put cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	put
Entrez Gene ID	41772
Full Name	punt
Synonyms	Act-r,Atr,Atr-II,Atr88CD,AtrII,CG7904,Dmel\CG7904,PUNT,Punt,Put,STK-C,TGF-B,TGF-beta,Tgf-r,atrII,dlhC,l(3)10460,l(3)j5A5,pun
General protein information	Preferred Names punt Names CG7904 gene product from transcript CG7904-RC Activin-A-receptor-88CD CG7904-PA CG7904-PB CG7904-PC DPP receptor TGF-beta-type-receptor-like activin receptor activin receptor type II dorsal holes C put-PA put-PB put-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88C9-88C9\|3-58 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275645.1	NP_001262574.1	punt, isoform C
NM_169591.3	NP_731926.1	punt, isoform A
NM_001275646.1	NP_001262575.1	punt, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACVR2A	NP_001265508.1
	ACVR2A	XP_001095960.2
Pan troglodytes (chimpanzee)	ACVR2A	XP_003949946.1
Canis lupus familiaris (dog)	ACVR2A	XP_005632022.1
Mus musculus (house mouse)	Acvr2a	NP_031422.3
Danio rerio (zebrafish)	acvr2aa	NP_001103748.1
Xenopus tropicalis (tropical clawed frog)	acvr2a	NP_001135613.1
Bos taurus (cattle)	ACVR2A	NP_776652.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000763	XP_311300.4
Rattus norvegicus (Norway rat)	Acvr2a	NP_113759.1
Gallus gallus (chicken)	ACVR2A	NP_990698.1
Drosophila melanogaster (fruit fly)	put	NP_001262574.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that members of the Activin branch of the TGFbeta signaling pathway, namely Put and Smad2, are autonomously required for cell and tissue growth in the Drosophila larval salivary gland.
Title: TGF?/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands.

requirements for TGFbeta signalling during wing disc development
Title: Activation and function of TGF? signalling during Drosophila wing development and its interactions with the BMP pathway.

In the absence of Smad2, aberrant Activin receptor signaling disrupts tissue patterning by the BMP signaling pathway.
Title: Activin receptor inhibition by Smad2 regulates Drosophila wing disc patterning through BMP-response elements.

The combined activity of the TGF-beta target genes FoxK and Dfos is critical for the direct activation of lab in the endoderm.
Title: FoxK mediates TGF-beta signalling during midgut differentiation in flies.

The following put gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the put cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04885	NM_169591.3 Latest version!	Drosophila melanogaster punt (put), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa04885	NM_001275645.1 Latest version!	Drosophila melanogaster punt (put), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07990	NM_001275646.1 Latest version!	Drosophila melanogaster punt (put), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

