGCC AGCTGCAAGC AGCCCACGCT TAGTATGGCA 
GCGCACTGCG AGCGTCACAT CGTGAACAAC AGCACCCAGC AGCTTTTCCA GCCTTGCGTC 
GCCTGGCGGA TGGATGGAAC CGCTTGCCAG GCTCCTGTCT TCGATGTGCT GCACACATTG 
GCGCTCTGCA AGGTGCACAG CCACCTGCGT TCTGGTATGG ATGGAGCCCG TCCAGCATCG 
AAACAGCCGC CCGTTAGTTT ACCAGTCGCT GGAGTGGCGA CTCTTTATGT GCCTGTGAAG 
CAGCAGCGAA AGCGCAAGGC TAATACGAAT GCCGTGGCTC GTCCTCAGAA ACGTGGTAGG 
AAGCCGGCGA ATGAACCGAT TGCTAACCAG ATCAGCAGCC AGATAAACAA ACTGATACCT 
GGGGCAGGAA TGCAGCGTAA AAGCAGCACC ACATCGCTTG AGTCCATTGC CAGTAATTCG 
CAATCGTCGG CCACCTCACA CTCACAGCCG CCTTACAAGC CCGGAAATGT GTTGGCCGCT 
GTACCTGCAG CACAGAACTT GTCCCAACGG TCCATTCCTC CAGCGCTGGC TCCGCTCAGC 
AGTGATTTTC AACCCAACCA TCAGCAGCAG GAACCGCTTT TGGTTCCCAA GTTGGAGGTG 
GATTCATTGT TTAAATTTGA TGCCGATCAA CAGCAAAATC AGCAGCAGCA ATCGACTTTG 
GACCCCAGCT TACTTTCTTT GGACATTGCG AATATCAAGG CCGAGGAGAT TAGCCAAATT 
GTAGCACAAT TGGCGGCGGC CGGTGGTGAT TTACAAAATC CCAACAATAA TAATAACAAC 
AACATAGGAA TCCATAATAA CAATAGCGTG CACTTCAACA ACAACAACAA CAATAGTATG 
AACTACAGCA ACAATAACAA TAACAGTTTC CCCTCATTCA ACACGGCCTT TGGCAACGCC 
ATGGGCCAGC CAAGCAACAC AATTACGCAC AATGGCCACG CTGTTCCGGC AGTGCTGGCC 
AATACGGCCG ATCTTCTTGG CCAGGACATG TTTGGTATTT GCGAGAACAG CTCGGCATAC 
GCCAGTTCCG AGGATACCGG ATTGGGCGGT CTCAGCGAGT CGGAGCTGAT AGGCACTAAC 
GATGCTGATG ATATAGCATT GAATGGCGCC CACTTGCTGG AGGAGCACGA TCTGGTAAAT 
GTGTTCGACA CGCTGTCAGA CGATGCCTTC AACGAGCTGT TCCAATCCGT GCAACAAGCC 
GAGTGCGAGG CTATGGACCG GGCTTTGGAC CGGGCCTTAC AGCAGACAAT GGGCGGCTCG 
GCGGACAGCG CCTTTCTTAA CGATTTCCTG GACGTCGGCG ACGATCTGCT GGCCGATGCT 
GTGATGCACT CACCAAACAC GTCCGGCATC GATGCTCCTC CCCTCTTTGG GGACAGCAGC 
AGCGGCGGTG GCAATAGCAG CAGCAACGGC GCCTCCGACA TCCGGGGCTT GGTGCAGACC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650379.1
CDS	359..3961
Translation	MSCQQASRFHSATATATLAKSTATRRIATAAAAATATAIAAATAAAAAATLTPVESIAGKTTSEDSDPYAFTETVAVTPPILFNAQKSRARLTDSNRGSNKRQTAATAAANRKANLVAQLSVTEAAKAQASLASNNTTNFHHVTQSQRQSTALQLQLPLQSQSQSQASPKRATNVCIVRPQQQQLEKIATSESCQSPAAPPPLYAHTPSLWQTPLLIDNGQKQQLLQQQHQQPQQQQSVAIALVSPPTSPASLPSPTLPPATAAVTAMVAPISVSPKGGLPLPPSKFHHTTLAQHLQKVECLKKKKSLPLACQNNNNNSNLPNNNNVESLKKPVVQGTSYNQTHPPPLMVFNTGTVAVPAQSPQTAAPQKHSTGNSVDDSDLNEIPVNVIFRKPQEAGGAAKTGGPGGLSAPVSGTPQTRPAEVKMVTPLTPPTPPEMSAPPLLAQMQPPQIPTSCVPALAPSFKVSSPAVLSPKVISPAPASPKLLCPTAPASTNSLQIAPKAVPATTTSSAPALATKSEQMSSKVANLNAFNRQAPIASKGVRNGITNNNNNRNSNVVAKKSSPTSMPPPKDPIAPIAANELTDSEHRQRRRKPASACRRSLDTLSENESFSVDSPYCLQQHWLHSGFNNKSHDAPLSQSNRREDRIAVRKGALRRQALQLLSTRSLQELPMRAAKQRLQCVQNMLIKYQDHAGQQQGIGIGNHCLVASCKQPTLSMAAHCERHIVNNSTQQLFQPCVAWRMDGTACQAPVFDVLHTLALCKVHSHLRSGMDGARPASKQPPVSLPVAGVATLYVPVKQQRKRKANTNAVARPQKRGRKPANEPIANQISSQINKLIPGAGMQRKSSTTSLESIASNSQSSATSHSQPPYKPGNVLAAVPAAQNLSQRSIPPALAPLSSDFQPNHQQQEPLLVPKLEVDSLFKFDADQQQNQQQQSTLDPSLLSLDIANIKAEEISQIVAQLAAAGGDLQNPNNNNNNNIGIHNNNSVHFNNNNNNSMNYSNNNNNSFPSFNTAFGNAMGQPSNTITHNGHAVPAVLANTADLLGQDMFGICENSSAYASSEDTGLGGLSESELIGTNDADDIALNGAHLLEEHDLVNVFDTLSDDAFNELFQSVQQAECEAMDRALDRALQQTMGGSADSAFLNDFLDVGDDLLADAVMHSPNTSGIDAPPLFGDSSSGGGNSSSNGASDIRGLVQT

Target ORF information:

RefSeq Version	NM_142122.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (3) L1231 (l(3)L1231), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142122.4

ORF Insert Sequence:

ATGTCATGTC AGCAAGCCTC CAGATTCCAT TCAGCCACCG CTACAGCCAC ATTAGCCAAG 
AGCACAGCCA CCAGAAGGAT TGCCACAGCA GCAGCAGCCG CAACAGCAAC AGCGATAGCC 
GCAGCAACAG CCGCAGCAGC AGCAGCAACG CTGACGCCCG TGGAGAGCAT TGCCGGCAAG 
ACGACGTCCG AGGACTCGGA TCCCTATGCC TTCACCGAGA CTGTGGCCGT CACACCACCC 
ATTCTATTCA ATGCACAGAA ATCGAGAGCC CGCCTAACCG ACAGCAATAG AGGCAGCAAC 
AAGAGGCAGA CGGCAGCAAC AGCTGCGGCC AACAGAAAGG CGAACCTGGT GGCCCAACTG 
AGTGTCACAG AGGCAGCAAA GGCGCAGGCG TCTTTGGCAA GCAACAACAC AACGAATTTC 
CATCATGTCA CGCAATCTCA GAGACAGTCG ACGGCGCTGC AGTTGCAATT GCCACTGCAA 
TCCCAGTCAC AGTCGCAGGC CTCGCCGAAG CGGGCCACCA ACGTGTGCAT AGTCCGCCCG 
CAGCAACAGC AGCTGGAGAA GATAGCCACC TCGGAGTCCT GCCAGTCGCC GGCAGCACCA 
CCACCGCTTT ACGCCCACAC TCCATCGCTG TGGCAGACGC CGCTGCTCAT AGACAATGGG 
CAAAAGCAAC AGCTCCTCCA GCAGCAGCAT CAGCAACCGC AACAGCAACA GTCCGTTGCT 
ATTGCGTTGG TCAGTCCGCC CACATCGCCC GCCTCATTAC CTTCGCCCAC TCTGCCGCCT 
GCCACCGCTG CAGTGACCGC CATGGTGGCA CCGATTTCCG TATCGCCCAA GGGTGGATTA 
CCTTTGCCGC CATCGAAGTT CCATCACACC ACACTGGCGC AACATCTGCA GAAGGTGGAG 
TGCTTGAAAA AAAAGAAATC CTTGCCACTG GCCTGCCAAA ACAACAACAA TAACAGCAAT 
TTGCCGAATA ACAACAATGT GGAGTCGCTT AAGAAACCGG TGGTGCAGGG AACGAGCTAC 
AATCAGACTC ATCCGCCACC GCTGATGGTT TTCAATACGG GAACAGTTGC AGTTCCCGCG 
CAGAGTCCGC AGACTGCTGC TCCACAGAAA CATTCCACCG GCAACAGCGT AGATGACAGC 
GATCTCAACG AGATACCCGT CAATGTTATC TTCAGAAAGC CGCAAGAGGC AGGCGGAGCG 
GCGAAAACAG GTGGACCGGG AGGATTAAGT GCACCTGTTT CGGGAACGCC GCAAACTCGT 
CCAGCTGAAG TGAAAATGGT GACTCCTCTC ACGCCGCCCA CTCCACCAGA GATGAGCGCA 
CCGCCCCTGC TAGCGCAAAT GCAACCCCCG CAGATACCCA CGTCTTGTGT TCCAGCTTTA 
GCTCCCAGCT TCAAAGTGTC GTCACCAGCA GTTCTCAGCC CGAAGGTGAT CTCACCAGCT 
CCTGCAAGCC CAAAGCTCTT GTGTCCGACA GCACCCGCTT CAACGAACTC ACTGCAAATT 
GCGCCAAAAG CTGTTCCAGC CACTACAACC TCATCTGCAC CAGCATTAGC CACGAAATCA 
GAACAAATGT CTTCCAAAGT GGCCAATTTA AACGCCTTCA ACCGTCAAGC ACCCATAGCT 