put ( NM_001275645.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262574.1 Drosophila melanogaster punt (put), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04885
Clone ID Related Accession (Same CDS sequence)	NM_001275645.1 , NM_169591.3
Accession Version	NM_001275645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	punt, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAAT ACGATCTGCT TTATCTAACG GCGCAGCTAA CGCTGGTCTG CTGTCTGATT 
GGAATCCATG GATCTATTTT GCCCGGAAGT CATGGGATCA TAGAATGCGA GCACTTCGAC 
GAGAAGATGT GCAACACAAC GCAGCAATGT GAAACACGGA TAGAGCACTG TAAGATGGAG 
GCGGATAAGT TTCCCAGCTG CTATGTCCTT TGGTCGGTCA ACGAGACAAC GGGCATCCTG 
CGCATCAAGA TGAAGGGCTG CTTCACGGAC ATGCACGAAT GCAATCAGAC GGAGTGCGTG 
ACCAGTGCAG AGCCACGGCA GGGAAACATT CACTTCTGCT GCTGCAAGGG ATCGCGGTGC 
AATTCCAACC AGAAATATAT TAAAAGCACC ACGGAGGCAA CCACACAAGT GCCCAAGGAG 
AAGACGCAGG ACGGCAGCAA TTTGATATAC ATCTACATTG GCACCTCCGT TTTCAGCGTG 
CTCATGGTCA TTGTTGGCAT GGGCCTTCTT CTCTACCGAC GCCGCAAGCA GGCGCACTTT 
AACGAGATAC CCACGCACGA GGCTGAGATA ACAAACTCAT CGCCATTGCT CAGCAACCGT 
CCCATTCAGC TGCTGGAACA GAAGGCCAGT GGTAGATTCG GTGATGTGTG GCAAGCCAAG 
CTCAACAATC AGGATGTGGC CGTCAAGATC TTTCGCATGC AGGAAAAAGA ATCGTGGACC 
ACGGAGCACG ATATCTACAA GCTGCCGCGC ATGCGCCATC CGAACATCCT CGAATTCCTG 
GGCGTTGAGA AGCACATGGA CAAGCCGGAA TATTGGCTGA TATCCACCTA CCAGCATAAC 
GGATCACTAT GCGACTACCT CAAATCGCAC ACGATCTCAT GGCCAGAGTT GTGCCGCATC 
GCTGAGTCCA TGGCCAATGG ACTGGCACAT CTGCACGAGG AGATCCCGGC ATCAAAGACC 
GATGGGCTAA AACCATCGAT AGCTCACCGA GACTTCAAGT CTAAGAACGT ACTGCTTAAG 
AGCGATCTGA CGGCCTGTAT AGCTGACTTT GGTTTGGCCA TGATATTCCA GCCAGGCAAG 
CCCTGCGGCG ATACACACGG TCAAGTAGGC ACTCGACGTT ACATGGCCCC AGAGGTGCTT 
GAGGGTGCCA TCAATTTCAA TAGAGACGCT TTCTTACGCA TAGACGTCTA CGCATGCGGC 
CTAGTCCTCT GGGAAATGGT GTCACGGTGT GACTTTGCCG GACCCGTCGG TGAGTTCCAG 
CTGCCTTTTG AGGCCGAGCT GGGCCTGAGG CCGTCGCTGG ACGAAGTTCA GGAGAGTGTG 
GTAATGAAGA AGCTGCGCCC TCGTTTGCTC AACTCCTGGC GCGCCCATCC GGGACTTAAT 
GTATTCTGCG ACACAATGGA GGAGTGCTGG GATCACGACG CTGAGGCTCG TCTTAGCTCT 
TCGTGTGTGA TGGAACGCTT TGCGCAGCTA AACAAGTATC CCTCAACCCA GTTGCTAATC 
AAGAACCACA CCAACATTGA CGACGCCAAG GAATCTACGA ATTGCTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262574.1
CDS	459..2009
Translation	MSKYDLLYLTAQLTLVCCLIGIHGSILPGSHGIIECEHFDEKMCNTTQQCETRIEHCKMEADKFPSCYVLWSVNETTGILRIKMKGCFTDMHECNQTECVTSAEPRQGNIHFCCCKGSRCNSNQKYIKSTTEATTQVPKEKTQDGSNLIYIYIGTSVFSVLMVIVGMGLLLYRRRKQAHFNEIPTHEAEITNSSPLLSNRPIQLLEQKASGRFGDVWQAKLNNQDVAVKIFRMQEKESWTTEHDIYKLPRMRHPNILEFLGVEKHMDKPEYWLISTYQHNGSLCDYLKSHTISWPELCRIAESMANGLAHLHEEIPASKTDGLKPSIAHRDFKSKNVLLKSDLTACIADFGLAMIFQPGKPCGDTHGQVGTRRYMAPEVLEGAINFNRDAFLRIDVYACGLVLWEMVSRCDFAGPVGEFQLPFEAELGLRPSLDEVQESVVMKKLRPRLLNSWRAHPGLNVFCDTMEECWDHDAEARLSSSCVMERFAQLNKYPSTQLLIKNHTNIDDAKESTNCL

Target ORF information:

RefSeq Version	NM_001275645.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster punt (put), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275645.1

ORF Insert Sequence:

ATGTCCAAAT ACGATCTGCT TTATCTAACG GCGCAGCTAA CGCTGGTCTG CTGTCTGATT 
GGAATCCATG GATCTATTTT GCCCGGAAGT CATGGGATCA TAGAATGCGA GCACTTCGAC 
GAGAAGATGT GCAACACAAC GCAGCAATGT GAAACACGGA TAGAGCACTG TAAGATGGAG 
GCGGATAAGT TTCCCAGCTG CTATGTCCTT TGGTCGGTCA ACGAGACAAC GGGCATCCTG 
CGCATCAAGA TGAAGGGCTG CTTCACGGAC ATGCACGAAT GCAATCAGAC GGAGTGCGTG 
ACCAGTGCAG AGCCACGGCA GGGAAACATT CACTTCTGCT GCTGCAAGGG ATCGCGGTGC 
AATTCCAACC AGAAATATAT TAAAAGCACC ACGGAGGCAA CCACACAAGT GCCCAAGGAG 
AAGACGCAGG ACGGCAGCAA TTTGATATAC ATCTACATTG GCACCTCCGT TTTCAGCGTG 
CTCATGGTCA TTGTTGGCAT GGGCCTTCTT CTCTACCGAC GCCGCAAGCA GGCGCACTTT 
AACGAGATAC CCACGCACGA GGCTGAGATA ACAAACTCAT CGCCATTGCT CAGCAACCGT 
CCCATTCAGC TGCTGGAACA GAAGGCCAGT GGTAGATTCG GTGATGTGTG GCAAGCCAAG 
CTCAACAATC AGGATGTGGC CGTCAAGATC TTTCGCATGC AGGAAAAAGA ATCGTGGACC 
ACGGAGCACG ATATCTACAA GCTGCCGCGC ATGCGCCATC CGAACATCCT CGAATTCCTG 
GGCGTTGAGA AGCACATGGA CAAGCCGGAA TATTGGCTGA TATCCACCTA CCAGCATAAC 
GGATCACTAT GCGACTACCT CAAATCGCAC ACGATCTCAT GGCCAGAGTT GTGCCGCATC 
GCTGAGTCCA TGGCCAATGG ACTGGCACAT CTGCACGAGG AGATCCCGGC ATCAAAGACC 
GATGGGCTAA AACCATCGAT AGCTCACCGA GACTTCAAGT CTAAGAACGT ACTGCTTAAG 
AGCGATCTGA CGGCCTGTAT AGCTGACTTT GGTTTGGCCA TGATATTCCA GCCAGGCAAG 
CCCTGCGGCG ATACACACGG TCAAGTAGGC ACTCGACGTT ACATGGCCCC AGAGGTGCTT 
GAGGGTGCCA TCAATTTCAA TAGAGACGCT TTCTTACGCA TAGACGTCTA CGCATGCGGC 
CTAGTCCTCT GGGAAATGGT GTCACGGTGT GACTTTGCCG GACCCGTCGG TGAGTTCCAG 
CTGCCTTTTG AGGCCGAGCT GGGCCTGAGG CCGTCGCTGG ACGAAGTTCA GGAGAGTGTG 
GTAATGAAGA AGCTGCGCCC TCGTTTGCTC AACTCCTGGC GCGCCCATCC GGGACTTAAT 
GTATTCTGCG ACACAATGGA GGAGTGCTGG GATCACGACG CTGAGGCTCG TCTTAGCTCT 
TCGTGTGTGA TGGAACGCTT TGCGCAGCTA AACAAGTATC CCTCAACCCA GTTGCTAATC 
AAGAACCACA CCAACATTGA CGACGCCAAG GAATCTACGA ATTGCTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