TCAAAGGGCG TTAGAAATGG CATCACTAAC AACAATAACA ACAGAAACAG CAATGTCGTT 
GCGAAGAAAT CGTCACCAAC TTCGATGCCG CCTCCAAAGG ATCCGATTGC ACCGATTGCA 
GCAAACGAGT TGACGGATTC CGAACATCGT CAGCGACGCC GCAAGCCCGC CTCCGCCTGC 
AGACGCAGCT TGGACACTCT GAGTGAGAAC GAGTCCTTTT CAGTGGATTC GCCCTACTGC 
CTACAACAGC ATTGGCTGCA CTCCGGCTTT AATAATAAGT CCCACGACGC GCCACTATCG 
CAAAGTAACC GGCGTGAGGA TCGAATTGCT GTTCGTAAAG GAGCCCTGAG GCGACAAGCG 
CTGCAGCTAC TGTCTACGCG CTCCCTGCAG GAGCTTCCCA TGCGAGCGGC CAAACAGCGG 
CTGCAGTGCG TCCAGAACAT GCTGATCAAG TACCAAGATC ACGCTGGCCA GCAGCAGGGA 
ATTGGAATTG GCAACCACTG TCTGGTGGCC AGCTGCAAGC AGCCCACGCT TAGTATGGCA 
GCGCACTGCG AGCGTCACAT CGTGAACAAC AGCACCCAGC AGCTTTTCCA GCCTTGCGTC 
GCCTGGCGGA TGGATGGAAC CGCTTGCCAG GCTCCTGTCT TCGATGTGCT GCACACATTG 
GCGCTCTGCA AGGTGCACAG CCACCTGCGT TCTGGTATGG ATGGAGCCCG TCCAGCATCG 
AAACAGCCGC CCGTTAGTTT ACCAGTCGCT GGAGTGGCGA CTCTTTATGT GCCTGTGAAG 
CAGCAGCGAA AGCGCAAGGC TAATACGAAT GCCGTGGCTC GTCCTCAGAA ACGTGGTAGG 
AAGCCGGCGA ATGAACCGAT TGCTAACCAG ATCAGCAGCC AGATAAACAA ACTGATACCT 
GGGGCAGGAA TGCAGCGTAA AAGCAGCACC ACATCGCTTG AGTCCATTGC CAGTAATTCG 
CAATCGTCGG CCACCTCACA CTCACAGCCG CCTTACAAGC CCGGAAATGT GTTGGCCGCT 
GTACCTGCAG CACAGAACTT GTCCCAACGG TCCATTCCTC CAGCGCTGGC TCCGCTCAGC 
AGTGATTTTC AACCCAACCA TCAGCAGCAG GAACCGCTTT TGGTTCCCAA GTTGGAGGTG 
GATTCATTGT TTAAATTTGA TGCCGATCAA CAGCAAAATC AGCAGCAGCA ATCGACTTTG 
GACCCCAGCT TACTTTCTTT GGACATTGCG AATATCAAGG CCGAGGAGAT TAGCCAAATT 
GTAGCACAAT TGGCGGCGGC CGGTGGTGAT TTACAAAATC CCAACAATAA TAATAACAAC 
AACATAGGAA TCCATAATAA CAATAGCGTG CACTTCAACA ACAACAACAA CAATAGTATG 
AACTACAGCA ACAATAACAA TAACAGTTTC CCCTCATTCA ACACGGCCTT TGGCAACGCC 
ATGGGCCAGC CAAGCAACAC AATTACGCAC AATGGCCACG CTGTTCCGGC AGTGCTGGCC 
AATACGGCCG ATCTTCTTGG CCAGGACATG TTTGGTATTT GCGAGAACAG CTCGGCATAC 
GCCAGTTCCG AGGATACCGG ATTGGGCGGT CTCAGCGAGT CGGAGCTGAT AGGCACTAAC 
GATGCTGATG ATATAGCATT GAATGGCGCC CACTTGCTGG AGGAGCACGA TCTGGTAAAT 
GTGTTCGACA CGCTGTCAGA CGATGCCTTC AACGAGCTGT TCCAATCCGT GCAACAAGCC 
GAGTGCGAGG CTATGGACCG GGCTTTGGAC CGGGCCTTAC AGCAGACAAT GGGCGGCTCG 
GCGGACAGCG CCTTTCTTAA CGATTTCCTG GACGTCGGCG ACGATCTGCT GGCCGATGCT 
GTGATGCACT CACCAAACAC GTCCGGCATC GATGCTCCTC CCCTCTTTGG GGACAGCAGC 
AGCGGCGGTG GCAATAGCAG CAGCAACGGC GCCTCCGACA TCCGGGGCTT GGTGCAGACC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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l(3)L1231 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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