put ( NM_169591.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731926.1 Drosophila melanogaster punt (put), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04885
Clone ID Related Accession (Same CDS sequence)	NM_001275645.1 , NM_169591.3
Accession Version	NM_169591.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	punt, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169591.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAAT ACGATCTGCT TTATCTAACG GCGCAGCTAA CGCTGGTCTG CTGTCTGATT 
GGAATCCATG GATCTATTTT GCCCGGAAGT CATGGGATCA TAGAATGCGA GCACTTCGAC 
GAGAAGATGT GCAACACAAC GCAGCAATGT GAAACACGGA TAGAGCACTG TAAGATGGAG 
GCGGATAAGT TTCCCAGCTG CTATGTCCTT TGGTCGGTCA ACGAGACAAC GGGCATCCTG 
CGCATCAAGA TGAAGGGCTG CTTCACGGAC ATGCACGAAT GCAATCAGAC GGAGTGCGTG 
ACCAGTGCAG AGCCACGGCA GGGAAACATT CACTTCTGCT GCTGCAAGGG ATCGCGGTGC 
AATTCCAACC AGAAATATAT TAAAAGCACC ACGGAGGCAA CCACACAAGT GCCCAAGGAG 
AAGACGCAGG ACGGCAGCAA TTTGATATAC ATCTACATTG GCACCTCCGT TTTCAGCGTG 
CTCATGGTCA TTGTTGGCAT GGGCCTTCTT CTCTACCGAC GCCGCAAGCA GGCGCACTTT 
AACGAGATAC CCACGCACGA GGCTGAGATA ACAAACTCAT CGCCATTGCT CAGCAACCGT 
CCCATTCAGC TGCTGGAACA GAAGGCCAGT GGTAGATTCG GTGATGTGTG GCAAGCCAAG 
CTCAACAATC AGGATGTGGC CGTCAAGATC TTTCGCATGC AGGAAAAAGA ATCGTGGACC 
ACGGAGCACG ATATCTACAA GCTGCCGCGC ATGCGCCATC CGAACATCCT CGAATTCCTG 
GGCGTTGAGA AGCACATGGA CAAGCCGGAA TATTGGCTGA TATCCACCTA CCAGCATAAC 
GGATCACTAT GCGACTACCT CAAATCGCAC ACGATCTCAT GGCCAGAGTT GTGCCGCATC 
GCTGAGTCCA TGGCCAATGG ACTGGCACAT CTGCACGAGG AGATCCCGGC ATCAAAGACC 
GATGGGCTAA AACCATCGAT AGCTCACCGA GACTTCAAGT CTAAGAACGT ACTGCTTAAG 
AGCGATCTGA CGGCCTGTAT AGCTGACTTT GGTTTGGCCA TGATATTCCA GCCAGGCAAG 
CCCTGCGGCG ATACACACGG TCAAGTAGGC ACTCGACGTT ACATGGCCCC AGAGGTGCTT 
GAGGGTGCCA TCAATTTCAA TAGAGACGCT TTCTTACGCA TAGACGTCTA CGCATGCGGC 
CTAGTCCTCT GGGAAATGGT GTCACGGTGT GACTTTGCCG GACCCGTCGG TGAGTTCCAG 
CTGCCTTTTG AGGCCGAGCT GGGCCTGAGG CCGTCGCTGG ACGAAGTTCA GGAGAGTGTG 
GTAATGAAGA AGCTGCGCCC TCGTTTGCTC AACTCCTGGC GCGCCCATCC GGGACTTAAT 
GTATTCTGCG ACACAATGGA GGAGTGCTGG GATCACGACG CTGAGGCTCG TCTTAGCTCT 
TCGTGTGTGA TGGAACGCTT TGCGCAGCTA AACAAGTATC CCTCAACCCA GTTGCTAATC 
AAGAACCACA CCAACATTGA CGACGCCAAG GAATCTACGA ATTGCTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731926.1
CDS	459..2009
Translation	MSKYDLLYLTAQLTLVCCLIGIHGSILPGSHGIIECEHFDEKMCNTTQQCETRIEHCKMEADKFPSCYVLWSVNETTGILRIKMKGCFTDMHECNQTECVTSAEPRQGNIHFCCCKGSRCNSNQKYIKSTTEATTQVPKEKTQDGSNLIYIYIGTSVFSVLMVIVGMGLLLYRRRKQAHFNEIPTHEAEITNSSPLLSNRPIQLLEQKASGRFGDVWQAKLNNQDVAVKIFRMQEKESWTTEHDIYKLPRMRHPNILEFLGVEKHMDKPEYWLISTYQHNGSLCDYLKSHTISWPELCRIAESMANGLAHLHEEIPASKTDGLKPSIAHRDFKSKNVLLKSDLTACIADFGLAMIFQPGKPCGDTHGQVGTRRYMAPEVLEGAINFNRDAFLRIDVYACGLVLWEMVSRCDFAGPVGEFQLPFEAELGLRPSLDEVQESVVMKKLRPRLLNSWRAHPGLNVFCDTMEECWDHDAEARLSSSCVMERFAQLNKYPSTQLLIKNHTNIDDAKESTNCL

Target ORF information:

RefSeq Version	NM_169591.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster punt (put), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169591.3

ORF Insert Sequence:

ATGTCCAAAT ACGATCTGCT TTATCTAACG GCGCAGCTAA CGCTGGTCTG CTGTCTGATT 
GGAATCCATG GATCTATTTT GCCCGGAAGT CATGGGATCA TAGAATGCGA GCACTTCGAC 
GAGAAGATGT GCAACACAAC GCAGCAATGT GAAACACGGA TAGAGCACTG TAAGATGGAG 
GCGGATAAGT TTCCCAGCTG CTATGTCCTT TGGTCGGTCA ACGAGACAAC GGGCATCCTG 
CGCATCAAGA TGAAGGGCTG CTTCACGGAC ATGCACGAAT GCAATCAGAC GGAGTGCGTG 
ACCAGTGCAG AGCCACGGCA GGGAAACATT CACTTCTGCT GCTGCAAGGG ATCGCGGTGC 
AATTCCAACC AGAAATATAT TAAAAGCACC ACGGAGGCAA CCACACAAGT GCCCAAGGAG 
AAGACGCAGG ACGGCAGCAA TTTGATATAC ATCTACATTG GCACCTCCGT TTTCAGCGTG 
CTCATGGTCA TTGTTGGCAT GGGCCTTCTT CTCTACCGAC GCCGCAAGCA GGCGCACTTT 
AACGAGATAC CCACGCACGA GGCTGAGATA ACAAACTCAT CGCCATTGCT CAGCAACCGT 
CCCATTCAGC TGCTGGAACA GAAGGCCAGT GGTAGATTCG GTGATGTGTG GCAAGCCAAG 
CTCAACAATC AGGATGTGGC CGTCAAGATC TTTCGCATGC AGGAAAAAGA ATCGTGGACC 
ACGGAGCACG ATATCTACAA GCTGCCGCGC ATGCGCCATC CGAACATCCT CGAATTCCTG 
GGCGTTGAGA AGCACATGGA CAAGCCGGAA TATTGGCTGA TATCCACCTA CCAGCATAAC 
GGATCACTAT GCGACTACCT CAAATCGCAC ACGATCTCAT GGCCAGAGTT GTGCCGCATC 
GCTGAGTCCA TGGCCAATGG ACTGGCACAT CTGCACGAGG AGATCCCGGC ATCAAAGACC 
GATGGGCTAA AACCATCGAT AGCTCACCGA GACTTCAAGT CTAAGAACGT ACTGCTTAAG 
AGCGATCTGA CGGCCTGTAT AGCTGACTTT GGTTTGGCCA TGATATTCCA GCCAGGCAAG 
CCCTGCGGCG ATACACACGG TCAAGTAGGC ACTCGACGTT ACATGGCCCC AGAGGTGCTT 
GAGGGTGCCA TCAATTTCAA TAGAGACGCT TTCTTACGCA TAGACGTCTA CGCATGCGGC 
CTAGTCCTCT GGGAAATGGT GTCACGGTGT GACTTTGCCG GACCCGTCGG TGAGTTCCAG 
CTGCCTTTTG AGGCCGAGCT GGGCCTGAGG CCGTCGCTGG ACGAAGTTCA GGAGAGTGTG 
GTAATGAAGA AGCTGCGCCC TCGTTTGCTC AACTCCTGGC GCGCCCATCC GGGACTTAAT 
GTATTCTGCG ACACAATGGA GGAGTGCTGG GATCACGACG CTGAGGCTCG TCTTAGCTCT 
TCGTGTGTGA TGGAACGCTT TGCGCAGCTA AACAAGTATC CCTCAACCCA GTTGCTAATC 
AAGAACCACA CCAACATTGA CGACGCCAAG GAATCTACGA ATTGCTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

put ( NM_001275646.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262575.1 Drosophila melanogaster punt (put), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07990
Clone ID Related Accession (Same CDS sequence)	NM_001275646.1
Accession Version	NM_001275646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	punt, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAAT ACGATCTGCT TTATCTAACG GCGCAGCTAA CGCTGGTCTG CTGTCTGATT 
GGAATCCATG GATCTATTTT GCCCGGAAGT CATGGGATCA TAGAATGCGA GCACTTCGAC 
GAGAAGATGT GCAACACAAC GCAGCAATGT GAAACACGGA TAGAGCACTG TAAGATGGAG 
GCGGATAAGT TTCCCAGCTG CTATGTCCTT TGGTCGGTCA ACGAGACAAC GGGCATCCTG 
CGCATCAAGA TGAAGGGCTG CTTCACGGAC ATGCACGAAT GCAATCAGAC GGAGTGCGTG 
ACCAGTGCAG AGCCACGGCA GGGAAACATT CACTTCTGCT GCTGCAAGGG ATCGCGGTGC 
AATTCCAACC AGAAATATAT TAAAAGCACC ACGGAGGCAA CCACACAAGT GCCCAAGGAG 
AAGACGCAGG ACGGCAGCAA TTTGATATAC ATCTACATTG GCACCTCCGT TTTCAGCGTG 
CTCATGGTCA TTGTTGGCAT GGGCCTTCTT CTCTACCGAC GCCGCAAGCA GGCGCACTTT 
AACGAGATAC CCACGCACGA GGCTGAGATA ACAAACTCAT CGCCATTGCT CAGCAACCGT 
CCCATTCAGC TGCTGGAACA GAAGGCCAGT GGTAGATTCG GTGATGTGTG GCAAGCCAAG 
CTCAACAATC AGGATGTGGC CGTCAAGATC TTTCGCATGC AGGAAAAAGA ATCGTGGACC 
ACGGAGCACG ATATCTACAA GCTGCCGCGC ATGCGCCATC CGAACATCCT CGAATTCCTG 
GGCGTTGAGA AGCACATGGA CAAGCCGGAA TATTGGCTGA TATCCACCTA CCAGCATAAC 
GGATCACTAT GCGACTACCT CAAATCGCAC ACGATCTCAT GGCCAGAGTT GTGCCGCATC 
GCTGAGTCCA TGGCCAATGG ACTGGCACAT CTGCACGAGG AGATCCCGGC ATCAAAGACC 
GATGGGCTAA AACCATCGAT AGCTCACCGA GACTTCAAGT CTAAGAACGT ACTGCTTAAG 
AGCGATCTGA CGGCCTGTAT AGCTGACTTT GGTTTGGCCA TGATATTCCA GCCAGGCAAG 
CCCTGCGGCG ATACACACGG TCAAGTAGGC ACTCGACGTT ACATGGCCCC AGAGGTGCTT 
GAGGGTGCCA TCAATTTCAA TAGAGACGCT TTCTTACGCA TAGACGTCTA CGCATGCGGC 
CTAGTCCTCT GGGAAATGGT GTCACGGTGT GACTTTGCCG GACCCGTCGG TGAGTTCCAG 
CTGCCTTTTG AGGCCGAGCT GGGCCTGAGG CCGTCGCTGG ACGAAGTTCA GGAGAGTGTG 
GTAATGAAGA AGCTGCGCCC TCGTTTGCTC AACTCCTGGC GCGCCCATCC GGGACTTAAT 
GTATTCTGCG ACACAATGGA GGAGTGCTGG GATCACGACG CTGAGGCTCG TCTTAGCTCT 
TCGTGTGTGA TGGAACGCTT TGCGCAGCTA AACAAGTATC CCTCAACCCA GTTGCTAATC 
AAGAACCACA CCAACATTGA CGACGCCAAG GAATCTACGA ATTGCTTATA GAAGCGGTAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262575.1
CDS	459..2021
Translation	MSKYDLLYLTAQLTLVCCLIGIHGSILPGSHGIIECEHFDEKMCNTTQQCETRIEHCKMEADKFPSCYVLWSVNETTGILRIKMKGCFTDMHECNQTECVTSAEPRQGNIHFCCCKGSRCNSNQKYIKSTTEATTQVPKEKTQDGSNLIYIYIGTSVFSVLMVIVGMGLLLYRRRKQAHFNEIPTHEAEITNSSPLLSNRPIQLLEQKASGRFGDVWQAKLNNQDVAVKIFRMQEKESWTTEHDIYKLPRMRHPNILEFLGVEKHMDKPEYWLISTYQHNGSLCDYLKSHTISWPELCRIAESMANGLAHLHEEIPASKTDGLKPSIAHRDFKSKNVLLKSDLTACIADFGLAMIFQPGKPCGDTHGQVGTRRYMAPEVLEGAINFNRDAFLRIDVYACGLVLWEMVSRCDFAGPVGEFQLPFEAELGLRPSLDEVQESVVMKKLRPRLLNSWRAHPGLNVFCDTMEECWDHDAEARLSSSCVMERFAQLNKYPSTQLLIKNHTNIDDAKESTNCL*KRY

Target ORF information:

RefSeq Version	NM_001275646.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster punt (put), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275646.1

ORF Insert Sequence:

ATGTCCAAAT ACGATCTGCT TTATCTAACG GCGCAGCTAA CGCTGGTCTG CTGTCTGATT 
GGAATCCATG GATCTATTTT GCCCGGAAGT CATGGGATCA TAGAATGCGA GCACTTCGAC 
GAGAAGATGT GCAACACAAC GCAGCAATGT GAAACACGGA TAGAGCACTG TAAGATGGAG 
GCGGATAAGT TTCCCAGCTG CTATGTCCTT TGGTCGGTCA ACGAGACAAC GGGCATCCTG 
CGCATCAAGA TGAAGGGCTG CTTCACGGAC ATGCACGAAT GCAATCAGAC GGAGTGCGTG 
ACCAGTGCAG AGCCACGGCA GGGAAACATT CACTTCTGCT GCTGCAAGGG ATCGCGGTGC 
AATTCCAACC AGAAATATAT TAAAAGCACC ACGGAGGCAA CCACACAAGT GCCCAAGGAG 
AAGACGCAGG ACGGCAGCAA TTTGATATAC ATCTACATTG GCACCTCCGT TTTCAGCGTG 
CTCATGGTCA TTGTTGGCAT GGGCCTTCTT CTCTACCGAC GCCGCAAGCA GGCGCACTTT 
AACGAGATAC CCACGCACGA GGCTGAGATA ACAAACTCAT CGCCATTGCT CAGCAACCGT 
CCCATTCAGC TGCTGGAACA GAAGGCCAGT GGTAGATTCG GTGATGTGTG GCAAGCCAAG 
CTCAACAATC AGGATGTGGC CGTCAAGATC TTTCGCATGC AGGAAAAAGA ATCGTGGACC 
ACGGAGCACG ATATCTACAA GCTGCCGCGC ATGCGCCATC CGAACATCCT CGAATTCCTG 
GGCGTTGAGA AGCACATGGA CAAGCCGGAA TATTGGCTGA TATCCACCTA CCAGCATAAC 
GGATCACTAT GCGACTACCT CAAATCGCAC ACGATCTCAT GGCCAGAGTT GTGCCGCATC 
GCTGAGTCCA TGGCCAATGG ACTGGCACAT CTGCACGAGG AGATCCCGGC ATCAAAGACC 
GATGGGCTAA AACCATCGAT AGCTCACCGA GACTTCAAGT CTAAGAACGT ACTGCTTAAG 
AGCGATCTGA CGGCCTGTAT AGCTGACTTT GGTTTGGCCA TGATATTCCA GCCAGGCAAG 
CCCTGCGGCG ATACACACGG TCAAGTAGGC ACTCGACGTT ACATGGCCCC AGAGGTGCTT 
GAGGGTGCCA TCAATTTCAA TAGAGACGCT TTCTTACGCA TAGACGTCTA CGCATGCGGC 
CTAGTCCTCT GGGAAATGGT GTCACGGTGT GACTTTGCCG GACCCGTCGG TGAGTTCCAG 
CTGCCTTTTG AGGCCGAGCT GGGCCTGAGG CCGTCGCTGG ACGAAGTTCA GGAGAGTGTG 
GTAATGAAGA AGCTGCGCCC TCGTTTGCTC AACTCCTGGC GCGCCCATCC GGGACTTAAT 
GTATTCTGCG ACACAATGGA GGAGTGCTGG GATCACGACG CTGAGGCTCG TCTTAGCTCT 
TCGTGTGTGA TGGAACGCTT TGCGCAGCTA AACAAGTATC CCTCAACCCA GTTGCTAATC 
AAGAACCACA CCAACATTGA CGACGCCAAG GAATCTACGA ATTGCTTATA GAAGCGGTAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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put cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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